Influence of drying temperature on the metabolites profile and potential antioxidant pathways of Passiflora edulis peel: Integrating untargeted metabolomics with network pharmacology analyses, molecular docking, and molecular dynamics simulation.

Acute high-temperature stress induces oxidative stress, ferroptosis, and metabolic homeostasis changes in the gills of Trachinotus ovatus.

This study investigated the reparative effects of Brassica rapa L. ethanolic extract (BREE) on H2O2-induced oxidative injury in PC-12 cells. Brassica rapa L., a cruciferous plant rich in bioactive flavonoids, exhibits potent antioxidant and neuroprotective properties against oxidative stress, a key pathological driver in neurodegenerative diseases. This study investigated the reparative effects of Brassica rapa L. ethanolic extract (BREE) on H2O2-induced oxidative injury in PC-12 cells, employing a multi-omics approach integrating LC-MS/MS metabolite profiling, molecular docking, and transcriptomic validation. BREE demonstrated robust radical-scavenging activity. Molecular docking identified direct interactions among flavonoids within BREE, suggesting a role in redox modulation. Subsequent experiments confirmed the activation of the PI3K/Akt, Keap1-Nrf2, and cell cycle pathways. Functionally, BREE significantly restored cell viability to 162.3% ± 6.9% of that in the control group. These findings validate BREE's dual action in scavenging ROS and reprogramming redox signaling networks, positioning it as a candidate for combating oxidative stress-associated neurodegeneration and as a potential functional food for age-related cognitive decline.

The impact of 1-MCP treatment combined with polyethylene film bag packaging on the quality of 'Shannong Su' pears during storage.

Metabolite profiling of organophosphate ester exposure and type 2 diabetes mellitus risk: A metabolomics study in an epidemiological setting.

Multi-omics dissection of large-size formation in Eriocheir sinensis: Insights from RNA, metabolite profiling, and ceRNA regulatory networks.

Untargeted metabolomic data of some Indonesian seaweed species (Halymenia durvillei, Gracilaria gigas, Caulerpa racemosa, and Palmaria palmata).

Kinetic modeling and LC-MS/MS-based evaluation of naringenin in elicited Commiphora wightii (Arnott.) Bhandari callus with computational targeting against Zika virus

Prebiotic potential of turmeric and fingerroot polysaccharides: modulation of human gut microbiota and metabolite profiles in in vitro colonic fermentation

Early-life exposure to endocrine-disrupting chemicals (EDCs) can interfere with brain development and contribute to long-term cognitive impairments. This study investigated whether hippocampal metabolite alterations at postnatal day 6 (PND6) are associated with behavioral outcomes in early adulthood following perinatal exposure to four EDCs: bisphenol F (BPF), butyl benzyl phthalate (BBzP), triphenyl phosphate (TPHP), and diisononyl cyclohexane-1,2-dicarboxylate (DINCH). Male and female rats were exposed in utero and during lactation, and hippocampal tissue was collected at PND6 for targeted metabolomics and untargeted lipidomics. Behavioral testing in adulthood using the Morris water maze assessed spatial learning (acquisition) and cognitive flexibility (reversal). BBzP exposure impaired acquisition learning, and BPF disrupted reversal performance in males. In females, both DINCH and TPHP led to increased latency during reversal. A multi-layered analytical framework was applied to explore associations between early metabolite and lipid profiles and later behavioral performance, including group-based comparisons, correlation analyses, and evaluation of biologically informed ratios. Results revealed sex- and domain-specific alterations in steroid and thyroid hormones, neurotransmitters, and PUFA-containing lipid classes, as well as changes in functional ratios and metabolite-metabolite coordination. These early metabolic disruptions were associated with increased escape latency in adulthood, suggesting long-term impacts on hippocampal function.

• Desmodesmus subspicatus was bioprospected for photosynthetic biogas upgrading. • Maximum biomass productivity of 83.4 mg/L/d was achieved at pH 9 and 1.5 gIC/L. • A self-flocculation of 85% was observed at high pH and alkalinity. • Self-flocculation was driven by pH, EPS biosynthesis and precipitating divalent ions. • A carbon balance resulted in the 86-89% carbon recovery at the end of the process. Photosynthetic biogas upgrading using algae presents a sustainable approach by co-generating biomethane via anaerobic digestion and valuable algal biomass. To enable application of this technology in temperate climatic conditions (with typical temperature of 5-20°C), suitable algal species should additionally grow optimally at alkaline pH (9-10) and sustain growth at high alkalinity of 1.5-2.5 grams inorganic carbon per litre (gIC/L). It should also facilitate low-cost harvesting. This study addressed these constraints by bioprospecting indigenous temperate algae and identified Desmodesmus subspicatus as a robust candidate for photosynthetic biogas upgrading. D. subspicatus tolerated pH 9-10 and alkalinity 1.5-2.5 gIC/L at 20°C in Bold's Basal Medium (BBM), thereby meeting key physiochemical requirements for this technology. The maximum biomass productivity achieved was 83.4 mg/L/d at an initial pH 9.0 and alkalinity 1.5 gIC/L, as compared to 68.4 mg/L/d at an initial pH 9.5 and alkalinity 2.5 gIC/L. However, precipitation of calcium (Ca 2+ ) and magnesium (Mg 2+ ) ions was observed at high pH and high alkalinity, limiting nutrient availability to the algae. The strain exhibited self-flocculation of 85% of cells within 3 hours due to pronounced secretion of extracellular polymeric substances (EPS), peaking at 182 mg EPS/gram biomass. Metabolite profiling showed a shift in carbon flux towards biosynthesis of carbohydrates (29-35%) at the expense of proteins under high alkalinity. Future strategies to improve the strain’s performance and process economics should address precipitation of divalent ions, employ mixotrophic cultivation, and use adaptive laboratory evolution techniques.

An integrated derivatization-LC-MS strategy reveals cell-type-specific chiral amino acid profiles.

Study on chronic toxicity, metabolic profiles and potential mechanisms of Aristolochiae Fructus in rats.

Comprehensive metabolic profiling of 82 unifloral Jamun honey samples from the Mahabaleshwar region (Apis cerana) was achieved using NMR spectroscopy, HPLC, solvent extraction, and targeted biophysical assays. This is by far one of the largest datasets characterised for a single type of honey. • Exceptionally low glucose and low total glucose+fructose content observed for the Jamun honey are among the lowest reported for honeys in general. These features are consistently observed across all 82 samples, yielding a high fructose-to-glucose ratio , indicative of a potentially low glycemic index . • NMR signatures showed minimal fermentation markers across all samples, demonstrating the intrinsic stability of Jamun honey despite its tropical origin. • HPLC analysis identified six phenolic acids and six flavonoids , including high levels of myricetin, kaempferol, and 4-hydroxybenzoic acid , underpinning the honey’s strong antioxidant capacity, confirmed through TPC, TFC, and antioxidant assays. • Melissopalynology revealed sparse Jamun pollen , emphasizing its limited reliability for floral authentication and demonstrating the need for metabolomics-driven approaches. • The study delivers the first robust reference dataset for saccharides, metabolites, and antioxidant markers in authentic Jamun honey, establishing a scientific foundation for quality control, fraud detection, and honey authentication . • The metabolic profile positions Jamun honey as a high-value medicinal honey , comparable to Manuka, with elevated antioxidant metabolites and relatively lower sugar levels. Jamun honey, produced by Apis cerana bees foraging on Syzygium cumini (Malabar plum), is traditionally recommended for diabetics and valued for its antioxidant and medicinal properties. This study analyzes 82 Jamun honey samples from Mahabaleshwar, India for a detailed metabolite profiling using NMR spectroscopy and HPLC. NMR spectroscopy was employed to profile the sugar composition of honey, enabling quantitative analysis of glucose, fructose, maltose, sucrose, and turanose. Polyphenols and flavonoids were isolated by solid-phase extraction and subsequently identified and quantified using HPLC. The antioxidant properties of the Jamun honey were further characterized through biophysical assays. NMR revealed generally lower glucose (25.91±0.28 g/100 g of honey) and lower total glucose+fructose levels (61.22±0.64 g/100 g of honey)- compared to other honeys- resulting in a high fructose-to-glucose ratio (1.36) and suggesting a potentially low glycemic index. All samples showed minimal fermentation markers (hmf at 0.0003±0.0007 g/100 g of honey, ethanol at 0.018±0.0006 g/100 g of honey), indicating excellent stability. HPLC and antioxidant assays confirmed high antioxidant capacity (85.10±3.50 %), with elevated levels of myricetin, kaempferol, 4-hydroxybenzoic acid, and nine other polyphenols. Pollen analysis showed low Jamun pollen content, underscoring limitations of melissopalynology for floral authentication. In the context of rising honey adulteration and honey quality control, this study provides a compositional reference for authentic Jamun honey using advanced NMR and data-based profiling approaches on a large sample set.

Targeting astrocytic Nrf2 by Trilobatin alleviates lipopolysaccharide-induced depressive-like behaviors and cognitive impairment in mice: Mechanistic insights into gut microbiota and metabolites modulation.

Integrating metabolomics and molecular dynamics simulation to elucidate the anti-atherosclerotic mechanisms of Gynostemma pentaphyllum.

Metabolite profiling identifies upregulation of specific glycerophospholipids and sphingolipids in imatinib-resistant cell lines.

Metabolomic profiling of sweet potato snacks enriched with agave fructans: Effects of vacuum impregnation on in vitro gut-associated metabolites.

Differential metabolic responses to hydrogen peroxide-induced oxidative stress in parotid and submandibular gland acinar cell lines.

A novel PGPR Streptomyces sp. for efficient phthalate biodegradation: Mechanistic insights and application in contaminated soil.