Bisphenols, parabens, benzophenone-3 and triclosan are synthetic chemicals commonly used in various products such as cosmetics, pharmaceuticals and food items, leading to human exposure through ingestion, dermal contact and inhalation. Once entering the body, they are rapidly absorbed, metabolised, and excreted in urine due to their short half-lives. Recognized as potential endocrine-disrupting chemicals (EDCs), they have raised concerns among researchers and regulatory bodies. In response to the concerns regarding environmental harm and risks, innovative analytical strategies are required based on the new criteria focused on the twelve principles of Green Analytical Chemistry. Therefore, a new methodology for the environmentally friendly, fast, efficient and simultaneous determination of nine potential EDCs in human urine, including three bisphenols (A, F and S), four parabens (methyl paraben, ethyl paraben, propyl paraben and butyl paraben), benzophenone-3 and triclosan, has been developed and validated. It is based on a dilute-and-shoot method and ultra-performance liquid chromatography coupled to tandem mass spectrometry analysis. The relative recoveries ranged from 80 % to 120 % with a precision lower than 20 % for all analytes. The concentration ranges obtained encompass the published levels of the compounds in urine and the limits of quantification were between 0.2 and 0.5 ng·mL−1. The proposed method is the most environmentally friendly and practical so far and it was successfully applied for the determination of these compounds in 20 human urine samples from adults residing in the Valencian Region (Spain), and initial findings suggest that methyl paraben, benzophenone-3 and ethyl paraben were the predominant compounds.