The study was conducted to examine the cytotoxicity of Ficus benjamina against Human Embryonic (HEK293T) cell lines. The leaves of Ficus benjamina were obtained and washed under running water, shade-dried to constant weight and ground into fine pounder, and extracted using chloroform. The chloroform extract was further separated into samples A, B, C, D, and E with the use of Thin Layer Chromatography (TLC). HEK293T cells were cultivated in DMEM or Dulbecco's Modified Eagle's Medium. The cells were then seeded at a density of 8 x 104 cells/mL in 96-well tissue culture plates, resulting in 4000 cells in 50 L of the medium in each well. With the use of the CellTitre-Glo 2.0 reagent, cytotoxicity was evaluated. In each well containing 50 liters of media, cells were treated by adding 50 liters of fresh media with a 2X concentration of the test sample and 2% DMSO, resulting in a final concentration of the test sample with 1% DMSO. After that, the treated cells were incubated for 48 hours at 37°C with 5% CO2. The luminescent signal generated was measured using a microplate luminometer with a 500 ms integration time per well.