Microbially Induced Calcite Precipitation Research Articles

Exploring fungal potential for microbial-induced calcite precipitation (MICP) in bio-cement production

IntroductionMicrobial-induced calcite precipitation (MICP) involves various microorganisms, such as bacteria, fungi, and algae. This study focuses on producing bio-cement using fungal species and selecting potential candidates isolated from alkaline soil of different regions of Punjab, namely, Majha, Malwa, and Doaba.MethodsThe selection of fungi isolates capable of bio-cement production involves several tests, including a urease assay and calcium precipitation. Isolates having high urease enzyme production and the ability to perform calcite precipitation are selected for instrumental analyses such as X-ray diffraction (XRD) and scanning electron microscopy (SEM). The isolates selected for further analysis are S1 (3) with 8.879 ± 2.94 µg/ml, S1 (18) with 8.421 ± 0.13 µg/ml, and S4 (1) with 10.057 ± 0.45 µg/ml urease activity and least free calcium ions that are 2.337 ± 0.5 µg/ml, 3.339 ± 0.5 µg/ml, and 4.074 ± 0.1 µg/ml respectively.Results and discussionCalcite precipitation is confirmed through XRD and field emission scanning electron microscopy (FESEM). XRD images showing calcite precipitation with sharp crystalline peaks for S1 (3), S1 (18), and S4 (1) are shown. The calcite precipitation is evident in the micrographs of FESEM. These combined results confirm the potential of urease-positive fungi to facilitate calcite production, which could lead to bio-cement development in future research.

Behaviour and mechanism of cadmium immobilization in contaminated soil by calcium carbide residue-enhanced MICP

A novel technique that couples microbially induced calcite precipitation (MICP) and calcium carbide residue (CCR) is proposed for immobilizing Cd2+ in contaminated soil. The properties and mechanism of CCR-enhanced MICP were investigated through a series of experimental analyses considering factors such as heavy metal concentration, curing time, and the effect of Ca2+. The unconfined compressive strength (UCS) increased with increasing curing time and reached a maximum value at 28 d, and the leaching concentration of Cd2+ decreased and tended to level off with increasing curing time. The addition of CCR enhanced the immobilization performance of Cd2+ through the MICP method, resulting in UCSs that were 3.8–4.2 times those of samples without CCR and leaching concentrations of Cd2+ that were 38.9–69.2% lower at a curing time of 28 d. The addition of Ca2+ to cementation solutions further improved the immobilization effectiveness, resulting in the UCSs of the samples increasing by 18.7–49.8% and the leaching concentrations of Cd2+ decreasing by 11–40% CaCO3 and its hydration products can immobilize Cd2+ through coprecipitation, reducing its toxicity by converting weak acid-extractable cadmium into residual cadmium. Consequently, Sporosarcina pasteurii combined with CCR improved the UCS of the treated contaminated soil and greatly decreased cadmium migration.

Experimental Study of the Microstructural Characterization of Microbial Induced Calcite Precipitation (MICP) Bio-Cement Concrete

Experimental Study of the Microstructural Characterization of Microbial Induced Calcite Precipitation (MICP) Bio-Cement Concrete

Bhargavaea beijingensis a promising tool for bio-cementation, soil improvement, and mercury removal

Microbially Induced Calcite Precipitation (MICP) has emerged as a promising technique for bio-cementation, soil improvement, and heavy metal remediation. This study explores the potential of Bhargavaea beijingensis, a urease-producing bacterium, for these applications. Six ureolytic bacteria were isolated from calcareous bricks mine soil and screened for urease and calcite production. B. beijingensis exhibited the highest urease activity and calcite precipitation. Urease activity, calcite precipitation, sand solidification, heavy metal removal efficiency, and compressive strength were evaluated. It showed significant heavy metal removal efficiency, particularly highest for HgCl2. Mortar blocks treated with B. beijingensis or its crude enzyme exhibited improved compressive strength, suggesting its potential for bio-cementation. Crack remediation tests demonstrated successful crack healing in mortar blocks using the bacterium or its enzyme. This study identifies B. beijingensis as a novel and promising MICP agent with potential applications in bio-cementation, soil improvement, and heavy metal remediation. Hence, B. beijingensis diversified abilities prove superior performance compared to commonly used strains like Bacillus subtilis and Shewanella putrefaciens in bio-cementation applications. Its high urease activity, calcite precipitation, and heavy metal removal abilities make it a valuable candidate for sustainable and eco-friendly solutions in various fields.

Enhancing aeolian sand stability using microbially induced calcite precipitation technology

This study investigates the effectiveness of Microbially Induced Calcite Precipitation (MICP) technology in enhancing the stability of aeolian sand. Applying MICP to desert sand samples from Kashi, Xinjiang, the results demonstrated significant structural stability and erosion resistance in treated soils during wind erosion tests. Particularly after 14 days of treatment, the soil samples exhibited optimal wind erosion resistance and surface crust strength. Additionally, the formation of calcite significantly improved the soil’s penetration strength and wind erosion resistance, with SEM analysis confirming that calcite “bridges” between soil particles enhanced inter-particle bonding. Environmental impact assessments indicated that MICP technology is not only environmentally friendly but also effectively reduces the risk of soil environmental pollution. These findings validate the potential application of MICP technology in enhancing the stability and environmental adaptability of aeolian sand.

Influence of shear strength parameters on loose fine sand treated with coir fiber and microbially induced calcite precipitation

In recent years, there has been an increasing use of Microbially Induced Calcite Precipitation (MICP) technology in ground improvement. Adding fiber to bio-cemented sand leads to significant improvements in its properties. This paper examines how coir fiber and microbially induced calcite precipitation (MICP) might be used to improve the engineering qualities of fine sand. The sand was mixed with Coir fiber at varying Fiber Contents (FC) of 0.1%, 0.2%, 0.3%, 0.4%, and 0.5% by weight of sand. Additionally, varied Aspect Ratios (AR) of 45, 90, 136, 182, and 227 were considered. The mixture also included urease-producing bacteria (Sporosarcina Pasteurii), as well as solutions of urea and calcium chloride with 3M molarity. The untreated and treated sand samples were submitted to a Direct Shear Test (DST). The study found that the shear strength parameters, such as the angle of internal friction, increased by 1.1 times compared to standard fine sand. Additionally, the angle of internal friction (ϕ) of fiber-reinforced sand with MICP showed 1.4 times increase, while the cohesion values demonstrated a sixfold increase compared to MICP fine sand. It was determined that the optimal aspect ratio was 182, and the optimal fiber content was 0.3%. Moreover, the Scanning Electron Microscope (SEM) was utilized to examine the calcite formations present in the treated sand samples, and calcite precipitation was observed between the pores of the soils.

The impact of Microbially Induced Calcite Precipitation (MICP) on sand internal erosion resistance: A microfluidic study

Fine particle internal erosion involves the detachment, transport, and deposition of fine particles within soil, significantly impacting agriculture, engineering, and environmental protection. Microbially induced calcite precipitation (MICP) has proven to be an effective method for controlling internal erosion. To optimize MICP protocols for effective erosion control, understanding the microscopic mechanisms by which MICP reduces fine particle erosion is essential but remains unclear. In this study, microfluidic chip experiments were conducted to observe the characteristics of calcium carbonate crystals and fine particles before and after MICP reinforcement and erosion. Through quantitative analysis of the calcium carbonate produced by MICP and eroded fine particles, the effects of bacterial density, concentration of cementation solution, and flow rate on the efficiency of MICP in resisting soil internal erosion were investigated. In addition, three primary mechanisms by which MICP reduces fine particle erosion were identified. Firstly, in situ sand stabilization occurs when calcium carbonate generated by MICP bonds and encapsulates fine particles, forming larger particles that remain stable under erosive flow conditions. Secondly, regional sand stabilization is achieved as MICP-produced calcium carbonate crystals narrow or block the flow channels, preventing extensive migration of fine particles. Lastly, adjacent particle stabilization is facilitated by calcium carbonate crystals, which remain stable during water flow erosion and alter the erosion flow lines, creating zones adjacent to the crystals where fine particle movement is minimized. These findings provide a deeper understanding of the role of MICP in erosion control and can inform the development of more effective erosion mitigation strategies.

Physical property of MICP-treated calcareous sand under seawater conditions by CPTU

MICP (Microbially induced calcite precipitation), an environmentally friendly soil improvement technique, has great potential in ocean engineering due to its ability to promote the precipitation of calcium carbonate through microbial activity to enhance the engineering properties of geomaterials. In this study, piezocone penetration test (CPTU) is used to evaluate the effectiveness of MICP treatment in calcareous sand. The change of physical properties (relative density Dr and total unit weight γt) of MICP treated calcareous sand is investigated by conducting CPTU on the geomaterials prepared in a series of mini calibration chambers (25 cm × 50 cm). Results indicate that CPTU (tip stress, sleeve friction, and porewater pressure) measurements can be used to interpret the physical characteristics of calcareous sand treated with MICP under seawater conditions. Additionally, a relationship between CPTU measurements, physical parameters (relative density Dr and total unit weight γt) of MICP treated calcareous sand is proposed and calibrated. The findings of the research extend the implementation of in-situ testing techniques such as CPTU towards physical property evaluation of bio-treated geomaterials in ocean environment, and demonstrate the potential of scaling up MICP techniques for broader engineering application.

Effects of hexagonal boron nitride nanosheets on the biostabilization of recycled sands for geotechnical fill applications

Biostabilization techniques including plant enzyme induced calcite precipitation (EICP) and microbially induced calcite precipitation (MICP) represent promising and environmentally friendly methods for improving the engineering properties of recycled geomaterials for geotechnical fill applications. In this study, hexagonal boron nitride (h-BN) nanosheets were dispersed and utilized as nano-additives in the EICP and MICP reaction processes to facilitate the precipitation of calcium carbonate polymorphs (CaCO3) in washed recycled sands (RS) derived from construction and demolition (C&D) wastes for geotechnical fill applications. The investigation comprised a systematic range of biological and chemical microscopic and macroscopic experiments intending to stabilize the RS for geotechnical fill applications. The study results suggested that using h-BN nanosheets did not have a notable impact on bacterial growth or the functioning of bacterial and plant enzyme activity. In addition, it was observed that the optimal addition of h-BN nanosheet additives substantially increased CaCO3 precipitation by up to 34 % and 28 % in the EICP and MICP reaction processes respectively, which can be postulated to be due to the nanosheets acting as nucleation sites throughout the high surface area and negatively surface charge. Furthermore, the introduction of h-BN nanosheets led to a significant enhancement in the performance of unconfined compressive strength (UCS) of the stabilized RS through nanofillers and reinforcements, with improvements of up to 20 % and 13 % in the biostabilization of RS using the EICP and MICP after 10 treatment cycles. Detailed analyses, including Fourier-transform infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM) imaging, revealed that h-BN nanosheets, with a substantial surface area and effective chemical functional groups, interconnected with the CaCO3 mineral surface. Overall, the study suggests that integrating effective additives can improve the utilization of EICP and MICP in geotechnical fill applications, namely in road embankments and pavements, whilst providing both commercial viability and enhanced performance.

Mechanical properties of aeolian sand cemented via microbially induced calcite precipitation (MICP)

The cementation of desert aeolian sand is a key method to control land desertification and dust storms, so an economical, green and durable process to reach the binding between sand grains needs to be searched. The method based on the microbially induced calcite precipitation (MICP) appeared in recent years as a promising process that proved its efficiency. The feasibility of the MICP technique to treat aeolian sand composed by low clay content, fine particles, low water content and characterized by weak permeability was demonstrated in the present paper. The effects of initial dry density, cementation number and curing time on the permeability and strength of MICP-treated aeolian sand were investigated using permeability tests and unconfined compressive strength (UCS) tests. The microstructure of aeolian sand was observed by scanning electron microscopy (SEM) tests and X-ray diffraction (XRD), aiming to reveal the solidification principle of MICP. The tests result indicated that when the initial dry density and the cementation number rose, the hydraulic conductivity of aeolian sand decreased while the mechanical strength given by UCS values improved. When the initial dry density was 1.65 g/cm3, the curing time was 3 h and the cementation number reached 20, the hydraulic conductivity and UCS reached 0.00151 cm/s and 1050.30 kPa, respectively. With increasing curing time, the hydraulic conductivity first decreased, followed by an increase, while the UCS exhibited an up and then a downtrend. Furthermore, the correlation between UCS values and the CaCO3 content reached a high R2 value equal to 0.912, which confirmed that the cementation occurred in sandy material and governed the soil strengthening. Indeed, the calcium carbonate crystals observed by SEM and XRD enhanced the friction between particles when they wrapped around the sand grains surface, while carbonates reduced the soil permeability when filling the pores and sticking the sand particles together. Finally, the theoretical and scientific knowledge brought by the present study should help in managing sand in desert areas.

Numerical Investigation of Heterogeneous Calcite Distributions in MICP Processes

Microbially induced calcite precipitation (MICP) is a sustainable and environmentally friendly technology with applications in soil stabilization, concrete crack repair, and wastewater treatment. This study presents an improved Darcy-scale numerical model to simulate the MICP processes in heterogeneous porous media. It focuses on the effects of porosity heterogeneity, characterized by average porosity and correlation length, as well as injection strategies. Both average porosity and correlation length are critical factors influencing mass transport and calcite distribution during MICP treatment. An increase in average porosity leads to significant reductions in transport distance and total calcite mass. Notably, in the case of low averaged porosity, a larger correlation length results in more heterogeneous calcite distributions. However, there exists an upper threshold value of the initial averaged porosity (ϕ0=0.45) above which the heterogeneity of the calcite does not present clear dependence on the correlation length. Additionally, injection strategies significantly impact the consolidation effects. Compared to continuous injection, using the phased injection strategy can greatly improve the precipitated calcite area and mass due to its high utility and the efficiency of reactants.

Single-Particle Crushing Test of Coated Calcareous Sand Based on MICP

Calcareous sand is a crucial construction material for island and reef development and reinforcing it using Microbially Induced Calcite Precipitation (MICP) technology is a promising new method. This study employed 3D scanning technology to assess changes in the particle size and morphology of MICP-treated, coated calcareous sand particles. Single-particle crushing tests were conducted to analyze their crushing strength, crushing energy, crushing modes, and fragment fractal dimensions. The results indicated that MICP treatment significantly increased particle size, surface area, and volume, while reducing flatness. At a cementation solution concentration of 1 mol/L, both crushing strength and crushing energy were optimized. The coated particles exhibited three crushing modes: explosive crushing, mixed crushing, and splitting crushing. Thicker coatings led to a tendency for particles to break into larger fragments through the mixed and splitting crushing modes. Fractal analysis revealed that coating thickness directly affects the local crushing characteristics of the particles.

Sustainable Concreting: Optimization Modelling of the Strength Properties of Bio-Self Compacting Concrete Incorporating Sporosarcina Pasteurii, Calcined Clay and Limestone Powder

Sustainable concreting is prerequisite for infrastructural development in developing countries so as to meet up with the sustainable development goal of adequate mass housing and other critical infrastructure. Thus, research is ever ongoing aimed at developing cheaper and more durable concrete via the incorporation of bio-based by-products in concrete to improve its properties, as well as optimizing the quantities of these secondary materials for maximum and optimal concrete production. One such revolutionary concrete that is yet to find full application in the developing world is self-compacting concrete, because of the cost and attendant environmental effects. There is thus a need to arrive at optimal materials quantities that can maximize concrete properties without recourse to many trial and error experimentations that are both time and resources consuming. The application of modelling tools in concrete technology aids in the optimization of concrete constituents for optimal self-compacting concrete performance. This research uses optimization techniques to optimize the bacteria dosage as well as model the Compressive and Tensile strength properties of a calcined clay and Limestone powder blended ternary self-compacting concrete using sporosarcina pasteurii as Microbial induced calcite precipitation agent and calcium lactate as nutrient source. The Bacteria was incorporated into the concrete at a bacterial content of 1.5x108cfu/ml, 1.2x10 cfu/ml and 2.4x109cfu/ml corresponding to the McFarland turbidity scale of 0.5, 4 and 8 while the nutrient (calcium lactate) content was 0.5, 1.0 and 2.0% by weight of cement for each bacterial content. The Compressive strength and tensile strengths at 28 days were determined and the results used for both the model development, strength optimization and model validation, with the strengths as the dependent variable (y) and the bacterial content corresponding to a McFarland scale of and calcium lactate content as the independent variables, X1 and X2 respectively. The results show an improvement in the compressive strength from 32N/mm2 to 45.2N/mm2 at the optimal bacterial and nutrient content of 1.2x10 cfu/ml and 0.5% respectively, and tensile strength from 4.01N/mm2 to 5.0N/mm2. Also, the non-linear regression models proved adequate for optimizing the bacterial content for optimal self-compacting concrete performance. Journal of Engineering Science 15(1), 2024, 11-19

Numerical optimisation of microbially induced calcite precipitation (MICP) injection strategies for sealing the aquifer's leakage paths for CO2 geosequestration application

Carbon capture and storage (CCS) in deep geological aquifers has shown to be the most viable option for mitigating the greenhouse gas effect of carbon dioxide (CO2) at a large scale. However, the underground formations often possess discontinuities in the caprocks, leaking the stored CO2. Potential leakage paths, such as abandoned wells, have been growing due to excessively unplugged oil and gas exploration wells. The leakage of CO2 from these wells is a major concern, considering their negative impact on the environment and compromising CO2 storage efficiency. Recently, microbially induced calcite precipitation (MICP) technology has proven to be an effective and sustainable method for reducing the permeability of geomaterials. Nevertheless, the MICP process involves intricate interactions among bio-chemo-hydraulics (BCH) domains to comprehend the reactive transport of biochemicals. The complex nature of the MICP process poses difficulties in setting the biochemical injection durations for a particular target distance at the given injection rate. Given this, the present study developed a coupled numerical model and employed it as a workable tool for optimising MICP injections to plug the abandoned well connecting two deep geological aquifers. Following that, the study evaluated the leakage of CO2 using flow migration rates in the untreated and MICP-treated leaky aquifer. The study proposed a novel optimisation strategy for biochemical injections under near and far-field leakage conditions. The sensitivity of biochemical injection durations on the attached bacterial amount and permeability in the leak was also determined. The observations from the present study indicated a complete reduction in the CO2 migration rates from the abandoned well due to a reduced permeability after MICP, thereby indicating the efficacy of the proposed optimisation methodology. Further, a cost analysis of the MICP treatment indicated a rational application cost with the target distance compared to the detrimental effects of CO2 leakage.

Study on the improvement of grouting stone properties in coal mine goafs using combined denitrifying bacteria.

Grouting can effectively reduce residual deformation of coal mine goafs, but fly ash grouting materials suffer from poor flowability and slow early strength development. Microbially -induced calcite precipitation (MICP), with its high environmental compatibility and minimal disturbance to geotechnical bodies, effectively improves the injectability of grouting slurry in goafs. This study combined Castellaniella denitrificans and Sporosarcina pasteurii to induce calcite precipitation, preparing cement-fly ash slurry with varying water-solid ratios, solid ratios and denitrifying bacteria concentrations. The physical properties of the slurry and the mechanical properties of the grouted stone bodies under sealed curing conditions were measured. Results show that the dual-bacteria MICP improves stone body performance by enhancing cohesive, frictional and interlocking forces, so that the strength of the stone bodies cured by MICP increased rapidly within 7 days, and the strength reached the standard 2.03 MPa at 28 days under conditions of low solid ratio and high water-solid ratio, with the best compressive strength at a denitrifying bacteria concentration with an optical density of 0.8 at 600 nm wavelength. At a water-solid ratio of 1 : 1.2 and a solid ratio of 15%, initial and final setting times were 67.2 and 96 h, respectively, which prolonged the initial setting time and final setting time by nearly 70% and 110% compared with that of the slurry without MICP treatment, indicating that MICP enhances slurry fluidity, providing more time for grouting construction in goafs.

Bioremediation of uranium enriched coal fly ash based on microbially induced calcite precipitation

Coal fly ash (CFA) is an essential raw material in brickmaking industry worldwide. There are some coal mines with a relatively high content of uranium (U) in the Xinjiang region of China that are yet understudied. The CFA from these coal mines poses substantial environmental risks due to the concentrated uranium amount after coal burning. In this paper, we demonstrated a calcifying ureolytic bacterium Halomonas sp. SBC20 for its biocementation of U in CFA based on microbially induced calcite precipitation (MICP). Rectangle-shaped CFA bricks were made from CFA using bacterial cells, and an electric testing machine tested their compressive strength. U distribution pattern and immobility against rainfall runoff were carefully examined by a five-stage U sequential extraction method and a leaching column test. The microstructural changes in CFA bricks were characterized by FTIR and SEM-EDS methods. The results showed that the compressive strength of CFA bricks after being cultivated by bacterial cells increased considerably compared to control specimens. U mobility was significantly decreased in the exchangeable fraction, while the U content was markedly increased in the carbonate-bound fraction after biocementation. Much less U was released in the leaching column test after the treatment with bacterial cells. The FTIR and SEM-EDX methods confirmed the formation of carbonate precipitates and the incorporation of U into the calcite surfaces, obstructing the release of U into the surrounding environments. The technology provides an effective and economical treatment of U-contaminated CFA, which comes from coal mines with high uranium content in the Xinjiang region, even globally.

Novel Strategies for Concrete Restoration: a Deep Dive into Microbially Induced Calcite Precipitation Technology

Novel Strategies for Concrete Restoration: a Deep Dive into Microbially Induced Calcite Precipitation Technology

Numerical analysis of microbially induced calcite precipitation and enzyme induced calcite precipitation in calcareous sand: Multi-process and biochemical reactions

Microbially induced calcite precipitation (MICP) and Enzyme induced calcite precipitation (EICP) techniques were implemented to reinforce the large-scale calcareous sand in this study. Then a coupled numerical model to predict the biochemical reactions and hydraulic characteristics of MICP and EICP reaction was proposed and verified by physical experiments. Results showed that: This model could describe the variations of bacteria, calcium, calcite, permeability over time reasonably. It is necessary to consider the influence of the calculation domain scale when simulating the convection-diffusion-reaction in the multi-process of MICP and EICP reactions. The numerical and experimental values of calcite content are 0.841 g/cm3 and 0.861 g/cm3 for MICP-reinforced sand, 0.263 g/cm3 and 0.227 g/cm3 for EICP-reinforced sand after 192 h of reaction. The reaction rate krea is an important parameter to control the calcite content. Accordingly, the permeability coefficient of MICP and EICP reinforced calcareous sand decreases by 32% and 18%. Due to the influence of substance transportation and calcite precipitation, the calcite shows a trend of decreasing firstly and then increasing with the enhancing of the initial permeability coefficient in biochemical reactions. The optimal injecting ratio q11: q12 in this study is 100:300 mL/min. The process for the application of MICP and EICP coupled numerical model is also recommended, which provides reference for engineering projects in ground improvement.

Effects of bacterial strains on undrained cyclic behavior of bio-cemented sand considering wetting and drying cycles

The microbial-induced calcite precipitation (MICP) technique has been developed as a sustainable methodology for the improvement of the engineering characteristics of sandy soils. However, the efficiency of MICP-treated sand has not been well established in the literature considering cyclic loading under undrained conditions. Furthermore, the efficacy of different bacterial strains in enhancing the cyclic properties of MICP-treated sand has not been sufficiently documented. Moreover, the effect of wetting-drying (WD) cycles on the cyclic characteristics of MICP-treated sand is not readily available, which may contribute to the limited adoption of MICP treatment in field applications. In this study, strain-controlled consolidated undrained (CU) cyclic triaxial testing was conducted to evaluate the effects of MICP treatment on standard Ennore sand from India with two bacterial strains: Sporosarcina pasteurii and Bacillus subtilis. The treatment durations of 7 d and 14 d were considered, with an interval of 12 h between treatments. The cyclic characteristics, such as the shear modulus and damping ratio, of the MICP-treated sand with the different bacterial strains have been estimated and compared. Furthermore, the effect of WD cycles on the cyclic characteristics of MICP-treated sand has been evaluated considering 5–15 cycles and aging of samples up to three months. The findings of this study may be helpful in assessing the cyclic characteristics of MICP-treated sand, considering the influence of different bacterial strains, treatment duration, and WD cycles.

Potentiometric ion-selective membrane sensing of microbially induced calcite precipitation

Microbially induced calcite precipitation (MICP) is an emerging biogeotechnical technique that facilitates a bio-cementation process through biomineralisation and which can bond otherwise loose soil particles, thereby increasing the shear strength and stiffness of the soil while decreasing its permeability and compressibility. However, the uniformity of calcite (calcium carbonate) precipitation may have a relatively low ‘certainty of execution’ in situ due to strict environmental conditions required for the reaction, highlighting a strong need for in situ monitoring of the subsurface during and following the treatment. This research explored an electrochemical approach to monitoring MICP treatment through the development and use of potentiometric ion-selective sensors to measure ammonium and calcium ions continuously in both aqueous batch reactors and small laboratory-scale soil test cells. Sensor calibrations indicate a strong and reliable correlation between the electrochemical potential and target ion concentration, which shows promise in sensor technology for monitoring MICP and other similar processes. Improvements to the current design are, however, needed, as fouling due to calcite precipitation, and to some extent biofouling of the sensor, was observed in the highly dynamic, biomineralisation environment, which caused data drift/interference. These effects should be considered in data analysis and eliminated/minimised for eventual adoption towards monitoring the efficacy of MICP treatment in situ.