Methods For Identification Of Enterobacteriaceae Research Articles

Rapid method for identification of Enterobacteriaceae in urinary tract infections.

Rapid method for identification of Enterobacteriaceae in urinary tract infections.

The scope and limitations of four methods for the rapid identification of Enterobacteriaceae in foods.

Four methods for the identification of Enterobacteriaceae in cooked frozen meat have been appraised. Although these methods could be modified to improve efficiency they could be clearly classified into two categories: (i) those that allow identification to genus level and which often cannot discriminate between two genera without the help of additional reactions; (ii) those that allow identification to species level and which only occasionally require additional reactions to differentiate one species from another. A different evaluation criterion was followed for each of these categories. In the first, as methods lead to final identification by means of data furnished by the manufacturer or via a number code which does not indicate probability, reactions were compared with those obtained by classical methods. In the second group, every number code admits three possibilities and indicates a probable biotype for each, thus reducing the risk of faulty interpretation on the part of the operator while also compensating for deficiencies of certain reactions. Evaluation was therefore based on the percentage of correctly identified cases per species. Efficiencies of the main biochemical reactions are also discussed.

Comparison of the MIC 2000 enteric media with API 20 E and conventional methods for identification of enterobacteriaceae

By using MIC 2000 Enteric Identification Media the identification of Enterobacteriaceae was evaluated. 421 strains of Enterobacteriaceae belonging to 23 taxa were identified by MIC 2000, and the conventional method API 20 E, respectively. The results obtained are almost identical, i.e. 96.2% and 96.7%. Besides, the results showed no statistical difference between the two miniaturized systems (p > 0.1). The MIC 2000 Enteric Media are capable of identifying the majority of Enterobacteriaceae with a reasonable degree of reliability. This system appears to be efficient and flexible. It is convenient to determine the minimal inhibitory concentration of the tested strain on the same microplate.

Clinical laboratory evaluation of the automicrobic system Enterobacteriaceae biochemical card.

The AutoMicrobic System Enterobacteriaceae Biochemical Card (AMS-EBC; Vitek Systems, Inc.) was evaluated in two clinical microbiology laboratories. A total of 502 consecutive clinical isolates representing members of the family Enterobacteriaceae were tested in parallel with the AMS-EBC, API 20E, and Enterotube II systems. Discrepancies between systems were resolved with the conventional methods of Edwards and Ewing (P. R. Edwards and W. H. Ewing [ed.], Identification of Enterobacteriaceae, 1972) and Ewing and Martin (W. H. Ewing and W. J. Martin, in Manual of Clinical Microbiology, 1974) AMS-EBC correctly identified 96.6% and incorrectly identified 3.4% of the isolates. When 12 or more isolates of a species were evaluated, Serratia marcescens, Proteus mirabilis, and Enterobacter cloacae posed the greatest challenge to the systems, with 92.6, 95.2, and 95.3%, respectively, being correctly identified. To confirm the accuracy of identification when all systems agreed, 93 randomly selected isolates were identified by conventional methods. The percent agreement was 100%. The reproducibility of triplicate determinations on 93 randomly selected isolates with the AMS-EBC was 99.6%. The AMS-EBC was found to be an easy, rapid, and accurate method for identification of Enterobacteriaceae.

Comparison of three methods for identification of Enterobacteriaceae

Comparison of three methods for identification of Enterobacteriaceae

Comparison of reagent-impregnated paper strips and conventional methods for identification of Enterobacteriaceae.

Comparison of reagent-impregnated paper strips and conventional methods for identification of Enterobacteriaceae.

Lysine Decarboxylase Activity as a Simple Test in Differentiation of Enterobacteriaceae

MANY efforts have been made to find simple methods for rapid identification of Enterobacteriaceae. Most of the tests are based on the ability of the bacteria to grow on selective media, or to ferment specific sugars, alcohol derivatives, etc. Such tests, in which bacteria are able to grow on selective media, are usually time-consuming, and positive results are recorded, at the earliest, only after 24 h.