Rejuvenation Mechanism Research Articles

Shedding Light on the Rejuvenation Mechanism of Reactive Compounds in Aged SBS-Modified Asphalt through Molecular Dynamics and Quantum Chemical Simulations

Shedding Light on the Rejuvenation Mechanism of Reactive Compounds in Aged SBS-Modified Asphalt through Molecular Dynamics and Quantum Chemical Simulations

Modeling Influence of Aging and Rejuvenation Mechanisms on the Recycled Asphalt Pavement (RAP) Binder Fatigue Life through the Linear Amplitude Sweep Test

Modeling Influence of Aging and Rejuvenation Mechanisms on the Recycled Asphalt Pavement (RAP) Binder Fatigue Life through the Linear Amplitude Sweep Test

Meiosis as a mechanism for epigenetic reprogramming and cellular rejuvenation.

Meiosis is a hallmark of sexual reproduction because it represents the transition from one life cycle to the next and, in animals, meiosis produces gametes. Why meiosis evolved has been debated and most studies have focused on recombination of the parental alleles as the main function of meiosis. However, 40 years ago, Robin Holliday proposed that an essential function of meiosis is to oppose the consequence of successive mitoses that cause cellular aging. Cellular aging results from accumulated defective organelles and proteins and modifications of chromatin in the form of DNA methylation and histone modifications referred to collectively as epigenetic marks. Here, recent findings supporting the hypothesis that meiosis opposes cellular aging are reviewed and placed in the context of the diversity of the life cycles of eukaryotes, including animals, yeast, flowering plants and the bryophyte Marchantia.

Transcriptomic profiling reveals the effect of amino acid metabolism on the production capacity of Volvariella volvacea during repeated subculture

Tissue separation is commonly employed for the purification and rejuvenation of edible mushroom species. However, repeated or continuous tissue separation can result in strain degradation. In this study, cultivation experiments were conducted in Volvariella volvacea V844 (T0). Tissue separation subculture was repeated 16 times to obtain the succession strains T1–T16. Of these, T4, T8, T12, and T16 were selected as experimental strains, and their production traits were evaluated. Additionally, transcriptome sequencing technology was used to analyze the transcription spectrum of these strains. The results showed that with the increase in subculture cycles, the colony diameter, mycelial growth rate, mycelial biomass, and biological efficiency of V. volvacea first increased and then decreased, and the growth cycle was gradually prolonged. Transcriptome sequencing revealed the presence of 1375, 1015, 651, and 1648 differentially expressed genes (DEGs) in T4, T8, T12, and T16, respectively, versus T0. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis demonstrated that these DEGs were mainly involved in amino acid metabolic pathways. Further annotation analysis revealed that in V. volvacea subculture strains, the number of DEGs involved in histidine metabolism (Ko00340) and arginine and proline metabolism (Ko00330) was the highest. Amino acid analysis demonstrated that the proline and arginine content first rose and then fell across successive subculture strains, whereas the histidine content first decreased and then increased. The results indicated that the change in the production capacity of V. volvacea during repeated subculture may be due to the altered expression of DEGs involved in amino acid metabolism, leading to changes in the amino acid content of V. volvacea and further affecting the accumulation of reactive oxygen species, free radical scavenging, energy acquisition, nutrient absorption, and redox balance. These changes could result in the rejuvenation (<8) of the V. volvacea strain or cause its degradation (>12). The results of this study provide a theoretical framework for studying the mechanisms of rejuvenation and degradation in V. volvacea and also offer insights valuable for studying the molecular functions of genes involved in the growth of V. volvacea and other edible fungi.

A residence time-based concept for modeling and analyzing the impact of diffusion on auto-ignition processes with thermal stratification

A meticulous definition of the residence time (also referred to as the fluid age) in the context of auto-ignition in a thermally stratified gas has demonstrated that the age can be considered as a level-set function, which allows the tracking of a self-ignition front propagating. In order to avoid the difficulty of defining the boundary and initial conditions for this age, which can be seen as fluid birth, a normalized age is introduced. Meticulous derivation of the equation revealed additional terms related to the scalar dissipation rates and the gradient in the composition space of the auto-ignition delay. To validate this model equation, two canonical configurations were proposed: one representing a hot spot self-igniting with significant thermal diffusion and the other where a self-ignition front propagates at an almost constant speed. The analysis, based on the normalized age of the particles, reveals the impact of thermal diffusion on the acceleration or deceleration of particle aging. In certain instances, a particle rejuvenation mechanism through thermal diffusion has been identified. Furthermore, this work demonstrates the capacity of the normalized residence time field to map transitions between auto-ignition and laminar flame. Finally, the different cases studied were classified in an auto-ignition/diffusion diagram based on three non-dimensional characteristic numbers, which highlighted five combustion regimes.

REGENERATION OF SECONDARY ASPHALT PAVEMENTS WITH REJUVENATING AGENTS

Recycling reclaimed asphalt pavement fulfills an important function in achieving a sustainable and environmentally friendly road construction industry. Using reclaimed asphalt pavement (RAP) it is possible to produce new asphalt with a small amount of virgin raw materials. This will reduce the amount of energy required to extract and process virgin asphalt pavement materials, along with the amount of waste generated during road reconstruction. Despite these advantages, road agencies have been reluctant to use high proportions of RAP materials in new asphalt production because the presence of large amounts of oxidized bitumen from RAP in new mixtures makes them less workable and less compactable than mixtures with virgin bitumen alone. One should also be aware of the high stiffness of RAP mixtures, which will make them difficult to use. Various methods can be used to overcome this problem, one of the most effective is the addition of rejuvenating agents. This article describes research into the production of asphalt mixtures from recycled asphalt pavement (RAP) material. This work aims to study the effectiveness of various rejuvenators for restoring the basic performance characteristics of asphalt pavements. The most effective rejuvenators and their classification are reviewed. The hypothesized mechanism of rejuvenation of asphalt pavements by the introduction of rejuvenators is presented. The methods of introducing various rejuvenators into RAP are described.

Nuclear Magnetic Resonance (NMR) Assessment of Bio and Crude Oil-Based Rejuvenation

Asphalt binders in pavements lose their stability through aging and eventually fail in the field. Using nuclear magnetic resonance (NMR) to monitor the primary longitudinal relaxation time of asphalt samples and the ratio of material that carries this primary relaxation time has been shown to indicate the impact of ultraviolet (UV) radiation on the aging of asphalt pavements. Longitudinal NMR relaxation was used to investigate two types of proposed asphalt rejuvenators, a bio-oil-based rejuvenator and a crude-oil-based rejuvenator. Two different binders with the performance grades (PG) 64-22 and 76-22 were considered for their interactions with the rejuvenators. After 72 h of exposure to intense UV radiation, specifically designed NMR relaxometry experiments were applied to compare the rejuvenation capabilities of the two rejuvenator samples. The crude oil-based rejuvenator was found to exhibit relaxation times similar to the binder samples while the bio-based material showed relaxation times that pointed to different nuclear hydrogen environments. Both rejuvenators reduced the primary relaxation time of the PG 76-22 binder, which indicates that their stiffness was reduced. Both types of rejuvenators also seemed to prevent the effects of UV aging. Two mechanisms of rejuvenation were identified by NMR relaxometry. The primary relaxation time can be used to indicate a change in stiffness while the primary ratio of the material is tied to oxidative aging. Oxidative aging creates distinct hydrogen environments due to asphaltene aggregation. The bio-based rejuvenator only reduced the binder’s stiffness, while the crude oil-based rejuvenator also reduced the aggregation of asphaltenes. Consequently, the bio-based rejuvenator could be classified as an asphalt softener, while the oil-based material acted like a true rejuvenator.

Unique Energy‐Storage Behavior Driven by High Entropy in Metallic Glasses

AbstractThe rejuvenation or energy‐storage behavior in metallic glasses (MGs) has been extensively explored for its theoretical and practical significance. However, very limited research focuses on the rejuvenation of high entropy metallic glasses (HEMGs), leaving uncertainties about how configurational entropy influences this process. In this study, cryogenic temperature cycling (CTC) is utilized to unlock the rejuvenation potential of a series of La‐based MGs with different entropy values. Comparative analysis of these MGs reveals that increased entropy boosts the maximum rejuvenation degree from 37% to 65% and widens the range of energy states where rejuvenation occurs. The changed rejuvenation behavior induced by entropy increase, which has never been reported other studies, are related to glass's structures, fragility and coupling degree between β and α relaxations. Besides, a new rejuvenation mechanism is revealed in HEMGs, which differs significantly from unrestricted growth and interconnection of flow units observed in most MGs. The atomic motion in HEMGs is confined within the icosahedral backbone network during CTC. Surprisingly, this local motion can propagate through strong interactions between adjacent atoms, facilitating the activation of α relaxation. These discoveries offer novel approaches to tune energy‐storage behavior of MGs by modulating icosahedral network structures through entropy control.

Mechanism of Rejuvenation in Aged SBS-Modified Asphalt by Density Functional Theory

As a large area of SBS-modified asphalt pavement entered the maintenance period, the aging and rejuvenation of SBS-modified asphalt have attracted attention in recent years. In order to further study the rejuvenation of aged SBS-modified asphalt, both rheological experiments and quantum mechanical simulations were used. Complex shear modulus (G*) and phase angle (δ) were used to analyze the rejuvenation effect of aged SBS-modified asphalt. Electron density, binding energy (Ebinding), and charge transfer number (Qtransfer) were used to observe the process of rejuvenation in aged SBS-modified asphalt. The results show that compared to asphalt components, SBS polymer was the least stable and most susceptible to breaking. After oxidative aging, SBS polymer with its aging products could impair the rejuvenation in aged asphalt. The interaction between aromatic components in rejuvenator and asphaltenes in aged asphalt was unstable and could be influenced by asphalt aging level. The interaction between heavy component molecules in aged asphalt with saturate component molecules in rejuvenator are closer than those with aromatic components molecules. The binding energies between saturate components in rejuvenator and asphaltenes in aged asphalt could be served as an evaluation indicator of rejuvenation.

Energy metabolism as therapeutic target for aged wound repair by engineered extracellular vesicle.

Aging skin, vulnerable to age-related defects, is poor in wound repair. Metabolic regulation in accumulated senescent cells (SnCs) with aging is essential for tissue homeostasis, and adequate ATP is important in cell activation for aged tissue repair. Strategies for ATP metabolism intervention hold prospects for therapeutic advances. Here, we found energy metabolic changes in aging skin from patients and mice. Our data show that metformin engineered EV (Met-EV) can enhance aged mouse skin repair, as well as ameliorate cellular senescence and restore cell dysfunctions. Notably, ATP metabolism was remodeled as reduced glycolysis and enhanced OXPHOS after Met-EV treatment. We show Met-EV rescue senescence-induced mitochondria dysfunctions and mitophagy suppressions, indicating the role of Met-EV in remodeling mitochondrial functions via mitophagy for adequate ATP production in aged tissue repair. Our results reveal the mechanism for SnCs rejuvenation by EV and suggest the disturbed energy metabolism, essential in age-related defects, to be a potential therapeutic target for facilitating aged tissue repair.

Evaluation of the rheological properties and molecular distributions of asphalt binder under multiple aging and rejuvenating cycles (MARC)

Because of the widespread use of reclaimed asphalt pavement (RAP), various recycling agents (RAs) are developed and used in pavement engineering nowadays to restore the properties of RAP in asphalt mixes. Whereas there are many RA products available, it remains unclear on the fundamental rejuvenation mechanisms behind different RAs and the selection method of proper RA product to restore these recycled materials properties. In addition, there are important questions on hold in terms of RAP repeated recycling and the continuity of restoration capacity from these RAs. Therefore, this study is designed to track the properties changes of the asphalt binders in the context of multiple aging and rejuvenating cycles (MARC). Three different types of RA products are selected and evaluated using rheological characterization and chromatography (molecular distribution) analysis. Results show that the beneficial effects from RAs degrade over time with increase of aging/rejuvenating cycles. Two oils with their molecules that are mainly distributed in the medium molecular size (MMS) range, which compensate the loss of the MMS molecules of the base binder due to aging, show the good effectiveness in restoring not only the complex modulus but also the phase angle of the aged asphalt. And the good correlations between phase angle and MMS as well as LMS proportion indicate the fundamental relationship between binder’s molecular distribution and its rheological properties.

Initiation phase cellular reprogramming ameliorates DNA damage in the ERCC1 mouse model of premature aging.

Unlike aged somatic cells, which exhibit a decline in molecular fidelity and eventually reach a state of replicative senescence, pluripotent stem cells can indefinitely replenish themselves while retaining full homeostatic capacity. The conferment of beneficial-pluripotency related traits via in vivo partial cellular reprogramming in vivo partial reprogramming significantly extends lifespan and restores aging phenotypes in mouse models. Although the phases of cellular reprogramming are well characterized, details of the rejuvenation processes are poorly defined. To understand whether cellular reprogramming can ameliorate DNA damage, we created a reprogrammable accelerated aging mouse model with an ERCC1 mutation. Importantly, using enhanced partial reprogramming by combining small molecules with the Yamanaka factors, we observed potent reversion of DNA damage, significant upregulation of multiple DNA damage repair processes, and restoration of the epigenetic clock. In addition, we present evidence that pharmacological inhibition of ALK5 and ALK2 receptors in the TGFb pathway are able to phenocopy some benefits including epigenetic clock restoration suggesting a role in the mechanism of rejuvenation by partial reprogramming.

Interplay between tectonics and surface processes in the evolution of mountain ranges: Insights from landscape dynamics, uplift, and active deformation of Talesh Mountains (NW Iranian Plateau margin)

Drainage divides are dynamic features of a landscape that migrate over time during the development of river networks. In this study, we focused on the geomorphic response of a landscape to the interaction among tectonics, climate, and lithology in the Talesh Mts. along the northwest of the Iranian Plateau. The footprints of these competing forces have been analyzed by divide stability analysis and applying χ, χQ, and Gilbert metrics. Additionally, we examined the effects of bedrock erodibility on landscape evolution processes by measuring the mechanical rock strength of the lithological units in different sectors of the mountain range. The distribution of the χ values suggests a disequilibrium of the drainage networks across the whole main divide and its tendency to migrate towards the interior of the plateau. In contrast, Gilbert metrics, which focuses on a narrow topographic zone across the divide, suggest that the uppermost divide in the northern and southern Talesh Mts. is stable. Moreover, asymmetry in Gilbert metrics across the central part of the range suggests that the divide might be migrating towards the plateau interior. The possible scenario for the whole divide's behavior is associated with the contrasting erosion rates across the divide. In the northern and southern sectors of the Talesh Mts. range, the erosional wave related to the most recent uplift pulses has not reached the divide yet. Mechanism of topographic rejuvenation is controlled by heterogeneous uplift of strong bedrock units in the northern and southern sectors, and weak bedrock units outcropping in the central sector, initiated topographic rejuvenation largely in the central Talesh Mts. Parallelly, the precipitation has a limited effect in assisting and magnifying the divide mobility, reorganization, and landscape transience. In general, we can infer that divide is in a transient state due to the spatially varying rock uplift and bedrock erodibility.

A novel WCO-MDI reactive rejuvenation method for aged SBS modified asphalt toward sustainable asphalt pavements

The sustainable advancement and low-carbon application of asphalt pavement significantly benefit from the reutilization of waste SBS modified asphalt (SBSMA) mixtures. However, the high-efficiency rejuvenation of aged SBSMA (ASBSMA) remains a persistent challenge. In this study, a novel reactive rejuvenator was developed using waste cooking oil (WCO) and methylene diphenylene isocyanate (MDI) to restore asphalt components and reconstruct SBS structures in ASBSMA. Temperature Sweep, Fourier Transform Infrared (FTIR) spectroscopy and Differential Scanning Calorimetry (DSC) tests were conducted to investigate the rejuvenation effect and mechanism of ASBSMA. The results indicate that the incorporation of WCO restores the low temperature performance of ASBSMA, but adversely affects its high temperature performance. Conversely, the addition of MDI enhances the high temperature performance of ASBSMA, but an excessive dose of MDI has a negative impact on its low temperature performance. Chemical composition analysis shows that the rejuvenation by WCO is a physical dissolution and softening process, while MDI can react with the SBS aged products to produce long chain products with an amide structure and reconstruct the cross-linked structure of SBS. Although the temperature stability of rejuvenated SBSMA is not fully restored to its original state, it shows significant improvement compared with ASBSMA due to the reconstruction of SBS structure. This study effectively clarifies the reactive rejuvenation mechanism of ASBSMA, achieves the synergistic restoration of asphalt components and SBS structure, and contributes to high-efficient recycling of WCO and waste asphalt materials.

Compositional properties of planet-crossing asteroids from astronomical surveys

Context. The study of planet-crossing asteroids is of both practical and fundamental importance. As they are closer than asteroids in the Main Belt, we have access to a smaller size range, and this population frequently impacts planetary surfaces and can pose a threat to life. Aims. We aim to characterize the compositions of a large corpus of planet-crossing asteroids and to study how these compositions are related to orbital and physical parameters. Methods. We gathered publicly available visible colors of near-Earth objects (NEOs) from the Sloan Digital Sky Survey (SDSS) and SkyMapper surveys. We also computed SDSS-compatible colors from reflectance spectra of the Gaia mission and a compilation of ground-based observations. We determined the taxonomy of each NEO from its colors and studied the distribution of the taxonomic classes and spectral slope against the orbital parameters and diameter. Results. We provide updated photometry for 470 NEOs from the SDSS, and taxonomic classification of 7401 NEOs. We classify 42 NEOs that are mission-accessible, including six of the seven flyby candidates of the ESA Hera mission. We confirm the perihelion dependence of spectral slope among S-type NEOs, likely related to a rejuvenation mechanism linked with thermal fatigue. We also confirm the clustering of A-type NEOs around 1.5–2 AU, and predict the taxonomic distribution of small asteroids in the NEO source regions in the Main Belt.

Development of sustainable compound bio-oil rejuvenator and its rejuvenation mechanism on long-term aged asphalt

To develop a new sustainable compound bio-oil rejuvenator product and understand its rejuvenation mechanism on long-term aged asphalt, the castor oil vegetable asphalt (COA) was selected to react with epoxidized soybean oil (ESO) for preparing the compound bio-oil rejuvenator of long-term aged asphalt. The in-situ dynamic chemical reactions between COA and ESO, as well as the changes in basic properties, chemical compositions, molecular weight distributions and microstructures of aged asphalt were characterized before and after rejuvenation. Results indicate that COA and ESO undergo ring-opening reactions at 80 °C. These reactions still occur continuously during the rejuvenation process of aged asphalt. The compound bio-oil recovers the physical performance of long-term aged asphalt to its original level before aging. Under the combined action of COA and ESO, the sulfoxide index and carbonyl index of long-term aged asphalt samples are decreased. Molecular weight distribution of aged asphalt can be recovered with compound bio-oil. The lengths of catana phases of long-term aged base asphalt and SBS modified asphalt can be restored to their original lengths respectively. Although the average surface roughness of long-term aged base asphalt and SBS modified asphalt can be restored to the original level of base asphalt and SBS modified asphalt, it is difficult to accurately characterize the degree of asphalt rejuvenation due to the uncertainty of its changes. The developed compound bio-oil rejuvenator is suitable to restore the pavement properties of long-term aged asphalt. This is socially beneficial, economically viable and environmentally imperative.

Unraveling the nano-cracking mechanism in aged asphalt binder with consideration of rejuvenation effects

For achieving the sustainable utilization of reclaimed asphalt pavement, a popular approach is to incorporate rejuvenators to restore the cracking resistance of aged asphalt. However, research on the nanoscale rejuvenation mechanisms and the relationship with the cracking behavior is limited. To address this challenge, this study utilizes molecular dynamics simulations to explore the fundamental cracking mechanism in aged asphalt and elucidate how rejuvenators affect this process. A multiphysics analysis, including mechanical response, morphology relationship, energy evolution, deagglomeration and lubrication behavior is developed to comprehend the nano-cracking mechanism. Computational findings reveal that the cracking failure originates form cavitation, and the process primarily includes nanovoid formation, enlargement, filamentation, and separation. Oxidation enhances the aggregation of asphaltene molecules and reduces intermolecular rearrangement and mobility, which fundamentally accelerates the nano-cracking process. Owing to the diffusion of rejuvenators and their disturbances to asphaltene π-π stacking, rejuvenators can penetrate into aged asphalt molecules, lubricate and reduce intermolecular friction. This facilitates molecular rearrangement during the cracking process, thereby improving the cracking resistance and energy performance of aged asphalt binder. The finding from this study contributes to elucidating the fundamental cracking mechanism in rejuvenated asphalt binder and promoting the sustainable utilization of recycled pavement materials.

Multiscale evaluation of asphalt binder rejuvenation dosing and efficacy

The final composition of asphalt binder in an asphalt mix can vary depending if it is a neat, virgin binder, or a blend of neat binder with reclaimed asphalt pavement (RAP), modifiers, and/or rejuvenators. As recycling of asphalt concrete becomes more commonplace worldwide, so too must be the use of chemical rejuvenators which can restore the original properties of the associated asphalt binder, contributing to the performance and therefore sustainability aspect of this material. In this context, this work aimed to evaluate the influence of rejuvenation in different proportions of aged and unaged binder, as well as the best way to dose rejuvenators, taking into consideration aspects that go beyond the Superpave Performance Grading characterization of binders. In the first phase of this study, when rejuvenator contents based on low PG recovery were proportionally added to different aged/unaged binder blends, it was observed that restoration of ductility and strength of the rejuvenated aged binder was limited. However, when an additional cycle of aging was applied to simulate the new service life of recycled material, the rejuvenated recycled material generally performed better than unrejuvenated recycled material. This long term effect can better differentiate the performance of different rejuvenators. Afterwards, the dosing procedure was investigated using an extensive range of dosages. An optimal dosage determination approach was developed and verified by means of the rejuvenation of aged asphalt mixtures from the rheological, mechanical, and chemical perspectives. Finally, the differences between laboratory-aged binder and laboratory-aged mixtures when rejuvenators are used was investigated, and it was determined that the mechanisms of rejuvenation at the two scales are not completely alike. Key findings also include the importance of re-aging asphalt binder blends before testing when a rejuvenator is used and the dominance of unaged binder within binder blends with limited replacement with recycled asphalt. Overall, this work opens the door to future studies of rejuvenation of asphalt using different approaches than those which have been conventionally used, including consideration of amount of replaced binder, using nonlinear viscoelastic characterization, fundamental chemical studies, and the method of addition based on mixture versus binder scale studies.

Investigation of rejuvenation mechanisms of reclaimed asphalt rubber pavement through mortar tests

ABSTRACT Conventional rejuvenation methods recover the rheological properties of aged bitumen by adding rejuvenators to supplement the content of light fractions of bitumen. However, these methods cannot fully restore the microstructure and advantages of aged asphalt rubber (AR) binder due to the dissolution of rubber particles. Aiming at discovering a more suitable rejuvenation strategy for reclaimed asphalt rubber pavement (RARP), this study investigates the rejuvenation mechanisms of RARP through a series of chemical and rheological tests at the mortar level. A chemistry-based method was proposed to estimate the rubber dissolution in RARP mortar. AR mortars containing 40% of RARP mortar (RARP40%) were prepared. Three rejuvenation schemes were adopted for RARP40%, namely recovering the light fractions, supplementing the swelling rubber content, and both recovering and supplementing, respectively. The results indicate that adding rejuvenator only can effectively soften the aged RARP binder but it fails to slow down the rheological evolution of RARP40% mortar in the secondary aging. The incorporation of AR binder with extra rubber content notably improved the aging resistance but significantly compromised the workability of RARP40% mortar. The compound rejuvenation scheme was the optimum solution that balanced the rejuvenation effectiveness, aging resistance and workability.

Study on effect of various rejuvenators on virgin asphalt binders suffering various aging conditions and the unified evaluation index

Adding rejuvenator is a feasible method to restore the properties of aged asphalt. Due to the complex rejuvenation mechanism between different rejuvenators and aged asphalt, there is no unified standard to evaluate the rejuvenation effect of rejuvenator. Most of the current research on rejuvenator focus on the characterization of macro mechanical properties of rejuvenated asphalt binder. In order to explore the changes of chemical composition and micromechanical properties of asphalt during aging and rejuvenation, fifteen kinds of virgin asphalt samples at different aging and rejuvenation stages were prepared. A commercial rejuvenator, aromatic oil and warm mix agent were selected as rejuvenators. The fifteen asphalt samples involved various aging methods, including water-ultraviolet aging, water free ultraviolet aging, RTFOT and PAV. Through gel permeation chromatography (GPC), Fourier transform infrared spectrometer (FTIR) and atomic force microscope (AFM) test, the reversibility evaluation index Rw, RC=O, RS=O and RDMT were proposed. These indexes could be used to quantitatively evaluate the rejuvenation effects of rejuvenator on the specific properties of aged asphalt. Based on the linear fitting results among various indexes, micro reversibility index of virgin asphalt (IRIV) was proposed. The results showed that the commercial rejuvenator and warm mix agent had a limited effect on the recovery of chemical composition and molecular weight of aged asphalt. The commercial rejuvenator over restored the micro mechanical properties of aged asphalt. The effect of aromatic oil on micro mechanical properties of aged asphalt was between commercial rejuvenator and warm mix agent. This study provides a way to quantitatively evaluate the rejuvenation effect of rejuvenator on virgin asphalt binder. It is helpful to better understand the aging-rejuvenation process of virgin asphalt binder more comprehensively from chemical and micromechanical properties.