Micropatterned Surfaces Research Articles

Finite Element Simulations and Statistical Analysis for the Tribological Design of Mutually Textured Surfaces and Related Experimental Validation

Abstract In the last few decades, micro-texturing has become a widely studied technique to modify the frictional behavior between surfaces in both dry and lubricated regimes. Among all the available techniques, the laser surface texturing appears to be fast, clean, and flexible, and thus a good candidate in realizing surface micro-patterns, also for the improvement of the tribological performance of automotive components when subjected to dry friction. For this reason, in the present work, the tribological response of four different patterns of micro-holes on two contrasting materials, specifically silicon carbide and carbon black have been investigated with a coupled experimental–numerical approach. The static and the dynamic friction coefficients have been extracted from the 25 different combinations of these surface textures including the flat counterparts. Then, the influence of the holes diameter, their density, and the material has been studied thanks to a multivariate linear regression. Specifically, it emerged that, in a dry regime, the most emerging parameter is the micro-holes diameter, for both static and dynamic frictions. Moreover, for both static and dynamic frictions, the material which more influences the effects of patterns to the overall frictional behavior is here the stiffest one. These insights for the design of micro-patterned surfaces with controlled frictional properties could be useful for those applications in which a dry friction regime is present.

Frictional Properties of Biomimetic Micro-Hexagonal-Textured Surfaces Interacting with Soft Counterfaces under Dry and Wet Conditions.

Biomimetic micro-hexagonal-textured surfaces have sparked interest for their application in fields that demand high friction and adhesion, such as micro-robotics and biomedicine. Despite extensive research conducted on this specific microstructure, its friction behavior against soft counterfaces remains a topic that has not been fully investigated yet. This study examines how micro-hexagon textures behave when they come into contact with engineered and biological materials like gelatin and chicken skin in dry and wet conditions. The results show clearly that under dry contact conditions, flat surfaces generate higher friction compared to hexagon micropattern surfaces. Under wet conditions, hexagon micropattern surfaces generate higher friction compared to flat surfaces. In wet conditions specifically, the static coefficient of friction is up to 13 times greater than that of a flat specimen against glass, up to 11 times greater against gelatin, and up to 6 times greater against chicken skin. For the dynamic coefficient of friction, the patterned surface demonstrates a maximum increase by a factor of 28 against glass, 11 against gelatin, and 5 against chicken skin. These results further develop our knowledge of these hexagon micropattern surfaces and pave the way for their utilization in future technological advancements in which soft and wet counterfaces are to be considered, such as in biomedical applications that can benefit from increased friction in wet conditions for better control and stability.

Decoration of Nanoenabled Optoplasmonic Sensors on Flexible Micropatterned Surfaces

Decoration of Nanoenabled Optoplasmonic Sensors on Flexible Micropatterned Surfaces

Influence of Initial Film Properties in UVO-Mediated Patterning of an Elastomeric Film Using a TEM Grid.

Ultraviolet irradiation of a cross-linked polydimethylsiloxane (PDMS) Sylgard 184 film in the presence of atmospheric oxygen (UVO) through a bare transmission electron microscope (TEM) sample holding grid is a rather simple and widely utilized technique for creating micropatterned surfaces. The surface oxidation of a Sylgard 184 film due to UVO exposure is associated with densification and the formation of a silica-like surface layer, which under a TEM grid happens only over the exposed areas of the film, resulting in a physicochemical pattern. It is known that the depth (hD) of the features depends on the duration of UVO exposure (tE). In this article, we show for the first time that hD also depends on the initial film thickness (hF) and the cross-linker percentage (CL, ratio of part A to part B) in a Sylgard 184 thin film. We show that for a specific tE, hD progressively decreases with the reduction in hF. On the other hand, hD shows a nonmonotonic dependence with CL, resulting in patterns with maximum depth for CL ≈ 10.0%. We attribute this observation to the combined effect of resistance against the penetration of the propagation front by the rigid substrate as well as stress relaxation within the exposed parts of the film below the propagating front in films with higher CL values leading to the variation of hD. The observation reported here would allow the potential fabrication of polymer films with physicochemical patterns with feature height on demand by a one-step, facile technique.

Roles of Micropillar Topography and Surface Energy on Cancer Cell Dynamics

Microstructured surfaces are renowned for their unique properties, such as waterproofing and low adhesion, making them highly applicable in the biomedical field. These surfaces play a crucial role in influencing cell response by mimicking the native microenvironment of biological tissues. In this study, we engineered a series of biomimetic micropatterned surfaces using polydimethylsiloxane (PDMS) to explore their effects on primary breast cancer cell lines, contrasting these effects with those observed on conventional flat surfaces. The surface topography was varied to direct cells’ attachment, growth, and morphology. Our findings elucidate that surface-free energy is not merely a background factor but plays a decisive role in cell dynamics, strongly correlating with the spreading behaviour of breast cancer cells. Notably, on micropillar surfaces with high surface-free energy, an increase in the population of cancer cells was observed. Conversely, surfaces characterised by lower surface-free energies noted a reduction in cell viability. Moreover, the structural parameters, such as the gaps and diameters of the pillars, were found to critically influence cellular dispersion and adherence, underscoring the importance of the microstructures’ topography in biomedical applications. These insights pave the way for designing advanced microstructured surfaces tailored to specific cellular responses, opening new avenues for targeted cancer therapies and tissue engineering.

Implementation of high‐performance, freestanding flexible film masks through photosensitive polyimide for arbitrary surface micropatterns creation

AbstractGiven the widespread presence of intricate surfaces, the development of electronics has generated a significant demand for surface patterning techniques capable of creating refined or novel patterns. Nevertheless, present surface patterning techniques suffer from complex processes, limited resolution, stringent conditions, and high manufacturing costs. Herein, we present a novel approach for arbitrary surface micropatterning using photosensitive polyimide (PSPI), enabling the in situ fabrication of electrodes without the need for a pattern‐transferring process. On this basis, we have implemented a high‐performance, freestanding flexible thin‐film mask with high optical transparency that facilitates precise alignment of microelectrode patterns with the target material. It also exhibits exceptional mechanical properties suitable for long‐term use and high‐temperature applications, with a notable glass transition temperature of up to 300°C. The fabricated masks with thicknesses of 5–20 μm are well‐suited for high‐resolution applications, including those requiring sub‐5 μm resolution. Furthermore, the creation of microelectrodes on a variety of surfaces utilizing the fabricated PSPI masks was successfully demonstrated. Our facile method provides a solid foundation for achieving efficient micropatterning for the fabrication of high‐performance flexible electronics on complex surfaces.

Membrane localization of actin filaments stabilizes giant unilamellar vesicles against external deforming forces

Actin organization is crucial for establishing cell polarity, which influences processes such as directed cell motility and division. Despite its critical role in living organisms, achieving similar polarity in synthetic cells remains challenging. In this study, we employ a bottom-up approach to investigate how molecular crowders facilitate the formation of cortex-like actin networks and how these networks localize and organize based on membrane shape. Using giant unilamellar vesicles (GUVs) as models for cell membranes, we show that actin filaments can arrange along the membrane to form cortex-like structures. Notably, this organization is achieved using only actin and crowders as a minimal set of components. We utilize surface micropatterning to examine actin filament organization in deformed GUVs adhered to various pattern shapes. Our findings indicate that at the periphery of spherical GUVs, actin bundles align along the membrane. However, in highly curved regions of adhered GUVs, actin bundles avoid crossing the highly curved edges perpendicular to the adhesion site and instead remain in the lower curved regions by aligning parallel to the micropatterned surface. Furthermore, the actin bundles increase the stiffness of the GUVs, effectively counteracting strong deformations when GUVs adhere to micropatterns. This finding is corroborated by real-time deformability cytometry on GUVs with synthetic actin cortices. By precisely manipulating the shape of GUVs, our study provides a minimal system to investigate the interplay between actin structures and the membrane. Our findings provide insights into the spatial organization of actin structures within crowded environments, specifically inside GUVs that resemble the size and shape of cells. This study advances our understanding of actin network organization and functionality within cell-sized compartments.

Biologicals and Biomaterials for Corneal Regeneration and Vision Restoration in Limbal Stem Cell Deficiency.

The mammalian cornea is decorated with stem cells bestowed with the life-long task of renewing the epithelium, provided they remain healthy, functional, and in sufficient numbers. If not, a debilitating disease known as limbal stem cell deficiency (LSCD) can develop causing blindness. Decades after the first stem cell (SC) therapy is devised to treat this condition, patients continue to suffer unacceptable failures. During this time, improvements to therapeutics have included identifying better markers to isolate robust SC populations and nurturing them on crudely modified biological or biomaterial scaffolds including human amniotic membrane, fibrin, and contact lenses, prior to their delivery. Researchers are now gathering information about the biomolecular and biomechanical properties of the corneal SC niche to decipher what biological and/or synthetic materials can be incorporated into these carriers. Advances in biomedical engineering including electrospinning and 3D bioprinting with surface functionalization and micropatterning, and self-assembly models, have generated a wealth of biocompatible, biodegradable, integrating scaffolds to choose from, some of which are being tested for their SC delivery capacity in the hope of improving clinical outcomes for patients with LSCD.

Energizing geriatric healthcare: A triboelectric energy harvester with self-powered morse code generator and IoT-Enabled remote sensing tactile patch

Self-powered tactile sensors unleash a diverse array of distinctive interaction techniques and other possibilities in the contemporary world of small-scale electronics. In many circumstances of information exchange, the touch sensor is a fundamental and crucial human-machine interface (HMI). Rapid advancements in portable, wearable, and Internet of Things technologies demand a tactile sensing system to be thin, flexible, effective, self-powered, and secure. Triboelectric nanogenerator (TENG) based touch sensors are the future of flexible and self-powered sensors. Triboelectric nanogenerator based sensors exhibit excellent material compatibility and can produce substantial electrical signals even when frequencies are low and forces are moderate; this motivates researchers to develop self-powered triboelectric sensors. Here, we have proposed a skin-conforming and flexible tactile sensor with a micro-patterned dielectric surface, driven by a stencil-printed activated carbon electrode (Printed carbon PC-TENG). The device performance has been enhanced through the optimization of its output, achieved by analysing its structure using Finite Element Analysis in conjunction with the electrostatic module of COMSOL Multiphysics. The fabricated device produces a voltage in an open circuit measuring 242V along with a power density of 14.69 μW/cm2 in single electrode mode. Further, it can be employed as an energy harvester, demonstrated through energizing LEDs, LCD, Wristwatches, and digital ring counter. The proposed TENG can also serve as an international Morse code generator and self-powered sensor for IoT-enabled remote-sensing finger patches, providing medical assistance to geriatric and bedridden patients.

Regulation of mitochondrial network architecture and function in mesenchymal stem cells by micropatterned surfaces

Abstract Mitochondrial network architecture, which is closely related to mitochondrial function, is mechanically sensitive and regulated by multiple stimuli. However, the effects of microtopographic cues on mitochondria remain poorly defined. Herein, polycaprolactone (PCL) surfaces were used as models to investigate how micropatterns regulate mitochondrial network architecture and function in rat adipose-derived stem cells (rASCs). It was found that large pit (LP) induced rASCs to form larger and more complex mitochondrial networks. Consistently, the expression of key genes related to mitochondrial dynamics revealed that mitochondrial fusion (MFN1 and MFN2) and midzone fission (DRP1 and MFF) were increased in rASCs on LP. In contrast, the middle pit (MP) enhanced mitochondrial biogenesis, as evidenced by the larger mitochondrial area and higher expression of PGC-1. Both LP and MP promoted ATP production in rASCs. It is likely that LP increased ATP levels through modulating mitochondrial network architecture while MP stimulated mitochondria biogenesis to do so. Our study clarified the regulation of micropatterned surfaces on mitochondria, highlighting the potential of LP and MP as a simple platform to stimulate mitochondria and the subsequent cellular function of MSCs.

Long‐Term Immersion Study for Durability of Interconnected Micropatterned Surfaces with Sustained Water Repellency

AbstractThe sustained water repellency of interconnected micropatterned surfaces is explored over an extended duration, with a focus on their resilience during a 90‐day water‐immersion test. Initially, the microstructure surfaces exhibit high water repellency, a characteristic of the Cassie–Baxter state. However, subsequent detailed temporal analyses reveal varying responses depending on the structural topology. The interconnected micropatterned surfaces exhibit remarkable long‐term resistance to water; this is attributed to the formation of large and stable air pockets enabled by their unique microcavity structures. In comparison, hierarchical microcavity surfaces with micropillars exhibit a notable decrease in water repellency, as evidenced by reduced contact angles, suggesting a transition to a wetting state owing to the emergence of surface hydrophilicity during long‐term water exposure. This study demonstrates the importance of stable air‐pocket effects, particularly in applications where the long‐term stability of liquid repellency is critical, and suggests the role of interconnected structures in maintaining water repellency over time.

Manipulating Stem Cell Fate with Disordered Bioactive Cues on Surfaces: The Role of Bioactive Ligand Selection.

The development of 2D or 3D bioactive platforms for rapidly isolating pure populations of cells from adult stem cells holds promise for advancing the understanding of cellular mechanisms, drug testing, and tissue engineering. Over the years, methods have emerged to synthesize bioactive micro- and nanostructured 2D materials capable of directing stem cell fate. We introduce a novel method for randomly micro- or nanopatterning any protein/peptide onto both 2D and 3D scaffolds via spray technology. Our goal is to investigate the impact of arranging bioactive micropatterns (ordered vs disordered) on surfaces to guide human mesenchymal stem cell (hMSC) differentiation. The spray technology efficiently coats materials with controlled, cost-effective bioactive micropatterns in various sizes and shapes. BMP-2 mimetic peptides were covalently grafted, individually or in combination with RGD peptides, onto activated polyethylene terephthalate (PET) surfaces through a spraying process, incorporating nano/microscale parameters like size, shape, and composition. The study explores different peptide distributions on surfaces and various peptide combinations. Four surfaces were homogeneously functionalized with these peptides (M1 to M4 with various densities of peptides), and six surfaces with disordered micro- and nanopatterns of peptides (S0 to S5 with different sizes of peptide patterns) were synthesized. Fluorescence microscopy assessed peptide distribution, followed by hMSC culture for 2 weeks, and evaluated osteogenic differentiation via immunocytochemistry and RT-qPCR for osteoblast and osteocyte markers. Cells on uniformly peptide-functionalized surfaces exhibited cuboidal forms, while those on surfaces with disordered patterns tended toward columnar or cuboidal shapes. Surfaces S4 and S5 showed dendrite-like formations resembling an osteocyte morphology. S5 showed significant overexpression of osteoblast (OPN) and osteocyte markers (E11, DMP1, and SOST) compared to control surfaces and other micropatterned surfaces. Notably, despite sharing an equivalent quantity of peptides with a homogeneous functionalized surface, S5 displayed a distinct distribution of peptides, resulting in enhanced osteogenic differentiation of hMSCs.

Micropatterning of biologically derived surfaces with functional clay nanotubes

ABSTRACT Micropatterning of biological surfaces performed via assembly of nano-blocks is an efficient design method for functional materials with complex organic–inorganic architecture. Halloysite clay nanotubes with high aspect ratios and empty lumens have attracted widespread interest for aligned biocompatible composite production. Here, we give our vision of advances in interfacial self-assembly techniques for these natural nanotubes. Highly ordered micropatterns of halloysite, such as coffee rings, regular strips, and concentric circles, can be obtained through high-temperature evaporation-induced self-assembly in a confined space and shear-force brush-induced orientation. Assembly of these clay nanotubes on biological surfaces, including the coating of human or animal hair, wool, and cotton, was generalized with the indication of common features. Halloysite-coated microfibers promise new approaches in cotton and hair dyeing, medical hemostasis, and flame-retardant tissue applications. An interfacial halloysite assembly on oil microdroplets (Pickering emulsion) and its core–shell structure (functionalization with quantum dots) was described in comparison with microfiber nanoclay coatings. In addition to being abundantly available in nature, halloysite is also biosafe, which makes its spontaneous surface micropatterning prospective for high-performance materials, and it is a promising technique with potential for an industrial scale-up.

Generating human skeletal myoblast spheroids for vascular myogenic tissue engineering

Engineered myogenic microtissues derived from human skeletal myoblasts offer unique opportunities for varying skeletal muscle tissue engineering applications, such as in vitro drug-testing and disease modelling. However, more complex models require the incorporation of vascular structures, which remains to be challenging. In this study, myogenic spheroids were generated using a high-throughput, non-adhesive micropatterned surface. Since monoculture spheroids containing human skeletal myoblasts were unable to remain their integrity, co-culture spheroids combining human skeletal myoblasts and human adipose-derived stem cells were created. When using the optimal ratio, uniform and viable spheroids with enhanced myogenic properties were achieved. Applying a pre-vascularization strategy, through addition of endothelial cells, resulted in the formation of spheroids containing capillary-like networks, lumina and collagen in the extracellular matrix, whilst retaining myogenicity. Moreover, sprouting of endothelial cells from the spheroids when encapsulated in fibrin was allowed. The possibility of spheroids, from different maturation stages, to assemble into a more large construct was proven by doublet fusion experiments. The relevance of using three-dimensional microtissues with tissue-specific microarchitecture and increased complexity, together with the high-throughput generation approach, makes the generated spheroids a suitable tool for in vitro drug-testing and human disease modeling.

Surface micropatterning of 3D printed PCL scaffolds promotes osteogenic differentiation of BMSCs and regulates macrophage M2 polarization

Micropatterned structures on the surface of materials possessing biomimetic properties to mimic the extracellular matrix and induce cellular behaviors have been widely studied. However, it is still a major challenge to obtain internally stable and controllable micropatterned 3D scaffolds for bone repair and regeneration. In this study, 3D scaffolds with regular grating arrays using polycaprolactone (PCL) as a matrix material were prepared by combining 3D printing and soft lithography, and the effects of grating micropatterning on osteogenic differentiation of BMSCs and M1/M2 polarization of macrophages were investigated. The results showed that compared with the planar group and the 30um grating spacing group, PCL with a grating spacing of 20um significantly promoted the osteogenic differentiation of BMSCs, induced the polarization of RAW264.7 cells toward M2 type, and suppressed the expression of M1-type pro-inflammatory genes and markers. In conclusion, we successfully constructed PCL-based three-dimensional scaffolds with stable and controllable micrographs (grating arrays) inside, which possess excellent osteogenic properties and promote the formation of an immune microenvironment conducive to osteogenesis. This study is a step forward to the exploration of bone-filling materials affecting cell behavior, and makes a new contribution to the provision of high-quality materials.

Interplay of fluid rheology and micro-patterning toward modulating draining characteristics on an inclined substrate

We investigate a gravity-driven thin film flow of a non-Newtonian liquid over an inclined micro-patterned surface. We demonstrate the effect of micro-patterning on the film draining rate and the velocity profile by varying the relative slit width (Tr) and the length of the periodic irregularities (L). We unveil the interplay of the substrate structure and the fluid rheology by modeling the non-Newtonian thin film using the Carreau model, and the rheology of the film is varied for different values of power index n. Through numerical simulations, we delineate the effects of inertia, viscous, and capillary forces on the physics of thin film flow. We report a significant augmentation of flow velocity for both shear-thinning and shear-thickening fluids as a result of substrate micro-patterning, with the relative slit width playing a dominant role while the length of the periodic irregularities has only a minor influence on drainage characteristics. However, when the sole effect of fluid rheology is considered, flow velocity enhances for pseudoplastic fluid and decreases for dilatant fluid in comparison to Newtonian fluid. We examine the combined effect of rheology and substrate topography, revealing the dominating influence of micro-patterning at high slit-widths, while the fluid rheology has a greater role to play at lower slit-widths. We also demonstrate that the susceptibility of flow physics on varying rheology or topography is greatest for low viscosity liquids. Finally, we mark different regimes where the augmentation of average velocity and surface velocity are individually achieved. Hence, we propose a suitable combination of substrate structure and fluid rheology to engineer a flow characteristic. Based on the suitability for various applications, we provide the key to simultaneously optimizing the fluid rheology and substrate micro-patterning for precise engineering and controlling the draining characteristics of a thin film.

Microfabricated Nitinol Stent Retrievers with a Micro-Patterned Surface.

Stent retrievers are medical devices that are designed to physically remove blood clots from within the blood vessels of the brain. This paper focuses on microfabricated nitinol (nickel-titanium alloy) stent retrievers, which feature micro-patterns on their surface to enhance the effectiveness of mechanical thrombectomy. A thick film of nitinol, which was 20 µm in thickness, was sputtered onto a substrate with a micro-patterned surface, using electroplated copper as the sacrificial layer. The nitinol film was released from the substrate and then thermally treated while folded into a cylindrical shape. In vitro experiments with pig blood clots demonstrated that the micro-patterns on the surface improved the efficacy of blood clot retrieval.

Preparation of PVDF/PVA composite films with micropatterned structures on light-cured 3D printed molds for hydrophilic modification of PVDF

Abstract Polyvinylidene fluoride (PVDF) is widely used in biotechnology due to its excellent biocompatibility, high temperature and pressure resistance, and outstanding mechanical properties. However, the hydrophobic nature of PVDF surface hinders the attachment of biological proteins. In order to enhance the wettability of PVDF surfaces, this study prepared composite films by blending PVDF with polyvinyl alcohol (PVA), and micro-patterned structures were fabricated on the material surface using a mold-replication method based on digital light processing (DLP) photopolymerization printing technology. A series of characterization techniques including surface morphology analysis, chemical composition analysis, and wettability testing were employed. The surface morphology analysis results indicated that the method of using DLP photopolymerization technology to print mold replicas and create micro-patterned structures was indeed effective in creating micro-patterned structures on both PVDF and PVDF/PVA composite films. The chemical composition analysis showed that the spin-coating of PVDF powder material resulted in PVDF β-phase crystalline structure, which has a positive effect on cell growth. Furthermore, the introduction of hydrophilic groups was achieved by mixing PVDF with PVA. Wetting test results indicate that the incorporation of the hydrophilic material PVA and micro-patterned surfaces both contribute to the improved hydrophilicity of the material. The water contact angle of the micro-patterned PVDF/PVA composite film reached 30.8°, exhibiting excellent hydrophilic properties. This study achieved the optimization of PVDF surface properties through micro-patterned surface modification and material composition design, providing novel insights for the further development of PVDF materials in the field of biotechnology.

Digital light processing technique aided 3D printing hierarchical super-wetting mesh coated with hydrophobic SiO2 for highly efficient oil-water separation

Gravity-driven filtration using super-wetting mesh surfaces holds significant promise in addressing oil pollution resulting from human activities. However, conventional techniques need help fabricating efficient separation mesh structures due to their limited stability in oily-water solutions. To overcome this, we employed a Digital Light Processing (DLP) 3D printing technique to fabricate a mesh with a micro-patterned surface using a UV light-curable polyurethane (PU). A post-UV curing step was implemented to transform the soft segments of PU into a solid state. To enhance the super-wetting properties, hydrophobic modified silica (m-SiO2)/UV curable polydimethylsiloxane (PDMS) solution was applied through dip coating, resulting in a hierarchical structure with high surface roughness and superhydrophobic characteristics. In oil–water separation tests, the hierarchical superhydrophobic mesh, featuring a pore size of 300 µm, demonstrated exceptional performance, achieving a remarkable flow flux of 37,410 L/m2 h with a separation efficiency of 97.46 %. Furthermore, the separation efficiency remained consistently above 97.0 % under varying pH conditions and oil mixtures, highlighting the excellent water-repellence capacity of the hierarchical mesh developed in this study.

Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutation.

Beyond the observed alterations in cellular structure and mitochondria, the mechanisms linking rare genetic mutations to the development of heart failure in patients affected by desmin mutations remain unclear due in part, to the lack of relevant human cardiomyocyte models. To shed light on the role of mitochondria in these mechanisms, we investigated cardiomyocytes derived from human induced pluripotent stem cells carrying the heterozygous DESE439K mutation that were either isolated from a patient or generated by gene editing. To increase physiological relevance, cardiomyocytes were either cultured on an anisotropic micropatterned surface to obtain elongated and aligned cardiomyocytes, or as a cardiac spheroid to create a micro-tissue. Moreover, when applicable, results from cardiomyocytes were confirmed with heart biopsies of suddenly died patient of the same family harboring DESE439K mutation, and post-mortem heart samples from five control healthy donors. The heterozygous DESE439K mutation leads to dramatic changes in the overall cytoarchitecture of cardiomyocytes, including cell size and morphology. Most importantly, mutant cardiomyocytes display altered mitochondrial architecture, mitochondrial respiratory capacity and metabolic activity reminiscent of defects observed in patient's heart tissue. Finally, to challenge the pathological mechanism, we transferred normal mitochondria inside the mutant cardiomyocytes and demonstrated that this treatment was able to restore mitochondrial and contractile functions of cardiomyocytes. This work highlights the deleterious effects ofDESE439Kmutation, demonstrates the crucial role of mitochondrial abnormalities in the pathophysiology of desmin-related cardiomyopathy, and opens up new potential therapeutic perspectives for this disease.