Microbial Colony Counts Research Articles

Effect of Stocking Density on Growth Performance, Proximate Composition and Hematology of Anabas testudineus in Biofloc Culture System

In a biofloc fish culture system, this experiment assessed the effects of stocking densities of 200, 250, and 300 fish/m3 at treatments T1, T2, and T3, respectively, on the growth and production performances of Anabas testudineus. With a mean final weight of 121.64 ± 1.354 g, T1 produced the largest weight, followed by T2 (111.63 ± 0.552 g) and T3 (104.65 ± 0.602 g). In addition, the feed conversion ratio in T1 treatment (0.97 ± 0.011) was noticeably lower than T2 (1.36 ± 0.020) and T3 treatment (1.67 ± 0.116). Survival rate in T1 treatment (86.66%) was substantially greater than T2 (80.71%) and T3 treatments (74.81%). The comparative analysis of proximate composition has shown lower total microbial colony count, higher protein and fat content, and lower moisture and ash content in biofloc system. There was no significant difference (p > 0.05) found in water quality parameter. Significant difference found (p < 0.05) in DO among three treatments. Comparing T1 (1.77 ± 0.017) to T2 (1.46 ± 0.005) and T3 (1.16 ± 0.047), BCR in T1 were higher. There was no significant differences found in HDL, LDL, Triglycerides and cortisol among three treatments but significantly found in control of A. testudineus. The higher level of HDL found in T1 and lower in control, LDL level lower in T1 and higher in control, triglycerides were lower in T1 and higher in control, cortisol higher in control and lower in T1. It might be concluded that the comparatively lower stocking density provides enhanced production and also be suitable in order to maintain better water quality parameters in biofloc culture system. This density is appropriate for the nation's biofloc culture of A. testudineus, as evidenced by the lowest production costs and maximum income seen in T1.

Evaluating annual soil carbon emissions under biochar-added farmland subjecting from freeze-thaw cycle

Straw biochar is a commonly recognized agricultural amendment that can improve soil quality and reduce carbon emissions while sequestering soil carbon. However, the mechanisms underlying biochar's effects on annual soil carbon emissions in seasonally frozen soil areas and intrinsic drivers have not been clarified. Here, a 2-y field experiment was conducted to investigate the effects of different biochar dosages (0, 15, and 30, t ha−1; B0 (CK), B15, and B30, respectively) on carbon emissions (CO2 and CH4) microbial colony count, and soil-environment factors. The study period was the full annual cycle, including the freeze-thaw period (FTP) and the crop growth period (CP). Structural equation modeling (SEM) was developed to reveal the key drivers and potential mechanisms of biochar on carbon emissions. Biochar application reduced soil carbon emissions, with the reduction rate positively related to the biochar application rate (B30 best). During FTP, the reduction rate was 11.5% for CO2 and 48.2% for CH4. During CP, the reduction rate was 17.9% for CO2 and 34.5% for CH4. Overall, compared with CK, B30 treatment had a significant effect on reducing total soil carbon emissions (P < 0.05), with an average decrease of 16.7% during the two-year test period. The study also showed that for soils with continuous annual cycles (FTP and CP), carbon emissions were best observed from 10:00–13:00. After two years of freeze-thaw cycling, biochar continued to improve soil physical and chemical properties, thereby increasing soil microbial colony count. Compared with B0, the B30 treatment significantly increased the total colony count by 74.3% and 263.8% during FTP and CP (P < 0.05). Structural equation modeling (SEM) indicated that, with or without biochar application, the soil physicochemical properties directly or indirectly affected soil CO2 and CH4 emission fluxes through microbial colony count. The total effects of biochar application on CO2 emission fluxes were 0.50 (P < 0.05) and 0.64 (P < 0.01), respectively, but there was no significant effect on CH4 emission fluxes (P > 0.05). Among them, soil water content (SWC), soil temperature (ST) and soil organic carbon (SOC) were the main environmental determinants of CO2 emission fluxes during the FTP and CP. The total effects were 0.57, 0.65, and 0.53, respectively. For CH4, SWC, soil salinity (SS) and actinomycete colony count were the main environmental factors affecting its emission. The total effects were 0.50, 0.45, 0.44, respectively. For freeze-thaw alternating soils, the application of biochar is a feasible option for addressing climate change through soil carbon sequestration and greenhouse gas emissions mitigation. Soil water-heat-salt-fertilization and microbial communities are important for soil carbon emissions as the reaction matrix and main participants of soil carbon and nitrogen biochemical transformation.

Tannic Acid Incorporated Antibacterial Polyethylene Glycol Based Hydrogel Sponges for Management of Wound Infections.

Conventional wound dressings fail to provide features that can assist the healing process of chronic wounds. Multifunctional wound dressings address this issue by incorporating attributes including antibacterial and antioxidant activity, and the ability to enhance wound healing. Herein, polyethylene glycol (PEG)-based antibacterial hydrogel sponge dressings are prepared by a rapid and facile gas foaming method based on an acid chloride/alcohol reaction where tannic acid (TA) is included as a reactant to impart antibacterial efficacy as well as to enhance the mechanical properties of the samples. The results reveal that the TA-integrated sponges possess excellent antibacterial properties against both Escherichia coli and Staphylococcus aureus with approximately 6-8 log reduction in the microbial colony count after 6h, indicating their high potential for management of infection-prone wounds. Compared to the control sample, TA incorporation increases the elastic modulus by twofold. As the samples also exhibit biocompatibility, antioxidant activity, and wound healing capacity, the novel TA-incorporated hydrogels can be an alternative to traditional wound dressings for wounds with low-to-moderate exudate.

Don't Rush with Your Brush: An In Vitro Study on Toothbrush Hygiene.

To evaluate the effectiveness of audiovisual (AV) aids in creating awareness of toothbrush hygiene. This randomized trial study was conducted on 40 children aged 6-11 years. Children were given soft toothbrushes and then collected after brushing for 14 days. The toothbrushes were collected and sent to the laboratory for microbial culture evaluation. A questionnaire consisting of various questions regarding daily toothbrush hygiene practices was filled by the parents at the same time. After 14 days, brushes were collected, and the parents were shown an AV aid regarding toothbrush decontamination, parental awareness, and supervision. Those children were given new sets of toothbrushes and instructed to decontaminate them with chlorhexidine solution. After 14 days, the same questionnaire was filled out by the parents and toothbrushes were collected and immediately sent to the laboratory for microbial culture evaluation. All the sampled toothbrushes had significant (p < 0.001) bacterial growth after 14 days of use. The use of disinfectant led to a 99.98% reduction in microbial colony counts. Hence, showed a significant result. Questionnaire analysis showed a positive parental approach toward maintaining toothbrush hygiene. Cleaning and disinfection of toothbrushes is crucial to stop the spread of disease since bacterial contamination cannot be 100% eliminated. AV aids can be effective tools for increasing awareness. Through this study, we want to emphasize toothbrush hygiene and create awareness for the same through AV aid because a healthy toothbrush leads to healthy oral health. Sharma S, Tandon S, Rai TS, et al. Don't Rush with Your Brush: An In Vitro Study on Toothbrush Hygiene. Int J Clin Pediatr Dent 2024;17(2):162-167.

Isolation of hitherto-uncultivated microorganisms- Application of radical scavengers.

The culture filtrates of the predominant bacterial strains isolated from soil samples have been shown to increase the microbial colony counts on agar plates used for the isolation of uncultured bacteria. One of the factors in the culture filtrates responsible for this increase was identified to be superoxide dismutase (SOD). The generation of reactive oxygen species (O2-, H2O2, and ・OH) was detected from conventional laboratory agar media. The use of agar media supplemented with radical scavengers (SOD, catalase, ascorbic acid, or rutin) effectively increased the colony counts and kinds of microbial strains that grew from soil samples. Taxonomical studies on these isolates revealed new taxa for phylum Actinomycetota; one family, three genera, and nine species were newly described. One of the strains, Patulibacter minatonensis KV-614T belonging to the new family Patulibacteraceae, was isolated on agar medium supplemented with SOD. P. minatonensis KV-614T represents a novel lineage within the phylum Actinomycetota. A polymerase chain reaction (PCR) study using specific primers for the detection of strains related to the genus Patulibacter, order Solirubrobacterales, showed a high distribution frequency, with detection in over 70% of the soil samples tested. These data suggest that the use of radical scavengers may facilitate the isolation of some hitherto-uncultivated microorganisms widely distributed in soil.

AN EXPERIMENTAL STUDY TO EVALUATE THE EFFICACY OF SPHATIKA IN THE PURIFICATION OF CONTAMINATED WATER W.S.R TO QUARTZ.

Water being the most essential and basic of life; it must be pure and free from any contaminants. Polluted water is main reason for waterborne diseases. Considering this, an experiment has conducted to study the efficacy of quartz in the purification of contaminated water which is simple and easy. Aims and Objectives: To carry out the experiment using Sphatika (Quartz) to evaluate its efficacy in the purification of contaminated water. Meth-odology: The water sample was taken in a glass bottle of 500ml; 1st group was used without any materials. Sec-ond group was subjected to filtration. Third group was added with Sphatika (quartz) in the quantity of 10, 25 and 50 grams. Each of the samples was assessed after 6, 12 and 24 hrs: for the physical, chemical and microbial pa-rameters. Results: Quartz reduces Salinity, Total Hardness (TH) and Magnesium; and increases pH, Conductivi-ty, Total Dissolved Solids (TDS), Dissolved Oxygen (DO), Total Alkalinity (TA), Carbonate and Bicarbonate. While there was no change in Calcium. After adding of quartz, the microbial colony count further increased. Conclusion: Quartz showed positive results in some of the parameters. It doesn’t possess an antibacterial effect. It can be used as a medium in filters.

Microbiological Evaluation of Four Different Suture Materials Used for the Surgical Removal of Impacted Lower Third Molars: A Single-Center Prospective Comparative Study.

Introduction Sutures play a crucial role in the postoperative healing process, as they help approximate wound edges, promote hemostasis, and support tissue healing. The oral cavity harbors a diverse microbial population, and oral surgical procedures can introduce potential pathogens into the surgical site. Understanding the impact of suture material on wound infection rates and the colonization of potentially harmful microorganisms is vital for improving patient outcomes. This study was aimed to evaluate and compare the microbiological properties of prolene, vicryl plus, monocryl, and silk sutures used after the surgical removal of impacted lower third molars. Materials and methods A total of 40 patients requiring surgical extraction of impacted lower third molars wereassigned to four groups: prolene, vicryl plus, monocryl, and silk sutures. Surgical extraction of impacted toothwas done, and wound was sutured with the abovementioned four different materials in four groups, respectively. After seven days, the sutures were removedand sent to the microbiology lab for colony count assessment. Total microbial colony count, streptococcus count, and lactobacillus count were assessed.Data was analyzed using IBM SPSS Statistics for Windows, Version 23.0 (Released 2015; IBM Corp., Armonk, New York, United States) with p-values less than 0.05 considered as statistically significant. The one-way analysis of variance (ANOVA) and post-hoc Tukey test were done to compare intergroup relations. Results The microbiological evaluation of the sutures revealed significant differences in bacterial colonization among the four groups. More bacterial quantitieswere found in the silk group followed by the monocryl, vicryl plus, and prolene groups in the descending order. Prolene demonstrated the lowest incidence of bacterial growth (p<0.001) compared to vicryl plus, monocryl, and silk sutures.Bacterial colony count was highest in the silk group. The predominant bacterial species found in all groups were Streptococcus viridans, Staphylococcus aureus, and Lactobacillus. Conclusion It was found that prolene and vicryl plus sutures exhibited superior microbiological properties compared to monocryl and silk sutures when used for the surgical removal of impacted lower third molars. The lower incidence and less quantityof bacterial colonization on prolene sutures suggest their potential for reducing the risk of postoperative infection; hence, these sutures can be preferred for oral surgical procedures.

Evaluation of Physical Properties of Denture Base Resins Containing Silver Nanoparticles of Aloe barbadensis Miller, Morinda citrifolia, and Boesenbergia rotunda and Its Anti-microbial Effect: An In Vitro Study.

Introduction The denture bases fabricated from polymethylmethacrylate (PMMA) have some disadvantages, such as surface prone to microbial growth and biofilm accumulation, which contributes to the onset and dissemination of infections among denture wearers. Therefore, the purpose of this in vitro study was to evaluate the flexural strength, hardness, and antimicrobial effect of denture base resin incorporated with 0.05% and 0.1% silver nanoparticles (AgNPs) of Aloe barbadensis miller (aloe vera), Morinda citrifolia (noni), and Boesenbergia rotunda (finger root). Materials and methods A total of 84 PMMAsamples were used and were divided into three groups. Flexural strength tests were performed on Group 1 PMMAblocks. Group 2 involved hardness testing of PMMAblocks, whereas Group 3 involved antimicrobial activity. Each group was subsequently split into seven subgroups with differing concentrations of AgNPs: Sub Group 1: control (no AgNPs),Sub Group 2: 0.05% aloe vera AgNPs, Sub Group 3: 0.1% aloe vera AgNPs, Sub Group 4: 0.05% noni AgNPs, Sub Group 5: 0.1% of noni AgNPs,Sub Group 6: 0.05%finger root AgNPs, and Sub Group 7: 0.1% finger root AgNPs.The flexural strength was evaluated usinga universal testing machine (Instron 8801). Surface hardness was measured using a Vickers tester (Tukon 1102). For the antimicrobial activity analysis, the samples were incubated in a suitable culture broth containing Candida albicans for 24 hours. Microbial colony count (colony-forming unit (CFU)/mL) was estimated to evaluate the microbial adhesion to the surface of the denture base materials. Statistical analysis The flexural strength, hardness, and CFU between the groups were analyzed using one-way analysis of variance (ANOVA) followed by multiple comparisons with Tukey's honest significant difference (HSD) test (α=0.05). The level of statistical significance was determined at p<0.05. Results It was observed that the mean flexural strength was maximum in PMMA incorporated with 0.05% of aloe vera AgNPs and least in PMMA incorporated with 0.1% noni AgNPs. It was seen that a steady loss in flexural strength is observed from 0.05% to 0.1%. The mean hardness was maximum in PMMA incorporated with 0.1% of noni AgNPs and least in PMMA incorporated with 0.05% aloe vera AgNPs. It was also found that the hardness was directly proportional to the number of nanoparticles. With an increase in the weight percentage of nanoparticles, a steady increase in hardness was seen in all the test groups. In our study, the results showed that finger root 0.1% showed the least CFU with a significant reduction of C. albicans adherence; therefore, it indicates higher anti-fungal activity. Aloe vera 0.05% showed the lowest inhibition of C. albicans,suggesting the least anti-fungal activity. Conclusion Within the limitations of this study, It can thus be concluded that the addition of AgNPs incorporated with plant extracts of Aloe barbadensis miller (aloe vera), Morinda citrifolia (noni), and Boesenbergia rotunda (finger root) can alter the flexural strength, hardness, and microbial adhesion of PMMA. In our study, it can be concluded that flexural strength increases with the addition of AgNPs of 0.5% concentration after which a steady loss is seen. However, the hardness and antimicrobial activity increased with an increase in the concentration of AgNPs in all three plant extracts.

Application of compost assisted by Fe3O4 nanoparticles in di (2-ethylhexyl) phthalate-contaminated soil remediation: Biostimulation strategy, Soil responses, and RSM/CCD Optimization

Globally, contamination of agricultural soils by phthalate esters (PAEs) caused by direct consumption of plastic mulch films has been confirmed. The most widely used plasticizer is di (2-ethylhexyl) phthalate (DEHP), which is a more recalcitrant endocrine-disrupting chemical (EDC). Because of its low solubility and hydrophobicity, it remains in the soil longer, causes bioaccumulation in agricultural products, and has negative repercussions for food safety. In this study, the performance of kitchen organic waste compost assisted by Fe3O4 nanoparticles in DEHP removal efficiency (%) and soil C:N ratio (two responses) was optimized using Response Surface Methodology (RSM) based on Central Composite Design (CCD) in Design-Expert software (11.0.3.0). Under optimum conditions, a DEHP concentration of 10 mg·kg−1 (dw soil), a retention time of 35 days, an NPs dose of 0.99 g·kg−1 (media), a removal efficiency of 91.6 %, and a soil C:N ratio of 10.5 with a desirability of 0.963 were determined. A quadratic model (P-value <0.0001, adjusted R2 = 0.974 (Y1), 0.943 (Y2)) was used to predict the variables and their interactions. The agricultural soil responses in the treatments amended by compost and Fe3O4 NPs (SCN) showed a significant increase in SOM, TC, TN, AP, K, and Fe nutrients when compared to the control (P < 0.05). After 35 days, in the SC1N3 treatment (DEHP concentration = 10 mg·kg−1, NPs dose =1.2 g·kg−1), with higher DEHP removal efficiency (89.57 %), the C:N:P ratio was equal to 100: 9.75:0.69, and the total microbial colony count was 3.6 × 109 CFU/ml at pH 7.45. The study found that compost nutrients and Fe-based nanoparticle micronutrients can enhance DEHP degradation by stimulating the soil's native microflora. As a result, the synergistic potential of compost and Fe3O4 nanoparticles can be considered a promising, cost-effective, and agri-environmentally friendly approach in the “assisted bioremediation” strategy of DEHP-contaminated soils.

The Validity of the Poisson Distribution to Analyze Microbial Colony Counts on Agar Plates for Food Samples

Microbial colony counts of food samples in microbiological examinations are one of the most important items. The probability distributions for the colony counts per agar plate at the dilution of counting had not been intensively studied so far. Recently we analyzed the colony counts of food samples with several probability distributions using the Pearson's chi-square value by the "traditional" statistics as the index of fit [Fujikawa and Tsubaki, Food Hyg.Saf.Sc., 60, 88-95 (2019)]. As a result, the selected probability distributions depended on the samples. In this study we newly selected a probability distribution, namely a statistical model, suitable for the above data with the method of maximum likelihood from the probabilistic point of view. The Akaike's Information Criterion (AIC) was used as the index of fit. Consequently, the Poisson model were better than the negative binomial model for all of four food samples. The Poisson model was also better than the binomial for three of four microbial culture samples. With Baysian Information Criterion (BIC), the Poisson model was also better than these two models for all the samples. These results suggested that the Poisson distribution would be the best model to estimate the colony counts of food samples. The present study would be the first report on the statistical model selection for the colony counts of food samples with AIC and BIC.

EVALUATING CHANGES IN SALIVARY FLOW RATE, PH &amp; MICROBIAL COUNT DURING FIXED ORTHODONTIC TREATMENT - AN IN VIVO STUDY

Aim of the study: Aim of this study is to assess the changes in salivary flow rate, salivary pH, plaque index, Streptococcus mutans, Lactobacillus, and Candida albicans in patients undergoing fixed orthodontic treatment, before and after placement of fixed orthodontic appliances. Materials and Method: In present study salivary samples from 60 patients (30 Males and 30 Females) with age group of 14-25 years would be undergoing fixed orthodontic treatment at the department of orthodontics. Salivary flow rate, pH, plaque index and microbial colony count were measured. Streptococcus mutans, Lactobacillus and Candida albicans colony counts were measured using mitis salivarius agar, rogosa agar and sabouraud dextrose agar respectively. Saliva collected for 5 mins and measured in ml/min. salivary pH measured with digital pH meter. Salivary samples obtained from the same patients at intervals of 8 weeks and 16 weeks after commencement of orthodontic treatment and evaluated for the same. Result: This study showed fixed orthodontic appliances increases salivary flow rate (p=0.0001), decreases salivary pH (p=0.03), increases plaque index (p=0.0001), also increases Streptococcus mutans, Lactobacillus and Candida albicans colony count (p=0.0003, p=0.0001and p=0.019 respectively). Independent t test reported salivary flow rate significantly increases more in male patients at 16 weeks interval (p=0.03). Conclusion: The results of this study confirmed that after placement of fixed orthodontic appliances plaque retention increases, which sequentially decrease the salivary pH and decreased salivary pH promote the growth of microbiota.

Effect of Chlorhexidine and Tea Tree Oil on Reducing the Number of Oral Microorganisms.

Thus, the aim of this study was to compare the effect of using two preoperative mouthwashes (0.12% chlorhexidine and 0.2% tea tree oil) on the number of colonies of oral microorganisms. Forty participants who needed to be rehabilitated with dental implants were included in this study. They were randomly divided into two groups (chlorhexidine group and tea tree group; n = 20, each). For each group, saliva samples were collected at four different times: T0 (initially)-before using the mouthwash, T1-after 1 minute of using the mouthwash, T10-after 10 minutes of using the mouthwash, and T60-after 60 minutes of using the mouthwash. At T0 and T1, saliva samples were collected before implant placement surgery, and at T10 and T60, saliva samples were collected during surgery. In each group, one saliva sample was collected at each evaluated time point for each patient, totaling 4 saliva collections per patient. MSB agar (Mitis-Salivarius-Bacitracin) and BHI agar (Brain Heart Infusion) culture media were used in each group. Microbial colony counts were performed using a magnifying glass and recorded in CFU (colony forming units)/mL. Statistical analyses were performed using the Friedman, Mann-Whitney U and Wilcoxon tests (p < 0.05). Based on MSB agar culture medium, at T0, the number of Streptococcus mutans colonies in the chlorhexidine group was significantly higher compared with the tea tree group (p <0.05; MSB agar). The chlorhexidine group showed significantly lower CFU/mL values for Streptococcus mutans at T1, T10, and T60 compared with the tea tree group (p <0.05; MSB agar). Based on BHI agar culture medium, at T0, the chlorhexidine group showed a significantly lower value of CFU/mL compared with the tea tree group (p < 0.05; BHI agar). At T1, T10, and T60, the chlorhexidine group showed significantly lower CFU/mL values compared with the tea tree group (p <0.05; BHI agar). Chlorhexidine is more indicated as a preoperative mouthwash than tea tree oil, due to its significantly more effective antimicrobial action.

Preparation and evaluation of a novel chlorine dioxide preservative based on citric acid grafted carboxymethyl chitosan

Instrument-free chlorine dioxide (ClO2) preservative for fruit and vegetable has gained great attention due to its convenience and safety. In this study, a series of carboxymethyl chitosan (CMC) with citric acid (CA) substituents were synthesized, characterized, and further used to prepare a novel ClO2 slow-releasing preservative for longan. UV–Vis and FT-IR spectra revealed that CMC-CA#1–3 were successfully prepared. Further potentiometric titration showed that the mass ratios of CA grafted in CMC-CA#1–3 were 0.18:1, 0.42:1, and 0.42:1, respectively. The composition and concentration of ClO2 slow-releasing preservative were optimized, and the best formulation was as follows: NaClO2:CMC-CA#2:Na2SO4:starch = 3:2:1:1. The maximum ClO2 release time of this preservative reached >240 h at 5–25 °C, and the maximum release rate always occurred at 12–36 h. Longan treated with 0.15–1.2 g ClO2 preservative had significantly (p < 0.05) higher L* and a* values but lower respiration rate and total microbial colony counts than the CK group (0 g ClO2 preservative). After 17 days of storage, longan treated with 0.3 g ClO2 preservative had the highest L* value of 47.47 and lowest respiration rate of 34.42 mg·kg−1·h−1, showing the best pericarp color and pulp quality. This study provided a safe, effective, and simple solution for longan preservation.

Adipose-derived Stem Cells Improving Inflammatory and Proliferative Responses in an Infected and Ischemic Ulcer Model in Type 1 Diabetic Rats: Insights Into Bacterial Count, Stereological Parameters, microRNA-21, and FGF2 Regulation

Background: Adipose-derived stem cells (ADSCs) have been shown to enhance wound healing in rats with type 1 diabetes (DM1). Objectives: This experimental study aimed to explore how ADSC administration affects bacterial count, wound size, biomechanical and stereological parameters, and the expression of microRNA-21 and FGF2 in a rat model of infected, ischemic, and delayed wound healing in DM1. Methods: Twenty-four male adult Wistar rats weighing less than 250 g were randomly assigned to four groups (n = 6 per group). Type 1 diabetes was induced in all animals, resulting in the development of a delayed, ischemic, and infected wound model. The CGday4 and CGday8 groups served as controls. In the AGday4 group, the animals received allograft h-ADSs and were euthanized on day four after surgery. Similarly, in the AGday8 group, the animals received h-ADSs and were euthanized on day eight after surgery. Microbial colony counts, wound size, stereological parameters, and the expression of microRNA-21 and FGF2 were evaluated in this study during the inflammation (day 4) and proliferation (day 8) stages of wound healing. Results: We demonstrated that h-ADSs significantly reduced microbiological counts compared to the control group on days 4 and 8. Moreover, in the AGday8 group compared to the AGday4 group, this decline in microbiological counts was even more pronounced. Moreover, we observed that the stereological characteristics in the AGday4 and AGday8 groups were significantly superior to those in the CG groups. Additionally, the AGday4 and AGday8 groups exhibited smaller ulcer area sizes compared to the CG groups. Furthermore, the AGday4 and AGday8 groups demonstrated higher expression levels of FGF2 and microRNA-21 than the CG groups on days 4 and 8. Notably, on day 8, the AGday8 group’s outcomes surpassed those of the AGday4 group (P &lt; 0.01). Conclusions: Through lowering microbial counts, modifying stereological parameters, microRNA-21, and FGF2 expression, the administration of hADS dramatically speeds up the healing of MARS-infected and ischemic ulcers in DM1 rats.

Dipping Titan implants in Irrisept solution (0.05% chlorhexidine gluconate) and exposure to various aerobic, anaerobic, and fungal species.

The organisms causing penile implant infections are changing from predominantly indolent gram-positive infections to more aggressive gram-negative and fungal infections because of antibiotic selection pressures based on novel next-generation sequencing DNA data. To evaluate the effectiveness of Irrisept solution (0.05% chlorhexidine gluconate) in decreasing isolate colony counts from a Titan implant by using a novel kill time washout methodology to mirror real-world usage. Sterilized Titan discs were dipped in Irrisept or saline. An inoculum of 109 organisms of a single bacterial or fungal species was placed on the discs. Bacterial and fungal strains were tested: Bacteroides fragilis, Candida albicans, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Staphylococcus epidermidis. The discs were then irrigated 3 times with Irrisept or saline. Microorganisms were sonicated off the discs and placed on appropriate agar and conditions for each species. The plates were incubated for 48 to 72hours at the temperature and under the conditions appropriate for each species. Colonies on the plates were hand counted. Irrisept effectively decreased microbial colony counts in all the species tested. Irrisept was shown to effectively decrease microbial colony counts from 3 to 6 log10 in all species tested. A 3-log10 reduction is considered the target level of performance that would indicate that a compound or product has effective killing activity against an organism of interest. The saline control with bulb syringe irrigation did not demonstrate reduction of microbial colony counts in any of the species tested. Irrisept is effective against all of the organisms causing modern-day infections with penile implant surgery and may decrease clinical infection rates to lower levels. The strength of this study is that we used quantitative microbial reduction counting and the largest array of bacterial and fungal species causing modern-day penile implant infections. The limitation is that this is an in vitro study and the clinical implications of our findings are not yet known. Quantitative microbial reduction counting shows that Irrisept is effective against the most commonly known modern-day organisms causing penile implant infections.

MP62-04 DIPPING TITAN ® IMPLANTS IN IRRISEPT ® (0.05% CHLORHEXIDINE GLUCONATE) SOLUTION AND EXPOSURE TO VARIOUS AEROBIC, ANAEROBIC AND FUNGAL SPECIES: IN VITRO STUDY RESULTS FROM A NOVEL KILL-TIME-WASHOUT METHODOLOGY TO EVALUATE REAL WORLD SITUATIONS

You have accessJournal of UrologyCME1 Apr 2023MP62-04 DIPPING TITAN® IMPLANTS IN IRRISEPT® (0.05% CHLORHEXIDINE GLUCONATE) SOLUTION AND EXPOSURE TO VARIOUS AEROBIC, ANAEROBIC AND FUNGAL SPECIES: IN VITRO STUDY RESULTS FROM A NOVEL KILL-TIME-WASHOUT METHODOLOGY TO EVALUATE REAL WORLD SITUATIONS Edward Karpman, Griggs Ryan, Carolyn twomey, and Gerard Henry Edward KarpmanEdward Karpman More articles by this author , Griggs RyanGriggs Ryan More articles by this author , Carolyn twomey Carolyn twomey More articles by this author , and Gerard HenryGerard Henry More articles by this author View All Author Informationhttps://doi.org/10.1097/JU.0000000000003320.04AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The organisms causing penile implant infections are changing from predominantly indolent gram-positive infections to more aggressive gram-negative and fungal infections because of antibiotic selection pressures and novel next generation sequencing DNA data. Our objective is to evaluate the effectiveness of Irrisept® (0.05% chlorhexidine solution) to decrease isolate colony counts off of a Titan implant using a novel kill time washout methodology to mirror real-world usage. METHODS: Sterilized Titan discs were dipped in Irrisept® or saline. An inoculum of 109 organisms was placed on the discs. Bacterial and fungal strains tested were Bacteroides fragilis ATCC #25285, Candida albicans ATCC #10231, Enterococcus faecalis ATCC #29212, Escherichia coli #25922, Pseudomonas aeruginosa ATCC #27853, Staphylococcus aureus ATCC# 29213, and Staphylococcus epidermidis ATCC # 12228. The discs were then irrigated 3 times with Irrisept or saline. Microorganisms were sonicated off of the discs and placed on appropriate agar and conditions for each species. The plates were incubated at the temperature and under the conditions appropriate for each species for 48 to 72 hours. Colonies on the plates were hand counted. RESULTS: Irrisept® was shown to effectively decrease microbial colony counts from 3-6 log10 in all species tested. 3 log10 reductions are considered the significant level of performance that would indicate a compound or product has substantial killing activity against an organism of interest. The saline control with bulb syringe irrigation did not demonstrate reduction of microbial colony counts in any of the species tested. CONCLUSIONS: Irrisept® is effective against all of the organisms causing modern day infections with penile implant surgery and may decrease clinical infection rates to lower levels. The strength of this study is that we used microbial reduction counts with a real-world kill-time-washout methodology that is superior to zone of inhibition testing, which is the basis of all previous studies on this subject matter. The limitation is that this is an in vitro study and the clinical implications of our findings are not yet known. Source of Funding: Irrimax Corporation, Coloplast Corporation © 2023 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 209Issue Supplement 4April 2023Page: e862 Advertisement Copyright & Permissions© 2023 by American Urological Association Education and Research, Inc.MetricsAuthor Information Edward Karpman More articles by this author Griggs Ryan More articles by this author Carolyn twomey More articles by this author Gerard Henry More articles by this author Expand All Advertisement PDF downloadLoading ...

Substituting Natural Honey for Cane Sugar (Sucrose) Retards Microbial Growth Independent of Water Activity During Storage of Tomato Jam

Today, there are several attempts to make nutritious food products low in glycemic carbohydrates as this can partly help in solving the ever-growing numbers of type 2 diabetes and obesity. We thus made two types of jam, one using conventional sucrose with a high glycemic index and the other using honey which is regarded as a low glycemic index carbohydrate. Honey jam had comparable physical properties with sucrose jam. The total soluble solid (Brix) was significantly lower in honey jam than in sucrose jam (p=0.04) indicating less sugar in honey jam. Both jams had comparatively higher percent moisture content than other jams reported in literature potentially suggesting reduced storage stability. Despite having higher water activity, honey jam had progressive decrease in microbial colony counts suggesting honey was inhibitory to growth of microorganism compared to sucrose jam. This effect was independent of water activity as sucrose jam which had lower water activity promoted growth of microorganism during storage. Therefore, substituting natural honey for sucrose during formulation of tomato jam retards microbial growth thereby prolonging shelf life.

Comparative Evaluation of Effects of Kavala Gandusha with Rice Bran Oil and Mustard Oil on Unstimulated Salivary pH, Dental Plaque and Gingivitis - A Clinico–Biochemical Double Blind Study

Introduction Gingivitis is an inflammatory condition caused by bacterial biofilm that accumulates on teeth. It is a complex disease that involves the loss of attachment around teeth due to the action of microorganisms which is a major cause of tooth loss in adults. Tooth brushing is the most accepted practice for the maintenance of oral hygiene. Chemotherapeutic agents such as mouthwashes containing chlorhexidine have been suggested as an adjuvant in reducing plaque formation however they can be associated with certain undesirable adverse effects. Keeping that in mind a novel traditional Indian method called oil pulling is regaining popularity. Oil pulling involves swishing oil in the mouth for oral and systemic health benefits.Aim The present study focused on benefits of oil pulling in maintaining daily oral hygiene and its effects on oral microfloral colony counts.Methodology The study period was six months and the duration of the study was 21 days. Evaluation of salivary pH dental plaque and gingivitis was done at baseline 0 after 7 14 and 21 days. After recording baseline data patients underwent thorough scaling and root planing. Patients were divided randomly into Group 1 and 2 and bottles containing mustard oil and rice bran oil to swish were distributed respectively. Instruction to swish 10 ml of the given oil twice daily for about 1 to 2 minutes was given.Results Reduction in the colony count was greater with rice bran oil than mustard oil.Conclusion Rice bran oil and mustard oil were found to be effective in the maintenance of oral health. Rice bran oil was found to be more effective compared to mustard oil in reducing the oral microbial colony count and plaque.

Effectiveness of combination of heat water disinfection, continuous water circulation, and minimalized dead space for dialysis piping in maintaining ultrapure dialysis fluid and preventing biofilm formation in a central dialysis fluid delivery system.

Various benefits have been attached to purifying the dialysis fluid used for hemodialysis therapy. The central dialysis fluid delivery system can treat approximately 50 dialysis patients simultaneously and is convenient to operate. In contrast, the dialysis fluid supply piping is complicated, and bacterial growth can cause biofilms. This study aimed to develop sustainable cleaning strategies to solve the complicated dialysis fluid piping, which is a weakness of the central dialysis fluid delivery system, and provide ultrapure dialysis fluid for a long term. Combination of heat water disinfection, continuous water circulation, and minimalized dead space in the dialysis piping were designed for a central dialysis fluid delivery system and used in a clinic for 6years. As an index of water purification, endotoxin concentrations and microbial colony counts in reverse osmosis water and dialysis fluid were measured. In addition, we performed scanning electron microscopy of the silicon tube surface that had been used for 5years to confirm the presence or absence of biofilm formation. For 6years, endotoxin concentrations and microbial colonies were not detected in reverse osmosis water and dialysis fluid using the multiple-patient dialysis fluid supply equipment. The purity of the dialysis fluid was maintained. No biofilm formation was observed by scanning electron microscopy. Combination of heat water disinfection, continuous water circulation, and minimalized dead space designs for dialysis piping can supply ultrapure dialysis fluid with minimal biofilm formation in the piping in the long term.

The Level of Contamination of Fura Sold in Karu Local Government Area of Nasarawa State of Nigeria

Locally prepared Fura da Nono was obtained from three different markets each of Masaka, Auta and Nyanya districts all in Karu Local Government Area of Nasarawa State, Nigeria. Bacteriological analysis were conducted on the samples for isolation and identification of pathogenic and spoilage bacteria. Viable colony counts were determined and the result reveals that the colony count ranged from 4.0 x 104 to 7.0 x 104 cfu/ml. The bacteria isolated were Lactobacillus, Klebsiella, Micrococcus, and Salmonella species also, Pseudomonas aeruginosa, Serratia mercescens, Bacillus pumillus, and Staphylococcus aureus, with Lactobacillus species, Micrococcus species and Staphylococcus aureus having the highest levels of occurrence, having been isolated from all the samples analyzed. The high microbial colony counts and the presence of the above isolated bacteria may very well be related with free prevalence of some diseases experienced by some people consuming locally prepared Fura da Nono, especially students of Bingham University Karu, community. Since ‘Fura da Nono’ serve as one of the major source of beverages for the inhabitants of Karu Local Government Area. It is recommended that bacteriological examination of the food be carried out periodically so as to assess their suitability for consumption also regulatory agencies should carry out surveillance on hawkers of the food and public enlightenment campaign should be embarked upon. . Based on the result with the use of use of spearman’s rank correlation there is high correlation between the low percentage of the isolated bacteria from the control fura samples and the high percentage of bacteria detected from the test fura. (R= 0.8) Based on the result the level of bacterial load gotten from the Masaka, Auta, and Nyanya is higher than that of the control (Habib fura).