Method For Determination Of Glycerol Research Articles

Chromatographic and Spectroscopic Method for Determination of Glycerol in Sanitizer Used During COVID-19 Pandemic

The use of sanitizers in the COVID19 pandemic is very common. While using sanitizers in Nepal, we have found people having problems with skin irritation and rashes; hence it was felt to know the amount of glycerol used as an emollient. A validated UV visible spectrophotometric and Reverse-phase High-performance liquid chromatography (RP-HPLC) method was designed to determine the amount of glycerol in the locally available sanitizers. The glycerol in sanitizers showed variation in amount, ranging from 0.78 to 1.66 g and 0.75 to 1.62 g/100 mL by HPLC and UV, respectively. The sanitizer samples with less purity or failed to meet the specification limit should be withdrawn, and their use must be limited. Hence this method seems to be easy, reliable, and cost-effective for determining glycerol based on the chemical derivatization technique.

Automated multiple development method for determination of glycerol produced by wine yeasts

A rapid and efficient analytical method for the determination of glycerol in wines is described. This method utilizes high-performance thin layer chromatography (HPTLC) plates coupled with an automated multiple development system with an elution gradient based on acetonitrile–acetone–hexane on silica gel layers. The absence of clean-up procedures, sometimes only centrifugation, makes this method suitable also for the large-scale control of alcoholic beverages. In particular the capacity of different wine yeast species (Saccharomyces cerevisiae, Zygosaccharomyces bailii, Kloeckera apiculata and Saccharomycodes ludwigii) to produce glycerol was determined. Generally, the strains of S. cerevisiae produced elevated amounts of glycerol together with Z. bailii, whereas K. apiculata strains formed the lowest amounts of glycerol, exhibiting also a great strain variability.

Liquid Chromatographic Method for Determination of Glycerol in Wine and Grape Juice: Collaborative Study

Abstract An Ion-exchange liquid chromatographic method for the determination of glycerol in wine, white grape juice, and pink grape juice was collaboratively studied by 8 laboratories. Eight wine types and 12 juice samples were provided to each collaborator. Using a strong cation column, blind duplicates and standards were analyzed by an external standard method. Separate statistical evaluations were run on wine, white grape juice, and pink grape juice data. The averages of the relative standard deviations for repeatability, excluding outlying results, were 1.25% for the wine samples, 7.32% for the white grape juice samples, and 8.63% for the pink grape juice samples. The averages of the relative standard deviations for reproducibility, excluding outlying results, were 2.79% for the wine samples, 16.97% for the white grape juice samples, and 19.10% for the pink grape juice samples. The method has been adopted first action by AOAC International.

Quantitative determination of glycerol in phospholipids by gas-liquid chromatography

1. A new method for the quantitative determination of glycerol entering into the composition of natural glycerophosphatides has been developed.