Midwestern Streams Research Articles

Shifting climate conditions affect recruitment in Midwestern stream trout, but depend on seasonal and spatial context

AbstractClimate change is a complex threat to freshwater ecosystems. Effects on aquatic species will likely differ among populations depending on seasonal and spatial context, which makes a detailed understanding of population responses to shifting climate conditions key to guiding strategic decision‐making. However, few empirical studies have tested for such context dependency on distinct populations across seasons or at large spatial scales. We used 26 years of standardized survey data on recreationally and economically important brook trout (Salvelinus fontinalis) and brown trout (Salmo trutta) in Wisconsin, USA to assess short‐ and long‐term variability in population trends, and quantify the influence of seasonal and spatial climate variability (air temperatures and precipitation) on annual recruitment strength (as indexed by young‐of‐year [YOY] relative abundance in summer). Some short‐term population fluctuations were spatially consistent across the state, indicative of broadscale environmental forcing. Over the longer term, average YOY and age 1 and older brook trout relative abundance has declined since 2006, especially for YOY, while brown trout abundance has substantially increased. The effects of climate conditions on recruitment varied by species, season, and latitude. Increasing maximum summer temperatures were associated with lower brook trout recruitment, but higher brown trout recruitment. The effect for both species was stronger at lower latitudes. Spring temperatures were positively related to brown trout recruitment at lower latitudes; in mid‐latitude and northern streams, they were related to increasing recruitment up to about 1 standard deviation, above which recruitment declined. High and low winter and spring precipitation were associated with precipitous declines in recruitment for both species. By contrast, summer precipitation was positively related to recruitment in northern streams for brook trout and southern streams for brown trout. Our results demonstrate that shifts in climate can affect recruitment in similar species differently depending on temporal (seasonal) and spatial (warm, southern regions compared with cool, northern regions) context. Given trout population trends in Wisconsin and climate projections for the Midwestern United States, location‐ and species‐specific management actions are needed that account for this context dependency. Management should aim to maximize the resiliency of populations to extreme climate conditions by buffering negative influences on recruitment.

Variable Background Flow on Aquatic Toxicant Exposure Alters Foraging Patterns on Crayfish.

Climate change is expected to alter hydrological cycles on global and regional scales, impacting groundwater and surface water inputs to stream habitats. In the midwestern United States, the volume and frequency of inputs are expected to become increasingly variable. This region has a high incidence of agriculture, creating enormous potential for transport of pesticides and herbicides into aquatic ecosystems. Metolachlor, an herbicide for corn and soybean crops, has been demonstrated to contaminate surface water and groundwater in the region. This study examines the impact of variable flow conditions on the toxicity of environmentally relevant concentrations of metolachlor in a macroinvertebrate found in midwestern streams, the rusty crayfish (Faxonius rusticus). Changes in crayfish foraging behavior were analyzed using a Mixed Model ANCOVA. Under toxicant exposure, crayfish significantly increased their consumption of macrophytes, but only under the variable flow regime. Thus, the increased variability in toxicant exposure impacted crayfish foraging behavior more than other flow regimes. This significant interaction between flow regime and metolachlor exposure suggests that the greater variability in toxicant inputs to streams may lead to more severe changes in behavior for exposed organisms.

Seasonal differences in diversity of macroinvertebrate and fish taxa in a Midwestern stream

AbstractMost biomonitoring projects focus on single species groups at time scales either too short or too coarse to detect intra‐annual oscillations in biodiversity. Using a multifaceted approach, we compared diversity indices of larval macroinvertebrate families and fish species in a Midwestern stream during spring and fall of 2009–2013 and discovered contrasting patterns in α and β diversities between the seasons for the two taxa groups. Compared with spring, both α and β diversities were significantly higher during fall for macroinvertebrates; on the contrary, only α diversity differed between the seasons for fishes. For both taxa, we partitioned the overall β diversity to identify contributions of temporal and spatial β diversities on the observed differences. The observed patterns for macroinvertebrates were likely the result of season acting as environmental filter, but stochastic dispersal and recruitment processes were likely more important in driving fish diversity patterns. In light of widespread conservation and restoration efforts in the Midwestern streams, it seems prudent to study community composition frequently so that baseline α and β diversities can be obtained for organisms at different taxonomic levels and during different seasons.

Statistical assessment of nonpoint source pollution in agricultural watersheds in the Lower Grand River watershed, MO, USA

The water quality in many Midwestern streams and lakes is negatively impacted by agricultural activities. Although the agricultural inputs that degrade water quality are well known, the impact of these inputs varies as a function of geologic and topographic parameters. To better understand how a range of land use, geologic, and topographic factors affect water quality in Midwestern watersheds, we sampled surface water quality parameters, including nitrate, phosphate, dissolved oxygen, turbidity, bacteria, pH, specific conductance, temperature, and biotic index (BI) in 35 independent sub-watersheds within the Lower Grand River Watershed in northern Missouri. For each sub-watershed, the land use/land cover, soil texture, depth to bedrock, depth to the water table, recent precipitation area, total stream length, watershed shape/relief ratio, topographic complexity, mean elevation, and slope were determined. Water quality sampling was conducted twice: in the spring and in the late summer/early fall. A pairwise comparison of water quality parameters acquired in the fall and spring showed that each of these factors varies considerably with season, suggesting that the timing is critical when comparing water quality indicators. Correlation analysis between water quality indicators and watershed characteristics revealed that both geologic and land use characteristics correlated significantly with water quality parameters. The water quality index had the highest correlation with the biotic index during the spring, implying that the lower water quality conditions observed in the spring might be more representative of the longer-term water quality conditions in these watersheds than the higher quality conditions observed in the fall. An assessment of macroinvertebrates indicated that the biotic index was primarily influenced by nutrient loading due to excessive amounts of phosphorus (P) and nitrogen (N) discharge from agricultural land uses. The PCA analysis found a correlation between turbidity, E. coli, and BI, suggesting that livestock grazing may adversely affect the water quality in this watershed. Moreover, this analysis found that N, P, and SC contribute greatly to the observed water quality variability. The results of this study can be used to improve decision-making strategies to improve water quality for the entire river basin.

Factors Influencing Fine Sediment on Stream Beds in the Midwestern United States.

Fine sediment (particles <2 mm in diameter) in stream beds has wide-ranging effects on hydraulics, geomorphology, and ecology and is a primary focus for stream quality management in many regions. We identify reach- and basin-scale factors associated with fine sediment in the beds of 83 stream reaches in the Midwestern United States using recursive partitioning of sand-bed and gravel-bed streams and a generalized linear model for the fraction of a stream bed covered by fine sediment. A water-surface gradient of 0.00075 is the best single determinant (80% correct classification) distinguishing sand-bed streams (lower gradient) from gravel-bed streams (higher gradient). In the higher gradient category, sand-bed streams generally had more variable monthly precipitation than gravel-bed streams. The fractional response model indicated that the proportion of a stream bed composed of fine sediment is related to high sediment supply and low transport capacity but also high gravel transport capacity. This result is consistent with both theory and observations that bed material can be transported indiscriminately with respect to particle size under high shear stress, which will drive the particle size distribution of bed material toward the distribution of supply. Management of fine sediment in Midwestern streams has been approached largely by focusing on sediment supply, which may be immutable in some places due to the landscape position or glacial history. Retention of coarse sediment is an alternative management approach to reduce the fraction of fine sediment in the beds of some Midwestern streams.

Relative Importance of Water‐Quality Stressors in Predicting Fish Community Responses in Midwestern Streams

AbstractFish, habitat, and water chemistry data were collected from 98 streams in the midwestern United States, an area dominated by intense cultivation of row crops, in order to identify important water‐quality stressors to fish communities. We focused on 10 stressors including riparian disturbance, riparian vegetative cover, instream fish cover, streambed sedimentation, streamflow variability, total nitrogen, total phosphorus, minimum dissolved oxygen, pesticides, and bed sediment contaminants. Fish community response variables included a measure of observed/expected taxonomic completeness; species‐specific tolerances to nitrogen, phosphorus, dissolved oxygen, and water temperature; the percent of species classified as macrohabitat generalists; and an index of pesticide toxicity to fish. Multivariate analysis indicated that total nitrogen was the most important stressor, signifying that fish communities were responding to total nitrogen despite relatively high levels common to an agricultural setting. Individually, fish taxonomic completeness decreased with increasing streambed sedimentation, whereas fish community tolerance to total phosphorus increased with increasing streambed sedimentation, riparian disturbance, and total nitrogen. These findings underscore the importance of multiple biological response metrics to better understand the effects of water‐quality stressors on fish communities and highlight the complex relations between total phosphorus and fish communities.

Winter Habitat Selection by Large Brown Trout in Streams with and without Habitat Rehabilitation

AbstractTo determine whether habitat rehabilitation provides functionally similar winter habitat to streams with natural features, we radio‐tagged and tracked 83 large, stream‐resident, Brown Trout Salmo trutta (&gt;330 mm TL) and compared habitat use at pool and microhabitat scales. Brown Trout used pools similarly in streams with and without habitat rehabilitation. In all streams, trout avoided pools lacking depths &gt;60 cm and with &lt;10 m2 of cover. Streams with habitat rehabilitation provided similar microhabitat features to natural streams; trout selected sites with depths from 60 to 119 cm near woody debris and with water column velocity &lt;10 cm/s in both. Trout avoided microhabitats with depths &lt;60 cm when the habitats either lacked cover or were under ice shelves or had a water column velocity &gt; 20 cm/s. Brown Trout selected artificially placed overhead bank structures and instream rocks in rehabilitated streams but used these natural features in proportion to their availability in streams without rehabilitation. We conclude that habitat rehabilitation created suitable winter habitat for Brown Trout in upper U.S. Midwestern streams.

Complex mixtures of dissolved pesticides show potential aquatic toxicity in a synoptic study of Midwestern U.S. streams

Aquatic organisms in streams are exposed to pesticide mixtures that vary in composition over time in response to changes in flow conditions, pesticide inputs to the stream, and pesticide fate and degradation within the stream. To characterize mixtures of dissolved-phase pesticides and degradates in Midwestern streams, a synoptic study was conducted at 100 streams during May–August 2013. In weekly water samples, 94 pesticides and 89 degradates were detected, with a median of 25 compounds detected per sample and 54 detected per site. In a screening-level assessment using aquatic-life benchmarks and the Pesticide Toxicity Index (PTI), potential effects on fish were unlikely in most streams. For invertebrates, potential chronic toxicity was predicted in 53% of streams, punctuated in 12% of streams by acutely toxic exposures. For aquatic plants, acute but likely reversible effects on biomass were predicted in 75% of streams, with potential longer-term effects on plant communities in 9% of streams. Relatively few pesticides in water—atrazine, acetochlor, metolachlor, imidacloprid, fipronil, organophosphate insecticides, and carbendazim—were predicted to be major contributors to potential toxicity. Agricultural streams had the highest potential for effects on plants, especially in May–June, corresponding to high spring-flush herbicide concentrations. Urban streams had higher detection frequencies and concentrations of insecticides and most fungicides than in agricultural streams, and higher potential for invertebrate toxicity, which peaked during July–August. Toxicity-screening predictions for invertebrates were supported by quantile regressions showing significant associations for the Benthic Invertebrate-PTI and imidacloprid concentrations with invertebrate community metrics for MSQA streams, and by mesocosm toxicity testing with imidacloprid showing effects on invertebrate communities at environmentally relevant concentrations. This study documents the most complex pesticide mixtures yet reported in discrete water samples in the U.S. and, using multiple lines of evidence, predicts that pesticides were potentially toxic to nontarget aquatic life in about half of the sampled streams.

Understanding mass fluvial erosion along a bank profile: using PEEP technology for quantifying retreat lengths and identifying event timing

AbstractThis study provides fundamental examination of mass fluvial erosion along a stream bank by identifying event timing, quantifying retreat lengths, and providing ranges of incipient shear stress for hydraulically driven erosion. Mass fluvial erosion is defined here as the detachment of thin soil layers or conglomerates from the bank face under higher hydraulic shear stresses relative to surface fluvial erosion, or the entrainment of individual grains or aggregates under lower hydraulic shear stresses.We explore the relationship between the two regimes in a representative, US Midwestern stream with semi‐cohesive bank soils, namely Clear Creek, IA. Photo‐Electronic Erosion Pins (PEEPs) provide, for the first time, in situ measurements of mass fluvial erosion retreat lengths during a season. The PEEPs were installed at identical locations where surface fluvial erosion measurements exist for identifying the transition point between the two regimes. This transition is postulated to occur when the applied shear stress surpasses a second threshold, namely the critical shear stress for mass fluvial erosion.We hypothesize that the regimes are intricately related and surface fluvial erosion can facilitate mass fluvial erosion. Selective entrainment of unbound/exposed, mostly silt‐sized particles at low shear stresses over sand‐sized sediment can armor the bank surface, limiting the removal of the underlying soil. The armoring here is enhanced by cementation from the presence of optimal levels of sand and clay. Select studies show that fluvial erosion strength can increase several‐fold when appropriate amounts of sand and clay are mixed and cement together. Hence, soil layers or conglomerates are entrained with higher flows.The critical shear stress for mass fluvial erosion was found to be an order of magnitude higher than that of surface fluvial erosion, and proceeded with higher (approximately 2–4 times) erodibility. The results were well represented by a mechanistic detachment model that captures the two regimes. Copyright © 2017 John Wiley &amp; Sons, Ltd.

Similarities and differences in occurrence and temporal fluctuations in glyphosate and atrazine in small Midwestern streams (USA) during the 2013 growing season

Glyphosate and atrazine are the most intensively used herbicides in the United States. Although there is abundant spatial and temporal information on atrazine occurrence at regional scales, there are far fewer data for glyphosate, and studies that compare the two herbicides are rare. We investigated temporal patterns in glyphosate and atrazine concentrations measured weekly during the 2013 growing season in 100 small streams in the Midwestern United States. Glyphosate was detected in 44% of samples (method reporting level 0.2μg/L); atrazine was detected above a threshold of 0.2μg/L in 54% of samples. Glyphosate was detected more frequently in 12 urban streams than in 88 agricultural streams, and at concentrations similar to those in streams with high agricultural land use (>40% row crop) in the watershed. In contrast, atrazine was detected more frequently and at higher concentrations in agricultural streams than in urban streams. The maximum concentration of glyphosate measured at most urban sites exceeded the maximum atrazine concentration, whereas at agricultural sites the reverse was true. Measurement at a 2-day interval at 8 sites in northern Missouri revealed that transport of both herbicide compounds appeared to be controlled by spring flush, that peak concentration duration was brief, but that peaks in atrazine concentrations were of longer duration than those of glyphosate. The 2-day sampling also indicated that weekly sampling is unlikely to capture peak concentrations of glyphosate and atrazine.

Bifenthrin Causes Trophic Cascade and Altered Insect Emergence in Mesocosms: Implications for Small Streams.

Direct and indirect ecological effects of the widely used insecticide bifenthrin on stream ecosystems are largely unknown. To investigate such effects, a manipulative experiment was conducted in stream mesocosms that were colonized by aquatic insect communities and exposed to bifenthrin-contaminated sediment; implications for natural streams were interpreted through comparison of mesocosm results to a survey of 100 Midwestern streams, USA. In the mesocosm experiment, direct effects of bifenthrin exposure included reduced larval macroinvertebrate abundance, richness, and biomass at concentrations (EC50's ranged from 197.6 to 233.5 ng bifenthrin/g organic carbon) previously thought safe for aquatic life. Indirect effects included a trophic cascade in which periphyton abundance increased after macroinvertebrate scrapers decreased. Adult emergence dynamics and corresponding terrestrial subsidies were altered at all bifenthrin concentrations tested. Extrapolating these results to the Midwestern stream assessment suggests pervasive ecological effects, with altered emergence dynamics likely in 40% of streams and a trophic cascade in 7% of streams. This study provides new evidence that a common pyrethroid might alter aquatic and terrestrial ecosystem function at the regional scale.

A comparative study of the modeled effects of atrazine on aquatic plant communities in midwestern streams.

Potential effects of atrazine on the nontarget aquatic plants characteristic of lower-order streams in the Midwestern United States were previously assessed using the Comprehensive Aquatic System Model (CASMATZ ). Another similar bioenergetics-based, mechanistic model, AQUATOX, was examined in the present study, with 3 objectives: 1) to develop an AQUATOX model simulation similar to the CASMATZ model reference simulation in describing temporal patterns of biomass production by modeled plant populations, 2) to examine the implications of the different approaches used by the models in deriving plant community-based levels of concern (LOCs) for atrazine, and 3) to determine the feasibility of implementing alternative ecological models to assess ecological impacts of atrazine on lower-order Midwestern streams. The results of the present comparative modeling study demonstrated that a similar reference simulation to that from the CASMATZ model could be developed using the AQUATOX model. It was also determined that development of LOCs and identification of streams with exposures in excess of the LOCs were feasible with the AQUATOX model. Compared with the CASMATZ model results, however, the AQUATOX model consistently produced higher estimates of LOCs and generated non-monotonic variations of atrazine effects with increasing exposures. The results of the present study suggest an opportunity for harmonizing the treatments of toxicity and toxicity parameter estimation in the CASMATZ and the AQUATOX models. Both models appear useful in characterizing the potential impacts of atrazine on nontarget aquatic plant populations in lower-order Midwestern streams. The present model comparison also suggests that, with appropriate parameterization, these process-based models can be used to assess the potential effects of other xenobiotics on stream ecosystems.

Quantification of uncertainty in estimated nitrate-N loads in agricultural watersheds

Summary Estimation of annual nutrient loads is critical for many applications in water quality management; however, measurement errors and infrequent monitoring could raise considerable uncertainty in the load estimations. The objectives of this study were to quantify the overall uncertainty in annual nitrate-N load estimates. Nitrate was selected as the study object because of the high loads common in Midwestern streams, and its important influence on hypoxia in the Gulf of Mexico. A standard error propagation method was used to quantify the uncertainty from both measurement and load calculation processes to evaluate the accumulative effect. The measurement uncertainty was assessed based on standard information; the load calculation uncertainty was evaluated through numerical simulation. The study found that uncertainty in annual nitrate-N load estimates for a 30-day sampling frequency, expressed as coefficient of variation (CV), was 14–34%. This included measurement (flow and concentration) uncertainty of 12% and load calculation uncertainty of 7–32%. These results can be used to evaluate different uncertainty sources, estimate uncertainty for similar watersheds, and design sampling programs balancing cost and accuracy.

Stability Analysis of Semicohesive Streambanks with CONCEPTS : Coupling Field and Laboratory Investigations to Quantify the Onset of Fluvial Erosion and Mass Failure

AbstractThe overarching goal of this study is to perform a comprehensive bank stability analysis that is phenomenologically sound by considering both mass failure and fluvial erosion. The nature of this study is twofold. First, field and experimental analyses are conducted to generate data for channel cross-section properties, soil index properties, and mechanical and erosional strengths at two sites in a representative, midsize, midwestern stream in southeastern Iowa that is subjected to frequent flash floods and characterized by active fluvial erosion and cantilever failure. Second, the channel surveys and data obtained from the field and laboratory analyses are used as input parameters for an established one-dimensional, channel evolution model, namely, the conservational channel evolution and pollutant transport system (CONCEPTS, version 2.0, Langendoen and Alonso 2008), to estimate the factor of safety for mass failure (FSm) and fluvial erosion (FSf) and simulate the bank retreat as a result of eithe...

Modeling the potential effects of atrazine on aquatic communities in midwestern streams

The comprehensive aquatic systems model for atrazine (CASM(ATZ)) estimates the potential toxic effects of atrazine on populations of aquatic plants and consumers in a generic lower-order midwestern stream. The CASM(ATZ) simulates the daily production of 20 periphyton and 6 aquatic vascular plant species. The modeled consumer community consists of 17 functionally defined species of zooplankton, benthic invertebrates, bacteria, and fish. Daily values of population biomass (grams of carbon per square meter) are calculated as nonlinear functions of population bioenergetics, physical-chemical environmental parameters, grazing/predator-prey interactions, and population-specific direct and indirect responses to atrazine. The CASM(ATZ) uses Monte Carlo methods to characterize the implications of phenotypic variability, environmental variability, and uncertainty associated with atrazine toxicity data in estimating the potential impacts of time-varying atrazine exposures on population biomass and community structure. Comparisons of modeled biomass values for plants and consumers with published data indicate that the generic reference simulation realistically describes ecological production in lower-order midwestern streams. Probabilistic assessments were conducted using the CASM(ATZ) to evaluate potential modeled changes in plant community structure resulting from measured atrazine exposure profiles in 3 midwestern US streams representing watersheds highly vulnerable to runoff. Deviation in the median values of maximum 30-d average Steinhaus similarity index ranged from 0.09% to 2.52% from the reference simulation. The CASM(ATZ) could therefore be used for the purposes of risk assessment by comparison of site monitoring-based model output to a biologically relevant Steinhaus similarity index level of concern. Used as a generic screening technology or in site-specific applications, the CASM(AT) provides an effective, coherent, and transparent modeling framework for assessing ecological risks posed by pesticides in lower-order streams.

Assessment of quantitative food web metrics for investigating the influence of land use on warm water fish diets

Lotic systems in many regions of the country have experienced habitat degradation and biodiversity loss due to agricultural activity and urbanization. Southeastern Michigan is no exception, as agriculture in the River Raisin watershed and increased urbanization in the Huron River watershed threatens both systems. To further understand the ecological impact of land use on trophic interactions in Midwestern streams and assess the use of a selected set of weighted, quantitative food web metrics as a tool for investigating the influence of anthropogenic disturbance on these systems we compared summer food webs for nine second-order streams. All streams were categorized as developed, undeveloped, or agricultural based on land cover data. Developed and undeveloped streams were located in the Huron River watershed and agricultural streams were located in the River Raisin watershed. Reach-level habitat quality was also assessed at each study site using the EPA’s Rapid Habitat Assessment. Fish diets (n = 410) were analyzed to create summer food webs for each site. Comparisons of food webs were made using a suite of weighted, quantitative metrics to identify differences in fish–macroinvertebrate interactions across streams with differing land cover at the sub-basin scale and habitat quality at the local scale. Although undeveloped streams had higher species richness and less habitat degradation, no significant patterns were observed in the quantitative metrics across the three stream categories or based on reach-level habitat conditions. Decapoda, terrestrial Hymenoptera, and Chironomidae were the primary prey taxa in all stream categories. Decapods accounted for the majority of biomass consumed and the pattern of this consumption strongly influenced metric scores. The suite of quantitative metrics tested in this study did not detect significant differences in fish–macroinvertebrate food webs across land use categories, likely in part due to the dominance of a large, tolerant prey taxa in fish diets, regardless of land use and local habitat quality.

Environmental controls of wood entrapment in upper Midwestern streams

AbstractWood deposited in streams provides a wide variety of ecosystem functions, including enhancing habitat for key species in stream food webs, increasing geomorphic and hydraulic heterogeneity and retaining organic matter. Given the strong role that wood plays in streams, factors that influence wood inputs, retention and transport are critical to stream ecology. Wood entrapment, the process of wood coming to rest after being swept downstream at least 10 m, is poorly understood, yet important for predicting stream function and success of restoration efforts. Data on entrapment were collected for a wide range of natural wood pieces (n = 344), stream geomorphology and hydraulic conditions in nine streams along the north shore of Lake Superior in Minnesota. Locations of pieces were determined in summer 2007 and again following an overbank stormflow event in fall 2007. The ratio of piece length to effective stream width (length ratio) and the weight of the piece were important in a multiple logistic regression model that explained 25% of the variance in wood entrapment. Entrapment remains difficult to predict in natural streams, and often may simply occur wherever wood pieces are located when high water recedes. However, this study can inform stream modifications to discourage entrapment at road crossings or other infrastructure by applying the model formula to estimate the effective width required to pass particular wood pieces. Conversely, these results could also be used to determine conditions (e.g. pre‐existing large, stable pieces) that encourage entrapment where wood is valued for ecological functions. Copyright © 2010 John Wiley &amp; Sons, Ltd.

Fish passage over hydraulic structures in Midwestern Rivers of the USA

The main objective of this study was to examine the hydraulic performance of knickpoint‐control structures (i.e., riprap weirs, grouted‐riprap weirs, baffled and non‐baffled fish ladders) to facilitate unimpeded fish passage. The hydraulic performance of these structures, located in Midwestern streams, was evaluated by determining the mean and turbulent flow characteristics in the vicinity of the structures via detailed field tests. Non‐intrusive mean flow measurements were performed using a 3‐dimensional sidelooking Flowtracker handheld Acoustic Doppler Velocimeter (ADV) and by utilizing the newly developed Large Scale Particle Image Velocimetry (LSPIV) technique. Non‐intrusive turbulent flow measurements were performed using a field ADV with a high sampling frequency. Results from the mean flow measurements illustrated that the mean flow depth (Y) and velocity (V) atop the structures were critical for evaluating the performance of the structures. In addition, the present study suggested that the (Y, V) should be viewed in conjunction with the drainage area (DA), slope (S) of the structure, and structure type. The DA size affected (Y, V) in the streams, S affected the power of the water action, and the structure type could cause flow acceleration/deceleration. Results from the turbulent flow measurements illustrated that the flow approaching the structures became 3‐dimensonal, while the size of eddies in the baffled fish ladder were found to disorient fish in the transverse direction due to sudden constriction. In short, the results presented herein illustrated that a complete assessment of the hydraulic performance of structures should include not only the conventional permissible criteria for (Y, V) but also the turbulent flow characteristics at the locations atop the structures.

Nitrification and denitrification in a midwestern stream containing high nitrate: in situ assessment using tracers in dome-shaped incubation chambers

The extent to which in-stream processes alter or remove nutrient loads in agriculturally impacted streams is critically important to watershed function and the delivery of those loads to coastal waters. In this study, patch-scale rates of in-stream benthic processes were determined using large volume, open-bottom benthic incubation chambers in a nitrate-rich, first to third order stream draining an area dominated by tile-drained row-crop fields. The chambers were fitted with sampling/mixing ports, a volume compensation bladder, and porewater samplers. Incubations were conducted with added tracers (NaBr and either 15N[NO3−], 15N[NO2−], or 15N[NH4+]) for 24–44 h intervals and reaction rates were determined from changes in concentrations and isotopic compositions of nitrate, nitrite, ammonium and nitrogen gas. Overall, nitrate loss rates (220–3,560 μmol N m−2 h−1) greatly exceeded corresponding denitrification rates (34–212 μmol N m−2 h−1) and both of these rates were correlated with nitrate concentrations (90–1,330 μM), which could be readily manipulated with addition experiments. Chamber estimates closely matched whole-stream rates of denitrification and nitrate loss using 15N. Chamber incubations with acetylene indicated that coupled nitrification/denitrification was not a major source of N2 production at ambient nitrate concentrations (175 μM), but acetylene was not effective for assessing denitrification at higher nitrate concentrations (1,330 μM). Ammonium uptake rates greatly exceeded nitrification rates, which were relatively low even with added ammonium (3.5 μmol N m−2 h−1), though incubations with nitrite demonstrated that oxidation to nitrate exceeded reduction to nitrogen gas in the surface sediments by fivefold to tenfold. The chamber results confirmed earlier studies that denitrification was a substantial nitrate sink in this stream, but they also indicated that dissolved inorganic nitrogen (DIN) turnover rates greatly exceeded the rates of permanent nitrogen removal via denitrification.

Atrazine and increased male production by Daphnia: The importance of combining field and laboratory approaches

Atrazine is one of the most commonly applied herbicides in North America and annually pulses through many midwestern stream and reservoir systems. Previous studies have yielded conflicting results regarding the ability of atrazine to stimulate male production by Daphnia, an effect hypothesized to lower population growth rates during a period of intense larval fish predation. In the present study, populations of Daphnia parvula and Daphnia ambigua exhibited high proportions of males but no ephippial females when atrazine pulsed into Acton Lake, a small midwestern reservoir. Field results thus supported the hypothesis of excess male production by Daphnia during the spring herbicide pulse. In laboratory studies, dose-response studies, and population-level assays revealed no effect of atrazine on male production or population growth rate of multiple clones differing in reproductive strategy and exposure history. However, D. parvula increased male production in response to an endogenous crustacean hormone (methyl farnesoate). Excess male production observed in the field population was therefore not likely caused by atrazine, although we cannot rule out the possibility of interactive effects of atrazine and some other stressor. Apparent signs of endocrine disruption in the presence of high concentrations of a suspected agent should be viewed with caution in the absence of parallel laboratory studies involving individuals from the populations of interest.