Microleakage Of Fissure Sealants Research Articles

Clinical Evaluation and Microleakage of Fissure Sealant: Effect of Laser Etching vs Acid Etching

Aim: The aim of this study was to evaluate the clinical success and the sealing ability of a resin based fissure sealant that applied enamel surface which was etched with conventional phosphoric acid and Er:YAG laser at different energy levels. Material and Methods: The study consisted of two parts; the evaluation of retention, marginal discoloration, marginal integrity and new caries formation for the clinical success after the treatment at 3, 6, 12 months; the evaluation of microleakage and penetration depth to fissures using dye penetration test. The in vivo part of the study was the surface preparation processes, 72 teeth in each group; It consists of 32% phosphoric Acid, Er:YAG laser- MSP (Medium Short Pulse) and Er:YAG laser- QSP (Quantum Square Pulse). A total of 45 impacted human third molar teeth were used for in vitro part. Data were analyzed using SPSS 21. Results: The relationship between the success rates of 12-month clinical follow-up of fissure sealants and age, gender, and localization was assessed and there was no statistically significant correlation. When all criteria were considered, the most successful group was QSP. All the groups demonstrated microleakage regardless of the surface preparation techniques. However, there was no statistically significant difference between the groups. Conclusion: It is considered to determine the optimal energy level of laser will increase the clinical success.

Comparison of Retention and Marginal Discoloration of Self-Etch and Total-Etch Adhesives before Pit and Fissure Sealant Application on Permanent Second Molars in Pediatric Patients. A 1-year follow-up longitudinal study

Objective: To compare the retention and marginal discoloration of Self-etch and Total Etch Adhesive during Pit and Fissure Sealant application at 1 month, 3 months, 6 months, and 12 months. Materials and methods: 30 school-going children received sealants in all four permanent second molars, carried out in the Department of Pedodontics and Preventive Dentistry. The fully erupted second permanent molars were randomly allocated into 3 groups: group A (self-etch), B (Total etch), and C (control group). According to the split mouth design, each patient received sealant in the second molar with self-etch in one arch and total-etch in the other arch; contra-lateral molars were kept in the control group. Results: No statistically significant difference was found in the marginal discoloration and microleakage of fissure sealants placed on occlusal surfaces following use of an SE bonding agent and traditional TE adhesive after 12 months. Conclusion: When TE adhesive was put on before sealant, the same amount of microleakage or marginal discoloration happened in the occlusal fissure of permanent teeth as when SE adhesive was put on before sealant.

Effects of tooth preparation on the microleakage of fissure sealant

Background: Fissure sealing can be achieved by preparing and sealing the deep pits and fissures in the teeth with a sealant to prevent caries. Fissure sealing is performed using resin modified glass ionomer cement (RMGIC) and failure is most often due to weak adhesion between the material and the tooth, resulting in microleakage. Purpose: The study aimed to determine the effect of a preparation technique with bur and acid application on potential RMGIC fissure sealant microleakage. Methods: Twenty-four extracted maxillary premolars were divided into four treatment groups. Group 1 underwent enameloplasty with a round bur and application of 37% phosphoric acid; group 2 with a tapered bur and 37% phosphoric acid; group 3 with a round bur and 10% polyacrylic acid; and group 4 with a tapered bur and 10% polyacrylic acid. The application of 37% phosphoric acid was carried out for 15 seconds, while 10% polyacrylic acid was applied for 20 seconds, before RMGIC filling. The teeth were stored in artificial saliva at 37°C for 24 hours, then thermocycled 100 times at 5°C and 55°C for 20 seconds each. The teeth were immersed in a 1% methylene blue solution for 24 hours at 37°C, then cut crosswise. The length of the microleakage was observed with a stereo microscope at 8 times magnification and measured using raster image application. Data was analysed with one-way ANOVA. Results: Significant differences were found between treatment groups (F=562.14; p<0.05). The deepest mean microleakage was in the round bur and 10% polyacrylic acid group (1657.87 ± 78.08) and the shallowest was in the round bur and 37% phosphoric acid group (500.70 ± 38.55). Conclusion: The preparation method, type of bur and acid solution have an effect on microleakage potential of RMGIC fissure sealing. Round bur preparation and 37% phosphoric acid resulted in shallow microleakage.

The Effect of Enamel Pre-treatment with Silver Diamine Fluoride versus Nano Silver Fluoride on the Microleakage of Fissure Sealant: in vitro study.

Abstract: Many studies in literature showed the effectiveness of silver diamine fluoride (SDF) and Nano silver fluoride (NSF) as preventive agents. However, there is little evidence regarding the retention properties of fissure sealant (FS) when applied on a surface previously treated with these preventive agents. Objective: To evaluate and compare the effect of fissure pre-treatment with SDF versus NSF on the microleakage of FS in permanent teeth. Methods: This experimental in vitro study was performed on 45 extracted human premolars, teeth were randomly assigned into three groups according to the type of fissure treatment: Group I: Control group (sealant only), Group II: SDF group (pre-treatment before sealant), and Group III: NSF group (pre-treatment before sealant). Teeth were thermos-cycled and then immersed in 2% methylene blue solution for 24 hours, teeth were then sectioned mesio-distally in the middle of the fissure, and examined under the stereomicroscope for dye penetration. Results: There was no statistical significant difference in the microleakage scores between the three groups (p=0.247). Conclusion: Pretreatment of the pits and fissure with SDF or NSF prior to sealant application did not affect the micro-leakage of the FS.

Assessment of shear bond strength and microleakage of fissure sealant following enamel deproteinization: An in vitro study.

BackgroundTo evaluate the effect of two different deproteinizing agents (5.25% sodium hypochlorite and Papacarie®) prior to acid etching on the shear bond strength and microleakage of a fissure sealant material. Material and MethodsA total of 123 extracted noncarious human third molar teeth were randomly divided into three groups for each test: acid etching alone (control) (C), sodium hypochlorite (NaOCl), and Papacarie Duo® (PC). The shear bond strength tests were performed using a universal testing machine, and microleakage was evaluated using a dye penetration method. ResultsThe mean shear bond strength values obtained in Group C were significantly lower than those of Group NaOCl and Group PC (p=0.0001). There were no significant differences between Group NaOCl and Group PC (p>0.05). No statistically significant difference was observed among the groups in terms of microleakage (p>0.05). ConclusionsPapacarie Duo® and 5.25% sodium hypochlorite treatments before etching increased the shear bond strength of the fissure sealant. However, pre-treatment with these agents did not decrease the microleakage between teeth and sealant. Key words:Deproteinization, microleakage, papain, dental seal, shear bond strength.

Effect of Dabbing Motion During Etching on Microleakage of Fissure Sealants: An In vitro Study

Effect of Dabbing Motion During Etching on Microleakage of Fissure Sealants: An In vitro Study

Effect of Precuring and Postcuring of Total-Etch and Self-Etch Bonding Agents on the Microleakage of Fissure Sealants.

Objectives:Considering the importance of timesaving in pediatric dentistry, if the efficacy is achieved along with shorter working time and less technical sensitivity, the behavior management of young patients can be anticipated. This study aimed to compare the effect of precuring and postcuring of total-etch and self-etch bonding agents on the microleakage of sealants.Materials and Methods:This study was conducted on forty impacted third molars, which were surgically extracted. The samples were divided into five groups: 1. Control (etching and sealant), 2. Precured fifth-generation bonding agent (Adper Single Bond 2) and fissure sealant, 3. Postcured fifth-generation bonding agent (Adper Single Bond 2) and fissure sealant, 4. Precured seventh-generation bonding agent (Single Bond Universal) and fissure sealant, and 5. Postcured seventh-generation bonding agent (Single Bond Universal) and fissure sealant. All specimens were thermocycled (×1000), immersed in 0.5% basic fuchsine solution for 24 hours, sectioned, digitally photographed, and measured using the LAS EZ program. The data were analyzed using Kruskal-Wallis and Dunn’s tests.Results:Leakage in the control group and the third group was significantly lower than that in other groups. In pairwise comparisons, a significant difference was found between the control group and the fifth group and between the third group and the fifth group.Conclusion:The conventional method of sealant placement showed superior results in comparison with the use of an intermediate layer of the bonding agent.

A comparison of microleakage in self-etch fissure sealants and conventional fissure sealants with total-etch or self-etch adhesive systems.

Objective:The present study was conducted to compare microleakage in self-etching fissure sealants and conventional fissure sealants with total-etch or self-etch adhesive systems.Settings and Design:This experimental in vitro study was conducted on 60 healthy third molars extracted from humans. The first group received Acid etch + Clinpro sealant, the second group received Acid etch + Single bond 2 + Clinpro sealant, the third group received Single bond universal (self-etching bonding) + Clinpro sealant, and the fourth group received prevent seal self-etching sealant.Materials and Methods:An incision was made on the teeth after they were immersed in methylene blue 5%. The samples were then examined under a stereomicroscope and the dye penetration rate was measured based on the Williams and Winter criteria.Statistical Analysis Used:The Kruskal–Wallis and Mann–Whitney tests were used for data analysis in SPSS-18 (P < 0.05).Results:Group 1 which was treated with the conventional technique (acid + fissure sealant) had the highest rate of microleakage compared to Groups 2 and 3 (P < 0.001).Conclusion:The results showed that the use of bonding results in a significant reduction in the microleakage of fissure sealants. The microleakage caused when using self-etch fissure sealant was not different from that caused by the use of the conventional method.

Effects of sealant, viscosity, and bonding agents on microleakage of fissure sealants: An in vitro study.

Objective:The aim of this study was to evaluate the effects of enamel or dentin bonding agent (DBA) and sealant viscosity on sealant microleakage.Materials and Methods:Sixty extracted human premolars were randomly divided into two equal groups (based on sealant viscosity) and each group was divided into three subgroups of 10 teeth. Group 1 (low viscosity sealant, Seal-Rite, Pulpdent, USA with 7.7% filler): Prophylaxis, enameloplasty, etching of occlusal surfaces with 38% of phosphoric acid gel, rinsing and drying, followed by (1) enamel bonding agent (EBA) (Margin Bond, Coltène/Whaledent AG) or (2) DBA (Excite, Ivoclar Vivadent AG, Liechtenstein) or (3) no bonding (NB) prior to sealant application. In Group 2, similar procedures were performed except for applying a high viscosity sealant (Seal-Rite, Pulpdent, The USA with 34.4% filler). Specimens were thermocycled and then immersed in a 0.5% basic fuchsine solution for 24 h next, buccolingual slices of samples were scored under a stereomicroscope. The Kruskal–Wallis and Mann–Whitney U-tests were used for data analysis.Results:There was no significant difference between DBA, EBA, and NB subgroups in the microleakage scores in both groups. Low viscosity sealant had a lower microleakage than the high viscosity sealant in both DBA (P = 0.002) and NB (P = 0.041) subgroups.Conclusion:The results indicated that the use of low viscosity sealant reduced the microleakage of pit and fissure sealants. However, the use of a bonding agent before sealant placement didn’t affect the microleakage.

Effect of Different Enamel Preparation Methods on Microleakage of Fissure Sealant: An In Vitro Study

Objective: Lack of a dental material with optimal adhesion and sealability is an important challenge in modern dentistry leading to marginal leakage. There are controversies on the necessity of enamel preparation in pit and fissure sealant therapy and its effect on decreasing the microleakage; therefore, the present in vitro study aimed to assess the amount of microleakage with and without enamel preparation. Methods: In this experimental study, 30 sound premolars assigned suitable for sealant application, were chosen and randomly divided into two groups. Sealant was applied to all teeth with the same conventional technique. In group A, fissure sealant was applied without enamel preparation while in group B, sealant was applied after fissurotomy with bur. The teeth were thermocycled and microleakage was measured using silver nitrate as leakage tracer. The teeth were then cut into three bucco-lingual sections and examined under a stereomicroscope with 32× magnification. The amount of dye penetration into the sealant was recorded in microns and the degree of microleakage was classified into four degrees of 0, 1, 2 and 3. T-test was applied for the comparison of data between the two groups. Results: In total,20% of specimens in group B (fissurotomy) had degree 1 and 80% had degree 0microleakage and no specimen had degrees 2 and 3 microleakage, while in group A (no preparation), 20% had degree 1, 33.3% had degree 2 and 46.7% had degree 3microleakage. No specimen had degree 0 microleakage. Therefore, placement of sealant with enamel preparation significantly decreased microleakage (p<0.001). Conclusion: In view of the findings of this investigation, it seems that enamel preparation reduces marginal leakage in pit and fissure sealant therapy.

Enamel pretreatment with Er:YAG laser: effects on the microleakage of fissure sealant in fluorosed teeth.

ObjectivesThe purpose of this in vitro study was to evaluate the microleakage and penetration of fissure sealant in permanent molar teeth with fluorosis after pretreatment of the occlusal surface.Materials and MethodsA total of 120 third molars with mild dental fluorosis were randomly divided into 6 groups (n = 20). The tooth surfaces were sealed with an unfilled resin fissure sealant (FS) material. The experimental groups included: 1) phosphoric acid etching (AE) + FS (control); 2) AE + One-Step Plus (OS, Bisco) + FS; 3) bur + AE + FS; 4) bur + AE + OS + FS; 5) Er:YAG laser + AE + FS; and 6) Er:YAG laser + AE + OS + FS. After thermocycling, the teeth were immersed in 0.5% fuchsin and sectioned. Proportions of mircoleakage (PM) and unfilled area (PUA) were measured by digital microscope.ResultsOverall, there were significant differences among all groups in the PM (p = 0.00). Group 3 showed the greatest PM, and was significantly different from groups 2 to 6 (p < 0.05). Group 6 showed the lowest PM. Pretreatment with Er:YAG with or without adhesive led to less PM than bur pretreatment. There were no significant differences among groups in PUA.ConclusionsConventional acid etching provided a similar degree of occlusal seal in teeth with fluorosis compared to those pretreated with a bur or Er:YAG laser. Pretreatment of pits and fissures with Er:YAG in teeth with fluorosis may be an alternative method before fissure sealant application.

The effect of different enamel surface treatments on microleakage of fissure sealants

The aim of this study was to evaluate the effects of different techniques of surface treatment on the microleakage of fissure sealants in permanent molar teeth in vitro. 96 extracted impacted human third molars were randomly divided into 8 surface treatment groups (n=12/group) as 1. Er: YAG laser ; (Fidelis II, Fotona, Ljubljana, Slovenia) (125 mj, 20Hz) ; 2. Er: YAG laser + 37% H3PO4 (15s) ; 3. ER: AG laser + 37% H3PO4+Prime&Bond NT ; 4. Er: YAG laser + G Bond ; 5. Er: YAG laser + Prime&Bond NT ; 6. 37% H3PO4 ; 7. 37% H3PO4 + Prime&Bond NT ; 8. G Bond. Sealant material (Clinpro, 3M ESPE, Seefeld, Germany), was applied into the fissures and light-cured for 20s with LED (Bluephase C5, Ivoclar-Vivadent, Schaan, Liechtenstein). Specimens were subjected to thermocycling (1000×, 5-55°C, dwell time: 15s) and immersed in 0.5% basic fuchsin solution for 24h at 37°C. The samples were sectioned and scored on a 3 point rating scale using a light microscope with a magnification of ×20. One-way analysis of variance was used to analyze data. Multiple comparisons were analyzed using Bonferroni test (p=0.05). Er:YAG laser showed the highest microleakage scores whereas Er YAG laser + 37% H3PO4 showed the lowest. Although 37% H3PO4 group showed higher scores than Er:YAG laser + 37% H3PO4, the difference was not statistically significant. Etching fissures with phosphoric acid is sufficient prior to fissure sealant application.

The effect of different enamel surface treatments on the microleakage of fissure sealants

The aim of this study was to evaluate the effects of different techniques of surface treatment on the microleakage of a fissure sealant in molar teeth. A total of 50 freshly extracted noncarious human third molars were randomly assigned to one of five groups. Occlusal fissures were treated with one of the following: acid etching with 35% orthophosphoric acid (group 1); fissurotomy with a Fissurotomy Micro NTF metal bur (group 2); laser etching with an Er,Cr:YSGG laser at 2 W and 20 Hz (group 3); laser etching with an Er,Cr:YSGG laser at 2 W and 40 Hz (group 4); and air abrasion for 20 s with 30-µm Al(2)O(3) particles via a CoJet Prep device (group 5). After surface pretreatment, a resin-based sealant was applied to the fissures. The sample teeth were subjected to thermocycling and stored in distilled water at 37°C for 1 month. Following immersion in 0.5% basic fuchsin solution for 24 h, three buccolingual slices of each sample tooth were scored under a stereomicroscope, and the morphological appearance of the area between the enamel surface and fissure sealant was examined under a scanning electron microscope. The Kruskal-Wallis test and one-way ANOVA revealed significant differences in marginal leakage, as follows: group 1 showed significantly lower scores than groups 2 and 5, the scores of groups 1, 3 and 4 were not significantly different, and group 2 showed significantly higher scores than groups 3 and 4. Laser irradiation, the metal bur, and the CoJet Prep device did not eliminate the need for acid etching of the enamel prior to placement of a fissure sealant. Laser etching at 2 W (20 Hz or 40 Hz) may be an alternative to conventional acid-etching.

Analysis of surface roughness and microleakage of fissure sealants following organic debris removal with Carisolv

Objective The objective of the present study was to improve fissure sealing by pre-treatment with Carisolv in order to remove organic debris. The surface morphology and roughness of fissure cavities and the degree of microleakage after Carisolv application were compared with those after bristle brush treatment in vitro. Methods Fifty extracted human teeth were used in this study. The teeth were randomly divided into two groups of 25 each. Artificial fissures were prepared in all teeth into which artificial organic debris was placed. The debris in 25 teeth of one group was removed using Carisolv applied for 30 s and excavation was performed with a dental explorer until the gel was clear. The remaining 25 teeth were then cleaned using bristle brush with prophylaxis paste. Surface roughness was analyzed in five samples from each group by color laser three-dimensional (3D) microscopy and by scanning electron microscopic examination. The remaining samples were filled with sealant and subjected to a microleakage test under thermocycling. Statistical analysis was performed using the Mann–Whitney U-test; a value of p < 0.05 was considered significant. Results Morphologically, most of the debris in the fissures was removed by Carisolv treatment, whereas some fissures were not cleaned by bristle brush. Carisolv-treatment with acid-etching resulted in removal of debris-like smear layer leaving enamel prisms open, and 3D laser microscopy demonstrated that the roughness values increased after acid-etching. However, microleakage test of both Carisolv and brush methods showed similar results. Conclusions Carisolv-treated surfaces especially when subjected to acid-etching might facilitate good adaptation of sealant to enamel, because of an increase in surface roughness and favorable surface characteristics.