MeHg In Sediments Research Articles

Algal-derived dissolved organic matter accelerates mercury methylation under cyanobacterial blooms in the sediment of eutrophic lakes

Mercury (Hg), especially in the form of methylmercury (MeHg), poses a significant threat to both organisms and the environment due to its extreme toxicity. While methylation process of Hg in sediments has been extensively studied, recognition of its associated risks and mechanisms during cyanobacterial blooms remains limited. This study investigated the distribution characteristics of Hg and MeHg in sediments of Taihu Lake, China. The concentration of Hg and MeHg varied within the range of 96.0–212.0 ng g−1 and 0.1–0.5 ng g−1, respectively. Higher ecological risks of Hg were found in algal-dominated regions compared to macrophyte areas. The significant correlations observed between Hg, MeHg, and algal-derived dissolved organic matter (ADOM) components C1 and C2 in algal-dominated regions indicate a close association between ADOM components and the Hg methylation process. These components are involved in the absorption or complexation of Hg, participate in redox reactions, and modulate microbial activity. The dsrB gene in sulfate-reducing bacteria (SRB) was found to accelerate the metabolic pathways of Hg methylation. These findings indicate that ADOM could enhance the methylation process of Hg during cyanobacterial blooms, which warrants attention.

Non-mercury methylating microbial taxa are integral to understanding links between mercury methylation and elemental cycles in marine and freshwater sediments

The goal of this study was to explore the role of non-mercury (Hg) methylating taxa in mercury methylation and to identify potential links between elemental cycles and Hg methylation. Statistical approaches were utilized to investigate the microbial community and biochemical functions in relation to methylmercury (MeHg) concentrations in marine and freshwater sediments. Sediments were collected from the methylation zone (top 15 cm) in four Hg-contaminated sites. Both abiotic (e.g., sulfate, sulfide, iron, salinity, total organic matter, etc.) and biotic factors (e.g., hgcA, abundances of methylating and non-methylating taxa) were quantified. Random forest and stepwise regression were performed to assess whether non-methylating taxa were significantly associated with MeHg concentration. Co-occurrence and functional network analyses were constructed to explore associations between taxa by examining microbial community structure, composition, and biochemical functions across sites. Regression analysis showed that approximately 80% of the variability in sediment MeHg concentration was predicted by total mercury concentration, the abundances of Hg methylating taxa, and the abundances of the non-Hg methylating taxa. The co-occurrence networks identified Paludibacteraceae and Syntrophorhabdaceae as keystone non Hg methylating taxa in multiple sites, indicating the potential for syntrophic interactions with Hg methylators. Strong associations were also observed between methanogens and sulfate-reducing bacteria, which were likely symbiotic associations. The functional network results suggested that non-Hg methylating taxa play important roles in sulfur respiration, nitrogen respiration, and the carbon metabolism-related functions methylotrophy, methanotrophy, and chemoheterotrophy. Interestingly, keystone functions varied by site and did not involve carbon- and sulfur-related functions only. Our findings highlight associations between methylating and non-methylating taxa and sulfur, carbon, and nitrogen cycles in sediment methylation zones, with implications for predicting and understanding the impact of climate and land/sea use changes on Hg methylation.

Anthropogenic activities enhance mercury methylation in sediments of a multifunctional lake: Evidence from dissolved organic matter and mercury-methylating microorganisms

Multifunctional lakes are highly susceptible to anthropogenic influences, potentially introducing exogenous pollutants or nutrients into aquatic sediments. This, in turn, affects the mercury (Hg) methylation in the sediments. This study was conducted in the Changshou Lake, a representative multifunctional lake in southwestern China, with a specific focus on investigating the Hg variations, the potential of Hg methylation, and the influential factors affecting the methylation process within sediments across different functional areas. The results revealed significant variations in total Hg concentrations between the ecological culture area (area I), the ecological tourism area (area II), and the wetland protection area (area III), suggesting the possibility of exogenous Hg introduction associated with human activities. Furthermore, sediments from areas I and II displayed a greater potential for Hg methylation. This was ascribed to the enhanced diversity and relative abundance of Hg-methylating microorganisms, especially Geobacteraceae, induced by elevated levels of dissolved organic carbon in these two areas from human activities like historical cage culture. This study provides evidence that anthropogenic activities enhance the process of Hg methylation in the sediments of multifunctional lakes, highlighting the necessity of implementing comprehensive scientific water quality management practices to mitigate the negative impacts of human influences on these unique ecosystems.

Cross-shelf processes of terrigenous organic matter drive mercury speciation on the east siberian shelf in the Arctic Ocean

The cross-shelf distributions of total mercury (THg), methylmercury (MeHg) and organic and inorganic matter, as well as the presence of the hgcA gene were investigated on the East Siberian Shelf (ESS) to understand the processes underlying the speciation of sedimentary Hg. Samples were collected from 12 stations grouped into four zones based on water depth: inner shelf (5 stations), mid-shelf (3 stations), outer shelf (2 stations), and slope (2 stations). The THg concentration in the surface sediment increased from the inner shelf (0.25 ± 0.023 nmol g−1) toward the slope (0.52 nmol g−1), and, when normalized to total organic carbon content, the THg showed a positive correlation with the clay-to-sand ratio (r2 = 0.48, p = 0.012) and degree of chemical weathering (r2 = 0.79, p = 0.0001). The highest MeHg concentrations (3.0 ± 1.8 pmol g−1), as well as peaks in the S/C ratio (0.012 ± 0.002) of sediment-leached organic matter, were found on the mid-shelf, suggesting that the activities of sulfate reducers control the net Hg(II) methylation rates in the sediment. This was supported by results from a principal component analysis (PCA) performed with Hg species concentrations and sediment-leached organic matter compositions. The site-specific variation in MeHg showed the highest similarity with that of CHONS compounds in the PCA, where Deltaproteobacteria were projected to be putative Hg(II) methylators in the gene analysis. In summary, the hydrodynamic sorting of lithogenic particles appears to govern the cross-shelf distribution of THg, and in situ methylation is considered a major source of MeHg in the ESS sediment.

Mercury concentrations in sediments and oysters in a temperate coastal zone: a comparison of farmed and wild varieties.

Oyster aquaculture has progressively increased to meet growing demands for seafood worldwide; however, its effects on methylmercury (MeHg) production in sediment and accumulation in oysters are largely unknown. In this study, total Hg (THg) and MeHg in sediments collected from aquaculture and reference sites and in farmed and wild oysters were measured and compared to explore potential factors that regulate MeHg production and bioaccumulation at the aquaculture sites. The results showed that the mean concentrations of THg and MeHg in varying sediment depths at the aquaculture site were 34 ± 4.1 ng g-1 and 16 ± 12 pg g-1, respectively. In comparison, the mean concentrations of THg and MeHg in sediments at the reference site were 25 ± 2.5 ng g-1 and 63 ± 28 pg g-1, respectively. While the MeHg/THg in the aquaculture sediments increased with organic carbon content, the slope of MeHg/THg versus organic carbon content was suppressed by high concentrations of dissolved sulfide in the pore water. Multiple parameters (total sulfur, total nitrogen and acid volatile sulfide in sediment, and dissolved sulfide in pore water) showed significant negative relationships with MeHg/THg in the sediment, and the total sulfur content in the sediment showed the highest inverse correlation factor with MeHg/THg (r = - 0.83). The mean concentrations of THg and MeHg in farmed oysters (mean weight 3.2 ± 1.5 g) were 36 ± 10 ng g-1 and 15 ± 6.7 ng g-1, respectively, while those in wild oysters (mean weight 0.92 ± 0.32 g) were 47 ± 9.9 ng g-1 and 15 ± 6.7 ng g-1, respectively. Concerning oysters of the same size range, THg and MeHg levels were higher in farmed oysters than in wild oysters despite the faster growth rate of farmed oysters, suggesting that the Hg content of food sources is more important than growth dilution rates in the control of Hg levels. The mean hazardous quotient for MeHg in farmed oyster was calculated as 0.044 ± 0.020, suggesting no expected health risk from farmed oyster consumption.

Mercury biomagnification and trophic structure patterns in neotropical coastal estuaries impacted by a Chlor-alkali plant in northeast Brazil

The present study reports the concentration of HgT and MeHg in sediments and food webs in estuarine and coastal areas of Northeast Brazil, which have been subject to different impacts, mainly by chlorine and alkali companies. The results showed that the HgT in the sediment varied from 1.7 to 1186 ng g−1 and from 3.3 to 2163 ng g−1 in the organisms. A strong positive relationship between the levels of Hg in the sediment and the organisms was detected. Methylmercury represented 42 to 99% in the muscles of fish and invertebrates. Biomagnification processes were identified for all study areas evaluated according the trophic magnification factor (TMF), which ranged from 3.1 to 12.3. We found significant negative correlations with δ13C values and mercury concentrations and positive correlations between δ15N values and mercury, typical of Hg trophodynamics. This suggests a direct impact of the level of site-specific contamination on local food web and the ecosystem, making continuous monitoring studies necessary to assess food security and the risks that the riverine community is exposed as a result of ingesting mercury contaminated fish.

Geochemical controls on the distribution of total mercury and methylmercury in sediments and porewater from the Yangtze River Estuary to the East China Sea

A comprehensive investigation was conducted to explore the distributions of total mercury (THg) and methylmercury (MeHg) in sediments and porewater along a typical transect from the Yangtze River Estuary (YRE) to the East China Sea (ECS) open shelf. THg concentrations in the surface sediments exhibited large variations across sites, higher in the estuary mixing region, especially within the turbidity maximum zone (TMZ). The spatial and vertical distributions (0–20 cm) of THg in sediments were highly controlled by sediment grain size and total organic carbon (TOC), due to the strong binding between Hg and fine-grained sediments that enriched with organic matter. In contrast, MeHg concentrations in surface sediments were higher in the estuary mixing region and the ECS open shelf than in the river channel, with remarkably higher MeHg/THg ratios in sediments and porewater at the open shelf sites, identified as the regional hotspots of the net in situ MeHg production. Considering the large gradients of physiochemical properties of sediments, porewater and the overlying water, the results of this study suggested that the higher net Hg methylation potential in the open shelf region was largely attributed to the lower acid volatile sulfide, lower TOC and higher salinity, which facilitated the partitioning of inorganic Hg into porewater that highly bioavailable for Hg-methylation bacteria. Moreover, the estimated diffusive fluxes of MeHg at the sediment-water interface were positive at all the tested sites, and pronouncedly higher within the TMZ (driven by the higher THg loading and higher porosity) that requires special attention.

Spatial and seasonal patterns of mercury concentrations, methylation and demethylation in central Canadian boreal soils and stream sediment

Terrestrial ecosystems store large amounts of mercury (Hg), which may be subject to methylation, mobilization and uptake into downstream aquatic ecosystems. Mercury concentrations, methylation and demethylation potentials are not well characterized simultaneously across different habitats in boreal forest ecosystems, particularly not so in stream sediment, leading to uncertainties about the importance of various habitats as primary production areas of the bioaccumulative neurotoxin methylmercury (MeHg). In this study, we collected soil and sediment samples from 17 undisturbed, central Canadian boreal forested watersheds during spring, summer and fall to robustly characterize the spatial (upland and riparian/wetland soils, and stream sediment) and seasonal patterns of total Hg (THg) and MeHg concentrations. Mercury methylation and MeHg demethylation potentials (Kmeth and Kdemeth) in the soils and sediment were also assessed using enriched stable Hg isotope assays. We found the highest Kmeth and %-MeHg in stream sediment. In both riparian and wetland soils, Hg methylation was lower and less seasonally variable compared to stream sediment, but had comparable MeHg concentrations, suggesting longer-term storage of MeHg produced in these soils. Soil and sediment carbon content, and THg and MeHg concentrations were strong covariates across habitats. Additionally, sediment carbon content was important for delineating between stream sediment with relatively high vs. relatively low Hg methylation potential, which generally separated between different landscape physiographies. Broadly, this large and spatiotemporally diverse dataset is an important baseline for understanding Hg biogeochemistry in boreal forests both in Canada and possibly in many other boreal systems globally. This work is particularly important with respect to future possible impacts from natural and anthropogenic perturbations, which are increasingly straining boreal ecosystems in various parts of the world.

An examination of the factors influencing the bioaccumulation of methylmercury at the base of the estuarine food web

Estuarine systems have received ongoing mercury (Hg) inputs from both point sources and regional contamination and have high legacy Hg in sediments. This is an environmental concern given that coastal seafood is an important vector for human exposure to methylmercury (MeHg). The base of the food chain represents the most important trophic steps for MeHg bioaccumulation. The magnitude of the uptake by phytoplankton, and their consumers, is influenced by many factors, in addition to sediment and water MeHg concentrations, that impact MeHg assimilation into phytoplankton and the trophic transfer to higher trophic levels, both benthic and pelagic. For forage fish, such as mummichogs (Fundulus heteroclitus), abiotic and biotic (bioenergetic) factors can influence their MeHg content, and diet is also important as they feed both on benthic and pelagic prey. Given that the importance of sediment MeHg versus pelagic MeHg sources has been debated, we updated a phytoplankton bioaccumulation model, and coupled this with a bioaccumulation model for MeHg concentration in mummichog tissue to examine the controlling factors for sites, from Maine to Maryland, USA, ranging widely in their Hg concentrations and other variables. The study highlighted the importance of DOC in modulating uptake into the pelagic food web, but also demonstrated the importance of diet in controlling mummichog MeHg. Finally, the relative importance of MeHg source - sediment or water column – was correlated with the level of Hg contamination. Sediment-derived MeHg was a more important source for highly Hg contaminated systems. As water column and sediment MeHg are not strongly correlated for the studied ecosystems, their importance as a source of MeHg to mummichogs varies with location. The study highlights the differences across ecosystems in MeHg bioaccumulation pathways, and that uptake into phytoplankton is an important variable controlling forage fish concentration.

Impacts of River Damming on Sediment Methylmercury Dynamics

Reservoirs are reported to stimulate mercury (Hg) methylation and thereby affect methylmercury (MeHg) dynamics in rivers by trapping sediment and increasing the hydraulic residence time (HRT). However, whether there are similarities in sediment %MeHg (MeHg/Hg) in relation to reservoir properties for different rivers and their associated mechanisms remain unknown. Here, we investigated the sediment MeHg in two heavily dammed rivers and one naturally flowing river in the same region. We found that river damming results in the accumulation of sediment Hg and OC and a higher abundance of hgcAB, thus elevating the sediment %MeHg. Sediment %MeHg showed a positive correlation with reservoir HRT, and the methylation time of Hg acts as the threshold value to determine the impact of river damming on sediment %MeHg. However, sediment %MeHg had either a positive or a negative correlation with reservoir age in different rivers, and the effect of reservoir age on sediment %MeHg depends on whether the stock of substances in the reservoir has a dominance of internal consumption or is one of external supply. These findings provide an important reference for further research on Hg biogeochemical cycling and MeHg risk management in dammed rivers.

Occurrence and risk assessment of total mercury, methylmercury and other selected trace metals in the surface sediments of the Cochin Estuary, Southwest Coast of India

This study assesses the ecological risk and impact of anthropogenic activities in the Cochin estuary – a Ramsar site – by trace metal indices in surface sediment collected from thirteen sampling stations in three seasons. The trace metals analyzed are THg, MeHg, Pb, Cu, Cd, Zn, Ni, Co, Mn, Cr, Fe, and Al in the surface sediments and their spatio-temporal variation is recorded. Pollution indices (i.e., Enrichment factor, Index of geoaccumulation, sediment quality guidelines, pollution load index, and potential ecological risk index) were applied to ascertain the sediment quality. The distribution pattern of Cr, Zn, and Cd revealed higher concentrations in the northern arm of the estuary and near the industrial regions indicating non-geogenic inputs. The concentration of THg and MeHg exhibited spatial and temporal variations and were higher than the background values. The results showed that higher levels of trace metals were found in the northern part of the Cochin Estuary, which could be attributed to the weak flow and significant input of industrial effluents.

Toward efficient bioremediation of methylmercury in sediment using merB overexpressed Escherichia coli

Sediment is the primary hotspot for microbial production of toxic and bio-accumulative methylmercury (MeHg). Common remediation strategies such as sediment dredging and capping can be too expensive and cannot degrade MeHg efficiently. Here, we constructed an Escherichia coli strain overexpressing merB gene (DH5α J23106) and assessed the effectiveness of this recombinant strain in degradation of MeHg in culture medium and sediment. DH5α J23106 can efficiently degrade MeHg (with initial concentration from 0.01 to 50 ng/mL) to more than 81.6% in a culture medium under anoxic and oxic conditions. Enriched isotope addition (199HgCl2) revealed that this recombinant strain can degrade 78.6% of newly produced Me199Hg in actual sediment, however the biodegradation decreased to 36.3% for intrinsic MeHg. Degradation of spiked MeHg after aging in anoxic and oxic sediments further demonstrated DH5α J23106 can efficiently degrade newly produced MeHg and the degradation decreased with aging significantly, especially for oxic sediment. Eight sediments were further assessed for the biodegradation of aged MeHg by DH5α J23106 under oxic conditions, with degradation ratios ranging from 9.0% to 66.9%. When combined with (NH4)2S2O3 leaching, the degradation of MeHg increased by 15.8–38.8% in on-site and off-site modes through enhanced MeHg bioavailability in some of these sediments. Thus, this recombinant strain DH5α J23106 can degrade MeHg efficiently and have the potential for remediating bioavailable MeHg in contaminated sediments.

Evaluating the influence of seasonal stratification on mercury methylation rates in the water column and sediment in a contaminated section of a western U.S.A. reservoir

Mercury methylation frequently occurs at the active oxic/anoxic boundary between the sediment bed and water column of lakes and reservoirs. Previous studies suggest that the predominant mercury methylation zone moves to the water column during periods of stratification and that high potential methylation rates (Km) in sediment require oxygenated overlying water. However, simultaneous measurements of methylmercury (MeHg) production in both the sediment and water column remain limited. Understanding the relative importance of sediment versus water column methylation and the impact of seasonal stratification on these processes has important implications for managing MeHg production. This study measured Km and potential demethylation rates (Kdm) using stable isotope tracers of unfiltered inorganic mercury and MeHg in sediments and water of the littoral and profundal zones of a shallow branch of the Nacimiento Reservoir in California's central coastal range. Field sampling was conducted once during winter (well-mixed/oxygenated conditions) and once during late summer (thermally stratified/anoxic conditions). The results showed very high ambient MeHg concentrations in hypolimnetic waters (up to 7.5 ng L−1; 79% MeHg/total Hg). During late summer, littoral sediments had higher Km (0.024 day−1) compared to profundal sediments (0.013 day−1). Anoxic water column Km were of similar magnitude to Km in the sediment (0.03 day−1). Following turnover, profundal sediment Km did not change significantly, but water column Km became insignificant. Summer and winter sediment Kdm were higher in profundal (2.35, 3.54 day−1, respectively) compared to the littoral sediments (0.52, 2.56 day−1, respectively). When modelled, Km in the water column could account for approximately 40% of the hypolimnetic MeHg. Our modelling results show that the remaining MeHg in the hypolimnion could originate from the profundal sediment. While further study is needed, these results suggest that addressing methylation in the water column and profundal sediment are of equal importance to any remediation strategy.

Declines of methylmercury along a salinity gradient in a low-lying coastal wetland ecosystem at South Carolina, USA

Wetlands are widely regarded as biogeochemical hotspots of highly toxic methylmercury (MeHg), mainly mediated by sulfate-reducing bacteria. In low-lying coastal wetlands, sea level rise, a phenomenon caused by global climate change, is slowly degrading numerous healthy freshwater forested wetlands into salt-degraded counterparts with a nickname “ghost forests”, and eventually converting them to saltmarshes. However, little is known about the changes of mercury (Hg) methylation, bioaccumulation, and biomagnification along the forest-to-saltmarsh gradient. Here, we conducted extensive field sampling in three wetland states (healthy forested wetlands, salt-degraded forested wetlands, and saltmarsh) along a salinity gradient (from 0 to 9.4 ppt) in Winyah Bay, South Carolina, USA. We found that in our study wetland systems the saltmarshes had the lowest levels of both total Hg and MeHg in sediments and biota, as compared to healthy forested wetlands and saltwater-degraded ghost forests. Our results suggest that the slow conversion of forested wetland to saltmarsh could reduce net MeHg production in our study wetland systems, which we hypothesized that could be attributed to increased sulfate reduction and excessive buildup of sulfide in sediment that inhibits microbial Hg methylation, and/or reduced canopy density and increased photodegradation of MeHg. However, it should be noted that biogeochemical MeHg responses to salinity changes may be site-specific and we urge more similar studies in other wetland systems along a salinity gradient. Therefore, long-term salinization of coastal wetlands and the slow conversion of forests to marshes could decrease long-term exposure of toxic MeHg levels in coastal food webs that are similar to our system, and ultimately reduce human exposure to this neurotoxin.

Effects of Herbaceous Plants on Methylmercury Net Production and Release From Sediment After Flooding in the Water-Level Fluctuation Zone of the Jialing River.

To explore the effects of herbaceous plants on mercury (Hg) behaviors after flooding in the water-level fluctuation zone (WLFZ) of Jialing River, three typical local plants (D. pyramidalis, A. philoxeroides and C. dactylon) and their in-situ sediments were collected for flooding experiments to study the Hg dynamics of water and sediment in different treatments. The results showed that flooding increased sediment MeHg concentrations and flooded plants (especially for the D. pyramidalis) promoted this process. Similarly, the highest dissolved MeHg level and proportion of MeHg to total Hg (%MeHg) were observed in plant-water treatments in the presence of D. pyramidalis, indicating the potential for the methylation of Hg in the water body influenced by the decay process of herbaceous plants. These results suggest that the herbaceous plant D. pyramidalis contributes more to Hg methylation and release in the WLFZ of the Jialing River than other plants studied.

Impacts of Forest Fire Ash on Aquatic Mercury Cycling.

Mercury (Hg) is a ubiquitous contaminant in the environment and its methylated form, methylmercury (MeHg), poses a worldwide health concern for humans and wildlife, primarily through fish consumption. Global production of forest fire ash, derived from wildfires and prescribed burns, is rapidly increasing due to a warming climate, but their interactions with aqueous and sedimentary Hg are poorly understood. Herein, we compared the differences of wildfire ash with activated carbon and biochar on the sorption of aqueous inorganic Hg and sedimentary Hg methylation. Sorption of aqueous inorganic Hg was greatest for wildfire ash materials (up to 0.21 μg g-1 or 2.2 μg g-1 C) among all of the solid sorbents evaluated. A similar Hg adsorption mechanism for activated carbon, biochar made of walnut, and wildfire ash was found that involves the formation of complexes between Hg and oxygen-containing functional groups, especially the -COO group. Notably, increasing dissolved organic matter from 2.4 to 70 mg C L-1 remarkably reduced Hg sorption (up to 40% reduction) and increased the time required to reach Hg-sorbent pseudo-equilibrium. Surprisingly, biochar and wildfire ash, but not activated carbon, stimulated MeHg production during anoxic sediment incubation, possibly due to the release of labile organic matter. Overall, our study indicates that while wildfire ash can sequester aqueous Hg, the leaching of its labile organic matter may promote production of toxic MeHg in anoxic sediments, which has an important implication for potential MeHg contamination in downstream aquatic ecosystems after wildfires.

Impact of sulfur-impregnated biochar amendment on microbial communities and mercury methylation in contaminated sediment

S-impregnation of biochar through elemental S streaming is known to increase its sorption performance against Hg and methyl mercury (MeHg). However, the effects of %S-loading on biochar’s mechanism and sorption capacities for MeHg, and its consequent impact when used as an amendment material for Hg-contaminated sediments, are poorly understood, and thus, were investigated in this work. Our results showed that a minimum sulfur loading of 1% was the most effective in reducing MeHg levels in sediments. At higher %S-loading (3–20%), the reduction in surface area, pore blockage due to unreacted sulfur particles, and presence of poorly bound sulfur species resulted in lowered effectiveness for MeHg control. Increasing S-functionalization during impregnation shifted the sorption process of MeHg from Hg-O to Hg-S in S-impregnated biochar (BCS). Our 60-day slurry experiment showed a significant reduction in pore water THg (40–70%) and MeHg (30–55%), as well as sediment MeHg (50–60%) in biochar-amended sediments. The reduction in the bioavailable Hg resulted in lowered Hg methylation, as supported by the suppression of both the Fe- and SO42--reduction activities in the amended sediments. The microbial community structure in BCS-amended sediments showed a shift towards sulfur-consuming, iron-reducing, thiosulfate-oxidizing, and sulfate-reducing bacterial populations. At the genus level, the overall relative abundance of principal Hg methylators was also lower in the BCS treatment than in the unamended sediments. This study highlights the application of BCS as a promising strategy for remediation of Hg-contaminated sediments.

Grass Shrimp (Palaemonetes pugio) as a Trophic Link for Methylmercury Accumulation in Urban Salt Marshes.

Grass shrimp (Palaemonetes pugio) represent a potential link in the transfer of methylmercury (MeHg) from salt marsh sediments to transient young-of-the-year (YOY) fish. Across six salt marshes subject to varying degrees of Hg contamination, MeHg concentration in grass shrimp was significantly correlated with MeHg in sediment (p < 0.05, R2 = 0.81). Bioenergetic models show that grass shrimp alone account for 12-90% of MeHg observed in YOY striped bass and 6-22% of MeHg in YOY summer flounder. Direct accumulation of MeHg from grass shrimp to YOY fish increased with MeHg levels in grass shrimp and sediment. However, in the most contaminated salt marshes with the highest levels of MeHg in grass shrimp and sediment, indirect accumulation of MeHg from grass shrimp by YOY summer flounder, whose diet is dominated by benthic forage fish (mummichog), is predicted to plateau because higher concentrations of MeHg in grass shrimp are offset by a lower proportion of grass shrimp in the mummichog diet. Our results demonstrate that grass shrimp are an important trophic link in the bioaccumulation of MeHg in salt marsh food webs and that MeHg accumulation in YOY fish varies with both the concentration of MeHg in salt marsh sediments and benthic food web structure.

Seasonal trends of mercury bioaccumulation and assessment of toxic effects in Asian clams and microbial community from field study of estuarine sediment

This study investigated seasonal trends in bioaccumulation potential and toxic effects of mercury (Hg) in Asian clams (Corbicula fluminea) and microbial community. For this, a clam-exposure experiment was performed during summer, fall, and winter seasons in four different sites (HS1: control/clean site; HS2, HS3, and HS4: contaminated sites) of Hyeongsan River estuary, South Korea. Total mercury (THg) and methylmercury (MeHg) in whole sediments were highest at HS4 site during fall, sustained similar levels during winter, but decreased during summer. Unlike whole sediment, pore water reported higher levels in summer, and gradually declined during fall and winter. Asian clams from HS4 site collected during summer presented highest bioaccumulations of THg (521.52 μg/kg, dry weight) and MeHg (161.04 μg/kg, dry weight), which also correlated with the higher levels of Hg present in pore water in the same season. Moreover, biota-sediment-pore water accumulation factor (BSpAF) were comparatively greater in clams collected from HS2∼HS4 compared to HS1 sites, suggesting that porewater was a better indicator of accumulation of Hg. Upregulation of biomarker genes responsible for detoxifying process (gsts1), scavenging oxidative stress (cat), and protein reparation (hsp70 and hsp90) were observed in clams collected from HS2∼HS4. The overexpression of these biomarkers implied that Asian clams can be considered as promising warning tools for Hg-contamination. Both bacterial and metabolic diversities were negatively affected by higher levels of THg and MeHg. Phylum Proteobacteria was enriched in HS2∼HS4 compared to HS1. In contrast, phylum Bacteroidetes showed a reverse trend. The metabolic profile was highest in HS1 and lowest in HS4, revealing higher stress of Hg in HS4 site. Overall, the outcomes of this field study broaden the information on seasonal trends of bioaccumulation of Hg and its toxic effects. These findings may be helpful in Hg monitoring and management programs in other river systems.

An examination of mercury levels in the coastal environment and fish of Cote d’Ivoire

Artisanal and small-scale gold mining (ASGM), energy production and other industrial inputs are a major source of anthropogenic mercury (Hg) to the aquatic environment globally, and these inputs have led to environmental contamination and human exposure. While studies have documented the effects of Hg inputs to rivers and marine waters of the West African region, estuarine waters of Cote d’Ivoire have been understudied, besides the waters surrounding Abidjan. To fill this gap, and to examine the potential for human exposure to methylmercury (MeHg), we measured the concentrations of total Hg, MeHg, and ancillary parameters in water (dissolved and particulate phases), sediment and fish to determine the extent of environmental impact and the potential for MeHg exposure for people consuming these fish. Levels of Hg and MeHg in sediment were elevated in the vicinity of the urban environment (up to 0.3 ng/g dry weight (dw) MeHg and 623 ng/g dw total Hg) and lowest in the more remote estuarine environments. Measurements of Hg in tuna and other larger pelagic coastal species indicated that levels were elevated but comparable to other North Atlantic regions. However, levels of Hg in fish, even smaller estuarine species, were such that the rural and urban populations are potentially being exposed to unsafe levels of MeHg, primarily as a result of the relatively high fish consumption in Cote d’Ivoire compared to other countries. Overall, both local point sources and the transport of Hg used in interior ASGM activities are the sources for Hg contamination to these coastal waters.