Middle Life Research Articles

Effects of Target of Rapamycin and Phosphatidylinositol 3-Kinase Inhibitors and Other Autophagy-Related Supplements on Life Span in y w Male Drosophila melanogaster

Various dietary supplements have been shown to extend the life span of Drosophila melanogaster, including several that promote autophagy, such as rapamycin and spermidine. The goal of the study presented here was to test numerous additional potential anti-aging supplements, primarily inhibitors of the target of rapamycin (TOR) and/or phosphatidylinositol 3-kinase (PI3K). Using a single, comparatively long-lived y w test strain, screening was performed in male flies supplemented either throughout adulthood or, in a few cases, beginning in middle or late adult life, with concentrations spanning 4–6 orders of magnitude in most cases. Supplementation with PP242 and deferiprone, an iron chelator, beginning in late adult life had no positive effect on life span. Lifelong supplementation with Ku-0063794, LY294002, PX-866-17OH, Torin2 and WYE-28 had no effect at any dose. Rapamycin, spermidine and wortmannin all had significant life-shortening effects at the highest doses tested. AZD8055, PI-103 hydrochloride and WYE-132 yielded slight beneficial effects at 1–2 doses, but only 100 nM AZD8055 was confirmed to have a minor (1.3%) effect in a replicate experiment, which was encompassed by other control groups within the same study. These compounds had no effect on fly fecundity (egg laying) or fertility (development of progeny to adulthood), but equivalent high doses of rapamycin abolished fertility. The solvent DMSO had no significant effect on life span at the concentrations used to solubilize most compounds in the fly medium, but it drastically curtailed both survival and fertility at higher concentrations. 2-Hydroxypropyl-β-cyclodextrin also failed to extend the life span when provided throughout adulthood or beginning in mid-adult life. Collectively, the results suggest that inhibition of the TOR/PI3K pathway and autophagy through dietary intervention is not a straightforward anti-aging strategy in Drosophila and that further extension of life is difficult in comparatively long-lived flies.

The Influence of Parental Psychological Control on Life Satisfaction of Middle School Students: The Mediating Role of Parent-Child Relationship and the Regulatory Role of Sensation-Seeking

Based on self-determination theory and ecosystem theory, this study examines the mediating role of parent-child relationship between parental psychological control and middle school student life satisfaction, as well as the moderating effect of sensation seeking. The study uses a questionnaire survey method to predict the relationship between variables. The results indicate that: (1) Parental psychological control has a significant negative predictive effect on parent-child relationships and middle school student life satisfaction; (2) The parent-child relationship partially mediates the relationship between parental psychological control and middle school student life satisfaction; (3) The relationship between parental psychological control and life satisfaction in middle school students seeking trait regulation.

Development of a Home Economics Education Program for the Consumer Life Area to Enhance Artificial Intelligence Literacy in Middle School Students

This study aims to develop and validate a home economics education program focused on the consumer life area to enhance artificial intelligence (AI) literacy. To achieve this objective, a 10-session AI-consumer life integration education program was developed by analyzing literature on AI Literacy, AI curriculum, consumer education, and the home economics curriculum for the middle school consumer life area. The program’s validity was assessed by nine teachers using a four-point Likert scale. The average scores for each item and the content validity index (CVI) were calculated. Based on expert feedback, the program was revised and improved accordingly. The expert validity assessment of the lesson plans, teaching materials and learning resources resulted in an average score of 3.78 for all items and an average CVI of 0.96. For the overall program, the expert validity assessment yielded an average score of 3.72 for all items and an average CVI of 0.97. Since the content validity index for all questions was above 0.78, the program demonstrated high validity across achievement standards integration, learning objectives, content and teaching methods, motivation, and volume areas. This confirms its effectiveness as an educational program for enhancing AI literacy. This study is significant in terms of defining and identifying the components of AI literacy, developing an AI-integrated program encompassing the entire consumer life area and confirming the suitability of the home economics curriculum for enhancing digital consumer competencies and promoting sustainable consumption. Additionally, it highlights the potential to integrate AI into the home economics and consumer life area.

Association Between Adverse Early Life Factors and Telomere Length in Middle and Late Life.

Telomere length (TL) has been acknowledged as biomarker of biological aging. Numerous investigations have examined associations between individual early life factors and leukocyte TL; however, the findings were far from consistent. We evaluated the relationship between individual and combined early life factors and leukocytes TL in middle and late life using data from the UK Biobank. The early life factors (eg, maternal smoking, breastfeeding, birth weight, and comparative body size and height to peers at age 10) were measured. The regression coefficients (β) and 95% confidence interval (CI) were applied to assess the link of the early life factors and TL in adulthood. Flexible parametric survival models incorporated age to calculate the relationship between early life factors and life expectancy. Exposure to maternal smoking, lack of breastfeeding, low birth weight, and shorter height compared to peers at age 10 were identified to be associated with shorter TL in middle and older age according to the large population-based study with 197 504 participants. Individuals who experienced more than 3 adverse early life factors had the shortest TL in middle and late life (β = -0.053; 95% CI = -0.069 to -0.038; p < .0001), as well as an average of 0.54 years of life loss at the age of 45 and 0.49 years of life loss at the age of 60, compared to those who were not exposed to any early life risk factors. Early life factors including maternal smoking, non-breastfed, low birth weight, and shorter height compared to peers at age 10 were associated with shorter TL in later life. In addition, an increased number of the aforementioned factors was associated with a greater likelihood of shorter TL in adulthood, as well as a reduced life expectancy.

Total life expectancy and disability-free life expectancy and differences attributable to cigarettes’ smoking among Chinese middle-aged and older adults

ObjectivesMiddle-aged and older adults smoking for years are afflicted by smoking-related diseases and functional limitations; however, little is known about the effect of smoking on nonfatal conditions in middle and later life. This study aims to investigate the impact of smoking on both total life expectancy (TLE) and disability-free life expectancy (DFLE) and the variations in such effects by educational level in China.MethodsData were drawn from the China Health and Retirement Longitudinal Study (CHARLS), 2011–2018, with a total sample of 16,859 individuals aged 45 years or older involved in the final analysis. The Activities of Daily Living (ADL) scale was used to measure disability, and the population-based multistate life table method was used to estimate the differences in TLE and DFLE by smoking status and educational attainment.ResultsAt baseline, 28.9% of participants were current smokers, 8.5% were former smokers, and 62.6% never smoked. Approximately 5.6% were identified with ADL disability. Both current smokers and former smokers experienced lower TLE and DFLE than never smokers, and such differences were particularly prominent among men. Intriguingly, former smokers manifested a lower DFLE for both sexes and a lower TLE among women, though a longer TLE among men, compared with current smokers. Similar differences in TLE and DFLE by smoking status were observed for groups with different levels of education.ConclusionNever smokers live longer and healthier than current smokers and persons who quit smoking. Smoking was associated with greater reductions in TLE and DFLE among men. However, educational attainment might not moderate the adverse effect of smoking on both fatal and nonfatal conditions in the context of China. These findings have implications for disability prevention, aged care provision and informing policies of healthy aging for China and elsewhere.

An examination of younger and older adults' age preferences.

Research suggests that how people feel about aging can contribute to their later physical, cognitive, and mental health. In two studies, we examined younger (ages 18-30) and older adults' (ages 61-70) views about aging by asking them to rate the extent to which they would find it desirable to be various ages between 0 and 120. Participants also indicated both their ideal age (the age at which they would most like to be) and their subjective age (how old they generally feel). Consistent with the previous studies, younger adults' ideal age was significantly younger than older adults' ideal age. Younger adults' subjective age was slightly older than their chronological age, whereas older adults' subjective age was slightly younger than their chronological age. Of interest was the finding that, for older adults, their desirability ratings of various ages gradually decreased after age 35. In contrast, younger adults' desirability ratings decreased precipitously after age 20 and remained low from age 45 through age 90. Results suggest that older adults view middle to later life in a more nuanced manner than younger adults, who viewed middle and later life as being undesirable and homogenous. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Parental Alcohol Exposures Associate with Lasting Mitochondrial Dysfunction and Accelerated Aging in a Mouse Model.

Although detrimental changes in mitochondrial morphology and function are widely described symptoms of fetal alcohol exposure, no studies have followed these mitochondrial deficits into adult life or determined if they predispose individuals with fetal alcohol spectrum disorders (FASDs) to accelerated biological aging. Here, we used a multiplex preclinical mouse model to compare markers of cellular senescence and age-related outcomes induced by maternal, paternal, and dual-parental alcohol exposures. We find that even in middle life (postnatal day 300), the adult offspring of alcohol-exposed parents exhibited significant increases in markers of stress-induced premature cellular senescence in the brain and liver, including an upregulation of cell cycle inhibitory proteins and increased senescence-associated β-galactosidase activity. Strikingly, in the male offspring, we observe an interaction between maternal and paternal alcohol use, with histological indicators of accelerated age-related liver disease in the dual-parental offspring exceeding those induced by either maternal or paternal alcohol use alone. Our studies indicate that chronic parental alcohol use causes enduring mitochondrial dysfunction in offspring, resulting in a reduced NAD+/NAHD ratio and altered expression of the NAD+-dependent deacetylases SIRT1 and SIRT3. These observations suggest that some aspects of FASDs may be linked to accelerated aging due to programmed changes in the regulation of mitochondrial function and cellular bioenergetics.

Economic Impact Assessment of Structural Health Monitoring Systems on the Lifecycle of a Helicopter Blade

Structural Health Monitoring Systems (SHMS) are currently widely investigated in the literature, however, their application to the aerospace industry is still limited. One of the reasons lies in the lack of methods aimed at evaluating their economic impact on the lifecycle of the structural element to be monitored [1]. In this work the economic impact of FBG-based SHMS was evaluated on a composite helicopter rotor blade, considering two perspectives: Beginning Of Life (BOL), concerning the blade production, and Middle Of Life (MOL), concerning its usage. The processes were modeled using the IDEF0 methodology, which allowed deriving the cost models for two scenarios: the current one, and the one including the SHMS. In BOL, the SHMS was supposed providing support to the development of new composite rotor blades, including the curing cycle definition and certification tests. In MOL the SHMS was supposed performing automatic scheduled inspections coupled with the traditional ones. The cost model was implemented in the Probabilistic Damage Tolerance Analysis [2] to compare the scenarios having the same blade Probability Of Failure and considering the performances of the SHMS in terms of Probability Of Detection and Probability of False Alarm. Results show that an economic benefit may be achieved using the SHMS in the development of new blades, potentially reducing the number of blades tested and number of autoclave cycles. Moreover, it was found that the traditional scheduled detailed inspection intervals could be extended if automatic SHMS inspections are performed in addition, maintaining the same Probability Of Failure. [1] Büchter, K. D. et al. (2022). Modeling of an aircraft structural health monitoring sensor network for operational impact assessment. Structural Health Monitoring, 21(1), 208-224. [2] Lin, K. Y., &amp; Styuart, A. V. (2007). Probabilistic approach to damage tolerance design of aircraft composite structures. Journal of Aircraft, 44(4), 1309-1317.

Cast no shadow: a phenomenological study of a middle child

Each family member has unique talents, and a solid family unit is built on the bond between siblings and parents. Middle children's lives have historically cast a shadow and been ignored in the family since older siblings are recognized for how they become the people they become. In comparison, younger siblings are recognized for their accomplishments. This study aims to explore the lived experiences of middle children, specifically their struggles, coping mechanisms, and insights. This study utilized a phenomenological qualitative research design; the selected participants were eight middle children of the family. Through thematic analysis using Colaizzi's method, this study gathered all the data from the participants. The study results showed the following themes: unfair treatment in the family as a struggle, relationships between middle children and their peers, and independence as a coping mechanism, while existential validation of the middle children as their insight. This research provides valuable insights that middle children need recognition and appreciation by accepting their unique perspectives and essential role in society and their lives.

Interaction between maternal immune activation and postpartum immune stress in neuropsychiatric phenotypes

Epidemiological evidence has shown that maternal infection is a notable risk factor for developmental psychiatric disorders. Animal models have corroborated this link and demonstrated that maternal immune activation (MIA) induces long-term behavioural deficits and neuroimmunological responses to subsequent immune stress in offspring. However, it is unclear whether MIA offspring are more sensitive or more tolerant to immunological challenges from postnatal infections. Pregnant mice were weighed and injected with a single dose of polyinosinic-polycytidylic acid (poly I:C) or saline at gestational day 9.5, and their male offspring were exposed to poly I:C or saline again during adolescence, adulthood, and middle life. After a two-week recovery from the last exposure to poly I:C, the mice underwent behavioural and neuroendophenotypic evaluations. Finally, the mice were sacrificed, and the expression levels of inflammatory factors and the activation levels of glial cells in the cerebral cortex and hippocampus were evaluated. We found MIA mice have lifelong behavioural deficits and glial activation abnormalities. Postpartum infection exposure at different ages has different consequences. Adolescent and middle life exposure prevents sensorimotor gating deficiency, but adult exposure leads to increased sensitivity to MK-801. Moreover, MIA imposed a lasting impact on the neuroimmune profile, resulting in an enhanced cytokine-associated response and diminished microglial reactivity to postnatal infection. Our results reveal an intricate interplay between prenatal and postpartum infection in neuropsychiatric phenotypes, which identify potential windows where preventive or mitigating measures could be applied.

Short communication: Lifetime musical activity and resting-state functional connectivity in cognitive networks.

Participation in multimodal leisure activities, such as playing a musical instrument, may be protective against brain aging and dementia in older adults (OA). Potential neuroprotective correlates underlying musical activity remain unclear. This cross-sectional study investigated the association between lifetime musical activity and resting-state functional connectivity (RSFC) in three higher-order brain networks: the Default Mode, Fronto-Parietal, and Salience networks. We assessed 130 cognitively unimpaired participants (≥ 60 years) from the baseline cohort of the DZNE-Longitudinal Cognitive Impairment and Dementia Study (DELCODE) study. Lifetime musical activity was operationalized by the self-reported participation in musical instrument playing across early, middle, and late life stages using the Lifetime of Experiences Questionnaire (LEQ). Participants who reported musical activity during all life stages (n = 65) were compared to controls who were matched on demographic and reserve characteristics (including education, intelligence, socioeconomic status, self-reported physical activity, age, and sex) and never played a musical instrument (n = 65) in local (seed-to-voxel) and global (within-network and between-network) RSFC patterns using pre-specified network seeds. Older participants with lifetime musical activity showed significantly higher local RSFC between the medial prefrontal cortex (Default Mode Network seed) and temporal as well as frontal regions, namely the right temporal pole and the right precentral gyrus extending into the superior frontal gyrus, compared to matched controls. There were no significant group differences in global RSFC within or between the three networks. We show that playing a musical instrument during life relates to higher RSFC of the medial prefrontal cortex with distant brain regions involved in higher-order cognitive and motor processes. Preserved or enhanced functional connectivity could potentially contribute to better brain health and resilience in OA with a history in musical activity. German Clinical Trials Register (DRKS00007966, 04/05/2015).

Analysis of the Antioxidant Mechanism of Ozone Treatment to Extend the Shelf Life and Storage Quality of ‘Korla’ Fragrant Pears Based on Label-Free Proteomics

In this study, the physiological indicators, reactive oxygen species (ROS) levels, and activities and expressions of key enzymes related to ROS metabolism were monitored to explore the mechanism of ozone treatment on the shelf life of postharvest Korla fragrant pears. The results show that postharvest fragrant pears treated with ozone had a higher firmness and lower weight loss rate and decay rate during their shelf life, especially in the late stage. Ozone treatment could also delay the occurrence of the respiratory peak and reduce the peak value. The generation rate of superoxide anion (O2−), the hydrogen peroxide (H2O2) content, and the malondialdehyde (MDA) level were reduced in the ozone-treated group, while the activities of key enzymes, superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD), related to ROS metabolism in postharvest fragrant pears were stimulated by ozone treatment, especially in the middle and late stages of shelf life. Analysis of the proteomics results indicates that the POD family enzymes were the main target protein in postharvest fragrant pears treated by ozone during the middle and late stages of shelf life. The activity and expression of antioxidant-related enzymes in postharvest fragrant pears were stimulated by ozone to accelerate the metabolism of ROS and maintain high quality, especially in the middle and late shelf lives.

Gender disparities and potentials in STEM approach in Jordan and Saudi Arabia – An analytical literature review

AbstractIn recent years, the integrated approach of STEM disciplines (Science, Technology, Engineering, and Mathematics) has been adopted in the Middle East to improve students' scientific capacities and their formative thinking. Nevertheless, this approach encounters complications in the application, including many due to gender differences. Middle Eastern women's life and education is affected by conservative constraints and social norms where gender stereotypes and culture impact shared views about specific domains. Research regarding gender has frequently emphasized gender imbalance in virtually all STEM study fields and professions. This study explores STEM education's conceptual framework in Jordan and Saudi Arabia. Likewise, it provides an overview of STEM teachers' practices and their gender perspectives in the classrooms. We investigate the implementations and gender differences in STEM education by scrutinizing relevant literature and studies in the selected countries. The conclusions indicate a shortage of teachers' knowledge in applying STEM education in classrooms and a need for more development programs that qualify teachers for STEM education applications. In addition, the results show that gender disparities are promoted by the education system and teachers who teach STEM subjects in schools because social norms and gender stereotypes influence them.

Unveiling Cultural Identities and Self Savouring in Preethi Nair’s One Hundred Shades of White and Sarah Addison Allen’s The Girl Who Chased the Moon

Literature often deals with the notion of self-discovery. Self-realisation is the pinnacle of the inner journey that characters undergo in literature. Different individuals experience it at various stages; some in their adolescence, some in middle life, while others in their later years. Savouring is the act of taking pleasure in and appreciating something, frequently food but also other pursuits like music, nature, and relationships. The ability to savour something correlates with enhanced happiness, health, and contentment in life. Savouring serves as a method of introspection. Discovering oneself, one's ideals, and what provides delight and purpose in life aid in cherishing unique and varied experiences. Thus, this paper attempts to provide a comparative study of two novels, One Hundred Shades of White by Preethi Nair and The Girl Who Chased the Moon by Sarah Addison Allen, in which the female protagonists try to savour their self-discovery through various means, namely food, culture, and identity.

Life-course social participation and physical activity in midlife: longitudinal associations in the 1970 British Cohort Study (BCS70)

A hypothesized benefit of social participation is that it encourages people to be more physically active. However, limited evidence exists on the association between social participation over the life-course and physical activity in midlife. We sought to apply a life-course framework to examine the association of social participation and device measured physical activity in midlife in the UK. We used the 1970 British Birth Cohort Study (BCS70), which includes all people born in Britain during a single week in 1970. Social participation was assessed at ages 16, 30, 34 and 42. Physical activity was measured by accelerometery at age 46, as mean daily step count and time spent in moderate to vigorous physical activity (MVPA). The associations of social participation and physical activity were tested using two different life-course models: the sensitive period model and the accumulation model. Individuals with medium and high participation compared to no social participation over their life-course had higher mean daily step count and MVPA in midlife, supporting the accumulation model. In the sensitive period model, only those that actively participated at age 42 had higher mean daily steps and MVPA compared to those who did not participate. Our study provides empirical evidence on the importance of sustaining social participation at all ages over the life-course rather than at a particular timepoint of someone’s life. If our findings reflect causal effects, interventions to promote social participation throughout the life-course could be an avenue to promote physical activity in middle life.

Logistics Service Provider Lifecycle Model in Industry 4.0: A Review

Supply chain and logistics management is of tremendous importance for multinational organizations. Logistics Service Providers (LSPs) provide logistics services and smooth logistics operations between suppliers, manufacturers, distributors, and customers. This paper uses a Systematic Literature Review (SLR) to identify the current trends and future developments of LSPs and the underlying (smart) logistics operations connected to the concept of lifecycle management. An SLR review was conducted to identify relevant research papers in the areas of LSPs and logistics lifecycle management. Out of 288 papers analyzed, 81 were identified as highly appropriate for in-depth analysis. The LSP Lifecycle Model (LSLM) was then developed by combining logistics service characteristics and the lifecycle management concept, including Product Lifecycle Management (PLM), Service Lifecycle Management (SLM), and Product Service System (PSS). The LSLM consists of three phases: The Beginning of Life (BOL), the Middle of Life (MOL), and the End of Life (EOL). The LSLM is characterized by three phases, eight criteria, and seventeen sub-criteria. This paper aims to fulfil customer requirements through a product or service in the whole lifecycle of the logistics service provider. The findings further present an adaptable LSLM by focusing on various logistics services and integrating sustainability factors to meet market trends. Logistics cost factors can also be used to evaluate logistics services in the MOL stage. The EOL shows the trend of risk management, evaluation, and decomposition, which is determined by new or re-designed logistics products and services.

Examining the Determinants of Poland’s Middle Class Life Satisfaction

Recent works suggest that the increasing income inequalities decreased the middle class size and threatened life satisfaction (LS). However, no such studies link LS with middle-income class problems. The present study aims to fill this gap and characterize LS across the middle-income class in Poland. The main novelties are the Satisfaction with Life Scale (SWLS) to measure LS of the middle-income class in Poland and the methodological approach based on classification-regression trees (CART) to identify the determinants of LS. The analysis is conducted on the new database obtained from the survey of members of the middle-income class in Poland divided into three subgroups: the lower-middle-income class, the mid-income middle class, and the higher-middle-income class. For comparison, the low-income and high-income classes were also considered. Based on the SWLS results, relatively low diversity in middle-income class LS was identified. However, the inter-class comparison revealed that LS increased with belonging to a higher income class. The CART results support the primary importance of economic conditions such as, for instance, wealth and the level of savings in determining the middle-income class LS and show that their effect depends on their characteristics like professional status.

The impact of adverse childhood experiences on depression in middle and late life: A national longitudinal study

BackgroundAdverse childhood experiences (ACEs) are one of the causes of depression in middle-aged and older adults, but the combined effects of ACEs, cognitive function and ability to perform activities of daily living (ADL) on depression have not been fully explored. MethodsThis study was based on data from 4 waves (2013, 2014, 2015 and 2018) of data from the China Health and Retirement Longitudinal Study, including 10,995 middle-aged and older adults. ACEs were derived from the 2014 self-report life history module. A latent variable growth curve model was used to assess the mediating effect of cognitive function and ability to perform ADL in the relationship between ACEs and depression. ResultsACEs were significantly associated with lower initial cognitive status (β = −0.156, P < .001), worse ability to perform ADL (β = 0.051, P < .001) and higher severity of depression (β = 0.228, P < .001). The results of mediation analysis indicated that the association between ACEs and the intercept of depression was partly mediated by the initial level of cognitive function and ADL, and the association between ACEs and the slope of depression was total mediated by cognitive (intercept and slope) and ADL (intercept and slope). ConclusionsACEs were associated with higher severity of depression in part due to lower cognitive function and worse ability to perform ADL. Interventions that focus on reducing ACEs and improving cognitive level and ability to perform ADL may effectively reduce the incidence of depression among middle-aged and older individuals.

Multistate survival modelling of multimorbidity and transitions across health needs states and death in an ageing population

BackgroundUnmet health needs have the potential to capture health inequality. Nevertheless, the course of healthcare needs fulfilment, and the role of multimorbidity in this process remains unclear. This study assessed...

Life-Course Socioeconomic Position and Mild Cognitive Impairment in Midlife: Evidence from the 1958 British Birth Cohort

BackgroundDementia has been the leading cause of death in the UK since 2015. Increasing evidence supports an association between socioeconomic position (SEP) and dementia onset in later life. However, limited studies have examined how life-course SEP influences the development of mild cognitive impairment (MCI), an intermediate state potentially preceding dementia. Therefore, the present study aims to examine the relationship between life-course SEP and MCI amongst adults aged 50 years in Great Britain.MethodsWe employed data from the National Child Development Study (NCDS), also known as the 1958 British Birth Cohort, to determine the associations between SEP and MCI in 6590 participants. We categorised life-course measures of SEP as stable high/low or moving upward/downward over the life course. We assessed MCI at age 50 using one standard deviation below the averaged combined scores from all cognitive tests available. We then used binary logistic regression to estimate the longitudinal associations between life-course SEP and MCI.ResultsRelative to those of a high SEP across the life course, participants who moved upward, downward, or remained at a low SEP were significantly associated with 25% (95% CI 1.02–1.54, p = 0.035), 70% (95% CI 1.27–2.27, p < 0.001), and 85% (95% CI 1.50–2.29, p < 0.001), respectively, higher odds of MCI, independent of all selected covariates.ConclusionsLower life-course SEP was associated with significantly higher odds of MCI onset in middle life within the NCDS cohort. Public health policies targeting cognitive impairment should encompass a life-course approach to reduce socioeconomic inequalities.