Transmission Mechanism Research Articles

Religious Leaders’ Compliance with State Authority: Experimental Evidence from COVID-19 in Pakistan

Abstract A randomized controlled trial in Pakistan tests whether one-on-one engagement with community religious leaders can encourage them to instruct congregants to follow government regulations. Treated religious leaders are 25 percent more likely to comply with government requirements to tell congregants they should wear a mask to prevent COVID transmission when attending prayers. Treatment effects do not depend on the religious content of the message. Effects are driven by respondents who already understand the mechanics of COVID transmission at baseline, suggesting the treatment does not work by correcting basic knowledge about the disease, but rather through a mechanism of persuasion.

Design Method of Cam Steering Mechanism Based on Path Fitting

In order to improve the accuracy of a solar-powered punch card car’s movement on a designated route and reduce positional deviations during its operation, a solar-powered punch card car with a single cam as the steering guidance mechanism was designed. The car adopts a three-wheel structure. The transmission mechanism, steering mechanism, driving mechanism, and regulating mechanism of the car were analyzed. The kinematics model of the car was established and the motion characteristics of the car were obtained. By analyzing the relationship between the steering angle of the car and the curvature radius of its travel route, the front wheel angle of the car at each position was calculated using MATLAB R2020a. This allowed us to establish the relationship between the front wheel angle and the displacement of the steering push rod, which was further converted into the theoretical contour line of the cam. Subsequently, the theoretical contour line of the cam was completed and envelope correction was performed. Finally, through mechanical analysis and experimental verification using a prototype, the results indicated that the single-cam steering guidance mechanism calculated using this fast path fitting method exhibited excellent mechanical performance and a smooth and accurate trajectory, and the traveling path of the theoretical cam contour curve was basically consistent with the actual trajectory route.

SUSTAINABLE DIGITAL TRANSFORMATION: THE NEXUS BETWEEN ICT AND GLOBAL GREEN ECONOMIC GROWTH

As a new engine to promote global economic and social development, information and communication technology (ICT) plays a key role in the field of modern economy. The aim of this paper is to investigate the nexus between ICT and green total factor productivity (GTFP) on a global scale. An extended data envelopment analysis model (DEA), named WINDOWS-US-SBM, was constructed for calculating the GTFP of 65 countries from 2007 to 2019. This paper empirically analyzes the spatial effect and the transmission mechanism of ICT development on GTFP in countries from different income groups. The results show a prominent imbalance between ICT development and GTFP in various countries. ICT can effectively improve GTFP and play a crucial role in lower middle-income countries. The development of ICT can improve GTFP through technological progress, energy intensity, and trade openness. This paper is helpful to provide policy guidance for the development of ICT and give a new perspective of global green development.

Adipose stem cells-derived small extracellular vesicles transport Thrombospondin 1 cargo to promote insulin resistance in gestational diabetes mellitus

BackgroundGestational diabetes mellitus (GDM) is a highly prevalent disease and poses a significant risk to the health of pregnant women. Abdominal adipose tissue (AT) contributes to insulin resistance (IR) associated with GDM. However, the underlying mechanisms remain unclear.MethodsIn this study, we developed a mouse model of GDM by subjecting mice to a high-fat diet. We collected adipose-derived stem cells (ADSCs) from the abdominal and inguinal regions and examined their role in inducing IR in normal tissues through the secretion of small extracellular vesicles (sEVs). The sEVs derived from ADSCs isolated from GDM mice (ADSC/GDM) were found to inhibit cell viability and insulin sensitivity in AML12, a normal mouse liver cell line.ResultsThrough proteomic analysis, we identified high levels of the thrombospondin 1 (Thbs1) protein in the sEVs derived from ADSC/GDM. Subsequent overexpression of Thbs1 protein in AML12 cells demonstrated similar IR as observed with ADSC/GDM-derived sEVs. Mechanistically, the Thbs1 protein within the sEVs interacted with CD36 and transforming growth factor (Tgf) β receptors in AML12 cells, leading to the activation of Tgfβ/Smad2 signaling. Furthermore, the administration of LSKL, an antagonistic peptide targeting Thbs1, suppressed Thbs1 expression in ADSC/GDM-derived sEVs, thereby restoring insulin sensitivity in AML12 cells and GDM mice in vivo.ConclusionsThese findings shed light on the intercellular transmission mechanism through which ADSCs influence hepatic insulin sensitivity and underscore the therapeutic potential of targeting the Thbs1 protein within sEVs.

Mammalian orthoreovirus capsid protein σ3 antagonizes RLR-mediated antiviral responses by degrading MAVS.

Mammalian orthoreovirus (MRV) is an important zoonotic pathogen, but the regulatory role of its viral proteins in retinoic acid-inducible gene-like receptor (RLR)-mediated antiviral responses is still poorly understood. Herein, we show that MRV σ3 protein co-localizes with mitochondrial antiviral signaling protein (MAVS) in the mitochondria and promotes the mitochondria-mediated intrinsic apoptotic pathway to cleave and consequently degrade MAVS. Furthermore, tryptophan at position 133 of σ3 protein plays a key role in the degradation of MAVS. Importantly, we show that MRV outer capsid protein σ3 is a key factor in antagonizing RLR-mediated antiviral responses, providing evidence to better unravel the infection and transmission mechanisms of MRV.

Evaluating the Impact of Low-Carbon Urban Policy on Corporate Green Innovation—Evidence from China’s National Low-Carbon City Strategy Program

Low-carbon urban policy (LCUP) and corporate green innovation are considered crucial strategies and methods for reducing urban carbon emissions, addressing climate change, and promoting urban environmental sustainability. This study constructed a quasi-natural experiment based on the low-carbon city strategy program implemented in China in 2010, utilizing data from Chinese prefecture-level cities and publicly listed companies from 2005 to 2020. Employing a multi-period difference-in-differences (DID) approach, this paper reveals that the establishment of low-carbon model cities effectively fosters green innovation in corporations. Further analysis demonstrates that this promotional effect is particularly significant in non-state-owned enterprises, enterprises with high media attention, those with a high level of digitalization, and enterprises located in cities with high levels of green finance and in the Eastern and Central regions of China. These conclusions withstood a series of robustness tests, confirming their validity. Meanwhile, the examination of policy mechanisms reveals that public environmental awareness, government environmental regulation, and corporate environmental information disclosure are three key policy transmission mechanisms through which LCUP affects corporate green innovation. The findings of this study provide significant empirical insights for addressing climate change and enhancing the sustainable capacity of urban environments.

Sweet complexity: O-linked protein glycosylation in pathogenic Neisseria

Sweet complexity: O-linked protein glycosylation in pathogenic Neisseria

Semiconservative transmission of DNA N6-adenine methylation in a unicellular eukaryote.

While DNA N6-adenine methylation (6mA) is best known in prokaryotes, its presence in eukaryotes has generated great interest recently. Biochemical and genetic evidence supports that AMT1, a MT-A70 family methyltransferase (MTase), is crucial for 6mA deposition in unicellular eukaryotes. Nonetheless, 6mA transmission mechanism remains to be elucidated. Taking advantage of Single Molecule Real-Time Circular Consensus Sequencing (SMRT CCS), here we provide definitive evidence for semiconservative transmission of 6mA in Tetrahymena thermophila In wild-type (WT) cells, 6mA occurs at the self-complementary ApT dinucleotide, mostly in full methylation (full-6mApT); after DNA replication, hemi-methylation (hemi-6mApT) is transiently present on the parental strand, opposite to the daughter strand readily labeled by 5-bromo-2'-deoxyuridine (BrdU). In ΔAMT1 cells, 6mA predominantly occurs as hemi-6mApT. Hemi-to-full conversion in WT cells is fast, robust, and processive, while de novo methylation in ΔAMT1 cells is slow and sporadic. In Tetrahymena, regularly spaced 6mA clusters coincide with linker DNA of nucleosomes arrayed in the gene body. Importantly, in vitro methylation of human chromatin by reconstituted AMT1 complex recapitulates preferential targeting of hemi-6mApT sites in linker DNA, supporting AMT1's intrinsic and autonomous role in maintenance methylation. We conclude that 6mA is transmitted by a semiconservative mechanism: full-6mApT is split by DNA replication into hemi-6mApT, which is restored to full-6mApT by AMT1-dependent maintenance methylation. Our study dissects AMT1-dependent maintenance methylation and AMT1-independent de novo methylation, reveals a 6mA transmission pathway with striking similarity to 5-methyl cytosine (5mC) transmission at the CpG dinucleotide, and establishes 6mA as a bona fide eukaryotic epigenetic mark.

The Effect of the Fed Interest Rate Hikes on the Financial Crisis Evidence from Emerging Market Economices

The purpose of this paper is to systematically study the economic consequences of this policy adjustment by analysing in depth the five interest rate hike actions of the Federal Reserve and their chain reactions in emerging market economies, applying economic principles and methods. In particular, the central question of concern is whether the Fed's interest rate hiking policy constitutes an important trigger of the financial crisis in emerging markets. In order to shed light on this complex phenomenon in a comprehensive manner, this paper not only analyses in depth the macroeconomic logic behind the Fed's interest rate hike, but also explores its transmission mechanism in emerging market economies, as well as the economic vulnerability of these countries in the face of external shocks. The results of the study show that the collapse of emerging countries as a result of the Fed's interest rate hikes seems to be inevitable, but we can draw insights from it: actively promoting the reform of the international monetary system and supporting the inclusion of more types of currencies in the international trade and investment settlement system has become a common choice, including for some Western countries, as well as the need to continue to optimise the economic structure of the domestic economy and to strengthen the ability to manage and control the comprehensive risks.

A high-robustness radial intensity-orientated mode decomposition with reliable noise elimination

Mode decomposition (MD) provides profound evidence to reveal the internal modal transmission mechanism. However, the indelible noise has always been the main stubborn hindrance in practical MD. In the complex superposition case with a large number of modes, the traditional MD is not capable enough to distinguish the real modal intensity and the annoying noise, sustaining an unacceptable accuracy and fluctuation. This paper proposes a radial intensity-orientated MD (RIO-MD) method with reliable noise elimination. Our approach focuses on the inherent modal radial features in Polar coordinates, getting rid of the traditional two-dimensional image processing in Cartesian ones. The RIO-MD introduces the inherent radial intensity relationship into MD for better extracting mode coefficients. Based on the expectable real radial modal intensity, the RIO-MD enables to recognize and extraction of the three kinds of stubborn noise, including interference pattern noise, device noise, and random noise. The RIO-MD works well in mode decomposition case. The values of correlation coefficients (C) between the experimental and reconstructed image are higher than 93%. The mean square error (MSE) is lower than 3 × 10−3. Both the C and MSE keep stable, with the standard deviation 30 times lower than the other widely used methods, demonstrating the high-robustness of the RIO-MD. Due to the reliable noise recognition, the RIO-MD shows great possibility in mode number expansion.

Evaluation of the dynamics of oral pathogenic microbiota in COVID-19 patients by genomic sequencing after application of foam with antimicrobial action

Relevance. The COVID-19 pandemic caused by the SARS-CoV-2 virus has demonstrated the critical importance of understanding the mechanisms of transmission and finding effective methods of prevention. Particular attention has been paid to the role of oral hygiene, as the oral cavity serves as a major route of transmission. Studies show that the composition of the oral microbiota can influence the course and outcome of COVID-19 disease. Therefore, there is a need to study the effects of oral antiseptics on the microbiome, which may offer new opportunities for the prevention and treatment of this disease.Objective. The aim of this study is to evaluate the effectiveness of the oral cleansing foam «Parodontol PROF» in reducing the pathogenic microflora of the oral cavity in patients with COVID-19 and to assess its effect on the risk of secondary infections.Materials and methods. The study was conducted on the basis of the Clinical Medical Centre «Kuskovo» of Russian University of Medicine. It included 450 patients with a confirmed diagnosis of COVID-19. The participants were divided into two groups: the test group used oral cleansing foam, while the control group used no additional oral hygiene. Comparative analyses of the taxonomic composition of the oropharyngeal microbiota and dental plaque before and after the use of the cleansing foam were performed.Results. The study showed that patients who regularly used the cleansing foam «Parodontol PROF» had a significant decrease in the representation of pathogens, including Mycoplasma, and an increase in the number of beneficial commensals such as Lactococcus and Lactobacillus. These changes indicate the potential efficacy of this product to improve oral hygiene and reduce the risk of secondary infections.Conclusion. The study supports the hypothesis that improving oral hygiene with specialised antiseptic agents, such as a cleansing foam, may help to reduce pathogenic microflora in the oral cavity in patients with COVID-19. This, in turn, may reduce the risk of pathogen migration into the lower respiratory tract and the development of secondary infections. The findings emphasise the importance of further research in this area and the development of comprehensive approaches to oral hygiene management in the context of infectious diseases.

Characterization of linezolid- and methicillin-resistant coagulase-negative staphylococci in a tertiary hospital in China

BackgroundRecently, linezolid-resistant staphylococci have become an emerging problem worldwide. Understanding the mechanisms of resistance, molecular epidemiology and transmission of linezolid-resistant CoNS in hospitals is very important.MethodsThe antimicrobial susceptibilities of all isolates were determined by the microdilution method. The resistance mechanisms and molecular characteristics of the strains were determined using whole-genome sequencing and PCR.ResultsAll the strains were resistant to oxacillin and carried the mecA gene; 13 patients (36.1%) had prior linezolid exposure. Most S. epidermidis and S. hominis isolates were ST22 and ST1, respectively. MLST typing and evolutionary analysis indicated most linezolid-resistant CoNS strains were genetically related. In this study, we revealed that distinct CoNS strains have different mechanisms of linezolid resistance. Among ST22-type S. epidermidis, acquisition of the T2504A and C2534T mutations in the V domain of the 23 S rRNA gene, as well as mutations in the ribosomal proteins L3 (L101V, G152D, and D159Y) and L4 (N158S), were linked to the development of linezolid resistance. In S. cohnii isolates, cfr, S158Y and D159Y mutations in the ribosomal protein L3 were detected. Additionally, emergence of the G2576T mutation and the cfr gene were major causes of linezolid resistance in S. hominis isolates. The cfr gene, G2576T and C2104T mutations, M156T change in L3 protein, and I188S change in L4 protein were found in S. capitis isolates.ConclusionThe emergence of linezolid-resistant CoNS in the environment is concerning because it involves clonal dissemination and frequently coexists with various drug resistance mechanisms.

An optimized cooperative data transmission scheme in relay‐enabled cellular communication networks

SummaryCooperative transmission is of great significance in data transmission and resource schedule in mobile cellular communication networks. To attain the desired network transmission performance objectives over the whole cellular network system, we propose an appropriate cooperative data transmission scheme to optimize the data delivery efficiency in terms of the transmission time consumption for each and every transmission group. The proposed transmission optimization scheme includes the tasks of determining the transmission group, allocating the channel rate resource, specifying the cooperative transmission mechanism, and performing resource allocation and schedule as well. Firstly, we propose a utility function based rate allocation scheme to optimize the channel rate resource utilization for all those transmission groups over the cellular communication network. Based on it, we further present a cooperative data transmission scheme to deal with the data delivery and reception for all those subgroups with high efficiency over the whole network system. Particularly, we propose a resource efficiency function‐based coordination scheduling (EFCS) scheme to conduct the cooperative data transmission and resource schedule as well for each transmission group within the network. The EFCS scheme facilitates to determine the subgroups for schedule over each scheduling cycle, which can produce the best data transmission efficiency across both the direct and cooperative transmission hop. Both the theoretical analysis and the simulation evaluation results verify the superior performance of our proposed cooperative data transmission scheme.

Mechanism of stress transmission and feasibility of mining the lower coal seam under repeated mining in close-multiple coal seams

Mining in an upper coal seam results in the redistribution of vertical stress within a lower coal seam until a new equilibrium state is attained. Close-multiple coal seams mining faces the occurrence of multi-goafs and a complex stress environment. Based on a case study of close-multiple coal seams with repeated mining in the Qianjiaying coal mine, a stress distribution model of the floor in the coal seam striking range was established by optimizing the load form of the abutment pressure. The floor stress state after primary and repeated mining of upper coal seams was analyzed in a visual and quantitative manner. The concentrations of vertical stress, horizontal stress, and shear stress are all located in the floor under the coal rib after primary and repeated mining. The curve of stresses gradually levels off as the depth of the floor increases, indicating a diminishing influence of mining-induced stress from the upper coal seam. Based on the stress analysis by numerical simulations, the overall lower coal seam remains in a state of large-scale stress relief. Stress relief occurred twice under repeated mining, which indicates that the lower coal seam has favorable mining feasibility. The results of the study can provide scientific guidance to prevent mining accidents.

China's environmental tax reform achieves the co-management of carbon and haze: The role of optimizing energy utilization

Environmental tax reform in China is an essential initiative to achieve carbon and haze co-management by shifting the Environmental Fee to Tax (EFT). Therefore, we employ a Coupling Coordination Degree (CCD) model to measure the level of carbon and haze co-management across three distinct scenarios in China. Treating the EFT policy as a quasi-natural experiment, the study explores its impact on carbon and haze co-management using data of 282 prefecture-level cities in China during 2015–2020. Furthermore, the study further analyzes the regional heterogeneity and the transmission mechanisms of optimizing energy utilization. The principal findings of this investigation are as follows. First, the co-management levels of carbon and haze in China show a discernible upward trend from 2015 to 2020. A heightened emphasis on carbon reduction is helpful in improving the co-management level of carbon and haze. Co-management levels of carbon and haze are lower within the treatment group compared to the control group, but their growth rates are higher. Second, the EFT policy in China has significantly enhanced the co-management level of carbon and haze. Third, the impact of China's EFT policy has a more pronounced effect in western China and a comparatively weaker effect in middle China, which may be attributed to variations in industrial infrastructure, supporting facilities, availability of clean energy resources, and prevailing policy inclinations across different regions of China. Finally, China's EFT policy is an instrumental factor in augmenting the co-management level of carbon and haze, primarily through pathways of upgrading energy structure and improving energy efficiency.

Large-scale bacterial genomic and metagenomic analysis reveals Pseudomonas aeruginosa as potential ancestral source of tigecycline resistance gene cluster tmexCD-toprJ

BackgroundThe global dissemination of the multidrug resistance efflux pump gene cluster tmexCD-toprJ has greatly weakened the effects of multiple antibiotics, including tigecycline. However, the potential origin and transmission mechanisms of the gene cluster remain unclear. MethodsHere, we concluded a comprehensive bioinformatics analysis on integrated 73,498 bacterial genomes, including Pseudomonas spp., Klebsiella spp., Aeromonas spp., Proteus spp., and Citrobacter spp., along with 1,152 long-read metagenomic datasets to trace the origin and propagation of tmexCD-toprJ. ResultsOur results demonstrated that tmexCD-toprJ was predominantly found in Pseudomonas aeruginosa sourced from human hosts in Asian countries and North American countries. Phylogenetic and genomic feature analyses showed that tmexCD-toprJ was likely evolved from mexCD-oprJ of some special clones of P. aeruginosa. Furthermore, metagenomic analysis confirmed that P. aeruginosa is the only potential ancestral bacterium for tmexCD-toprJ. A putative mobile genetic structure harboring tmexCD-toprJ, int-int-hp-hp-tnfxB-tmexCD-toprJ, was the predominant genetic context of tmexCD-toprJ across various bacterial genera, suggesting that the two integrase genes play a pivotal role in the horizontal transmission of tmexCD-toprJ. ConclusionsBased on these findings, it is almost certain that the tmexCD-toprJ gene cluster was derived from P. aeruginosa and further spread to other bacteria.

Update on human herpesvirus 7 pathogenesis and clinical aspects as a roadmap for future research.

Human herpesvirus 7 (HHV-7) is a common virus that is associated with various human diseases including febrile syndromes, dermatological lesions, neurological defects, and transplant complications. Still, HHV-7 remains one of the least studied members of all human betaherpesviruses. In addition, HHV-7-related research is mostly confined to case reports, while in vitro or in vivo studies unraveling basic virology, transmission mechanisms, and viral pathogenesis are sparse. Here, we discuss HHV-7-related literature linking clinical syndromes to the viral life cycle, epidemiology, and viral immunopathogenesis. Based on our review, we propose a hypothetical model of HHV-7 pathogenesis inside its host. Furthermore, we identify important knowledge gaps and recommendations for future research to better understand HHV-7 diseases and improve therapeutic interventions.

Presence of phage-plasmids in multiple serovars of Salmonella enterica.

Evidence is accumulating in the literature that the horizontal spread of antimicrobial resistance (AMR) genes mediated by bacteriophages and bacteriophage-like plasmid (phage-plasmid) elements is much more common than previously envisioned. For instance, we recently identified and characterized a circular P1-like phage-plasmid harbouring a bla CTX-M-15 gene conferring extended-spectrum beta-lactamase (ESBL) resistance in Salmonella enterica serovar Typhi. As the prevalence and epidemiological relevance of such mechanisms has never been systematically assessed in Enterobacterales, in this study we carried out a follow-up retrospective analysis of UK Salmonella isolates previously sequenced as part of routine surveillance protocols between 2016 and 2021. Using a high-throughput bioinformatics pipeline we screened 47 784 isolates for the presence of the P1 lytic replication gene repL, identifying 226 positive isolates from 25 serovars and demonstrating that phage-plasmid elements are more frequent than previously thought. The affinity for phage-plasmids appears highly serovar-dependent, with several serovars being more likely hosts than others; most of the positive isolates (170/226) belonged to S. Typhimurium ST34 and ST19. The phage-plasmids ranged between 85.8 and 98.2 kb in size, with an average length of 92.1 kb; detailed analysis indicated a high amount of diversity in gene content and genomic architecture. In total, 132 phage-plasmids had the p0111 plasmid replication type, and 94 the IncY type; phylogenetic analysis indicated that both horizontal and vertical gene transmission mechanisms are likely to be involved in phage-plasmid propagation. Finally, phage-plasmids were present in isolates that were resistant and non-resistant to antimicrobials. In addition to providing a first comprehensive view of the presence of phage-plasmids in Salmonella, our work highlights the need for a better surveillance and understanding of phage-plasmids as AMR carriers, especially through their characterization with long-read sequencing.

Monetary Policy and Bank Risk-taking

As the process of global financial integration deepens, the relationship between bank risk-taking and monetary policy has become increasingly close. In recent years, financial markets have been highly volatile and banking risks have become increasingly prominent. Against this background, the relationship between monetary policy and bank risk-taking in China is studied through both theoretical and empirical evidence, and corresponding policy recommendations are put forward. In addition, the role mechanism of monetary policy affecting bank risk is deeply explored through the theoretical aspect of monetary policy and bank risk capacity bearing, and the theoretical basis for the formulation of related policies is provided. Therefore, it explores the impact of bank risk on the transmission mechanism of monetary policy from the theory, and further improves the theory of monetary policy transmission to enhance the effectiveness and stability of monetary policy. The article analyzes and discusses the impact of monetary policy on bank risk-taking capacity, to provide a reference for the development of the financial industry.

Research on the Performance of Lightweight Prefabricated Concrete Stairs with a Special-Shaped Hollow Landing Slab

In order to further improve the technical advantages of lightweight prefabricated concrete stairs, a kind of prefabricated stair system using a special-shaped hollow landing slab was proposed. Based on the detailed structural composition display, the design method for the main components (prefabricated flight and special-shaped prefabricated hollow landing slab) was proposed and a design application example was provided. Furthermore, specialized experimental and numerical simulation studies were conducted on the key component—the special-shaped prefabricated hollow landing slab. The research results indicated that this new kind of lightweight prefabricated concrete stairs using a special-shaped prefabricated hollow landing slab has reasonable construction, an effective design method, a clear force transmission mechanism, moderate component weight, and high transportation and installation convenience.