Sustainable production of β-caryophyllene from fructose and carbon dioxide in Cupriavidus necator H16 through pathway enhancement and global regulatory optimization.

Methyltransferase CclA-dependent control of secondary metabolite gene clusters and stress response in aspergillus nidulans.

Cold exposure impairs the muscle growth-promoting effect of nighttime-restricted feeding by desynchronizing mitochondrial energy supply rhythm in rabbits.

Glutathione- responsive Phototheranostic platform for imaging-guided enhanced oxidation photoimmunotherapy-ferroptosis synergistic hepatocellular carcinoma therapy.

Morus alba Linn. leaf as a food and drug homologs: chemical composition and structure, biological activity, mechanism of action, research progress on its biosynthetic pathway, and application challenges in future food

Multi-omics dissection of lignan diversity and therapeutic potential in Ocimum: Identification of diphyllin as an anti-inflammatory agent targeting TNF-α signaling.

Substitution of fishmeal in Sparus aurata feed with Hermetia illucens meal elaborated by different successive post-harvest processing methods; nutrient utilization and growth indices, effects on intermediary metabolism and digestive chitinolytic activity

A novel flavin-containing monooxygenase from Pseudomonas guineae enables efficient biosynthesis of indigo from indole via both enzymatic and cell factory approaches.

Arginine pathway unveiled: The crucial role of argC in Salmonella Derby's survival under salt stress.

Tracer-assisted shotgun lipidomics (TASL): A quantitative workflow integrating stable-isotope tracing with global lipidome profiling.

Branched-chain aminotransferase1 (BCAT1) and BCAT2 initiate branched-chain amino acid catabolism, which provides precursors for multiple metabolic pathways. Yet, whether their degradation products converge on the same or diverge into distinct metabolic pathways remains an open question. In Arabidopsis thaliana, disruption of AtBCAT1 (but not AtBCAT2) leads to a deficiency in iso-branched waxes, demonstrating its specific role in their biosynthesis. Expressing AtBCAT2 from the AtBCAT1 promoter fails to rescue this defect, proving that AtBCAT1 uniquely channels Valine-derived carbon into wax synthesis. Phylogenetic analysis places BCAT1 and BCAT2 in separate clades that arose from an ancient tandem duplication. Rapeseed BnBCAT2, like Arabidopsis AtBCAT2, cannot complement bcat1, whereas BCAT2 orthologues from rice and tobacco partially rescue bcat1, underscoring Brassicaceae-specific sub-functionalization of BCAT1 that likely originated in a common ancestor. In addition, under darkness, AtBCAT1 delays dark-induced senescence, whereas AtBCAT2 accelerates it. This reveals that under energy-deficient conditions, AtBCAT2 supports energy production via the TCA cycle, while AtBCAT1 is preferentially diverted to alternative biosynthetic pathways. Collectively, AtBCAT1 and AtBCAT2 play distinct roles in branched wax synthesis and ATP production; that is, AtBCAT1 is dedicated to supplying iso-branched-chain wax precursors, whereas AtBCAT2 preferentially feeds the resulting carbon skeletons into the TCA cycle under energy-deficient conditions.

Natural cadinane-type sesquiterpenoids and their bioactivity: a review (2015-2025).

Enzymatic basis underlying the biosynthesis of Δ7-desaturated C22 polymethylene-interrupted fatty acids across marine invertebrates.

Transcriptomic analysis of the dynamic changes in polysaccharide biosynthesis metabolism in Lentinula edodes throughout liquid fermentation.

The nucleotide biosynthetic pathway is essential for tumor replication through DNA and RNA synthesis, making it a critical target for cancer therapies. Mebendazole (MBZ) has shown promise in inhibiting cell proliferation and glucose metabolism in gastric cancer (GC) cell lines. However, its impact on nucleotide synthesis remains unclear. This study investigates MBZ's role in nucleotide biosynthesis and its contribution to antiproliferative effects by modulating key DNA/RNA synthesis targets. MBZ demonstrates antitumor activity similar to 5-FU in GC cells, with lower toxicity to non-tumor cells. Gene expression analysis revealed overexpression of nucleotide metabolism genes (PRPS1, TYMS, MTHFD1, and HPRT1) in tumor samples, and higher levels of TYMS, MTHFD1, and HPRT1 were associated with poor survival in GC patients. MBZ treatment reduced the expression of these genes, induced G0/G1 phase cell cycle arrest, and inhibited metastatic cell proliferation after 48h. Molecular docking studies indicated that MBZ binds more strongly to PRPS1 and HPRT1 than their natural ligands or inhibitors. Our findings suggest that MBZ modulates nucleotide synthesis pathways, contributing to its selective antiproliferative effects in GC cells. This study highlights potential new pharmacological targets for drug repurposing and further investigation into the broader therapeutic applications of MBZ.

Metabolic reprogramming and intracellular ATP homeostasis in immunity.

Metagenomics-based insights into the microbial community composition and quality characteristics development potentiality in traditional dry-cured ham.

Chloro-containing polyketides with anti-neuroinflammatory and anti-angiogenic activities from a coralline algicolous fungal strain Diaporthe middletonii Km3279.

Understanding metabolic processes of soil fungi is essential for elucidating their ecological roles in biogeochemical cycles and responses to emergent environmental stressors. Here, we demonstrate the potential of using stable isotope probing Raman (SIP-Raman) microspectroscopy in microfluidics technology-based soil chips to trace glucose metabolism rates and stress responses in laboratory grown filamentous soil fungus Psilocybe cf. subviscida. The time evolution of Raman spectral band intensities resulting from deuterated glucose uptake in the fungal hyphae allowed us to assess glucose metabolism rates. Under excess copper (Cu) stress, we observed suppression of both glucose metabolic activity and growth. In addition, reduced spectral signatures of intracellular cytochrome c further implied impaired mitochondrial function and potential onset of cell death. However, laser-induced radiation damage hampered repeated Raman measurements, including multispectral mapping, on individual hyphae, especially when exposed to the Cu stress. To overcome this, we employed stimulated Raman scattering (SRS) microscopy, which offers much higher sensitivity and mapping speeds, and therefore much lower radiation doses. This enabled localization of the uptaken glucose at the inner edges of the P. cf. subviscida hyphae and Cu-induced formation of putative vacuolar structures. While integration of this approach with soil chips requires future modifications to the chip design for increased optical transparency and ensured sterility, overall, our results demonstrate the potential of Raman-based microspectroscopy for spatially resolved, in situ analysis of fungal primary metabolism and stress physiology.

Molecular mechanisms of tobacco in response to whitefly feeding at different developmental stages and evolutionary study of key AP2 transcription factors.