Mediterranean Streams Research Articles

Challenges of restoring streams to a free-flowing state in semi-arid Mediterranean areas: Lotic systems in Cyprus from the perspective of the Water Framework Directive

Hydromorphological alterations are the most common pressures that occur in freshwater ecosystems, largely responsible for the overall decline of freshwater biodiversity. The EU 2030 Biodiversity Strategy, recognizing the crucial role of hydromorphological alterations, sets the target of 25 000 km of free flowing rivers in Europe by 2030. In this article, we discuss the hydromorphological restoration and mitigation measures in stream ecosystems implemented in Cyprus, the most arid country in the EU. We also assess their ability to contribute to the accomplishment of a free-flowing state, and we highlight the need for restoration guidelines for semi-arid areas in light of the climate crisis and the anticipated changes in water flow regimes. Our study showed that important progress has been made towards reconnecting streams in Cyprus, with the Water Framework Directive (WFD) being the main driving force behind this effort. However, even in semi-arid areas with severe water scarcity, emphasis should be placed on the demolition of dams and other water storage obstructions, as they are primarily responsible for the interruption of stream continuity. Thus, the development of a prioritization tool is required. Although we focus on the hydromorphological restorations in Mediterranean streams, we argue that the issues raised in this paper concern other geographical regions and river types with similar flow regimes and future challenges.

Dam removal effects on carbon processing in a mountainous Mediterranean stream

The global prevalence of obsolete or unsafe old dams necessitates the development of effective restoration approaches and expanded knowledge in this field. This study evaluates the effects of dam removal on carbon processing by measuring key ecosystem functions - organic matter decomposition, whole-reach metabolism, and gaseous carbon fluxes - in a mountainous Mediterranean stream. We compared these functions among three reaches: one where a dam was removed (restored), one with an intact dam (impacted), and one in natural conditions (reference). The measurements were conducted throughout the different seasons over the course of one year. Temperature-corrected organic matter decomposition rates and metabolic parameters in the restored reach showed intermediate values between those in the reference and impacted reaches. Additionally, dam removal resulted in carbon dioxide fluxes similar to those in the reference reach, whereas methane fluxes tended to be higher in the restored reach compared to the other reaches. Seasonal variation was high, and the observed effects were inconsistent across seasons for several functions. This inconsistency is likely due to uneven seasonal changes in the hydromorphological and physicochemical characteristics of the studied reaches. Our results indicate that, despite notable improvements, a longer timeframe is necessary for the restored reach to fully emulate the functional characteristics of the reference reach. While restoration by dam removal positively contributes to certain aspects of carbon processing, a more holistic approach, possibly encompassing broader hydromorphological and habitat enhancements, is needed to fully restore ecological processes in stream ecosystems. These insights are critical for informing future dam removal restoration projects, advocating the use of ecosystem function metrics as comprehensive indicators of ecological recovery and restoration success.

Persistent and lagged effects of fire on stream solutes linked to intermittent precipitation in arid lands

Increased occurrence, size, and intensity of fire result in significant but variable changes to hydrology and material retention in watersheds with concomitant effects on stream biogeochemistry. In arid regions, seasonal and episodic precipitation results in intermittency in flows connecting watersheds to recipient streams that can delay the effects of fire on stream chemistry. We investigated how the spatial extent of fire within watersheds interacts with variability in amount and timing of precipitation to influence stream chemistry of three forested, montane watersheds in a monsoonal climate and four coastal, chaparral watersheds in a Mediterranean climate. We applied state-space models to estimate effects of precipitation, fire, and their interaction on stream chemistry up to five years following fire using 15 + years of monthly observations. Precipitation alone diluted specific conductance and flushed nitrate and phosphate to Mediterranean streams. Fire had positive and negative effects on specific conductance in both climates, whereas ammonium and nitrate concentrations increased following fire in Mediterranean streams. Fire and precipitation had positive interactive effects on specific conductance in monsoonal streams and on ammonium in Mediterranean streams. In most cases, the effects of fire and its interaction with precipitation persisted or were lagged 2–5 years. These results suggest that precipitation influences the timing and intensity of the effects of fire on stream solute dynamics in aridland watersheds, but these responses vary by climate, solute, and watershed characteristics. Time series models were applied to data from long-term monitoring that included observations before and after fire, yielding estimated effects of fire on aridland stream chemistry. This statistical approach captured effects of local-scale temporal variation, including delayed responses to fire, and may be used to reduce uncertainty in predicted responses of water quality under changing fire and precipitation regimes of arid lands.

Seasonal Effects on Health Status and Parasitological Traits of an Invasive Minnow in Iberian Waters.

Biological invasions are of special conservation concern in the Iberian Peninsula and other regions with high levels of endemism. Environmental variability, such as the seasonal fluctuations of Mediterranean streams, is a key factor that affects the spread of aquatic species in novel habitats. Fish parasites have a great potential to reflect such changes in the habitat features of freshwater ecosystems. The aim of this study consisted of seasonally analysing the health status and parasitological traits of non-native fish in Iberian waters. In particular, a strongly invasive population of Languedoc minnow Phoxinus septimaniae (leuciscid species native to south-east France) was assessed in Tordera Stream (north-eastern Iberian Peninsula, Mediterranean conditions). Fish were sampled in April, July, and October 2023 by electrofishing. Health status (external/internal organs) was significantly better in autumn (HAI = 28.8) than spring (HAI = 35.6). Life-cycle complexity was higher in spring (LCI = 1.98), whereas parasite abundance and Shannon diversity were significantly lower in autumn (TA = 19.6 and H' = 2.15, respectively). In October (more 'benign' environmental conditions in Iberian streams), minnows could display elevated foraging activity, with fish increasing their health condition and level of parasite resistance/tolerance. Overall results showed a particular seasonal profile of health and parasite infra-communities that allow this minnow species to thrive under highly fluctuating habitat conditions. This information could help environmental managers to control non-native fish in Mediterranean streams.

Local environment and fragmentation by drought and damming shape different components of native and non-native fish beta diversity across pool refuges

Pool refuges are critical for maintaining stream fish diversity in increasingly intermittent streams. Yet, the patterns and drivers of beta diversity of native and non-native fish in pool refuges remain poorly known. Focusing on Mediterranean streams, we decomposed beta diversity of native and non-native fish into richness difference (RichDiff) and species replacement (Repl), and local (LCBD, LCBDRichDiff and LCBDRepl) and species (SCBD) contributions. We assessed the influence of environmental and spatial factors associated with drought and damming fragmentations on beta diversity components and LCBDs, and of local species richness and occupancy on LCBDs and SCBD, respectively. Overall, non-native species showed a more limited occupancy of pool refuges than native fish. RichDiff dominated beta diversity, though it was influenced by drought and damming fragmentations for native fish and local environment for non-native fish. Repl for native fish was slightly influenced by local environment, but for non-native fish was largely driven by drought and damming, albeit with a contribution of local environment as well. LCBD and LCBDRichDiff increased in pools in low order streams for native fish and at low elevations for non-native fish, and with high or low species richness. SCBD was higher for native species with intermediated pool occupancy, but for non-native species with low occupancy. Our results suggest that stream fragmentation may drive native species loss and non-native species replacement in pool refuges, and that environmental filtering may shape non-native species loss. Pools in lower order streams harbouring unique species-rich or species-poor assemblages should be prioritize for conservation and restoration, respectively, and pools at low elevation with unique non-native assemblages should deserve control efforts. We encourage the partitioning of beta diversity and individual analysis of native and non-native fish in intermittent streams, which may be key in stressing the importance of pool refuges in safeguarding native fish diversity.

Dual effects of drying and urban pollution on leaf litter decomposition and shredder consumption in permanent and intermittent streams

In the present study we investigated the combined effect of flow intermittence and wastewater treatment plant (WWTP) effluent on leaf litter decomposition in two Mediterranean streams with permanent and intermittent flow regimes. The litter decomposition experiment was performed using black poplar litter bags where we measured litter mass loss, fungal biomass, aquatic hyphomycete sporulation and species richness and C:N ratio. Afterwards, the remaining litter material from the different treatments was offered to the shredder Potamophylax latipennis in a microcosm laboratory experiment, and its growth and consumption rates were studied. We hypothesised that drying would decrease leaf litter decomposition in the permanent stream more than in the intermittent stream and that WWTP effluent would compensate for drying effects when both stressors interacted. The results indicated that drying has the greatest impact on the permanent stream, decreasing decomposition, aquatic hyphomycete species richness, and leaf litter quality. In the intermittent stream, WWTP effluent decreases aquatic hyphomycete species richness, but drying increases it, showing an opposing effect and a synergistic interaction. In the permanent stream drying provoked an increase in the shredder consumption rate. Thus, the expected more frequent intermittence in rivers under climate change may threaten the processing of organic matter.

Nitzschiatranstagensis Morales, Novais, Wetzel, Morais & Ector (Bacillariophyceae) - the second record in the Mediterranean Region.

The Mediterranean Region holds significant ecological importance, characterised by its unique climate, biodiversity and the crucial role it plays in global ecosystems. Mediterranean streams are naturally highly-stressed environments mainly due to fluctuations in water quantity. River flow generally varies from perennial to ephemeral and temporary rivers constitute significant water resources. Streams that flow through Balearic Islands are subjected to these conditions. The majority of these streams sustain water flow for 4-5 months annually, with exceptions noted for streams associated with springs, which typically maintain water throughout most of the year.Benthic diatoms are widely recognised as reliable bioindicators of water quality, used in many aquatic ecosystems. Analysing diatom communities and their biodiversity serves as a valuable tool to ensure the ecological and sustainable utilisation of water resources as well as the accurate development of guidelines for their conservation.The field of diatom taxonomy and distribution plays a crucial role in advancing our understanding of aquatic ecosystems and their biodiversity. Species of the genus Nitzschia are extensively found throughout the Mediterranean Region, including the Balearic Islands. However, they have rarely been investigated in temporary streams. This study presents the first record of Nitzschiatranstagensis Morales, Novais, Wetzel, Morais & Ector, outside the type locality and being the second record in Europe. In this study, the authors found this taxon in one temporary stream of Majorca Island, Torrent des Castellot in November 2005 (Balearic Islands). Nitzschiatranstagensis occurred at 2.6% abundance in this stream with oligotrophic waters (0.052 mg∙l-1 of nitrate), slightly alkaline pH values (7.8) and water conductivity levels of 626.5 µS cm-1. This species was recorded in the biofilm of the stones together with other taxa such as Achnanthidiumminutissimum (Kützing) Czarnecki (39.2%), Gomphonemarosenstockianum Lange-Bertalot & Reichardt (28.9%) and Halamphoraoligotraphenta (Lange-Bertalot) Levkov (20.4%). The Nitzschiatranstagensis frustules found in the examined material have similar dimensions and a higher fibulae count (8-11 in 10 μm vs. 6-10 in 10 μm) compared to the type material of Nitzschiatranstagensis. The habitat characteristics in which this species was found are described, together with LM micrographs of this taxon.

Development of a new Diatom-Based Index (BDIAR) for biomonitoring Mediterranean streams using data from Algerian Rivers

Development of a new Diatom-Based Index (BDIAR) for biomonitoring Mediterranean streams using data from Algerian Rivers

Plume dispersal of a hyper-eutrophic micro-estuary to the eastern mediterranean during base flow and flood events

Rivers are the primary source of dissolved and particulate materials entering the oceans. Recent studies conducted in Mediterranean streams showed a decrease in freshwater flow which indicates that this oligotrophic region, and probably other semi-arid oligotrophic regions, are likely to become more affected by small stream discharge and an increase in flood event intensity and frequency which indicates the increasing importance of studying flood conditions. However, little is known about the dispersal patterns of small streams and micro-estuaries and their influence on the coastal environment. We investigated the discharge from the Alexander Stream micro-estuary into the eastern Mediterranean as a case study for the fate of nutrients and pollutants discharged from micro-estuaries. Our objective is to characterize the Alexander Stream plume dispersal during base flow and flood events using salinity as a tracer for solute dispersal and by measuring the concentrations of suspended matter. The advection pattern during base flow was characterized by a current that clings to shore and mostly flows southward as a thin layer. This steady influx enriches the coastal water with high nutrient loads and carries pollutants toward Beit Yannai recreational beach, chronically exposing the shore and its users year-round. During floods, the plume disperses mostly northward along the coastline, and a considerable volume of suspended sediments settles near the estuary outfall, creating a hotspot of potentially contaminated sediment. At the peak discharge of the flood, the high concentrations of suspended sediments form hyperpycnal flows seaward near the seafloor and along the shelf. These results show the influence of small streams on the marine and coastal environments in semi-arid regions, which is predicted to expand to more temperate zones in the future.

Benthic sediment as stores and sources of bacteria and viruses in streams: A comparison of baseflow vs. stormflow longitudinal transport and residence times

The presence of bacteria and viruses in freshwater represents a global health risk. The substantial spatial and temporal variability of microbes leads to difficulties in quantifying the risks associated with their presence in freshwater. Fine particles, including bacteria and viruses are transported and accumulated into shallow streambed (i.e., benthic) sediment, delaying the downstream transmission during baseflow conditions but contributing to their resuspension and transport downstream during stormflow events. Direct measurements of pathogen accumulation in benthic sediments are rare. Until now, the dynamic role of benthic sediment as both a store and source of microbes, has not been quantified. In this study, we analyze microbial abundance in benthic sediment along a 1 km reach of an intermittent Mediterranean stream receiving inputs from the effluent of a wastewater treatment plant, a known point source of microbes in streams. We sampled benthic sediment during a summer drought when the wastewater effluent constituted 100 % of the stream flow, and thus, large accumulation and persistence of pathogens along the streambed was expected. We measured the abundance of total bacteria, Escherichia coli (as a fecal indicator), and presence of enteric rotavirus (RoV) and norovirus (NoV). The abundance of E. coli, based on qPCR detection, was high (4.99∙102 gc /cm2) along the first 100 m downstream of the wastewater effluent input and in general decreased with distance from the source, with presence of RoV and NoV along the study reach. A particle tracking model was applied, that uses stream water velocity as an input, and accounts for microbial exchange into, immobilization, degradation, and resuspension out of benthic sediment during baseflow and stormflow. Rates of exchange into benthic sediment were 3 orders of magnitude higher during stormflow, but residence times were proportionately lower, resulting in increased longitudinal connectivity from up to downstream during stormflow. Model simulations demonstrated mechanistically how the rates of exchange into and out of the benthic sediment resulted in benthic sediment to act as a store during baseflow and a source during stormflow.

Suspended Sediment Transport in Mediterranean Streams: Monitoring and Load Estimation

The suspended sediment (SS) load provides valuable insights into soil loss magnitude, requiring comprehensive monitoring of streamflow (Q) and SS concentrations (SSC) across various hydrological conditions. The primary aim of this paper was to quantify SS loads in two mountainous river basins: the Carapelle (506 km2) and the Celone (72 km2) located in Apulia (Southeast Italy) where different monitoring strategies were adopted (i.e., continuous and discrete). The specific objective was to develop sediment rating curves to address gaps in the SSC time series. An optical probe was used to continuously monitor the SSC during 2007 to 2011 in a river section of the Carapelle river, while Q was measured with the ultrasonic method. A comprehensive dataset comprising continuous Q measurements and discrete SSC measurements was systematically acquired for the Celone river over the period of 2010 to 2011. Distinct sediment rating curves were formulated for three specific subsets of data delineated by discernible hydrological conditions (i.e., high, normal, and low flow) and SSCs were computed for the missing daily records. The annual specific sediment load exhibited a range of 2.4 to 6.06 t ha−1 yr−1 for the Celone river, while the Carapelle river displayed a range of 0.9 to 7.45 t ha−1 yr−1. A significant majority of the SS load was transported during high-flow conditions, accounting for over 80% of the total load. In contrast, during low-flow conditions, the SS load constituted less than 1% of the total load. The findings of this study highlight the significance of the hydrological regime as a critical factor influencing sediment transport in mountainous Mediterranean rivers. Furthermore, it demonstrates that the duration of the sampling period, along with its specific characteristics, such as dry or wet conditions, can have a substantial impact on the accurate quantification of the sediment load.

Dissolved organic carbon bioreactivity and DOC:DIN stoichiometry control ammonium uptake in an intermittent Mediterranean stream

Abstract Heterotrophic organisms in streams use dissolved organic carbon (DOC) and dissolved inorganic nitrogen (DIN) from the water column to meet their growth and energy requirements. However, the role of DOC availability in driving DIN uptake in headwater streams is still poorly understood. In this study, we focus on how DOC:DIN stoichiometry and DOC bioreactivity control ammonium (NH4+) uptake and heterotrophic aerobic respiration, and how this influence varies among seasons in a forested Mediterranean headwater stream. We estimated in‐stream NH4+ uptake rates seasonally by conducting whole‐reach constant‐rate additions of NH4+ with and without amendments of either lignin (recalcitrant DOC) or acetate (labile DOC). During each addition, we characterised microbial community composition by molecular analyses, stream metabolism with the single‐station method, and heterotrophic aerobic respiration by adding a metabolic tracer (resazurin). The stream was heterotrophic (net ecosystem production <0) regardless of the season, with a microbial community mostly composed of heterotrophic bacteria. In‐stream NH4+ uptake rates were not related to either background NH4+ or DOC concentrations. Instead, these rates increased with increasing the molar ratio of NH4+ to nitrate (NO3−) (NH4+:NO3−) and DOC to DIN (DOC:DIN).Whole‐reach heterotrophic aerobic respiration rates showed the same relationship against stoichiometric ratios as NH4+ uptake rates. Furthermore, in‐stream NH4+ uptake rates were from 5% to >800% higher during the co‐additions of acetate than when adding NH4+ either alone or with lignin. Our results indicate that in‐stream NH4+ uptake was largely controlled by heterotrophic bacteria, and that the stoichiometric balance between organic resources and nutrients was key to explaining the variability of in‐stream NH4+ uptake and heterotrophic aerobic respiration. Moreover, the observed increase in NH4+ uptake during acetate additions suggests that heterotrophic activity was limited by labile DOC availability. Our study highlights that both DOC:DIN stoichiometry and DOC bioreactivity are relevant factors driving the seasonal pattern of in‐stream N processing in this forested Mediterranean headwater stream.

New data on freshwater lichens in Mediterranean streams

New chorological data on freshwater lichens in central Spain are presented. We have identified twenty-one saxicolous species resistant to periodic immersion at five localities of Montes de Toledo. Eighteen species are reported for the first time in Castilla-La Mancha territory. Remarkable extensions in the distribution range are found for most lichen species highlighting the importance of intermittent streams for lichen diversity in the Mediterranean Region

Alder stands promote N-cycling but not leaf litter mass loss in Mediterranean streams flowing through pine plantations

During the last century, the abandonment of traditional dryland farming and pastures in the Mediterranean basin promoted the development of ambitious afforestation programs causing a drastic transformation of the landscape. Afforestation programs were usually accomplished without considering the potential ecological impacts on the recipient ecosystems. Forest streams rely on terrestrial organic detritus, so their functioning and conservation status can be altered by changes in riparian forest biodiversity and composition. However, the influences of conifer plantations in particular on stream functioning are still unclear, possibly because the presence of multiple species in plantations or the presence of other species in the riparian zones of streams may act as buffers of such effects. Here, by means of a field experiment, we assessed whether the presence of black alder in the riparian zone and/or as leaf litter within the stream may mitigate the impacts exerted by pine plantations on stream ecosystem functioning. We found (i) that streams were functionally similar but differed in water N concentrations; (ii) no differences in litter mas loss between riparian types, but higher total litter mass loss of those mixtures containing alder leaf litter; and (iii) higher N losses (or lower N gains) for all litter types in streams without riparian alder. These results demonstrate that microbial decomposers can use either stream water N or litter N, and that detritivores can feed simultaneously on resources of contrasting quality to balance their diet. Our study underscores the effect of even low-density riparian cover of alder promoting microbial nutrient cycling by moderate increases of water N concentrations. We suggest thinning of pine plantations combined with planting of native deciduous species as alder to alleviate the effects of pine plantations on Mediterranean streams.

A Qualitative Assessment of River Plumes Coupling SWAT Model Simulations and a Beach Optical Monitoring System

The study of plumes occurring at the mouth of small rivers of temporal flow is a challenging task due to the lack of sedimentological and flow data of appropriate spatiotemporal scales. The present contribution examined the case of a typical un-gauged intermittent Mediterranean stream located in Northern Crete (Xiropotamos river). The SWAT (soil and water assessment tool) model was used to simulate and reproduce the hydrological behavior of the adjacent intermittent (Giofyros) river discharging at the same beach, the basin of which has the same geomorphological and hydrological characteristics. The output of the calibrated SWAT model was used to simulate daily flow data for the year 2014. The results were then considered together with the results of the RGB analysis of optical datasets of high spatio-temporal resolution for the same period, derived from a beach optical monitoring system (BOMS). The RGB analysis of the optical (TIMEX) imagery was shown to be a useful technique to identify and classify coastal plumes by using the spatio-temporal variability of pixel properties. The technique was also shown to be useful for the (qualitative) validation of the SWAT output and could be further improved by the collection of ‘ground truth’ data.

Regional environmental and climatic concerns on preserving native gene pools of a least concern species: Brown trout lineages in Mediterranean streams

The European brown trout, Salmo trutta, is a cold-adapted fish reported as a Least Concern species in the IUCN Red List. This species colonized new territories from southern refuges during the last glacial melting, but during the 20th century suffered from anthropic impacts on its habitats. The long-time survival of the species relies on the genetic diversity within and among populations. Brown trout is among the genetically most diverse vertebrate species; however, native populations in Mediterranean rivers have dramatically suffered of introgressive hybridization from extensive releases of evolutionary distant non-native Atlantic stocks. In addition, in Mediterranean rivers climate change will result in unsuitable conditions for the species during the 21st century. Using brown trout populations at the headstreams of a Pyrenean river as a model, this paper revised how hatchery releases have affected the native gene pools and how environmental and climatic variables controlled the amount of local introgression at intra-basin level. Introgressive hybridization was detected in all studied sites. Ten times larger divergence was observed among populations at tributaries than among populations along the main stem. A highly impacted population distributed in a long transect in the main stem suggested that hatchery fish move towards the main stem wherever released. From already highly impacted populations and despite the cessation of hatchery releases, warmer temperatures and lower precipitation expected from climate change will extend the introgressive hybridization along the basin, contributing to the extinction of the native gene pools. Based on available morphological distinction of native, hatchery and hybrid brown trout, we advocate the involvement of regional social groups (e.g. riverside dwellers, anglers, conservationists, hikers) in citizen science programs to detect the spread of non-native phenotypes along the rivers. These are cheap and fast methods to collaborate with fishery managers in the preservation and recovery of the regional native populations.

Machine learning models to predict nitrate concentration in a river basin

Aquifer-stream interactions affect the water quality in Mediterranean areas; therefore, the coupling of surface water and groundwater models is generally used to solve water-planning and pollution problems in river basins. However, their use is limited because model inputs and outputs are not spatially and temporally linked, and the data update and fitting are laborious tasks. Machine learning models have shown great potential in water quality simulation, as they can identify the statistical relationship between input and output data without the explicit requirement of knowing the physical processes. This allows the ecological, hydrological, and environmental variables that influence water quality to be analysed with a holistic approach. In this research, feature selection (FS) methods and algorithms of artificial intelligence—random forest (RF) and eXtreme Gradient Boosting (XGBoost) trees—are used to simulate nitrate concentration and determine the main drivers related to nitrate pollution in Mediterranean streams. The developed models included 19 inputs and sampling of nitrate concentration in 159 surface water quality-gauging stations as explanatory variables. The models were trained on 70 percent data, with 30 percent used to validate the predictions. Results showed that the combination of FS method with local knowledge about the dataset is the best option to improve the model’s performance, while RF and XGBoost simulate the nitrate concentration with high performance (r = 0.93 and r = 0.92, respectively). The final ranking, based on the relative importance of the variables in the RF and XGBoost models, showed that, regarding nitrogen and phosphorus concentration, the location explained 87 percent of the nitrate variability. RF and XGBoost predicted nitrate concentration in surface water with high accuracy without using conditions or parameters of entry and enabled the observation of different relationships between drivers. Thus, it is possible to identify and delimit zones with a spatial risk of pollution and approaches to implementing solutions.

Tectonics may affect closed watersheds used to monitor climate change and human activity effects

AbstractPlaya lakes developed in closed watersheds have been used as environmental impact indicators (global warming, water extractions, etc.), but they could be also sensitive to active tectonics. Our study area is located in the main watershed in the western Betics, the northern branch of the Gibraltar arc, which is within the fold‐and‐thrust belt. This area is characterized by an endorheic zone that includes some playa lakes floored by low‐permeable rocks. Our study shows the reduction or disappearance of playa‐lake flooded surfaces due to the reduction in their watershed area. This area reduction is favoured by stream catchments related to knickpoints, which seem to be generated by the quaternary activity of a dextral‐transpressive band within the Betics fold‐and‐thrust belt. χ map also shows the higher erosion power of Mediterranean streams, which will likely catch this endorheic area in tectonics quiescence.Statement of significancePlaya lakes developed in closed watersheds are commonly used to study environmental impacts such as global warming or water extractions. In this work, we analyse how these endorheic systems are sensitive to small water balance variations due to active tectonics. The endorheic area we chose to apply this new approach is located in the main Atlantic‐Mediterranean water divide of the western Betics (northern branch of the Gibraltar arc). The recent tectonics in this fold‐and‐thrust belt segment provoked the Atlantic‐Mediterranean gateway closure and the Messinian Salinity Crisis at 5.6 My. Our study of playa‐lake flooded surface and watershed modifications, together with structural and other geomorphic analyses such as the χ index, never before has it been made to detect active structures. Active tectonics likely influenced in the medium‐term evolution of the studied watersheds and this study is useful to predict both landscape and drainage network evolution in the future. In fact, the possible effect of active tectonic should be taken into account when using closed watersheds to monitor the impacts of climate change and/or local stressors such as water pumping activities.

Comparison of quantitative and semi-quantitative sampling methodologies for biomonitoring of Mediterranean streams using benthic macroinvertebrates: a case study from Greece

In Greece, the implementation of the Water Framework Directive for rivers is based mainly on benthic macroinvertebrates and uses a semi-quantitative method with a D-frame net, which is certified by the International Organization for Standardization. Before the official adoption of this method, a quantitative shovel sampler (“Cretan shovel”) was used in southern Greece (Crete), which has been implemented for almost three decades due to the specific river habitats found in Crete (e.g., seasonal flow, narrow riverbeds, and coarse substrates). In this study, we compared community metrics, diversity indices, feeding groups, locomotion types, and ecological quality derived from data collected using timed semi-quantitative kick samples and quantitative shovel samples collected from the same sites simultaneously. In total, 20 samples from the north and south of Greece were collected. The majority of community metrics, diversity indices, and traits were comparable between samplers. However, there were statistically significant differences in the relative abundance of Ephemeroptera, Plecoptera, and Trichoptera, passive filterers’ and the (semi) sessil groups, and Pielou’s index. Most differences in the ecological quality between the kick and shovel samples were observed in 50% of the sites in northern Greece because the shovel is less effective at capturing motile zoobenthos. The ecological quality assessment in Crete by the Cretan shovel is comparable with the D-frame net in 75% of the samples. Thus, the Cretan shovel could efficiently sample the Cretan streams, which are characterized by coarse, narrow, turbulent, and hydrologically fluctuating river habitats. Such comparisons could improve sampling effectiveness and make additional data available to assess ecological quality.

Applying Electrical Resistivity Tomography and Biological Methods to Assess the Hyporheic Zone Water Exchanges in Two Mediterranean Stream Reaches

The hyporheic zone (HZ) is a critical area of all river ecosystems. It is the area beneath the stream and adjacent to the stream, where the surface water and groundwater are mixed. The HZ extends both vertically and laterally depending on the sediment configuration, namely their porosity and permeability. This influences the hyporheic communities’ structural pattern and their active dispersal among distinct rivers compartments and alluvial aquifers. It is still difficult to assess the spatial extent of the HZ and the distribution of the mixing zones. This study applies time-lapse images obtained using electrical resistivity tomography (ERT) of 20 m wide and 5 m deep alluvial streams, with regards to the structural pattern of hyporheic communities represented by cyclopoids and ostracods, in order to assess the extent of the HZ in the riverbed and the parafluvial sediment configurations. The ERT images obtained at the hyporheic Site 1 are characterized by alluvial deposits dominated by coarse and very coarse sands with resistivity values ranging from ~20 to 80 Ohm.m, indicating a permeable zone up to ~0.5 m thick and extending laterally for ca. 5 m from the channel and associated with the hyporheic zone. The sediment configurations, texture, and structure indicate an active surface–hyporheic water exchange and low water retention into the sediments. This is also indicated by the hyporheic copepods and ostracods communities’ structure formed by a mixture of non-stygobites (five species) and stygobites (two species). A low-resistivity (<70 Ohm.m) permeable zone located 2.3 m below the streambed and unconnected with the river channel was also detected and associated with the associated alluvial aquifer. In contrast, the resistivity image at Site 2 dominated by coarse, medium, and very fine sands, shows a low-permeability zone in the upper ~0.5 m of the profile, with a resistivity value ranging from ~45 to 80 Ohm.m, indicating a reduced HZ extension in both vertical and lateral dimensions. Here the sediment configurations indicate that the water retention and interaction with the sediment is higher, reflected by more diverse hyporheic communities and with highly abundant stygobite species. The two examples show that non-invasive ERT images and biological assessments provide complementary and valuable information about the characterization of the sub-channel architecture and its potential hydraulic connection to the floodplain aquifer.