The identification of bacteria is central to the microbiological sciences. While gene sequencing methods have been the standard to identify bacteria, use of MALDI-TOF mass spectrometry (MS) in clinical microbiology provides high-throughput identification to the subspecies level. However, biotyping has yet to be adopted outside of clinical settings due to the lack of a centralized public database of MS protein signatures that would facilitate strain identification via spectral comparison. Herein we present the IDBac web platform, a crowd-sourced central knowledgebase of protein MS signatures of >1400 strains spanning 6 bacterial phyla. Accompanying the knowledgebase is analysis infrastructure to identify unknown isolates, probe relationships within culture collections, and visualize specialized metabolite differences within groups of closely related bacteria. We highlight this utility by demonstrating the dereplication of bacterial isolates using the seed knowledgebase, identifying trends in culture collections using metadata integration, and reporting the discovery of a new metabolite from a Paraburkholderia isolate.

Background: Vibrio, a group of Gram‑negative bacteria found in the ocean, has become a significant global threat, intensified by climate change, owing to its crucial roles in environmental, human, and animal health. Research on these bacteria and the diseases they cause has greatly influenced scientific progress, resulting in major advancements in the fields of microbiology, epidemiology, and public health. Objectives: This review aims to highlight the early groundbreaking discoveries in Vibrio research, particularly those that have significantly impacted the science of microbiology and global health. Methods: A comprehensive literature search was conducted across vast databases of biomedical and life sciences literature including PubMed, EMBASE, and Scopus. Additionally, a search of the grey literature was performed. Studies that marked early groundbreaking discoveries in Vibrio research, with wide implications for human society, were included. Findings and conclusion: Research on Vibrio has led to major advancements in our understanding of disease mechanisms, pathogen ecology, and the epidemiology of waterborne infections. A landmark discovery was the identification of Vibrio cholerae in 1884, which played a crucial role in studying waterborne diseases such as cholera and led to the development of modern approaches to treat diarrheal diseases, such as the introduction of oral rehydration salt (ORS) therapy. Certain Vibrio strains, such as Vibrio vulnificus, are important models for studying flesh‑eating diseases, while others, such as Vibrio parahaemolyticus ST3, ST36, and V. cholerae O1, are the only marine bacteria known to cause global epidemics by spreading across continents. Key mechanisms in Gram‑negative bacteria, including the viable but nonculturable (VBNC) state, quorum sensing, and the type VI secretion system (T6SS), were first discovered in Vibrio species. Today, research on Vibrio bacteria remains crucial from a global health perspective, especially owing to the expanding effects of climate change on their worldwide distribution.

Enhancing environmental decontamination and sustainable production through synergistic and complementary interactions of actinobacteria and fungi.

BackgroundReported are results of SPECTRA data from MexicoMethodsSPECTRA (n=617) is a 7-country multicentre retrospective observational study, including hospitalized adult patients (≥18 years) treated with C/T for ≥48 hours. Medical records were reviewed for 30 days after the last dose of C/T or until death. Reported are clinical outcomes and treatment patterns in Mexico.Table 2.All-cause in-hospital mortality (ACHM) in the Mexico cohortResultsMean age of patients (n=59) is 55.2 years, and 47.5% were female. Common comorbidities were diabetes mellitus (DM) uncomplicated (18.6%) and DM with end organ damage (3.4%), transplantation (16.9%), heart disease (16.9%), chronic kidney disease (11.9%), chronic pulmonary disease (10.2%), acute kidney injury (10.2%), and end-stage renal disease (3.4%). From positive cultures (n=32) were found 21 multi-drug resistant (MDR) Pseudomonas aeruginosa (PsA) (65.6%) and 4 MDR Enterbacterecea (12.5%). C/T was used to treat pneumonia (30/59, 50.8%), complicated intra-abdominal infection (9/59,15.3%), complicated urinary tract infection (8/59, 13.6%), bone and joint infection (8/59, 13.6%), and sepsis (8/59, 13.6%). Mean time from the index hospitalization admission to C/T initiation is 16.0 days. Mean time from the first microbiology (MB) sample for the index infection to C/T initiation is 1.9 days. C/T treatment duration was median 8.0 days (Q1:Q3; 5, 11). Doses included 54.2% 1.5g/Q8h; 39.0% 3g/Q8h; 5.1% 750mg/Q8h; 1.7% 1g/Q8h. C/T rank of initiations: first-line (49.2%), second-line (42.4%), third-line (6.8%), fourth-line (0), fifth-line (1.7%), sixth+line (0). Of all the cases, empiric therapy accounted for (37/59) 62.7%, definitive therapy in (17/59) 28.8%, and undetermined (5/59) (8.5%).Clinical success rate for treating the index infection with C/T is 79.7% of patients (Table 1). All-Cause-Hospital-Mortality (ACHM) is 16.9%. Median time from index date to death is 12.0 days (Table 2). ICU admission is reported in 42.4% of cases, with a median ICU length of stay at 20.0 days (Table 3).Table 3.ICU admission and LOS during the index hospitalisation from the Mexico† ICU LOS if ICU admission was related to index infection.CI, confidence interval; ICU, intensive care unit; LOS, length of stay; SD, standard deviation.ConclusionResults show real-world effectiveness of C/T in Mexico, more research is warranted.DisclosuresYanbing Zhou, PhD, Merck: I am a full time Merck Employee and own stocks in the retirement plan provided by Merck.|Merck: Stocks/Bonds (Public Company) Thales Polis, MD, MSD: Stocks/Bonds (Public Company) Emre Yucel, PhD, Merck: I am a full time Merck Employee and own stocks in the retirement plan provided by Merck.|Merck: Stocks/Bonds (Public Company)

To assess whether dental colleges provide education on effectively managing infectious diseases within the role of dentists as primary care providers for infectious diseases such as COVID-19, a total of 11 domestic dental colleges were examined. The aim was to evaluate whether their curricula included education on managing infectious diseases including severe postvaccination adverse reactions and the legal aspects pertinent to infectious diseases.Through the analysis of detailed items, eight categories were selected: Immunology, Microbiology, Pathology, Pharmacology, Clinical Assessments and Procedures, Side Effects and Emergencies, Infection Control, and Health Care-related Laws. All 11 domestic dental colleges provided sufficient education in the four basic sciences of Immunology, Microbiology, Pathology, and Pharmacology, as well as training on Infection Control and Health Care-related Laws. However, the category of ‘Side Effects and Emergencies’ had the lowest implementation rate, at 80%. A coordinated effort to establish consensus on the required competencies for infectious disease management is needed. Furthermore, a deeper understanding of the educational content related to ‘Side Effects and Emergencies’ is necessary.

Abstract For more than four thousand years technological advances have enabled us to see the invisible through the invention and development of microscopes that have delivered magnification alongside resolution. Over the same timescale this has led to the discovery of microscopic organisms and the birth of the science of microbiology. From ancient theories that an invisible life form existed light microscopy established their presence in the 1600s. Over the next couple of centuries light microscopes were developed with improved illumination and precision as microbiologists created ways to grow and sample their microbes. In so doing microscopy and microbiology extended our understanding of infection transmission from generalised miasma and germ theories to linking specific diseases with particular microbes to make diagnosis and prevention more reliable. Electron and fluorescent microscopy provided step changes in the visualisation of the finer detail of microbes, enabling the characterisation of internal structures and the unravelling of the molecular mechanisms of biological processes. We are now within an era of imaging microbes in three dimensions over time with sensitivity and super resolution to study the microbes in their natural habitat such as within infected tissues. With the advent of multi-modal, correlative and molecular imaging the future holds the promise of a full interrogation of the internal machineries of microbes and real-time tracking of their lifecycle and interactions. This will help deliver the next generation of antibiotics and alternative prevention strategies to enable everyone to live longer, healthier lives.

Aims Spondylodiscitis (SpD), a debilitating infective condition of the spine, mandates early diagnosis and institution of appropriate therapy, for which accurate microbiology and histological evaluation of the affected tissue is vital. The objectives of the study were to assess the correlation between clinical and magnetic resonance imaging (MRI) findings with histopathology (HPE) and microbiology (MB) in clinically diagnosed spondylodiscitis. Settings and design This was a prospective study of 34 consecutive patients reporting at the outpatient department of a tertiary hospital with clinical and imaging features of SpD, who underwent image-guided/surgical biopsy of lesions. Methods and material The provisional diagnosis of SpD in all patients was made on the combined basis of clinical profile and MRI Spine findings. Tissue samples in all patients, obtained by either open surgery or CT-guided biopsy, were subjected to HPE and MB analysis. Results SpD has a bimodal age distribution with the majority of patients being males in the fourth to fifth decades. Only raised erythrocyte sedimentation rate (ESR) was consistently seen amongst laboratory parameters, with leucocytosis being added pointer towards pyogenic etiology. MRI remained the imaging modality of choice for SpD but was not dependable for etiologic differentiation. On HPE and MB evaluations, 24 patients (71%) had findings consistent with infective SpD, while combined results augmented etiologic confirmation for 28 patients (82.4%). HPE was more sensitive than traditional MB methods to determine etiology in SpD, but the addition of the GeneXpert (Cepheid,Sunnyvale, California, United States) technique improved the MB positivity rate, especially in patients with tubercular SpD. Six patients (17.6%) with both negative HPE and MB results were categorized as 'Non-specific' SpD. Conclusions SpD poses a challenge to determine the etiology for the administration of specific antimicrobial therapy. A stratified standard institutional approach needs adoption to systematically evaluate SpD patients by having a high index of clinical suspicion, early imaging, followed by tissue biopsy for HPE and MB. Despite efforts to reach a diagnosis, a subset of patients without conclusive etiologic agent identification would remain as 'Non-specific', needing empiric antibiotic treatment based on clinico-radiologic profile.

Background: Food is imperative for continuation of life. However, it is also an important vehicle of entry of infections. Bacterial, viral and parasitic agents, causing these infections can all spread by food. Bacteria can produce many toxins and also be invasive sometimes, which can lead to diarrhoea and dysentery, respectively. This contamination takes place by risks like cooking food at improper temperature and keeping food open after cooking. These risks lead to various hazards. Also, microbes can help prepare different foods like fermented foods and kombucha tea. Modern society relies heavily on processed and ready-to eat foods, both of which can cause foodborne infections. Keeping all these things in mind, the science of food microbiology becomes very important in modern times. Aim: All these aspects of food microbiology and food safety have been discussed in this chapter. Objectives: Many points like food safety, cleanliness and other aspects like chilling of cooked food minimize risks of microbial food contamination and resultant hazards, have been elaborated upon. Methods: Scientific literature search was carried out to study the risk factors and related reports with respect to food microbiology, by food scientists and others. Results: Food contamination can be of microbial origin and a multitude of factors may lead to microbial contamination of food. These factors could be improper cooking, leaving cooked food uncovered, and other things. Conclusion: Food microbiology is a very important aspect of public health and quite neglected too. It should be given its due importance to mitigate microbial contamination of food and consequent foodborne infections.

Lessons learned from the successful polio vaccine experience not learned or applied with the development and implementation of the COVID-19 vaccines.

ABSTRACT We designed a microbial science popularization product based on virtual reality technology, “Micro World – Exploring the Microbial Kingdom,” and conducted usability tests. Participants used head-mounted VR glasses to experience virtual scenarios teaching microbiological knowledge. The product’s user experience was evaluated through a User Experience Questionnaire (UEQ), and the results showed that our product provided both usefulness and enjoyment. In addition, the quality of participants’ relevant knowledge acquisition was measured using the Student Education of Educational Quality (SEEQ). The SEEQ results show that “Micro World” is an effective tool for popularizing microbiology science and can actively promote students’ independent learning and improve learning quality.

The WGO Guideline "Endoscope Disinfection" is intended for use by health providers and professionals who are involved in the use, cleaning, and maintenance of endoscopes and aims to support national societies, official bodies and individual endoscopy departments in developing local standards and protocols for reprocessing endoscopes. This updated Endoscope Disinfection Guideline addresses the recent outbreaks of multi-drug resistant organisms after endoscopy and proposes measures to reduce the risks of these outbreaks occurring. The recommendations are based on the consensus findings of an international multidisciplinary working group with expertise in microbiology, including biofilms, endoscope reprocessing, nursing, and gastroenterology, and with broad experience in developing national and international reprocessing guidelines. - First Name Country 1. Tony Speer (Chair) Australia 2. Michelle Alfa Canada 3. Alistair Cowen Australia 4. Dianne Jones Australia 5. Karen Vickery Australia 6. Helen Griffiths UK 7. Roque Sáenz Chile 8. Anton LeMair Netherlands GUIDELINES OR STANDARDS Reprocessing instructions are often called guidelines but are, in fact, a technical standard that sets out the minimum acceptable practice for reprocessing to deliver high-level disinfection of endoscopes. The distinction between the 2 terms is important. Medical guidelines usually address a narrow clinical question using population-based data, often results of randomized trials in a specific population, to guide the care of an individual patient.1 Standards are broader in the application and set out specifications and procedures designed to ensure products, services, and systems are safe, reliable, and consistently perform the way they were intended. The supporting evidence for a standard is based on science, technology, and experience rather than clinical trials. The standards governing reprocessing are based on the science of cleaning, disinfection, drying, and microbiology, and recommendations are supported by measurements of efficacy in models with artificial soils and/or a known inoculum of bacteria. The implementation of the appropriate standards for reprocessing should follow the general principles of good manufacturing practice (GMP). GMP is a set of regulations, codes, and guidelines for a manufacturing process, in this case reprocessing an endoscope to produce a high-level disinfected endoscope. These regulations cover both performances of reprocessing and quality control of the process. GMP is recognized worldwide for the control and management of manufacturing and quality control testing of pharmaceutical products and has evolved over the last 60 years in response to multiple well-publicized problems in the pharmaceutical industry.2 While the terms guidelines and standards are both used to describe instructions for endoscope reprocessing3,4 these instructions are best considered as a technical standard. General Principles in Endoscope Reprocessing The most important step in endoscope reprocessing is scrupulous manual cleaning before disinfection. Disinfection will fail if the cleaning has been inadequate.5–7 Manual cleaning must be undertaken by a person familiar with the structure of the endoscope and trained in cleaning techniques. Cleaning should begin immediately after the endoscope is used so that biological material does not dry and harden. Appropriate detergents and cleaning equipment should be used; in particular appropriate diameter brushes should be used for each channel. Cleaning should be followed by thorough rinsing to ensure all debris and detergents are removed before disinfection. Manual Cleaning Pre-Cleaning—Immediately after each procedure, with the endoscope still attached to the light source, the insertion tube should be wiped with a lint-free disposable cloth and the distal tip placed in a low foaming medical grade detergent solution and detergent aspirated through all channels, including the suction/biopsy channel. The air/water channels should be flushed with detergent, and then all channels flushed, including the jet channel if present, with water, then air, as per the manufacturer's instructions. A specific valve may be required to flush the air/water channels with detergent. The endoscope should be removed from the light source and transported to the cleaning area in a closed container that avoids environmental contamination from drip or spill, and that clearly indicates that the endoscope within is contaminated. It is essential that the endoscope is not allowed to dry before further cleaning as this will make the removal of organic matter difficult or impossible. Endoscopes should be processed without delay within 30 minutes. Leak testing should be performed to check the integrity of all channels before further processing. All the valves and buttons should be removed and leak testing performed as per the manufacturer's instructions. Buttons and valves should be brushed and disinfected, paying particular attention to internal surfaces and high-level disinfection or sterilization according to the original equipment manufacturer's instructions. The endoscope should be placed in a detergent solution in a sink in the "dirty" section of the decontamination area and the outer surface washed. A low foaming medical grade detergent should be used at the appropriate dilution according to the manufacturer's instructions. All accessible sections of the suction biopsy channel should be brushed according to the manufacturer's instructions for use, and each channel should be brushed until all debris is removed. The tip and handles and clean valve seats should be brushed and then cleaning adapters fitted and channels flushed with fresh detergent for the product specified time. The endoscope should be rinsed by draining the detergent from the sink, rinsing the outer surface with cold running tap water, then filling the sink with tap water and purging the channels with tap water using the cleaning adapters following the manufacturer's instructions. The channels should be purged with air to remove rinse water. Disinfection High-level disinfection is performed in an Automatic Flexible Endoscope Reprocessor (AFER) that should comply with the relevant national standard or be approved by the Food and Drug Administration (FDA). The AFER may or may not have an automated cleaning and disinfection cycle. All connectors should be specifically designed for each endoscope model. All channels should be connected at the start and end of a cycle. The detachable components, including the air/water and suction valves, can be steam sterilized or reprocessed with the endoscope if the ability of the AFER to clean and/or disinfect these detachable components is validated by the AFER manufacturer. After high-level disinfection, the endoscope is rinsed in the AFER with bacteria-free water produced by sub-micron filters. Water quality should be checked regularly. Manual high-level disinfection is another option that is effective when performed by well-trained, dedicated reprocessing staff supplied with appropriate personal protective equipment. The endoscope should be immersed in disinfectant, and all channels are filled with disinfectant solution, and the buttons and valves should be immersed in the disinfectant. The instrument should be soaked for the required time at the required temperature and concentration as specified by the disinfectant manufacturer. All channels should be purged with air to remove the disinfectant, the exterior of the endoscope rinsed and the channels flushed with bacteria-free water, with the volume required for the specific disinfectant used, to remove any traces of disinfectant. Drying Endoscopes should be dried after each procedure by purging the water from the channels with compressed air, then flushing the channels with alcohol, followed by forced air drying. Alcohol flush facilitates drying and is a useful adjunct to disinfection because of its bactericidal effects.8 The use of alcohol may not be permitted in some countries (France, UK) because of concerns about variant Creutzfeldt-Jakob disease. The endoscope should be stored in a forced air-drying cabinet to supplement drying. If an endoscope is used infrequently, it is reasonable to store it separately hanging vertically in a purpose-built cabinet as opposed to a forced air storage/drying cabinet, and then reprocess the endoscope before the next patient use. Endoscopes should be dried completely before hanging. Accessories The water bottle should be changed after each endoscopy session and steam sterilized. The water bottle should be filled with sterile water immediately before use. Documentation All essential steps of endoscope reprocessing should be documented for quality assurance and for patient tracing if necessary. OUTBREAKS The recent reports of outbreaks of multi-drug resistant organisms (MDROs) after endoscopy, particularly carbapenem-producing Enterobacteriacae (CPE), have focused critical attention on the efficacy and safety of reprocessing protocols. CPE has become established in the hospital environment and may cause clinical infections with substantial morbidity and mortality because of their antibiotic resistance. Outbreaks of CPE after endoscopy have been reported in several countries often after ERCP9 but also following bronchoscopy,10 gastroscopy,11–13, and flexible cystoscopy.14,15 Often, microbiological surveillance identifies a single source for an outbreak of MDROs that can be traced to a culprit endoscope that has transmitted genetically similar bacteria on multiple occasions despite reprocessing. MDROs may also be transmitted sporadically by endoscopes without a single source being identified by genetic studies. In case-control studies of hospital inpatients, a recent endoscopy, including gastroscopy, bronchoscopy, and ERCP, was a significant risk factor for acquiring MDRO colonization/infection.13,16–18. The risk of transmitting infection at endoscopy is underestimated; a risk of 1 in 1.8 million is often quoted.19 The correlation of an infection with a previous endoscopy is difficult to establish confidently, and if established, is not always reported to authorities and is seldom published.20,21 The paucity of reports from less developed countries is likely to be due to failure of detection and reporting rather than a true absence. Cultures of patient-ready endoscopes provide a better estimate of the problem.22 Cultures of endoscopes performed immediately before a procedure and for routine microbiological surveillance suggest that at least 2-4% of endoscopes, including gastroscopes, colonoscopes, and duodenoscopes are transmitting bacteria.23–26 Transmission of antibiotic-sensitive enteric bacteria at gastroscopy and colonoscopy rarely causes clinical illness; however, transmitted bacteria may colonize the patient.27,28 The recent outbreaks were only identified because of the distinctive features of the CPE, the antibiotic resistance.29 CPE is acting as a marker of transmission, and the emergence of CPE has exposed long-standing flaws in endoscope reprocessing.30 Many of the problems associated with recent outbreaks are well recognized problems from the past, including breaches of cleaning and disinfection protocols, often failure to dry before storage, and occult endoscope defects that compromise cleanability. However, there are also outbreaks where cleaning and disinfection were performed according to guidelines, and the manufacturer can find no fault in the endoscope. Recent publications have found that current reprocessing standards do not provide a reasonable level of safety and effectiveness.31–34 In response to outbreaks, the FDA's May 2015 Advisory Panel35 encouraged facilities to consider supplemental measures, including double reprocessing between patients, ethylene oxide sterilization, or the use of a liquid sterilant processing system. About 15 months after these recommendations were made, a survey of providers performing ERCP in the USA found 63% of centers performed double disinfection and 12% ethylene oxide sterilization.36 However, these additional measures are expensive and time-consuming, and ethylene oxide sterilization is not readily available.33 Subsequent to this advice, a randomized trial comparing the 3 reprocessing protocols, standard high-level disinfection, double high-level disinfection, and ethylene oxide sterilization, concluded that these enhanced disinfection methods did not provide additional protection against contamination.33 Another randomized trial found double high-level disinfection was no better than standard high-level disinfection.37 It is increasingly recognized that biofilms on endoscopes compromise cleaning and disinfection.34,38,39 The conditions reported as causes of outbreaks facilitate biofilm formation and growth; these include inadequate cleaning, inadequate drying, occult endoscope defects, including channel damage and breaches of reprocessing protocols. Biofilm prevention and control are core problems in reprocessing that are addressed in these guidelines The changes proposed can be broadly summarized as follows: Cleaning—carefully follow the manufacturers' updated reprocessing instructions specific for each model of endoscope. Drying—improved drying with an alcohol flush and 10 minutes of forced air after each procedure. Endoscopes should be stored in a forced-air drying cabinet. Occult endoscope defects—routine endoscope maintenance to identify and repair defects. Routine channel replacement to reduce the prevalence of occult defects and maintain a smooth cleanable channel surface. Breaches of reprocessing protocols—Establish a multidisciplinary committee to develop and implement reprocessing protocols and to perform quality control of training, the process and outcomes. NEW RECOMMENDATIONS Recommended Changes to Reprocessing and Storage Prompt attention to cleaning, disinfection, and complete drying reduces the growth of established biofilm and prevents bacteria from forming new biofilm (Table 1). TABLE 1 - Specific Recommendations for Reprocessing and Storage Activity Recommendations Precleaning Precleaning must be carried out IMMEDIATELY after use. Cleaning Cleaning (manual or using an AFER with an FDA or National approved cleaning cycle) must be carried out PROMPTLY* within 30 minutes after precleaning. Always follow the most up to date manufacturer's specific instructions for cleaning for each model of endoscope. Disinfection After manual cleaning of the endoscope, machine or manual high-level disinfection must be undertaken promptly. Endoscopes should be thoroughly rinsed with bacteria-free water after disinfection. Alcohol flush and forced-air drying After disinfection by any means the endoscope must have prompt initial alcohol flush and forced-air drying† for 10 minutes and storage in an approved forced air storage/drying cabinet‡. Drying cabinet storage Endoscopes must remain in approved forced air-drying cabinets until next patient use. Bacteriological surveillance Perform regular bacteriological surveillance of endoscopes and AFERs at intervals appropriate to local conditions and resources. Maintenance Send endoscopes for regular yearly maintenance and consider replacing the instrument channel every 2 years or according to workload (or more frequently as recommended by the endoscope manufacturer.) Notes:Duodenoscopes are considered separately.*="Promptly" in these Guidelines means within 30 minutes.†=The endoscope may be used on another patient after the initial forced-air drying but it must be placed into the storage cabinet if not immediately used for another patient procedure.‡=See section on Drying Cabinets.AFER indicates automatic flexible endoscope reprocessor. Recommended Changes for Duodenoscopes Table 2 TABLE 2 - Specific Recommendations for Duodenoscopes Endoscopy Units Performing ERCP Should… Volume of procedures Consider whether the number of ERCP procedures performed is sufficient to continue offering this clinical service. Dedicated staff and training Have dedicated staff reprocessing duodenoscopes who are aware of and have undertaken specific training in the particular problems associated with cleaning, disinfecting, and obtaining endoscope samples for bacteriological surveillance. Bacteriological surveillance Perform MONTHLY bacteriological surveillance cultures of duodenoscopes utilizing sample collection protocols that include samples from the distal lever cavity.* Duodenoscopes with positive surveillance bacterial cultures with organisms of concern detected should be sent for service (unless there is an alternative explanation eg, staff error). Have appropriate risk notification of possible MDRO transmission in their Informed Consent information. Maintenance Regardless of culture results, send duodenoscopes for regular yearly maintenance. Have instrument channels and "O-rings" replaced at least on yearly basis (or more frequently as recommended by the endoscope manufacturer). *Note:Consideration should be given to using the recently published FDA/CDC/ASM duodenoscope sample collection and culture protocol that has been validated by duodenoscope manufacturers.40MDRO indicates multi-drug resistant organisms. Endoscope Drying It is critical that drying is performed following manual or AFER reprocessing—regardless of AFER manufacturer claims Initial Drying—All endoscopes should have a preliminary alcohol flush and forced-air channel drying for 10 minutes. Storing/Drying Cabinet—After initial drying endoscopes should be promptly transferred to an approved endoscope forced air storage/drying cabinet, and channel-purge air drying commenced. This should continue until the endoscope is used again or the safe storage period has elapsed. Storage/Drying cabinets should comply with the relevant National Standard or with the European Standard EN 16442 Controlled Environmental Storage Cabinet for Processed Thermolabile Endoscopes Note: If needed, the duodenoscope can be used for another patient procedure after the initial forced air drying or before the drying cycle in the cabinet is completed. Interventions to Control CPE Transmission in the Facility CPE is spread through the fecal-oral route; the mode of transmission is often through contaminated hands of healthcare workers or contaminated fomites. Carbapenemase-producing bacteria are commonly found in hospital wastewater and also found in sinks and faucets.41 Investigations during an outbreak of an MDRO after ERCP found the culprit MDRO in sinks and in the water used to rinse the duodenoscope before disinfection.42 Guidelines for prevention and control of CPE emphasize hand hygiene, active surveillance and contact precautions, and environmental cleaning. Endoscopy units should implement national and local infection control multi-drug resistant organism guidelines. Training that improves compliance with hand hygiene reduces transmission of infection43 (Table 3). TABLE 3 - Recommendations to Control CPE Transmission Recommendations CPE status Be aware of the CPE status of your hospital. CPE infected patients Ensure that known CPE positive patients are notified to the endoscopy unit BEFORE arriving at the unit. CPE infected patients or those at high risk, who are yet to be cultured, should be examined last on the list and managed in isolation from other patients with use of a separate toilet or a commode. Clean and decontaminate procedure room after the endoscopy procedure as per specific protocols for terminal cleaning of contaminated areas. Plumbing standards Sinks, taps and plumbing should comply with the national standards to minimize the risks of spray from drains in sinks or overflow of wastewater from blocked pipes. Infection control procedures The emergence of CPE is another compelling reason to meticulously follow standard infection control procedures including hand hygiene and the use of appropriate personal protective equipment (i.e. gloves and impervious gowns for each procedure). Endoscopy units should provide regular education, and assessment of compliance with hand hygiene and environmental cleaning and decontamination. CPE indicates carbapenem-producing Enterobacteriacae. AFER Maintenance Water quality should be appropriate for the AFER. External water filters should be replaced according to an established and the internal sub-micron filters replaced as per the manufacturer's instructions for use. GUIDELINES recommendations for reprocessing are set out in international and national Recent guidelines from and the have been updated to the and the manufacturer's These guidelines will the of other national and guidelines. In all health are according to in low and countries have increasingly focused on The of endoscopy can be from the of the services, the and the of The emergence of CPE has the risk of infections after endoscopy and the of inadequate reprocessing. The of an infection with CPE are substantial in both developed and low and The risk of transmitting CPE on the prevalence of CPE in patients for endoscopy quality of reprocessing and of repair of the endoscopes. and hospital should the local prevalence of CPE to implement appropriate risk must the principles of reprocessing and be aware of the risk to patients when there is failure of endoscope should to an previous of maintenance and and that are or have a previous workload should be reported that inadequate to 2 duodenoscopes to an outbreak and the of endoscope and Endoscope reprocessing should be managed by a multidisciplinary committee reprocessing is on by The of is best managed by a multidisciplinary committee including infection control and and most The committee should use a process to and the of a quality management for both the and the process as by the Standards and The recent of the European of Endoscopy and European of and quality for endoscope and should be developed in with relevant The committee must be up to date with recent publications and internal to ensure reprocessing with recent recommendations from and In low and there may be a of and a in trained guidelines should be to specific and quality control should start with measures as of process and compliance with guidelines should be over surveillance that is more expensive and If are a local multidisciplinary committee should the and make a based on a risk assessment by local to consider include to a with more and the for a trial of a outbreaks of MDROs after endoscopy, patients may become and no clinical only to develop infections to months with mortality reported as high as a single of CPE is transmitted from 1 endoscope on multiple occasions despite reprocessing. This is best by a biofilm on the endoscope bacteria from cleaning and disinfection and acting as a for transmission of Biofilm In the of an outbreak of a following bronchoscopy, it was considered that biofilm forming in difficult to endoscopy channels to this A examined the surfaces of endoscope channels with and the of biofilm often in surface studies have also found biofilm on endoscope and on culprit endoscopes in reports of Biofilm is a of bacteria attached to a surface and to each other by an in a biofilm have than bacteria of the into biofilms are often resistant to used at recommended reprocessing CPE are in 1 by recommended of standard a safety for these However, biofilm the of the multiple of and biofilm are difficult for the disinfectant to Standard of do not the bacteria within in biofilm that in defects on endoscope channel surfaces are also by organic debris and more difficult to with standard reprocessing based on from models using artificial soils and bacteria to be using models bacteria in biofilm or Biofilm attached to the surface of an endoscope channel as a of and given bacteria in biofilms can their and be transmitted to patients during and a of facilitate biofilm growth and of bacteria. The of biofilm growth during storage and the of complete drying after reprocessing has been in the evidence indicates of endoscopes still in channels after AFER alcohol a drying and storage in a regular the endoscope of particularly the channels during storage, must be a in endoscopes cleaning and provide a for biofilms to defects may be identified by including defects in the and and the Occult defects that do not with endoscope are more difficult to identify without the endoscope or using as a of channels in working endoscopes has found and other defects on the channel of channel surfaces biofilm in these that culprit endoscopes have found other occult defects, including and routine and maintenance reduce the use of endoscopes with defects that compromise Endoscope and national guidelines routine Duodenoscopes Duodenoscopes are difficult to clean and In to the as the of patients for ERCP, and the performed also to the risk of and infection from bacteria transmitted during the procedure. The of contamination of as by positive surveillance is similar to the of contamination of and patient and the performed are in the of outbreaks after The risks of outbreaks are best addressed by specific changes to cleaning and disinfection of duodenoscopes as well as to reprocessing protocols for all endoscopes. The manufacturers' updated cleaning protocols are an important in duodenoscope reprocessing. of a quality assurance of duodenoscope cultures found that implementation of the new cleaning protocols the of positive However, manufacturers' surveillance culture studies have found positive cultures with important Drying The reprocessing step of drying has often been or carried out and is to A survey in the of reprocessing in endoscopy units performing found that of the centers did not comply with the Guidelines and did not use forced air to dry Guidelines are with another and do not always the for Recent studies have found in up to of endoscope channels after reprocessing and drying, drying guidelines to Alfa and in a that if duodenoscopes were after reprocessing there was growth of and Drying for 10 minutes with forced air this in all duodenoscopes of an alcohol flush followed by forced air drying outbreaks of infections after ERCP in the recent studies have that alcohol flush followed by 10 minutes of forced air drying was more effective than alcohol flush followed by a time of forced air endoscopes should be stored in a drying cabinet and evidence that storage of flexible endoscopes facilitates drying, the for and protection from environmental This is supported by a of surveillance cultures of patient-ready endoscopes, including gastroscopes, colonoscopes, and found that the of drying cabinets the risk of endoscope In a a forced-air drying cabinet dried endoscopes more and growth with a standard storage The science of reprocessing is including clinical and randomized trials undertaken in response to the publications of outbreaks of CPE, is being Endoscope continue to endoscope and new reprocessing instructions. drying and cleaning are in the are updated of reprocessing guidelines in response to the of information. These and other recent guidelines a multidisciplinary committee with a of and expertise to new as it is published and and reprocessing guidelines that are appropriate to the and patient reprocessing is to patient safety in

The main scientific and organizational activities of Robert A. Pshenichnov are connected with the launching the Department of Selection and Genetics of Microorganisms at the Institute of Plant and Animal Ecology of the Ural Branch of the USSR Academy of Sciences in 1971, and his appointment as the Head of this first academic institution in Perm. He is credited with the selection of research personnel and equipment of the new department with the most modern microbiological equipment of that time. The development of this research and experimental base formed the scientific directions, later transformed into the Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the USSR Academy of Science in 1988 Many areas of research have been implemented in solving applied problems. The use of the potential of continuous cultivation of microorganisms formed the basis of the method of reclamation of mine dumps proposed by Robert Alekseevich. He also successfully developed the direction related to the improvement of methods for indicating environmental pollution. Under the leadership of R.A. Pshenichnov, a new variant of bioindicator of toxicants - Microbioluminescent Toxicity Indicator (MIT), which had higher sensitivity to toxic compounds, was developed on the basis of genetically engineered strain Escherichia coli lum+. Robert Alexeevich is the author of five monographs and six issues of mutagen catalogs, as well as numerous scientific works in the field of general and infectious microbiology, genetics, mutagenesis and ecology of microorganisms.

When modern man evolved in Africa 250,000 years ago by hunting and gathering, would he dream of megalopolis and flying machines? Would he ever understand how we managed to create and expand so much that this could cause our very decline like an Icarus that reached for the sun while the wax on his wings melts? Nowadays, as we find more and more evidence of the negative impacts we have caused to the biosphere, we are also looking for solutions to write a different future, a sustainable future. In this perspective, I present how a microbcentric approach can help achieve many of the Sustainable Development Goals (SDGs) based on the knowledge that microorganisms are the engines of life on Earth. The science of microbiology has shown that most microorganisms are not pathogens but live in close association with almost any plant or animal, with many advantages that can certainly be used in an evidence-based manner. Each of the 17 SDGs can be strengthened by considering microbes – from improving or restoring soil fertility to produce food to protecting blue carbon ecosystems, such as corals and mangroves. Recognizing that these minuscule beings hold one of the keys to a sustainable future is essential, which would be achieved by strengthening research, microbial literacy actions, and cooperation among scientists, stakeholders, decision-makers, and society. Microorganisms can help us create opportunities for the future, like a Daedalus that finally gets heard by an arrogant Icarus. Keywords: Sustainable Development Goals, bioeconomy, microbial innovations, sustainability, climate change.

У статті наводиться досвід викладання мікробіології студентам-іноземцям, які навчаються українською мовою на кафедрі мікробіології та вірусології в Буковинському державному медичному університеті. Під час навчання студенти-іноземці стикаються з низкою проблем: недостатнім володінням українською мовою, часто слабкою підготовкою за профільними та спеціальними дисциплінами, відсутністю навичок самостійної роботи, несформованим алгоритмом використання на практичних заняттях теоретичного матеріалу та відчутною різницею між формами та методами навчання в українському вузі та у вищій школі на батьківщині студента. В основі організації та проведення практичних занять з іноземцями лежить принцип індивідуального підходу до кожного студента, що забезпечується використанням на заняттях завдань різного рівня складності. Самопідготовка студентів пов’язана з наявністю в них достатньої кількості навчально-методичних посібників, можливістю отримати консультативну допомогу викладача, а також з використанням технічних засобів навчання. Для кращого засвоєння студентами лекційного матеріалу викладачі наполегливо працюють над обранням оптимальної форми подання матеріалу: у вигляді схем, графологічних структур, рисунків, навчальних слайдів, відеофільмів та мультимедійних презентацій. Засвоєнню матеріалу практичного заняття сприяє комплексний контроль знань шляхом усного опитування, оцінювання виконання практичної роботи та вміння аналізувати результати досліджень та роботи висновки. З метою стандартизації оцінки знань студентів використовуються тестові питання, складені за типом ліцензійного іспиту «Крок‑1». Постійна робота над удосконаленням професійної майстерності, використання в навчальному процесі різноманітних випробованих часом та новітніх форм і методів викладання дозволяють добре розв’язувати сучасні завдання з підготовки спеціалістів із числа іноземних громадян.

Fields and fungicides: mixing microbiology and social science

Aerosol transmission of human pathogens: From miasmata to modern viral pandemics and their preservation potential in the Anthropocene record

Move Structure of Malaysian Undergraduate Microbiological Science Thesis Abstracts

Lippincott Illustrated Reviews Microbiology.

Intra-abdominal infections(IAIs) are common surgical emergencies and complications, which usually need multidisciplinary management including surgeons, intensivists, infectious disease experts, microbiologists, and clinical pharmacists. Based on international and domestic guidelines and recent advances, a number of experts' statements of consensus, with a problem-oriented approach, were made on the cornerstones of effective treatment of IAIs such as early recognition, etiology identification, adequate source control, and appropriate antimicrobial therapy. Main recommendations include concepts of intra-abdominal infection, pathoqen diagnosis precautions; surgical intervention principles and strategies of specific causes including acute appendicitis, upper gastrointestinal perforation, lower gastrointestinal perforation, acute biliary infection, liver abscess, severe acute pancreatitis, pancreatic fistula, biliary fistula, anastomotic leakage, gastrointestinal perforation, as well as perforation due to endoscopic procedure etc.; principles of antimicrobial therapy, dosage of antibiotics in specific population and pathophysiological state; and systematic support of severe infection such as early resuscitation and nutrition support.

Introduction: As predoctoral dental students navigate through the dental curriculum, the knowledge of clinical dentistry is progressively gained from didactic lecture courses. The goal of this study was to evaluate whether there is an association between endodontic didactic knowledge and endodontic preclinical skills. Methods: The study utilized the existing preclinical endodontic assessments of 2nd-year dental students (105 students). Using ExamSoft, the nonclinical questions (e.g., microbiology and pulp biology) were excluded from the final didactic assessment, and a new didactic grade was calculated and assigned to each student. Each endodontic skill (access preparation, instrumentation and obturation, and combined) was matched with corresponding clinically related questions in the didactic assessment. For each student, the matched didactic grade was weighted against the practical grade using Kendall's τ correlation coefficient. To determine the effect of each predictor variable (didactic score and gender), an ordinal logistic regression was used. Results: For all portions of the examination, the correlation between preclinical and didactic grades was significant, P Conclusion: Only a weak association was found between didactic performance and preclinical skills. Lectures on clinical topics should be improved and sequenced accordingly to better link the theory of endodontic technique to the hands-on simulation laboratory.