Microalgae Cultivation Systems Research Articles

Investigating the impacts of a recirculation sedimentation application on microalgae biomass cultivation in wastewater treatment

Commercial microalgae production is often interrupted by contamination, leading to short production cycles, reinoculation needs, and culture collapses, significantly increasing costs. This study focuses on investigating Recirculated Sedimentation Application (RSA) to control contamination in microalgae culture systems used for wastewater treatment. Chlorella vulgaris culture was grown in an unsterilized mixture of tertiary treatment effluent and centrate of anaerobic digestion wastewater sludge over a 90-day experimental period. 60 L raceway reactor was operated under a light intensity of 275 μM m−2.s−1 with a 16:8 h light-dark photoperiod. To evaluate the effect of RSA on biological-based problems, the experiment was conducted in three phases. The benefits of utilizing RSA were established through the following observations: effective removal of contaminants at an acceptable level without releasing the culture; extension of the biofilm formation time on the inner walls; inhibition of heterotrophic bacteria and nitrification; enhancement of the suspended solids retention capacity of the raceway tank (up to 770 mg.L−1); and improvement in ammonium removal rate to approximately 30 mg.L−1d−1. The ideal salinity level for both ammonium removal and biomass concentration in RSA should be below 0.02%. These findings demonstrate the potential of phycoremediation for sustainable wastewater treatment and contribute to environmental bioremediation strategies.

Light footprint of microalgae cultivation system addressed by modified Cornet model

The cultivation of microalgae is significantly influenced by light intensity and utilization efficiency. This study developed a modified Cornet (M−Cornet) model to assess the distribution of light intensity and flux in microalgae cultivation systems. Algal biofilm cultivation represents a more concentrated approach of algal suspension cultivation. Both follow the M−Cornet model and exhibit the same growth rates when cultured under identical conditions. Algal pigments and morphologies greatly impact the light absorption and scattering, resulting in light attenuation in intensity, penetration distance and light flux distribution. Algae varieties exhibit diverse light flux characteristics. 37% − 90% of the incident light is absorbed, of which, 80% to 90% is dissipated as heat. 10% to 63% of the incident light is scattered off the photobioreactor. The overall light utilization efficiency ranges 6% to 13%. The light footprint using the M−Cornet model offers valuable insights for photobioreactors designing and cultivation operating.

Sequential two-stage cultivation system using novel microalga consortia for treatment of municipal wastewater and simultaneous biomass production: Sustainable environmental management

Monoculture-based microalgae cultivation systems to treat wastewater are well-reported. Despite that, this method has some limitations in terms of nutrient removal potential, environment adaptation, and low biomass productivity. Conversely, microalgae co-cultivation and a two-stage sequential cultivation system (TSSCS) recently emerged as a promising approach to improve the treatment process and biomass productivity through better adaptation to the environment. However, no outdoor large-scale experiments were reported using this approach which hinders the viability of the process. Thus, in the present study, a sequential two-stage large-scale outdoor novel microalgae consortia experiment was developed. In first stage consortia-assisted sequential cultivation, two ratios of Tetraselmis indica (TS) and one ratio of Picochlorum sp. (PC) (2 TS:1 PC) were cultivated in a 1000-L pond containing 75%-municipal wastewater (MWW) + 25%-ASN-III, while in the second stage, 2 PC:1 TS was cultivated in two different ponds, and each containing 375-L 2 TS:1 PC-treated water + 375-L ASN-III. Outdoor parameters and nutrient removal efficiency (NRE), biomass, and biomolecule productivity such as lipid, photosynthetic pigments, astaxanthin, and β-carotene were quantified, and cost analysis was performed. At the end of the first and second stages, 2 TS:1 PC and 2 PC:1 TS showed maximum NRE of COD (68.71 and 86.40%), TN (66.98 and 94.73%), and TP (82.70 and 94.36%), respectively. Moreover, 2 TS:1 PC and 2 PC:1 TS Pond 1 and 2 produced maximum dry biomass production; 2.41 and ∼2.54 g/L contained lipid content; 36.89 and 34.90% that have 86.50 and 55.79% FAME content respectively. Similarly, 2 TS:1 PC and 2 PC:1 TS biomass exhibited valuable pigments production of astaxanthin i.e., 0.56 and 0.35 mg/g, and β-carotene; 4.65 and 2.82 mg/g, respectively. The cost analysis suggested that only microalgal-based MWW treatment was unfeasible, while valorization of produced biomass into co-products could offset the operation costs and could allow the option for the microalgal-based sustainable approach for the treatment of MWW and recovery of valuable resources.

Application of hybrid multi-criteria decision-making approach to analyze wastewater microalgae culture systems for bioenergy production

Bioenergy generation from microalgae can significantly contribute to climate mitigation and renewable energy production. In this regard, several multi-criteria decision-making method were employed to prioritize appropriate microalgae culture system for bioenergy production. Entropy weight, Criteria Importance Through Intercriteria Correlation (CRITIC) and Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) were the employed MCDA method. Fourteen microalgae culture systems were selected as a case study, which contain teen monoculture and four dual-culture. Initially, through ans in-depth review of the literature and expert views, four categories total eight indicators were selected as the evaluation indices of the study, namely 1) Proliferation: Half growth cycle and Max growth rate,2) Biomass output: Bio-crude yield and Lipid yield, 3) Nutrient utilization: residual concentration of total Nitrogen and total Phosphorus, and, 4) Stability: coefficient of variation of Bio-crude yield and Lipid yield. The result indicated that "Pediastrum sp. &Micractinium sp." was identified as the most bioenergy potential microalgae culture system, and the evaluation results of entropy weight method and CRITIC method are similar. It is pertinent to note that 1)the entropy weight method exhibits lower sample size requirements, 2) the critic method excels when dealing with larger sample sizes, and 3) the TOPSIS method necessitates the incorporation of appropriate weighting methods to ensure credible results. In the application stage, the key indicators related to cost can be further included in the evaluation indices.

A Comparative Analysis Assessing Growth Dynamics of Locally Isolated Chlorella sorokiniana and Chlorella vulgaris for Biomass and Lipid Production with Biodiesel Potential

Prior research has emphasized the potential of microalgae in biodiesel production, driven by their ability to replace fossil fuels. However, the significant costs associated with microalgae cultivation present a major obstacle to scaling up production. This study aims to develop an eco-friendly microalgae cultivation system by integrating carbon dioxide from flue gas emissions with an affordable photobioreactor, providing a sustainable biomass production. The research evaluates the growth performance of Chlorella sorokiniana and Chlorella vulgaris across this integrated system for biomass and lipid production. Results indicate substantial biomass yields of 1.97 and 1.84 g/L, with lipid contents of 35 % and 41 % for C. sorokiniana and C. vulgaris, respectively. The macrobubble photobioreactor demonstrates high potential for microalgae biomass and lipid production, yielding quality fatty acid methyl esters such as palmitic, linoleic and stearic. This study presents an environmentally friendly system for efficient microalgae cultivation, generating lipid-rich biomass suitable for biodiesel production.

Recent advancements in harnessing biodiesel from microalgae through attached growth systems

Conventional microalgal cultivation method relied on suspended growth or attached growth that uses inert solid surfaces, requiring the addition of external carbon sources to promote microalgal growth. The inert solid substrates for attached microalgae pose constraints on the maximal capabilities of these microorganisms as it does not possess the ability to provide the necessary nutritional support required for optimal microalgal growth. As a result, microalgae are obliged to only rely on the solid substrate as a means of attachment, without any further nutritional provisions. Slower growth rates, larger resource requirements, and exorbitant operating expenses had prompted the exploration of an alternative strategy, i.e; the innovative use of solid organic waste as nutritional source cum attachment platform. The findings of this review unveil key insights into microalgae cultivation system, offering a solution to economic challenges in the production phase. This innovation not only promotes economic feasibility and sustainability, but also contributes to economically viable and environmentally sustainable biofuel production.

Thermal comfort, daylight, and energy performance of envelope-integrated algae-based bioshading and static shading systems through multi-objective optimization

Energy-efficient envelopes integrated with different shading devices, as noticeable passive design strategies, have been of great interest for research studies. However, there is a lack of attention on optimizing envelope-integrated novel algae-based bioshading systems (ABBS) in comparison with other shading devices to enhance buildings’ sustainability-oriented performances. Integrating microalgae culture system with building façade as a state-of-the-art bioshading system is among recent developments in high-performance architecture. Although, challenges/limitations concerning different performances of this technology have not been extensively addressed. The present study conducts a multi-objective optimization framework to investigate the Thermal Comfort Percentage (TCP), Useful Daylight Illuminance (UDI), and Energy Usage Intensity (EUI) in a school building to evaluate the relationship between the performance objectives and design variables (shading characteristics, window-to-wall ratio (WWR)) through comparing different static shading systems and ABBS in the hot (BSh) and cold semi-arid (BSk) climates. The Technique for Order of Preference by Similarity to Ideal Solution (TOPSIS) method was also applied to determine the best design options within Pareto frontiers. Results demonstrated that horizontal louver by 128.52 % TCP, 15.10 % UDI improvement in south-oriented façade in BSk, and overhang with fin (OF) by 18.99 % EUI reduction in southern facade in BSh climates contribute the most to enhance the objective metrics. Also, ABBS was not a stand-first system to enhance all the objective metrics compared to the other shading systems in none of the examined climates. Sensitivity analysis indicated that WWR has the most significant positive impact on EUI compared to the other studied metrics in almost all design options.

A simple new approach predicting how microalgae culture systems will perform under sunlight and artificial light conditions

There are many problems associated with the methods for scaling microalgae culture systems. Mathematical models - predictive models in particular - are considered key, but most of them require specialist programming and computer science skills. Even where these skills are available, new parameters (such as radiative properties for determining the effect of light attenuation on photosynthetic growth) have to be determined with each application of the model for a new strain of microalgae, and this in itself is highly complex. Consequently, the methods used in the field are still mainly (semi-)empirical. A simplified method of determining maximal performance and the corresponding optimal operating points (i.e. biomass concentration, harvesting rate) for a given culture system, strain and culture condition (including solar) is presented here. The approach involves engineering equations, which are smartly adapted in this study to eliminate parameters that are difficult to obtain, with a few conventional small-scale experiments to determine the remaining key parameters relevant to the strain. The method is applied with two strains: Haematococcus pluvialis (in green phase and under continuous light) and a Picochlorum maculatum strain isolated from the Qatar desert (in both continuous and diurnal light cycles). A deviation of <10 % was achieved between the predicted data and the experimental data. The proposed method, which eliminates several months of experiments and is relatively simple to apply, appears to be an appropriate tool for optimizing and accelerating the scaling-up of microalgae culture systems.

Flue gas CO2 supply methods for microalgae utilization: A review

The potential for utilizing flue gas as a carbon source in microalgal cultivation holds great promise. Incorporating flue gas as a carbon source into microalgae culture processes can accelerate the growth rate of microalgae, consequently enhancing the overall economic viability of the integrated process. There are two key sources of flue gas to consider: flue gas from coal-fired power plants, characterized by a CO2 concentration of 12–15 w/w%, and flue gas from coal chemical processes, boasting a CO2 concentration of 90–99 w/w%. Additionally, the choice between an open or sealed microalgae culture system can also influence economic efficiency. Thus, there are four distinct microalgal cultivation routes to assess: in-situ open systems, off-situ open systems, in-situ sealed systems, and off-situ sealed systems. The incorporation of flue gas as a carbon source in microalgae cultivation demonstrates significant potential for reducing both environmental impact and costs, rendering it a highly promising and sustainable approach for economically efficient microalgae cultivation. In this review, the in-situ open route is recommended for the situation with high flue gas CO2 concentration and the target products of low-margin commodities, while the off-situ sealed route is suitable for the situation with low flue gas CO2 concentration and the target products of high value-added products.

Effective removing of rotifer contamination in microalgal lab-scale raceway ponds by light-induced phototaxis coupled with high-voltage pulse electroshock

Rotifer reproduction control in open microalgae cultivation systems poses a significant challenge for large-scale industries. Conventional methods, such as electric, meshing, and chemical techniques, are often expensive, ineffective, and may have adverse environmental-health impacts. This study investigated a promising control technique through light-induced phototaxis to concentrate rotifers in a specific spot, where they were electroshocked by local-limited exposure dose. The results showed that the rotifers had the most pronounced positive and negative phototropism with phototaxis rates of 66.7 % and −78.8 %, respectively, at blue-light irradiation of 30 µmol∙m−2∙s−1 and red-light irradiation of 22.5 µmol∙m−2∙s−1 for 20 min. The most effective electroshock configuration employed 1200 V/cm for 15 min with a 1-second cycle time and a 10 % duty cycle, resulting in a 75.0 % rotifer removal rate without impacting microalgae growth. The combination of the two light beams could effectively lead rotifers to designated areas where they were electrocuted successfully.

Technological mapping of led lighting for microalgae: a survey of patent deposits

Microalgae are a significant focus in bioprocess research due to their capacity to capture atmospheric CO2 and produce valuable secondary metabolites. Light-emitting diodes (LEDs) have gained prominence as a light source for microalgae, offering advantages over traditional lighting due to their narrowband spectrum, along with many advantages in comparison to traditional light sources. This study examines 578 patent families related to microalgae processes with LED illumination, assessing light spectrum, microalgae species, and bioproduct yield, as well as their connection to current scientific literature. The patent analysis was conducted using Orbit Intelligence® software, and scientific research was validated using the Elsevier Scopus® database, meeting specified search criteria. Both technological and scientific advancements have occurred over the past decade. Over the last two decades, the majority of patent activity has been centered in Asia, with China leading with 333 patent filings, followed by South Korea (96) and the USA (45). However, the scientific publication landscape tells a different story, with the USA leading in publications (1693), followed by China (771), and Germany (713). Most patents relate to microalgae cultivation systems. The primary microalgae species featured in the patents include Chlorella, Arthrospira (Spirulina), and Haematococcus, with biodiesel being the most prominent bioproduct. Regarding LED light quality, the patents predominantly utilize a combination of red and blue lights in various ratios. In summary, the adoption of LED lighting in microalgae production has grown significantly in both the industrial and scientific sectors. Tailoring light emissions in microalgae cultivation proves to be a smart and cost-effective method for enhancing efficiency, and this trend is expected to continue expanding in the future.

Current challenges of microalgae applications: exploiting the potential of non-conventional microalgae species.

The intensified attention to health, the growth of an elderly population, the changing lifestyles, and the medical discoveries have increased demand for natural and nutrient-rich foods, shaping the popularity of microalgae products. Microalgae thanks to their metabolic versatility represent a promising solution for a 'green' economy, exploiting non-arable land, non-potable water, capturing carbon dioxide (CO2) and solar energy. The interest in microalgae is justified by their high content of bioactive molecules, such as amino acids, peptides, proteins, carbohydrates, polysaccharides, polyunsaturated fatty acids (as ω-3 fatty acids), pigments (as β-carotene, astaxanthin, fucoxanthin, phycocyanin, zeaxanthin and lutein), or mineral elements. Such molecules are of interest for human and animal nutrition, cosmetic and biofuel production, for which microalgae are potential renewable sources. Microalgae, also, represent effective biological systems for treating a variety of wastewaters and can be used as a CO2 mitigation approach, helping to combat greenhouse gases and global warming emergencies. Recently a growing interest has focused on extremophilic microalgae species, which are easier to cultivate axenically and represent good candidates for open pond cultivation. In some cases, the cultivation and/or harvesting systems are still immature, but novel techniques appear as promising solutions to overcome such barriers. This review provides an overview on the actual microalgae cultivation systems and the current state of their biotechnological applications to obtain high value compounds or ingredients. Moreover, potential and future research opportunities for environment, human and animal benefits are pointed out. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Progress of Algaculture technology as Biofuel

As the problem of climate change becomes more and more serious, improving the carbon emission situation will be an effective way to solve various climate problems. Algal biofuels can build a complete carbon cycle and reduce carbon emissions. In this paper, the conditions required for microalgae cultivation and how diverse factors play a role in the growth rate and yield of microalgae were reviewed. And show how different factors like pH value, illumination, temperature, medium of culture, and nutrients in the medium affect the content of different substances in the final microalgae to help breed the matching microalgae. Macroscopically, this paper introduces both open raceway ponds and photobioreactors as microalgae cultivation systems and their respective characteristics. Microalgae will produce higher lipid content and hydrogen production capacity in different acid-base environments, respectively, and provide a nitrogen-deficient environment to further increase lipid production. The two-stage hybrid system combining an open raceway pond and photobioreactor has more obvious advantages in terms of growth rate and yield.

Development of dual strain microalgae cultivation system for the direct carbon dioxide utilization of power plant flue gas

This study aims to propose a biological system that allows for direct utilization of flue gas for carbon dioxide capture and utilization by microalgae. The strain Chlorella sp. ABC-001 is employed for its high growth rate as well as lipid and carbohydrate content. Toxicity tests showed that cell growth was unaffected by NO, but the presence of SO2 showed critical damage on cell growth. Hence, an extremophile alga, Galdieria sulphuraria 5587.1 was applied to build a dual-strain cultivation system to mitigate the effect of SO2 toxicity and increase CO2 capture efficiency. All SO2 was removed by Galdieria culture and the system exhibited stable growth from a simulated flue gas stream containing CO2, NO and SO2. Combined CO2 biofixation rate of 793 mg/L/d and lipid productivity of 113 mg/L/d was achieved. The results showed that this new cultivation system is a promising alternative for reducing CO2 emissions from power plants.

Transparent antibiofouling coating to improve the efficiency of Nannochloropsis gaditana and Chlorella sorokiniana culture photobioreactors at the pilot-plant scale

The implementation of industrial-scale facilities for microalgae cultivation is limited due to the high operation costs. One of the main problems in obtaining an efficient and long-lasting microalgae culture system is biofouling. The particular issue when developing antibiofouling surfaces for microalgae cultures is that the material must be transparent. The main purpose of this work was to evaluate the antibiofouling efficiency of a non-toxic polydimethylsiloxane-based coating prepared with polyethylene glycol-based copolymer on different photobioreactors at the pilot-plant scale. The antifouling properties result from the development of a fouling-release coating utilizing hydrogel technology. Nannochloropsis gaditana and Chlorella sorokiniana were cultured outdoors for 3 months over the summer, when biofouling formation is at its highest due to environmental conditions, to test the coating's antibiofouling efficiency. Although biofouling was not completely prevented in either photobioreactor, the coating significantly reduced cell adhesion compared to the polydimethylsiloxane control (70% less adhesion). Therefore, this coating was shown to be a good alternative for constructing efficient closed-photobioreactors at the pilot-plant scale, at least for cultures lasting 3 months.

Evaluating optimal cultivation sites for microalgae as a sustainable biofuel energy resource

Microalgae are promising candidates for the production of sustainable biofuels due to their high productivity, ability to grow in diverse environments, and carbon capture potential. However, the selection of suitable cultivation sites is crucial to achieve efficient and sustainable microalgae cultivation for biofuel production. This study discusses the evaluation of optimal cultivation sites for microalgae as a sustainable biofuel energy resource, with a focus on the naturally favorable conditions for microalgae cultivation in district Gwadar, Pakistan. The global demand for renewable energy sources has increased due to the rising energy crisis, and microalgae has emerged as an efficient source of biofuel on a large scale. The study utilizes geographic information systems to highlight suitable microalgae cultivation areas in district Gwadar. The results indicate that district Gwadar has the potential to overcome the energy crisis by cultivating microalgae as a biofuel energy source. The research provides a promising idea for designing a master plan to install a microalgae cultivation system in the highlighted sites for the concerned authorities.

Microalgal cultivation in open and closed systems under a tropical climate: A life cycle comparison

Microalgae can grow in different cultivation media, including domestic sewage, and have been used in several applications, including as biofertilizer in agriculture. Since microalgae have a cellular mechanism capable of using solar energy to synthesize bio-based compounds, the geographical location and appropriate climate conditions, such as radiation intensity and high temperature, increase the productivity and performance of cultivation systems. However, there are still few studies on microalgal cultivation in tropical areas, mainly to recover nutrients from domestic sewage for agricultural reuse of the biomass generated as biofertilizers. Aiming to overcome this lack of studies and to identify more environmentally sustainable solutions, life cycle assessment (LCA) method was employed to evaluate the potential environmental impacts of these microalgae cultivation systems. In this context, the aim of this study was to compare the potential environmental impacts of two systems: i) a raceway pond and ii) a flat panel photobioreactor on a pilot scale. Both systems were designed for microalgae cultivation under tropical climate conditions using domestic sewage anaerobically treated. The results indicated that the microalgae cultivation scenario in raceway pond was the worst option for the assessed impact categories. Sensitivity analysis showed that electricity consumption had a large influence on environmental impact while the use of inoculum did not significantly influence. Thus, electricity consumption increased the environmental impacts in most of the evaluated categories, however, the tropical climate conditions favored a decrease in the use of this input, helping to reduce the environmental impacts of the studied systems.

Sustainability assessment of a conceptual multipurpose offshore platform in the South China Sea

An offshore multipurpose floating platform (MPFP) combines different marine technologies to serve industry needs using one infrastructure; the aim of an MPFP is enlarging the synthesis benefits and reducing the negative impacts. Ocean thermal energy conversion (OTEC) in particular has attracted significant attention for its great potential and low environmental risk. This research demonstrates the system design of a conceptual MPFP in the South China Sea, evaluating its economic and environmental sustainability using an inclusive index. The system is based on a modular floating structure with a designed lifetime of 50 years. Tuna aquaculture, microalgae cultivation and processing, and the OTEC energy infrastructure are integrated to increase the profitability of the applications. We adopted a high-yield photobioreactor microalgae cultivation system and a low-cost barge-type floating structure combined with a semisubmergible to reduce the required area and cost of the floating structure and improve the sustainability of the system. The inclusive impact index “Triple-I-light (IIIlight)” was calculated to evaluate the environmental sustainability and economic feasibility of the floating system. The result shows that the new system becomes environmentally neutral (EF = BC) at a lifetime of 11.5 years, showing sustainability (IIIlight ≤ 0) at a lifetime of 20 years. The proposed system can produce fish with no external energy or feed supply. An autonomous system, such as the one proposed here, is considered very effective when it comes to utilizing the ocean and contributing to a sustainable society.

System modeling of the thermal behavior of a building equipped with facade-integrated photobioreactors: Validation and comparative analysis

A microalgae-based biofacade is a double-skin facade with integrated photobioreactors (PBRs) for solar cultivation of microalgae. One purpose of cultivating microalgae on building facades is to create an ‘active’ facade that can reduce the energy consumption required to maintain thermal comfort inside the building, by creating exchange loops between the building and the microalgae cultivation system. This paper describes the experimental validation of a thermal model of a building equipped with a biofacade PBR, and its use in analyzing the energy consumption of international-standard buildings in two different climate conditions (oceanic climate using the weather data from Nantes, France, and semi-arid climate using weather data from Los Angeles, USA). The results of this analysis show that the microalgae biofacade almost entirely removes the building's cooling demand, and the warmer the location the stronger the effect. In addition, the heating demand of the biofacade PBR is also greatly reduced (between 92% and 100% depending on the location). The microalgae biofacade is therefore a more sustainable solution to produce valuable biomass and a promising technology for the development of zero-emission buildings; to this end, suggestions for optimizing the process are put forward.

Biogas‐Assisted Growth of Chlorella vulgaris in an Open Raceway Pond: Proof of Concept and Economic Assessment

AbstractMicroalgae are a promising source of renewable fuels and value‐added chemicals but are hampered by the high cost of cultivation. Sparging CO2 in water bodies can increase the yield and reduce the cost. This work studies the feasibility of synergistically integrating a biogas digester with a microalgae cultivation system. Raw biogas is sparged through the microalgal culture to increase the microalgae yield while also purifying biogas for further use. At laboratory scale, Chlorella vulgaris was cultivated in a 1‐L batch, and biogas was sparged for 3 h per day. For a demonstration‐scale 4000‐L raceway pond, a special contacting device was used to supply biogas to the microalgal culture. On the laboratory scale, C. vulgaris concentration increased by 30 % when sparged with biogas. Moreover, the protein and lipid contents were higher than in an air‐sparged control batch. The cultivation cost of microalgae decreased by 20 %.