A request was received from the Secretariat of JCCA for explanation of the testing methods of specific components of cosmetics and it is believed that the term specific components refers to beauty components such as hormones and vitamins. However, the main components such as tar dyes and anticeptics will also be explained in this paper.The testing methods were divided into 5 main classifications and the following results were obtained from each.(1). Color Reaction and Colorimetric Analysis1. The change in solubility in water and CHC13 layers, color and fluorescence of the reaction products of 42 kinds of dyes and 9 kinds of non-ionic surface active agents in neutral, acidic and alkaline conditions were investigated and it was found that triphenyl methane series and xanthene series dyes, and Span 80 and Tween 80 surface active agents indicated different kinds of reactions.2. In an acidic solution, dyes such as methylene blue reacts with SO3″, S2O3″, CNS′, NO2′, Br′, BrO3′, I′, IO4′, C10′, C102′, C103′ and C104′ ions and become soluble in CHC13. This reaction can be utilized for quantitative analysis of the various ions but on the other hand, the presence of these ions obstructs separation and identification of the dyes and also, is the cause of errors in the Epton method which is a quantitative analytical method for anionic surface active agents.3. Permaton red can be easily detected in a mixture of permanent orange, toluidine red and permaton red by reaction with an alkali or NaBH4.4. The 6 kinds of reagents prepared by dissolving VOC13 and NH4VO3 in HOAc, methyl cellosolve solution of toluene-sulfonic acid and pyridine indicate characteristic coloring with phenols whose reactions solution, i.e., antiseptics, in aqueous are negative by FeC13 reagent antioxidants and hormones.(2). Paper Chromatography (PC) and Thin-Layer Chromatography (TLC)1. Analytical method by PC was developed for analyzing impurities in 13 kinds of mercapto compounds and 14 kinds of cold permanent was solutions.2. Very good separation of oil soluble dyes, and green and blue coloring matters was obtained by using Amberlite “Ion exchange resin papers” WA-2 and WB-2, and developing with acidic organic solvents.3. It was possible to identify components of 20 kinds of hair dyes containing amino or phenol group by TLC using silica gel, with benzene: diethylamine (9:1) as the solvent.4. TLC of 8 kinds of anti-histamine compounds was carried out with silica gel using BuOH:HOAc:H2O(5:1:4) as the solvent and it was possible to separate amino-ether groups which are permitted in the Pharmaceutical Affairs law of Japan from those which are not permitted.5. TLC of 16 kinds of sun-screen agents, 11 kinds of anticeptics and 10 kinds of vegetable oils was carried out using pet. ether:HOAc(88:12) as the solvent and succeeded in separating each component.6. It is possible to detect paraffin in animal and vegetable waxes and liquid paraffin which has been added to vegetable oils such as camellia oil as adulterant by TLC.(3). Column Chromatography1. Pilocarpin in hair-tonic was adsorbed from an absolute solvent system using Dowex 50WX-1 (H type) column and a yield of over 90% was obtained by eluting with N-HC1.2. Lecithin in pomade was adsorbed in CHC13 solution using a silica gel column and eluted with ethanol.(4). Potentiometric AnalysisSulfonic acid salts of commercial grade quinoline yellow WS etc., were made into the free form and then Potentiometric analysis was carried out with 0.02N-NaOH solution, by which it was possible to identify the number of sulfone groups.(5). Near-Infrared Absorption SpectraNear-infrared