Mediterranean Type Research Articles

The Effect of Dietary Types on Gut Microbiota Composition and Development of Non-Communicable Diseases: A Narrative Review.

The importance of diet in shaping the gut microbiota is well established and may help improve an individual's overall health. Many other factors, such as genetics, age, exercise, antibiotic therapy, or tobacco use, also play a role in influencing gut microbiota. This narrative review summarizes how three distinct dietary types (plant-based, Mediterranean, and Western) affect the composition of gut microbiota and the development of non-communicable diseases (NCDs). A comprehensive literature search was conducted using the PubMed, Web of Science, and Scopus databases, focusing on the keywords "dietary pattern", "gut microbiota" and "dysbiosis". Both plant-based and Mediterranean diets have been shown to promote the production of beneficial bacterial metabolites, such as short-chain fatty acids (SCFAs), while simultaneously lowering concentrations of trimethylamine-N-oxide (TMAO), a molecule associated with negative health outcomes. Additionally, they have a positive impact on microbial diversity and therefore are generally considered healthy dietary types. On the other hand, the Western diet is a typical example of an unhealthy nutritional approach leading to an overgrowth of pathogenic bacteria, where TMAO levels rise and SCFA production drops due to gut dysbiosis. The current scientific literature consistently highlights the superiority of plant-based and Mediterranean dietary types over the Western diet in promoting gut health and preventing NCDs. Understanding the influence of diet on gut microbiota modulation may pave the way for novel therapeutic strategies.

Short-term soil fungal community dynamics following fire in mediterranean climate-type banksia woodlands

Fire is a dominant ecosystem process in many Mediterranean climate type ecosystems, and is predicted to increase in severity and frequency, shifting away from previous regimes in many regions. Responses of flora and fauna to fire are relatively well studied, but less is known about the responses of belowground microbiota. We quantified soil fungal dynamics over the first 12–15 months after fire, focusing on attributes of the fire regime (season, interval, severity). Soil samples were collected from three sites in a threatened woodland ecosystem in southwestern Australia, a Mediterranean-type climate region. Fungal taxa were identified via high throughput sequencing of the ITS subregion and taxonomy assigned using reference databases. Richness, diversity, abundance, community composition, and functional groups were quantified. Over the post-fire sampling period, richness and diversity declined and soil fungal community composition changed significantly throughout the sampling period, with family level taxa and functional groupings experiencing the most change. Through the sampling period, an increase in saprotrophic and endophytic fungi was observed, along with a decrease in all pathogenic fungi. We found that the post-fire fungal community is quite dynamic in the first 12–15 months after fire. We found little effect of fire interval or fire season, though our inference was limited. Our work contributes to putting belowground biota into the same conceptual frameworks as aboveground taxa and serves to inform fire managers in fire-prone Mediterranean climate type regions.

Unveiling the Opportunities of Unexplored Use of Cover Crop in Mediterranean Agriculture through Systematic Review and Meta-Analysis

Cover crops are multifunctional, and contribute to improving soil properties and reducing environmental impact compared to no-cover crops, thus could provide multiple soil, agricultural, and environmental benefits, and they are recognized as a valid strategy for the achievement of sustainable agriculture. However, cover crops’ impacts on subsequent cash crops and soil characteristics are dependent on several factors, such as pedoclimatic conditions, cover crop species, agricultural practices, method of termination, and irrigation management. The fact that cover crops are never applied as a single practice in the real agricultural sector, but are instead combined with other factors or agricultural practices, deeply affects their performance, but the scientific literature nevertheless keeps considering the use of cover crops alone. Moreover, the potential outcomes of cover crops that encompass other factors or agricultural practices affecting soil quality, weed control, and cash crops are still unclear. Additionally, cover crops are still poorly use in the Mediterranean type of climate. Therefore, this study reviewed the scientific literature to identify the most relevant factors or agricultural practices driving cover crops’ performance, and to outline future fields of investigation looking towards promising sustainable agriculture in Mediterranean areas with a view to minimizing the competition for soil water with the cropping systems and to reduce soil degradation. Furthermore, the investigation includes multiple factors or agricultural practices that better represent the real farming system, contributing to a comprehensive understanding of their interactions with cover crops, and suggests alternative strategies for reducing yield gap while seeking to achieve agricultural sustainability.

Changes in Population Densities and Species Richness of Pollinators in the Carpathian Basin during the Last 50 Years (Hymenoptera, Diptera, Lepidoptera)

Temporal changes in population densities and species richness of three main pollinator groups—moths and butterflies (Lepidoptera); bees, wasps and sawflies (Hymenoptera); and hoverflies, horseflies, tachinids and bee flies (Diptera)—were investigated in the Carpathian Basin. Maintaining pollinator diversity is a crucial factor for preserving our biodiversity and ecosystems; furthermore, several pollinator species have a strong economic role in maintaining crop and fruit cultures. Our conclusions are based on our three and four decades of faunistic surveys in various regions of the Carpathian Basin. Analyzing and comparing our data with the historical data of the last 50 years, we concluded that densities of some pollinators declined during the past decade and a half (Symphyta, hoverflies), although populations of several species of Mediterranean origin grew (Aculeata) and new species even migrated from the warmer regions. In numerous cases, this decrease was dramatic: more than 90% decline of certain butterfly species were detected. On the other hand, the composition of pollinator fauna significantly changed due to the disappearance of some mountainous or mesophile species. The main reason for the decrease in pollinator communities is due partly to climatic change and partly to anthropogenic factors. Different groups of pollinators react differently: some groups like Syrphidae, Tachinidae, most of the butterfly families and bumblebees suffered a strong decline in the last two decades; other warm-loving groups like most of Aculeata and horseflies and bee flies showed a significant increase in population densities. Our conclusion: in our region, the pollinator crisis is present but moderate; however, there is a clear sign of the gradual transition of our pollinator fauna towards the Mediterranean type.

Variable role of diet-related risk factors for colorectal cancer – A cross-sectional analysis of regional differences

Background and objectives. Diet is the crucial environmental factor in sporadic colorectal cancer. Its complexity and diversity reflect our cultural and historical background, and could potentially explain why the existing public health strategies focused on colorectal cancer are failing. Materials and methods. An observational cross-sectional study encompassed 60 non-metastatic CRC patients to assess dietary factors related to colorectal cancer, with regard to their traditional diet i.e. the region they come from. Patients were recruited from two regions (30 patients per region), in one (Slavonia) traditional diet is abundant in well-known dietary risk factors for colorectal cancer, and in the second (Dalmatia) traditional diet is the Mediterranean type. Results. Based on the logistic regression analysis, patients from Dalmatia have a 24% higher risk for the high-risk diet in comparison to patients from Slavonia (OR = 1.240, 95% CI 1.195 – 9.990, P=0.022). Identified independent risk factors include weight loss in the last 3 months along with daily alcohol consumption which were found to increase CRC risk by 61.4% and 53.1%, respectively. Conclusions. Our results support statistical data showing that colorectal cancer incidence is higher in the Mediterranean region, suggesting a shift in the Mediterranean dietary pattern, which in our case were higher obesity rates, daily alcohol consumption and abundance of unfavorable dietary habits.

Activities of antioxidant enzymes and Hsp levels in response to elevated temperature in land snail species with varied latitudinal distribution.

Land snails occupy a variety of habitats, with differing temperature and humidity regimes and exhibit a wide span of adaptations, to withstand abiotic condition changes. The present work's aim was to examine the correlation of habitat's thermal adversity in different Mediterranean type habitats with the land snail's antioxidant and heat shock responses. For this purpose, snails of different species from populations along a north-south axis from the islands and mainland of Greece were exposed to elevated temperature and antioxidant enzyme activities, and Hsp70 and Hsp90 levels were determined in their tissues. The ATP, ADP, and AMP levels and the adenylate energy charge (AEC) were also determined. The comparison of protein levels and enzymatic activities across time intervals revealed significant differences for all factors examined. While the gradation pattern over time for a given factor was similar in all populations the absolute values over time differed. Catalase activity and the Hsp90 protein levels had the higher contribution in separating the different species and populations, followed by the activity of glutathione reductase and Hsp70 protein levels which contributed to a lesser degree. In general, populations from the southern part of their distribution in Greece tend to display a faster increase than northern populations in induction levels of all factors examined. Our data seem to be in line with the concept of preparation for oxidative stress (POS) while the changes in the AEC indicate an early preparation to cover the energy demand for the induction and synthesis of antioxidant enzymes and Hsps.

Features of the Development of Tropical Crops in the Conditions of Dry Subtropics of the Southern Coast of the Crimea

Aerva lanata (L.) Juss. and Passiflora incarnata L. are pharmacopoeial medicinal plants, the raw materials of which are included in the list of imported to the Russian Federation. We conducted the complex of ecological and biological studies in order to select the optimal method of culture (long-term or annual) of these crops and assess the prospects of their cultivation in the conditions of the Southern Coast of the Crimea. In accordance with the methods of introduction study, we revealed the features of biology, growth dynamics and development of plants Aerva lanata and Passiflora incarnata in a dry subtropical climate of the Mediterranean type. It was found that for the cultivation of Passiflora incarnata for the long-term type, the required heat resource higher than 10˚С is 2159˚С. Aerva lanata in the open ground of the annual type forms fully valid seeds with heat supply in the 1870˚С. The biologically necessary amount of heat of the open ground culture is provided by the average long-term effective temperatures for Passiflora incarnata at 97% and Aerva lanata – 98-100%. These species are classified as promising species based on the total number of points.

The update and ecological status of the flora of the Belezma National Park, Algeria

Abstract. Benchouala A, Bekdouche F, Si Bachir A. 2023. The update and ecological status of the flora of the Belezma National Park, Algeria. Biodiversitas 24: 6134-6150. The establishment of national parks and nature-protected areas helps to conserve biodiversity. The conservation and enhancement of the floristic diversity of the Belezma National Park (BNP), Algeria which is also designated as biosphere reserve, require precise knowledge of its flora. To that end, the inventory conducted at BNP at the level of forest massifs, preforests, and mountain grasslands enabled us to identify 562 taxa belonging to 342 genera and 93 families, with a predominance of Asteraceae, Poaceae, and Fabaceae. Based on biological spectrum, hemicryptophytes outnumbers other life forms by 44.4%. Zoochory is the most common distribution mode among inventoried species (44.3%). The Mediterranean type dominates the chronological spectrum with proportion of 45.9%. Endemism is represented by 53 species (9.4% of the total flora). According to executive decree No. 12-03 of January 4, 2012, the BNP flora consists of 196 rare species, including 34 endemic species and 26 threatened and protected ones in Algeria. Based on our results, it has been possible to constitute a database of the BNP flora, useful for its protection.

The admixture of Quercus sp. in Pinus sylvestris stands influences wood anatomical trait responses to climatic variability and drought events.

Forests are threatened by increasingly severe and more frequent drought events worldwide. Mono-specific forests, developed as a consequence of widespread management practices established early last century, seem particularly susceptible to global warming and drought compared with mixed-species forests. Although, in several contexts, mixed-species forests display higher species diversity, higher productivity, and higher resilience, previous studies highlighted contrasting findings, with not only many positive but also neutral or negative effects on tree performance that could be related to tree species diversity. Processes underlying this relationship need to be investigated. Wood anatomical traits are informative proxies of tree functioning, and they can potentially provide novel long-term insights in this regard. However, wood anatomical traits are critically understudied in such a context. Here, we assess the role of tree admixture on Pinus sylvestris L. xylem traits such as mean hydraulic diameter, cell wall thickness, and anatomical wood density, and we test the variability of these traits in response to climatic parameters such as temperature, precipitation, and drought event frequency and intensity. Three monocultural plots of P. sylvestris and three mixed-stand plots of P. sylvestris and Quercus sp. were identified in Poland and Spain, representing Continental and Mediterranean climate types, respectively. In each plot, we analyzed xylem traits from three P. sylvestris trees, for a total of nine trees in monocultures and nine in mixed stands per study location. The results highlighted that anatomical wood density was one of the most sensitive traits to detect tree responses to climatic conditions and drought under different climate and forest types. Inter-specific facilitation mechanisms were detected in the admixture between P. sylvestris and Quercus sp., especially during the early growing season and during stressful events such as spring droughts, although they had negligible effects in the late growing season. Our findings suggest that the admixture between P. sylvestris and Quercus sp. increases the resilience of P. sylvestris to extreme droughts. In a global warming scenario, this admixture could represent a useful adaptive management option.

Greater fruit and vegetables consumption, and adherence to a Mediterranean type of diet reduces the risk for asthma in children; a systematic review and meta-analysis

The objective of this study was to explore the relationship between the consumption of fruits, vegetables and adherence to the Mediterranean diet with childhood asthma development and severity. A systematic literature search and synthesis of the results was performed throughout the last two decades. A total of 45 studies were analysed and 392,797 children were included. Greater adherence to a Mediterranean type of diet was inversely associated with asthmatic outcomes (OR:0.71,95% CI:0.54,0.88). Regarding fruits, vegetables consumption, a significant inverse association between increased fruits or vegetable intake and asthma was found (OR:0.82,95% CI:0.77,0.86; 0.84,95% CI:0.77,0.91, respectively). Fruits and vegetables consumption combined was also inversely related to all asthmatic outcomes (OR:0.65,95% CI:0.49,0.78). The level of heterogeneity was moderate-to-high (30%–97%). The present review and meta-analysis show a trend to an inverse association between adherence to the Mediterranean diet or a healthy diet rich in fruits and vegetables and the occurrence of childhood asthma but with a low level of certainty.

SPECIES DIVERSITY AND STRUCTURE OF THE SAXICOLOUS FLORAL COMPLEX IN THE AKTOBE FLORISTIC DISTRICT

The study of the Aktobe floristic district is a relevant task for assessing anthropogenic factors’ impacts on the flora of the steppe zone and developing measures to reduce the adverse effects of mining, metallurgical, and oil and gas facilities on the biodiversity of plant communities. The timely study evaluated the species composition and analyzed the saxicolous floral complex of the Aktobe floristic district, one of the most fascinating complexes based on florogenetics. The saxicolous complex flora comprised 219 species belonging to 119 genera and 39 families. However, the 10 leading families of the conglomerate manage 75.0% of the species composition of the saxicolous complex. Previous validations revealed that the saxicolous floral complex features have dominant families of Caryophyllaceae, Boraginaceae, and Chenopodiaceae and a more significant role for the Rosaceae and Scrophulariaceae. The saxicolous complex specificity has demonstrated the genera Potentilla and Centaurea as 2nd and 3rd position holders, respectively, and the presence of the genera Orobanche, Lappula, Atraphaxis, and Allium as the leading genera; however, they are unregistered among the other floral complexes. The analysis of geographical habitat types has shown that the Black Sea/Kazakhstan, Volga/Kazakhstan, and Eastern ancient Mediterranean habitat types are predominantly characteristic of the saxicolous floral complex. Twelve endemic species have also received recognition, assembling 60% of the endemic flora. Based on the analysis of species habitat, its ecological and biological features, and paleobotanical material available in literature sources, researchers have identified two groups of miscellaneous plants (Neogene and Quaternary) with their ages and endemic plants. Data also indicated the formation of the chief autochthonous core of the flora in the Pleiocene-Holocene.

Food Compass Score predicts incident cardiovascular disease: The ATTICA cohort study (2002-2022).

The Food Compass Score (FCS) is a novel nutrient profiling system, which evaluates food and diet quality. The present study aimed to prospectively assess the relationship of FCS with short-term (10 years) and long-term (20 years) cardiovascular disease (CVD) incidence and to explore whether this relationship is modified by long-term adherence to a Mediterranean type diet (MTD). Volunteers of the ATTICA cohort study, with complete data for the calculation of FCS and incident CVD were included (n = 759). Development of CVD was determined at 10 and 20 years after baseline. Dietary intake was assessed through a validated food frequency questionnaire. The FCS was calculated for each participant based on the published algorithm. Long-term adherence to a MTD was evaluated through MedDietScore. FCS was inversely associated with CVD incidence (hazard ratio [HR] for 20-year follow-up = 0.97, 95% confidence interval [CI] = 0.95-0.99; HR for 10-year follow-up = 0.98, 95% CI = 0.96-1.01) in the total sample, as well as in those with a high baseline adherence to a MTD (HR for 20-year follow-up = 0.96, 95% CI = 0.93-0.99; HR for 10-year follow-up = 0.98, 95% CI = 0.95-1.02). FCS was also inversely associated with CVD risk in those who went away from the MTD (HR = 0.97, 95% CI = 0.96-0.99). FCS, a novel tool for assessing overall diet quality, was also found to be useful in identifying potential CVD candidates in a long-term period, even in populations with good background dietary habits, such as those following a MTD.

Association between a mediterranean lifestyle and Type 2 diabetes incidence: a prospective UK biobank study

BackgroundThere is mounting evidence that the Mediterranean diet prevents type 2 diabetes, but little is known about the role of Mediterranean lifestyles other than diet and among non-Mediterranean populations. This work aimed to examine the association between a comprehensive Mediterranean-type lifestyle and type 2 diabetes incidence in a British adult population.MethodsWe used data from 112,493 individuals free of cardiovascular disease and type 2 diabetes mellitus, aged 40–69 years, from the UK Biobank cohort, who were followed from 2009 to 2010 to 2021. The Mediterranean lifestyle was assessed through the 25-item MEDLIFE index, which comprises three blocks: (a) “Mediterranean food consumption”, (b) “Mediterranean dietary habits”, (c) “Physical activity, rest, social habits, and conviviality”. Diabetes incidence was obtained from clinical records. Cox proportional-hazards regression models were used to analyze associations and adjusted for the main potential confounders.ResultsAfter a median follow-up of 9.4 years, 2,724 cases of type 2 diabetes were ascertained. Compared to the first quartile of MEDLIFE adherence, the hazard ratios (95% confidence interval) for increasing quartiles of adherence were 0.90 (0.82–0.99), 0.80 (0.72–0.89) and 0.70 (0.62–0.79) (p-trend < 0.001). All three blocks of MEDLIFE were independently associated with lower risk of diabetes.ConclusionsHigher adherence to the MEDLIFE index was associated with lower risk of type 2 diabetes in the UK Biobank. A Mediterranean-type lifestyle, culturally adapted to non-Mediterranean populations, could help prevent diabetes.

Yearly attained adherence to Mediterranean diet and incidence of diabetes in a large randomized trial

BackgroundSeveral large observational prospective studies have reported a protection by the traditional Mediterranean diet against type 2 diabetes, but none of them used yearly repeated measures of dietary intake. Repeated measurements of dietary intake are able to improve subject classification and to increase the quality of the assessed relationships in nutritional epidemiology. Beyond observational studies, randomized trials provide stronger causal evidence. In the context of a randomized trial of primary cardiovascular prevention, we assessed type 2 diabetes incidence according to yearly repeated measures of compliance with a nutritional intervention based on the traditional Mediterranean diet.MethodsPREDIMED (‘‘PREvención con DIeta MEDiterránea’’) was a Spanish trial including 7447 men and women at high cardiovascular risk. We assessed 3541 participants initially free of diabetes and originally randomized to 1 of 3 diets: low-fat diet (n = 1147, control group), Mediterranean diet supplemented with extra virgin olive (n = 1154) or Mediterranean diet supplemented with mixed nuts (n = 1240). As exposure we used actual adherence to Mediterranean diet (cumulative average), yearly assessed with the Mediterranean Diet Adherence Screener (scoring 0 to 14 points), and repeated up to 8 times (baseline and 7 consecutive follow-up years). This score was categorized into four groups: < 8, 8–< 10, 10– < 12, and 12–14 points. The outcome was new-onset type 2 diabetes.ResultsMultivariable-adjusted hazard ratios from time-varying Cox models were 0.80 (95% confidence interval, 0.70–0.92) per + 2 points in Mediterranean Diet Adherence Screener (linear trend p = .001), and 0.46 (0.25–0.83) for the highest (12–14 points) versus the lowest (< 8) adherence. This inverse association was maintained after additionally adjusting for the randomized arm. Age- and sex-adjusted analysis of a validated plasma metabolomic signature of the Mediterranean Diet Adherence Screener (constituted of 67 metabolites) in a subset of 889 participants also supported these results.ConclusionsDietary intervention trials should quantify actual dietary adherence throughout the trial period to enhance the benefits and to assist results interpretation. A rapid dietary assessment tool, yearly repeated as a screener, was able to capture a strong inverse linear relationship between Mediterranean diet and type 2 diabetes.Trial registration ISRCTN35739639

Viking Age Windows

In the last 25 years a conspicuous amount of plane glass – windowpane fragments – has surfaced on archaeological sites from the Viking Age. These finds have not received scholarly attention as they are not recognised as a genuine prehistoric (i.e. pre-1050 Scandinavia) occurrence. This paper aims to investigate a select group of archaeological localities that all have a significant amount of glass objects and fragments, and which also serve as mainstays for continental influences, commercial trade, as well as ritual activities. It offers the study of the chemical composition of these windowpane fragments, their distribution, provenience, and discusses their potential use as windows in Viking Age Scandinavia. Based on the chemical composition of the analysed plane glass (via LA-ICP-MS) the paper argues, firstly, that the glass most likely should be dated to the 9th to 11th centuries; secondly, that there are two possible import paths of raw material with one recognized at the early emporia based on east Mediterranean types of glass, and another with a continental type of glass found at the aristocratic sites. Finally, the paper argues that the windowpanes very likely could have been used in contemporary glassed windows placed in wooden buildings at these sites.

Effect of Mediterranean Dietary Pattern on Cognitive Status in Community-Dwelling Older Adults.

Modifiable factors associated with cognitive decline (CD) require more attention, particularly dietary patterns. This study aimed to investigate the link between cognitive decline and associated factors, particularly dietary patterns (DPs), in community-dwelling older Lebanese of modest economic status. Our cross-sectional national study included 352 participants above 60 years old, from the medico-social centers of the ministry of social affairs all over the country. CD was screened based on literacy. Nutritional and dietary data were collected through a validated food frequency questionnaire. DPs were extracted by the K-mean cluster analysis. CD was found in 32.7% and 61.5% of literate and illiterate groups, respectively. Identified DPs included a Westernized type and Mediterranean type, with high and moderate food intakes. In the context of literacy, independent factors associated with CD were age above 80 years, living in Beirut, frailty, and adopting a Westernized (OR = 3.08, 95% CI: 1.22-7.8) and a high-intake Mediterranean DP (OR = 2.11, 95% CI: 1.05-4.22). In the context of illiteracy, the same factors were associated with CD, but not DP nor frailty, with an age cut-off at 78 years. In a Lebanese sample of older adults, factors associated with CD depend on the level of literacy, with DP only associated with CD in the context of literacy.

Growth And Production of Lowland Rice Due to Soil Ameliorant Application on Andosol, Mediterranean And Alluvial Soil

Lowland rice production and growth in Andosol, Mediterranean, and Alluvial soil types were observed in the study, both with and without amendments. The investigation was conducted using a plot experiment with a fully randomized design and three replications. L1: Andosol, L2: Mediterranean, and L3: Alluvial soil types made up the primary plots. Land amendments were divided into two subplots: R1 with no amendment and R2 with an amendment. Plant height, shoot and root dry weight, shoot:root ratio, and grain dry weight were the parameters measured. The findings demonstrated a significant (P<0.05) impact of the interaction effect of soil type amendment and bioslurry dose on the development and yield of three rice cultivars. Plant height, shoot dry weight, and grain dry weight with soil amendment were more significant (P<0.05) than without amendment, according to the interaction between soil type and amendment. The amendment application had little effect on the andosol soil. Alluvial soil types had the highest soil amendment response (P<0.05), followed by Mediterranean and Andosols. Based on the experimental findings, the soil enhancer formulation has been suggested for use in rice production, particularly in Mediterranean and Alluvial soils, although additional study is required to develop new formulations for Andosol soils.

Межгрупповая изменчивость краниометрических характеристик населения Средней Азии в 1500-летней ретроспективе (VII в. до н.э. – VIII в. н.э.)

Introduction. The paper revisits the intergroup craniometric variability in the Early Iron Age, Antiquity and Early medieval Central Asian population. Some new cranial materials are considered within the hierarchical system of the regional populations. Materials and methods. The comparison included 61 cranial samples from all historical and cultural regions of Central Asia with a total of 1100 crania. 12 standard measurements of the facial skeleton were used. First, we conducted Canonical discriminant analysis. Second, the matrix of Mahalanobis D2 distances was obtained using the resulting group coordinates in the space of the canonical vectors. Finally, the distance matrix was subjected to Multidimensional scaling and the Ward’s hierarchical cluster analysis. Results. The first three canonical variates reflect 53.4% of the total intergroup variation and differentiate samples based on bizygomatic breadth, orbit dimensions, nasal bridge height (and width to a lesser extent), horizontal profiling angles and nose protrusion angle. Upper facial height also contributes to intergroup craniometric variability but does not play a decisive role in differentiating among samples of European ancestry in the considered chronological period. Correlation coefficients between canonical variables and multidimensional scaling coordinates suggest similar relative positions of the groups in the scatterplots. Discussion. Considering the mixed nature of the Central Asian population and the difficulty of objective differentiation of morphological types, we caution against unreflective use of terms “East Mediterranean type” and “Pamir-Fergana race”. The results suggest that the Antiquity and Early medieval sedentary populations are difficult to distinguish from nomadic and semi-nomadic groups of the Wusun period. The new materials confirm the morphological similarity between Early medieval population of Sogdiana (ancient Panjakent) and Ushrusana (Kurkat dakhmaks). @ 2023. This work is licensed under a CC BY 4.0 license

Estimation of daily global solar radiation based on different whitening applications using temperature in Mediterranean type greenhouses

The study aimed to estimate the daily global solar radiation (Rs) in Mediterranean-type greenhouses. Five different temperature-based Rs estimation models developed for open-field conditions were calibrated and validated in Mediterranean-type greenhouses in Almeria, Spain and Antalya, Türkiye, between August 26, 2013, and January 1, 2023, and between October 1, 2018, and 1 January 2023, respectively. Whitening applications were categorized according to greenhouse light transmissivity and classified as follows: without whitening or light-whitening, medium-whitening, and severe-whitening. Additionally, the best-performing model were compared with greenhouse plastic light transmissivity method. The estimation performance of the models was evaluated using the statistical indicators of the p-value of the slope, determination coefficient (R2), Nash–Sutcliffe model efficiency coefficient (NSE), root mean square error (RMSE), mean absolute error (MAE), relative error (RE), and Willmott Index (d). Compared with the other models, the Bristow and Campbell model showed a slightly higher performance in all whitening applications. Although the light transmissivity coefficient method performed slightly better than the temperature-based Rs estimation model, there was no statistical difference in the performances of the estimation models. Temperature-based estimation models offer a highly viable alternative for individuals who rely on the light transmittance approach to estimate Rs in greenhouses. This method can prove particularly useful in areas where measuring Rs outside the greenhouse is not possible or where partial time measurements cannot be taken owing to equipment malfunctions. All calibrated models can be used to estimate solar radiation using temperature data from various Mediterranean countries with similar climates and greenhouse cultivation.

Cloud condensation nuclei activation properties of Mediterranean pollen types considering organic chemical composition and surface tension effects

Wind-dispersed pollen grains emitted from vegetation are directly injected into the atmosphere being an important source of natural aerosols globally. These coarse particles of pollen can rupture into smaller particles, known as subpollen particles (SPPs), that may act as cloud condensation nuclei (CCN) and affect the climate. In this study, we characterize and investigate the ability of SPPs of 10 Mediterranean-climate pollen types to activate as CCN. A continuous flow CCN counter (CCNC) was used to measure the activation of size-selected (80, 100 and 200 nm dry mobility diameter) particles at different supersaturations (SS). Hygroscopicity parameter (κ) for each SPP type and size has been calculated using κ-Köhler theory. Organic chemical speciation and protein content has been determined to further characterize pollen solutions. Furthermore, the surface activity of SPPs has also been investigated by using pendant drop tensiometry. All studied SPP samples show critical supersaturation (SSCrit) values that are atmospherically relevant SS conditions. Hygroscopicity κ values are in the range characteristic of organic compounds (0.1–0.3). We found that organic speciation and protein content vary substantially among pollen types, with saccharides and fatty acids being the only organic compounds found in all pollen types. A clear relationship between SPP activation and its organic composition was not observed. This study also reveals that all SPPs investigated reduce the surface tension of water at high concentrations but at diluted concentrations (such as those of activation in the CCNC), the water surface tension value is a good approximation in Köhler theory. Overall, this analysis points out that pollen particles might be an important source of CCN in the atmosphere and should be considered in aerosol-cloud interactions processes.