Scientific Research Research Articles

ASSESSMENT OF THE OPPORTUNITIES OF MINERAL WATER RESOURSES FOR REGIONAL DEVELOPMENT – A CASE STUDY OF SOUTH-WESTERN PLANNING REGION, BULGARIA

The mineral water resources are the basis for the development for regions of balneology and SPA activities. The main goal of the present study is to analyze and evaluate the potential of mineral water resources and the state of balneo an d SPA tourism in the South-Western Planning Region of the country. Methods of scientific analysis and synthesis of the collected information database, and geographic information systems (GIS) were applied. The obtained results demonstrate that territory of the studied area has a serious potential of mineral water resources, which in case of sustainable and effective use, would contribute significantly to the development of tourism in the region.

Experimental Study on Corrosion of Stainless Steel in Low Temperature Multi effect Seawater Desalination

Starting from the corrosion mechanism, this paper analyzes the characteristics of various types of stainless steel and selects the best performance composite plate composite plate stainless steel. Analyze and select the most suitable corrosion detection method based on specific practical multi working conditions, discuss the interference factors that affect metal corrosion during experimental simulation, and the advantages of newly developed sheet metal. The new development of composite board panels, with the substrate and composite materials applying their respective capabilities for MED, will bring breakthrough progress to the scientific research and engineering application of composite boards.

Traditional Chinese Medicine's Spleen Strengthening and Intestinal Microecology

The "spleen" in traditional Chinese medicine is the "acquired foundation" and the "source of qi and blood biochemistry". Strengthening the "spleen" is one of the important treatment methods in traditional Chinese medicine; The gut microbiota has been a hot topic in modern scientific research in recent years, and its nutritional and immune functions correspond to some of the functions of the spleen in traditional Chinese medicine. In terms of philosophy, traditional Chinese medicine emphasizes the balance between the spleen and the gut microbiota, emphasizing the importance of supporting the right and eliminating evil, preventing diseases before they occur, seeking the root cause of treatment, and adapting measures to the three causes; Clinically, the treatment of obesity, constipation, diarrhea and other diseases by invigorating the spleen and the treatment mechanism of acupuncture and moxibustion, chiropractic and invigorating the spleen are all related to microecology. This article combines relevant literature on traditional Chinese medicine's "spleen" and "spleen health" to explore the treatment concept, clinical application, and correlation with intestinal microbiota of traditional Chinese medicine's "spleen health". It also elaborates on the latest literature, aiming to exchange ideas with peers and promote research on the microbiota of traditional Chinese medicine's "spleen".

A Biopsychosocial Model for Understanding Training Load, Fatigue, and Musculoskeletal Sport Injury in University Athletes: A Scoping Review.

McClean, ZJ, Pasanen, K, Lun, V, Charest, J, Herzog, W, Werthner, P, Black, A, Vleuten, RV, Lacoste, E, and Jordan, MJ. A biopsychosocial model for understanding training load, fatigue, and musculoskeletal sport injury in university athletes: A scoping review. J Strength Cond Res 38(6): 1177-1188, 2024-The impact of musculoskeletal (MSK) injury on athlete health and performance has been studied extensively in youth sport and elite sport. Current research examining the relationship between training load, injury, and fatigue in university athletes is sparse. Furthermore, a range of contextual factors that influence the training load-fatigue-injury relationship exist, necessitating an integrative biopsychosocial model to address primary and secondary injury prevention research. The objectives of this review were (a) to review the scientific literature examining the relationship between training load, fatigue, and MSK injury in university athletes and (b) to use this review in conjunction with a transdisciplinary research team to identify biopsychosocial factors that influence MSK injury and develop an updated, holistic biopsychosocial model to inform injury prevention research and practice in university sport. Ten articles were identified for inclusion in this review. Key findings were an absence of injury surveillance methodology and contextual factors that can influence the training load-fatigue-MSK injury relationship. We highlight the inclusion of academic load, social load, and mental health load as key variables contributing to a multifactorial, gendered environmental, scientific inquiry on sport injury and reinjury in university sport. An integrative biopsychosocial model for MSK injury in university sport is presented that can be used to study the biological, psychological, and social factors that modulate injury and reinjury risk in university athletes. Finally, we provide an example of how causal inference can be used to maximize the utility of longitudinally collected observational data that is characteristic of sport performance research in university sport.

Reframing the experience of childbirth: Black doula communication strategies and client responses during delivery hospitalization

Doulas, or birth coaches, are said to be “changing the world, one birth at a time.” Black doulas have been suggested to mitigate against lack of representation in obstetric care, limited identity acknowledgement and accommodation, and obstetric racism. However, scientific inquiry into the specific communication strategies and messages used by Black doulas to advocate for clients was non-existent in extant literature. Guided by the Agency-Identity Model, we analyzed 20 diary-interviews of nine Black doulas who recently served Black clients. Specifically, we explored Black doulas’ communication strategies and whether these strategies had an impact on client agency. We found that Black doulas prepare their clients for patient-provider interactions, including conversations about certain medical treatments and procedures and the risks for Black women and birthing people, the importance of informed consent, how to be heard, and how to resist neglect or abuse. We found that, in turn, most Black clients were able to enact agentic responses. We describe the specific doula messages, and contextualize our findings, considering how these collective interpersonal communication strategies of Black doulas, and their clients’ agentic transformations, may index a sociopolitical movement to reframe the experience of childbirth in America.

Changing pasture management practices on the Greek island of Samothraki: Obstacles and opportunities

ContextOn the Greek island of Samothraki, decades of overgrazing by the large domestic population of small ruminants accelerated soil degradation and surface erosion, with direct consequences for ecosystem functioning and the delivery of ecosystem services. ObjectiveThis manuscript reports on a 5-year research project to achieve more sustainable pasture management practices among small ruminant farmers on the island, through the introduction of Sown Biodiverse Pastures (SBP). This practice, based on sowing a seed mixture of legumes and grasses that increase pasture productivity, has proven to be a successful tool to overcome degradation of ruminant pastures in Portugal. MethodsLocal small ruminant farmers on Samothraki, a difficult group when it comes to the acceptance of new practices and ideas, were engaged in the transdisciplinary research process, and trained in appropriate management practices of SBP. This led to the adoption and implementation of SBP by nine farmers on 13 parcels. Results and conclusionsQuantitative data on species composition and productivity shows that the performance and persistence of SBP on Samothraki is favourable, if soil preparation and sowing is practiced as recommended, and relevant management practices are adopted. Comparing with conventional agricultural practices, i.e. annual crop – fallow rotation, productivity in SBP outperformed the forage quantity in fallow land, while yield in the annual crop was approximately equal as compared to SBP. The increasing forage capacity of SBP instigates several environmental and economic benefits, such as a reduction of grazing pressure in vulnerable areas, and less expenditure on supplementary feed requirements. However, lack of will and trust in these new practices and seed mixtures among local farmers resulted in discontinuation in almost half of the parcels. The adoption of new management practices by the farming community on Samothraki required continuous efforts, and the short-term framework of the research project did not favour long-term success. SignificanceIt is recommended for any programs aiming at changing farming practices to engage with local stakeholders, especially farmers, and to closely collaborate with local institutional partners who can carry the work forward after scientific researchers have left.

A Complete Sojourn of Current Trends in Gastro-retentive Drug Delivery System: Recent Advances and Patent Survey.

The current work aims to provide a complete sojourn on gastro-retentive drug delivery system (GRDDS) along with formulation methods, polymer selection, and in vitro/ in vivo challenges with finished dosage forms. Ideally, a biopharmaceutical-hindered drug has a rapid clearance and erratic bioavailability due to its low aqueous solubility and permeability. Additionally, it also suffers from high first-pass metabolism and pre-systemic gut wall clearance. Gastro-retentive drug delivery systems have become an emerging technology where newer methodologies and scientific approaches have been used to provide the controlled release of drugs and provide a protective mechanism in the stomach. By the virtue of utilizing GRDDS as a dosage form, these formulations increase Gastroretention time (GRT) which prolongs the controlled release of the drug in the dosage form. GRDDS contribute to increased drug bioavailability and targeting at a site of action, which enhances therapeutic action and offers significant patient compliance. Furthermore, the present work also highlighted the critical role of polymers in favoring drug retention across GIT with the mechanism of gastro-retention and recommended concentration ranges. The emerging technology is also highlighted by the approved drug products and patented formulations in the recent decade which is depicted in a justified manner. GRDDS formulations have demonstrated clinical efficacy, which is supported by a compilation of patents for cutting-edge innovations in dosage forms that can be held in the stomach for an extended period of time.

The Influence of Shakarim Kudaiberdiev’s Work on the Economic, Cultural, and Spiritual Development of Kazakhstan in the Late 19th Century and Early 20th Century

The significance and relevance of the issues are predetermined by the fact, that, by the time of Shakarim Kudaiberdiev’s activity, the political and social situation of Kazakhstan in the early 20th century was on the verge of collapse. The main factor that influenced this situation was the policy of spreading illiteracy, people alienated from religion language, and writing. From the late 19th and early 20th centuries, Kazakhstan suffered from the colonial policies of the Russian Empire. Currently Kudaiberdiev writes a work “Catechism of Muslims”. The main purpose of the study is to analyze the consequences of the creative work on the economic and spiritual and cultural development of Kazakhstan. During the analysis, several scientific sources and materials were analyzed, and the following scientific methods were used: structural-functional and dialectical methods, the method of synthesis, logical and comparative analysis, and the method of generalization. During the research, it was proved that in the works of Shakarim Kudaiberdiev the calls to fight against the policy of tsarist Russia and to revive their language, customs, religion, and history were present. The main result of this research is proof that the works of Shakarim Kudaiberdiev had a tremendous impact on the social status, culture, religion, and language of the Kazakh people. His works acted in opposition to Russian policy and encouraged people who were tired of colonial oppression. The research on this issue is actively developed, therefore this work is of practical value for the definition of the role of Shakarim’s creativity during the economic, cultural, social, and political development of Kazakhstan at the end of 19 – the beginning of 20 century.

Analysis of causes and factors of the spread of takfirism in Kazakhstan

The purpose of this research is to analyse the emergence of takfirism, its spread and its evolution in contemporary Kazakh society. The methodological foundation of the study was provided by general theoretical methods of scientific cognition, and various methods of collecting and systematising information. Among the general scientific methods are the method of theoretical analysis; the historical method of comparing individual religious movements of different periods in Kazakhstan, and the specific scientific and philosophical-religious methods of research. The results have suggested that unfounded takfirism poses a threat to the sustainability of diverse communities in Kazakhstan. The religious, ideological and theological preconditions for takfirism in Kazakhstan were analysed. Noted the diversity of approaches by Muslim scholars to understanding and interpreting the ayats of the Qur'an. Presented aspects of distortion in interpreting the essence of Shariah texts. Noted that the influence of political and social factors is significant. Focuses on some of the socio- psychological aspects of the development of radical attitudes among young people. The qualities inherent in young people that enable them to resist external non-ideological attacks are identified. Illiteracy, poor quality of education and the low culture of some people in Muslim countries are among the key elements. It was noted that the main factor in takfirism in Kazakhstan is the influence of the Salafi-Wahhabi movement. The practical significance of the study is that its content is of interest in developing the worldview of contemporary young people in the Republic of Kazakhstan. The results can be used as a teaching and practical material for students and researchers on contemporary history and religion in Kazakhstan.

Legal regulation of confessional relations in Kazakhstan

Multinationalism and the presence of many different religious groups in the country can be causes of inter-ethnic and inter-confessional conflicts. A tool to help avoid them is the implementation of an effective legislative mechanism. The research aims to investigate the legal foundations of confessional relations in Kazakhstan, including constitutional norms, norms of laws and bylaws, and the provisions of international acts in the sphere of relations between the state and religion. The system of methods of scientific knowledge of different levels – from the most general methods to special methods of legal science – was used to implement the goal. The study, the model of state- confessional relations of the Republic of Kazakhstan, its basic principles, as well as the existing legal support of this model were analysed and conducted. The authors concluded that the legislation of the Republic of Kazakhstan regulating confessional relations partially corresponds to international normative legal instruments regulating freedom of religion. In addition, the study showed that the model of state-confessional relations in force in the Republic of Kazakhstan contributes to the observance of citizens’ rights to freedom of religion, the prevention of inter-confessional conflicts among religious groups within the country, and the establishment of peace. The practical significance of the results makes it possible to assess the effectiveness of the current mechanism of legislative regulation of confessional relations and to identify the main problems and ways to resolve them.

A thorough assessment of various forest management planning initiatives and development of improvement strategies towards an ecosystem-based planning

Management planning for forests in Turkey has undergone gradual evolution over the last century. Developing and implementing a comprehensive management planning framework present significant challenges. This paper evaluates the effectiveness of various forest management planning approaches implemented across the country over different time periods, with overarching planning principles. It formulates a robust planning framework and proposes improvements grounded in scientific advancements and international standards. The assessment indicated that the management plans were developed using reputable scientific methods and principles aimed at ensuring the sustainable management of forest resources with certain strengths and opportunities presented by SWOT analysis. All management plans shared a common planning concept primarily focused on maximizing wood production through the area-control harvest scheduling method (except for continuous cover forest, which employed the single tree selection method). Each management plan established its unique vision, targets, policies, objectives, and planning guidelines. Forest inventory data were gathered through a combination of ground surveys and remotely sensed data to characterize and stratify the landscape. A robust in-house management authority and governance system with appropriate technical capacity and guidelines were developed and implemented, fostering a sound common working culture and tools. However, some notable drawbacks were identified, including political pressure, biomass/carbon accounting, growth-yield modelling, economic analysis, limited characterization of the full range of ecosystem services, risk and uncertainty analysis, food security and, particularly, long-term sustainability and scenario analysis with the appropriate decision-making tools and methods. Despite a few strengths, these limitations may raise concerns about the far-sighted design and application, potentially jeopardizing the sustainable management of forest ecosystems. Proposed improvement strategies for an efficient forest ecosystem management planning system include characterization of ecosystem services, modelling their productivity, scenario analysis with a decision support system, stakeholder involvement, balancing utilization and conservation targets, conducting risk and uncertainty analysis and economic analysis of management actions.

A CURE Lab in Introductory Biology at a Regional Comprehensive University Negatively Impacts Student Success in the Associated Lecture Course Among Students from Groups Underrepresented in Science.

Course-based undergraduate research experiences (CUREs) have been proposed as a mechanism to democratize access to the benefits of apprentice-style scientific research to a broader diversity of students, promoting inclusivity and increasing student success and retention. As we evaluate CUREs, it is essential to explore their effectiveness within the environments of regional comprehensive universities and community colleges, because they are important access points for a wide variety of students. It is also important to address the potential influence of volunteer bias, where students can opt to enroll in either the CURE or a traditional lab, on the outcomes of CUREs. We evaluated a CURE at a regional comprehensive university under conditions both with and without volunteer bias. We find that nonvolunteer students report a lower sense of discovery and relevance of the CURE compared with students who volunteered for the course. Importantly, we also find that our replacement of the traditional lab class with a CURE resulted in lower scores on exams in the associated lecture course among students who are both BIPOC and Pell eligible. We call for additional research on the effects of CUREs at nonresearch-intensive institutions and without volunteer bias, to better understand the impact of these classes.

Magnetically induced asymmetry and fuel-propulsion of lipid vesicles containing Fe3O4 micromotors

Artificial micro/nanomotors possess the capability to navigate intricate environments in accordance with a premeditated blueprint. Hybrid-propelled micromotors may present an efficacious approach in addressing scientific research quandaries. In this context, ferric oxide magnetic nanoparticles (Fe3O4 MNPs) are chosen as catalysts to be encapsulated within lipid vesicles (LVs) due to their superior quality and cost-effectiveness. The external magnetic field induces the aggregation of Fe3O4 MNPs within the LVs, resulting in the formation of an asymmetrical structure that promotes the catalytic reaction of hydrogen peroxide, producing oxygen to propel the movement of Fe3O4@LVs micromotors. The magnetic-fuel dual drive enables the Fe3O4@LVs micromotor to move 1.3 times faster than the LVs alone. Additionally, the magnetic field offers the advantage of controlling the direction of motor movement and facilitating retrieval for practical applications. This dual drive micromotor exhibits exceptional stability and reusability in aqueous solutions, underscoring its immense potential for future applications.

An Exploration of Organoid Technology: Present Advancements, Applications, and Obstacles.

Organoids are in vitro models that exhibit a three-dimensional structure and effectively replicate the structural and physiological features of human organs. This work examines the potential applications of organoid technology, as well as the challenges and future directions associated with its implementation. The manuscript was put together by conducting a comprehensive literature review, which involved an in-depth evaluation of globally renowned scientific research databases. The field of organoids has generated significant attention due to its potential applications in tissue development and disease modelling, as well as its implications for personalised medicine, drug screening, and cell-based therapies. The utilisation of organoids has proven to be effective in the examination of various conditions, encompassing genetic disorders, cancer, neurodevelopmental disorders and infectious diseases. The exploration of the wider uses of organoids is still in its early phases. Research shall be conducted to integrate 3D organoid systems as alternatives for current models, potentially improving both fundamental and clinical studies in the future.

Challenges and tasks of the philosophy of physical culture and sports, sports science in modern socio-political conditions

The scientific problem lies in the discrepancy between the self-reflective Western and Eastern traditions of the philosophy of sport and the very real sports process and sports science, and, by and large, the classical understanding of the scope and content of the concept of philosophy as such. The relevance of the study lies in the need to eliminate or «remove» this discrepancy, which at three levels openly complicates the development of the philosophy of sport: firstly, as a philosophy in the classical sense of the word; secondly, as a scientific philosophy; and thirdly, as a scientific philosophy of sports and sports science, and not existential, irrational, hermeneutic and other ideas and experiences about philosophizing thoughts about something vaguely reminiscent of them. The purpose of the study is to construct and briefly justify a classification of the scientific philosophy of sports and sports science from the standpoint of classical philosophy and a dialectical-materialistic worldview, as well as to indicate a short list of its main tasks in the current situation of aggravated military-political confrontation between Russia, East and West. The methodology and organization of the study are based on a comprehensive historical, philosophical and scientific theoretical and comparative analysis, including general scientific operations of grouping, systematization, abstraction, idealization and logical operations of defining and dividing concepts, induction, deduction, analogy, determining truth, proof and refutation, constructing a hypothesis. Research results and conclusions. Gradually but steadily growing since the beginning of the 21st century, global changes in the modern socio-political picture of the world are increasingly clearly and rapidly entailing equally significant changes in education and science (including specialized ones). This article presents a system for constructing the philosophy of sport and sports science, which provides a solution to the full range of modern problems of philosophical analysis and scientific research of sport as: • social phenomenon and institution; • cultural and civilizational mechanism of social evolution of humanity; • a mechanism for preserving and improving a person in his inherent parameters of a social and psychophysiological being; • an outpost for preventing the processes of human deanthropolization. It is easy to notice that this system of constructing the philosophy of sport and sports science in many aspects and in terms of system-forming factors differs significantly from its predecessors, at the same time serving as the basis for their scientific and philosophical criticism — criticism that substantiates the urgent need for a radical restructuring of the entire edifice of the philosophy of physical culture and sports, based on from the modern socio-political challenges addressed to her.

The scientific legacy of professor Y.V. Krykavskyi: development of logistics theory in Ukraine and future perspectives

The article presents the first attempt at scientometric analysis of the scientific legacy of the prominent Ukrainian scholar, Doctor of Economic Sciences, Professor Yevhen Vasylovych Krykavskyi. The main stages of his scientific work and its impact on the development of the general theory and practice of logistic science in Ukraine are identified. It is demonstrated that Y.V. Krykavskyi himself formulated the theoretical foundations of logistics as a separate direction of scientific research in the field of economic sciences, established the basic conceptual framework, founded a scientific school of marketing and logistics, and trained a large number of candidates and doctors of sciences who continue and deepen the theoretical developments of the great scientist, as well as expand the spheres of practical application of logistic concepts, tools, and methods, and supply chain management in new economic conditions.

Technology Prospection of Ora-pro-Nóbis (Pereskia aculeata Mill.): A Non-conventional Food Plant.

The ora-pro-nóbis (Pereskia aculeata Mill.) is a plant from Brazilian biodiversity used for food and medicinal purposes. It has ample technological potential, however, it is still underutilized, being classified as a Non-Conventional Food Plant (PANC). Prospective studies in intellectual property banks make it possible to expand perspectives for scientific research, enhancing the generation of new products. Evaluate the patents of products containing Pereskia aculeata Mill. for the areas of food and health in intellectual property databases. The study was conducted through structured prospective investigation (collection, processing and analysis) in 4 patent databases: National Institute of Intellectual Property (INPI) - Brazil, United States Patent and Trademark Office, World Trade Organization Intellectual Property (WIPO) and Espacenet. The evaluation showed a reduced number of registered patents. In general, 8 patent applications were examined, of which 7 are directly associated with the species (and its derivatives) and 1 is related to a device specially designed for harvesting leaves/fruits and removing thorns. The focus of the patents was the use of the species in the food, pharmaceutical and biotechnological areas, with emphasis on the use of the leaves in the extraction of mucilage and proteins. This study showed that Pereskia aculeata Mill. is a technologically promising plant, because of its nutritional and medicinal composition, and it is important to encourage innovation and the development of new products with the species.

Genomic and Metagenomic Insights into the Distribution of Nicotine - degrading Enzymes in Human Microbiota

Introduction: Nicotine degradation is a new strategy to block nicotine-induced pathology. The potential of human microbiota to degrade nicotine has not been explored. Aims: This study aimed to uncover the genomic potentials of human microbiota to degrade nicotine. Method: To address this issue, we performed a systematic annotation of Nicotine-Degrading Enzymes (NDEs) from genomes and metagenomes of human microbiota. A total of 26,295 genomes and 1,596 metagenomes for human microbiota were downloaded from public databases and five types of NDEs were annotated with a custom pipeline. We found 959 NdhB, 785 NdhL, 987 NicX, three NicA1, and three NicA2 homologs. Results: Genomic classification revealed that six phylum-level taxa, including Proteobacteria, Firmicutes, Firmicutes_A, Bacteroidota, Actinobacteriota, and Chloroflexota, can produce NDEs, with Proteobacteria encoding all five types of NDEs studied. Analysis of NicX prevalence revealed differences among body sites. NicX homologs were found in gut and oral samples with a high prevalence but not found in lung samples. NicX was found in samples from both smokers and non-smokers, though the prevalence might be different. Conclusion: This study represents the first systematic investigation of NDEs from the human microbiota, providing new insights into the physiology and ecological functions of human microbiota and shedding new light on the development of nicotine-degrading probiotics for the treatment of smoking-related diseases.

A systematic investigation of grooving and rolling abrasive mechanisms using surface topography

Rolling and grooving are typical phenomena observed in ball cratering tests. Despite extensive research, their impact on the wear rate remains a subject of debate. Besides, the identification of rolling and grooving is predominantly performed with qualitative analysis. Therefore, this investigation introduced a systematic methodology for the assessment of the worn surface topography. Ball cratering tests were performed with increasing load to induce the transition between rolling and grooving mechanisms. Several roughness metrics were analysed, among which the surface aspect ratio (Str) was found the most effective parameter for identifying the transition between rolling and grooving. Additionally, the transition was found to occur in a range of load conditions. Moreover, a segmented analysis was implemented and further revealed a propensity for grooving to manifest towards the centre and trailing edge of the wear crater. Finally, it was found no discernible difference in the wear rate between rolling and grooving conditions, and different hypotheses were discussed to explain this behaviour. The superposition of mechanisms and the occurrence of grooving by rolling was found plausible explanations for the similar wear rate. As consequence, an improved terminology for rolling and grooving was proposed. The term rolling refers to the micro abrasive mechanism, while grooving designates the resulting failure mode.

Evaluation of antiplasmodial potential of Cissampelos pareira L.: Characterization and quantification of isoquinoline alkaloids responsible for the biological activity

Globally, based on traditional knowledge, different parts of Cissampelos pareira plant are being used individually or in combination in various forms to manage malaria. However, the scientific investigation for validating the most effective part of this plant against malaria parasite has not been done. Thus, the study aims to explore the antiplasmodial potential of different parts of the plant against malaria parasite by bio-assay guided isolation of compounds from different extracts and fractions. Antiplasmodial activities of the whole plant parent extract and its fractions along with decoctions (roots, stem, leaves and whole plant) and isolated molecules were evaluated against chloroquine-sensitive Pf3D7 and chloroquine-resistant PfINDO strains of Plasmodium falciparum. A UPLC-DAD based method was developed to quantify the marker compounds in all samples by utilizing isolated molecules as analytical standards. The chloroform fraction of the whole plant was found to be the most potent with IC50 (µg/mL) of 0.79 (Pf3D7) and 2.26 (PfINDO) followed by root decoction with IC50 (µg/mL) 10.22 (Pf3D7) and 7.7 (PfINDO). Further, phytochemical analysis of active chloroform fraction led to the isolation of two potent bisbenzylisoquinoline compounds, namely O,O-dimethylcurine (1) [IC50 (µM) 1.46 (Pf3D7) and 0.51 (PfINDO)], and curine (2) [IC50 (µM) 1.46 (Pf3D7) and 0.51 (PfINDO)]. Analysis of developed UPLC-DAD method showed the highest quantities of curine (2) (∼107 mg/g) and O,O-dimethylcurine (1) (∼15 mg/g) in chloroform fraction which might be responsible for its potent activity. This study showed that the root decoction was more effective than decoctions of each of the other parts of the plant and the whole plant hydroalcoholic extract.