Microliters Of Antiserum Research Articles

Immunologic Protection against VX Intoxication in Experimental Animals

Rabbits immunized with an artificial VX-antigen could survive 1 x LD95 of VX challenge on the 7th day and the 31st day after the last immunization. One hundred microliters of rabbit anti-VX antiserum given intravenously immediately before 1 x LD95 of VX or 200 microliters of antiserum intraperitoneally 1-10 days before 1 x LD95 VX protected all the tested mice from death. The antiserum could prevent the in vitro inhibition of Torpedo AChE activity by VX and reduced its effect on brain AChE activity in vivo. No protective effect of the antiserum was observed on the Torpedo AChE activity inhibited by sarin and soman.

Use of protein A-positive Staphylococci as adsorbent in a radioimmunoassay for cyclic AMP and cyclic GMP

A reproducible (SD less than 4%), sensitive (in the 5 to 50 fmoles range) and inexpensive radioimmunoassay has been set up for the quantitation of cyclic. AMP and cyclic GMP based on acetylation or succinylation of the test sample. Separation of antibody-bound from free ligand was achieved by adsorption to formalinized protein A-positive Staphylococci of the Cowan 1 strain. The quantity of adsorbent (5% suspension) needed per 300 microliters of antiserum (diluted 24 x 10(4)) was 10 microliters. The blank value was below 2% and separation could be run at room temperature or at 4 degrees C as convenient. The acetylation or succinylation procedure of the sample totally eliminated interference of test sample immunoglobulins with antiserum binding to the absorbent.

Resistance changes in lipid bilayers: immunological applications.

The electrical resistance of a bimolecular lipid membrane in 0.1 molar NaCl decreases if antibody and complement are present on one side of the membrane and the homologous antigen is added to the other side. The reaction occurs within minutes and requires less than 0.1 microliter of antiserum.