Mechanism Of Membrane Damage Research Articles

Corannulene Amino Acid-Derived Water-Soluble Amphiphilic Buckybowls as Broad-Spectrum Membrane Targeting Antibacterial Agents.

To date, the use of corannulene has been restricted in the area of material science, but its application in biomedical research has yet to be established due to its nonsolubility in an aqueous environment and synthetic infeasibility. Herein, we detail the development of a new family of highly curved π-conjugated corannulene-containing unnatural α-amino acid (CAA) derivatives to overcome this challenge. These CAAs have been extended as novel constituents for the synthesis of corannulene-containing water-soluble cationic peptides (CCPs), which display inhibitory activity against broad-spectrum pathogenic bacteria along with drug-resistant bacteria via a membrane-damaging mechanism. Importantly, several of the synthesized peptides were found to be appreciably nonhemolytic against hRBCs and noncytotoxic against mammalian 3T3 cells. In vivo efficacy studies of the potent and least cytotoxic peptide 6a demonstrated clearance of bacteria from the spleen, liver, lung, and blood of mice infected with S. aureus ATCC 25923.

Efficacy and Mechanism of Antibiotic Resistance Gene Degradation and Cell Membrane Damage during Ultraviolet Advanced Oxidation Processes.

Combinations of UV with oxidants can initiate advanced oxidation processes (AOPs) and enhance bacterial inactivation. However, the effectiveness and mechanisms of UV-AOPs in damaging nucleic acids (e.g., antibiotic resistance genes (ARGs)) and cell integrity represent a knowledge gap. This study comprehensively compared ARG degradation and cell membrane damage under three different UV-AOPs. The extracellular ARG (eARG) removal efficiency followed the order of UV/chlorine > UV/H2O2 > UV/peracetic acid (PAA). Hydroxyl radical (•OH) and reactive chlorine species (RCS) largely contributed to eARG removal, while organic radicals made a minor contribution. For intracellular ARGs (iARGs), UV/H2O2 did not remove better than UV alone due to the scavenging of •OH by cell components, whereas UV/PAA provided a modest synergism, likely due to diffusion of PAA into cells and intracellular •OH generation. Comparatively, UV/chlorine achieved significant synergistic iARG removal, suggesting the critical role of the RCS in resisting cellular scavenging and inactivating ARGs. Additionally, flow cytometry analysis demonstrated that membrane damage was mainly attributed to chlorine oxidation, while the impacts of radicals, H2O2, and PAA were negligible. These results provide mechanistic insights into bacterial inactivation and fate of ARGs during UV-AOPs, and shed light on the suitability of quantitative polymerase chain reaction (qPCR) and flow cytometry in assessing disinfection performance.

Exploring membrane damage mechanisms of heterogeneous tau and amylin oligomers in Alzheimer's using MD simulations

Exploring membrane damage mechanisms of heterogeneous tau and amylin oligomers in Alzheimer's using MD simulations

Antimicrobial activity and mechanism of anti-MRSA of phloroglucinol derivatives.

In previous studies, authors have completed the total synthesis of several phloroglucinol natural products and synthesized a series of their derivatives, which were tested with good biological activities. To discover anti-MRSA lead compound and study their mechanism of action. Phloroglucinol derivatives were tested to investigate their activities against several gram-positive strains including Methicillin-resistant Staphylococcus aureus (MRSA). The mechanism study was conducted by determining extracellular potassium ion concentration, intracellular NADPH oxidase content, SOD activity, ROS amount in MRSA and MRSA survival rate under A5 treatment. The in vitro cytotoxicity test of A5 was conducted. The activity of monocyclic compounds was stronger than that of bicyclic compounds, and compound A5 showed the best MIC value of 0.98μg/mL and MBC value of 1.95μg/mL, which were 4-8 times lower than that of vancomycin. The mechanism study of A5 showed that it achieved anti-MRSA effect through membrane damage, which is proved by increased concentration of extracellular potassium ion after A5 treatment. Another possible mechanism is the over ROS production induced cell death, which is suggested by observed alternation of several reactive oxygen species (ROS) related indicators including NADPH concentration, superoxide dismutase (SOD) activity, ROS content and bacterial survival rate after A5 treatment. The cytotoxicity results in vitro showed that A5 was basically non-toxic to cells. Acylphloroglucinol derivative A5 showed good anti-MRSA activity, possibly via membrane damage and ROS-mediated oxidative stress mechanism. It deserves further exploration to be a potential lead for the development of new anti-MRSA agent.

Effect of chlorogenic acid grafted chitosan on microbiological compositions of sea bass (Lateolabrax japonicus) fillets: Dominant spoilage bacteria, inhibition activity and membrane damage mechanisms

This study investigated the effect of chlorogenic acid grafted chitosan (CS-g-CA) on the microbiota composition of sea bass (Lateolabrax japonicus), isolated and identified the specific spoilage organisms (SSOs) in the late stage of refrigerated fillets and evaluation of their spoilage potential. Moreover, antibacterial activity and membrane damage mechanism of CS-g-CA against spoilage bacteria was also investigated. Illumina-MiSeq high throughput sequencing results showed that CS-g-CA retarded the growth of Pseudomonas spp., which largely contributed to delaying the quality degradation of sea bass during storage. Then nine spoilage bacteria were isolated and identified from the fillets at the end of storage and inoculated into sterile fish fillets to determine their spoilage capacity. Results showed that fish fillets inoculated with spoilage bacteria exhibited a significant increase in TVB-N, TBA and putrescine content and decreased sensory quality during storage. Subsequently, the inhibitory activity of CS-g-CA against spoilage bacteria was investigated and strains that were more sensitive to the CS-g-CA with a strong spoilage capacity were selected for the study of the inhibition mechanism. Results suggested that CS-g-CA had strong inhibitory activity and led to bacterial death through the mechanism of membrane damage. Overall, this study analyzed the effect of CS-g-CA on the preservation of fish fillets from a microbiological point of view to provide a reference for the anti-bacterial preservation of aquatic products.

Insight into the antibacterial activity and membrane-damage mechanism of pyrrole-2-carboxylic acid against Listeria monocytogenes

Natural antimicrobials have potential to suppress the growth of foodborne pathogens. In this work, the antibacterial property and membrane-disruptive mechanism of pyrrole-2-carboxylic acid (PCA), against Listeria monocytogenes were elucidated. Leakage of cell constituents (K+ and nucleotide) and flow cytometric analysis indicated that PCA caused a significant change in the permeability and integrity of cell membrane of L. monocytogenes. Meanwhile, remarkable morphological alterations and membrane hyperpolarization of L. monocytogenes validated the disruptive ability of PCA on cell membrane. Additionally, PCA could interact with membrane protein, and cause changes in the conformation of protein. Untargeted lipidomics revealed that PCA triggered alternations in the composition and architecture of the membrane lipids mainly involved in glycerophospholipid and glycerolipid metabolism, thus leading to cell membrane dysfunction. Furthermore, PCA at concentration of 0.75 mg/mL could inhibit the contamination of L. monocytogenes in lettuce stem and beef, with a decrease of 2.4 log CFU/cm2 (P ＜ 0.05) for lettuce stem and 1.8 log CFU/cm2 (P ＜ 0.05) for beef after 10 days of storage. This work illuminates the key targets of PCA against the cell membrane of L. monocytogenes, facilitating the application of natural organic acids as candidate food preservatives in the food industry.

Oxydextran-based photodynamic antibacterial nanoplatform with broad-Spectrum antibacterial activity

The compounding of polysaccharide macromolecules and antibacterial agents always has been the preferred strategy to prepare antibacterial products, attracting increasing interest. Herein, a novel acid-responsive oxidized dextran-based nanoplatform (OTP NP) has been fabricated for photodynamic antibacterial therapy by combing photosensitizer monoaminoporphyrin (TPP-NH2) with oxidized dextran (ODex) via the Schiff Base reaction. OTP NP of about 100 nm is composed of an inner hydrophobic core of 30 nm and peripheral polysaccharide macromolecules. The OTP NP killed 99.9 % of E. coli and S. aureus within 1.5 light cycles at a concentration of 200 μg/mL. Concurrently, OTP NP exhibited excellent cytocompatibility at a concentration of 1 mg/mL (about 5 folds bactericidal concentration). Particularly, except for the recognized antibacterial mechanism of photodynamic therapy, a novel mechanism of bacterial membrane damage was discovered: the bacterial cell membrane was peeled off and formed spherical particles that aggregated around the bacteria to accelerate bacterial apoptosis under the combined action of ROS and nanomaterials. Moreover, the slightly soluble drug levofloxacin (Lev) as a model drug was loaded into OTP NP to test its carrier function, providing a practicable strategy to design multifunctional polysaccharide-based photodynamic antibacterial materials.

Damage Effect of Amorphous Carbon Black Nanoparticle Aggregates on Model Phospholipid Membranes: Surface Charge, Exposure Concentration and Time Dependence.

Commercial nano-scale carbon blacks (CB) are being harnessed widely and may impose potentially hazardous effects because of their unique properties, especially if they have been modified to grow reactive functional groups on their surface. Cytotoxicity of CB has been well studied but the membrane damage mechanisms and role of surface modification are still open to debate. Negatively and positively charged giant unilamellar vesicles (GUVs) were prepared using three lipids as model cell membranes to examine the mechanistic damage of CB and MCB (modified by acidic potassium permanganate) aggregates. Optical images showed that both anionic CB and MCB disrupted the positively charged but not the negatively charged GUVs. This disruption deteriorated with the rise and extension of exposure concentration and time. Lipids extraction caused by CBNs (CB and MCB together are called CBNs) was found. MCB caused more severe disruption than CB. MCB was enveloped into vesicles through an endocytosis-like process at 120 mg/L. MCB mediated the gelation of GUVs, perhaps through C-O-P bonding bridges. The lower hydrodynamic diameter and more negative charges may have been responsible for the distinction effect of MCB over CB. The adhesion and bonding of CBNs to the membrane were favored by electrostatic interaction and the practical application of CBNs warrants more attention.

Surface-induced protein folding of human islet amyloid polypeptide on neuronal membrane domains.

Aggregated human islet amyloid polypeptide (hIAPP) has been associated with the development of type II diabetes. However, recent evidence suggests that disordered hIAPP aggregates, or hIAPP oligomers, may also bind to the neurons in the brain causing membrane damage and thereby contributing to the cognitive decline of people with Alzheimer's Disease (AD). At present, the detailed membrane damage mechanisms of disordered hIAPP oligomers on neuronal membranes are unknown. Using both coarse-grained (CG) and all-atom (AA) molecular dynamics (MD) simulations, binding of disordered hIAPP oligomers (monomer, dimer, and tetramer) to phase-separated lipid bilayers (raft membranes), a model of neuronal plasma membrane domains, was investigated. We observed membrane binding of hIAPP oligomers to raft membranes containing mixed liquid-ordered (Lo) and liquid-disordered (Ld) boundary or Lod domains on both lipid leaflets of the lipid bilayer. However, much stronger binding to ganglioside (GM1) clusters embedded in the Lo domains and phosphatidylserine (PS) clusters embedded in the Lo and Lod domains, located in only one leaflet of the lipid bilayer, was evident. All oligomers binding events occurred within 10 μs. Our results suggest that the Lod domains represent the non-specific targets in both lipid leaflets, while the PS-clusters and GM1-clusters represent the specific targets in the inner lipid leaflet and outer lipid leaflet, respectively, of the plasma membranes of neurons. After a CG-to-AA resolution transformation and a 200 ns-long AA MD simulation, surface-induced protein folding, particularly the formation of localized inter-chain and intra-chain beta-sheets, in several hIAPP-raft complexes, especially those involving Lod domains, was evident. We propose that these localized beta-sheets may act as nucleation seeds to attract nearby hIAPP or non-hIAPP oligomers to form larger surface-bound amyloid aggregates and create membrane damage to the neurons that leads to the pathogenesis of AD.

Applying machine learning to investigate lipid domain formation behaviors in the presence of amyloidogenic proteins.

Cell membranes are not homogeneous but contain lipid nanodomains distributed on both leaflets of the lipid bilayers. Using microsecond molecular dynamics (MD) simulations, we have simulated phase-separated lipid bilayers (raft membranes) containing lipid nanodomains that mimic the heterogeneous structures of neuronal plasma membranes. Our recent studies indicate that these lipid nanodomains, e.g., the mixed liquid-ordered (Lo) and liquid-disordered (Ld) boundaries or Lod and ganglioside-cluster, represent non-specific and specific membrane damage targets of amyloidogenic protein, e.g., tau, aggregates (oligomers), in raft membranes. However, a robust computational tool to classify lipids into these lipid nanodomains and quantitate the spatial and temporal evolutions of the domain structures in the presence of amyloidogenic oligomers is not available. In this study, we have modified two unsupervised Machine Learning (ML) algorithms, Non-negative Matrix Factorization (NMFk) and K-Mean Clustering, to investigate lipid domain formation behaviors. These algorithms are currently used in mutation analysis of cancer genomes and feature extractions. In this work, NMFk was used to decompose our time-resolved lipid-lipid proximity data matrix into two latent “signature” and “activity” matrices, which contain a set of probability configurations and the time-dependent activity of those configurations, respectively, of the lipids participating in each nanodomain, e.g., Lo and Lod. On the other hand, by implementing additional spatial information to the dataset used in NMFk, the K-Mean Clustering sorts lipids into nanodomains and determines the latent temporal and spatial evolutions of each domain. Hence, by combining both ML algorithms, this study provides new insights into understanding protein-induced membrane damage mechanisms of amyloidogenic oligomers associated with lipid domain disruption behaviors that may contribute to the pathogenesis of Alzheimer's. (Supported by NSF [OAC 153159] and Welch Foundation [W-2057-2021-327].)

Mechanism of membrane damage to Shigella sonnei by linalool from plant essential oils: A driver of oxidative stress

This work explored the antibacterial mechanism of linalool on Shigella sonnei (S. sonnei) based on membrane damage driven by oxidative stress. The results revealed that linalool had excellent inhibition towards S. sonnei under a concentration of 1.5 mL/L. Furthermore, cell structure was disrupted with massive leakage of K+ and Mg2+ with linalool according to scanning electron microscopy, zeta potential and probes staining of fluorescein diacetate and Rhodamine 123 combined with fluorescence spectroscopy. Changes in membrane protein conformation were also monitored as a result of fluorescence spectroscopy when exposed to linalool. In addition, oxidative stress was appeared after exposure to linalool due to the increase of malondialdehyde, hydrogen peroxide, superoxide anion and oxidized glutathione, and the decrease of reduced glutathione. The activity of defense enzyme including superoxide dismutase, catalase and peroxidase, and antioxidant enzyme including glutathione reductase and glutathione S-transferase was also inhibited. In all, oxidative stress-driven membrane damage was considered as the main mechanism by which linalool inhibited S. sonnei. Our findings provide theoretical support for linalool as a promising natural antibacterial agent against S. sonnei contamination.

Membrane Integrity Assay in Ferroptosis.

Ferroptosis is a form of regulated cell death that relies on the accumulation of intracellular iron and subsequent oxidative stress. Ferroptotic cell death is characterized by uncontrolled lipid peroxidation, which leads to plasma membrane damage and rupture. The loss of plasma membrane integrity results in the release of intracellular components, including damage-associated molecular patterns, and can propagate death between cells in a synchronized manner. Understanding the mechanisms of ferroptotic membrane damage is crucial to comprehending this form of cell death. This chapter provides a summary of techniques for detecting plasma membrane integrity in ferroptosis, including transmission electron microscopy analysis, flow cytometry analysis, and assessments of oxidoreductase-mediated membrane damage.

Disruption of plant plasma membrane by Nep1-like proteins in pathogen-plant interactions.

Lipid membrane destruction by microbial pore-forming toxins (PFTs) is a ubiquitous mechanism of damage to animal cells, but is less prominent in plants. Nep1-like proteins (NLPs) secreted by phytopathogens that cause devastating crop diseases, such as potato late blight, represent the only family of microbial PFTs that effectively damage plant cells by disrupting the integrity of the plant plasma membrane. Recent research has elucidated the molecular mechanism of NLP-mediated membrane damage, which is unique among microbial PFTs and highly adapted to the plant membrane environment. In this review, we cover recent insight into how NLP cytolysins damage plant membranes and cause cell death.

γ-Cyclodextrin-Encapsulated Cinnamaldehyde for Citrus Preservation and Its Potential Mechanisms against Penicillium digitatum.

In this study, a γ-cyclodextrin-cinnamaldehyde inclusion compound (γ-CDCL) was prepared to control green mold caused by Penicillium digitatum (P. digitatum) in citrus. The results showed that the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of γ-CDCL against the mycelial growth of P. digitatum were 2.0 g L-1 and 4.0 g L-1, respectively. Simultaneously, eight × MFC γ-CDCL could effectively reduce the incidence of green mold in citrus fruit without impairment of the fruit qualities, meanwhile, eight × MFC γ-CDCL was comparable to Prochloraz in controlling fruit under natural storage conditions. The structure of γ-CDCL was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform-infrared spectroscopy (FT-IR), and nuclear magnetic resonance (NMR) analyses. Results showed that the successful preparation of γ-CDCL was due to the spatial interaction between H-4,8 of cinnamaldehyde and H-5' of γ-cyclodextrin. Meanwhile, the cell membrane permeability of P. digitatum was impaired by γ-CDCL through massive accumulation of reactive oxygen species, whereas the cell wall integrity was barely affected. These results indicated that γ-CDCL might inhibit the growth of P. digitatum through a membrane damage mechanism and it is a promising alternative to chemical fungicides in controlling the post-harvest citrus decay.

Membrane damage mechanism of protocatechualdehyde against Micrococcus luteus and its effect on pork quality characteristics

This study investigated the mechanism of membrane damage by protocatechualdehyde (PCA) against Micrococcus luteus and assessed effects of PCA on the sensory and physicochemical properties of pork. The mechanism of PCA inhibition on M. luteus was studied by determining the minimum inhibitory concentration (MIC) based on membrane potential, intracellular ATP concentration, intracellular pH, confocal laser scanning microscopy (CLSM), and field emission gun scanning electron microscopy (FEG-SEM). The results showed that the MIC of PCA against M. luteus was 1.25 mg/mL. Hyperpolarization of the bacterial cell membrane, a decrease in the intracellular ATP concentration, and intracellular pH indicated that PCA damaged the cell membrane of M. luteus. FEG-SEM observation revealed that PCA could cause surface collapse, cell membrane rupture, and content outflow of M. luteus. Additionally, PCA was found to inhibit increases in the total number of colonies, the thiobarbituric acid reactive substances (TBARS) value growth rate, and moisture mobility in raw pork. Additionally, it improved the color and texture of raw pork, all of which effectively prolonged its shelf life. This study will encourage the application of PCA as a natural antibacterial agent in the food industry.

Cooperative Membrane Damage as a Mechanism for Pentamidine-Antibiotic Mutual Sensitization.

Most Gram-positive-selective antibiotics have low activity against Gram-negative bacteria due to the presence of an outer membrane barrier. There is, therefore, interest in developing combination therapies that can penetrate the outer membrane (OM) with known antibiotics coupled with membrane-active sensitizing adjuvants. However, two unanswered questions hinder the development of such combination therapies: the sensitization spectrum of the sensitizer and the mechanism of antibiotic-sensitizer mutual potentiation. Here, with pentamidine as an example, we screened a library of 170 FDA-approved antibiotics in combination with pentamidine, a compound known to disturb the OM of Gram-negative bacteria. We found that four antibiotics, minocycline, linezolid, valnemulin, and nadifloxacin, displaced enhanced activity in combination with pentamidine against several multidrug-resistant Gram-negative bacteria. Through a descriptor-based structural-activity analysis and multiple cell-based biochemical assays, we found that hydrophobicity, partial charge, rigidity, and surface rugosity were key factors that affected sensitization via a cooperative membrane damage mechanism in which lipopolysaccharides and phospholipids were identified as sites of synergy. Finally, in vitro experiments showed that the linezolid-pentamidine combination slowed the generation of drug resistance, and there was also potent activity in in vivo experiments. Overall, our results highlight the importance of the physicochemical properties of antibiotics and cooperative membrane damage for synergistic pentamidine-antibiotic drug combinations.

The ester derivatives of ferulic acid exhibit strong inhibitory effect on the growth of Alternaria alternata in vitro and in vivo

Alternaria alternata is the principal pathogenic fungus causing postharvest black spot disease of Korla pear, leading to enormous economic losses. This study aimed to explore the antifungal activity of ferulic acid (FA) and its two simple esterified derivatives, methyl ferulate (MF) and ethyl ferulate (EF), against A. alternata in vitro and in vivo, and to reveal the underlying inhibitory mechanisms. The results showed that MF and EF exhibited stronger inhibitory effects than FA on mycelial growth, spore germination and germ tube elongation of A. alternata in vitro, and on reducing the development of black spot disease in Korla pear fruit. Scanning electron microscopy images indicated that the surface morphology of A. alternata hyphae treated with FA, MF, and EF presented irreversible deformation, and transmission electron microscopy observation further demonstrated that MF and EF caused more serious damage to the cell membranes, cytoplasm, and organelles of A. alternata than FA, which impeded the normal growth of A. alternata. Notably, EF was slightly better than MF in inhibiting spore germination and damaging cell ultrastructure of A. alternata. In addition, propidium iodide staining suggested that FA, MF, and EF treatments damaged the plasma membrane integrity of A. alternata spores. At the same conditions, MF and EF treatments induced more leakage of intercellular electrolytes, nucleic acids and proteins of A. alternata than FA, indicating the increase of cell membrane permeability. Taken together, all these results evidenced that MF and EF exerted stronger antifungal activity than FA against A. alternata in vitro and in vivo through membrane damage mechanism, therefore they can be used as promising green substitutes to control postharvest black spot disease on Korla pear fruit.

Exercise-Induced Muscle Damage and Protein Intake: A Bibliometric and Visual Analysis.

Numerous studies have covered exercise-induced muscle damage (EIMD) topics, ranging from nutritional strategies to recovery methods, but few attempts have adequately explored and analyzed large volumes of scientific output. The purpose of this study was to assess the scientific output and research activity regarding EIMD and protein intake by conducting a bibliometric and visual analysis. Relevant publications from 1975–2022 were retrieved from the Web of Science Core Collection database. Quantitative and qualitative variables were collected, including the number of publications and citations, H-indexes, journals of citation reports, co-authorship, co-citation, and the co-occurrence of keywords. There were 351 total publications, with the number of annual publications steadily increasing. The United States has the highest total number of publications (26.21% of total publications, centrality 0.44). Institutional cooperation is mostly geographically limited, with few transnational cooperation links. EIMD and protein intake research is concentrated in high-quality journals in the disciplines of Sport Science, Physiology, Nutrition, and Biochemistry & Molecular Biology. The top ten journals in the number of publications are mostly high-quality printed journals, and the top ten journals in centrality have an average impact factor of 13.845. The findings of the co-citation clusters and major keyword co-occurrence reveal that the most discussed research topics are “exercise mode”, “nutritional strategies”, “beneficial outcomes”, and “proposed mechanisms”. Finally, we identified the following research frontiers and research directions: developing a comprehensive understanding of new exercise or training models, nutritional strategies, and recovery techniques to alleviate EIMD symptoms and accelerate recovery; applying the concept of hormesis in EIMD to induce muscle hypertrophy; and investigating the underlying mechanisms of muscle fiber and membrane damage.

Evaluation of the membrane damage mechanism of chlorogenic acid against Bacillus cereus and Micrococcus luteus a simulation study on antibacterial growth in food

AbstractThis study aimed to examine the mechanism of membrane damage by chlorogenic acid (CA) on Bacillus cereus and Micrococcus luteus and to apply it to milk and pork to evaluate whether CA could prolong their shelf life. A broth microdilution method was used to determine the minimum inhibitory concentration (MIC) of CA on B. cereus and M. luteus. In addition, the physiological changes and morphology of B. cereus and M. luteus after CA treatment were explored using membrane potential, intracellular ATP concentration, confocal laser scanning microscopy, and field emission gun scanning electron microscopy (FEG‐SEM). The results showed that the MIC value of CA on both B. cereus and M. luteus was 2.5 mg/ml. The abnormal changes in membrane potential and the decrease of intracellular ATP concentration indicated that CA affected the membrane integrity of B. cereus and M. luteus. Observation by FEG‐SEM showed that CA ruptured the cell membrane of B. cereus and M. luteus, causing the contents to flow out, and giving the cells a concave morphology. Finally, growth inhibition models of CA on B. cereus in skim milk and M. luteus in pork were developed using response surface methodology. The results demonstrated that 2.5 mg/ml of CA significantly inhibited the growth of B. cereus in skim milk and of M. luteus in pork. Therefore, CA can be used as a natural antimicrobial agent to prolong the shelf life of milk and pork.

Evaluation of gallic acid on membrane damage of Yersinia enterocolitica and its application as a food preservative in pork

This study was aimed to examine the membrane damage mechanism of gallic acid (GA) on Yersinia enterocolitica BNCC 108930, and to explore whether GA can prolong the shelf life of pork. The minimal inhibitory concentration (MIC) of GA against Y. enterocolitica was determined by adopting the broth microdilution method. Second, an investigation was conducted on the morphological and physiological variations of Y. enterocolitica after the GA treatment. Finally, a response surface approach was used to establish the growth inhibition model of GA against Y. enterocolitica in pork. The MIC of GA against Yersinia enterocolitica BNCC 108930 was 2.5 mg/mL. GA affects the membrane integrity of Y. enterocolitica, as demonstrated by a significant decrease in intracellular ATP and pH. The results showed that the number of Y. enterocolitica in pork meat containing 5 mg/g of GA decreased by 2 logarithmic cycles during storage at 4 °C for 3 days. According to the obtained findings, GA could be used as a food preservative to prolong the shelf life of pork.