High-resolution Imaging Method Research Articles

ECCENTRIC: A fast and unrestrained approach for high-resolution in vivo metabolic imaging at ultra-high field MR

Abstract A novel method for fast and high-resolution metabolic imaging, called ECcentric Circle ENcoding TRajectorIes for Compressed sensing (ECCENTRIC), has been developed at 7 Tesla MRI. ECCENTRIC is a non-Cartesian spatial-spectral encoding method designed to accelerate magnetic resonance spectroscopic imaging (MRSI) with high signal-to-noise at ultra-high field. The approach provides flexible and random sampling of the Fourier space without temporal interleaving to improve spatial response function and spectral quality. ECCENTRIC enables the implementation of spatial-spectral MRSI with reduced gradient amplitudes and slew-rates, thereby mitigating electrical, mechanical, and thermal stress of the scanner hardware. Moreover, it exhibits robustness against timing imperfections and eddy-current delay. Combined with a model-based low-rank reconstruction, this approach enables simultaneous imaging of up to 14 metabolites over the whole brain at 2–3 mm isotropic resolution in 4–10 min. MRSI ECCENTRIC was performed on four healthy volunteers, yielding high-resolution spatial mappings of neurochemical profiles within the human brain. This innovative tool introduces a novel approach to neuroscience, providing new insights into the exploration of brain activity and physiology.

High-resolution acoustic imaging method based on block sparsity constraint

Acoustic imaging method is a critical task in various applications since it can locate the sound sources. However, the resolution of the method becomes low at low frequencies. This paper proposes a novel method to realize high-resolution acoustic imaging based on block sparsity constraint. By dividing the focusing area into blocks, the block sparse property of sound sources can be utilized to improve the resolution of the result. In this study, a block orthogonal matching pursuit method is introduced to deal with source localization problem with block sparsity constraint. To evaluate the performance of the proposed method, it is tested through numerical simulations and an experiment. The results of simulations prove that the proposed acoustic imaging method based on block sparsity constraint can localize sound sources accurately and performs better than that based on sparsity constraint. The results of the experiment further validate these findings.

A High-Resolution and Robust Microwave Correlation Imaging Method Based on URRF Using MC-AAMPE Algorithm

This manuscript presents a novel framework for high-resolution and robust microwave correlation imaging. In order to generate a more diverse random radiation field distribution, the unified random radiation field (URRF) model is proposed. The URRF model can accurately characterize the joint random modulation in the signals’ phase, amplitude, and frequency. Furthermore, we build a parametric imaging model based on URRF which clearly describes the relationship between the image to be reconstructed and the signals by the URRF model. By using this imaging model, the reconstruction of an image is converted into solving a multi-parameter optimization problem with multiple constraints. To solve this optimization problem with high efficiency and accuracy, the model-constrained adaptive alternating multiple parameter estimation (MC-AAMPE) algorithm is proposed. This algorithm decomposes the high-dimensional multi-parameter optimization problem into several sub-optimization problems. The renewing solutions to these sub-optimization problems make the multi-parameter optimization converge to the image of the target and the parameters of clutter and noise, which are all unknown before the solution. In comparison with the existing methods, the proposed scheme generates images with higher resolution and is more robust under noise conditions. Extensive simulation experiments confirmed the effectiveness and robustness of the proposed method.

High Refractive Index Imaging Buffer for Dual-Color 3D SMLM Imaging of Thick Samples.

The current limitations of single-molecule localization microscopy (SMLM) in deep tissue imaging, primarily due to depth-dependent aberrations caused by refractive index (RI) mismatch, present a significant challenge in achieving high-resolution images at greater depths. To extend the imaging depth, we optimized the imaging buffer of SMLM with the RI matched to that of the objective immersion medium and systematically evaluated five different RI-matched buffers, focusing on their impact on the blinking behavior of red-absorbing dyes and the quality of reconstructed super-resolution images. Particularly, we found that clear unobstructed brain imaging cocktails-based imaging buffer could match the RI of oil and was able to clear the tissue samples. With the help of the RI-matched imaging buffers, we showed high-quality dual-color 3D SMLM images with imaging depths ranging from a few micrometers to tens of micrometers in both cultured cells and sectioned tissue samples. This advancement offers a practical and accessible method for high-resolution imaging at greater depths without the need for specialized optical equipment or expertise.

Quantitative Optimization of Handheld Probe External Pressure on Dermatological Microvasculature Using Optical Coherence Tomography-Based Angiography.

Optical Coherence Tomography (OCT)-based angiography (OCTA) is a high-resolution, high-speed, and non-invasive imaging method that can provide vascular mapping of subcutaneous tissue up to approximately 2 mm. In dermatology applications of OCTA, handheld probes are always designed with a piece of transparent but solid contact window placed at the end of the probe to directly contact the skin for achieving better focusing between the light source and the tissue, reducing noise caused by minor movements. The pressure between the contact window and the skin is usually uncontrollable, and high external pressure affects the quality of microvascular imaging by compressing the vessels and obstructing the underlying blood flow. Therefore, it is necessary to determine a pressure range to ensure that the vessels can be fully imaged in high-quality images. In this paper, two pressure sensors were added to the existing handheld OCT probe, and the imaging probe was fixed to a metal stand and adjusted vertically to change the pressure between the probe and the tested skin site, a gradient of roughly 4 kPa (with 1-2 kPa error) increase was applied in each experiment, and the impact of pressure to the vessel was calculated. The experiment involved a total of five subjects, three areas of which were scanned (palm, back of the hand, and forearm). The vessel density was calculated to evaluate the impact of external pressure on angiography. In addition, PSNR was calculated to ensure that the quality of different tests was at a similar level. The angiography showed the highest density (about 10%) when the pressure between the contact window on the probe and the test area was between 3 and 5 kPa. As the pressure increased, the vascular density decreased, and the rate of decrease varied in different test areas. After fitting all the data points according to the different sites, the slope of the fitted line, i.e., the rate of decrease in density per unit value of pressure, was found to be 4.05% at the palm site, 6.93% at the back of the hand, and 4.55% at the forearm site. This experiment demonstrates that the pressure between the skin and contact window is a significant parameter that cannot be ignored. It is recommended that in future OCTA data collection processes and probe designs, the impact of pressure on the experiment be considered.

Truncated Newton full waveform inversion method for the human brain imaging

Abstract It has been shown that full-waveform inversion (FWI) method can be a competitive alternative for medical imaging problems. It offers high-resolution results while delivering the advantages of being fast, safe, portable, and affordable, compared to magnetic resonance imaging (MRI) and x-ray computed tomography. However, to the best of our knowledge, FWI applications in medical imaging only use the first-order derivative information. In this case, the high parameter contrasts between different tissues of human body and multi-scatterings problems may lead FWI to local minima. Thus, we present a competitive truncated Newton method for high-resolution imaging of the human brain. This truncated Newton method, based on the efficient linear solver MINRES-QLP, can make full use of multiple scattering wavefield information. Compared with the truncated Newton method based on conjugate gradient, the MINRES-QLP iterative method presents various advantages when solving linear systems, such as the capacity to handle both non-singular and singular systems, less computational cost, and efficiency even for ill-conditioned systems. Numerical experiments for imaging the brain with and without the skull are conducted. Numerical results indicate that, compared with the truncated Newton method based on conjugate gradient, the truncated Newton method based on MINRES-QLP exhibits computational efficiency while maintaining the same level of accuracy.

Structures of Liquid-Liquid Interfaces in Partially Miscible Systems Revealed by Soft X-ray Imaging.

The properties of liquid-liquid interfaces are intricately linked to its structure, with a particular focus on the concentration distribution within the interface. To obtain precise information regarding the concentration distribution, we have developed a high-resolution soft X-ray imaging method for liquid-liquid interfaces. This work focused on representative partially miscible systems, analyzing the interfacial concentration distribution profiles of water-alkanols under both steady-state and dynamic processes, and obtaining the diffusion coefficients of different water concentrations in alkanols. Significant disparities in concentration distributions and the concentration-related diffusion coefficients were observed despite comparable diffusion distances within the same system across different states. Meanwhile, it was found that alkanols exhibit adsorption phenomena at the interface. This newfound knowledge serves as a crucial stepping stone toward a deeper understanding of partially miscible systems. Our study opens a way to explore liquid-liquid interface information with high-resolution.

Vibrational imaging of metabolites for improved microbial cell strains.

Biomanufacturing utilizes modified microbial systems to sustainably produce commercially important biomolecules for use in agricultural, energy, food, material, and pharmaceutical industries. However, technological challenges related to non-destructive and high-throughput metabolite screening need to be addressed to fully unlock the potential of synthetic biology and sustainable biomanufacturing. This perspective outlines current analytical screening tools used in industrial cell strain development programs and introduces label-free vibrational spectro-microscopy as an alternative contrast mechanism. We provide an overview of the analytical instrumentation currently used in the "test" portion of the design, build, test, and learn cycle of synthetic biology. We then highlight recent progress in Raman scattering and infrared absorption imaging techniques, which have enabled improved molecular specificity and sensitivity. Recent developments in high-resolution chemical imaging methods allow for greater throughput without compromising the image contrast. We provide a roadmap of future work needed to support integration with microfluidics for rapid screening at the single-cell level. Quantifying the net expression of metabolites allows for the identification of cells with metabolic pathways that result in increased biomolecule production, which is essential for improving the yield and reducing the cost of industrial biomanufacturing. Technological advancements in vibrational microscopy instrumentation will greatly benefit biofoundries as a complementary approach for non-destructive cell screening.

Practical considerations for birefringence microscopy of myelin structure: Microscope design and tissue processing for effective imaging

Abstract Despite the interest in studying and quantifying the structural integrity of myelin in postmortem brain tissue, current methods for high-resolution imaging of myelin with optical microscopy are not sufficient. While imaging methods must have adequate resolution and sensitivity to detect microstructural alterations to myelin that are relevant in aging and neurodegenerative disease, an equally critical aspect is to minimize myelin damage that is induced during tissue processing steps. Birefringence microscopy (BRM) is a powerful technique that leverages the structural anisotropy of myelin to provide detailed, label-free images of myelin at any diffraction-limited optical resolution, while maintaining a simple and low-cost setup. Building on our previous work, we have developed a new BRM system and image processing pipeline that enable efficient, high-throughput imaging of myelin structure at multiple scales. Here, we utilize this system to systematically assess the damage to myelin that is induced by several common tissue processing steps in brain sections from the rhesus monkey. Images taken of the same myelinated axons, before and after each tissue processing step, provide direct evidence that mishandling of tissue during sample preparation can cause significant structural alterations to myelin. First, we report on key advancements to our BRM system, imaging procedure, and image processing pipeline, which provide significant increases to the speed and efficiency of BRM. These include integrating fast piezoelectric rotational stages, minimizing the number of images required (to three images) for determining birefringence parameter maps, and implementing an analytical solution for directly determining birefringence parameter maps. Second, using this BRM system, we demonstrate that effective myelin imaging requires (1) the avoidance of prolonged drying or dehydration of tissue, (2) the selection of the optimal mounting medium (85% glycerol), (3) the avoidance of tissue permeabilization with detergents (i.e., Triton X-100 and Saponin), and (4) the selection of a suitable tissue-section thickness (15, 30 and 60 µm) based on the region of interest. In addition to serving as a guide for new users interested in imaging myelin, these basic experiments in sample preparation highlight that BRM is very sensitive to changes in the underlying lipid structure of myelin and suggest that optimized BRM can enable new studies of myelin breakdown in disease. In this work, we show that BRM is a leading method for detailed imaging and characterization of myelin, and we provide direct evidence that the structure of myelin is highly sensitive to damage during inadequate preparation of brain tissue for imaging, which has previously not been properly characterized for birefringence imaging of myelin. For the most effective, high-resolution imaging of myelin structure, tissue processing should be kept to a minimum, with sections prevented from dehydration and mounted in 85% glycerol. With proper preservation of myelin structure, BRM provides exquisitely detailed images that facilitate the assessment of myelin pathology associated with injury or disease.

Mouse brain elastography changes with sleep/wake cycles, aging, and Alzheimer's disease

Understanding the physiological processes in aging and how neurodegenerative disorders affect cognitive function is a high priority for advancing human health. One specific area of recently enabled research is the in vivo biomechanical state of the brain. This study utilized reverberant optical coherence elastography, a high-resolution elasticity imaging method, to investigate stiffness changes during the sleep/wake cycle, aging, and Alzheimer's disease in murine models. Four-dimensional scans of 44 wildtype mice, 13 mice with deletion of aquaporin-4 water channel, and 12 mice with Alzheimer-related pathology (APP/PS1) demonstrated that (1) cortical tissue became softer (on the order of a 10% decrease in shear wave speed) when young wildtype mice transitioned from wake to anesthetized, yet this effect was lost in aging and with mice overexpressing amyloid-β or lacking the water channel AQP4. (2) Cortical stiffness increased with age in all mice lines, but wildtype mice exhibited the most prominent changes as a function of aging. The study provides novel insight into the brain's biomechanics, the constraints of fluid flow, and how the state of brain activity affects basic properties of cortical tissues.

A Novel 3D High Resolution Imaging Method Using Correlative X-Ray and Electron Microscopy to Study Neodymium-Doped Yttrium Aluminum Garnet Laser-Induced Defects in Intraocular Lenses.

Cataract extraction is the most frequently performed ophthalmological procedure worldwide. Posterior capsule opacification remains the most common consequence after cataract surgery and can lead to deterioration of the visual performance with cloudy, blurred vision and halo, glare effects. Neodymium-doped yttrium aluminum garnet (Nd:YAG) laser capsulotomy is the gold standard treatment and a very effective, safe and fast procedure in removing the cloudy posterior capsule. Damaging the intraocular lens (IOL) during the treatment may occur due to wrong focus of the laser beam. These YAG-pits may lead to a permanent impairment of the visual quality. In an experimental study, we intentionally induced YAG pits in hydrophilic and hydrophobic acrylic IOLs using a photodisruption laser with 2.6 mJ. This experimental study established a novel 3D imaging method using correlative X-ray and scanning electron microscopy (SEM) to characterize these damages. By integrating the information obtained from both X-ray microscopy and SEM, a comprehensive picture of the materials structure and performance could be established. It could be revealed that although the exact same energies were used to all samples, the observed defects in the tested lenses showed severe differences in shape and depth. While YAG pits in hydrophilic samples range from 100 to 180 µm depth with a round shape tip, very sharp tipped defects up to 250 µm in depth were found in hydrophobic samples. In all samples, particles/fragments of the IOL material were found on the surface that were blasted out as a result of the laser shelling. Defects in hydrophilic and hydrophobic acrylic materials differ. Material particles can detach from the IOL and were found on the surface of the samples. The results of the laboratory study illustrate the importance of a precise and careful approach to Nd:YAG capsulotomy in order to avoid permanent damage to the IOL. The use of an appropriate contact glass and posterior offset setting to increase safety should be carried out routinely.

Observing perineuronal nets like structures via coaxial scattering quantitative interference imaging at multiple wavelengths

Perineuronal nets (PNNs) are important functional structures on the surface of nerve cells. Observation of PNNs usually requires dyeing or fluorescent labeling. As a network structure with a micron grid and sub-wavelength thickness but no special optical properties, quantitative phase imaging (QPI) is the only purely optical method for high-resolution imaging of PNNs. We proposed a Scattering Quantitative Interference Imaging (SQII) method which measures the geometric rather than transmission or reflection phase during the scattering process to visualize PNNs. Different from QIP methods, SQII method is sensitive to scattering and not affected by wavelength changes. Via geometric phase shifting method, we simplify the phase shift operation. The SQII method not only focuses on interference phase, but also on the interference contrast. The singularity points and phase lines of the scattering geometric phase depict the edges of the network structure and can be found at the valley area of the interference contrast parameter SIND R under different wavelengths. Our SQII method has its unique imaging properties, is very simple and easy to implement and has more worth for promotion.

High-resolution acoustic imaging method based on equivalent source method and reweighted l1 minimization

Sparse regularization has been successfully applied to equivalent source method (ESM) in order to improve the acoustic imaging resolution. However, the application is not always feasible, especially at low frequencies. To overcome the problem, this paper proposes a high-resolution acoustic imaging method. In this method, reweighted l1 minimization is introduced to ESM to deal with the ill-posed inverse problems. Then the obtained equivalent source strengths are used to locate the sound sources. Compared to the sparse regularization-based ESM, the proposed method can provide a low side lobe and higher spatial resolution of acoustic imaging. Meanwhile, by arranging equivalent sources in three-dimensional space, the proposed method can also realize the acoustic imaging in three-dimensional sound field with high resolution. The results of the simulation and experiment demonstrate the validations.

Evaluation of the Bond Quality of Metal-Clad Plates Using Laser Ultrasonic Local Resonance

The effective detection of delamination defects, especially sub-millimeter delamination defects, in metal-clad plates is of great significance in improving product quality. In this work, the laser ultrasonic (LU) local resonance method is used to locate and characterize the sub-millimeter defects in stainless/carbon steel-clad plates. The influence of the delamination radius on the amplitude and resonant frequency of the laser ultrasound was investigated using 2D axisymmetric finite element (FE) simulations. The simulation results show that both the amplitude and the first resonance frequency (FRF) are effective features for detecting large-scale delamination defects, but the FRF is a better feature for detecting tiny delamination defects. A 304/Q235/304-clad plate specimen was made through a hot rolling bonding process, which contained a large number of self-forming delamination defects. The laser ultrasonic signals of different composite states collected in the experiment have good consistency with the simulated waveforms. The experimental results show that the laser ultrasonic local resonance method is a high-resolution imaging method, which can locate and characterize the sub-millimeter delamination defects in stainless/carbon steel-clad plates.

A multi-modal microscope for integrated mapping of cellular forces and Brillouin scattering with high resolution

Mechanical forces and stiffness play key roles in the health and development of cells and tissue, but despite the physical connection between these quantities, they cannot be monitored in parallel in most cases. Here, we introduce a fully integrated microscope that combines a method for high-resolution cell force imaging (elastic resonator interference stress microscopy, ERISM) with non-contact mapping of the elastic properties of cells (via Brillouin microscopy). In order to integrate both techniques, we had to account for the strong back reflection on the surface of the microcavity used for ERISM measurements as well as the local destruction of the cavity under illumination for Brillouin microscopy measurements. Therefore, we developed an elastic optical microcavity with minimal absorption that can perform ERISM measurements without sustaining laser damage during Brillouin microscopy. Furthermore, an unequal-arm Michelson interferometer was designed to suppress the back reflection of the laser on the ERISM microcavity surface using division by amplitude interference to reduce the reflected light and enhance the Brillouin signal. We show the utility of our integrated microscope by simultaneously mapping cellular forces and Brillouin shifts in cultures of fibroblast cells.

Deep learning-enhanced snapshot hyperspectral confocal microscopy imaging system

Laser-scanning confocal hyperspectral microscopy is a powerful technique to identify the different sample constituents and their spatial distribution in three-dimensional (3D). However, it suffers from low imaging speed because of the mechanical scanning methods. To overcome this challenge, we propose a snapshot hyperspectral confocal microscopy imaging system (SHCMS). It combined coded illumination microscopy based on a digital micromirror device (DMD) with a snapshot hyperspectral confocal neural network (SHCNet) to realize single-shot confocal hyperspectral imaging. With SHCMS, high-contrast 160-bands confocal hyperspectral images of potato tuber autofluorescence can be collected by only single-shot, which is almost 5 times improvement in the number of spectral channels than previously reported methods. Moreover, our approach can efficiently record hyperspectral volumetric imaging due to the optical sectioning capability. This fast high-resolution hyperspectral imaging method may pave the way for real-time highly multiplexed biological imaging.

Enhancing angular resolution in single-photon LIDAR for daytime long-range 3D imaging

Single photon LIDAR exhibits exceptional detection sensitivity and finds extensive applications in long-distance measurement and three-dimensional imaging domains. However, it is susceptible to background noise interference, leading to a trade-off between scanning imaging efficiency and angular resolution, thereby limiting its overall performance. In this paper, we propose a novel ‘compression-resampling’ high-resolution imaging method for single-photon scanning LIDAR based on the spatial correlation of the target scene. This approach significantly enhances the system's angular resolution without compromising imaging efficiency. By employing this method, we successfully conducted 3D imaging over distances exceeding 26 km even under challenging conditions with high levels of background noise during daylight hours. Within a target field of view measuring 1° × 1°, we reconstructed a high-resolution 3D point cloud image comprising 1000 × 1410 pixels. Notably, this achievement yielded an impressive angular resolution of 17.4 urad while maintaining an acceptable imaging time of approximately 20 minutes. The results unequivocally demonstrate the effectiveness of our proposed method in improving the quality of three-dimensional imaging and enhancing angular resolution while simultaneously reducing imaging time requirements, thus expanding potential application scenarios for single-photon LIDAR technology.

Lanthanide luminescence nanothermometer with working wavelength beyond 1500 nm for cerebrovascular temperature imaging in vivo

Nanothermometers enable the detection of temperature changes at the microscopic scale, which is crucial for elucidating biological mechanisms and guiding treatment strategies. However, temperature monitoring of micron-scale structures in vivo using luminescent nanothermometers remains challenging, primarily due to the severe scattering effect of biological tissue that compromises the imaging resolution. Herein, a lanthanide luminescence nanothermometer with a working wavelength beyond 1500 nm is developed to achieve high-resolution temperature imaging in vivo. The energy transfer between lanthanide ions (Er3+ and Yb3+) and H2O molecules, called the environment quenching assisted downshifting process, is utilized to establish temperature-sensitive emissions at 1550 and 980 nm. Using an optimized thin active shell doped with Yb3+ ions, the nanothermometer’s thermal sensitivity and the 1550 nm emission intensity are enhanced by modulating the environment quenching assisted downshifting process. Consequently, minimally invasive temperature imaging of the cerebrovascular system in mice with an imaging resolution of nearly 200 μm is achieved using the nanothermometer. This work points to a method for high-resolution temperature imaging of micron-level structures in vivo, potentially giving insights into research in temperature sensing, disease diagnosis, and treatment development.

Multi-Aperture Joint-Encoding Fourier Ptychography for a Distributed System

High-resolution infrared remote sensing imaging is critical in planetary exploration, especially under demanding engineering conditions. However, due to diffraction, the spatial resolution of conventional methods is relatively low, and the spatial bandwidth product limits imaging systems’ design. Extensive research has been conducted with the aim of enhancing spatial resolution in remote sensing using a multi-aperture structure, but obtaining high-precision co-phase results using a sub-aperture remains challenging. A new high-resolution imaging method utilizing multi-aperture joint-encoding Fourier ptychography (JEFP) is proposed as a practical means to achieve super-resolution infrared imaging using distributed platforms. We demonstrated that the JEFP approach achieves pixel super-resolution with high efficiency, without requiring subsystems to perform mechanical scanning in space or to have high position accuracy. Our JEFP approach extends the application scope of Fourier ptychographic imaging, especially in distributed platforms for planetary exploration applications.

A novel high-resolution imaging method based on sparsity in the time domain and spectral fitting in the frequency domain

During the propagation of seismic waves underground, the high-frequency seismic response of thin reservoir is absorbed and attenuated, which poses a challenge in seismic thin reservoir prediction. The high-resolution processing techniques have the capability to significantly expand the frequency range of the seismic data, so it becomes a key technique for thin reservoir prediction. Most of these techniques necessitate the extraction of seismic wavelets. However, the spatial and temporal variations of seismic data result in multiple solutions for wavelet extraction. Simultaneously, the majority of techniques fail to consider the influence of spatial tectonic features on the high-resolution processing. In this paper, we propose a novel solution to address these two fundamental challenges by utilizing seismic spectral expansion, sparse reflection coefficients, and spatial continuity constraints. First, we propose an innovative spectral fitting method that aims to expand the frequency bandwidth while adhering to the desired wavelet constraints. This method allows us to fully utilize the effective frequency information. It not only obtains broadband seismic data but also captures precise wavelets. Then, sparse deconvolution is employed to further extend the frequency range by utilizing the accurately expected wavelet and obtaining a high-resolution reflection coefficient. Finally, the Hessian matrix regularization is employed to constrain the spatial continuity of the reflection coefficient. This method is validated in both the model and real seismic data. Compared to traditional sparse deconvolution and spectral modeling deconvolution with spatial constraints, this method not only expands the frequency bandwidth and enhances seismic resolution but also preserves operational frequency information and improves the spatial continuity of seismic data. It has been verified that this approach can be used to forecast thin reservoir and reconstruct spatial tectonic characteristics.