Medicinal Substances Research Articles

Multianalytical investigation reveals psychotropic substances in a ptolemaic Egyptian vase.

This study presents a comprehensive multimodal analytical study of an Egyptian ritual Bes-vase, of the 2nd century BCE employing cutting-edge proteomics, metabolomics, genetics techniques, and synchrotron radiation-based Fourier Transformed Infrared microSpectroscopy (SR µ-FTIR) to characterize organic residues of its content. We successfully identified the presence of various functional, bioactive, psychotropic, and medicinal substances, shedding light on the diverse components of a liquid concoction used for ritual practices in Ptolemaic Egypt. Using LC-MS/MS with a new methodological approach, we identified key proteins and metabolites, enabling the identification of botanical sources, confirmed by genetic sequences. Our analyses revealed traces of Peganum harmala, Nimphaea nouchali var. caerulea, and a plant of the Cleome genus, all of which are traditionally proven to have psychotropic and medicinal properties. Additionally, the identification of human fluids suggests their direct involvement in these rituals. Furthermore, metabolomics and SR µ-FTIR analyses also revealed the presence of fermented fruit-based liquid and other ingredients such as honey or royal jelly. The identification of specific chemical compounds, such as alkaloids and flavonoids, provides insight into the psychoactive and therapeutic uses of these in ancient ritual practices. This multidisciplinary study highlights the complexity of ancient cultures and their interactions with psychoactive, medicinal, and bioactive substances. These findings contribute to our understanding of ancient belief systems, cultural practices, and the utilization of natural resources, ultimately enhancing our knowledge of past societies and their connection to the natural world.

Oceanic Breakthroughs: Marine-Derived Innovations in Vaccination, Therapy, and Immune Health

The vast, untapped potential of the world’s oceans is revealing groundbreaking advancements in human health and vaccination. Microalgae such as Nannochloropsis spp. and Dunaliella salina are emerging as resources for recombinant vaccine development with specific and heterologous genetic tools used to boost production of functional recombinant antigens in Dunaliella salina and Nannochloropsis spp. to induce immunoprotection. In humans, several antigens produced in microalgae have shown potential in combating diseases caused by the human papillomavirus, human immunodeficiency virus, hepatitis B virus, influenza virus, Zika virus, Zaire Ebola virus, Plasmodium falciparum, and Staphylococcus aureus. For animals, microalgae-derived vaccine prototypes have been developed to fight against the foot-and-mouth disease virus, classical swine fever virus, vibriosis, white spot syndrome virus, and Histophilus somni. Marine organisms offer unique advantages, including the ability to express complex antigens and sustainable production. Additionally, the oceans provide an array of bioactive compounds that serve as therapeutics, potent adjuvants, delivery systems, and immunomodulatory agents. These innovations from the sea not only enhance vaccine efficacy but also contribute to broader immunological and general health. This review explores the transformative role of marine-derived substances in modern medicine, emphasizing their importance in the ongoing battle against infectious diseases.

Cytotoxic Effects of Plant Secondary Metabolites and Naturally Occurring Bioactive Peptides on Breast Cancer Model Systems: Molecular Mechanisms

Breast cancer is the second leading cause of death among women, and the number of mortal cases in diagnosed patients is constantly increasing. The search for new plant compounds with antitumor effects is very important because of the side effects of conventional therapy and the development of drug resistance in cancer cells. The use of plant substances in medicine has been well known for centuries, but the exact mechanism of their action is far from being elucidated. The molecular mechanisms of cytotoxicity exerted by secondary metabolites and bioactive peptides of plant origin on breast cancer cell lines are the subject of this review.

Formulation and Evaluation of Clotrimazole Mucoadhesive Vaginal Globules

The aim of this study was to prepare vaginal suppositories with mucoadhesive properties to prolong the action of antifungal component clotrimazole (CLO). This was achieved by preparing vaginal pessaries on a hydrophilic gel base composed of gelatin and gelatin enriched with PEG 400 (in a 1:1 ratio), and then checking the properties of the obtained vaginal drugs. The prepared globules, containing 100 mg of CLO, were characterized in terms of mass and swelling index, organoleptic analysis was also prepared. In addition, a texture analysis and a study of the dissolution of clotrimazole were performed. On the basis of the obtained results, it was concluded that the modification of the composition of the gelatin–glycerin base by the addition of macrogol had a positive effect on the mucoadhesive properties of the globules. In addition, due to the presence of PEG 400, the globules were stiffer. It was also observed that the presence of CLO reduces the value of the force needed for compression during the texture analysis study. Comparing the CLO release profiles of the prepared globules and commercially available clotrimazole tablets, the release profile for the globules was slower than for the tablets, which indicates the possibility of using mucoadhesive globules as a form of a drug that releases the medicinal substance more slowly at the site of administration.

Decoding eggplant fruit: Multi-omics profiling of caffeoyl-CoA-3-OMT expression

Eggplant (Solanum melongena L.) is a rich source of health-promoting phenolic acids, mainly chlorogenic acid (CGA), and trace elements. CGA has been shown to have antioxidant, anti-inflammatory, neuroprotective, cardioprotective, anticancer, and antidiabetic properties. The natural genome of eggplants contains essential genes for beneficial traits like drought tolerance, disease resistance, and high concentrations of medicinal substances. Transcriptomic analysis of eggplant overexpressing the hydroxycinnamoyl CoA quinate hydroxycinnamoyl transferase (HQT) gene revealed upregulation of 2165 genes, including three similar to HQT and cinnamate-4-hydroxylase (C4H). Sequence similarity analysis showed homology to caffeoyl-CoA O-methyltransferases, key enzymes in the phenylpropanoid pathway leading to CGA biosynthesis. Gene ontology and KEGG pathway analysis identified 5 highly upregulated glycosyltransferase family 43 genes (650.3-fold change). Glycosyltransferases like UDP-glucose:cinnamate glucosyltransferase and cinnamate-glucose 4′-O-glucosyltransferase are crucial for CGA production in eggplant. qRT-PCR validated the potential upregulation of HQT and C4H in transgenic eggplants. Comparative analysis revealed eggplant has the highest CGA content (5–8.1 g/kg dry weight) among vegetables, with foliar CGA acting as a natural insecticide. CGA offers antioxidant, hypoglycemic, antiviral, and hepatoprotective benefits to humans. This study highlights the role of HQT and C4H in elevating the medicinal properties of eggplant through CGA biosynthesis pathway engineering.

Formulation of Ethanol Extract Cream of Sikkam Leaves (Bischofia javanica Blume) as an Antifungal Against Candida albicans

Skin health issues in Indonesia, including those caused by fungal infections such as (Candida albicans), can lead to various conditions like candidiasis, scabies, tungiasis, and leprosy. This study aims to evaluate the potential of sikkam leaf extract in developing an antifungal cream to combat (Candida albicans). Fungi are eukaryotic organisms that cannot photosynthesize and reproduce through branching filaments composed of hyphae. Fungi can thrive in diverse environments such as water, soil, and decaying organic matter. A cream is a semi-solid emulsion preparation in which one or more medicinal substances are dissolved or dispersed within a base. This formulation determines the cream’s effectiveness, including the optimal ethanol extract concentration needed for antifungal activity. Extraction of sikkam leaves was conducted using maceration with 70% ethanol, and creams were formulated with extract concentrations of 5%, 10%, and 15%. Data were analyzed using the Kruskal-Wallis test. The study results showed that sikkam leaf-based creams meet quality standards, including organoleptic properties, homogeneity, pH, spreadability, and physical stability. Effectiveness testing indicated that creams with 5%, 10%, and 15% concentrations produced inhibition zones against (Candida albicans) with average diameters of 6.3 mm, 9 mm, and 15.3 mm, respectively. The higher the extract concentration, the stronger the inhibition of fungal growth. The cream with a 15% concentration proved to be the most effective, with inhibition classified as strong. Thus, sikkam leaf extract shows potential as a key ingredient for antifungal cream formulations.

Binding studies of ruthenium complexes with antithyroid drug

Medicinal substances are essential to our daily existence. A prudent approach to metal complexation with already available medications may result in more stylish, intelligent, and effective drug compositions. Thus, the binding of [Ru(NN)3]2+ (NN = 2,2ˈ-bipyridine, 1,10-phenanthroline) with the antithyroid medication thyronorm has been studied in the current attempt. The use of absorption and emission spectrum techniques allowed researchers to examine the complexes' interactions with antithyroid medications. Studies of absorption and emission spectra show that the complex and medication interact through coordinated ligand hydrophobic and hydrogen bonding interactions.Analysis has also been done on the binding of the antithyroid medication Thyronorm to ruthenium complexes. The Benesi-Hildebrand plot was used to determine the binding constants of the drug complexes. Current research indicates that the Thyronorm pill has good affinity with [Ru(phen)3]2+complex.

Low pharmaceutical pollution of epibenthic organisms from Admiralty Bay, Antarctica (King George Island, South Shetland Islands)

The presence of medicinal products has been reported in the Antarctic marine environment but still little is known about the bioaccumulation of these compounds. This study was set up to detect and quantify pharmaceutical residues in benthic biota from Admiralty Bay, King George Island (South Shetland Islands, Antarctica). Pharmaceuticals and stimulants were analysed using LC-MS/MS in dominant epibenthic macroorganisms that were collected at eight nearshore sublittoral sites (water depth 3–30 m) by a SCUBA diving team during austral summer (February 2023). Out of 22 analytes, only the stimulant caffeine (CAF) and the antiepileptic carbamazepine (CBZ) have been identified in the macrobenthic algae and invertebrates indicating a relatively low contamination level of the bay. The analytes were detected predominantly in the northern part of the bay (Mackellar Inlet and Martel Inlet) which reflects likely their elevated environmental concentrations in this area and suggests that local research stations represent the main source of pharmaceutical contamination. Irrespective of the sampling site, both compounds were found almost exclusively in brown and red macroalgae highlighting their potential for uptake and accumulation of CAF and CBZ. This study provided the first evidence of the presence of medicinal substances in the Antarctic macrobenthic organisms and can serve as a baseline for environmental risk assessment.

A novel immunopharmacological biocompound

The publication is devoted to topical issues of experimental study of new biocompounds, based on the creation of a consortium of biological composite compounds – biological active substances (metabiotics) produced by strains 59T and 60T of saprophytic compounds of the genus Bacillus subtilis. These strains are very promising for the creation of hepatopicts, new medicinal substances, in the creation and development of a new pharmacological class of hepatoprotectors. Previous studies have shown the safety and hepatoprotective effect of biologically active drugs. It is worth noting that the very effective compounds are the produced biologically active substances – metabolites of bacterial origin, on the basis of which it seems appropriate to create a new class of drugs – metabiotics. The absence of vegetative probiotic cells in such compounds will reduce the additional immune load on the body. The combined use of these compounds provides a potentiated pharmacological effect. In this regard, it seemed appropriate, under the conditions of modeling toxic liver damage by carbon tetrachloride, to conduct an experimental assessment of the immunotropic effect of biologically active substances (BAS) on laboratory animals in order to confirm the effect on cellular factors of immunity. The purpose of the study was to study the effect of the combined use of dietary supplements produced by Bacillus subtilis microorganisms on indicators of cellular immunity in laboratory animals with toxic liver damage. Acute toxic hepatitis was reproduced in white laboratory rats with repeated intragastric administration of carbon tetrachloride. The comparison drug was a registered hepatoprotective drug – ursosan. The immunotropic effect was assessed using factors such as: phagocytic activity of macrophages and neutrophils (FA), NBT test, quantitative assessment of antibody-forming cells, T and B lymphocytes. As a result of the studies performed, reliable data were obtained on an increase in the number of T and B lymphocytes, antibody-forming cells, as well as an increase in FA, which confirms the activation of all parts of cellular immunity in conditions of acute toxic liver damage. The data obtained allow us to recommend the studied biocompound for the creation of new drug candidates of microbiological origin, hepatoprotective agents with immunotropic effects.

Regulation of hepatic transporters OATP1A2 and OATP1B1 by the action of nitric oxide (II)

Nitric oxide II (NO) is a signaling molecule that has a wide range of physiological effects, including the regulation of gastrointestinal processes. The liver actively expresses the clinically significant transporters OATP1A2 and OATP1B1, which are involved in the influx of biologically active and medicinal substances. That is why it seems relevant to determine the pathways of regulation of hepatic transporters under the influence of NO. Aim. To study the effect of NO on the relative amount and expression of the transporters OATP1A2 and OATP1B1 in vitro in HepG2 cells. Materials and methods. The study was performed on a culture of HepG2 cells, which were cultured in 6-well plates at 37 °C and 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) with a high glucose content (4500 mg/l) containing L-glutamine (4 mM), 10% fetal bovine serum, 100 U/ml penicillin and 100 mg/ml streptomycin (all components from Sigma-Aldrich, Germany). S-nitrosoglutathione (Sigma-Aldrich, Germany) was added to the culture medium at concentrations of 1, 10, 50, 100 and 500 µM, incubated for 24 and 72 hours. Water for injection (solvent) was added to control cells in an equivalent volume S-nitrosoglutathione). The relative amounts of OATP1A2 and OATP1B1 proteins were assessed by Western blot, and the expression of SLCO1A2 and SLCO1B1 by real-time PCR. The results of the study. In the course of this study, it was shown that the addition of S-nitrosoglutathione in the concentration range of 10-500 μM and exposure duration of 24 and 72 hours causes an increase in the intracellular level of nitric oxide metabolites, which indicates the adequacy of the use of this NO donor. At the same time, under the influence of NO, there was an increase in the relative amount of the studied transporters - OATP1A2 at an exposure period of 24 hours and S-nitrosoglutathione concentrations of 50 and 100 μM, OATP1B1-24 and 72 hours, at concentrations of 10-500 μM, a similar trend was noted for the expression genes SLCO1A2 and SLCO1B1. Conclusion. The NO donor - S-nitrosoglutathione causes an increase in the relative amount of OATP family transporters - OATP1A2 and OATP1B1, due to increased expression of the SLCO1A2 and SLCO1B1 genes, in vitro in HepG2 cells.

Hydration behavior of D-calcium pantothenate (vitamin B5) in the presence of sugar-based deep eutectic solvents at different temperatures: experimental and theoretical study

Considerable efforts have been devoted in recent years to enhancing the efficacy medicinal substance, leading to the discovery of innovative drug formulations and delivery techniques. The successful design of these processes necessitates a profound understanding at the molecular level of how these substances interact with biological membranes. Thorough thermodynamic investigations provide invaluable insights into these interactions and aid in selecting suitable compounds for pharmaceutical production. This study aims to determine the density and speed of sound for D-calcium pantothenate in mixtures of water and deep eutectic solvents (DESs), specifically choline chloride/sucrose, choline chloride/ glucose, and choline chloride/ fructose (with 2:1 molar ratio) over a temperature range of 288.15 K to 318.15 K under atmospheric pressure. In order to predict the behavior of molecules, COSMO model (the Conductor-Like Screening Model) offer complementary strengths in quantum chemistry. This approach allows for calculating solvation free energies, making it ideal for predicting properties like solubility, where understanding solvent-solute interactions is crucial. By correlating the measured parameters using standard relationships, important partial molar parameters such as apparent molar volumes and apparent molar isentropic compressibility are calculated. Additionally, apparent molar isobaric expansion, and Hepler’s constant are derived from the density and speed of sound data. The experimental apparent molar volumes, and apparent molar isentropic compressibility data is fitted to the Redlich-Meyer equation to obtain significant quantities such as standard partial molar volume, and partial molar isentropic compression. The comprehensive thermodynamic analysis of this studied system holds immense significance for advancements in the pharmaceutical industry.

Short Lysine-Containing Tripeptide as Analgesic Substance: The Possible Mechanism of Ligand–Receptor Binding to the Slow Sodium Channel

A possible molecular mechanism of the ligand–receptor binding of Ac-Lys-Lys-Lys-NH2 (Ac-KKK-NH2) to the NaV1.8 channel that is responsible for nociceptive signal coding in the peripheral nervous system is investigated by a number of experimental and theoretical techniques. Upon Ac-KKK-NH2 application at 100 nM, a significant decrease in the effective charge carried by the NaV1.8 channel activation gating system Zeff is demonstrated in the patch-clamp experiments. A strong Ac-KKK-NH2 analgesic effect at both the spinal and supraspinal levels is detected in vivo in the formalin test. The distances between the positively charged amino groups in the Ac-KKK-NH2 molecule upon binding to the NaV1.8 channel are 11–12 Å, as revealed by the conformational analysis. The blind docking with the NaV1.8 channel has made it possible to locate the Ac-KKK-NH2 binding site on the extracellular side of the voltage-sensing domain VSDI. The Ac-KKK-NH2 amino groups are shown to form ionic bonds with Asp151 and Glu157 and a hydrogen bond with Thr161, which affects the coordinated movement of the voltage sensor up and down, thus modulating the Zeff value. According to the results presented, Ac-KKK-NH2 is a promising candidate for the role of an analgesic medicinal substance that can be applied for pain relief in humans.

Effects of alkaline salt stress on growth, physiological properties and medicinal components of clonal Glechoma longituba (Nakai) Kupr.

BackgroundGlechoma longituba, recognized as a medicinal plant, provides valuable pharmaceutical raw materials for treating various diseases. Saline-alkali stress may effectively enhance the medicinal quality of G. longituba by promoting the synthesis of secondary metabolites. To investigate the changes in the primary medicinal components of G. longituba under saline-alkali stress and improve the quality of medicinal materials, Na2CO3 was applied to induce short-term stress under different conditions and the biomass, physiologically active substances and primary medicinal components of G. longituba were measured in this study.ResultsUnder alkaline salt stress, the activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD), and ascorbate peroxidase (APX) were elevated in G. longituba, accompanied by increased accumulation of proline (Pro) and malondialdehyde (MDA). Furthermore, analysis of the medicinal constituents revealed that G. longituba produced the highest levels of soluble sugars, flavonoids, ursolic acid, and oleanolic acid under 0.6% Na2CO3 stress for 48 h, 0.2% Na2CO3 stress for 72 h, 0.4% Na2CO3 stress for 12 h, and 0.4% Na2CO3 stress for 8 h, respectively.ConclusionsShort-term Na2CO3 stress enhances the synthesis of medicinal components in G. longituba. By manipulating stress conditions, the production of various medicinal substances could be optimized. This approach may serve as a basis for the targeted cultivation of G. longituba, offering potential applications in the treatment of diverse diseases.

ACUTE TOXICITY OF 5-((5-BROMOFURAN-2-YL)-4-METHYL-3-THIOHEPTYL)-1,2,4-TRIAZOLE

Statistical data show that more than 130,000 people in Ukraine are diagnosed with cancer every year. Cancer can affect any organ and eventually the whole body. In most cases, cancer is a tumor affecting epithelial, mucous, muscle, fat, and bone tissues. Also, processes related to malignant formations can occur in the blood and lymph. The main mechanism that triggers the process of formation of neoplasms is a violation of growth and, accordingly, cell division. They begin to grow uncontrollably, multiply, and can also migrate, spreading to nearby tissue structures, and later degenerate into atypical (malignant) ones. Cancer is one of the most life-threatening diseases. Literature data show that more than 90% of cancer patients die due to chronic metastases. Cancer therapy involves the use of drugs that block the growth and spread of cancer cells by affecting certain molecular structures involved in the growth, progression, and spread of cancer. The diversity of the use of means for cancer therapy, namely, hormone therapy, the use of angiogenesis inhibitors and apoptosis inducers, has provided scientists with various options for finding effective means to fight this disease. Heterocyclic compounds are the most common among organic substances. All of them are of natural or synthetic origin and play an extremely important role in human life. A scientifically proven fact today is the high biological activity of 1,2,4-triazole derivatives, they are also used as plasticizers for plastics, anti-corrosion agents, additives to various types of fuels, plant protection agents, etc. Derivatives of 1,2,4-triazole are well known as biologically active compounds, some of them are active substances of medicines. Scientists in many countries of the world are studying the properties of 1,2,4-triazole derivatives. The spectrum of pharmacological activity of 1,2,4-triazoles is wide enough, which allows scientists to choose different directions of research tests. It is also known that 1,2,4-triazole derivatives are low-toxic or practically non-toxic compounds. Studying the toxicity of biologically active molecules is a primary task on the way to their introduction into practical medicine. It was previously established that 5-((5-bromofuran-2-yl)-4-methyl-3-thioheptyl)-1,2,4-triazole has anticancer activity, acting selectively and purposefully on cancer cells. Therefore, it was necessary to investigate the acute toxicity of 5-((5-bromofuran-2-yl)-4-methyl-3-thioheptyl)-1,2,4-triazole by the method of experimental research and using computer technologies. Materials and methods. To achieve this aim, we used both classical and modern methods. Timely well-known methods for computer prediction of acute toxicity GUSAR (Germany), ProTox 3.0 (Germany), TEST (USA) and pkCSM (Australia). To obtain more reliable results, we also used the V. B. Prozorovsky express method in vivo on white nonlinear rats. Results. The data obtained for predicting acute toxicity using all four computer services have similar values. It should also be noted that the experimental results correspond to the obtained non-experimental data. Therefore, the compound 5-((5-bromfuran-2-yl)-4-methyl-3-thioheptil)-1,2,4-triazole can be attributed to the fourth class of toxicity according to the classification of K. K. Sydorov. Conclusions. According to generalized data, the compound 5-((5-bromfuran-2-yl)-4-methyl-3-thioheptil)-1,2,4-triazole has an LD50 value of 1245 mg/kg and is a low-toxic substance.

Fullerenes in dentistry: A review on unlocking their therapeutic potential in oral medicine

Fullerenes are a family of carbon allotropes that have demonstrated great potential in oral medicine. They have a distinct cage-like structure and take the shape of hollow spheres, ellipsoids, or tubes with special structural and physicochemical features. When fullerenes are spherical, they are known as buckyballs, and when they are cylindrical, they are known as carbon nanotubes or buckytubes. Due of their flexible construction, they can be used in a variety of settings, including medication delivery, gene therapy, diagnostics, and oral illness treatment. Fullerenes have the ability to conjugate or encapsulate medicinal substances, allowing for the regulated and targeted administration of drugs to tissues afflicted by diseases including mouth cancer and periodontal disease. When treating infections and long-term inflammatory disorders in the oral cavity, their capacity to improve drug stability and bioavailability is very advantageous. Functionalized fullerenes improve biosensing platforms and imaging methods in diagnostics, facilitating the early detection of illnesses and oral malignancies. There are still obstacles to overcome in order to maximize their biocompatibility, toxicity profiles, and delivery methods for clinical application, despite their enormous potential. This review aims in revolutionizing the detection and treatment of numerous oral disorders, and ongoing research endeavours to fully investigate the therapeutic potential of fullerenes in oral healthcare.

Impact of the Formality Level on the Strategic Planning Process of SMEs

The article identifies the barriers that hinder the implementation of strategic planning and the relationship with the level of formality in the preparation of strategic plans of SMEs in Quito. It focuses on the manufacturing sector and its different sub-sectors, including the manufacture of food products, textiles, clothing, as well as chemical, pharmaceutical, medicinal, and botanical substances for pharmaceutical use, in addition to rubber and plastic products. The existing literature reviewed on strategic planning and tools to address the process characterizes the small and medium-sized manufacturing sector as the object of research. The subsequent field study shows the results achieved by the companies with planning and the existence or not of barriers to strategic implementation. The most common barriers have been identified in relation to factors such as resources, culture, lack of technological support, among others. Once the barriers have been identified, they are linked to the use of planning tools to determine the type of existing relationship. There is a moderate correlation between the level of complexity and the level of execution of the strategic plan. The research results will enable further research on the importance of the use of management tools in SMEs.

CHITOSAN AS A DRUG CARRIER

The review discusses the latest issues related to using chitosan, a biocompatible and biodegradable polymer derived from chitin, as a carrier in intelligent drug-delivery systems. The continuous development of medicine and rapid technological progress pose new challenges in designing new therapeutic agents. Chitosan has properties that make it suitable for drug-delivery applications, such as its ability to form nanoparticles and its mucoadhesion that enhance drug absorption. Chitosan can be combined with antibiotics, analgesics and other ingredients with specific properties. While chitosan is not medicinal substance, it can be a carrier in controlled drug-release systems.

PRESERVING NUTMEG : HISTORICAL SIGNIFICANCE, MEDICINAL BENEFITS, AND CLIMATE CHANGE THREATS TO INDONESIAN NUTMEG

Over the past thousand years, scholars and practitioners, drawing from diverse fields including culture, philosophy, sociology, and history, have meticulously documented the intricacies of various medicinal substances and their applications. In this continuum of knowledge, nutmeg, a quintessential spice of Indonesian heritage, has held a prominent place. This study aims to explore the historical significance of nutmeg in Indonesia, its role in medicinal practices, and the threats posed by climate change to nutmeg cultivation. The research seeks to provide valuable insights into the cultural and historical importance of nutmeg in Indonesian society, as well as its medicinal benefits. Additionally, the study aims to analyze the potential impact of climate change on nutmeg production in Indonesia. The study uncovered nutmeg's diverse significance in Indonesia across historical, cultural, medicinal, and economic realms. It emphasized nutmeg's medicinal potential and the climate change challenges to its production. Findings stressed the necessity of robust climate policies and proactive adaptation strategies to ensure nutmeg farming sustainability. In conclusion, this study highlights nutmeg's historical, medicinal, and climate change-related challenges in Indonesia. It stresses the necessity of comprehensive measures to address climate change impacts and integrate resilience strategies into nutmeg farming practices and policymaking. These findings offer valuable guidance for policymakers and stakeholders to ensure the sustainable growth of Indonesian nutmeg exports amidst environmental changes.

Nanomedicine Advancements in Cancer Therapy: A Scientific Review

Cancer nanomedicines, characterized by submicrometer-sized formulations, aim to optimize the biodistribution of anticancer drugs by minimizing off-target effects, reducing toxicity, enhancing target site accumulation, and improving overall efficacy. Numerous nanomedicines have been developed to improve the effectiveness and safety of traditional anticancer treatments. These include formulations with carbon nanotubes, nanodiamonds, enzyme-responsive nanoparticles for controlled drug release, dendrimers as nanoparticle drug carriers, quantum dot nanocarrier systems for precise drug delivery, solid lipid nanoparticles, and polymeric nanoparticles designed for targeted drug delivery. Additionally, nanotechnology has been explored in cancer treatment through gene therapy. Despite these advances, the complex nature of carrier materials and functional integration presents challenges in preparing these candidates for clinical translation. Nanotechnology, with its unique features at the nanoscale, offers novel possibilities for developing cancer therapies while increasing efficacy and safety. Although only a few nanotherapeutics have obtained clinical approval, exciting uses for nanotechnology are on the horizon. Nanoparticles possess unique transport, biological, optical, magnetic, electrical, and thermal capabilities due to their small size within the light wavelength spectrum. This results in high surface area-to-volume ratios, allowing for the incorporation of various supporting components in addition to active medicinal substances. These properties aid in solubilization, degradation protection, delayed release, immune response evasion, tissue penetration, imaging, targeted distribution, and triggered activation. In summary, the future of nanomedicine holds promise for introducing innovative platforms in cancer treatment. The research presented underscores the potential for nanoparticles to revolutionize anticancer therapies, enhancing the overall therapeutic approach.

A COMPREHENSIVE REVIEW ON ETHOSOMAL GEL

Ethosomal systems are newer lipid vesicular carriers that have been around for 20 years, but over that period they have grown significantly as a means of transdermal drug delivery. They have a sizable amount of ethanol in them. These nanocarriers carry medicinal substances with various physicochemical qualities throughout the skin and deep skin layers. Since they were created in 1996, ethosomes have undergone substantial investigation; new substances have been added to their original composition, creating new varieties of ethosomal systems. These innovative carriers, which can be added to gels, patches, and lotions, are prepared using several novel methods. In addition to clinical trials, many in vivo models are employed to assess the effectiveness of dermal/transdermal administration. This review focuses on different generation of ethosomes and their comparison with other conventional liposomes. KEY WORDS: Ethosomes, Transdermal drug delivery, Lipid vesicular systems,