Microbiological Environment Research Articles

Ameliorative Effects of Camel Milk and Fermented Camel Milk on Acute Alcoholic Liver Injury

Probiotics, which are prevalent in camel milk (CM) and naturally fermented camel milk (FCM), can regulate the intestinal ecological structure to alleviate alcoholic liver disease (ALD) through the “gut–liver” axis. The protective effects and mechanisms of CM and FCM interventions on alcohol-induced acute liver injury were investigated by combining the behavior observed in rats following alcohol exposure. The results revealed that CM and FCM effectively controlled the increased levels of alcohol-induced ALT, AST, TG, MDA, and proinflammatory cytokines. Alcohol-induced oxidative depletion of hepatic CAT, GPX, GSH, and ALDH was reversed, diminishing lipid accumulation, ameliorating severe pathological damage, increasing antioxidant capabilities, and postponing oxidative stress. Additionally, the abundance of the phylum Bacteroidota (which reduces the F/B ratio); the family Prevotellaceae; the genera Clostridia_vadinBB60_group, parabacteroides, Alloprevotella, and Prevotellaceae_UC_G001; the gastrointestinal barrier; and the microbiological environment was increased. The steroid hormone biosynthesis pathway was altered to reduce alcohol-induced predominant steroid metabolites such as 17-hydroxyprogesterone, cortisol, and dehydroepiandrosterone, preventing alcoholic liver impairment. Taken together, CM could be a therapeutic dietary supplement for preventing alcoholic liver injury by ameliorating the intestinal ecology and hepatic metabolism.

The Time Has Come: The Case for Initiating Pilot Clinical Trials of Pig Kidney Xenotransplantation.

In vitro studies indicate that kidney transplantation from gene-edited pigs in which expression of all three of the known glycan xenoantigens has been deleted may be more challenging in nonhuman primates (NHPs) than it will be in human recipients. Furthermore, pig-to-human xenotransplantation offers several other advantages - (i) the patient can communicate with the surgical team; (ii) recipient microbiological monitoring and environment will be clinical-grade; and (iii) sophisticated graft monitoring and imaging techniques, (v) therapeutic interventions, e.g., dialysis, plasmapheresis, and (v) intensive care can be deployed that are not easily available in NHP laboratory models. We suggest, therefore, that progress to develop safe, informative human clinical trials will be accelerated if pilot clinical cases are initiated. The selection of patients for kidney xenotransplantation can include those who are at high risk of dying imminently, e.g., those experiencing increasing vascular access challenges with no realistic alternative therapy available, and those who have been accepted onto the waitlist for an allograft, but who are unlikely ever to receive one. Patients with an increased risk of dying include those with (i) age >60 years, (ii) blood groups O or B, and (iii) diabetic nephropathy. UNOS data indicate that an average of 25 patients on the kidney waitlist in the USA die or are removed from the list every day (i.e., >9,000 each year). Given the improved xenograft survival observed in preclinical studies, we suggest that it is time to plan a small pilot clinical trial for healthy dialysis patients who understand the risks and potential benefits of kidney xenotransplantation.

Investigating the synergistic effects of biochar, trans-zeatin riboside, and Azospirillum brasilense on soil improvement and enzymatic activity in water-stressed wheat

BackgroundWater stress is a major danger to crop yield, hence new approaches to strengthen plant resilience must be developed. To lessen the negative effects of water stress on wheat plants, present study was arranged to investigate the role of synergistic effects of biochar, trans-zeatin riboside (t-ZR), and Azospirillum brasilense on soil improvement and enzymatic activity in water-stressed wheat.ResultsIn a three-replication experiment comprising of four treatments (T0: Control, T1: Drought stress (DS), T2: DS + t-ZR with biochar, T3: DS + A. brasilense with biochar), we observed notable improvements in soil quality and enzymatic activities in water-stressed wheat plants with the application of t-ZR and A. brasilense with biochar. In drought stress, Treatment having the application of A. brasilense with biochar performs best as compared to the other and significant increased the enzymatic activities such as peroxidase (7.36%), catalase (8.53%), superoxide dismutase (6.01%), polyphenol oxidase (14.14%), and amylase (16.36%) in wheat plants. Different enzymatic activities showed different trends of results. Soil organic C, dissolved organic C, dissolved organic N also enhanced 29.46%, 8.59%, 22.70% respectively with the application of A. brasilense with biochar under drought stress condition.ConclusionsThe synergistic action of A. brasilense and biochar creates an effective microbiological environment that supports essential plant physiological processes during drought stress. This enhancement is attributed to improved soil fertility and increased organic matter content, highlighting the potential of these novel strategies in mitigating water stress effects and enhancing crop resilience.

Impact of clear aligners on plaque and periodontal health

In recent years, clear aligners have become a well-liked substitute for traditional braces in orthodontic treatment. Examining the effects of clear aligners on periodontal health and plaque accumulation is the goal of this review. To examine pertinent studies that have been published up to this point, a comprehensive evaluation of the literature was carried out. The review includes research on the microbial alterations in tooth plaque composition that occur during clear aligner therapy and the subsequent impact on periodontal tissues. Clear aligners and traditional braces are also compared with respect to how they affect gingival health, periodontal disease prevalence, and oral hygiene practices.In terms of plaque control, the results imply that transparent aligners offer special benefits and challenges. An important factor influencing the microbiological environment in the oral cavity is patient compliance, the type of aligner used, and the subtleties of its design. Moreover, the effect of orthodontic therapies on periodontal health is explored, providing insight into possible associations between the use of clear aligners and periodontal results. By underlining the necessity of individualized oral hygiene management strategies during clear aligner therapy, this review offers orthodontic practitioners’ insightful information. It highlights the need of maintaining periodontal health throughout orthodontic treatment and stresses the necessity of a multidisciplinary approach to optimize patient outcomes. In summary, this comprehensive review provides an overview of the most recent findings regarding the impact of clear aligners on plaque and periodontal health. By teaching clinicians, researchers, and patients about the subtleties of oral hygiene care during orthodontic treatment, the findings are meant to direct the development of effective strategies for maintaining good periodontal health in patients getting clear aligner therapy.

Predictive and Prognostic Biomarkers in Patients With Mycosis Fungoides and Sézary Syndrome (BIO-MUSE): Protocol for a Translational Study.

Cutaneous T-cell lymphoma (CTCL) is a rare group of lymphomas that primarily affects the skin. Mycosis fungoides (MF) is the most common form of CTCL and Sézary syndrome (SS) is more infrequent. Early stages (IA-IIA) have a favorable prognosis, while advanced stages (IIB-IVB) have a worse prognosis. Around 25% of patients with early stages of the disease will progress to advanced stages. Malignant skin-infiltrating T-cells in CTCL are accompanied by infiltrates of nonmalignant T-cells and other immune cells that produce cytokines that modulate the inflammation. Skin infection, often with Staphylococcus aureus, is frequent in advanced stages and can lead to sepsis and death. S. aureus has also been reported to contribute to the progression of the disease. Previous reports indicate a shift from Th1 to Th2 cytokine production and dysfunction of the skin barrier in CTCL. Treatment response is highly variable and often unpredictable, and there is a need for new predictive and prognostic biomarkers. This prospective translational study aims to identify prognostic biomarkers in the blood and skin of patients with MF and SS. The Predictive and Prognostic Biomarkers in Patients With MF and SS (BIO-MUSE) study aims to recruit 120 adult patients with MF or SS and a control group of 20 healthy volunteers. The treatments will be given according to clinical routine. The sampling of each patient will be performed every 3 months for 3 years. The blood samples will be analyzed for lactate dehydrogenase, immunoglobulin E, interleukins, thymus and activation-regulated chemokine, and lymphocyte subpopulations. The lymphoma microenvironment will be investigated through digital spatial profiling and single-cell RNA sequencing. Microbiological sampling and analysis of skin barrier function will be performed. The life quality parameters will be evaluated. The results will be evaluated by the stage of the disease. Patient inclusion started in 2021 and is still ongoing in 2023, with 18 patients and 20 healthy controls enrolled. The publication of selected translational findings before the publication of the main results of the trial is accepted. This study aims to investigate blood and skin with a focus on immune cells and the microbiological environment to identify potential new prognostic biomarkers in MF and SS. ClinicalTrials.gov NCT04904146; https://www.clinicaltrials.gov/study/NCT04904146. DERR1-10.2196/55723.

Managing the Risk of Foodborne Infections in Pediatric Patients with Cancer: Is the Neutropenic Diet Still an Option?

Infections pose a significant threat to morbidity and mortality during treatments for pediatric cancer patients. Efforts to minimize the risk of infection necessitate preventive measures encompassing both environmental and host-focused strategies. While a substantial number of infections in oncologic patients originate from microorganisms within their native microbiological environment, such as the oral cavity, intestines, and skin, the concrete risk of bloodstream infections linked to the consumption of contaminated food and beverages in the community cannot be overlooked. Ensuring food quality and hygiene is essential to mitigating the impact of foodborne illnesses on vulnerable patients. The neutropenic diet (ND) has been proposed to minimize the risk of sepsis during neutropenic periods. The ND aims to minimize bacterial entry into the gut and bacterial translocation. However, a standardized definition for ND and consensus guidelines for specific food exclusions are lacking. Most centers adopt ND during neutropenic phases, but challenges in achieving caloric intake are common. The ND has not demonstrated any associated benefits and does not ensure improved overall survival. Consequently, providing unified and standardized food safety instructions is imperative for pediatric patients undergoing hematopoietic cell transplantation (HCT). Despite the lack of evidence, ND is still widely administered to both pediatric and adult patients as a precautionary measure. This narrative review focuses on the impact of foodborne infections in pediatric cancer patients and the role of the ND in comparison to food safety practices in patients undergoing chemotherapy or HCT. Prioritizing education regarding proper food storage, preparation, and cooking techniques proves more advantageous than merely focusing on dietary limitations. The absence of standardized guidelines underscores the necessity for further research in this field.

How Organic Mulching Influences the Soil Bacterial Community Structure and Function in Urban Forests.

Urban forest soil is often disturbed by frequent human activity. Organic mulching is effective for improving soil quality; however, the effects of organic mulching on soil bacterial communities in urban forests are still largely unexplored. This study evaluated how organic mulching changed the urban forest soil bacterial community through an incubation experiment. Four treatments were applied: (1) no organic mulch (CK); (2) wood chips alone (5 g, Mw); (3) wood compost alone (5 g, Mc); and (4) wood chips + wood compost (This mulch was divided into two layers, i.e., the upper layer of wood chips (2.5 g) and the lower layer wood compost (2.5 g, Mw+c).) We found significant differences in the soil physicochemical properties under organic mulching after incubation. Overall, organic mulching can alter soil bacterial community structure. Soil alkali-hydrolyzable nitrogen, soil organic carbon, soil total nitrogen, and carbon-nitrogen ratio were the main factors affecting soil microbial community structures. Soil bacterial groups under organic mulching treatments mainly acted on the C and N cycling of functional pathways in soil. This study suggests that organic mulching could maintain the development of soil bacteria, which establishes a theoretical foundation for enhancing the microbiological environment of urban forest soils.

Isolation, identification, and mechanism analysis of plant growth-promoting rhizobacteria in tobacco.

Plant growth, crop yield, and pest and disease control are enhanced by PGPR (Plant growth promoting rhizobacteria), which are beneficial microorganisms found in a close symbiosis with plant roots. Phytohormones are secreted, nutrient uptake is improved, and soil properties along with the microbiological environment are regulated by these microorganisms, making them a significant focus in agricultural research. In this study, the efficient PGPR strain T1 was isolated and screened from tobacco inter-root soil, and identified and confirmed by ITS sequencing technology. Tobacco growth indicators and soil property changes were observed and recorded through potting experiments. The activities of key enzymes (e.g., sucrase, catalase, urease) in soil were further determined. High-throughput sequencing technology was utilized to sequence the soil microbial community, and combined with macro-genomics analysis, the effects of T1 strain on soil microbial diversity and metabolic pathways were explored. Following the application of T1, significant improvements were observed in the height, leaf length, and width of tobacco plants. Furthermore, the physical and chemical properties of the soil were notably enhanced, including a 26.26% increase in phosphorus availability. Additionally, the activities of key soil enzymes such as sucrase, catalase, and urease were significantly increased, indicating improved soil health and fertility. Comprehensive joint microbiomics and macrogenomics analyses revealed a substantial rise in the populations of beneficial soil microorganisms and an enhancement in metabolic pathways, including amino acid metabolism, synthesis, and production of secondary metabolites. These increase in beneficial microorganisms and the enhancement of their metabolic functions are crucial for plant growth and soil fertility. This study provides valuable references for the development of innovative microbial fertilizers and offers programs for the sustainable development of modern agriculture.

F-free etching and ingenious construction of hydrogel layer-prepared MXene membranes for oily wastewater separation.

A strategy for the preparation of hydrogel layer MXene membranes by an F-free method was proposed. It maintained high permeance (2686.1 L m-2 h-1 bar-1) and separation efficiency (99.99%) even after 300 min of emulsion separation. The membrane resisted harsh chemical and microbiological environments and efficiently treated actual oily wastewater.

Modeling of culture conditions by culture system, glucose and propionic acid and their impact on metabolic profile in IPEC-J2.

The microbiological environment and their corresponding secreted metabolite spectrum are an essential modulator of the enterocyte function, effecting the whole organism. Intestinal porcine jejunal epithelial cell line (IPEC-J2) is an established in vitro model for differentiation of enterocytes in different cell culture models. An improved oxygen supply seems to be the main reason for differentiation in an air-liquid-interface culture, but this has not yet been conclusively clarified. In this context, the nutrition of the cell and its influence on the metabolism is also of crucial importance. The interest in short-chain fatty acids (SCFAs) has grown steadily in recent years due to their clinical relevance in certain diseases such as multiple sclerosis and other inflammatory diseases, but not much is known of FFAR2 and FFAR3 (free fatty acid receptor 2 and 3) in pigs. We want to address the questions: 1. about the distribution of FFAR2 and FFAR3 in vivo and in vitro in sus scrofa 2. whether there is an influence of propionic acid, glucose content and cultivation on metabolism of enterocytes? The morphological analysis of FFAR2 and FFAR3 in vivo was investigated through immunostaining of frozen sections of the porcine gut segments jejunum, ileum and colon. Both receptors are expressed along the gut and were found in the smooth muscle cells of the tunica muscularis and lamina muscularis mucosae. Furthermore, a high expression of FFAR2 and a low expression of FFAR3 in the enteric nerve system was also observed in jejunum, ileum and colon of sus scrofa. In addition, FFAR2 and FFAR3 within the vessels was investigated. FFAR3 showed a strong expression on endothelial cells of veins and lymphatic vessels but was not detectable on arteries. Furthermore, we demonstrate for the first time, FFAR2 and FFAR3 in IPEC-J2 cells on RNA- and protein level, as well as with confocal microscopy. In addition, ENO1 and NDUFA4 were investigated on RNA-level in IPEC-J2 cells as 2 important genes, which play an essential role in metabolism. Here, NDUFA4 is detected in the model animal sus scrofa as well as in the porcine cell line IPEC-J2. A potential impact of propionic acid and/or glucose and/or cultivation method on the metabolism of the cells was tested with the Seahorse analyzer. Here, a significant higher ECAR was observed in the SMC than in the OCR. In summary, we were able to show that the cultivation system appears to have a greater influence than the medium composition or nutrition of the cells. However, this can be modulated by incubation time or combination of different SCFAs.

Effects of water and fertilizer regulation on soil physicochemical properties, bacterial diversity and community structure of Panax notoginseng

Improper irrigation and fertilization can result in soil compaction, solidification, and acidification, consequently disrupting the equilibrium of the soil microbial community. The objective was to enhance the microbial environment in Panax notoginseng soil, augment bacterial diversity, increase community abundance, and establish a scientific and theoretical foundation for Panax notoginseng cultivation. Panax notoginseng field planting experiment was carried out from 2019 to 2021, involving two controlled variables: irrigation (W) and fertilizer application (F). Three irrigation levels were established, denoted as W1 (0.6 FC), W2 (0.75 FC), and W3 (0.9 FC). A total of 1440 kg•hm−2 of soluble organic fertilizer was applied throughout the entire growth cycle, with varying proportions allocated to each growth stage. Four fertilization levels were designated as F1 (rooting stage: seedling stage: flowering stage: fruiting stage = 25 %: 25 %: 25 %: 25 %), F2 (20 %: 25 %: 30 %: 25 %), F3 (15 %: 30 %: 30 %: 25 %), and F4 (10 %: 40 %: 20 %: 30 %), total 12 treatments. Non-irrigated and non-fertilized CK treatments were employed as control. Soil physicochemical attributes, bacterial diversity, community structure, and functional characteristics across various water and fertilizer treatments were comprehensively assessed utilizing the quotient-weighted TOPSIS method. The results showed that coupled irrigation and fertilization treatments had a significant impact on various soil parameters of Panax notoginseng, including total nitrogen, nitrate nitrogen, ammonium nitrogen, organic carbon, pH, water content, and bacterial alpha diversity (P < 0.05). Moreover, coupled irrigation and fertilization treatments had a highly significant influence on soil water content during the rooting stage, seedling stage, and fruiting stage (P < 0.01). Total nitrogen, nitrate nitrogen, ammonium nitrogen, water content, and pH emerged as the primary environmental factors significantly shaping the structure of the soil bacterial community. In the W2F4 treatment, soil total nitrogen, organic carbon content, pH, Chao1, Shannon index, and functional abundance in categories such as chemoheterotrophy, aerobic chemoheterotrophy, nitrogen fixation, nitrate reduction, nitrogen respiration, and nitrate respiration were notably higher compared to other water-fertilized treatments. Furthermore, the W2F4 treatment ranked first in the TOPSIS comprehensive evaluation. Consequently, the W2F4 treatment emerged as the optimal irrigation-fertilization strategy for enhancing soil microbial activity and fertility in Panax notoginseng cultivation. This study establishes a theoretical foundation for enhancing the microbiological environment of Panax notoginseng soils, augmenting soil bacterial diversity, increasing community abundance, and ultimately elevating soil fertility for the efficient and high-quality cultivation of Panax notoginseng.

Soil Bacterial Community Structure and Function Prediction of Millet/Peanut Intercropping Farmland in the Lower Yellow River

The objective of this study was to explore the microecological variability in farmland soil fertility in response to millet-peanut intercropping patterns by clarifying the effects of millet-peanut 4:4 intercropping on soil bacterial community structure and its diversity, as well as to provide a reference basis for promoting ecological restoration and arable land quality improvement in the lower Yellow River farmland. The Illumina MiSeq high-throughput sequencing technology and QIIME 2 platform were used to analyze the differences in bacterial community composition and their influencing factors in five soils[sole millet (SM), sole peanut (SP), intercropping millet (IM), intercropping peanut (IP), and millet-peanut intercropping (MP)] and to predict their ecological functions. The results showed that the α-diversity of intercropping soil bacterial communities differed from that of monocropping, though not significantly, whereas the β-diversity was significantly different (P<0.05). A total of 7081 ASVs were obtained from all soil samples, classified into 34 phyla, 109 orders, 256 class, 396 families, 710 genera, and 1409 species, of which 727 ASVs were shared, accounting for 24.5% to 27.8% in five soil species. The bacterial communities of millet-peanut intercropping and its monocropping soils were similar in phylum composition but varied in relative abundance. All five soils were dominated by the Actinobacteria, Proteobacteria, Acidobacteria, and Chloroflexi, with a relative abundance of 79.40%-81.33%. Soil organic carbon and alkaline nitrogen were the most important factors causing differences in the structures of the five soil bacterial communities at the phylum and genus levels, respectively. The PICRUSt functional prediction revealed that the relative abundance of primary functional metabolism was the largest (78.9%-79.3%), and the relative abundance of secondary functional exogenous biodegradation and metabolism fluctuated the most (CV=3.782%). In terms of the BugBase phenotype, the relative abundance of oxidative stress-tolerant bacteria increased in intercropping millet or peanut soils compared to that in the corresponding monocultures and significantly increased in intercropping millet soils compared to that in sole millet (P<0.05). Oxidative stress-tolerant, Gram-positive, and aerobic phenotypes were highly significantly positively correlated with each other (P<0.01), and all three showed highly significant negative correlations with potential pathogenicity and Gram-negative and anaerobic phenotypes (P<0.01). This showed that millet-peanut intercropping resulted in differences in soil bacterial community diversity, abundance, and metabolic functions and the possibility of reducing the occurrence of potential soil diseases. It can be used to regulate the soil microbiological environment to promote ecological restoration and sustainable development of farmland in the lower Yellow River.

The Effect of Capsaicin on Growth Performance, Antioxidant Capacity, Immunity and Gut Micro-Organisms of Calves.

Capsaicin is the active ingredient of the red pepper plant of the genus Capsicum. The aim of this study was to investigate the effects of different doses of capsaicin on growth performance, antioxidant capacity, immunity, fecal fermentation parameters and gut microbial composition in nursing calves. Twenty-four newborn Holstein calves were randomly assigned to three treatment groups, which each consisted of eight calves. The milk replacer was supplemented with 0, 0.15 or 0.3 mL/d of capsaicin in each of the three treatment groups. During the 4-week experiment, intake was recorded daily, body weight and body size parameters were measured at the beginning and end of the trial and serum samples and rectal fecal samples were collected at the end of the trial to determine serum parameters, fecal fermentation parameters and fecal microbiome compartments. The results showed that both doses of capsaicin had no negative effect on the growth performance or the fecal fermentation parameters of calves, and the higher dose (0.3 mL/d) of capsaicin significantly improved the antioxidant capacity and immunity of calves. The calves in the high-dose capsaicin-treated group had lower fecal scores than those recorded in the control group. High doses of capsaicin increased glutathione antioxidant enzyme, superoxide dismutase, immunoglobulin A, immunoglobulin G, immunoglobulin M and interleukin-10 levels and decreased malondialdehyde and bound bead protein levels. In addition, capsaicin regulated the gut microbiota, reducing the abundance of diarrhea-associated bacteria, such as Eggerthella, Streptococcus, Enterococcus and Enterobacteriaceae, in the gut of calves in the treated group. Therefore, high doses of capsaicin can improve the antioxidant and immune capacity of calves without affecting growth performance, as well as improve the gut microbiological environment, which enables the healthy growth of calves.

P-552 Pilot study to determine efficacy of L. rhamnosus BPL205, L. plantarum BPL207 and L.crispatus BPL209 in normalizing the vaginal environment (study PROSALVAG)

Abstract Study question Can the use of orally L.rhamnosus PBL205, L. plantarum BPL207 and L.crispatus BPL209 reestablish the vaginal environment? Summary answer The use of oral probiotics changes the infectious microbiological vaginal environment to a healthy one, consequently the vaginal pH is reestablished. What is known already The normal flora of the reproductive tract includes a variety of Lactobacillus species that promote a healthy environment for the embryo during the preimplantation period, so that Lactobacillus spp. are considered to promote ideal environment for implantation. Live birth rate correlates directly with the presence of Lactobacillus and inversely with the existence of bacterial vaginosis. Alterations of the vaginal flora such as bacterial vaginosis are related to an increased risk of miscarriage. It has been shown that women with an unbalanced vaginal microbiome are 1.4 times less likely to achieve pregnancy after IVF treatment compared to women with normal microbiome. Study design, size, duration Prospective pilot study to assess the effect of 10 weeks of use of an oral probiotic containing Lactobacillus rhamnosus BPL205, Lactobacilus plantarum BPL 207 and Lactobacillus crispatus BPL209 supplemented with vitamin D, in vaginal health reestablishment. 21 women assisting to a fertility clinic and with indication of vaginal microbiota restoration and low number of vaginal Lactobacillus were recruited. After 5 and 10 weeks of supplementation, the reestablishment of vaginal health was study. Participants/materials, setting, methods Women with indication of vaginal microbiota restoration, low number of vaginal Lactobacillus and with the informed consent signed, were asked to take a nutraceutical with probiotic and vitamin D once a day for 10 weeks. Cytology, vaginal pH and pain were analyzed at 5 and 10 weeks. In those patients with some kind of infection diagnosed the levels of TNF-α was analyzed after 5 weeks of probiotic use. Main results and the role of chance All patients started the study with vaginal pH &amp;gt; 4,5. After the use of oral probiotic containing Lactobacillus rhamnosus BPL205, Lactobacilus plantarum BPL 207 and Lactobacillus crispatus BPL209 supplemented with vitamin D (oral probiotic) during 5 weeks, 66,7% of the patients obtained a pH &amp;lt; 4,5. After the use of oral probiotic during 10 weeks all patients achieve a pH &amp;lt; 4,5. At the beginning of the study all patients result in yeast or vaginosis profile in their cytology, in except one. After 5 weeks with oral probiotic, 73,7% of them turns into a healthy vaginal flora essential to control pathogen proliferation (Döderlein flora: more of two hundred species with Lactobacillus predominance). After 10 weeks this percentage increase to 89,5%. There is a statistically significant reduction in Menstrual pain from an intensity of 6,1± 1,8 (mean ± standard deviation) to an intensity of 3,8 ± 3,5 at week 5 and to a 1,5 ± 3,0 at week 10 (p = 0,01 and P &amp;lt; 0,0001 respectively). In patients diagnosed with chronic endometritis at the beginning of the study (n = 5) TNF-α levels decrease from 8,14 ± 1,8 to 5,59 ± 2,3 after 10 weeks using the oral probiotic in study (p = 0,02). Limitations, reasons for caution The main limitation was the number of patients recluted. We have present the preliminary results of a study that continue recruiting patients in order to diminish the limitation of the study. Also, the number of parameters studied is being increased Wider implications of the findings The use of L.rhamnosus PBL205, L. plantarum BPL207 and L.crispatus BPL209 reestablish the healthy vaginal environment of women with an unhealthy ones. This allows better environmen for fertility and t to control pathogen proliferation. An improvement in inflammatory conditions in that patients with chronic endometritis is also observed. Trial registration number Not applicable

Stenotrophomonas pavanii DJL-M3 inoculated biochar stabilizes the rhizosphere soil homeostasis of carbendazim-stressed rice

Plant–microbe interactions have been effectively used in phytoremediation to reduce agrochemical contamination of crops and soils, but little information is available regarding the general effect of such association on rhizosphere soil homeostasis. In this study, we immobilized Stenotrophomonas pavanii DJL-M3, a carbendazim (CBZ)-degrading endophyte, in rice husk-derived biochar to control fungicide residue in the rice microenvironment. The influence of biochar inoculated with strain DJL-M3 on rhizobacterial communities was also investigated, including activity and fundamental function predictions. An adsorption kinetics test showed that strain DJL-M3 slowed the adsorption rate slightly without sacrificing the adsorption capacity of rice-husk biochar on CBZ. Immobilization in biochar helped S. pavanii DJL-M3 to establish an ecological niche in rhizosphere soils. This process significantly reduced CBZ levels in rice and rhizosphere soil while maintaining stable heterotrophic microbial respiration and carbon (C) metabolic activity. Soil amendment with the strain DJL-M3–biochar composite resulted in relatively little disturbance of fundamental soil functions, such as nitrogen (N) and sulfur (S) cycling, which explained the better plant growth and higher soil fertility observed with CBZ contamination. Overall, the combination of biochar and S. pavanii DJL-M3 demonstrated the potential to safeguard the microbiological environment of rice.

Methodological approaches for studying the microbiological environment of drinking water distribution systems

Methodological approaches for studying the microbiological environment of drinking water distribution systems

Investigation of the microbiological environment in the temple where cultural assets are stored based on the treasure Hanging Painting of Anguksa Temple (The Vulture Peak Assembly) and storage chest analysis

Investigation of the microbiological environment in the temple where cultural assets are stored based on the treasure Hanging Painting of Anguksa Temple (The Vulture Peak Assembly) and storage chest analysis

CO27 Evaluation of Antiseptics and the Microbiological Environment Regarding Complications of Phlebitis Caused by Peripheral Cannulas

CO27 Evaluation of Antiseptics and the Microbiological Environment Regarding Complications of Phlebitis Caused by Peripheral Cannulas

Influence of pregravid preparation on the structural reorganization of the endometrium in women with chronic endometritis in infertility

Aim. To reveal the peculiarities of structural reorganization of the endometrium in various variants of pregravid preparation in women with chronic endometritis (CE) and infertility.&#x0D; Materials and methods. We examined 90 women aged 1840 who were treated for CE. For all patients, examination with microbiological (cultural) examination of the cervical canal to be separated for aerobic and facultative anaerobic microorganisms with the determination of sensitivity to antibiotics, ultrasound examination (ultrasound) of the pelvic organs and morphological examination of endometrial biopsy samples before and after treatment. All women of the study group were divided into 2 subgroups. Patients of the 1st subgroup (n=30) received standard treatment for CE antibacterial and non-steroidal anti-inflammatory drugs; in the 2nd (n=60) an additional course of immunostimulating therapy with a bacterial lipopolysaccharide preparation was prescribed.&#x0D; Results. According to the cultural method, repeated cultures from the cervical canal after treatment turned out to be sterile more often in women of the 2nd subgroup. Ultrasound diffuse-focal changes in the subendometrial zone of the myometrium occurred in all patients before and after treatment. In the 2nd subgroup, after treatment, significant changes in the endometrium were noted in the form of an increase in its thickness and a 4-fold decrease in the number of hyperechoic inclusions in the basal layer, as well as the disappearance of the irregularity of the endometrial closure line. In the endometrial biopsies of women of the 2nd subgroup, a significant decrease in the specific area of the inflammatory infiltrate, the absence of foci of fibrosis, and an increase in the thickness of the vessel wall were noted.&#x0D; Conclusion. The addition of a bacterial lipopolysaccharide to the standard CE treatment regimen not only improves the microbiological environment of the female genital tract, but also significantly affects the morphofunctional state of the endometrium.

Sulfate-Reducing Bacteria in Patients Undergoing Fixed Orthodontic Treatment

ObjectivesThe oral microbiological environment may be implicated in the corrosion of orthodontic metals. This study aimed to examine the prevalence of sulfate-reducing bacteria (SRB) in orthodontic patients undergoing fixed appliance treatment. MethodsSixty-nine orthodontic and 69 healthy non-orthodontic participants were enrolled in the study. Supragingival and subgingivaloral biofilm were collected and tested for the presence of SRB. The DNA extraction, polymerase chain reaction (PCR), and 16sRNA Sanger sequencing method was performed from the SRB-positive samples. The sequenced PCR products were analysed and compared with databases to identify the bacterial genus. ResultsAmongst 69 orthodontic patients, characteristic black precipitates developed in 14, indicating the presence of iron sulfides which demonstrates the likelihood of SRB. Alternatively, 2 out of 69 showed the presence of SRB in healthy non-orthodontic participants (controls). Desulfovibrio spp was confirmed by analyses of 16sRNA sequencing, which revealed that the SRB prevalence was 20% in the examined participants with orthodontic appliances. ConclusionsThe prevalence of SRB was found to be significantly higher amongst orthodontic patients compared to non-orthodontic participants. Presence of stainless steel in the oral environment may have facilitated the colonisation of SRB.