Microbiological Indicators Research Articles

Hydrochemical And Bacterial Properties Of Water Bodies Of The East European Plain During Low Water Period

This paper is devoted to the study of the chemical and biological properties of river waters and the relationship between them. We examined the hydrochemical and bacterial properties of surface water in 3 waterbodies: the Mezha River, a pond in Zapovedny village (Central Forest Nature Reserve, Tver Oblast) and the lower reaches of the Don River (Rostov Oblast). The biodiversity of bacteria was determined based on their growth on dissolved organic matter (DOM). Among bacterioplankton capable of growing on DOM as the only source of carbon, the predominant species in the Don River were Pseudomonas and Deinococcus, in the Mezha River – Pseudomonas and Janthinobacterium, in the pond – Arcicella. In terms of sanitary and microbiological indicators, none of the waterbodies complied with the Sanitary Rules and Regulations 1.2.3685- 21 for surface waters. The content of most of the studied elements and heterotrophic bacteria in stagnant waterbodies was lower than in flowing streams. The concentration and activity of heterotrophic bacteria in the studied waters correlated positively with the content of biophilic elements in them and negatively with the absence of a current. We showed that there is a strong correlation between bacterial and chemical indicators due to common factors: eutrophication, features of the physical and geographical conditions of the territory, and the presence of a current or animal waste products.

Microbiological quality and cultivable bacterial community of fresh and ripened Minas cheeses made from raw and pasteurised milk

The microbiological and physicochemical characteristics of 56 samples of Brazilian fresh (Minas frescal cheese, MFC) and ripened (Minas padrão cheese, MPC; Minas artisanal cheese, MAC) cheeses produced from pasteurised (MFC and MPC) or raw milk (MAC) were evaluated. Significant differences were observed between samples, with a positive emphasis on microbiological quality for MPC. MALDI-TOF MS identified a diverse bacterial community from the 808 colonies isolated from different culture media. Staphylococcus aureus (34.32%) and Escherichia coli (35.59%) were the most isolated species. The eae gene, which confers virulence on E. coli strains, was observed in 23 samples (41.07%), and in 21.05%, 27.78%, and 73.68% of MFC, MPC and MAC samples, respectively. There was no correlation between the evaluated indicators and the presence of eae-positive cheese samples. The choice of the ideal microbiological indicator must be specific, considering the physicochemical characteristics and the raw milk of the different types of cheese.

SOYBEAN BASE FERTILIZATION: CHEMICAL AND MICROBIOLOGICAL SOIL INDICATORS AND CROP YIELD

Recent research suggests that soybean yield could be doubled in Brazilian conditions, implying that the base fertilization is pivotal, as it might influence chemical and microbiological soil indicators and, hence, the crop grain yield. The current study had the goal of assessing alterations in the chemical and microbiological soil indicators, in the short term, as well as the soybean biometric and grain yield performance as a function of different fertilizers for base fertilization, in two sowing periods. An experiment comprised of two sowing periods was carried out in the 2019/2020 season on a dystrophic Red Latosol, in north Parana state. Five treatments were assessed, comprising: 1) control; 2) mineral fertilizer; 3) organomineral fertilizer; 4) mineral fertilizer mixed with granulated gypsum; and 5) slow release mineral fertilizer. A randomized block design with four replicates was adopted. The following variables were assessed: chemical and microbiological soil indicators; final stand; first pod insertion height; plant height; stem diameter; number of pods per plant; number of grains per plant; number of grains per pod; grain mass per plant; and one thousand grain mass and grain yield. There is no base fertilization effect on the chemical soil indicators in the short term, however, there is an effect on the microbiological soil indicators. Soybean biometric and grain yield performance is decreased with the delayed sowing period, regardless of the type of fertilizer utilized for base fertilization. Analyzing a set of soil quality indicators enables precise and judicious results to be gathered on management practices in the soil environment.

Determining the qualitative parameters of powder from the stalks of garden purslane (Portulaca oleracea L.) and its application in the production of functional bakery products

Bread and bakery products are the most common food products that have a significant impact on the biological and energy value of the human diet. The presence of proteins, fats, vitamins, mineral components, and dietary fiber in them depends on the assortment, recipe, and cooking technology. The use of dried purslane in powder form as a semi-finished product (raw material and additive) and the development of various technologies with it at the same time can provide the population with a wide range of food products in the diet. The aim of this work is to determine the quality indicators of the powder from the stems of garden purslane (Portulaca oleracea L.) and its application in the production of functional bakery products. This will make it possible to use them as a protein-carbohydrate-mineral and multicomponent fortifier to obtain functional bakery products. This paper reports the results of studying in a laboratory the powder from the stalks of garden purslane (PP), growing under the conditions of the Absheron district of Baku, Azerbaijan. Its general chemical and mineral composition, some physical-mechanical and microbiological indicators and safety indicators of PP have been studied, the doses of its introduction into yeast dough have been substantiated. By organoleptic and physical-chemical methods, the technological indicators of the quality of yeast dough and finished products with PP powder for the production of functional bakery products were determined. Powder from garden purslane stems was used in the production of functional bakery products, and a recipe and technological scheme for the production of "Health" buns using purslane powder were devised

ASSESSMENT OF HIGH-PROTEIN QUALITY FEED ADDITIVES FROM BY-PRODUCTS MANUFACTURE OF SUNFLOWER OIL

The article states that until 2022, Ukraine will occupy the first place in the world food market in the production of sunflower, sunflower oil and export of sunflower oil. The production of sunflower has always been sufficiently profitable, and the products of its processing competitive on the domestic and world markets. They are also an important component of food and feed protein resources. The production of complete ration compound feed for farm animals and poultry is restrained by a shortage of protein components, a low level of sanitary and hygienic safety of protein raw materials of animal origin. This leads to a decrease in the efficiency of feed, their costs and, as it should be, a shortage of livestock products. Cake and meal are high-protein raw materials, which are obtained in the amount of 1/3 of the mass of processed seeds during the production of sunflower oil. The fodder value of sunflower cake and meal is given. Factors that negatively affect the nutritional value and availability of nutrients of sunflower meal in the feed of farm animals and poultry were analyzed. The choice of components for the production of high-protein feed additive is theoretically justified. The indicators of the quality of sunflower seeds and by-products of their processing, which meet the requirements of DSTU, have been determined. On the basis of theoretical and experimental data, a structural scheme for the production of a high-protein feed supplement based on sunflower cake and meal is proposed, which involves the preliminary preparation of the by-products of sunflower oil production - cake and meal, with the following dosage in the amount: 75% sunflower meal, 10% sunflower cake and 15% limestone flour. The physical properties and microbiological indicators of the quality of a highprotein feed additive were experimentally determined, and it was determined that, compared to a loose, granular high-protein feed additive, it has the following advantages: increased nutritional value, has a greater volumetric weight, sanitary quality, which leads to saving space during transportation and better storage.

Legal regime of activities carried out on the basis of technical regulations of the Eurasian Economic Union and the Customs Union

The article notes that currently, the Technical Regulations of the Eurasian Economic Union are in force and the previously adopted technical regulations of the Customs Union continue to operate. Technical regulations in order to ensure product safety, establish requirements for the manufacturer at all stages of production. The Technical Regulations of the EAEU and the CU are legal acts of interstate regulation, are acts of direct action, they are mandatory for application on the territory of all countries that are members of the EAEU.Product safety requirements are checked by certification bodies when issuing a certificate of conformity. At the same time, technical regulations give them the right to exercise control in the production process. The state authorities control the products after they are put into circulation, but not the production process itself. Taking into account the considered features of control, the article concludes that the legal regime of the activities of economic entities engaged in entrepreneurial activity on the basis of technical regulations refers to the legal regime of special control.Detection of unsafe products during inspection means a significant violation of product quality requirements. The article states that the seller should not be responsible for the identified deviations in products according to microbiological indicators. This violation is committed at the stage of the technological process of manufacturing the product, for which the manufacturer must be responsible. It is concluded that Article 14.43 of the Administrative Code of the Russian Federation needs to clarify the subject of responsibilit.

Microbiome of High-Rank Coal Reservoirs in the High-Production Areas of the Southern Qinshui Basin.

To study the distribution features of microorganisms in distinct hydrological areas of the southern Qinshui Basin, C-N-S microorganisms were studied using 16S RNA sequencing, metagenome sequencing and geochemical technologies, showing the high sensitivity of microorganisms to the hydrodynamic dynamics of coal. The hydrodynamic intensity of the #3 coal gradually decreased from the runoff areas to the stagnant areas. The stagnant zones have higher reservoir pressure, methane content, δ13CDIC and TDS and lower SO42-, Fe3+ and NO3- concentrations than the runoff areas. C-N-S-cycling microorganisms, including those engaged in methanogenesis, nitrate respiration, fermentation, nitrate reduction, dark oxidation of sulfur compounds, sulfate respiration, iron respiration, chlorate reduction, aromatic compound degradation, denitrification, ammonification and nitrogen fixation, were more abundant in the stagnant areas. The relative abundance of C-N-S functional genes, including genes related to C metabolism (e.g., mcr, mer, mtr, fwd and mtd), N metabolism (e.g., nifDKH, nirK, narGHI, nosZ, amoB, norC and napAB) and sulfur metabolism (e.g., dsrAB and PAPSS), increased in the stagnant zones, indicating that there was active microbiological C-N-S cycling in the stagnant areas. The degradation and fermentation of terrestrial plant organic carbon and coal seam organic matter could provide substrates for methanogens, while nitrogen fixation and nitrification can provide nitrogen for methanogens, which are all favorable factors for stronger methanogenesis in stagnant areas. The coal in the study area is currently in the secondary biogenic gas generation stage because of the rising of the strata, which recharges atmospheric precipitation. The random forest model shows that the abundance of C-N-S microorganisms and genes could be used to distinguish different hydrological zones in coal reservoirs. Since stagnant zones are usually high-gas-bearing zones and high-production areas of CBM exploration, these microbiological indicators can be used as effective parameters to identify high-production-potential zones. In addition, nitrate respiration and sulfate respiration microorganisms consumed NO3- and SO42-, causing a decrease in the content of these two ions in the stagnant areas.

Development and evaluation of consumer properties of functional canned meat

The article presents the results of a study of the combination of plant extracts of caulifl ower and stinging nettle in canned poultry and beef. In the course of the study, the optimal amounts of additives were established, as well as the organoleptic, physico-chemical, and microbiological indicators of the quality of fi nished products were examined. The article presents the main functional properties of canned meat, developed on the basis of a combination of vegetable and animal raw materials. As a result of the research, a method to increase the nutritional value and microbiological safety of canned meat is proposed.

COVID-19 Lockdown Pandemic Period Effects in Highly Impacted Aquatic Ecosystems.

Human activities severely affect aquatic ecosystems: the lockdown restrictions due to SARS-CoV-2 (COVID-19) have proved that reducing anthropogenic activities can positively influence the environment and mitigate atmospheric pollution. In addition, studies on aquatic ecosystems have highlighted the decrease in suspended matter and pollutant concentrations associated with reduced marine transport and industrial activities. We evaluated the effects of the reduction of anthropogenic activities in highly impacted water ecosystems. Three of the sampling sites were on the Tiber River's lower stretch close to the urban area of Rome (Italy), and the other two were transitional waters located in the southern Latium region coastal area. These sampling sites have been studied by our research group for at least 20 years. The present study involved analysis of chemical and physicochemical parameters, biological communities, and land use. Microbiological fecal indicators and Salmonella spp. were also analyzed. There were two sampling campaigns, the first one in May 2020 (ID II) at the end of lockdown measures, and the second one a year later, in June 2021 (ID III). The data were compared with our old data, collected from 2002 to 2015 (ID I), to evaluate the potential effects of the restrictive measures. Biotic communities were dominated by pollution-tolerant taxa, indicating eutrophication and organic pollution. Furthermore, the high concentrations of microbiological indicators was mainly due to sewage from the urban area. The results of the research indicated no significant changes from past years (ID I) to 2020 (ID II) and 2021 (ID III). In conclusion, the restrictions adopted in Italy during the pandemic period were not sufficient to mitigate the heavy pressure of urbanization and agriculture that have long affected the studied aquatic ecosystems. Environ Toxicol Chem 2023;42:966-977. © 2022 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

Microbiological Changes during Long-Storage of Beef Meat under Different Temperature and Vacuum-Packaging Conditions

We evaluated a combination of two temperatures and two packaging materials for long-term storage of vacuum-packaged (VP) beef striploins. Microbial populations and microbiome composition were monitored during refrigerated storage (120 days between 0-1.5 °C) and refrigerated-then-frozen storage (28 days between 0-1.5 °C then 92 days at -20 °C) under low-O2 permeability VP and high-O2 permeability VP with an antimicrobial (VPAM). Pseudomonas (PSE) and Enterobacteriaceae (EB) counts in VPAM samples were significantly higher (p < 0.05) than in VP samples at 28, 45, 90, and 120 days of storage. Microbiome data showed that bacteria of the genera Serratia and Brochothrix were more abundant in VPAM samples at 120 days, while lactic acid bacteria (LAB) dominated in VP samples. Frozen temperatures inhibited microbial growth and maintained a relatively stable microbiome. Refrigerated and frozen VPAM samples showed the greatest difference in the predicted metabolic functions at the end of storage driven by the microbiome composition, dominated by PSE and LAB, respectively. Although no signs of visible meat deterioration were observed in any sample, this study suggests that VP meat refrigerated and then frozen achieved better microbiological indicators at the end of the storage period.

Thermosonication Applied to Kiwi Peel: Impact on Nutritional and Microbiological Indicators.

The peels of many fruits are rich sources of nutrients, although they are not commonly consumed. If they are properly decontaminated, they can be used as healthy food ingredients reducing food waste. The objective was to apply thermosonication processes to kiwi peel and evaluate the impact on Listeria innocua survival (a non-pathogenic surrogate of L. monocytogenes) and key nutrients and quality indicators: proteins, fibers, minerals (Ca, K, Mg, Na, and P), chlorophylls, and phenolic contents. Kiwi peels were artificially inoculated with L. innocua and thermal and thermosonication treatments were performed at 55 °C and 60 °C for 30 and 15 min maximum, respectively. Bacteria were enumerated through treatment time, and quality indicators were assessed before and at the end of treatments. A Weibull model with a decimal reduction time (D-value) was successfully used in L. innocua survival data fits. Results showed that coupling temperature to ultrasound had a synergistic effect on bacteria inactivation with significant decreases in D-values. Thermosonication at 60 °C was the most effective in terms of protein, fiber, chlorophylls, and phenolics retention. Minerals were not significantly affected by all treatments. Applying thermosonication to kiwi peel was more effective for decontamination than thermal treatments at the same temperature while allowing the retention of healthy compounds.

Ganhos econômicos usando fonte orgânica de P e inoculação com bactérias solubilizadoras de P em cana-de-açúcar

ABSTRACT Biological agribusiness has grown substantially worldwide and requires efficient strategies to maintain or increase crop production. However, little is known about the real economic gains associated with belowground mechanisms in agriculture, including those of traditional crops such as sugarcane. This study aimed to identify potential microbiological indicators related to yield increase and value the soil microbiological services through the development of a structural equation model (SEM). The SEM was constructed based on a dataset from a previous sugarcane field experiment in which the effects of inoculation with phosphate-solubilizing bacteria (PSB) and the input of organomineral fertilizer were evaluated. The SEM indicated that the β-glucosidase and alkaline phosphatase activities were potential indicators of yield increases in four scenarios (current, plausible, optimistic, and futuristic). Increases of 158 and 195 t ha-1 were projected based on the β-glucosidase activity for the current and plausible scenarios, respectively. These increases resulted in economic gains of R$ 453.02 ha-1 (US$ 86.07 ha-1) for the current scenario, and R$ 1,571.53 ha-1 (US$ 298.59 ha-1) for the plausible scenario, considering the exchange rate from February 2022 (R$ 0.19 US$-1). Regardless of the scenario, bacterial inoculation was associated with increased β-glucosidase or alkaline phosphatase activity and higher yields, which translates into economic gains for sugarcane farmers.

Assessment of Water Quality and Heavy Metal Environmental Risk on the Peri-Urban Karst Tropical Lake La Sabana, Yucatán Peninsula

In the Yucatán Peninsula, anthropogenic activities such as urbanization and the final disposal of solids and wastewater critically impact aquatic systems. Here, we evaluated the anthropogenic-related environmental alteration of Lake La Sabana, located in the northern limits of one of the main cities of the Mexican Caribbean. We evaluated lake water quality, using physical, chemical, and microbiological indicators, and heavy metals in surficial sediment and fish tissue to evaluate the potential environmental risk. Multivariate analyses revealed that environmental conditions in La Sabana are spatially and temporally heterogeneous. Medium to bad water quality was determined within different basins by the National Sanitation Foundation water quality index, related to the degree of anthropogenic influence at each zone. The central-south zones displayed critical microbiological values largely exceeding national standards. Heavy metals in sediment and fish tissue such as Zn and Hg were relatively low, but Hg concentrations threaten the ecological environment. Incipient wastewater treatment and its final disposal in La Sabana are mainly responsible for the changes in the trophic status and availability of nutrients, which in turn may have promoted changes in the biological structure and aquatic plant invasions. Lake La Sabana can be considered a model of the potential and sequential effects of anthropogenic alterations in the oligotrophic karst tropical aquatic systems in the Yucatan Peninsula.

Assessment of organoleptic, microbiological and physico-chemical indicators of feed quality

Microbiological, organoleptic and physico-chemical indicators of the quality of feed selected in the Kaluga region are presented. The conditions of preparation of the studied samples are optimized. To assess the required indicators, GOST methods and traditional methods based on the use of chemical and biological methods, as well as organoleptic analysis, were used.

Indirect potable water reuse to face drought events in Barcelona city. Setting a monitoring procedure to protect aquatic ecosystems and to ensure a safe drinking water supply

The climate change and increasing anthropogenic pressures are expected to limit the availability of water resources. Hence, active measures must be planned in vulnerable regions to ensure a sustainable water supply and minimize environmental impacts. A pilot test was carried out in the Llobregat River (NE Spain) aiming to provide a useful procedure to cope with severe droughts through indirect water reuse. Reclaimed water was used to restore the minimum flow of the lower Llobregat River, ensuring a suitable water supply downstream for Barcelona. A monitoring was performed to assess chemical and microbiological threats throughout the water treatment train, the river and the final drinking water, including 376 micropollutants and common microbiological indicators. The effects of water disinfection were studied by chlorinating reclaimed water prior to its discharge into the river.Data showed that 10 micropollutants (bromodichloromethane, dibromochloromethane, chloroform, EDDP, diclofenac, iopamidol, ioprimid, lamotrigine, ofloxacin and valsartan) posed a potential risk to aquatic life, whereas one solvent (1,4-dioxane) could affect human health. The chlorination of reclaimed water mitigated the occurrence of pharmaceuticals but, conversely, the concentration of halogenated disinfection by-products increased. From a microbiological perspective, the microbial load decreased along wastewater treatments and, later, along drinking water treatment, ultimately reaching undetectable values in final potable water. Non-chlorinated reclaimed water showed a lower log reduction of E. coli and coliphages than chlorinated water. However, the effect of disinfection vanished once reclaimed water was discharged into the river, as the basal concentration of microorganisms in the Llobregat River was comparable to that of non-chlorinated reclaimed water. Overall, our study indicates that indirect water reuse can be a valid alternative source of drinking water in densely populated areas such as Barcelona (Catalonia - NE Spain). A suitable monitoring procedure is presented to assess the related risks to human health and the aquatic ecosystem.

Исследование качества протеинового батончика для спортивного питания с рыбными белковыми добавками

The quality of a new protein bar for sports nutrition, enriched with fish protein supplements, obtained by hydrolysis of fish scales, was investigated. The objects of the study were two types of protein supplements and the finished product. The paper explores the main indicators of the bar quality. The mass fraction of moisture, fat, and ash was determined according to GOST 5900-2014, GOST 31902-2012 and GOST 5901-2014, the amino acid composition was determined by ion-exchange chromatography, and the mineral composition was determined by atomic absorption spectrometry. The biological value of the additives’ protein and the finished product was determined by the calculation method, structural and mechanical characteristics – using a Brookfield CT3 texture analyzer, organoleptic indicators – on a 100-point scale, microbiological indicators – by standard methods. The peptide-protein and protein-mineral supplements contained 98.8% and 54.1% protein, respectively. The second supplement contained calcium and phosphorus, respectively, 22% and 12%. The amino acid composition of the bar showed the presence of all essential amino acids and glycine, proline, alanine, which are important for the athletes. The mass fraction of protein in the finished bar was 23.5%, which is adequate to 24% of its total energy value and corresponds to high-protein sports nutrition products according to GOST 34006-2016. It has been proven that a bar weighing 60 g is functional in terms of the content of calcium and phosphorus (satisfaction with the daily norm by 44% and 31.4%, respectively), vitamin E and dietary fiber (satisfaction by 22.4% and 58%). The organoleptic analysis revealed its positive perception in all respects and the absence of a fishy aftertaste; the total scoring of the product was 85.75 points. Rational values of rheological parameters were established: limiting shear stress 20–25 kPa, adhesion 200–220 mJ, penetration number 4.7–5.2 mm, elastic modulus 0.08–0.1. According to the results of microbiological studies, the shelf life of protein bars was 28 days.

Microbiological indicators as sensitive indicators in the assessment of areas contaminated by heavy metals

Context As one of the world’s largest mining spills, the Aznalcóllar pyrite mine accident in Spain in 1998 resulted in ~45 km2 of agricultural soils polluted by arsenic and heavy metals. The Guadiamar Green Corridor (GGC) helped with soil remediation but residual pollution is still detected 20 years later. Aims Several methodologies based on chemical indicators have been used to assess the recovery of these areas. However, simple microbiological indicators (e.g. microbial activity and biomass, and metabolic quotient (qCO2)) are yet to be uesed. The aim of this study was to evaluate areas contaminated by arsenic and heavy metals using microbiological indicators of soil quality. Methods We used a systematic random sampling design to collect soil samples from two soil groups with different recovery trajectories. We analysed the total and water-soluble concentrations of arsenic (As), lead (Pb), copper (Cu), and zinc (Zn), the main soil properties, and bioassays including microbial activity and biomass, and metabolic quotient or microbial stress (qCO2). Key results Twenty years after the accident, soils with As and Pb concentrations that consistently exceeded regulatory levels had altered soil microbial biomass and functioning. Although overall rates of microbial respiration were not significantly different between polluted soils, microbial biomass was lower and qCO2 was higher in the more polluted than in less polluted soils. Conclusions The metabolic quotient and microbial biomass are sensitive indicators in the monitoring over time of soil polluted by arsenic and heavy metals. Implications Microbial indicators must be considered in the assessment of potential ecotoxicity and in the evaluation of soil biological properties influencing soil recovery in the long term.

Development of technology of gingerbread products enriched with raw materials of the Far Eastern region

Providing the population with high-quality food products enriched with physiologically significant nutrients at an affordable price is a priority task of the state policy and the government of the Russian Federation. The article shows the possibility of expanding of the range of gingerbread products enriched with natural raw materials of the Far Eastern region. The technologies of the production of berry powders recommended as functional ingredients have been developed. It has been established that the replacement of flour with powder from Kamchatka bilberry pomace in the amount of 5.5% or powder from common barberry in the amount of 7.5%, soy deodorized flour of the highest grade in the amount of 5% and food additive from Daurian larch wood "Arabinogalactan" in the amount of not more than 3% in gummy gingerbread recipe contributes to obtaining a product that meets the requirements of GOST 15810-2014 and safety indicators of the requirements of the Technical Regulations of the Customs Union (TR CU) 021/2011 "On Food Safety". Laboratory studies of the developed gummy gingerbread have proved compliance with the requirements of regulatory documents for organoleptic, physicochemical and microbiological indicators. The technology development and the use of berry powders in the production, which make it possible to enrich traditional food products is of practical interest. The expediency of using regional raw materials to expand the range of flour confectionery products of increased nutritional and biological value has been proved.

Assessment of the safety and quality of turkey meat depending on the methods of keeping

The research was carried out on broiler-type turkeys of the BUT-9 cross in the Republic of Mari El and private subsidiary farms. In accordance with our goals and objectives, the objects of research were blood and blood serum, red and white meat obtained from broiler turkeys of industrial type of cultivation and home type of cultivation. The study of turkey meat included: organoleptic studies, tasting evaluation of meat, determination of chemical and physico-chemical composition, energy value, amino acid and fatty acid composition of meat, microbiological studies, determination of meat freshness indicators The quality and nutritional value of meat was studied (anatomical cutting of turkey carcasses, chemical and physico-chemical indicators of meat, amino acid and fatty acid composition of meat, micro content- and macronutrients) the preservation of turkey meat of different content systems at t +2 +4 °C (organoleptic, chemical, physico-chemical, microbiological indicators) and at t-12-14 °C (organoleptic, chemical, physico-chemical indicators). White and red meat of the domestic content system is less stable during storage at t+2+4 °C according to such indicators as: pH, acidity/oxidizability coefficient, LFA, amino-ammonia nitrogen, protein, microbiological parameters (KMAFAnM). The study of the storage conditions of turkey meat at t-12-14 °C ensures the safety of meat a home growing system for 72 hours, and an industrial one for 96 hours. The meat of all growing systems, both females and males, had high stability during freezing storage. Study of storage conditions at t -12-14°C ensures the preservation of meat for 6 months without reducing the freshness indicators.

Prospects of using quail products in the production of meat souffle

This research is focused on the development of a recipe composition for meat soufflé enriched with "Iodselen" bioactive additive. The objects of the study were as follows: experimental samples of meat soufflé, obtained according to 3 recipe options with different ratios of the main raw materials, wt.%: quail meat from 80.0 to 90.0, rendered quail fat from 10 to 20. By way of comparison, a meat product has been chosen, the recipe of which is described in patent No. 2726428. As a part of the research, the choice of components of the recipe composition of meat soufflé has been scientifically substantiated, and its quality has been assessed according to organoleptic, physicochemical and microbiological indicators. During the tasting assessment, it has been found that the meat soufflé is characterized by high quality indicators. The highest score has been obtained by sample according to option 3 (5.0 points), in which meat and rendered fat are presented in the following ratio of 85.0:15.0 wt.%. The highest protein content has been measured to be in the meat soufflé according to option 4 which is 28.7%, while the amount of fat in the product obtained according to option 2 was 10.4%, which is 14.7% and 26.6% more than in samples 3 and 4. This is directly related to the introduction of different amounts of basic raw materials into the recipe. It has been found that sample 2 is possessed by the highest energy value of 252.8 kcal (1.058.4 kJ), to the recipe of which 80.0 wt.% of raw meat and 20.0 wt.% of rendered fat were added. However, according to organoleptic assessment, sample 3 is more balanced. The functional orientation of the developed meat product has been proven, so the introduction of 0.06% of “Iodselen” into the soufflé satisfies the body’s daily need for iodine, which is 150 mcg/day, and selenium, which is 55 mcg/day for women and 70 mcg/day for men, for adults (over 18 years old).