Method For Determination Of Curcumin Research Articles

Development of UV spectrophotometric method for estimation of curcumin in bulk drug and nanogel formulation: A hydrolytic degradation studies

The main purpose of this research was to develop and validate a cost effective UV spectrophotometric method for determination of curcumin in bulk drug and nanogel formulation. The method was validated according to the guideline of International Conference on Harmonisation (ICH). The curcumin was found to be soluble in methanol with λmax of 425 nm. Curcumin over the concentration level of (1-8 µg mL) depicted linear relation in the plot of concentration vs. absorbance. The regression equation of graph was y = 0.1137x - 0.0138 with R value of 0.999. The limits of detection and quantification were 0.23 μg mLand 0.75 μg mL, respectively. The method was accurate, precise, reproducible and robust since all the samples analyzed had relative standard deviation less than 2%. No statistically significant difference between theoretical and measured concentrations was detected. The degradation kinetics of free curcumin (k = 0.042 µg mL.h, 0.53 µg mL.h and 0.068 µg mL.h) and nanogel formulation (k = 0.178 h, 0.003 h and 0.005 h) were zero and first order, respectively. The proposed method was found to be specific while estimating curcumin loaded nanogel formulation without interference of excipients. The developed method was cost effective and can be used for routine quality control analysis of curcumin.

Reduced graphene oxide and carbon nanotubes composite functionalized by azobenzene, characterization and its potential as a curcumin electrochemical sensor

In this study, azobenzene-modified reduced graphene [email protected] carbon nanotubes ([email protected]) was synthesized and characterized by UV–Vis, FT-IR, XRD, FE-SEM, AFM, TEM, EDS, element mapping, BET, and cyclic voltammetry (CV). A glassy carbon electrode (GCE) was modified with [email protected] as an electrochemical sensor for determination of curcumin. Electrochemical impedance spectroscopy (EIS) and CV were used to study the efficiency of the catalyst. Subsequently, various effective parameters on the sensitivity and selectivity of the method, including pH, accumulation potential, and preconcentration time, were optimized. The transfer coefficient, as well as the electron transfer rate constant, were respectively calculated using the Laviron equation as 0.55 and 0.32 s−1. The linear ranges of the sensor were 0.008–2.0 and 2.0–10.0 μmol L−1 curcumin with a limit of detection of 0.003 μmol L−1 using square wave voltammetry (SWV). The repeatability and reproducibility were 2.0% (RSD) and 3.6% (RSD), respectively. The selectivity of the method for determination of curcumin in the presence of different interference species was studied too. Finally, the proposed sensor for determining curcumin was tested with real samples, including plasma, urine, and pharmaceutical compounds.

A NEW STABILITY-INDICATING RP-HPLC METHOD FOR DETERMINATION OF CURCUMIN: AN APPLICATION TO NANOPARTICULATE FORMULATION

<p class="Default"><strong>Objective: </strong>The present study was aimed to develop a rapid, accurate, linear, sensitive and stability-indicating high performance liquid chromatographic method for determination of curcumin and to implement the developed method for the estimation of curcumin in the nanoparticulate formulation.</p><p class="Default"><strong>Methods: </strong>Method development was performed using various solvent, buffer-solvent ratios, at different flow rates for better resolution and to decrease the run time. The developed method was validated in accordance with the international conference on harmonization (ICH) guidelines. The developed method was implemented to estimate the amount of curcumin in the curcumin-nanoparticulate formulation.</p><p class="Default"><strong>Results: </strong>The optimum chromatographic conditions with adequate resolution for curcumin (16.10 min) was achieved when the separation was carried using C<sub>18 </sub>column at ambient temperature with an isocratic elution mode of mobile phase composed of a degassed mixture of phosphate buffer pH 3 and acetonitrile (50:50 v/v) at 1.0 ml/min flow rate with a total run time of 20 min. The developed method was validated for system suitability, accuracy, precision, limit of detection (LOD), limit of quantitation (LOQ), linearity and range. The LOD and LOQ were found to be 0.018 and 0.056 μg/ml respectively, which indicates that the method was sensitive, and can detect and quantify at lower levels of curcumin. Linearity range was from 5-15 μg/ml for curcumin with regression coefficient 0.997 indicates that at this concentration range curcumin was highly linear. Percent assay of curcumin was found to be 98.7% and curcumin recovered was found to be 0.78 mg which are estimated by using the developed method.</p><p class="Default"><strong>Conclusion: </strong>The developed analytical method is simple, precise, and reproducible and thus can be used for stability-indicating analysis of curcumin in pharmaceutical formulations.</p>

Electrochemical Behavior and Voltammetric Determination of Curcumin at Electrochemically Reduced Graphene Oxide Modified Glassy Carbon Electrode

AbstractThis work presents a sensitive voltammetric method for determination of curcumin by using a electrochemically reduced graphene oxide (ERGO) modified glass carbon electrode (GCE) in 100 mM KCl‐10 mM sodium phosphate buffer solution (pH 7.40). The electrochemical behaviors of curcumin at ERGO/GCE were investigated by cyclic voltammetry, suggesting that the ERGO/GCE exhibits excellent electrocatalytic activity towards curcumin, compared with bare GCE and GO/GCE electrodes. The electrochemical reaction mechanisms of curcumin, demethoxycurcumin and bisdemethoxycurcumin at the ERGO/GCE were also investigated and discussed systematically. Under physiological condition, the modified electrode showed linear voltammetric response from 0.2 μM to 60.0 μM for curcumin, with the detection limit of 0.1 μm. This work demonstrates that the graphene‐modified electrode is a promising strategy for electrochemical determination of biological important phenolic compounds.

A simple reversed phase high-performance liquid chromatography (RP-HPLC) method for determination of curcumin in aqueous humor of rabbit.

This article describes a simple and rapid method for determination of curcumin (diferuloylmethane) in aqueous humor of rabbit using high-performance liquid chromatography (HPLC). Analysis was performed using a C-18 column (250 × 4.6 mm, 5 μ luna) by isocratic elution with a mobile phase containing 25 mM potassium dihydrogen orthophosphate (pH 3.5): Acetonitrile (40:60) and detection at 424 nm using a photodiode array (PDA) detector for curcumin. The regression data for curcumin showed a good linear relationship with r2> 0.998 over the concentration range of 0.1-10 μg ml−1. Relative standard deviations (RSD) for the intraday and interday coefficient of variations for the assay were less than 5.0 and 8.5, respectively. The recovery of the method was between 79.8-83.6%. The quantification limit of the method for curcumin was 0.01 μg ml−1. This method has good accuracy, precision, and quantitation limit. It is also concluded that the method is useful for measuring very low curcumin concentrations in aqueous humor.

Abstract 5494: A liquid chromatography-tandem mass spectrometric method for quantification of curcuminoids in cell culture medium and mouse plasma and their pharmacokinetics in mice

Abstract Curcumin and tetrahydrocurcumin (THC) have been found as potent DNMT1 inhibitors, but they suffer from low oral bioavailability and rapid metabolism in vivo. To circumvent these problems, two curcumin analogs: 1,7-bis (3,4-dimethoxyphenyl)-4,4-dimethyl-1,6-heptadiene-3,5-dione (TMC) and 1,7-bis(3,4-dimethoxyphenyl)-4-cyclohexyl-1,6-heptadiene-3,5-dione (DMCHC) have been synthesized to enhance their stability by blocking the two metabolic sites, the phenolic and C4 methylene moieties. Both compounds have shown inhibitory activity on M. SssI similar to that of curcumin and THC (Poster, M1114, AAPS, 2009). Preclinical pharmacokinetics has yet to be performed. Purpose: A sensitive and rapid LC-MS/MS method for simultaneous determination of curcumin and its metabolite THC and analogs TMC and DMCHC in RPMI cell culture medium and mouse plasma has been developed and validated and their stability in these matrices and their pharmacokinetics in mice were evaluated. Method: A triple quadrupole mass spectrometer with electro-spray (ESI) ionization was used for quantification. Spiked curcuminoids and the internal standard, hesperetin were extracted from mouse plasma with ethyl acetate, and the components were separated on a BetaBasic C8 column. The ion transitions at m/z 369→177, m/z 373→137, m/z 425→191, m/z 465→191 and m/z 303→153 for curcumin. THC, TMC, DMHC, and hesperetin, respectively, were monitored for their quantification. These curcuminoids were incubated in mouse plasma or cell culture at different temperatures and aliquots were removed at different times up to 24 hrs. Cassette dose of curcumin and TMC (10 mg/kg each) was intraperitoneally administered to CD1F2 mice and timed plasma samples were collected up to 8 hr. Curcuminoids in these samples were quantified using this method. Result: The lower limit of quantifications for all four curcuminoids was found to be 1 ng/mL in both mouse plasma and cell culture medium. The within-day coefficients of variation were found to be below 15% and the accuracy was in the range of 85% to 115%. Curcuminoids degraded in two phases with terminal half lives of 186, 813, 724, and 2000 min for curcumin, IHC, TMC, and DMCHC, respectively, in cell culture medium. In plasma, their respective half lives were 111, 232, 1202 and 3000 min. These data demonstrated that their stability are in the order of curcumin<THC<TMC< DMCHC in both matrices. Following an i.p. cassette dose, TMC showed more rapid absorption than curcumin (15 vs 60 min) and yielded a higher Cmax (0.30 vs 0.21 μM). Conclusion: A sensitive LC-MS/MS method for simultaneous quantification of these curcumionids has been established. TMC and DMCHC were more stable than curcumin in cell culture medium and mouse plasma. Further pharmacokinetic study is ongoing. Supported by NIH/NCI R21 CA135478, and BioMedical Mass Spectrometric Laboratory. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 5494.

Development and Validation of a Reversed Phase HPLC Method for Simultaneous Determination of Curcumin and Piperine in Human Plasma for Application in Clinical Pharmacological Studies

A sensitive and specific HPLC method was developed for the simultaneous determination of curcumin and piperine in human plasma in view of the potential therapeutic application of curcumin and piperine in various diseases. The HPLC method consisted of isocratic elution with acetonitrile-methanol-trifluoroacetic acid-water (17.6:35.3:0.1:47.0, v/v/v/v) with a flow rate of 2.5 mL min−1 on a Chromolith® SpeedROD RP-18 (50 × 4.6 mm) column at an ambient temperature. Ultraviolet detection was performed in programe mode at 415 nm for curcumin, 335 nm for piperine, and 280 nm for β-17-estradiol acetate (internal standard). Curcumin, piperine, and internal standard were extracted from plasma using ethyl acetate-propanol (9:1 v/v). Mean extraction recoveries for curcumin, piperine, and internal standard were 91.3, 91.4, and 92.9%, respectively. The assay was linear over the therapeutic concentration range (10–500 ng mL−1) for both drugs with correlation coefficients of r2 > 0.99. Limit of detection and limit of quantification were 1 ng mL−1 and 10 ng mL−1 for both curcumin and piperine. The method was applied to the determination of the concentrations of curcumin and piperine in healthy volunteers after treatment with 1500 mg curcumin and 500 mg piperine, and should find an applications in pharmacokinetic studies of these compounds.