Members Of Branch Research Articles

Analysis of NRAMP genes in the Triticeae reveals that TaNRAMP5 positively regulates cadmium (Cd) tolerance in wheat (Triticum aestivum)

Natural Resistance-Associated Macrophage Protein (NRAMP), a class of metal transporter proteins widely distributed in plants, is mainly involved in the uptake and transport by plants of metal ions, such as iron, manganese and cadmium. The current study is the first to fully investigate the Triticum aestivum (T. aestivum) NRAMP gene family. 33 NRAMP members were identified from the entire T. aestivum genome and classified into three main groups based on related genes found in five other species. Among the TaNRAMP genes, the exon-intron structure and motif composition exhibited significant similarity among members of the same evolutionary branch of the phylogenetic tree. Based on RNA-seq and qRT-PCR analyses, we identified the expression patterns of the TaNRAMP genes in different tissues and under various stress conditions. TaNRAMP genes expression were responsive to induction by cadmium (Cd). Overexpression of the TaNRAMP5 gene enhanced wheat and tobacco tolerance to Cd toxicity. Additionally, the TaNRAMP5 protein physically interacted with protein phosphatase 2A (PP2A) in yeast cells. This study provides a valuable reference point for further investigations into the functional and molecular mechanisms of the NRAMP gene family.

SI TUTI-BASED GIRLS' SCHOOL (ANTI-STUNTING LITERACY)

Background: Stunting is a major public health issue in Indonesia, particularly in rural areas where limited access to health information exacerbates the problem. Empowering women through health literacy is essential in addressing stunting and related health risks. Specific Background: In Gedangan Village, Pasuruan Regency, high rates of stunting, poor maternal nutrition, and the prevalence of early marriage have been identified as key challenges. Existing interventions have lacked comprehensive, community-based approaches to stunting prevention. Knowledge Gap: There is a need for scalable models that combine education, digital tools, and community involvement to effectively address stunting at the local level. Aims: This study aimed to evaluate the impact of the SI TUTI (Anti-Stunting Literacy) Based Women's School Program, designed to empower women through education and digital resources to reduce stunting rates. Results: The program involved PKK Cadres, Posyandu, and Aisyiyah Branch Members, with the SI TUTI mini book and ASTUTI application as key tools. Post-training assessments showed a 30% increase in participants' understanding of stunting, with a 95% satisfaction rate regarding educational materials and media. The use of digital tools facilitated improved access to health information. Novelty: This program integrates traditional education methods with digital innovations, offering a replicable model that addresses stunting at the grassroots level, enhancing community engagement through localized content and accessible health literacy tools. Implications: The program demonstrates potential for broader application in similar rural contexts, contributing to the achievement of the 2030 Sustainable Development Goals related to public health and well-being. Future efforts will focus on program expansion and continuous evaluation to ensure long-term sustainability and effectiveness in stunting prevention.

Genome-wide analysis of the WOX gene family and function exploration of RhWOX331 in rose (R. 'The Fairy').

WOXs are a class of plant-specific transcription factors that play key roles in plant growth and stress responses. However, the mechanism by which WOXs influence adventitious root development in Rosa hybrida remains unclear. In this study, RcWOX gene family in rose was identified and phylogenetically analyzed using bioinformatics analysis. A total of 381 RcWOX gene members were localized on seven chromosomes except of nine members. The main cis-acting elements involved in hormonal, light, developmental, and abiotic stress responses were identified in the promoters of RcWOX genes, suggesting their regulation by these signals. Nine RhWOX genes had significant different expression during rooting process of rose. RhWOX331, RhWOX308, RhWOX318 were positive with the formation of rose roots. RhWOX331 was positively involved in the formation of adventitious root primordia, which gene coding a transcription factor localized in the nucleus. The HOX conserved domain in the protein contributed to the self-activating activity of RhWOX331. We obtained genetically modified Arabidopsis to validate the function of RhWOX331. Overexpression of RhWOX331 gene alleviated the inhibition of root length of A. thaliana primary roots by high concentration of IBA and NPA, and significantly increased the number of lateral roots on the primary roots, as well as the height of A. thaliana plants. Additionally, RhWOX331 promoted adventitious root formation in A. thaliana and mitigated hormonal inhibition by exogenous 6-BA, NPA, and GA3. The RhWOX331 promoter contained cis-acting elements such as ABRE, Box 4 and CGTCA-motif et.al. GUS activity analysis showed that the gene acted at the cotyledon attachment site. Taken together, these studies identified a significant expansion of the RcWOX gene family, inferred roles of certain branch members in adventitious root formation, elucidated the function of RhWOX331 in adventitious root initiation, and laid the foundation for further research on the function of WOX gene family in roses.

Lazerckerite, Ag3.75Pb4.5(Sb7.75Bi4)S24, from Kutná Hora, Czech Republic: a new Sb–Bi member of the andorite branch of the lillianite homologous series

Abstract. Lazerckerite, ideally Ag3.75Pb4.5(Sb7.75Bi4)S24, is a new mineral species found in medieval mine dumps of the historic Ag–Pb–Zn Kutná Hora ore district, Czech Republic. The mineral is associated with other Sb–Bi lillianite homologues (terrywallaceite, gustavite, holubite) and Ag,Bi-bearing galena, most frequently as grain aggregates and replacement rims of earlier Ag–Pb–Bi minerals, growing together in aggregates of up to 0.6×0.3 mm. Lazerckerite is opaque, is steel grey in colour, and has a metallic lustre; the calculated density is 5.920 g cm−3. In reflected light lazerckerite is greyish white, and bireflectance and pleochroism are weak with grey tints. Anisotropy is weak to moderate with grey to bluish-grey rotation tints. Internal reflections are not observed. Electron microprobe analyses yielded the empirical formula, based on 44 apfu, (Ag3.61Cu0.04)Σ3.65(Pb4.55Fe0.01Cd0.01)Σ4.57(Sb7.87Bi3.75)Σ11.62(S24.15Se0.01)Σ24.16. Its unit cell parameters are a=13.2083(9), b=19.4595(8), c=8.4048(13), β=90.032(7)°, V=2160.3(4) Å3, space group P21/c, and Z=2. The structure of lazerckerite contains two Pb sites (Pb1 and Pb2) in bicapped trigonal prismatic coordination, 8 independent octahedral sites, and 13 distinct sulfur positions. Four of the octahedral sites are mixed (Sb,Bi) and (Bi,Sb) sites, one is a mixed (Ag,Bi) site, and one is a mixed (Sb,Pb) site. The new mineral belongs to the andorite branch of the lillianite homologous series with N=4 and is a new addition to the group of Sb–Bi mixed members of the series. Lazerckerite is defined as a lillianite homologue with the three following requirements: N=4, L (Ag++ (Bi3+, Sb3+) ↔ 2 Pb2+ substitution) ≈ 90 %–95 %, and approximately one-third of atom percentage of antimony is replaced by bismuth (Bi/(Bi+Sb) ≈ 0.30–0.38). The new mineral has been approved by the Commission on New Minerals, Nomenclature and Classification (CNMNC) of the International Mineralogical Association (IMA 2022-113) and named after Lazarus Ercker, the supreme Royal Bergmeister of the Kingdom of Bohemia and the Master of Prague Mint.

INTEGRATED ANTHROPOLOGICAL RESEARCH ON KORKU TRIBES IN 2020 (INDIA, MAHARASHTRA). PRELIMINARY RESULTS

Introduction. In February 2020, the third season of the Russian-Indian Anthropological expedition was conducted in Maharashtra. The field work was coordinated by Paleoethnology Research Center (Russia) and Department of Anthropology of Savitribai Phule Pune University (India). The goal was to examine Korku people, who are members of the Mundo-speaking branch of the Austroasiatic family. Data and methods. A total of 293 people were examined from three different Amravati district locations. The sociological questionnaire indicated that 285 individuals were members of the Korku, of which 196 individuals were children and teenagers (95 male, 101 female) and 89 individuals were older than 17 years (58 male, 31 female). Following the traditions of the Russian anthropological school, the study of Korku was conducted using as many morphological techniques as possible, including the collection of data on somatometry, cephalometry, cephaloscopy, dental anthropology, dermatoglyphics, and anthropological photo. All data was collected according to the law protecting personal data and the principles of bioethics. Results and discussion. Korku people tend to have dark pigmentation of the eyes, hair and skin, and soft, wavy hair. Males are orthognathic in the vertical profile of the face, females are mesognathic. Horizontal profiling in both sexes is characterized by average values. The lateral profile of the nasal bridge is flattened, the eye gap is of medium width and has horizontal orientation, while epicanthus is rare. According to the head index, males are mesocephalic with a tendency to subbrachycephaly, the proportions of the face and nose are characterized by average values. Women are also mesocephalic, relatively leptorineic. According to somatometric indicators, males have an average body length (164.4 cm). According to the proportions of the body, males and females are brachymorphic. The body length and chest circumference of children increases smoothly with age. The same growth curve is typical for body weight with a slight jump in the transition from 10–11 y.o. to 12–14 y.o. Composite portraits of Korku males and females were compiled in three standard norms. Dermatoglyphics and dental anthropology data are still being analyzed. Conclusion. The Russian-Indian Anthropological Expedition examined the Korku people using an integrated anthropological approach during the 2020 field season. The Korku's somatometric, cephalometric, and cephaloscopic characteristics were acquired and presented for the first time.

A summary of the main themes and findings presented at the ASM Intermountain Branch meeting (2024).

The annual meeting for the Intermountain Branch was held in April 2024 on the campus of Brigham Young University. There were 127 branch members from Utah, Idaho, and Nevada who attended the meeting and were composed of undergraduate students, graduate or medical students, and faculty. This report highlights the diversity of, and the emerging trends in, the research conducted by American Society for Microbiology members in the Intermountain Branch.

Faktor Determinan Keputusan Menabung dengan Minat Sebagai Variabel Intervening

This study aims to determine the effect of religiosity, knowledge, promotion, location and service quality variables on saving decisions. The religiosity of the people who only view Islamic financial institutions is the same as conventional financial institutions. The customers' desire to save can be hindered by remote locations, activities and busyness. In addition, the low level of public knowledge about Islamic financial institutions influences the decision to become a customer of an Islamic financial institution. The population in this study were members of the Ben Sejahtera BMT Rawalo Banyumas branch. The number of samples in this study were 92 respondents. The data obtained was then analyzed using the PLS (Partial Least Square) analysis technique through the SmartPLS 4.0 software. The results showed that knowledge, promotion, and location factors had no effect on members' interest in saving at Islamic financial institutions. Meanwhile, the factors of religiosity and service quality are the variables that most influence the interest of traders in making saving decisions at Islamic financial institutions.

Penguatan Kesadaran Hidup Berkoperasi dalam Meningkatkan Partisipasi Anggota pada KSP Kopdit Pintu Air Cabang Maumere

This research aims to find out how to strengthen awareness of cooperative life in increasing member participation in KSP Kopdit Pintu Air Maumere Branch. To identify problems, this research uses qualitative research. The data collection techniques use observation, interviews and documentation. It can be concluded that the factors that influence the decline in member participation are caused by members' lack of knowledge in cooperative life, economic constraints, and fraud or abuse. Efforts to increase member participation include providing education and training, open communication, and participation in decision making. Keywords : Participation of Members of KSP Kopdit Pintu Air Maumere Branch, Strengthening Awareness of Cooperative Life

FOSTERING RELIGIOUS VALUES FOR MEMBERS OF GP. ANSOR RANTING KEBOJONGAN VILLAGE, COMAL PEMALANG DISTRICT

The purpose of this research is to analyze the development of religious values of Ansor Youth Movement (GP Ansor) members. One of the religious-based community organizations capable of realizing the nation's progress is the Ansor Youth Movement (GP Ansor). How much and how far religious values can influence and shape a person's character depends on how much religious values are embedded in him. This research includes field research, with a qualitative approach, which is research that produces analytical procedures that do not use statistical analysis. The data obtained were then analyzed using qualitative descriptive analysis techniques. The results of this study show that religious values are fostered by members of GP Ansor Kebojongan Branch by giving good advice, teaching about morals from the book of Taitsirul Kholaq and Akhlakul Banin, as well as teaching the book of Aqidatul Awwam and holding additional activities such as maulidan activities, grave pilgrimage prayers, and others. While the supporting factor is the development of religious values for members of GP. Ansor Ranting Kebojongan, Comal Sub-District, Pemalang Regency is the recitation of the book of Taisul Kholaq, and support from the community, while the inhibiting factors are inadequate infrastructure and the busyness of the members.

Imperfect legislative agreements and judicial policymaking. A theoretical inquiry

ABSTRACT This article examines how imperfect legislative agreements offer an opportunity for higher courts’ intervention in policymaking. In concrete terms, it explains that lawmakers in certain situations avoid resolving apparent conflicts of interest due to high transaction costs in the legislative process, maintaining statutory criteria considered transparently inefficient or by simply developing regulatory arrangements with a significant degree of indeterminacy. Employing a simplified model of rational choice behaviour, the article asserts that those scenarios constitute an opportunity for strategic involvement of the higher courts in crafting public policy. As an unintended consequence of the imperfect legislative agreements, justices look at them as an indicator that members of the other branches of government have reached weak political compromises or are unable to impose their policy preferences. Therefore, justices can exercise discretion, foreseeing prospects to increase their authority while the risk of an overruling or the implementation of other retaliatory measures remains low.

Identification of the succinate-CoA ligase protein gene family reveals that TaSUCL1-1 positively regulate cadmium resistance in wheat

The Succinate-CoA ligase (SUCL1) gene family is involved in energy metabolism, phytohormone signaling, and plant growth, development, and tolerance to stress. This is the first study to analyze the SUCL1 gene family in wheat (Triticum aestivum). 17 TaSUCL1 genes were identified in the complete genome sequence and classified into five subfamilies based on related genes found in three other species. The 17 TaSUCL1 genes were unevenly distributed across 11 chromosomes, and the collinearity of these genes was further investigated. Through using real-time qPCR (RT-qPCR) analysis, we identified the expression patterns of the TaSUCL1 genes under various tissues and different heavy metal stress conditions. The functions of selected TaSUCL1-1 gene were investigated by RNA interference (RNAi). This study provided a comprehensive analysis of the TaSUCL1 gene family. Within the TaSUCL1 genes, the exon-intron structure and motif composition exhibited significant similarity among members of the same evolutionary branch. Homology analysis and phylogenetic comparison of the SUCL1 genes in different plants offered valuable insights for studying the evolutionary characteristics of the SUCL1 genes. The expression levels of the TaSUCL1 genes in different tissues and under various metal stress conditions reveal its important role in plant growth and development. Gene function analysis demonstrated that TaSUCL1-1 silenced wheat plants exhibited a decrease in the total cadmium (Cd) concentrations and gene expression levels compared to the wild type (WT). Additionally, TaSUCL1-1 belonging to class c physically interacts with the β-amylase protein TaBMY1 as verified by yeast two-hybridization. This research provides a useful resource for further study of the function and molecular genetic mechanism of the SUCL1 gene family members.

Characterization and expression profiles of the ZmLBD gene family in Zea mays.

The Lateral Organ Boundaries Domain (LBD) gene family is a family of plant-specific transcription factors (TFs) that are widely involved in processes such as lateral organ formation, stress response, and nutrient metabolism. However, the function of LBD genes in maize remains poorly understood. In this study, a total of 49 ZmLBD genes were identified at the genome-wide level of maize, they were classified into nine branches based on phylogenetic relationships, and all of them were predicted to be nuclear localized. The 49 ZmLBD genes formed eight pairs of segmental duplicates, and members of the same branches' members had similar gene structure and conserved motif composition. The promoters of ZmLBD genes contain multiple types of cis-acting elements. In addition, by constructing the regulatory network of ZmLBD and other genes and miRNAs, 12 and 22 ZmLBDs were found to be involved in the gene regulatory network and miRNA regulatory network, respectively. The expression pattern analysis suggests that ZmLBD genes may be involved in different biological pathways, and drought stress induced the expressions of two inbred lines. The findings enhance our comprehension of the potential roles of the ZmLBD gene family in maize growth and development, which is pivotal for genetic enhancement and breeding efforts pertaining to this significant crop.

A Unifying Model of Mixed Inertial Modes in the Sun

We present an analytical model that unifies many of the inertial waves that have been recently observed on the surface of the Sun, as well as many other modes that have been theoretically predicted—but have yet to be observed—into a single family of mixed inertial modes. By mixed, we mean that the prograde- and retrograde-propagating members of this family have different restoring forces and hence different behavior. Thermal Rossby waves exist as prograde-propagating waves, while the high-frequency retrograde (HFR) wave is one example of a member of the retrograde branch. This family of mixed modes has fully 3D motions that satisfy the anelastic form of the continuity condition. As such, the horizontal velocity is both vortical and divergent with the later flow component associated with a dynamically important radial velocity. The modes are differentiated by the number of nodes in latitude, with the lowest latitudinal order corresponding to the traditional thermal Rossby wave of Busse, the first latitudinal overtone to the mixed mode of Bekki et al., and the second overtone to the HFR wave discovered by Hanson et al. There also exist infinitely more modes of higher latitudinal order whose frequencies increase as the order increases. These higher overtones may correspond to many of the inertial modes that have been recently identified by numerical eigenmode solvers.

Research on the Construction of the Organizational Mechanism of Rural Party Branches

Xi Jinping stressed that party members and cadres must be politically strong and strong, and the party organization must have superb leadership ability to provide a strong political guarantee for the implementation of the rural revitalization strategy. The problems in the organizational construction of rural party branches are mainly manifested in the imperfect selection mechanism of branch members, the loss of a large number of outstanding local talents, and the weak economic awareness of rural party members. Therefore, how to build a complete organizational mechanism of rural party branches around the three aspects is the core of improving the leadership ability of rural party branches, and it is also the key to giving full play to the leadership of rural party branches to lead the people to achieve rural revitalization.

Pengaruh Pelayanan, Nisbah Bagi Hasil, Dan Pengetahuan Koperasi Syariah Terhadap Keputusan Menabung Anggota KSPPS BMT Surya Abadi Riyanto Cabang Seputih Raman

This study aims to determine the effect of service, profit sharing ratio, and knowledge of Sharia Cooperatives on the decision to save members of KSPPS BMT Surya Abadi Riyanto Seputih Raman Branch. There is still a lack of people who decide to save or become members of KSPPS BMT Surya Abadi Riyanto, Seputih Raman branch, which is caused by several factors such as, from the perspective of members who save, there are still many complaints from members regarding poor service, inadequate facilities, and the views of members who are still minimal about Islamic cooperatives. In this study using analytical tools, namely SPSS (Static Package For Social Science). This study uses a type of Quantitative research, with a population of ± 3000 members, and a sample determined using the Slovin formula of 100 respondents. By doing several tests, namely, Validity Test, Reliability Test, Normality Test, Multicollinearity Test, Heteroscedasticity Test, Multiple Regression Analysis, t test, F test and Determinant Test (R2). Based on the results of the simultaneous test, it is obtained that the significant value is 0.000 <0.05 and the F-count value is 1231.804> 2.699, which means that there is a simultaneous influence between the Service variable (X1), Profit Sharing Rate (X2), and Sharia Cooperative Knowledge (X3) on the Saving Decision variable (Y). While the figure for R square is 0.975 or 97.5%. This shows that Service, Profit Sharing Rate, and Sharia Cooperative Knowledge have an effect of 97.5% on the Saving Decision of KSPPS BMT Surya Abadi Riyanto Branch Seputih Raman Members. The results showed that simultaneously Service, Profit Sharing Rate and Sharia Cooperative Knowledge had a significant effect on Member Saving Decisions, KSPPS BMT Surya Abadi Riyanto, Seputih Raman Branch should improve Services both in terms of facilities and infrastructure owned such as adding a queuing system, replacing employee support tools that have been worn out, provide a Profit Sharing Rate that is more profitable for members who save, and further educate or provide information so that members and prospective members (the public) have good Sharia Cooperative Knowledge such as products and services offered by KSPPS BMT Surya Abadi Riyanto, Seputih Raman Branch, so as to increase the Decision to Save Members and Prospective Members.

A platypterygiid ichthyosaur from the Cenomanian of central New Zealand

ABSTRACT Understanding of Cretaceous ichthyosaur diversity has substantially increased in recent years; however, Eastern Gondwana’s ichthyosaur fauna is still poorly understood, particularly in New Zealand where only scarce fragmentary material is known. A Cenomanian-aged partial ichthyosaur skeleton has been recovered from the northeastern South Island of New Zealand and is described here. Whilst the specimen is too fragmentary to formally name, this taxon shows an extreme reduction of the basioccipital extracondylar area, a scapula with a prominent acromion process and a strap-like scapula shaft, as well as a complete left pelvic girdle with an elongated depression on the anteroproximal face of the ischiopubis. We suggest that it is a late branching member of the platypterygiid ichthyosaurs, closely related to the Eastern Gondwanan ichthyosaur Platypterygius australis and to many European Cretaceous taxa. However, it appears to be unrelated to the Cretaceous ichthyosaurs of Western Gondwana, suggesting potential regionalism amongst the Gondwanan Cretaceous ichthyosaur populations.

The influence of biological relatedness on sexual dimorphism and sex classification based on external morphology of the frontal bone.

The most significant sexual differences in the human skull are located in the upper third of the face (the frontal bone), which is a useful research object, mainly in combination with virtual anthropology methods. However, the influence of biological relatedness on sexual dimorphism and frontal bone variability remains unknown. This study was directed at sexual difference description and sex classification using the form and shape of the external surface of the frontal bones from a genealogically documented Central European osteological sample (nineteenth to twentieth centuries). The study sample consisted of 47 cranial CT images of the adult members of several branches of one family group over 4 generations. Three-dimensional virtual models of the frontal bones were analyzed using geometric morphometrics and multidimensional statistics. Almost the entire external frontal surface was significantly different between males and females, especially in form. Significant differences were also found between this related sample and an unrelated one. Sex estimation of the biologically related individuals was performed using the classification models developed on a sample of unrelated individuals from the recent Czech population (Čechová et al. in Int J Legal Med 133: 1285 1294, 2019), with a result of 74.46% and 63.83% in form and shape, respectively. Failure of this classifier was caused by the existence of typical traits found in the biologically related sample different from the usual manifestation of sexual dimorphism. This can be explained as due to the increased degree of similarity and the reduction of variability in biologically related individuals. The results show the importance of testing previously published methods on genealogical data.

“Waiters’ dictatorship…”. The fight of food establishments in Warsaw and Lublin in 1919 in the local press

Poland’s regaining of independence in 1918 contributed to increased activi­ty of workers’ movements. Employees who wanted improved working con­ditions, normalised work hours and increased wages in many cases organi­sed demonstrations to achieve their goals. Members of the Trade Union of Waiters with the central office in Warsaw, who could not communicate with the stubborn owners of restaurants and cafes, undertook atypical forms of striking, which sometimes became a threat to the health and lives of the customers. Members of the Lublin branch, following the example of the wa­iters from Warsaw, organised many protests in restaurants, at the same time disputing with their employers in the local press.

PENDAMPINGAN CALON PESERTA ENGLISH SPEECH COMPETITION PORSENI PAC IPNU IPPNU KECAMATAN BUARAN

English is an international language which must be mastered by everyone including the members of IPNU and IPPNU. There were many competitions which were held to support English mastery, one of them is English speech competition. This competition motivated the members of IPNU and IPPNU to develop their English mastery. The purpose of this community service was to give accompaniment to the members of IPPNU Simbang Kulon branch so that they were able to write English speech script and they were able to perform it fluently and confidently in the IPNU IPPNU PORSENI Kecamatan Buaran in2023. The participants of this community service were the members of IPPNU Simbang Kulon branch. This community service was done in some steps. They were planning, implementation, evaluation, and report writing. The result of this community service showed that after joining this agenda, the participants could write English speech script based on the topic, using correct grammar and vocabulary. In addition, the participants were able to perform the speech confidently with the gesture, mimic, and eye contact. The participants were able to pronounce the utterance well and delivered the speech fluently. This community service was done successfully. It was proven that the one of the participants became the winner of the English speech competition in the PORSENI.

Multiple populations and a CH star found in the 300S globular cluster stellar stream

ABSTRACT Milky Way globular clusters (GCs) display chemical enrichment in a phenomenon called multiple stellar populations (MSPs). While the enrichment mechanism is not fully understood, there is a correlation between a cluster’s mass and the fraction of enriched stars found therein. However, present-day GC masses are often smaller than their masses at the time of formation due to dynamical mass-loss. In this work, we explore the relationship between mass and MSPs using the stellar stream 300S. We present the chemical abundances of eight red giant branch member stars in 300S with high-resolution spectroscopy from Magellan/MIKE. We identify one enriched star characteristic of MSPs and no detectable metallicity dispersion, confirming that the progenitor of 300S was a GC. The fraction of enriched stars (12.5 per cent) observed in our 300S stars is less than the 50 per cent of stars found enriched in Milky Way GCs of comparable present-day mass (∼104.5 $\mathrm{\, {\rm M}_{\odot }}$). We calculate the mass of 300S’s progenitor and compare it to the initial masses of intact GCs, finding that 300S aligns well with the trend between the system mass at formation and enrichment. 300S’s progenitor may straddle the critical mass threshold for the formation of MSPs and can therefore serve as a benchmark for the stellar enrichment process. Additionally, we identify a CH star, with high abundances of s-process elements, probably accreted from a binary companion. The rarity of such binaries in intact GCs may imply stellar streams permit the survival of binaries that would otherwise be disrupted.