Sustainable economic development serves society but requires taking over space, often at the expense of areas occupied by single trees or even parts of forest areas. Techniques for transplanting adult trees used in various conflict situations at the interface of economy and nature work as a tool for sustainable management of urbanized and industrial areas, as well as, in certain circumstances, forest or naturally valuable areas. This study aimed to evaluate the effectiveness of ground-penetrating radar (GPR) in determining the horizontal and vertical extent of tree root systems before transplantation. Employing this non-invasive method to map root system architecture aids in the appropriate equipment selection and helps define the dimensions and depth of trenches to minimize root damage during excavation. This study specifically focused on the root systems of wild service trees (Sorbus torminalis (L.) Crantz) found in a limestone mine area, where some specimens were planned to be transplanted, as the species is protected under law in Poland. The root systems were scanned with a ground-penetrating radar equipped with a 750 MHz antenna. Then, the root balls were dug out, and the root parameters and other dendrometric parameters were measured. The GPR survey and manual root analyses provided rich comparative graphic material. The number of the main roots detected by the GPR was comparable to those inventoried after extracting the stump. The research was carried out in problematic soil, causing non-standard deformations of the root systems. Especially in such conditions, identifying unusually arranged roots using the GPR method is valuable because it helps in a detailed planning of the transplanting process, minimizing root breakage during the activities carried out, which increases the survival chances of the transplanted tree in a new location.