Field Of Medicine Research Articles

Inhibitory Effect of Oxibiol on the Process of Protein Modification by Water-Soluble Products of Photo-Oxidative Destruction of Bisretinoid A2E

It has previously been shown that heteroaromatic antioxidant oxibiol (N-acetylcysteinate 6-hydroxy-2-aminobenzothiazole) inhibits the process of fructosylation of serum albumin. The aim of this study is to elucidate the inhibitory effect of oxibiol on modification of proteins by photodegradation products of bisretinoid A2E, the main fluorophore of lipofuscin granules in retinal pigment epithelial cells of the human eye. It was shown that unlike a water-soluble fraction fraction from non-irradiated A2E-liposomes, a water-soluble fraction formed after irradiation of A2E-liposomes with visible light significantly modified albumin in a day after incubation at 37°C. Oxibiol in millimolar concentrations effectively inhibited this process. The inhibitory effect of oxybiol could be attributed to its antioxidant activity and the ability to compete with reactive aldehydes formed during the photooxidative degradation of A2E-cardiolipin liposomes. The acute toxicity of oxibiol in mice after intraperitoneal injection was studied, the values of LD 10 and LD 50 were determined. The results obtained demonstrate that oxibiol can be used in pharmacology for preventing and curing diseases associated with the development of oxidative stress in various fields of medicine, primarily in ophthalmology.

Transforming Healthcare in Low-Resource Settings With Artificial Intelligence: Recent Developments and Outcomes.

Artificial intelligence now encompasses technologies like machine learning, natural language processing, and robotics, allowing machines to undertake complex tasks traditionally done by humans. AI's application in healthcare has led to advancements in diagnostic tools, predictive analytics, and surgical precision. This comprehensive review aims to explore the transformative impact of AI across diverse healthcare domains, highlighting its applications, advancements, challenges, and contributions to enhancing patient care. A comprehensive literature search was conducted across multiple databases, covering publications from 2014 to 2024. Keywords related to AI applications in healthcare were used to gather data, focusing on studies exploring AI's role in medical specialties. AI has demonstrated substantial benefits across various fields of medicine. In cardiology, it aids in automated image interpretation, risk prediction, and the management of cardiovascular diseases. In oncology, AI enhances cancer detection, treatment planning, and personalized drug selection. Radiology benefits from improved image analysis and diagnostic accuracy, while critical care sees advancements in patient triage and resource optimization. AI's integration into pediatrics, surgery, public health, neurology, pathology, and mental health has similarly shown significant improvements in diagnostic precision, personalized treatment, and overall patient care. The implementation of AI in low-resource settings has been particularly impactful, enhancing access to advanced diagnostic tools and treatments. AI is rapidly changing the healthcare industry by greatly increasing the accuracy of diagnoses, streamlining treatment plans, and improving patient outcomes across a variety of medical specializations. This review underscores AI's transformative potential, from early disease detection to personalized treatment plans, and its ability to augment healthcare delivery, particularly in resource-limited settings.

3D printing in surgery: relevance of technology maturity assessment in bioprinting research studies

Biological 3D printing (bioprinting) is an extension of what is defined as additive manufacturing in the American Society for Testing and Materials (ASTM) and International Organization for Standardization (ISO) standards and is based on the automated printing of living cells and biomaterials. Researchers and experts in the field of biomaterial science, tissue engineering and regenerative medicine (TE&RM) are constantly pointing to the potential of biological 3D printing and scientific articles regularly announce the imminent clinical application. We argue in this article that these announcements are often premature and counterproductive as they focus heavily on technological progress but regularly ignore the critical stages that need to be completed in order to successfully translate atechnology into the healthcare market. The technology readiness level (TRL) scale is apotentially useful tool for measuring the relative maturity of atechnology in terms of overcoming aseries of critical milestones. We propose an adaptation of the TRL scale and use it to discuss the current state of research on biological 3D printing. Finally, we provide specific recommendations for optimizing future research projects to pave the way for clinical applications of biological 3D printing and thus achieve adirect positive impact on surgical patient care.

Urinary incontinence in elite female powerlifters aged 20-30: correlating musculoskeletal exam data with incontinence severity index and survey data.

Urinary incontinence (UI) in the powerlifting community has been a hot topic due to its noticeability during competition and the burden it places on female athletes who experience it. UI is even experienced in those we least expect: young, high-performing, females with no history of pregnancy. Current studies have utilized primarily survey methodology, thus there is a lack of clinical information on this topic. Furthermore, the top athletes are underrepresentedbecause previous surveys were open to anyone reporting themselves as a powerlifter, regardless of competition level. The objectives of this study were to determine whether UI in elite female powerlifters is correlated with any musculoskeletal diagnoses and to further evaluate potentially contributing factors for UI within this group. Subjects underwent an osteopathic structural examination and then completed two surveys: the Incontinence Severity Index (ISI/Sandvik Test for Urinary Incontinence) and the Study Questionnaire. In total, there were 31 female participants, all of whom were in the top 2 % of powerlifters in the world between the ages of 20 and 30 years old (as of May 21, 2023) and reside in the United States. The survey results displayed a moderately positive relationship between age (rho=0.449, where rho refers to Spearman's rho), history of pelvic floor examination (rho=0.413), and self-care practice with ISI (rho=0.340). Other survey information such as weight class, height, best total in competition, number of years training, average duration of training day, confidence in ability to contract pelvic floor muscles, history of abdominal or pelvic surgery, history of urinary tract infection (UTI), and sexual activity status all had rho values less than 0.300. With atlantoaxial (AA) rotation to the right, there was an increase in ISI score (p=0.009). Similarly, with AA rotation to the left, there was a decrease in ISI score (p=0.030). All patients with severe ISI had nonphysiologic sacral dysfunctions (p=0.051). Severe ISI-scored participants were more likely to yield a dysfunctional clavicle upon screening (p=0.027). There was a strong correlation between increasing severity of UI and findings of a restricted right clavicle and/or AA rotation to the right (p=0.010). In addition, there were only two individuals with both a restricted right clavicle and AA rotation to the right, and these two individuals both scored severe on the ISI (p=0.012). A pelvic diaphragm dysfunction was present in 74.2 % of the participants. Extension diagnoses of the lumbar spine were found in those with mild UI over those with severe UI (p=0.012). Most other diagnoses were largely unremarkable due to a wide distribution across all ISI scores. Our study revealed a relationship between UI and somatic dysfunctions in this population. These findings may help providers, especially in the fields of primary care, sports medicine, and urogynecology, to expand treatment options for UI in this group in the future. Survey results yielded a positive relationship of moderate strength between age, history of pelvic floor exination, and engaging in leakage prevention practices with ISI. No survey category was found to have a relationship of high strength with ISI. These findings contribute to our knowledge on factors that do (or do not) contribute to incontinence severity.

Professional development and job satisfaction in pain medicine

Pain medicine is an interdisciplinary and interprofessional field of specialisation. Due to concerns about new recruits and an aging workforce, especially among physicians, it is important to better understand professional and career pathways in pain medicine. The aim of this study was to record the occupational routes of people working in an institution specialised in pain medicine/pain management as well as their motivation and job satisfaction. Astandardised online questionnaire was used to survey members via cooperating scientific societies and organisations. Data from 398 physicians, 78psychologists, 62physiotherapists, three occupational therapists and 23nursing professionals were included in the analysis. The age distribution skewed towards higher age groups, with the majority of respondents aged between 51and 60years. The respondents usually first came into clinical contact with pain medicine after many years of work. In the case of physicians in particular, there was adelay of almost adecade after their licence to practise. With regard to motivational factors, intrinsic aspects were rated higher than monetary ones. Work-life balance issues tended to be in the middle of the ranking. Regarding job satisfaction in pain medicine, the overall satisfaction of the respondents was high, with the lowest satisfaction ratings being given in the categories "opportunities for further career development", "additional income options" and "recognition by superiors". Asignificant proportion of respondents (1/3) stated that career changes were forthcoming-changes outside apain medicine setting or in particular retirement. The results emphasise the critical perspectives regarding the future provision of care for people with chronic pain. The results provide, for the first time, an insight into the career routes of staff in the field of pain medicine and their motivations and job satisfaction, which should be considered in the discussion about ensuring personnel resources in the future.

Feature Papers in Bone Biomaterials

Bone biomaterials have garnered significant attention in the field of regenerative medicine due to their application in a wide range of clinical scenarios [...]

Plant Species with Antimicrobial Effects and the Importance of a Standardization of Protocols for Antimicrobial Agents

In modern times, microbial resistance is a global threat to health and development. The misuse and inappropriate use of antimicrobials is the main cause of developing drugresistant pathogens. It requires multiple areas in direction to attain sustainable advance goals. As a result of microbial resistance, the necessity for costly medications and expenses are obstructed worldwide. Due to rising attention in the research of new antimicrobial medicaments from a variety of natural or synthetic sources to fight microbial resistance. Thus, natural antimicrobial agents have been used to a great extent nowadays because plant-derived antimicrobial agents are considered to be safer alternatives for health as compared to those synthetic antimicrobial agents. Overall, the active ingredients, water, essential oils, and ethanolic extracts from selected plants and the mixture of a variety of these natural extracts have been used for centuries, because they possess antimicrobial activity which inhibits the growth of microbes. Natural plants as an antimicrobial agent, like extracts of Curcuma longa L., Piper nigrum L., Vachellia leucophloea, Eclipta prostrata, Ocimum sanctum L., Terminalia arjuna, Manihot esculenta Crantz, Lawsonia inermis L., Zingiber officinale Roscoe, Camellia sinensis (L.) Kuntze, Coriandrum sativum L., Carica papaya L., Cinnamomum tamala, and many others have been preferred and used for ages because they are easily available worldwide. They are usually of low cost and have little or no side effects. Several antimicrobial screening approaches like the disk-diffusion method, well diffusion method, micro broth dilution assay, sterile disk method, and agar diffusion method are generally cast off for measurement of reproducibility and standardization of these antimicrobial agents. This review article is a comprehensive description of natural plants like Coriandrum sativum L., Carica papaya L., Cinnamomum tamala, etc., containing those extracts used as antimicrobial agents listed, and numerous in vitro antimicrobial susceptibility testing methods are reported. These identified plant species and antimicrobial screening techniques hold the potential for formulating these plants into antimicrobial drugs, warranting further study and exploration in the field of medicine.

Performance evaluation of the mammography ai services

Backgraund: Digital mammography is a key tool for diagnosing breast cancer (BC), which can reduce mortality by 20-40% due to early detection of pathology. To date, there have been developed many AI services that allow you to automate the process of evaluating such studies. Aims: The purpose of this work is to compare the results of different types and versions of AI services in relation to physician assessments in the analysis of mammography studies. Materials and methods: We have compared several binary scales for assessing mammography examinations, as well as several types and versions of AI services using accuracy metrics, the Matthews coefficient, and the maximum Youden index. Results: As a result of a comparative analysis, it was shown that the number of detected pathologies and the accuracy of assessing AI services depends on the binary scale for evaluating of the digital mammography. In addition, the maximum Youden index allows us to estimate the statistically significant difference between services and scales. According to this metric, AI service 1 and its version 3 have the best performance, which is consistent with most accuracy metrics. Conclusions: These results can be used in any field of medicine during the process of choosing AI services for clinical deployment.

Narrow-Pulse-Width, Straight-Type-Cavity, All-Solid-State Laser at 228.5 nm

Deep-ultraviolet (DUV) lasers operating at a wavelength of 228 nm offer distinct advantages in Raman spectroscopy and analysis, demonstrating significant potential in the field of surgical medicine. This paper details the development of a high-repetition-rate, narrow-pulse-width, short-cavity laser system functioning at 228.5 nm, which is based on Barium Borate (BBO) electro-optic Q-switching. The system utilizes a double-concave resonator structure and a pressure-applied electro-optic Q-switching technique, incorporating Lithium Borate (LBO) and BBO as frequency-doubling crystals. A low-concentration Nd:YVO4 crystal, measuring 4 mm × 4 mm × 5 mm, serves as the gain medium, with a high-reflectivity coating applied to its left end face to function as the total reflection mirror within the resonant cavity. Upon achieving a pump power of 37 W at a repetition rate of 12 kHz, the system produced a maximum average power of 32 mW, with a pulse width varying from 2.48 ns to 2.70 ns and a central wavelength of 228.5 nm, which is effectively applicable for deep-ultraviolet spectral detection.

Non-invasive neurostimulation: an emerging therapeutic modality in veterinary neurology

Non-invasive neurostimulation involves techniques that stimulate the nervous system without penetrating the skin. These techniques, which range from electrical and magnetic to other types of stimulation, are designed to alter the activity of nerve cells and the broader networks they form. Although these methods have proven effective and safe for treating certain neurological conditions in humans, their success varies significantly from one individual to another. This variability can be because of the lack of standardised stimulation protocols, biological differences among individuals and the particular state of the neural areas being targeted. Various non-invasive neurostimulation techniques have been investigated, particularly in human medicine, including transcutaneous vagal nerve stimulation, transcutaneous electrical nerve stimulation on various nerves, repetitive transcranial magnetic stimulation and transcranial direct current stimulation. Of these, repetitive transcranial magnetic stimulation has emerged as the leading technique in research for human and veterinary medicine because of its potential in treating a wide range of neurological disorders. In the field of veterinary medicine, the use of non-invasive neurostimulation as a treatment approach is still in its early phases, with only a few reports documenting its application in managing seizures and behavioural issues in animals.

Endocrine management of transgender and gender diverse adolescents: expert opinion of the ESPE Working Group on Gender Incongruence and the Endo-ERN main thematic group on Sexual Development and Maturation.

Increasing numbers of transgender and gender diverse adolescents seek endocrine treatment to align their body to their gender identity. In this relatively young field of medicine, there is a limited body of evidence, and the available evidence generally is of low quality. However, in the absence of randomised trials, much can be learned from prospective observational studies. While evidence on somatic and psychosocial outcomes has increased in recent years, areas with little or no research rely on expert opinion. In order to reduce practice variation in paediatric endocrine care for transgender adolescents, we describe endocrine care for transgender and gender diverse adolescents in Europe in this statement, based on the available evidence as well as the experience of experts from the ESPE Working Group on Gender Incongruence and from the European Reference Network Endo-ERN, main thematic group 'Sexual Development and Maturation'. This document aims to provide practical information and tools for clinical care, with sections on the multidisciplinary team, counseling, fertility, hormone suppression, gender affirming hormone treatment, support for non-binary adolescents, long-term follow up and transition of care, altered treatment wishes, lifestyle and sexual health, and in addition includes a paragraph on ethical considerations regarding this care.

Obstacles to the Development of the Use of Artificial Intelligence from the Point of View of Physicians Working in Selected AJA Medical Centers: A Qualitative Content Analysis Study

Background: Considering the increasing use of decision support systems, specifically artificial intelligence (AI), in the field of medicine, our goal in this study was to identify, from the perspective of physicians working in AJA medical centers, the obstacles hindering the adoption and use of AI in the treatment and diagnosis of patients. Objectives: This study investigated the obstacles faced by specialist physicians working in AJA medical centers regarding the implementation of AI in their professional practice. Methods: A qualitative approach using contractual content analysis was employed. Data were collected through semi-structured, in-depth interviews and analyzed using the qualitative analysis method of Granheim and Lundman (2004) from May to July 2022. The study included 20 physicians working in selected AJA medical centers in Tehran. Sampling was conducted based on entry criteria and targeted to ensure maximum diversity. Results: The study involved 20 specialist physicians (15 men and 5 women) from selected AJA medical centers in Tehran, with an average age of 42 years and an average work experience of 14 years. The identified obstacles to the development of AI in these medical centers were categorized into three main groups: Extra-organizational and organizational factors, individual factors, and educational factors. These categories included 10 subcategories: Sanctions and economic issues, organizational attitudes, rules and regulations, available facilities, quality of facilities, knowledge and attitude, individual resistance to change, education quality, educational curriculum, and clinical professors. Conclusions: Iran's unique conditions pose challenges to the development and implementation of AI in its medical centers. This study highlights the need to address these obstacles by revising organizational rules and regulations and educating stakeholders on the benefits of AI. These measures could promote the use of AI in military medical centers and improve the quality of care.

Nano - Based Therapeutic Strategies in Management of Rheumatoid Arthritis.

Rheumatoid arthritis (RA) is a chronic autoimmune disease, progressively distinctive via cartilage destruction, auto-antibody production, severe joint pain, and synovial inflammation. Nanotechnology represents as one of the utmost promising scientific technologies of the 21st century. It exhibits remarkable potential in the field of medicine, including imaging techniques and diagnostic tools, drug delivery systems and providing advances in treatment of several diseases with nanosized structures (less than 100 nm). Conventional drugs as a cornerstone of RA management including disease-modifying antirheumatic drugs (DMARDS), Glucocorticosteroids, etc are under clinical practice. Nevertheless, their low solubility profile, poor pharmacokinetics behaviour, and non-targeted distribution not only hamper their effectiveness, but also give rise to severe adverse effects which leads to the need for the emergence of nanoscale drug delivery systems. Several types of nano-diagnostic agents and nanocarriers have been identified; including polymeric nanoparticles (NPs), liposomes, nanogels, metallic NPs, nanofibres, carbon nanotubes, nano fullerene etc. Various patents and clinical trial data have been reported in relevance to RA treatment. Nanocarriers, unlike standard medications, encapsulate molecules with high drug loading efficacy and avoid drug leakage and burst release before reaching the inflamed sites. Because of its enhanced targeting specificity with the ability to solubilise hydrophobic drugs, it acts as an enhanced drug delivery system. This study explores nanoparticles potential role in RA as a carrier for site-specific delivery and its promising strategies to overcome the drawbacks. Hence, it concludes that nanomedicine is advantageous compared with conventional therapy to enhanced futuristic approach.

Study on the physicochemical properties and immune regulatory mechanism of polysaccharide fraction from Aronia Melanocarpa fruit.

Aronia Melanocarpa (Michx.) Elliott fruit has been extensively used in the food and medicinal fields. This study aimed to analyze the physicochemical properties of a polysaccharide fraction (AMP2) isolated from this fruit for the first time and investigated its immune regulatory mechanism. The physicochemical properties of AMP2 were determined using high-performance gel permeation chromatography, PMP derivatization-high performance liquid chromatography, Ultraviolet spectroscopy, and infrared spectroscopy. The metagenomic technology was applied to investigate the regulatory effects and mechanisms of AMP2 on the gut microbiota of immunosuppressed mice. The results showed that molecular weight of AMP2 was 83,444Da, which was mainly composed of D-arabinose, D-xylose, D-mannose, D-rhamnose and D-glucose, and both β-type and α-type glycosidic bonds contained in its structure. AMP2 changed the composition of gut microbiota by increasing the number of beneficial and probiotic bacteria, thereby regulated the intestinal mucosal immune system of host. AMP2 improved intestinal immune system response and antimicrobial capacity through positive regulation of the NOD-like receptor signaling pathway and neutrophil extracellular trap formation. The results demonstrate the potential of AMP2 in immune regulation, providing a new perspective for its subsequent development and contributing to the development and application of related health foods.

The Surgeon's Role in Disaster and Counterterrorism Medicine.

Medical and surgical care remain a critical feature of disaster preparedness. The evolution of modern warfare introduces elements manifesting as both domestic and foreign terrorism. The fields of disaster and counterterrorism medicine continue to evolve in response to these looming threats. While perhaps not common, education, preparedness, and medical/surgical capabilities are paramount to an effective disaster and counterterrorism medical (and surgical) response. The present study examines the specific role of the surgeon within the scope of disaster and counterterrorism medicine.

Data mining applications in risk research: A systematic literature review

Despite the rising literature on data mining (DM) approaches, there is a lack of a complete literature review and categorization system within risk research. This paper presents the first recognized academic literature review on the application of data mining tools in risk research provides an up-to-date SCOPUS literature database. Based on bibliometric analysis, 5422 papers related torisk were identified from a total of 77,410 studies on data mining and thoroughly analyzed. Each of the selected 5422 papers was classified into four risk categories: global risk, public health risk, molecular and biomedical risk, and pharmaceutical risk. Each primary risk category was further subdivided to highlight the specific research focuses within each domain. Global risks encompass business, environmental, and social risks. Scholars have predominantly focused on the banking, market, and construction sectors within business risk, while environmental risk includes catastrophe-related risks. Social risks encompass areas such as education, traffic safety, and transportation concerns. Clinical data is usually employed in public health risk research, while various radiomic databases are utilized in genetic and molecular biology research. In pharmaceutical research, DM is primarily used to detect adverse drug effects. According to the findings of this review, the fields of computer science and medicine received the most significant research attention. The review also discusses limitations and provides a roadmap to guide future research, aiming to enhance knowledge development related to the application of data mining techniques in risk-related studies.

CT-based radiomics predicts adjacent vertebral fracture after percutaneous vertebral augmentation.

Adjacent vertebral fracture (AVF) is a frequent complication following percutaneous vertebral augmentation (PVA). While radiomics is widely utilized in the field of spinal medicine, its application for assessing the risk of AVF in post-PVA patients remains limited. We aim to develop and validate predictive models using machine learning algorithms for radiomics and clinical risk factors to assess the risk of AVF after PVA. This retrospective study included 158 patients with osteoporotic vertebral compression fractures who underwent PVA at our hospital, of which 48 patients had AVF within 2years. The patients were divided into train and test cohorts in a ratio of 7:3. Radiomics features of the surgically intervened vertebrae were extracted from CT images, and selected using Mann-Whitney U-test and LASSO regression to construct a radiomic signature. Machine learning algorithms (SVM and LR) were then employed to integrate the radiomics signature with clinical data to develop predictive models. The performance of the model was assessed using Receiver Operating Characteristic (ROC) curves and calibration curves. Nine optimal radiomics features were selected to form the radiomics model, while five clinical features were identified for the clinical model. The AUCs of the radiomics, clinical, and combined models developed using the SVM algorithm were 0.77, 0.77, and 0.83 on the test cohort, and those of the LR algorithm were 0.78, 0.81, and 0.86. Radiomics and machine learning modeling using postoperative CT images demonstrate noteworthy capability in assessing the risk of AVF following PVA.

Three-Dimensional Bioprinting for Retinal Tissue Engineering

Three-dimensional bioprinting (3DP) is transforming the field of regenerative medicine by enabling the precise fabrication of complex tissues, including the retina, a highly specialized and anatomically complex tissue. This review provides an overview of 3DP’s principles, its multi-step process, and various bioprinting techniques, such as extrusion-, droplet-, and laser-based methods. Within the scope of biomimicry and biomimetics, emphasis is placed on how 3DP potentially enables the recreation of the retina’s natural cellular environment, structural complexity, and biomechanical properties. Focusing on retinal tissue engineering, we discuss the unique challenges posed by the retina’s layered structure, vascularization needs, and the complex interplay between its numerous cell types. Emphasis is placed on recent advancements in bioink formulations, designed to emulate retinal characteristics and improve cell viability, printability, and mechanical stability. In-depth analyses of bioinks, scaffold materials, and emerging technologies, such as microfluidics and organ-on-a-chip, highlight the potential of bioprinted models to replicate retinal disease states, facilitating drug development and testing. While challenges remain in achieving clinical translation—particularly in immune compatibility and long-term integration—continued innovations in bioinks and scaffolding are paving the way toward functional retinal constructs. We conclude with insights into future research directions, aiming to refine 3DP for personalized therapies and transformative applications in vision restoration.

Marine Bacteria: A Source of Novel Bioactive Natural Products.

Marine natural products have great pharmacological potential due to their unique and diverse chemical structures. The marine bacterial biodiversity and the unique marine environment lead to a high level of complexity and ecological interaction among marine species. This results in the production of metabolic pathways and adaptation mechanisms that are different from those of terrestrial organisms, which has drawn significant attention from researchers in the field of natural medicine. This review provides an analysis of the distribution and frequency of keywords in the literature on marine bacterial natural products as well as an overview of the new natural products isolated from the secondary metabolites of marine bacteria in recent years. Finally, it discusses the current research hotspots in this field and speculates on future directions and limitations.

CTGCT, Centre of Excellence for the Technologies of Gene and Cell Therapy: Collaborative Translation of Scientific Discoveries into Advanced Treatments for Neurological Rare Genetic Diseases and Cancer

The emerging field of precision medicine relies on scientific breakthroughs to understand disease mechanisms and develop cutting-edge technologies to overcome underlying genetic and functional aberrations. The establishment of the Centre of Excellence for the Technologies of Gene and Cell Therapy (CTGCT) at the National Institute of Chemistry (NIC) in Ljubljana represents a significant step forward, as it is the first centre of its kind in Slovenia. The CTGCT is poised to spearhead advances in cancer immunotherapy and personalised therapies for neurological and other rare genetic diseases. The centre’s overarching mission is to extend beyond the NIC’s scientific excellence in basic research and bring new therapeutic solutions toward clinical application. The CTGCT aims to develop a broad pipeline of biomedical tools, including innovative synthetic biology tools, gene editing and splicing technologies, RNA-based technologies, immune regulation engineering and novel viral and non-viral delivery systems. The CTGCT is supported by partner institutions from the UK, the Netherlands and Germany, which already have academic good manufacturing practice (GMP) facilities for the manufacture of advanced therapy medicinal products (ATMPs) and is committed to active collaboration with clinicians and patient organizations at all stages of development to improve access to gene and cell therapies (GCTs) for patients. The Centre also seeks to collaborate with national and international academic and industrial partners, and the newly established GMP facilities will address a critical bottleneck in the translation of GCTs from research to practice. Finally, CTGCT's translational research and technology transfer units will ensure the impactful dissemination of research and innovation activities in Slovenia, throughout the Western Balkans and Eastern Europe region, and beyond. With its comprehensive approach and forward-looking vision, the CTGCT will drive transformative advances in gene and cell therapies for the benefit of patients on a global scale.