Medium-sized Animals Research Articles

Pickup and Delivery Vehicle Routing Problem with Hybrid Missions and Third-Party Vehicle Rentals: A Case Study of Animal Feed Industry

The vehicle routing problem for small and medium-sized animal feed manufacturers is a complex challenge because each transport route can vary significantly between delivering products to customers and picking up raw materials to return to the factory. Currently, businesses lack well-planned management for utilizing a limited number of trucks. As a result, they often incur additional costs by relying on third-party vehicle rentals. This research aims to reduce transportation costs for small and medium-sized animal feed manufacturers by solving a formulated mixed-integer linear programming (MILP) model, using experimental data that accurately reflects the actual problems faced. The MILP model results help reduce transportation costs by up to 18.5% compared to current practices by optimizing route sequences and incorporating the use of third-party vehicle rentals. This also serves as a guideline for reducing transportation costs in other similar industries.

A Soft-Fiber Bioelectronic Device with Axon-Like Architecture Enables Reliable Neural Recording In Vivo under Vigorous Activities.

Implantable neural devices that record neurons in various states, including static states, light activities such as walking, and vigorous activities such as running, offer opportunities for understanding brain functions and dysfunctions. However, recording neurons under vigorous activities remains a long-standing challenge because it leads to intense brain deformation. Thus, three key requirements are needed simultaneously for neural devices, that is, low modulus, low specific interfacial impedance, and high electrical conductivity, to realize stable device/brain interfaces and high-quality transmission of neural signals. However, they always contradict each other in current material strategies. Here, a soft fiber neural device capable of stably tracking individual neurons in the deep brain of medium-sized animals under vigorous activity is reported. Inspired by the axon architecture, this fiber neural device is constructed with a conductive gel fiber possessing a network-in-liquid structure using conjugated polymers and liquid matrices and then insulated with soft fluorine rubber. This strategy reconciles the contradictions and simultaneously confers the fiber neural device with low modulus (300kPa), low specific impedance (579kΩµm2), and high electrical conductivity (32700Sm-1) - ≈1-3 times higher than hydrogels. Stable single-unit spike tracking in running cats, which promises new opportunities for neuroscience is demonstrated.

Movement and effectiveness of shelters for restocking of the sea cucumber Isostichopus badionotus

The sea cucumber Isostichopus badionotus has been overfished along its Mexican distribution, but no abundance recovery has been observed despite management efforts. Restocking and sea ranching strategies can use releasing individuals, and magnitude of protected areas be related on species dispersion knowledge. We measured the short-term movement paths on I. badionotus and their relation with environmental variables. In addition, the influence of biofilm presence/absence on artificial shelters surfaces and the use for animals were compared. Data was obtained through time-lapse cameras in experiments performed in outer aquaria under the natural fluctuation of temperature and sunlight. We observed that larger (Le) individuals exhibited greater daily movement distances (D) (D=0.89*Le −2.533 cm); furthermore, the sea cucumbers move faster in bare aquaria than aquaria with sand. The mean daily movement without sand was 518 cm for small sizes, 615 cm for medium, and 688 cm for large sizes. In the presence of sand, was 127, 196, and 315 cm, respectively. Movement was minimal in the morning/early afternoon (07:00–15:00), a maximum in the afternoon/evening (15:00–23:00), and an intermediate at night/early morning (23:00–07:00). The movement of the small sea cucumbers (>5.96−10.75 cm), was linked to light. Medium-sized and large sea cucumbers (>10.6−19.69 cm) respond to changes in temperature. We observed a higher rate of shelter use by small sea cucumbers (53 % of the day) and a greater affinity for pre-conditioned shelters (80 % of shelter use). Medium-sized and large animals did not show any shelter preferences. Results indicate low dispersion of I. badionotus on daily timescale. The affinity found in small animals towards shelters with biofilm, and their use only in this size class, makes them essential for survival in restoration strategies.

Validation of a low-cost continuous renal replacement therapy dialysate fluid controller for experimental purposes

BackgroundContinuous renal replacement therapy (CRRT) support is crucial for critically ill patients and it is underexplored in specific situations. Experimental CRRT offers a means to gain insights into these scenarios, but the prohibitive cost of CRRT machines limits their accessibility. This study aimed to develop and validate a low-cost and precise dialysate controller for experimental CRRT.ResultsOur results demonstrate a commendable level of precision in affluent flow control, with a robust correlation (R2 = 0.99) for continuous flow and a strong correlation (R2 = 0.95) for intermittent flow. Additionally, we observed acceptable agreement with a bias = 3.4 mL (upper limit 95% = 43.9 mL and lower limit 95% = − 37 mL) for continuous flow and bias = − 20.9 mL (upper limit 95% = 54 mL and lower limit 95% = − 95.7 mL) for intermittent flow, in this way, offering a precise CRRT dose for the subjects. Furthermore, we achieved excellent precision in the cumulative ultrafiltration net (UFnet), with a bias = − 2.8 mL (upper limit 95% = 6.5 mL and lower limit 95% = − 12 mL). These results remained consistent even at low affluent flow rates of 8, 12, and 20 mL/min, which are compatible with CRRT doses of 25–30 mL/kg for medium-sized animals. Moreover, the acceptable precision of our findings persisted when the dialysate controller was subjected to high filter dialysate chamber pressure for an extended duration, up to 797 min.ConclusionsThe low-cost dialysate controller developed and tested in this study offers a precise means of regulating CRRT in experimental settings. Its affordability and accuracy render it a valuable instrument for studying CRRT support in unconventional clinical scenarios, particularly in middle-income countries’ experimental ICU laboratories.

Animal Model for Anterior Lumbar Interbody Fusion: A Literature Review.

Lumbar interbody fusion (LIF) is a surgical procedure for treating lumbar spinal stenosis and deformities. It removes a spinal disc and insert a cage or bone graft to promote solid fusion. Extensive research on LIF has been supported by numerous animal studies, which are being developed to enhance fusion rates and reduce the complications associated with the procedure. In particular, the anterior approach is significant in LIF research and regenerative medicine studies concerning intervertebral discs, as it utilizes the disc and the entire vertebral body. Several animal models have been used for anterior LIF (ALIF), each with distinct characteristics. However, a comprehensive review of ALIF models in different animals is currently lacking. Medium-sized and large animals, such as dogs and sheep, have been employed as ALIF models because of their suitable spine size for surgery. Conversely, small animals, such as rats, are rarely employed as ALIF models because of anatomical challenges. However, recent advancements in surgical implants and techniques have gradually allowed rats in ALIF models. Ambitious studies utilizing small animal ALIF models will soon be conducted. This review aims to review the advantages and disadvantages of various animal models, commonly used approaches, and bone fusion rate, to provide valuable insights to researchers studying the spine.

LabPET II scanner performances improvement: Thermal stability control based on FPGA

The LabPET II detection module serves as the fundamental component within Positron Emission Tomography (PET) scanners designed for achieving ultra-high-resolution imaging of small to medium-sized animals as well as the human brain. To ensure peak performance, it is imperative that this module operates consistently at a stable temperature. In this paper, we present an automatic and real-time method to improve the thermal control of the scanner LabPET II by evacuating the heat to reduce the overall temperature and maintain the thermal stability of the system. Based on the Thermo-Electric Cooler (TEC) process, our method applies two numerical techniques, Computational Fluid Dynamics (CFD) and Heat Transfer Analysis (HTA). The Dirichlet Boundary Conditions (DBC) method is applied for the Application-Specific Integrated Circuit (ASIC) at 25∘ C. The real measurements confirm and validate the simulation results with the Finite Element Method (FEM) based on the software COMSOL Multiphysics™. Field-Programmable Gate Array (FPGA) implementation on the Development and Education (DE1) board is performed with comparative results study. The proposed method is both simple and practical, and ensures thermal stability at 35∘C for the duration of operation of the scanner, which has a complex structure.

Radiographic findings in the coxofemoral joint of Australian Cattle Dogs

Australian Cattle Dogs (ACD) are medium-sized animals widely used in fieldwork for managing cattle and sheep. There needs to be more information about the conditions these dogs can develop despite being well-characterized animals since the beginning of the 20th century. Hip dysplasia (HD) is a developmental abnormality between the femoral head and the acetabular fossa, which can be debilitating. However, the available literature has no studies on the prevalence of this condition in dogs of this breed. This study aimed to evaluate radiographs of ACD qualitatively and quantitatively. For this purpose, 49 dogs considered healthy without clinical signs of HD were radiographically assessed, and the animals were classified as dysplastic (D) and non-dysplastic (ND). A frequency of 46.9% of dysplastic dogs was observed, with males being more affected. The cortical index (CI) and angle of inclination (AI) could not differentiate D from ND animals; only the Norberg angle (NA) was effective in this differentiation. No correlation was observed between AI, CI, and AN.

Too good to go? Neanderthal subsistence strategies at Prado Vargas Cave (Burgos, Spain)

Understanding the relationship between Neanderthal groups and their environment and they it is essential to comprehending their ways of life. In this article, we use both zooarchaeology and taphonomy to study the fauna assemblages found in Level 4 of Prado Vargas Cave (Cornejo, Burgos, Spain). The results point to a site in which the main accumulating agent was Neanderthal groups, who transported small- and medium-sized animal carcasses—with deer as the dominant taxon—according to their general utility to systematically and intensely exploit the major muscle bundles, bone marrow, skin, and tendons. According to dental microwear analysis, reiterated and prolonged occupations occurred in diverse moments, suggesting that the site was used most often as a long-term campsite. We detected a minimal amount of carnivore activity, who accessed the cave during periods of human absence and modified some of the remains left by the Neanderthals. Together, this information indicates that Prado Vargas is a key site to understand the dynamics of the Neanderthals in the linking area between the Castillan Plateau and the Cantabrian Range.

Preparation of an Ultrahigh-DAR PDL1 monoclonal antibody-polymeric-SN38 conjugate for precise colon cancer therapy

Antibody-drug conjugates (ADCs) are the most potent active tumor-targeting agents used clinically. However, the preparation of ADCs with high drug-to-antibody ratios (DARs) remains a major challenge. Herein, a Fab-nondestructive SN38-loaded antibody-polymeric-drug conjugate (APDC), aPDL1-NPLG-SN38, was prepared that had a DAR as high as 72 for the first time, by increased numbers of payload binding sites via the carboxyl groups of poly (l-glutamic acid) (PLG). The bonding of Fc-III-4C peptide with PLG-graft-mPEG/SN38 (Fc-NPLG-SN38) was achieved using a click reaction between azide and DBCO groups. The aPDL1-NPLG-SN38 conjugate was then synthesized by the high-affinity interaction between the Fc-III-4C peptide in Fc-NPLG-SN38 and the crystallizable fragment (Fc) of PDL1 monoclonal antibody (aPDL1). This approach avoided the potential deleterious effects on the Fab structure of the monoclonal antibody. The aqueous environment used in its preparation helped maintain monoclonal antibody recognition capability. Through the specific recognition by aPDL1 of PDL1 that is highly expressed on MC38 tumors, the accumulation of aPDL1-NPLG-SN38 in the tumors was 2.8-fold greater than achieved with IgG-NPLG-SN38 that had no active tumor-targeting capability. aPDL1-NPLG-SN38 exhibited excellent therapeutic properties in both medium-sized and large MC38 tumor animal models. The present study provides the details of a novel preparation strategy for SN38-loaded ADCs having a high DAR.

NiTi Shape Memory Clamps with Modified Surface for Bone Fracture Treatment.

Using NiTi alloys with shape memory for long-term medical implants requires modification of their surface due to the possible occurrence of corrosion. Hence, the surface of the staples used to join fractured bone within the craniofacial region was modified by applying a titanium oxy-nitrogen layer and a hydroxyapatite coating. Surface-modified clamps were tested in vivo using New Zealand white rabbits. After determining the mechanical characteristics of the bone and considering the initial state and surface modification, the diameter of the wire (used to make the clamps with the appropriate compression force) was selected. Implantation was performed on two groups of rabbits: experimental and control. In the experimental group, an intentionally induced bone fracture was treated in one tibia. On the second tibia, two additional clamps were applied to increase the possibility of a negative impact of the NiTi alloy on a living organism. After 6 weeks of application, a proper joining of the broken bone fragments was stated. Whereas after twelve weeks, no negative impact of the clamp material on a living organism, i.e., a rabbit, was found. Hence, the clamp with the modified surface can connect bone fragments in humans as well as small and medium-sized animals, with an extended range of use up to 12 weeks.

Hyena and ‘false’ sabre-toothed cat coprolites from the late Middle Miocene of south-eastern Austria

ABSTRACT An association of eighteen coprolites (specimens 01–18) and one isolated coprolite (specimen 209,210) were found in a vertebrate fossil-rich paleosol at the Gratkorn site (south-eastern Austria; late Middle Miocene). The specimens consist mostly of calcium phosphate (apatite) and a matrix formed by microglobules. Coprolites 01–18 show cylindrical and spherical morphologies and are considerably smaller than the tube-shaped specimen 209,210, in which no inclusions were observed. In contrast, coprolites 01–18 contain numerous, highly altered bone fragments (sub-mm-sized long bones and several mm-sized trabecular bone remains) as well as hair imprints, plant detritus and palynomorphs. Based on composition, morphology, size, microstructure, and inclusions, and considering the body fossil record of this site, we assume the hyaenid Protictitherium and the barbourofelid Albanosmilus, as producers of coprolites 01–18 and 209,210, respectively. The preserved bone remains in specimens 01–18 suggest that Protictitherium fed on small vertebrates, but possibly also cracked bones of medium-sized animals. The hair imprints found were either from the hyaenid itself or its prey, while the plant material was probably ingested accidentally. The lack of inclusions in specimen 209,210 is related to the presumably hypercarnivorous diet of Albanosmilus, which was certainly the apex predator in this biome.

Hydrodynamics of a swimming batoid fish at Reynolds numbers up to 148 000

Flow around a tethered model of a swimming batoid fish is studied by using the wall-modelled large-eddy simulation in conjunction with the immersed boundary method. A Reynolds number ( $Re$ ) up to 148 000 is chosen, and it is comparable to that of a medium-sized aquatic animal in cruising swimming state. At such a high $Re$ , we provide, to the best of our knowledge, the first evidence of hairpin vortical (HV) structures near the body surface using three-dimensional high-fidelity flow field data. It is observed that such small-scale vortical structures are mainly formed through two mechanisms: the leading-edge vortex (LEV)–secondary filament–HV and LEV–HV transformations in different regions. The HVs create strong fluctuations in the pressure distribution and frequency spectrum. Simulations are also conducted at $Re=1480$ and 14 800 to reveal the effect of Reynolds number. Variations of the flow separation behaviour and local pressure with $Re$ are presented. Our results indicate that low- $Re$ simulations are meaningful when the focus is on the force variation tendency, whereas high- $Re$ simulations are needed when concerning flow fluctuations and turbulence mechanisms.

Increasing Detections of Small to Medium-Sized Mammals Using Multiple Game Cameras

Surveys using game cameras have become a standard method to determine presence of small to medium-sized mammals such as Spilogale putorius (Eastern Spotted Skunk). Surveys typically use a single camera at each site; however, prior work with other species suggests that single cameras are less likely than multiple cameras to detect small and mid-size mammals. During the winters of 2019–2020 and 2020–2021, we placed 71 camera stations in the Arkansas Ozarks, with each station having 2 cameras aimed at the same target. We used detections of a common species, Sciurus carolinensis (Eastern Gray Squirrel), to determine if adding an additional camera at each site could increase detections of animals similar in size. Overall, detections increased 27% with the addition of a second camera. We found that a second camera increased Eastern Gray Squirrel detections by 15.6% when the animal was closer than 1 body-length from the target bait tree and 41.2% when the animal was more than 1 body-length from the bait tree. We conclude that a single camera may miss a significant number of detections of small to medium-sized animals and could bias results of detection/non-detection studies.

Uso do Alphitobius diaperinus como método alternativo de osteotécnica

Abstract This study aimed to develop an alternative biological osteotechnical method using lesser mealworms (Alphitobius diaperinus), a scavenging insect found in poultry production systems. Ten small to medium-sized animal cadavers of different species and two isolated bone pieces were used. After the soft tissues were removed, the bones were accommodated with the insects until they were thoroughly cleaned. Next, the pieces were evaluated, bleached, dried, and the skeletons assembled. The use of lesser mealworms in the biological preparation of skeletons has shown very promising results since this insect is easy to acquire, maintain, and handle; it does not emit unpleasant odors into the environment; it provides complete cleaning of the skeletons, reaching places that are difficult to access. In addition, they present great agility in cleaning the carcasses, dispensing the previous fixation of the soft tissues to prevent putrefaction or the appearance of microorganisms, such as fungal colonies.

Development of a depuration protocol for commercially important edible bivalve molluscs of India: Ensuring microbiological safety

We investigated the effect of depuration of three naturally contaminated commercially important tropical edible bivalve molluscs by varying temperature, salinity and body-size of animals harvested from Ashtamudi and Vembanad estuaries, India using a static depuration system to ensure microbiological food safety. Before depuration, the levels of faecal indicators and pathogens were above the acceptable limits for live consumption. The depuration water temperature had a significant effect on bacterial elimination. Log reduction of faecal coliforms (FC) and E. coli varied between room-temperature (RTDS) and low-temperature depuration system (LTDS) and it was in the range of 1.39–2.44 and 1.88–2.82 log MPN, respectively under RTDS and LTDS. The elimination of bacterial pathogens such as Vibrio and Salmonella spp. was rapid in RTDS compared to LTDS. The highest elimination of FC and E. coli (2.39 and 2.92 log) was at 35 psμ depuration and the lowest (0.87 and 1.65 log) at 15 psμ depuration. The reduction of FC and E. coli was higher in the medium-sized animals compared to the small animals. Based on the results it is recommended that depuration using the static system under room-temperature (30 ± 1 °C); a salinity range of (25–35 psμ) and using medium-size bivalves (clam>30 mm; mussel >45 mm, and oyster >65 mm length) as optimum conditions for producing microbiologically safe bivalves for live consumption within 36 h.

Nuevo registro de Puma concolor Linnaeus, 1771 (Carnivora, Felidae) para el Pleistoceno Tardío de Uruguay y su importancia paleoecológica

Felidae entered South America from North America during the Ensenadan Stage/Age (Early to Middle Pleistocene). For Uruguay their fossil record is scarce but informative, although mostly correspond to large felids (Smilodon, Panthera onca). In this work describes fossil remain of a skull and a mandible assigned, based on morphology and statistical analysis, to Puma concolor. These materials were unearthed from Late Pleistocene sediments (Sopas Formation) from northern Uruguay. Based on these remains some paleobiological and
 paleoecological aspects are discussed. This specimen had a body mass of about 45 kg, with the potential capacity to hunt animals over 200 kg, which is in agreement with previous reports for other fossil puma remains. During Late Pleistocene, in the current territory of Uruguay, Puma concolor would have shared ecological niche with other large carnivore such as sabre-tooth cats (Smilodon populator, S. fatalis), the jaguar Panthera onca, the canid Protocyon troglodytes, and short-faced bears Arctotherium, feeding upon medium-sized animals in diverse environment.

Nomination of a hunting object in the Komi language

Introduction. The paper deals with two components of the hunting vocabulary, namely large wild animals, and medium-sized forest dwellers. Each component sub-defines the nominative units related to predators and herbivores are distinguished and separately considered in the paper. In the first part of the study, the composition is determined and a brief description of the relevant representatives of the local commercial fauna in the Komi Territory is given. The second part presents a diachronic hierarchy of the analyzed primordial vocabulary, compiled on the basis of the classification of proto-linguistic reconstructions. Materials and Methods. The analyzed material refers mainly to the Komi literary language. The paper considers the names of both native and foreign-language origin. The main methods used by the author in developing the research topic are descriptive, comparative-historical, synchronous-comparative and statistical. Results and Discussion. For the first time the article presents a linguistic analysis using the biological classification of two lexical subgroups belonging to one of the microsystems of the hunting vocabulary in the Komi language. It was established that the object of the study has ancient origins and the designations of primordial origin make its basis. In the composition of this variety of hunting vocabulary it presents Proto-Finno-Ugric, Proto-Finno-Permian, Proto-Permian and Proto-Komi vocabulary. A group of late formations having no genetic correspondences in other related languages appeared in the period of independent existence of the Komi-Zyryan language, after its separation from the Komi-Permian language. The foreign language component belongs to early and late borrowings. The technology of developing this topic, created by the author of the paper, can be used in the material of other related and unrelated languages. Conclusion. The formation and development of the Komi language vocabulary related to large commercially hunted animals and medium-sized wild animals has been going on for many millennia, since the Proto-Finno-Ugric epoch. Most of the names of the analyzed microsystem belong to the primordial vocabulary fund of the Komi language. A foreign-language component consisting of borrowings from 5 external sources took part in its replenishment.

The Presence of Mycobacterium leprae in Wild Rodents.

Leprosy is a chronic infection caused by Mycobacterium leprae. There is a lack of data regarding environmental reservoirs, which may represent a serious public health problem in Brazil, especially in the state of Pará, which occupies the fourth position in incidence of cases in the country. Previous studies report evidence of infection occurring among armadillos, mangabei monkeys, and chimpanzees. In the present study, wild animals were captured and tested for the presence of anti-PGL-1 antibodies and M. leprae DNA. Fieldwork was carried out from October to November of 2016 in the cities of Curionópolis and Canaã dos Carajás, southeast of Pará state. Small and medium-sized wild animals were captured using appropriate traps. A total of 15 animals were captured. Sera and viscera fragments were collected and tested by ELISA and PCR methods. The presence of M. leprae DNA was confirmed by sequencing of specific gyrase gene in three animals of two different species, including one Necromys lasiurus (liver sample) and two Proechimys roberti (kidney and liver samples). This unprecedented finding suggests that species other than those previously reported are responsible for maintaining M. leprae in nature.

Bathing Behaviour in Spotted Hyenas (Crocuta crocuta) in Laikipia, Kenya: Two Observational Cases

Homeothermic animals have regulatory mechanisms to maintain a constant body temperature in response to harsh climatic conditions. In low latitude areas near the equator, animals have adapted a coat to avoid strong UV rays, but a thick coat interferes with heat dissipation due to high daytime temperatures. Therefore, animals spend hot hours in cool shade or dens as the most common method to avoid overheating Alternatively, some animals cool themselves by bathing with sand, mud or water. Spotted hyenas (Crocuta crocuta) are medium-sized carnivorous animals weighing about 45–70 kg, and aredouble coated. Bathing is a well-known behaviour in spotted hyenas, but it is not clear from natural historical descriptions whether their bathing is a heat-resistant behaviour. Here we report on two bathing observation cases of hyena and describe them with reference to the heat resistance function of bathing based on the time and temperature at which observations occurred. A wild hyena of a four-headed clan inhabiting Laikipia, Kenya bathed in a pool at around 18:00 h and 9:00 h in the dry season. The temperature at 18:00 h showed largest difference from the highest annual mean temperature. The bathing at 9:00 h in the morning was the time when the temperature began to rise. These observations suggest that hyena bathing may be a heat-avoiding behaviour. Bathing also has other functions such as parasite extermination, play and ambush hunting, hence more observations are needed in the future.

Performance Evaluation of a Preclinical SPECT Scanner with a Collimator Designed for Medium-Sized Animals.

Background Equipped with two stationary detectors, a large bore collimator for medium-sized animals has been recently introduced for dedicated preclinical single-photon emission computed tomography (SPECT) imaging. We aimed to evaluate the basic performance of the system using phantoms and healthy rabbits. Methods A general-purpose medium-sized animal (GP-MSA) collimator with 135 mm bore diameter and thirty-three holes of 2.5 mm diameter was installed on an ultrahigh-resolution scanner equipped with two large stationary detectors (U-SPECT5-E/CT). The sensitivity and uniformity were investigated using a point source and a cylinder phantom containing 99mTc-pertechnetate, respectively. Uniformity (in %) was derived using volumes of interest (VOIs) on images of the cylinder phantom and calculated as [(maximum count − minimum count)/(maximum count + minimum count) × 100], with lower values of % indicating superior performance. The spatial resolution and contrast-to-noise ratios (CNRs) were evaluated with images of a hot-rod Derenzo phantom using different activity concentrations. Feasibility of in vivo SPECT imaging was finally confirmed by rabbit imaging with the most commonly used clinical myocardial perfusion SPECT agent [99mTc]Tc-sestamibi (dynamic acquisition with a scan time of 5 min). Results In the performance evaluation, a sensitivity of 790 cps/MBq, a spatial resolution with the hot-rod phantom of 2.5 mm, and a uniformity of 39.2% were achieved. The CNRs of the rod size 2.5 mm were 1.37, 1.24, 1.20, and 0.85 for activity concentration of 29.2, 1.0, 0.5, and 0.1 MBq/mL, respectively. Dynamic SPECT imaging in rabbits allowed to visualize most of the thorax and to generate time-activity curves of the left myocardial wall and ventricular cavity. Conclusion Preclinical U-SPECT5-E/CT equipped with a large bore collimator demonstrated adequate sensitivity and resolution for in vivo rabbit imaging. Along with its unique features of SPECT molecular functional imaging is a superior collimator technology that is applicable to medium-sized animal models and thus may promote translational research for diagnostic purposes and development of novel therapeutics.