The article investigates some issues of complex systems analysis and synthesis that contain local, discrete sources of temperature fields. The emphasis of the author's research lies in the calculation and optimization of the parameters of the laser action on the embryo. The biotechnological process is described by the boundary value problem of a non-stationary, multidimensional differential equation of thermal conductivity which satisfies the boundary conditions of heat flux, beginning and end of the laser action process. The author proposes an algorithm for calculation and optimization of the control parameters of laser action on multilayer microbiological material. The object of the study is the embryo under the action of a laser beam for fission. As a first approximation, at the stage of calculation and optimization of the parameters of the biotechnological process, the embryo is considered as a homogeneous body. The values of thermophysical characteristics are calculated by the method of expert evaluation of the parameters of the emitters. The correctness of the boundary value problem of the process of laser action on the embryo is proved by the author using the method of pseudodifferential operators. Seeking the solution of the boundary value problem in the form of the series, the analytical function of the temperature field distribution is obtained using the Fourier method of the separated variables in the article. Using the method of indeterminate coefficients, the author found the temperature of the laser action on the embryo. Using the approximate gradient method of finding local extrema and the method of directed search of local extrema, it is possible to obtain rational values of optimized parameters of the biotechnological process. The author outlines possible approaches for optimization of technical parameters of laser emitters. In his opinion, the results of research can be considered fundamental for the calculation and optimization of the parameters of the laser action on the embryo, taking into account the multilayer structure of the latter.