Soil organic matter affects a number of soil processes and properties. A better understanding of soil-profile distribution of organic matter components and related soil properties under long-term tillage systems is thus needed. The objective of this study was to evaluate the impacts of 33 years no-till (NT), double disk (DD), chisel (CH), and plow tillage (PT) under corn (Zea mays L.)–soybean (Glycine max L.) rotation on soil organic C (SOC), particulate organic matter (POM), pH, and wet aggregate stability to 100cm soil depth on a Sharpsburg silty clay loam (fine, montmorillonitic, mesic Typic Argiudolls) in eastern NE. After 33 years, NT and DD management increased SOC by 1.2 times and mean weight diameter (MWD) of aggregates by 2 times compared with CH and PT at the 0–10cm depth. At the 0–20cm, NT had 1.1 times higher SOC concentration than CH and PT. When compared with data collected 24 years prior to this study, SOC at the 0–20cm increased by 12.5% across NT, DD, and CH and by 2.7% for PT. No-till had 5 times higher total POM concentration than PT, 4.7 times higher than CH, and 2.4 times higher than DD at the 0–10cm depth. However, at the 10–20cm, PT had higher POM than other tillage systems, which is most probably due to mixing and burial of residues at the bottom of the plow layer. Soil pH did not differ among tillage treatments at the 0–10cm, but it differed in this order: PT>CH>DD>NT at the 10–20cm and PT=CH=DD>NT at the 20–40cm depth. The lower pH under NT, DD, and CH in deeper soil depths may be due to the limited or no lime mixing in these systems compared with PT. When compared with data (pH 5) collected 33 years prior to this study, soil pH increased by 0.9 in NT, 1.4 in DD, 1.5 in CH, and 1.9 units in PT at 0–20cm depth, probably due to surface application and incorporation of lime. Overall, 33 years of NT increased near-surface soil organic matter components and soil aggregation compared with the PT.