A high shear rate can be applied to fluid near a membrane surface by rotating the membrane. This shear rate enables higher permeate flux and higher concentration operation when compared with a conventional cross-flow membrane since fouling and/or concentration polarization are reduced. The purpose of this study was to clarify the relationship between the fluid behavior and membrane separation characteristics of a rotating membrane surface when a latex aqueous solution was used. Due to the synergistic effect of particle removal by the centrifugal forces generated by the rotation of the membrane and the reduction in the thickness of the velocity boundary layer, membrane filtration of high-concentration slurry, which is difficult to dewater by the cross-flow method, is possible. The experimental data using an aqueous latex solution with a wide range of slurry concentrations and various membrane diameters are well correlated using a shear rate derived from the boundary layer theory. It is thus confirmed that the shear rate can be used as a design and operating parameter to define the membrane filtration characteristics.