Method For Determination Of Glucosamine Research Articles

Development of a Simple and Sensitive High-Performance Liquid Chromatography Method for Determination of Glucosamine in Pharmaceutical Formulations

A column high-performance liquid chromatography (HPLC) method was developed for the determination of glucosamine in dosage forms. Glucosamine was derivatized by addition of a solution containing orthophthaldialdehyde. The HPLC separation was achieved on a Spherimage 80 ODS2 column (250 x 4 mm id, 5 microm particle size) using an isocratic mobile phase containing phosphate buffer-methanol (90 + 10, v/v, pH 6.50) and methanol-tetrahydrofuran (97 + 3, v/v) in proportions of 85 + 15 at a flow rate of 1 mL/min, followed by fluorescence detection. The method was validated for specificity, linearity, accuracy, precision, limit of detection (LOD), and limit of quantitation (LOQ). The detector response for glucosamine HCI was linear over the concentration range of 0.1-20 microg/mL with a correlation coefficient of 0.9980. The accuracy was between 99.4 and 100.8%. The LOD and the LOQ were 0.009 and 0.027 microg/mL, respectively. The method was applied to determination of glucosamine in solid dosage forms.

Spectrophotometric method for determination of glucosamine in tablets

A rapid and sensitive method has been developed for the determination of glucosamine sulphate from tablets by UV-spectrophotometery. In this method, glucosamine sulphate was reacted with phenylisothiocyanate in presence of a base to yield phenylthiourea derivative. This derivative showed maximum absorbance at 240 nm. Beer's law is obeyed in the concentration range of 5-25 mg/ml. The method was validated in terms of linearity, precision (relative standard deviation 1.1%), accuracy and specificity. The proposed method is the only method available for spectrophotometric determination of the drug. It is simple, precise, accurate, sensitive and reproducible and can be used for the routine quality control testing of the marketed formulations.

Single Laboratory Validation of a Method for Determination of Glucosamine in RawMaterials and Dietary Supplements Containing Glucosamine Sulfate and/or Glucosamine Hydrochloride by High-Performance Liquid Chromatography with FMOC-Su Derivatization

Single laboratory validation of a method for determination of glucosamine in raw materials and dietary supplements containing glucosamine sulfate and/or glucosamine hydrochloride by with high-performance liquid chromatography FMOC-Su derivatization. Tests with 2 blank matrixes containing SAMe, vitamin C, citric acid, chondroitin sulfates, methylsulfonylmethane, lemon juice concentrate, and other potential interferents showed the method to be selective and specific. Eight calibration curves prepared over 7 working days indicated excellent reproducibility with the linear range at least over 2.0-150 microg/mL, and determination coefficients >0.9999. Average spike recovery from the blank matrix (n = 8 over 2 days) was 93.5, 99.4, and 100.4% at respective spike levels of 15, 100, and 150%, and from the sample matrix containing glucosamine (n = 3) was 99.9 and 102.8% at respective levels of 10 and 40%, with relative standard deviations <0.9%. The method was also applied to 12 various glucosamine finished products and raw materials. The stability tests confirmed that glucosamine-FMOC-Su derivative once formed is stable at room temperature for at least 5 days. Limit of quantitation was 1 microg/mL and limit of detection was 0.3 microg/mL. The method is ready to proceed for the collaborative study.

Colorimetric Method for Determination of Glucosamine and Galactosamine

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTColorimetric Method for Determination of Glucosamine and GalactosamineSaul. Roseman and Irmgard. DaffnerCite this: Anal. Chem. 1956, 28, 11, 1743–1746Publication Date (Print):November 1, 1956Publication History Published online1 May 2002Published inissue 1 November 1956https://doi.org/10.1021/ac60119a028RIGHTS & PERMISSIONSArticle Views431Altmetric-Citations154LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (528 KB) Get e-Alerts Get e-Alerts