Mg Of Succinate Research Articles

Влияние цитофлавина на антиоксидантный статус пациентов с розацеа легкой и средней степени тяжести

Изучена антиоксидантная активность цитофлавина у пациентов c розацеа: 26 пациентов (женщины в возрасте от 26 до 54 лет, основная группа) на фоне стандартного лечения получали препарат цитофлавин (НТФФ «ПОЛИСАН», Санкт-Петербург, Россия) внутрь по 600 мг по сукцинату (2 таблетки) 2 раза в сутки в течение 21 дня; 20 пациентов (женщины в возрасте от 28 до 52 лет, контрольная группа) получали только стандартное лечение. Группу сравнения составили практически здоровые пациенты (21 женщина). Результаты исследования показали, что содержание гидроперекисей липидов и диеновых конъюгатов в плазме крови пациентов c розацеа до лечения было выше относительно группы сравнения в среднем на 25 %, малонового диальдегида — на 41 и 44 %, уровень церулоплазмина был ниже на 25 %, витамина Е — на 22 %, каталазы — в среднем на 13 % (p ≤ 0,05). Стандартная терапия практически не влияла на антиоксидантный статус пациентов с розацеа. Введение в схему лечения цитофлавина способствовало достоверному снижению в плазме крови пациентов гидроперекисей липидов на 17 %, диеновых конъюгатов — на 13 %, малонового диальдегида — на 18 %, по сравнению с больными контрольной группы (p ≤ 0,05). При анализе влияния цитофлавина на активность компонентов антиоксидантной системы установлено, что содержание церулоплазмина в крови пациентов после применения препарата выше аналогичного показателя до лечения на 44 %, витамина E — на 14 %, каталазы — на 24 % (p ≤ 0,05). Таким образом, включение цитофлавина в схему комплексного лечения розацеа следует считать патогенетически обоснованным и перспективным.

Polypurine Hairpins Directed against the Template Strand of DNA Knock Down the Expression of Mammalian Genes

We analyzed whether polypurine hairpins (PPRHs) had the ability to knock down gene expression. These hairpins are formed by two antiparallel purine domains linked by a loop that allows the formation of Hoogsteen bonds between both domains and Watson-Crick bonds with the target polypyrimidine sequence, forming triplex structures. To set up the experimental conditions, the human dhfr gene was used as a model. The PPRHs were designed toward the template strand of DNA. The transfection of the human breast cancer cell line SKBR3 with these template hairpins against the dhfr gene produced higher than 90% of cell mortality. Template PPRHs produced a decrease in DHFR mRNA, protein, and its corresponding enzymatic activity. In addition, the activity of DHFR PPRHs was tested against breast cancer cells resistant to methotrexate, observing high cell mortality. Given the difficulty in finding long polypyrimidine stretches, we studied how to compensate for the presence of purine interruptions in the polypyrimidine target sequence. The stability of PPRH was measured, resulting in a surprisingly long half-life of about 5 days. Finally, to test the generality of usage, template PPRHs were employed against two important genes involved in cell proliferation, telomerase and survivin, producing 80 and 95% of cell death, respectively. Taken together our results show the ability of antiparallel purine hairpins to bind the template strand of double strand DNA and to decrease gene transcription. Thus, PPRHs can be considered as a new type of molecules to modulate gene expression.

Effect of Ferric Iron on Siderophore Production and Pyrene Degradation by Pseudomonas fluorescens 29L

The effect of ferric iron [Fe(III)] on pyrene degradation and siderophore production was studied in Pseudomonas fluorescens 29L. In the presence of 0.5 microM of Fe(III) and 50 mg of pyrene per liter of medium as a carbon source, 2.2 mg of pyrene was degraded per liter of medium per day and 25.3 microM of 2,3-DHBA (2,3-dihydroxybenzoic acid) equivalent of siderophores was produced per day. However, the pyrene degradation rate was 1.3 times higher and no siderophores were produced with the addition of 1 microM of Fe(III). Similar trends were seen with 50 mg of succinate per liter of medium as a carbon source, although the growth of strain 29L and the succinate degradation rate were higher. In the absence of siderophore production, pyrene and succinate continued to be biodegraded. This indicates that Fe(III) and not siderophore production affects the hydrocarbon degradation rate. Only 18% of strain 29L mutants capable of growth on pyrene produced siderophores, while among the mutants capable of growth on succinate, only 10% produced siderophores. This indicates that siderophores are not required for pyrene biodegradation. Fe(III) enhances pyrene degradation in Pseudomonas fluorescens 29L but it may be utilized by mechanisms other than siderophores.