Mentik Wangi Research Articles

Volatile compounds characteristics of several Indonesian indigenous rice varieties

Abstract Information on the volatile compounds of scented/aromatic rice is crucial for plant breeders and food chemists in developing the rice varieties. One of the major hurdles in the improvement of aromatic rice through breeding in Indonesia is the lack of quantitative assay of volatile compounds. The objectives of the study were to characterize and identify volatile compounds that contribute to the flavor of aromatic rice. Twenty rice varieties were collected from four provinces in Indonesia, consisting of five varieties from North Sumatera, three varieties from South Sumatera, eight varieties from East Java, and four varieties from South Kalimantan. The rice volatile compounds were extracted by liquid-liquid extraction and then analyzed by Gas Chromatography-Mass Spectrometry. The GCMS analysis identified 94 volatile compounds from 8 compound classes, namely aldehydes (14 compounds), alcohols (20 compounds), ketones (15 compunds), benzenes (16 compounds), esters (15 compounds), acids (3 compounds), terpenes/mono+sesquiterpen (7 compounds), and aliphatic hydrocarbons (4 compounds). Mentik Wangi variety from East Java Province had the highest aroma impact compound of aromatic rice (2-acetyl-1-pyrroline), followed by Siudang and Pandanwangi varieties (East Java). Another rice flavor compound, hexanal, that contributed to the unpleasant odor of rice (green or grass-like odor), was also found in Kuku Balam variety (North Sumatera) with the highest concentration among others. It was followed by Seluang variety (South Sumatera) and Cantik Manis variety (North Sumatera). There were five varieties, namely Pandan Wangi, Cantik Manis, Siudang, Serendah, and Mentik Wangi, can be used as the parent for improving aromatic rice variety.

Relationship between chlorophyll and yield of two rice varieties in different farming systems

Abstract Rice is the staple food of Indonesian society which continues to increase in demand due to population growth. Currently, there are 3 agricultural systems in rice cultivation, namely organic, semi-organic, and conventional. The three systems provide changes in soil physical, chemical, and biological properties, but there is not much information related to chlorophyll levels in plants. This study aims to assess chlorophyll levels and their relationship with the yield of two rice varieties in various farming systems, especially the IR64 and Mentik Wangi varieties. This research is a field survey study followed by laboratory analysis. Determination of sampling locations by purposive sampling on organic, semi-organic, and conventional rice fields with two varieties of rice. Observation parameters include chlorophyll content, harvest dry grain weight, and milled dry grain weight. The results showed that the levels of chlorophyll A in IR64 and Chlorophyll B in IR 64 and Mentik Wangi varieties with organic farming systems were higher than in semi-organic and conventional farming systems. There was a positive correlation between chlorophyll levels and the yield of two rice varieties in different farming systems.

The Role of Net Assimilation Rate and Nitrogen Management in Optimizing Rice (Oryza sativa L.) Yield

Background: The net assimilation rate (NAR) is a physiological parameter related to leaf area and dry weight per unit of time. It is used to figure out how much grain a plant will produce. This study aims to explain the net assimilation rate of several types of rice at different doses and nitrogen application times. Methods: The research was conducted from December 2021 to April 2022. The experimental design was a split-plot design consisting of two treatment factors: the variety (main plot) and the time and dose of fertilization N (subplot). The variety factor consists of four varieties: IPB 3S (new type varieties), Inpari 33 (new high-yielding varieties), Hipa 21 (hybrids) and Mentik Wangi (local) at nitrogen 4 levels, namely 0 kg ha-1 (N0), 45 kg ha-1 applied when planting (N1), 90 kg ha-1 applied twice [45 kg at planting and 45 kg at panicle initiation (N2)] and 90 kg ha-1 applied three times, namely 45 kg at planting, 22.5 kg at the age of panicle initiation and 22.5 kg at heading (N3). Result: Results demonstrated that applying 90 kg ha-1 nitrogen fertilizer in three split applications (N3) improved agronomic performance by increasing tiller number, leaf area, dry matter accumulation, leaf nitrogen content and grain yield compared to other nitrogen regimes. Our findings newly highlight the vital influence of net assimilation rate (NAR) on panicle development, as NAR was positively correlated with panicle length. Uniquely, among all four varieties tested, the local cultivar Mentik Wangi recorded the highest NAR across N treatments.

Validation of molecular markers associated with amylose content of F8 and F9 lines resulting from a crossing of Black Rice and Mentik Wangi

The development of black rice with a fluffier texture at Universitas Jenderal Soedirman, which began in 2004, was carried out by crossing local black rice with the Mentik Wangi variety. At present, six black rice advanced breeding lines are ready for multi-location yield trials. Meanwhile, developing molecular marker-based plant breeding methods can facilitate a more efficient selection process. An important stage in developing molecular markers is marker validation, which aims to test the effectiveness in determining phenotypes in various genotypic and in new populations that differ from others based on the link between the marker and the expected character identified. This research aimed to determine molecular markers that can differentiate the level of amylose content in populations of F8 and F9 lines. The research was carried out on 10 (ten) genotypes consisting of the control variety Logawa, IRPM 112-19- 56, black rice, Mentik Wangi, and six lines. The amplification of the RM190, Wx and SSIIa markers showed polymorphic band. Based on the simple linear regression analysis, in F9, the SSIIa marker had the highest coefficient of determination compared to other markers. Hence, it had the greatest possibility of being a marker for selecting rice based on amylose content.

Differences in Brand Image Perceptions of Beras Kami and Mentik Wangi Rice among Bulog Kancab Semarang Customers

This research aims to determine consumer perceptions of the brand image of Beras Kita and Beras Mentik Wangi, especially customers of the Perum Bulog Semarang Branch Office. The research method used in this research is observation and questionnaires distributed to consumers. Observations were carried out during the internship activities, namely when participating in the bazaar and questionnaires were distributed to customers where the sample taken was 65 respondents. The six indicators used in this questionnaire are awareness, quality, CSR, social image, geographical origin, and uniqueness. The results of this research show that there is a significant difference in perception between Beras Kita and Beras Mentik Wangi where consumers have the perception that Beras Mentik Wangi has a better brand image than Beras Kita. Improvements are needed to increase consumer perceptions of Beras Kita so that in the future customers will judge that Beras Kita has a positive brand image. The results of this research show that there is a significant difference in perception between Beras Kita and Beras Mentik Wangi where consumers have the perception that Beras Mentik Wangi has a better brand image than Beras Kita. Improvements are needed to increase consumer perceptions of Beras Kita so that in the future customers will judge that Beras Kita has a positive brand image. The results of this research show that there is a significant difference in perception between Beras Kita and Beras Mentik Wangi where consumers have the perception that Beras Mentik Wangi has a better brand image than Beras Kita. Improvements are needed to increase consumer perceptions of Beras Kita so that in the future customers will judge that Beras Kita has a positive brand image.

Ecophysiology of Growth and Yield of Several Local Rice Varieties (Oryza Sativa L.) withVarious Kinds of Irrigation

A research aims to examine the physiology, growth and yield of rice in several varieties of rice plants with System of Rice Intensification and conventional irrigation methods. The study was conducted using 3x4 factorial strip plot design arranged in a Completely Randomized Block Design with 3 blocks as replications. The first factor was the type of irrigation that had 3 treatments, i.e., irrigation, intermittent irrigation: 10 days inundated, 5 days drained and intermittent irrigation: 7 days flooding, 3 days drying. The second factor was the rice varieties consisting of 4 varieties, i.e., Rojolele Genjah, Mentik Wangi, Mentik Susu and Cempo Hitam.The results showed that the plant height of the Rojolele Genjah variety was higher than other varieties. The grain yield per hectare of Mentik Wangi, Mentik Susu and Cempo Hitam varieties were higher than the Rojolele Genjah Variety. The treatment of irrigation for 7 days of flooding and 3 days of drying was greater than other irrigation for panicle length, and harvest index. CGR of conventional irrigation treatment was greater than intermittent irrigation. There was interaction between varieties and irrigation on the leaf area of rice plants, which in Cempon Hitam Variety with irrigation intermittently 10 days of flooding 5 days drying had the broadest leave area.

Construction of the CRISPR/Cas9 module and genetic transformation of aromatic rice cv. Mentik Wangi for developing bacterial leaf blight resistance

Abstract. Rifhani NF, Apriana A, Sisharmini A, Santoso TJ, Trijatmiko KR, Slamet-Loedin IH, Yunus A. 2023. Construction of the CRISPR/Cas9 module and genetic transformation of aromatic rice cv. Mentik Wangi for developing bacterial leaf blight resistance. Biodiversitas 24: 3258-3268.Rice cv. Mentik Wangi is a local aromatic rice that is susceptible to pests and diseases, such as bacterial leaf blight (BLB) caused by Xanthomonas oryzae(Xoo). This bacteria can cause damage to plants thereby reducing crop yields. This study aimed to obtain the CRISPR/Cas9 module construct as well as introducing the construct into rice cv. Mentik Wangi for developing BLB resistance. The manufacture of the CRISPR/Cas9 module was carried out using the Golden Gate method, and the introduction of that construct into rice cv. Mentik Wangi was carried out using Agrobacterium tumefaciens. Constructing a CRISPR/Cas9 module with multiple gRNA of OsSWEET11 and OsSWEET14 genes has succeeded, 129 putative transformant lines of T0 generation produced with the regeneration and transformation efficiency values ??are respectively 9.4% and 9.8%. The results showed that 36 lines were positive for the hpt gene, indicating that the CRISPR/Cas9-gRNAOsSWEET module construct was successfully entered into rice cv. Mentik Wangi. Further analysis is needed to identify mutagenesis in the target gene region of TI generation transgenic plants as well as phenotypic tests for BLB resistance.

KEMAMPUAN PENYERAPAN HARA PADA BERBAGAI VARIETAS PADI DAN SISTEM PENGAIRAN DI KABUPATEN DELI SERDANG

This journal contains research that aims to determine the ability of rice plant tissues in several varieties to absorb nutrients in the soil and in various irrigation systems in Deli Serdang Regency. This study used an Randomized Block Design (RAKL) with the first factor consisting of 2 levels, namely P1 (intermittent irrigation method) and P2 (continuous irrigation method). The second factor consisted of 4 varieties, namely Mentik Wangi, IR-64, Segara Anak, and Cianjur. The data from the analysis showed that the Cianjur variety Ca nutrient absorption and plant dry weight at the 13th week was influenced by various rice varieties and irrigation types, compared to other varieties. The conventional irrigation system showed Ca uptake and plant dry weight 13 weeks higher than SRI irrigation.

Identification Texture of Rice Varieties by Feature Extraction using GLCM

Rice is one of the components of staple ingredients included in the issue of world food security in the Sustainable Development Goals (SDGs) program. A large number of varieties of rice types allows deviations in the field by mixing good rice varieties with other varieties to increase profits. This problem causes consumers to experience economic losses; on the other hand, distinguishing rice varieties is difficult to do directly only through eyesight. This study tries to make an initial approach to get the similarity value of each rice-type texture. This study discusses the extraction of texture features in 3 rice varieties, including organic rice, Mentik Wangi, Rojo Lele, and Basmati India. The feature extraction method used is the Gray Level Co-occurrence Matrix (GLCM) for assessing the texture of an image variety of rice and evaluated with PSNR and MAE.

Profiling of Phytochemical Compounds of East Java Red Rice Bran Has the High-Value Biological Activities as Antioxidant and Antidiabetic

The phytochemicals contained in rice bran, mainly flavonoid compounds, are predicted to have biological activity. Flavonoids are able to counteract the free radicals and degrade insulin resistance. The East Java Red Rice Bran samples, e.g., Mentik Wangi, Aek Sibundong, and Blambangan, were used in the study. Their phytochemical profiles, functional groups, antioxidant, and antidiabetic activities were investigated. The phytochemical analysis showed that the bran of Mentik Wangi, Aek Sibundong, and Blambangan contained flavonoid, triterpenoid, phenolic, tannin, and glycoside. Based on the FTIR, some functional groups were identified in three rice bran varieties, namely, the O-H stretching, C-H aliphatic, C-H sp3 stretching, C=C stretching aromatics, C=C stretching alkenes, CH2 and CH3 bonds rocking, C-H aromatic, CH-OH stretching alcohols, and C-O stretching ether or ester suggesting that rice brans are rich in phytochemical compounds. Through LC-HRMS analysis in positive ion mode, several types of flavonoids were confirmed. Pinocembrin was found in the three brands. The highest antioxidant and antidiabetic activity were observed in Blambangan rice bran with an IC50 value of 1.09 and 75.76 µg/mL, respectively. To conclude, the red rice bran phytochemical compounds exhibit potential biological activities as antioxidant and antidiabetic agents.

Genetic Parameters, Inter-relationship Among Agronomic Traits and Dehulled Rice Morpho-Biochemical Profile of Promising Black Rice x Mentik Wangi Lines

A comprehensive understanding of the genetic parameters and the interrelationship among characters in the breeding population is crucial for selecting low amylose and high antioxidant black rice varieties. Meanwhile, dehulled rice morpho-biochemistry profile can be used to determine the grain quality of F6 and F7 lines of Black Rice x Mentik Wangi var. The objectives of this study were to determine the agronomic traits, figure up the genetic parameters, describe the relationship among agronomic characteristics of the F6 lines, and determine the morpho-biochemical profile of F7 dehulled rice. Agronomic traits showed a difference in each line. Genetic parameters in each trait showed various categories. Path analysis showed that the number of tillers affected the dry weight and grain weight per panicle, but the plant dry weight did not directly affect the weight of 1,000 grain. Directly, the weight of 1,000 grain was only significantly affected by the grain weight per panicle trait. The F7 lines had a difference in grain length and amylose content. The dehulled rice color of the two lines showed the combination of the two parents. Based on the student T-test conducted on F6 and F7 grain, there was no difference in antioxidant content between the two sample groups. The antioxidant activity of all lines was in the range between the antioxidant activities of the two checked varieties. Although further research is still needed, the lines have the potency to be developed as low amylose pigmented rice.

Expression of cytokinin responsive and ethylene biosynthesis genes in rice callus with different regeneration rates

This study aimed to investigate the correlation between callus regeneration rate and the expression of several genes responsible for cytokinin response and ethylene biosynthesis in the Ciherang, Mentik Wangi Susu, Hwayoung and Tarabas rice varieties. The callus regeneration rate of each rice variety was in vitro tested using N6 media, while the gene expression during the callus regeneration stages was examined using quantitative real‐time PCR (qRT‐PCR). Our results showed that the callus of Ciherang and Mentik Wangi Susu showed earlier green spot formation that then turned brown at a later stage, resulting in a low regeneration rate. While Hwayoung and Tarabas showed late green spot formation, high shoot regeneration was observed in both calluses. Gene expression analysis of regeneration media‐grown calluses showed that two cytokinin‐responsive genes, OsRR2 and OsRR6, were highly expressed in the Ciherang and Hwayoung callus, respectively. We also observed that ethylene biosynthesis genes such as OsACS1 and OsACO1 were highly expressed in the Mentik Wangi Susu and Hwayoung callus, respectively. Moreover, the expression of OsBBM1 was high in Hwayoung and Tarabas. Thus, the positive correlation between the expression of cytokinin‐responsive and ethylene biosynthesis genes with somatic embryogenesis activity likely depends on the induction level of OsBBM1.

Construction of CRISPR/Cas9_gRNA-OsCKX2 module cassette and its introduction into rice cv. Mentik Wangi mediated by Agrobacterium tumefaciens

Abstract. Ruzyati M, Sisharmini A, Apriana A, Santoso TJ, Purwanto E, Samanhudi, Yunus A. 2022. Construction of CRISPR/Cas9_gRNA-OsCKX2 module cassette and its introduction into rice cv. Mentik Wangi mediated by Agrobacterium tumefaciens. Biodiversitas 23: 2679-2689. Mentik Wangi is an aromatic superior rice variety from the tropical japonica group with high posture and low productivity. The tall plant posture causes Mentik Wangi rice to be prone to lodging, resulting in yield loss. Therefore, improvement of plant height and productivity of Mentik Wangi is still required. The sd-1 (OsGA20ox-2) and CKX2 genes are responsible for the semi-dwarf character and high productivity. The study aimed to construct a CRISPR/Cas9 cassette module carrying a gRNA of OsCKX2 gene and introduce this construct to Mentik Wangi rice mediated by an Agrobacterium tumefaciens vector strain LBA4404. The introduction of the CRISPR/Cas9_gRNA-GA20ox-2 cassette plasmid that was constructed in previous research into Mentik Wangi rice was also carried out. The results showed that the CRISPR/Cas9_gRNA-CKX2 cassette module had been successfully constructed using the Golden gate cloning method. The introduction of the CRISPR/Cas9_gRNA-CKX2 and CRISPR/Cas9_gRNA-GA20ox-2 cassette modules into Mentik Wangi rice resulted in 30 putative transformant lines that passed the hygromycin selection. PCR analysis showed that from the 30 transformant lines, 15 lines were positive for the hygromycin resistance gene. Further analysis is necessary to be conducted to identify the occurrence of mutagenesis in the OsCKX2 and GA20ox-2 target genes.

The Yield of M7 Mentik Wangi Rice Irradiated With Several Doses of Gamma Rays Under Drought Stress

The possible risk of yield losses due to drought stress could threaten rice production and national food security. One way to deal with drought limitations is by developing drought- tolerant varieties using gamma-ray irradiation. This research aimed to get the optimal dose of gamma-ray radiation to create an M7 generation of Mentik Wangi variety that is drought tolerant. Field experiments were conducted from December 2020 to March 2021 at the Screen House of the Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, the Laboratory of Soil Physics, and the Laboratory of Plant Production Management, Faculty of Agriculture, Universitas Gadjah Mada. This study has carried out using a Randomized Complete Block Design with two factors. The first factor is gamma-ray irradiation dose (0gy. 100gy, 200gy, 300gy), and the second factor is drought stress treatment (without drought stress and drought stress in the flowering stage) with three replications. The results showed no interaction between gamma-ray irradiation dose and drought stress on all observed variables except plant dry matter. Drought stress in the flowering stage increased the percentage of unfilled grain, reducing the harvest index and grain weight significantly by 40.9%. Gamma-ray irradiation in a range of 100 to 300 gy did not affect panicle numbers and length, also the percentage of unfilled grain, but reduced the number of grains per panicle, thereby reducing the harvest index and grain weight 45.0% compared to those without irradiation.

Growth performance of 'Mentik Wangi' rice (Oryza sativa L.) with Zn nano application on drought stress

Mentik wangi rice (Oryza sativa L.) is a variety originating from Magelang Regency, Central Java. Mentik wangi has the advantages of fragrant smell, fluffier texture, and high market demand. The weakness of mentik wangi rice is long life (4 months), long stems, easy collapse. The problem of long-lived rice if the planting period enters the dry season and the availability of water is limited, it will have an impact on productivity. This study aims to overcome problems by applying Zn nano to drought stress. Zn is one of plant microelements that functions as auxin biosynthesis, cell elongation, stem segments, and enzyme activator. Enzyme activators function in slowing the increase in reactive oxygen species (ROS) during drought stress, maintaining physiological resistance and rice growth. The study was conducted with a factorial completely randomized design in a screen house. The results showed Zn nano maintains yield and plant growth under stress. The most optimal dose. However, there is a tendency at the 3.75 ppm Zn nano dose to get low yields, it is suspected that if an excessive dose of Zn causes poisoning in plants. The application of Zn nano is optimal when applied under stressed conditions compared to normal conditions.

The growth and production of field rice (Oryza sativa l.) Inpari 9 and Mentik Wangi variety on peat land and peat buried

Rice plant is paddy producing plant needed by most of Indonesians as staple food. Sei Geringging Village in Kampar District, Kampar Kiri Sub-District had technically irrigated field on peat land and peat buried. Farmers on Sei Geringging Village farmed 2 kinds of rice which were prime variety and local variety. Most of prime variety farmed was Inpari 9 and the local variety was Mentik Wangi. Farmers on Sei Geringging Village had been farming the rice without knowing which variety had been best to farm on peat land and on peat buried that the yield had not been optimal. This research aimed to know the comparison of maximum growth and production rate of Inpari 9 and Mentik Wangi variety rice on peat land and peat buried. This research was done by a survey with descriptive method .Treatment combination was repeatedly done 4 times resulting 16 unit trial squares. Each square had 5 sample plants. Best growth was on peat land that planted with the Inpari 9 variety and on peat buried was planted with Mentik Wangi variety resulting yield of 4,20 ton.ha-1

Evaluation of the crossings between local and drought-tolerant rice varieties using simple sequence repeat (SSR) molecular marker

Two cultivars of local rice, namely Mentik Wangi and Mentik Susu, have been grown around west to south flank of Merapi volcano. They are highly valued for their good taste and cooking characteristics. An attempt to introduce drought-tolerant rice varieties has been conducted by crossing them with two sources, namely Kasalath and Bluebonnet. Molecular-assisted selection using a set of SSR markers was applied to check the F1 and F2 generation trueness and segregation. This research was conducted in the facilities of Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta. The research was a field experiment arranged in a completely randomized design, consisting of Mentik Wangi, Mentik Susu, Bluebonnet, and Kasalath, as well as their crossings’ F1 and F2 plants as treatments. The SSR markers used are RM72, RM228, RM518, and RM20(A). Polymorphism test of the parents showed that polymorphism exists between local and donor parents, thus these markers were considered eligible for the F1 and F2 tests. The heterozygous individuals of F1 were 75% for Bluebonnet × Mentik Wangi, 44.4% for Bluebonnet × Mentik Susu, and 46.7% for Mentik Wangi × Bluebonnet; all were consistent in every primer used. We could not confirm that the F2 populations showed segregation pattern that followed Mendelian segregation in some crosses due to too small size of the sample. Heterozygous individuals in F2 showed the differences pattern for each marker, indicating that the location of the SSR markers were far from each other in the genome.

Amylose profile and rice grain morphology of selected F6 lines derived from a crossing of Black Rice and Mentik Wangi for the development of waxy pigmented rice

Research on rice plant breeding to get superior black rice varieties with tender rice texture can be carried out by crossing Black Rice and Mentik Wangi variety. The rice lines derived from a crossing of these two varieties have recently reached the F6 line. The texture of rice is distinguished by the amylose content. The lower of the rice amylose, the more tender of the rice texture, and vice versa. This study aimed to find out the profile of amylose content and the grains morphology of the lines that will be developed as waxy pigmented rice. Analysis of amylose content was carried out using iodine-colorimetry methods. The quantification of amylose was measured based on the regression of the standard amylose curve. The results showed that all the F6 lines had the potential to be developed as waxy pigmented rice. Based on the Kruskall Wallis test, there was a variance in the amylose profile average of the eight genotypes. In terms of grains morphology, the line 482-17-7 and 482-17-18 had a combination colour between the two parents. In addition, there was a significant difference in grains size between the genotypes and the grain size of all lines were classified in the medium size. F6 lines resulted from the crossing between Black Rice and Mentik Wangi are recommended to be used as superior varieties of waxy pigmented rice.

The Determination of Aromatic Character of Several Local Rice Varieties using Phenotypic Analysis and Molecular DNA

The pandan scent in aromatic rice has been known as the result of 8 bp deletions and 3 Single Nucleotide Polymorphisms (SNPS) in BADH2 gene, which produce non-functional betaine aldehyde dehydrogenase (BADH) enzyme. Several DNA markers for aromatic character based on mutation in BADH2 gene have been developed. In our experiment, we analysed the presence of aromatic character in four local rice variety such as Merah Wangi, Pendok, Genjah Arum, and Mentik Wangi Susu using KOH method and DNA molecular method using three DNA markers to detect mutation that responsible for the development of aromatic character. Phenotype analysis using KOH method showed that Merah Wangi, Genjah Arum, and Mentik Wangi Susu produce pandan scents. PCR analysis using Bradbury and Badex7-5, and RM223 markers showed the presence of BADH2 mutation in Merah Wangi and Mentik Wangi Susu, whereas Pendok and Genjah Arum did not show BADH2 mutation using those used three markers. Our results indicate that among four investigated local rice, only Merah Wangi and Mentik Wangi Susu are categorized as aromatic rice whereas Pendok and Genjah Arum are non-aromatic.Keywords: genetic analysis, aromatic rice, specific markers, genetic mutation.

Improving growth performance of rice cultivars IR64 and Mentik Wangi in drought stress with applying nano Zn

Increasing temperatures due to climate change caused irregular rainfall in some regions. Changes in rainfall initiate drought to increase every year and negatively impact vital human activities, including agricultural sectors. Drought stress is becoming increasingly important in agriculture because it affects changes in physiological function and leads to a severe decline in crop production. Nano Zn is one of the solutions for drought stress which has a role in enzyme activation, protein synthesis, and carbohydrate metabolism. This study aims to determine the effect of applying nano Zn on rice growth performance in drought stress conditions. The method used was a non-factorial Completely Randomized Design with analysis of variance followed by DMRT at 5% level. The levels of Zn concentration were 0; 0.5; 1; 1.5; 2 ppm Zn. Two varieties were used, ‘IR 64’ as a hybrid cultivar and ‘Mentik Wangi’ as a local cultivar in Indonesia. The variables observed were plant height (cm), number of tillers, number of leaves, biomass weight (g), and root dry weight (g). The result showed that applying nano Zn can increase rice growth due to drought stress on the various variables. The higher the nano Zn dosage, the higher plant height, number of tillers, number of leaves, biomass, and root dry weight by 3-6 %, 2-33 %, 6-23 %, 8-28 %, and 3-34 % respectively compared without Zn. These results can be considered a solution for environmental conditions affected by drought stress by paying attention to Zn concentration level.