Right ventricular (RV) failure is the major cause of mortality and morbidity in heart transplant patients. RV functional assessment of donor hearts using current technology such as echocardiogram cannot easily be conducted due to technical and anatomical issues. We used stereo Digital Image Correlation (DIC) (Fig. 1a) to characterize the RV motion and deformation in open-heart surgery (Fig.1b) and ex situ heart perfusion (ESHP) (Fig.1c). This work is a pilot study aiming to establish the framework for long term research. The system setup of stereo DIC shown in Fig. 1a used two 1.7 megapixel cameras (Basler acA1440-73gm) equipped with 16mm lenses. The equipment is installed on a custom-made frame with a stand on the floor. A piece of SURGICEL® pre-patterned with random dots using non-toxic sterile medical marker was applied on the interest surface of RV. Measurements with a frequency of 30Hz were performed on an open-chest in vivo pig heart (pig weight: 40kg) and ex situ perfused human heart (heart weight: 605g). All images were processed in VIC-3D 8 software. Fig. 1 shows two sets (pig heart and human heart) of results obtained from the DIC measurement. Results in each set were obtained from the same imaging sequence over consecutive heartbeats. In both human heart and pig heart measurement, each parameter has the same number of pulse and the shape of the pulses for each parameter have a high degree of similarity. RV motion and deformation values at different points show different pulse amplitudes. A noninvasive methodology was described for obtaining the RV functional data (e.g. principle strain, tissue velocity, displacement and etc.) using stereo DIC technology. The obtained measurements provide new insight into the biomechanics of the RV. This new approach is a promising tool for future RV functional assessment during surgery or ESHP.