Nonnutritive sweeteners are widely used as low-calorie replacements for nutritive sweeteners. Despite widespread use, it is unclear how nonnutritive sweeteners, when combined with exercise, influence plasma lipids and glucose. PURPOSE: To investigate the effects of nutritive and nonnutritive sweeteners on plasma lipid profiles, complete blood counts, and glucose content following exercise. METHODS: Ten healthy, college aged, individuals (4 females and 6 males) participated in the randomized, double-blinded, cross-over design study. Participants consumed 8 oz of sweetened drink, equivalent to three sodas worth of sweetener, containing either 445mg of stevia, 507mg of aspartame, 169mg of sucralose, or 110,000mg of sucrose in four separate occasions. After 30 minutes of each sweetened drink, participants completed a single bout of aerobic exercise on a cycle ergometer at 70% of HRmax for 45 minutes . Overnight fasting blood samples were collected at baseline, 30-min post-consumption of sweetened drink, and immediately post-exercise. RESULTS: Sucrose resulted in significantly higher glucose content (115.8 ± 6.14 mg/dL) than either sucralose (87.2 ± 7.09 mg/dL, p=.032) or stevia (86.1 ± 5.79 mg/dL, p=.010) at the 30-min post-consumption of the sweetened drink; however, this difference was no longer significant immediately post-exercise. Total cholesterol (TC), triglyceride (TG), and HDL-C were elevated following exercise (TC: 152.7 ± 9.66 to 158.6 ± 9.26 mg/dL, p<.001; TG: 69.5 ± 5.78 to 76.8 ± 5.83 mg/dL, p=.002; and HDL-C: 51.5 ± 2.47 to 53.9 ± 2.40 mg/dL, p<.001, respectively). Neutrophils increased (53.7± 2.67 to 59.1 ±1.93%, p=.012) and lymphocytes decreased (36.8 ± 2.56 to 31.3 ± 1.56%, p=.016) from baseline to thirty minutes post-consumption. CONCLUSIONS: The nonnutritive sweeteners did not change glucose content from baseline through exercise. However, a nutritive sweetener resulted in a spike in glucose 30-min post-consumption, which returned to baseline levels immediately after exercise, suggesting that glucose may be used as a substrate during exercise. Elevated neutrophils, paired with decreased lymphocytes, may be indicative of an acute immune response to exercise.