Microbiological Material Research Articles

Research of Bacterial Contamination of The Surgical Field During Phacoemulsification

Conditionally pathogenic conjunctival microflora is the most frequent cause of endophthalmitis after cataract phacoemulsification (“PEC”).The purpose. To evaluate the sterility of the surgical field and anterior chamber fluid (ACF) during elective (PEC).Materials and methods. 75 eyes were examined. Patients in the group I underwent standard treatment of the surgical field, in the group IA — the standard technique was supplemented with mechanical delimitation of the upper eyelid margin, in group II additional treatment of the surgical field with povidone iodine after blepharostat installation was performed. Microbiological material was collected preoperatively from three loci (conjunctival cavity, eyelid free margin, lacrimal ducts) and at different stages of surgery from the conjunctival cavity (after standard treatment, after blepharostat placement, after additional treatment). In addition, anterior chamber fluid (ACF) was examined — also during surgery. Microorganisms were identified by the MALDI­-TOF method, sensitivity was determined by the disc­diffusion method.Results. S. epidermidis prevails among all microorganisms. The percentage of the surgical field contamination by S.epidermidis after blepharostat setting was 33,33 %, 26 % and 40 % in the I, IA, II groups respectively; after additional treatment 6,6 % in the II group; in the ACF it was found in 23,33 %, 13 % and 0 % in the I, IA, II groups respectively.Conclusion. The additional treatment of the surgical field is the most effective technique to reduce the contamination of the conjunctival cavity by S.epidermidis after placing a blepharostat and the only one in the study to ensure its absence in the ACF. Standard treatments with and without mechanical delineation are less effective and do not prevent the contamination of the ACF.

Review on the biosafety measures for the prevention and control of SARS-CoV-2 infections in healthcare settings

The novel corona virus, Severe Acute Respiratory Syndrome Corona Virus 2 (SARS‐CoV‐2) is a deadly respiratory disease. The major causes of the rise of new variants of SARS-CoV-2 are genetic mutations and recombination. The club-shaped spike (S) projections of glycoprotein on the virus's envelope has interaction with the host cell’s ACE2 (Angiotensin-Converting Enzyme 2) receptors to enable the entrance of infectious virus particles. With-Corona’ is possible only when antiviral and vaccination are available. Disease prevention and control is based on new biosafety technologies and guidelines which improve ways to safely handle microbiological material. Corona pandemic is a serious biosafety event. Thus, biosafety is being valued worldwide and applied in medical field. This review presents the current biosafety standards of the COVID-19 pandemic in a comprehensive manner from a fragmented or wide variety of biosafety guidelines and articles available.

IMPROVEMENT OF THE METHODOLOGY FOR OPTIMIZING THE PARAMETERS OF COMPLEX SYSTEMS UNDER THE INFLUENCE OF THERMAL LOAD SOURCES

The article investigates some aspects of solving the problem of constructing mathematical models for optimizing the parameters of local laser action on multilayer microbiological systems. The constraints on the desired laser parameters and on the temperature field in the material subject to laser fission are set. Verification of compliance with the restrictions on not exceeding the maximum value of the temperature field requires multiple calculations of the corresponding temperature field in the microbiological material. Control over the temperature field limits ensures the viability of its parts during the biotechnological process of laser fission. To construct adequate optimization mathematical models, the author substantiates the adequacy of the computational mathematical models describing the state of a microbiological system under the action of laser radiation sources. A multi-point boundary value problem with a system of inhomogeneous differential heat conduction equations for a multilayer microbiological medium is investigated and the correctness of this problem is substantiated for minor perturbations of the right-hand side of the differential equation. The results obtained in the article guarantee the adequacy of applied optimization mathematical models for finding rational values of technical parameters of laser emitters. In order to improve the accuracy of optimization of the technical parameters of laser emitters, the article presents a mathematical modeling of the preparatory stage of embryo defrosting. The presented mathematical models and methods of their implementation are necessary to improve the quality of embryo defrosting. Improvement of the methodology for solving applied biotechnological problems will certainly lead to the complication of mathematical models, but it will increase the accuracy of calculation and optimization of technical parameters of the biotechnological process of laser embryo division.

Prospecção de Lactobacillus plantarum e Enterococcus faecium com potencial uso probiótico espécie-específico na aquicultura ornamental de Betta splendens Regan, 1910

ABSTRACT The research evaluated the isolation, identification, and prospection of species-specific beneficial bacteria in fish farming of ornamental fish Betta splendens. For this, the microbiological material was obtained from the intestinal tract of healthy specimens, with bacterial growth in selective culture medium Man Rogosa Sharped (MRS). Sixteen strains were isolated based on the response of in vitro tests of catalase, Gram, nilin blue, hemolytic activity and antibiogram to pathogens Aeromonas hydrophila, Pseudomonas aeroginosa, Enterococcus durans and Escherichia coli. Of the isolated strains C1BS and C5BS, they showed the best responses, which were later identified by the Maldi-TOF method as Lactobacillus plantarum and Enterococcus faecium. Due to the performance of lactic acid strains in in vitro tests and the bibliographic record of their performance as probiotics, the species have great potential for species-specific use in the ornamental production of Betta splendens.

Etiological Bacterial Spectrum of Purulent Inflammation of Cervical Lymph Nodes in Children With Unknown Causes of Lymphadenopathy.

In the management of the non-specific purulent inflammation of the cervical lymph nodes in children, not only surgical treatment to evacuate the available pusbut also antibacterial therapy is mandatory. Knowledge of the spectrum of the bacterial causative agents of this disease is of fundamental importance for it. This retrospective study included 66 patients with purulent inflammation of the cervical lymph nodes with a mean age of 5.79 years, ranging from 29 days to 17 years, who were hospitalized for the period 2015-2022 in the three pediatric clinics at the St. Marina University Multispecialty Hospital for Active Treatment in Varna, Bulgaria, and operated by an oral or maxillofacial surgeon, in which for microbiological examination material from the suppurated lymph node was taken and analyzed first by direct microscopy for gram-positivity and then on a biochemical machine identifier VITEK(bioMérieux, Marcy-l'Étoile, France). In all of them, the patients and their parents did not provide anamnestic data explaining the etiology of their infectious diseases of the cervical lymph nodes. There was no clear cause that would explain the occurrence of cervical lymphadenopathy - skin infections, dental diseases, diseases of the ears and upper and lower respiratory tracts, onco-hematological diseases, and others. During the medical examination by pediatricians and surgeons, no entrances to the infection were found, but only local signs of inflammation (redness, swelling, and pain), increased levels of blood markers of inflammation (leukocytes, neutrophils, erythrocyte sedimentation rate, and C-reactive protein) and there was imaging evidence (ultrasound, magnetic resonance imaging, and computed tomography) of a purulent collection in the lymph nodes and/or in the soft tissues surrounding them. Exudate without microbial growth was found in 22 of the cases. The main causative agents of purulent lymphadenopathy are gram-positive bacteria (n=32, 72,73%) - Staphylococcus aureus (n=16), Staphylococcus haemolyticus (n=8), Beta-hemolytic streptococcus(n=2), and gram-positive mixed resident microflora (n=6). However, in 27.27% (n=12) of all 44 patients described in this article with isolated pathogens, cultures were gram-negative. These are Bartonella henselae (n=4), Klebsiella pneumoniae (n=4), Klebsiella oxytoca (n=2), and Flavimonas oryzihabitans (n=2).

The International Society for Biological and Environmental Repositories Presents Abstracts from Its Annual Meeting

The International Society for Biological and Environmental Repositories Presents Abstracts from Its Annual Meeting

Progress in the Detection of Cerebrospinal Fluid by Raman Spectroscopy

Abstract: As a precious sterile body fluid, cerebrospinal fluid (CSF) examination plays an important role in the diagnosis of many clinical diseases. Early diagnosis can significantly improve these diseases survival rate. Raman spectroscopy is a scattering spectrum that has been used for the research and analysis of molecular structures. It has been widely used in many fields, such as protein detection, tumor genes, microbiological pathogen compound materials, and food and medical monitoring, with high sensitivity and specificity. In this review, we briefly introduce the mechanism of Raman spectroscopy and summarize its progress in detecting cerebrospinal fluid, mainly focusing on the application of neurodegenerative diseases by Raman spectroscopy. Meanwhile, we also prospect the development of Raman spectroscopy in the detection of CSF and other fluids.

Enhancing cell resistance for production of mixed microbiological reference materials with Salmonella and coliforms by freeze-drying.

The reference material (RM) is a technical requirement for the quality assurance of analytical results and proficiency tests or interlaboratory comparisons. Microbiological RMs are most available in the dehydrated form, mainly by freeze-drying, and maintaining bacterial survival after preparation is a challenge. Thus, obtaining the most resistant cells is essential. Considering that bacteria present cross-response to dehydration after being submitted to an array of stress conditions, this study aimed to evaluate the influence of growth conditions on enterobacteria for the production of mixed microbiological RMs by freeze-drying in skim milk powder. Salmonella enterica serovar Enteritidis, Cronobacter sakazakii, Escherichia coli, and Citrobacter freundii were grown in a minimal medium with 0.5M NaCl and 0 to 5.0mM of manganese sulfate (MnSO4) until stationary phase. Salmonella Enteritidis presented an increased resistance to dehydration in the presence of Mn, while C. sakazakii was the most resistant to freeze-drying and further storage for 90days. Mixed microbiological RMs were produced by freeze-drying and containing Salmonella Enteritidis and coliforms in skim milk powder with 100mM of trehalose and the Salmonella survival rate was 91.2 to 93.6%. The mixed RM was stable after 30days at -20°C, and Salmonella and coliforms were detected by different methods being, the Rambach Agar the best for the bacterial differentiation. The results showed that the culture conditions applied in this study resulted in bacterial cells being more resistant to dehydration, freeze-drying, and stabilization for the production of mixed microbiological RMs more stable and homogeneous.

Universal Preparation Strategy for Ultradurable Antibacterial Fabrics through Coating an Adhesive Nanosilver Glue.

Microbiological protection textile materials played an important role in the battle against the epidemic. However, the traditional active antimicrobial treatment of textiles suffers from narrow textile applicability, low chemical stability, and poor washability. Here, a high-strength adhesive nanosilver glue was synthesized by introducing nontoxic water-soluble polyurethane glue as a protectant. The as-prepared nanosilver glue could adhere firmly to the fiber surfaces by forming a flexible polymer film and could encapsulate nanosilver inside the glue. The as-prepared nanosilver had a torispherical structure with diameter of ~22 nm, zeta potential of −42.7 mV, and good dispersibility in water, and it could be stored for one year. Further studies indicated that the nanosilver glue had wide applicability to the main fabric species, such as cotton and polyester fabric, surgical mask, latex paint, and wood paint. The antimicrobial cotton and polyester fabrics were prepared by a simple impregnation–padding–baking process. The corresponding antimicrobial activity was positively correlated with nanosilver content. The treated fabrics (500 mg/kg) exhibited ultrahigh washing resistance (maintained over 99% antibacterial rates for 100 times of standard washing) and wear resistance (99% antibacterial rates for 8000 times of standard wearing), equivalent breathability to untreated fabric, improved mechanical properties, and good flexibility, demonstrating a potential in cleanable and reusable microbiological protection textiles.

Draft Genome Sequences of Eight Bacilli Isolated from an Ancient Roman Amphora.

ABSTRACTPaleomicrobiology, the study of ancient microbiological material, allows us to understand different evolutionary phenomena in bacteria. In this study, eight bacilli isolated from an ancient Roman amphora, which dates to the IV to V sec. AD, were sequenced and functionally annotated.

ANALYSIS OF COGNITIVE ASSESSMENT INSTRUMENTS IN GENERAL BIOLOGY LEARNING MEDIA

The existence of cognitive assessments in learning media that does not require much consideration for their feasibility as a measuring tool is one of the problems that arise because it can cause misinformation obtained by the teacher. The purpose of this study was to determine the construct validity and content of the cognitive assessment instrument and the validity of the questions, the reliability of the questions, the distinguishing power of the questions, and the level of difficulty of the questions on the cognitive assessment instruments to be used in general biology learning media. The research method used is descriptive quantitative through a survey involving one expert and 58 students of the Biology Education study program of IKIP Budi Utomo. The research limitation is a cognitive assessment of microbiology material in general biology courses. The type of instrument analyzed is a test. The results obtained are that the cognitive assessment instrument has a valid level of validity from the aspect of construct and content, the validity of the questions in the valid category, the reliability of the questions is moderate, the discriminatory power of the questions is good, and the level of difficulty of the questions is moderate. The conclusion is that the cognitive assessment instrument is appropriate to be used as an appropriate measuring tool for microbiology material in general biology learning media.

LOCAL MICROBIOLOGICAL MONITORING IN THE DEPARTMENTS OF ANESTHESIOLOGY AND INTENSIVE CARE OF THE GRODNO UNIVERSITY CLINIC

The aim of the study was to conduct a comparative and retrospective analysis of the spectrum and sensitivity to antimicrobial drugs of clinically significant microorganisms in two departments of anesthesiology and intensive care of the Grodno University Clinic. Material and methods. A retrospective analysis of the data for 5 years of microbiological material obtained from patients with diseases caused by pathogenic and opportunistic bacteria from two departments of anesthesiology and resuscitation was carried out. Results. According to the results of five-year dynamics, the seeding rate of some microorganisms (K. pneumoniae, A.baumannii, coagulase-negative staphylococci) increased in intensive care units by 2-2.5 times. At the same time, there is a decrease in the sensitivity of these microorganisms (up to 15-30%) to antimicrobial drugs, including the reserve group. Conclusions. Constant microbiological monitoring makes it possible to objectively determine the resistance of microorganisms to antimicrobial drugs, implement the optimal tactics for the use of antimicrobial drugs and prevent an increase in the number of resistant strains.

PENYUSUNAN MEDIA POSTER DALAM PEMBELAJARAN BIOLOGI: MIKROORGANISME LOKAL (MOL) PADA TANAMAN JAGUNG SEBAGAI BIOAKTIVATOR PAKAN TERNAK

Posters are one of the media by presenting plots and pictures in explaining a material to make it easier for students to understand. The purpose of this study was to make a media poster based on research results by local microorganisms (MOL) in corn plant waste as animal feed bioactivators. This study used MOL from cattle rumen and molasses as a control and corn plant waste (leaves, stems and cobs) as a treatment. The method was experimentally by visually observing the morphological characteristics of the microbes, the gram staining of the microbes and the pH value of the MOL solution. The results of morphological observations found 12 types of bacterial isolates on NA media and there were nine isolates of gram-positive and three gram-negative bacteria. The most optimal pH value is at the 1% level with a value of 6.8. The addition of corn plant waste at 1% increases the type of bacteria and optimizes the pH value so that it has the potential to increase the quality of local microorganisms (MOL) for animal feed. Poster media can be used effectively by teachers and students as a medium for learning biology microbiology material for class X High School.
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17. Comparative Assessment of a Machine Learning Model and Rectal Swab Surveillance to Predict Hospital Onset Clostridioides difficile

BackgroundHospital onset Clostridioides difficile infection (HO-CDI) is associated with significant morbidity and mortality. Screening individuals at risk could help limit transmission, however swab-based surveillance for HO-CDI is resource intensive. Applied to electronic health records (EHR) data, machine learning (ML) models present an efficient approach to assess patient risk. We compare the effectiveness of swab surveillance against daily risk estimates produced by a ML model in detecting patients who will develop HO-CDI.MethodsPatients presenting to Michigan Medicine’s ICUs and oncology wards between June 6th and October 8th 2020 had rectal swabs collected on admission, weekly, and at discharge from the unit, as part of VRE surveillance. We performed anaerobic culture on the residual media followed by a custom, multiplex PCR on isolates to identify toxigenic C. difficile. Risk of HO-CDI was calculated daily for each patient using a previously validated EHR-based ML model. Swab results and model risk scores were aggregated for each admission and assessed as predictors of HO-CDI. Holding sensitivity equal, we evaluated both approaches in terms of accuracy, specificity, and positive predictive value (PPV).ResultsOf 2,044 admissions representing 1,859 patients, 39 (1.9%) developed HO-CDI. 23.1% (95% CI: 11.1–37.8%) of HO-CDI cases had at least one positive swab. At this sensitivity, model performance was significantly better than random but worse compared to swab surveillance—accuracy: 87.5% (86.0–88.9%) vs. 94.3% (93.3–95.3%), specificity: 88.7% (87.3–90.0%) vs. 95.7% (94.8–96.6%), PPV: 3.8% (1.6–6.4%) vs. 9.4% (4.3–16.1%). Combining swab AND model yielded lower sensitivity 2.6% (0.0–8.9%) compared to combining swab OR model at 43.6% (27.3–60.0%), and yielded PPV 7.1% (0.0–25.0%) vs. 43.6% (27.3–60.0%) respectively (Figure 1).Figure 1. Surveillance & risk score performance. Binary classification performance metrics of ML model (Model), toxigenic C. difficile rectal swab surveillance (Swab), and combination approaches (Model AND Swab and Model OR Swab), reported in terms of percentage points. Bold numbers highlight the best performing approach for a given performance metric. The combined approach of monitoring the Model AND Swab yielded the highest accuracy 97.5% (95% confidence interval: 96.8%, 98.1%), it also had the highest specificity 99.4% (99.0%, 99.7%). The combined approach of monitoring the Model OR Swab yielded the highest sensitivity 43.6% (27.3%, 60.0%) and negative predictive value (NPV) 98.7% (98.2, 99.2%). Using the Swab alone yielded the highest PPV 9.4% (4.3%, 16.1%) and F1 score 13.3% (6.2%, 21.8%). These results highlight the complementarity of the model and swab-based approaches.ConclusionCompared to swab surveillance using a ML model for predicting HO-CDI results in more false positives. The ML model provides daily risk scores and can be deployed using different thresholds. Thus, it can inform varied prevention strategies for different risk categories, without the need for resource intensive swabbing. Additionally, the approaches may be complimentary as the patients with HO-CDI identified by each approach differ.Disclosures Vincent B. Young, MD, PhD, American Society for Microbiology (Other Financial or Material Support, Senior Editor for mSphere)Vedanta Biosciences (Consultant) Krishna Rao, MD, MS, Bio-K+ International, Inc. (Consultant)Merck & Co., Inc. (Grant/Research Support)Roche Molecular Systems, Inc. (Consultant)Seres Therapeutics (Consultant)

Theoretical and methodological approach to the study of technological processes

The article investigates some issues of complex systems analysis and synthesis that contain local, discrete sources of temperature fields. The emphasis of the author's research lies in the calculation and optimization of the parameters of the laser action on the embryo. The biotechnological process is described by the boundary value problem of a non-stationary, multidimensional differential equation of thermal conductivity which satisfies the boundary conditions of heat flux, beginning and end of the laser action process. The author proposes an algorithm for calculation and optimization of the control parameters of laser action on multilayer microbiological material. The object of the study is the embryo under the action of a laser beam for fission. As a first approximation, at the stage of calculation and optimization of the parameters of the biotechnological process, the embryo is considered as a homogeneous body. The values of thermophysical characteristics are calculated by the method of expert evaluation of the parameters of the emitters. The correctness of the boundary value problem of the process of laser action on the embryo is proved by the author using the method of pseudodifferential operators. Seeking the solution of the boundary value problem in the form of the series, the analytical function of the temperature field distribution is obtained using the Fourier method of the separated variables in the article. Using the method of indeterminate coefficients, the author found the temperature of the laser action on the embryo. Using the approximate gradient method of finding local extrema and the method of directed search of local extrema, it is possible to obtain rational values of optimized parameters of the biotechnological process. The author outlines possible approaches for optimization of technical parameters of laser emitters. In his opinion, the results of research can be considered fundamental for the calculation and optimization of the parameters of the laser action on the embryo, taking into account the multilayer structure of the latter.

Fourier Transform Infrared (FTIR) Spectroscopic Analyses of Microbiological Samples and Biogenic Selenium Nanoparticles of Microbial Origin: Sample Preparation Effects.

To demonstrate the importance of sample preparation used in Fourier transform infrared (FTIR) spectroscopy of microbiological materials, bacterial biomass samples with and without grinding and after different drying periods (1.5–23 h at 45 °C), as well as biogenic selenium nanoparticles (SeNPs; without washing and after one to three washing steps) were comparatively studied by transmission FTIR spectroscopy. For preparing bacterial biomass samples, Azospirillum brasilense Sp7 and A. baldaniorum Sp245 (earlier known as A. brasilense Sp245) were used. The SeNPs were obtained using A. brasilense Sp7 incubated with selenite. Grinding of the biomass samples was shown to result in slight downshifting of the bands related to cellular poly-3-hydroxybutyrate (PHB) present in the samples in small amounts (under ~10%), reflecting its partial crystallisation. Drying for 23 h was shown to give more reproducible FTIR spectra of bacterial samples. SeNPs were shown to contain capping layers of proteins, polysaccharides and lipids. The as-prepared SeNPs contained significant amounts of carboxylated components in their bioorganic capping, which appeared to be weakly bound and were largely removed after washing. Spectroscopic characteristics and changes induced by various sample preparation steps are discussed with regard to optimising sample treatment procedures for FTIR spectroscopic analyses of microbiological specimens.

Fabrication of N-halamine polyurethane films with excellent antibacterial properties

Abstract An N-halamine precursor, namely, 2-amino-5-(2-hydroxyethyl)-6-methylpyrimidin-4-one (AHM), was used as a chain extender in the preparation of a series of N-halamine polyurethane (PU) films, in order to also instill antibacterial properties. The mechanical properties, thermodynamic performance, and antimicrobial performance of the functionalized PU films were systematically studied. The results showed that the addition of AHM could improve the thermodynamic and mechanical properties of the developed PU films. Conducting tests in the presence of Escherichia coli and Staphylococcus aureus as the model microorganisms revealed that prior to chlorination the antibacterial properties of the chlorinated PU-AHM-Cl films improved significantly relative to the analogous films. The excellent antibacterial properties and the overall superior performance of the PU-AHM-Cl films allow their potential application in microbiological protection materials and related fields.

A Design of Centrifugal Pumps With 250 Liters / Second Capacity for Water Supply at Boarding School in Cibubur, Jakarta Timur

This research was motivated by the existence of environmental pollution problems caused by manufacturing activities that use styrene plastic materials as part of the packaging for electronic products. To solve these problems, innovative uses of environmentally-friendly materials from natural resources are necessary. The substitution of natural materials for the synthetic materials used in manufacturing is vitally important in the effort to eliminate the negative effects of environmental pollution. The use of trial-and-error methods to create and test replacement materials derived from renewable natural resources will allow sustainable manufacturing development. In this study, we assessed the feasibility of replacing styrene plastic materials, which commonly are used in the packaging of manufactured goods, with waste materials from banana stems so that the packaging of manufactured goods can be an environmentally-friendly product. The resulting banana stem of new and renewable material substitution for sustainable manufacturing, namely the M4 + T + P3 + A + t6 model. Micro-biological banana stem material is broken down easily in the soil, so this material does not disturb the environment, while styrene materials do not break down in the soil. The implication of this research is the packaging of manufactured products can be sustainable.

Design and Build Models of Banana Stem as Material Substitution for Sustainable Manufacturing

This research was motivated by the existence of environmental pollution problems caused by manufacturing activities that use styrene plastic materials as part of the packaging for electronic products. To solve these problems, innovative uses of environmentally-friendly materials from natural resources are necessary. The substitution of natural materials for the synthetic materials used in manufacturing is vitally important in the effort to eliminate the negative effects of environmental pollution. The use of trial-and-error methods to create and test replacement materials derived from renewable natural resources will allow sustainable manufacturing development. In this study, we assessed the feasibility of replacing styrene plastic materials, which commonly are used in the packaging of manufactured goods, with waste materials from banana stems so that the packaging of manufactured goods can be an environmentally-friendly product. The resulting banana stem of new and renewable material substitution for sustainable manufacturing, namely the M4 + T + P3 + A + t6 model. Micro-biological banana stem material is broken down easily in the soil, so this material does not disturb the environment, while styrene materials do not break down in the soil. The implication of this research is the packaging of manufactured products can be sustainable.

Pengembangan Handout Mikrobiologi Berbasis Hasil Penelitian Antagonisme Fungi Antagonis terhadap Fungi Patogen pada Tanaman Stroberi

The objectives of this study were: 1) to produce a Microbiology handout based on the results of the study of Antagonistic Fungi Antagonism against Pathogenic Fungi in Strawberry Plants; and 2) testing the feasibility and practicality of the handout. This type of research is development research. The development model used in this research is the ADDIE model which consists of 5 stages, namely: Analyze, Design, Develop, Implement, and Evaluate. The handout material was selected based on the results of the needs analysis by the respondent students of the Biology Study Program, FMIPA, State University of Malang. Handout eligibility data obtained from the results of validation by microbiology material experts and learning media experts. Meanwhile, the handout practicality data was obtained from the readability assessment results of Biology Study Program students who were currently taking the Microbiology course. The results showed that, the percentage of eligibility of handout material by the expert validator of microbiology material was 99.53% with very high or very valid eligibility criteria. The percentage of eligibility of handouts by instructional media expert validators is 98.14% with very high or very valid eligibility criteria. The percentage of practicality tests by students who were taking the Microbiology course resulted in 89.89% with very high practicality criteria. The results showed that research results-based Microbiology handouts were very feasible and practical to be used in microbiology learning activities.