Mediterranean Locations Research Articles

Atlantic bonito (Sarda sarda) genomic analysis reveals population differentiation across Northeast Atlantic and mediterranean locations: Implications for fishery management

The Atlantic bonito (Sarda sarda, family Scombridae) is a pelagic species and one of the most exploited small tuna species. Despite its economic importance, biological information is scarce with no associated management directives. In this study, using a population genomic approach resulted in a lack of panmixia of two genetic pools with different effective population sizes (east central-tropical Atlantic and northeast Atlantic-Mediterranean) and an intermixing zone in Atlantic Morocco. The absence of genetic heterogeneity between the locations in Atlantic - Mediterranean transitional zone adds new evidence that challenges the Strait of Gibraltar as a phylogeographic barrier for marine pelagic species. These results are proposed to the ICCAT Commission to establish management areas for this species, although they are not consistent with the recently adopted pelagic ecoregions. Finally, a panel of highly informative SNPs was developed for efficient and low-cost population assignment and the analysis of unresolved population structures.

Mediterranean meteotsunamis of May 2021 and June 2022

Meteorological tsunamis (i.e., tsunami-like waves of atmospheric origin) are regularly observed in the Mediterranean Sea. During a single event, destructive flooding usually occurs in one location or limited area. However, in May 2021 and June 2022, strong meteotsunamis hit several Mediterranean locations up to 500 km apart. In the morning hours of the 24th of May 2021, a meteotsunami hit Bonifacio on the island of Corsica (western Mediterranean, France) and in the afternoon hours of the same day, another meteotsunami hit Široka Bay on the island of Ist (Adriatic Sea, Croatia), 500 km away. About 13 months later, on the 26th of June 2022, a meteotsunami hit Ciutadella on the island of Menorca (Spain) and two days later Bonifacio, 400 km away. Sea-level and atmospheric pressure data and satellite imagery, as well as synoptic conditions, associated with both events were analysed in detail. It has been confirmed that in the Mediterranean, meteotsunamis occur when meteotsunamigenic synoptic conditions prevail over the area, with a strong southwesterly jet stream embedded in dynamically or convectively unstable atmospheric layers standing out as the most important condition. The meteotsunamigenic potential of each of the three bays (Ciutadella, Bonifacio, Široka Bay) was investigated by considering: (1) the potential for Proudman resonance on the shelves offshore of the bays, (2) the orientation of the mouth of the bay and (3) the frequency of meteotsunamigenic synoptic conditions over the area. The strongest and most frequent meteotsunamis occur at locations where the shelf characteristics (width, depth, orientation), bay mouth orientation and distribution of general synoptic conditions, have characteristics that support the amplification of long-ocean waves and their propagation toward the bay mouth.

Cultivable mycoflora on bleached, decaying and healthy Posidonia oceanica leaves in a warm-edge Mediterranean location

Marine fungi are widely distributed in the ocean, playing an important role in the ecosystems, but only little information is available about their occurrence and activity. Seagrass bleaching is also a neglected phenomenon that seems to be linked to warm environments, even though the causes are still to be defined. In this study, the cultivable mycoflora associated to the leaf conditions (bleached, necrotic and live) and section (from the base to the tip) in the seagrass Posidonia oceanica was investigated in a Mediterranean warm-edge location (Cyprus). A total of 17 Ascomycota species/taxon were identified and results highlighted that mycoflora composition changed significantly in relation to both the leaf condition and section. A few known pathogens of terrestrial plants were detected only on bleached leaves, but it remains unknown whether they have any direct connections with P. oceanica bleaching phenomenon.

Exploitation shifted trophic ecology and habitat preferences of Mediterranean and Black Sea bluefin tuna over centuries

AbstractDuring recent decades, the health of ocean ecosystems and fish populations has been threatened by overexploitation, pollution and anthropogenic‐driven climate change. Due to a lack of long‐term ecological data, we have a poor grasp of the true impact on the diet and habitat use of fishes. This information is vital if we are to recover depleted fish populations and predict their future dynamics. Here, we trace the long‐term diet and habitat use of Atlantic bluefin tuna (BFT), Thunnus thynnus, a species that has had one of the longest and most intense exploitation histories, owing to its tremendous cultural and economic importance. Using carbon, nitrogen and sulphur stable isotope analyses of modern and ancient BFT including 98 archaeological and archival bones from 11 Mediterranean locations ca. 1st century to 1941 CE, we infer a shift to increased pelagic foraging around the 16th century in Mediterranean BFT. This likely reflects the early anthropogenic exploitation of inshore coastal ecosystems, as attested by historical literature sources. Further, we reveal that BFT which migrated to the Black Sea–and that disappeared during a period of intense exploitation and ecosystem changes in the 1980s–represented a unique component, isotopically distinct from BFT of NE Atlantic and Mediterranean locations. These data suggest that anthropogenic activities had the ability to alter the diet and habitat use of fishes in conditions prior to those of recent decades. Consequently, long‐term data provide novel perspectives on when marine ecosystem modification began and the responses of marine populations, with which to guide conservation policy.

Diffuse and Direct UV Index Experimental Values

In this work, we study the different components of the UV Index, direct, diffuse, and global, for seven years of UV erythemal measurements in a Mediterranean locality at Burjassot AtmoSpheric Station (BASS). The measurements have been taken with three YES-UVB-1 radiometers: one provided with a shadow disk on a Sun tracker; a radiometer to measure the global horizontal radiation; and a Sun-tracking radiometer to measure the direct radiation with an especially designed radiance collimator. It is known that diffuse UV irradiance represents at least 50% of total UV irradiance on a horizontal plane, which means being able to obtain a high UV Index even under shade. Our study aims to establish the relationship between the UV Index at noon and the maximum daily UV erythemal irradiance in the mid-latitude area, as well as the potential erythemal effects of exposure to solar UV radiation on different skin phototypes (skin phototypes I–IV) for both direct and diffuse components.

The effect of components capacity loss on the performance of a hybrid PV/wind/battery/hydrogen stand-alone energy system

A stand-alone hybrid renewable energy system (HRES) comprising a photovoltaic (PV) array and a small wind turbine for energy production, a lead-acid battery for short-term energy storage, and a hydrogen subsystem for long-term energy storage is analyzed in this study. The hydrogen subsystem comprises a PEM electrolyzer for hydrogen production, a hydrogen compressor and a hydrogen tank for hydrogen storage, and a PEM fuel cell for power generation. Twenty-year hourly input weather data were acquired from the national meteorological service for the Mediterranean location of Split, Croatia. Two different end-user load profiles are considered, namely a year-around constant load profile and the stochastically variable load profile. Furthermore, a comparative analysis is performed for two different energy management strategies – one based on energy balance within the system, and the other based on double hysteresis loop control. The HRES performance is simulated using MATLAB. The dynamics of capacity loss of the batteries, PEM electrolyzer, and PEM fuel cell is a function of how the system operates, but at the same time, this dynamics of capacity loss affects the overall system performance. The double hysteresis control strategy results in a significantly longer battery lifetime for both load profiles considered, while the PV and battery loss of capacity causes more frequent electrolyzer and fuel cell operation for all considered cases.

A critical analysis on the use of different weather datasets to assess moisture-related risks in building components for a Mediterranean location

In the field of numerical hygrothermal simulations, the literature suggests that multiyear weather datasets should be used to predict long-term moisture-related risks in building components. However, to improve the computational time-efficiency of hygrothermal analyses, single year simulations are preferable, although their reliability might be questionable. This study investigates different commonly used methodologies to define single year datasets for hygrothermal simulations, in order to identify advantages and limitations in terms of time-efficiency, data availability, and reliability of the results. To this aim, an initial literature analysis has reviewed many previous studies to identify the most used types of weather dataset, namely Multiyear (MY), Moisture Reference Years (MRY), and Typical Years (TY). Then, numerical simulations of different wall assemblies located in a Mediterranean city show the effect of the above datasets in the evaluation of mold growth risk and increased heat losses due to moisture accumulation, without disregarding the importance of computational time. The paper demonstrates that, in a warm European climate, an MRY does not necessarily ensure more conservative results than a TY, compared with MY-based simulations. Non-negligible discrepancies in the assessment of the mold growth risk emerge in case of interior wall insulation, while walls with exterior insulation are far less affected by the choice of the weather dataset. Moreover, a TY allows estimating the increased heat losses with an error less than 0.5%. One further result is that an MRY does not ensure low time effort compared to MY datasets, due to the pre-processing required to collect and elaborate the weather data for a long-term period.

Genetic Variability and Connectivity in the Western Mediterranean Populations of the Bathyal Crab Geryon longipes

Geryon longipes is a crab species that inhabits the muddy bottoms of the middle and lower slopes, as well as bathyal bottoms ranging from 400 to 2000 m in depth. To assess its molecular diversity, a fragment of 572 bp of the COI (Cytochrome Oxidase subunit I) mitochondrial gene was sequenced in eight Western Mediterranean locations. Within the studied area, two oceanographic fronts are present (Almeria-Oran Front and Ibiza Channel). From the 124 sequences obtained, only 7 distinct haplotypes were identified. The population distribution indicated three well-differentiated regions: the Alboran Sea, the Gulf of Vera and the Levantine/Catalan coasts. The molecular diversity was compared with that obtained in the same year for the same gene in Liocarcinus depurator, a crab species that is captured on the continental shelf and upper slope (40 to 500 m). The estimates of molecular diversity parameters for the COI gene fragment were rather similar between both species, but the number of haplotypes was higher for L. depurator. Finally, the obtained COI sequences of G. longipes were compared to those from other populations of the species distribution range, recovered from the DNA repository. Only one additional, different haplotype was reported (Sicily), whereas all the rest were common with those described in our study. Therefore, the COI gene fragment would indicate that all the sequences analysed in the Mediterranean and NE Atlantic belong to the same species, G. longipes.

Comparing otolith shape descriptors for population structure inferences in a small pelagic fish, the European sardine Sardina pilchardus (Walbaum, 1792).

Otolith shape analysis has been one of the most used approaches to study population structure in the past decades. Currently, two sets of shape descriptors are used to perform otolith shape analysis, namely, Elliptic Fourier descriptor (EFd), which focuses on the overall otolith shape differences, and Discrete Wavelet descriptor (DWd), which is sensible to local differences along the otolith contour. Here, the authors conducted a comparative analysis of the performance of both the descriptors in reconstructing the population structure and connectivity patterns in a small pelagic fish species with a wide geographical distribution and fast growth rate, the European sardine Sardina pilchardus (Walbaum, 1792), for the first time. A combination of each otolith shape descriptor and shape indices was explored using multivariate statistical methods. The two otolith shape descriptors showed similar, although limited, overall classification success associated with the population dynamic characteristics of the species. Both descriptors point to migration among adjacent areas, such as northern Atlantic locations, eastern Mediterranean and even across well-defined physical obstacles, such as the Strait of Gibraltar, among Atlantic and western Mediterranean locations. Both descriptors supported the division of the populations of Mediterranean waters into three main groups but slightly differed in the group limits of the Atlantic waters. A comparison of the present results with those from previous otolith shape analysis studies using EFd on a decadal time scale revealed differences in the population structure and connectivity patterns compared to the earlier period. These differences not only may be attributed to changes in environmental variables leading to changes in population dynamics but can also be the result of the sardine biomass sharp decrease that occurred in the past decade.

Alternaria alternata causing inner black rot of lemon (Citrus limon) fruits in Turkey: Genetic diversity and characterisation

The market sales of symptomless lemons in 2020 revealed a persistent and severe internal black rot with browning of the membrane wall and juice sac. To investigate the causal agent, externally symptom-free lemons from Mediterranean locations were sampled from supermarkets, local markets, and bazaars in three southeastern Turkish provinces. Internal black rot symptoms were observed in 13.69% of the 1300 externally symptomless fruits, and Alternaria was isolated from 96.63% of those exhibiting these symptoms. Morphological analysis confirmed the identity of Alternaria alternata in 26 representative isolates collected from various markets and bazaars. To examine genetic diversity or polymorphisms, start codon targeted (SCoT) markers were used. Species identification was based on the sequences of translation elongation factor 1-alpha (TEF 1-α), RNA polymerase second largest subunit (RPB2), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH). SCoT 29 exhibited the highest amount of polymorphic amplicons, with 18 polymorphic bands scored when compared to the other six scoreable markers. The 26 representative isolates from various markets and bazaars were divided into four clades by SCoT 29. However, multilocus sequence analyses confirmed the identification of a single species, A. alternata. In terms of virulence and the impact of temperature on growth, representative isolates from four SCoT clusters were evaluated. Data suggests that SCoT markers can be used to evaluate virulence-based polymorphism in A. alternata; nonetheless, all clades showed comparable temperature responses, with 25 °C being the optimum. There was also no association between individual morphotypes and SCoT clade membership. Due to the lack of obvious signs on lemon fruits, producers, merchants, and consumers are unable to identify black rot, and the widespread presence of A. alternata in lemons may pose a significant risk that must be avoided at all costs.

Climatic Drivers of Silicon Accumulation in a Model Grass Operate in Low- but Not High-Silicon Soils

Grasses are hyper-accumulators of silicon (Si), which is known to alleviate diverse environmental stresses, prompting speculation that Si accumulation evolved in response to unfavourable climatic conditions, including seasonally arid environments. We conducted a common garden experiment using 57 accessions of the model grass Brachypodium distachyon, sourced from different Mediterranean locations, to test relationships between Si accumulation and 19 bioclimatic variables. Plants were grown in soil with either low or high (Si supplemented) levels of bioavailable Si. Si accumulation was negatively correlated with temperature variables (annual mean diurnal temperature range, temperature seasonality, annual temperature range) and precipitation seasonality. Si accumulation was positively correlated with precipitation variables (annual precipitation, precipitation of the driest month and quarter, and precipitation of the warmest quarter). These relationships, however, were only observed in low-Si soils and not in Si-supplemented soils. Our hypothesis that accessions of B. distachyon from seasonally arid conditions have higher Si accumulation was not supported. On the contrary, higher temperatures and lower precipitation regimes were associated with lower Si accumulation. These relationships were decoupled in high-Si soils. These exploratory results suggest that geographical origin and prevailing climatic conditions may play a role in predicting patterns of Si accumulation in grasses.

Mediterranean coastal wave-climate long-term trend in climate change scenarios and effects on the optimal sizing of OWC wave energy converters

This work assesses the effect of long-term changes in the wave climate on the optimal sizing and performances of oscillating water column wave energy converters along the coastline of the Mediterranean basin. Trends are evaluated based on the projections of future wave climate to 2100 delivered by the Climate Data Store of the Copernicus Climate Change Service under representative atmospheric emission scenarios. The capture width of the device under different wave conditions is computed using an empirical model capable of predicting the device performance with acceptable accuracy and limited computational time. The optimal geometry of the device varies significantly across the different geographical locations, with maximum values of the chamber size along the north African coasts and western Sardinia and Corse, and minimum values in the Adriatic and Aegean Seas. The effect of long-term change in the wave energy resource results in moderate modifications of the optimal geometry, with relative variations between present and future scenarios up to 10% of the size. The annual energy production of the device in the future scenario would significantly increase in most geographical locations, potentially contributing to reducing the Levelized Cost of Energy and favouring the economic competitiveness of this technology. • Effects of long-term changes in the wave climate on the performance of OWCs are studied. • Projections of future wave climate under IPCC emission scenarios are considered. • Trends in the wave power are significant in some Mediterranean locations. • The optimal device geometry in the present and future scenarios varies up to 10%. • The annual energy production in the future scenario could increases in most locations.

Assessment of elemental composition in commercial fish of the Bay of Cádiz, Southern Iberian Peninsula

The assessment of trace metal content in our fish diet is important due to the adverse effect on human health. Despite the increasing interest about the fish quality, little information is available for Southern Spain, a region characterized by high seafood intake. Nine species from the Bay of Cádiz with high commercial value were selected. Similar values were measured in the nine studied species for most of the elements, except for the macroelements Ca and S, and the microelements Fe, Mn and As, which showed significant differences among species. Metal Pollution Index (MPI) did not differ among species, and it was similar to those obtained for other Atlantic and Mediterranean locations. The values measured for the nine species were below the health limits provided by World, European and Spanish legislations, indicating that, in general terms, consumption of these species is safe in the study area.

A Study of Arca noae (Linnaeus, 1758) in Elounda Bay, Crete, Eastern Mediterranean

There is growing interest about marine bivalve aquaculture globally, not only for the market value of the goods produced, but also for the socio-economic and environmental services that this activity can provide. Arca noae is an endemic Mediterranean bivalve of commercial value, whose previously undescribed population in Elounda Bay we studied in terms of its structure and reproduction, while constructing a timeseries of the basic environmental parameters of the bay, thus, gaining fundamental knowledge for the potential future exploitation of the species in the area. We found a variable spatial distribution of arks in the study area, with local high peaks in the population density, consisting of smaller size individuals, in comparison to other areas. Because of protandry of the species, human pressure on this population could have a strong negative effect, by targeting the limited numbers of large females in the study area. The reproduction pattern was similar to the reports from other Mediterranean locations. The abiotic conditions in Elounda Bay differed from those in the adjacent coastal zone, confirming that the Bay is a unique semi-enclosed marine area in the island of Crete.

Concrete Blocks Reinforced with Arundo donax Natural Fibers with Different Aspect Ratios for Application in Bioarchitecture

In recent decades, the construction industry has advanced in its use of natural green resources, such as vegetable fibers (e.g., flax, hemp, jute, etc.) added in concrete mixtures, to create building materials that are both economically and environmentally sustainable. The pricing, low energy cost, and environmental sustainability of these natural fibers are driving this interest. The quantity of fibers and the ratio of fiber length to its transverse diameter (aspect ratio) are critical characteristics that have a decisive impact on concrete’s mechanical qualities. The influence of the aspect ratio of Arundo donax fibers on the tensile characteristics of concrete blocks was specifically investigated in this study. These fibers were collected from the outer section of the stem of this plant, which grows commonly in Mediterranean locations, but that is also found all over the world. Experiments were carried out on cylindrical concrete blocks with a constant amount of fiber (1 percent by weight) and different aspect ratios: 30, 50, and 70 (mm/mm) respectively, to assess their tensile strength, even when compared with concrete blocks without any fiber addition. Tensile tests on Arundo donax fibers were also conducted, with the aim to contribute to the analysis of their interaction with cementitious matrices, and to assess differences between the various compositions. The results showed a direct impact of the aspect ratio on the final tensile strength of concrete blocks, with higher aspect ratios producing superior tensile properties.

Surface Characteristics Together With Environmental Conditions Shape Marine Biofilm Dynamics in Coastal NW Mediterranean Locations

Microbial colonization of artificial substrates in coastal areas, which concerns hull ships, sensors as well as plastic debris, is of huge significance to attain a rational environmental management. Some surface and environmental drivers of biofilm development have previously been described but their relative impact on the formation of biofilms remains unknown while crucial. Especially, there is no evidence of the relative importance of physical surface properties (wettability, roughness, smoothness) compared to seawater characteristics in driving biofilm abundance and diversity. In addition, few studies have considered the temporal evolution of this complex form of colonization, which often prevent to globally understand the process. Using experimental facilities in two Mediterrranean locations, a multidisciplinary approach including surface characterizations as well as seawaterquality analyses, flow cytometry and 16S rDNA metabarcoding, allowed for the identification of the main drivers of colonization for two antifouling (AF) coatings. One AF coating released copper (SPC1) while the other limit colonization thanks to physical properties, namely a low surface energy, roughness and smoothness (FRC1). Results were obtained over 75 days and compared to a control surface (PVC). Biofilm development was observed on all surfaces, with increasing density from AF coatings to PVC. Pionneer bacteria were dissimilar within all three surface types, however, communities observed on FRC1 converged toward PVC ones overtime, whereas SPC1 communities remained highly specific. A remarkably low and unique diversity was found on SPC1 during the experiment as Alteromonas accounted for more than 90% of the community colonizing this substrate until 12 days, and remained one of the co-dominant taxa of mature biofilms. Moreover, clear differences were found between geographical locations. Low nutrients and higher hydrodymanics in Banyuls bay resulted in less dense biofilms overall compared to Toulon, but also in a the slower dynamic of biofilm formation. This is illustrated by the persistence of pioneer Alteromonas but also Hyphomonadacae after 75 days on SPC1. We concluded that, even if local environmental conditions influenced the composition of biofilm communities, particular physical features may control the biofilm density but not the diversity, while copper releasing coating controlled both. In addition, it is evident from these results that sequential biofilm dynamics should carefully be considered as initial processes of formation differed from the long-term ones.

Phenolic variability in fruit from the ‘Arbequina’ olive cultivar under Mediterranean and Subtropical climatic conditions

In the present work, we compared the phenol content and composition of fruit from the ‘Arbequina’ cultivar in four Mediterranean (in Andalucía, Southern Iberian Peninsula) and two Sub-Tropical (Canary Islands) locations throughout the harvest period. Two Mediterranean and two Sub-Tropical locations were maintained with drip irrigation, while the remaining two Mediterranean locations were in dry farming. Water availability and harvest date seemed to play more important roles than air temperature on the phenolic content and most of the studied components. The variability associated with location was a result of the high values observed in the two Mediterranean locations in dry farming, with respect to the other four maintained with drip irrigation. Few differences were found among the four drip-irrigated locations, despite the fact that two were Mediterranean and the other two Sub-Tropical. In addition, a sharp decrease was observed during the harvest period for phenolic content and most of the phenolic compounds.

Optimization of the thermal storage system in a solar-driven refrigeration system equipped with an adjustable jet-ejector

The present paper shows a numerical research about the influence of different thermal storage capacities and thermal power consumption strategies in a solar-driven air-conditioning system operating with refrigerant R1234yf. The computational model is fed with hourly climatic data (solar irradiance and ambient temperature) of the typical meteorological year of a Mediterranean location. A special focus is put on the dynamic response of the refrigeration system, the solar collector, and the sensible heat storage tank. Since the refrigeration needs are nearly synchronized with the sunny hours, small tank volumes are the most convenient architecture to achieve a rapid heating-up after tank discharges. For a parabolic trough collector span of 7.1 m, 13.3 kW of thermal power consumption represents a reasonable trade-off between the main performance indicators, mainly, the refrigeration capacity, COPth (thermal coefficient of performance) and the system's capability to operate properly ensuring an adequate thermal level in the heat reservoir.

Distribution of the seagrass Halophila stipulacea: A big jump to the northwestern Mediterranean Sea

The Red Sea and Indian Ocean seagrass Halophila stipulacea entered the Mediterranean in the late 19th century via the Suez Canal. Here, we report on the discovery of a population of H. stipulacea covering 16.5 ha off the harbour of Cannes, French Riviera, France. This represents the northernmost locality of the species and a jump of 350 km to the north from its closest western Mediterranean locality (Razza di Juncu in Sardinia). At Cannes, it was found dwelling between 11 and 17 m depth on a dead matte of Posidonia oceanica. The species has most probably been introduced through mega-yacht or cruise ship anchoring since the Bay of Cannes is among the most highly-frequented places for luxury yachting and tourism. The lack of effective regulation to prevent the introduction of non-indigenous species in France and Europe is highlighted.

Revisiting future extreme precipitation trends in the Mediterranean

Global warming is anticipated to intensify the hydrological cycle. However, this is neither expected to be globally uniform nor is the relationship between temperature increase and rainfall intensities expected to be linear. The objective of this study is to assess changes in annual rainfall extremes, total annual precipitation, and their relationship in the larger Mediterranean region. We use an up-to-date ensemble of 33 regional climate simulations from the EURO-CORDEX initiative at 0.11° resolution. We analyse the significance of trends for 1951–2000 and 2001–2100 under a ‘business-as-usual’ pathway (RCP8.5). Our future projections indicate a strong north/south Mediterranean gradient, with significant, decreasing trends in the magnitude of daily precipitation extremes in the south and the Maghreb region (up to −10 mm/decade) and less profound, increasing trends in the north. Despite the contrasting future trends, the 50-year daily precipitation extremes are projected to strongly increase (up to 100%) throughout the region. The 100-year extremes, derived with traditional extreme value approaches from the 1951–2000 simulations, underestimate the magnitude of these extreme events in the 2001–2100 projections by 30% for the drier areas of the Mediterranean (200–500 mm average annual rainfall) and by up to 20–30% for the wetter parts of the region. These 100-year extremes can occur at any time in any Mediterranean location. The contribution of the wettest day per year to the annual total precipitation is expected to increase (5–30%) throughout the region. The projected increase in extremes and the strong reductions in mean annual precipitation in the drier, southern and eastern Mediterranean will amplify the challenges for water resource management.