Mechanism Of Destruction Research Articles

Meet the First Authors

Meet the First Authors

Proapoptotic effect of nonthermal pulsed ultrasound on prostate cancer cells in a nude mouse model.

Ultrasound (US) can induce cell injury, and we have previously reported that adjusting the pulse repetition frequency (PRF) of ultrasound output can induce prostate cancer cell destruction without causing a rise in the temperature of the irradiated area. In this study, we examined the mechanism of nonthermal ultrasound cell destruction, which was not fully clarified in our previous reports. In vitro, we evaluated postirradiation cells immediately after treatment and examined membrane disruption by proliferation assay, LDH assay, and apoptosis assay. In vivo, we injected mice with human LNCaP and PC-3 prostate cancer cells and evaluated the therapeutic effects of US irradiation by H-E staining and immunostaining. Proliferation assays showed inhibition at 3 h postirradiation independently of PRF and cell line (p < 0.05). Quantitative assessment of apoptosis/necrosis by flow cytometry showed widely varying results depending on cell type. LNCaP showed an increase in late apoptosis at 0 h independent of PRF (p < 0.05), while PC-3 showed no significant difference at 0 h. The LDH assay showed an increase in LDH independent of PRF in LNCaP (p < 0.05 respectively), but no significant difference in PC-3. In vivo, tumor volume was compared and a significant reduction was observed at 10 Hz for LNCaP (p < 0.05) and 100 Hz for PC-3 (p < 0.001) at 3 weeks after the start of irradiation. The excised tumors were evaluated with Ki-67, Caspase-3, and CD-31 and showed a significant treatment effect independent of cell type and PRF (p < 0.001 respectively). Examining the mechanism behind the therapeutic effect of US irradiation revealed that the main effect was achieved by apoptosis induction rather than necrosis.

Relationship between the specific work of rupture and the blended ratio for two-component blended yarn

The yarn specific work of rupture is a comprehensive index to characterize the resistance of yarn to mechanical destruction. For blended yarn, it is closely related to the blended ratio of the components. This paper established a model to express the quantitative relationship between the specific work of rupture and the blended ratio in blended yarn. This model was obtained by analyzing the breaking process of blended yarn and summarizing the empirical data. Then, the experimental data were used to verify the established model and a good agreement between the calculated and experimental results was found. According to this model, the curve of the specific work of rupture against the blended ratio can be obtained by simple plotting. Consequently, this work can be used to predict the specific work of rupture for blended yarn with various blended ratios.

The Role of Hedgehog Signaling in the Melanoma Tumor Bone Microenvironment.

A crucial regulator in melanoma progression and treatment resistance is tumor microenvironments, and Hedgehog (Hh) signals activated in a tumor bone microenvironment are a potential new therapeutic target. The mechanism of bone destruction by melanomas involving Hh/Gli signaling in such a tumor microenvironment is unknown. Here, we analyzed surgically resected oral malignant melanoma specimens and observed that Sonic Hedgehog, Gli1, and Gli2 were highly expressed in tumor cells, vasculatures, and osteoclasts. We established a tumor bone destruction mouse model by inoculating B16 cells into the bone marrow space of the right tibial metaphysis of 5-week-old female C57BL mice. An intraperitoneal administration of GANT61 (40 mg/kg), a small-molecule inhibitor of Gli1 and Gli2, resulted in significant inhibition of cortical bone destruction, TRAP-positive osteoclasts within the cortical bone, and endomucin-positive tumor vessels. The gene set enrichment analysis suggested that genes involved in apoptosis, angiogenesis, and the PD-L1 expression pathway in cancer were significantly altered by the GANT61 treatment. A flow cytometry analysis revealed that PD-L1 expression was significantly decreased in cells in which late apoptosis was induced by the GANT61 treatment. These results suggest that molecular targeting of Gli1 and Gli2 may release immunosuppression of the tumor bone microenvironment through normalization of abnormal angiogenesis and bone remodeling in advanced melanoma with jaw bone invasion.

Elucidating the relationship between PFOA and PFOS destruction, particle size and electron generation in amended media commonly found in soils

Ball milling has emerged as a promising destructive technique for treating per- and polyfluoroalkyl substances (PFAS)-impacted soils. Environmental media properties such as reactive species generated upon ball milling and particle size are postulated to influence the effectiveness of the technology. In this study, four media types amended with perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) were planetary ball milled to investigate destruction, fluoride recovery without additional co-milling reagents and the relationship between PFOA and PFOS destruction, particle size during milling, and electron generation. Silica sand, nepheline syenite sand, calcite and marble were sieved to achieve similar initial particle sizes (6/35 distribution), amended with PFOA and PFOS, and milled for 4 h. Particle size analysis was conducted throughout milling and 2,2-diphenyl-1-picrylhydrazyl (DPPH•) was used as a radical scavenger to assess electron generation from the four media types. Particle size reduction was observed to be positively correlated to PFOA and PFOS destruction and DPPH• neutralization (demonstrating electron generation by milling) in silica sand and nepheline syenite sand. Milling of a fine fraction (< 500 μm) of silica sand revealed less destruction compared to the 6/35 distribution suggesting the ability to fracture grains in silicate media is integral to PFOA and PFOS destruction. DPPH• neutralization was demonstrated in all four amended media types, confirming silicate sands and calcium carbonates generate electrons as a reactive species during ball milling. Fluoride loss as a function of milling time was observed in all amended media types. A sodium fluoride (NaF) spiked was used to quantify fluoride loss in the media independent of PFAS. A method was developed using the NaF-amended media fluoride concentrations to estimate the total fluorine liberated from PFOA and PFOS by ball milling. Estimates produced suggest complete recovery of theoretical fluorine yield is obtained. Data from this study was used to propose a reductive destruction mechanism for PFOA and PFOS.

Noninvasive mechanical destruction of liver tissue and tissue decellularisation by pressure-modulated shockwave histotripsy.

Boiling histotripsy (BH) is a promising High Intensity Focused Ultrasound (HIFU) technique that can be used to mechanically fractionate solid tumours at the HIFU focus noninvasively, promoting tumour immunity. Because of the occurrence of shock scattering phenomenon during BH process, the treatment accuracy of BH is, however, somewhat limited. To induce more localised and selective tissue destruction, the concept of pressure modulation has recently been proposed in our previous in vitro tissue phantom study. The aim of the present study was therefore to investigate whether this newly developed histotripsy approach termed pressure-modulated shockwave histotripsy (PSH) can be used to induce localised mechanical tissue fractionation in in vivo animal model. In the present study, 8 Sprague Dawley rats underwent the PSH treatment and were sacrificed immediately after the exposure for morphological and histological analyses (paraffin embedded liver tissue sections were stained with H&E and MT). Partially exteriorised rat's left lateral liver lobe in vivo was exposed to a 2.0 MHz HIFU transducer with peak positive (P +) and negative (P -) pressures of 89.1 MPa and -14.6 MPa, a pulse length of 5 to 34 ms, a pressure modulation time at 4 ms where P + and P - decreased to 29.9 MPa and - 9.6 MPa, a pulse repetition frequency of 1 Hz, a duty cycle of 1% and number of pulses of 1 to 20. Three lesions were produced on each animal. For comparison, the same exposure condition but no pressure modulation was also employed to create a number of lesions in the liver. Experimental results showed that a partial mechanical destruction of liver tissue in the form of an oval in the absence of thermal damage was clearly observed at the HIFU focus after the PSH exposure. With a single pulse length of 7 ms, a PSH lesion created in the liver was measured to be a length of 1.04 ± 0.04 mm and a width of 0.87 ± 0.21 mm which was 2.37 times in length (p = 0.027) and 1.35 times in width (p = 0.1295) smaller than a lesion produced by no pressure modulation approach (e.g., BH). It was also observed that the length of a PSH lesion gradually grew towards the opposite direction to the HIFU source along the axial direction with the PSH pulse length, eventually leading to the generation of an elongated lesion in the liver. In addition, our experimental results demonstrated the feasibility of inducing partial decellularisation effect where liver tissue was partially destructed with intact extracellular matrix (i.e., intact fibrillar collagen) with the shortest PSH pulse length. Taken together, these results suggest that PSH could be used to induce a highly localised tissue fractionation with a desired degree of mechanical damage from complete tissue fractionation to tissue decellularisation through controlling the dynamics of boiling bubbles without inducing the shock scattering effect.

FIRST EVIDENCE OF COLOR PATTERNS ON CONCHS OF THE LOWER MOSCOVIAN (MIDDLE PENNSYLVANIAN) COILED NAUTILOIDS FROM THE DONETS BASIN, UKRAINE

New finds of well-preserved remains of coiled nautiloids from lower Moscovian sediments (Kamenskaya Formation) of the Donets Basin (eastern Ukraine) allowed to describe the color pattern on the conch surface of species of the genera Parametacoceras, Metacoceras, Coelogasteroceras and Ephippioceras. The conch color pattern of the studied nautiloids is represented by longitudinal light bands along the ventrolateral shoulder (Parametacoceras and Metacoceras), transverse thin lines on the venter (Ephippioceras), and a black spot on the body chamber (Coelogasteroceras). Described color patterns represent disruptive coloration. Environmental conditions, e.g. slow sedimentation, absence of agents of mechanical and chemical destruction, dysaerobic conditions and rapid burial were obviously the most important factors for the preservation of coloration on the studied conchs of coiled nautiloids.

Effects of different iodine levels on the DNA methylation of intrinsic apoptosis-associated genes and analysis of gene-environment interactions in patients with autoimmune thyroiditis.

Iodine is an essential nutrient that may change the occurrence of autoimmune thyroiditis (AIT). Apoptosis and DNA methylation participate in the pathogenesis and destructive mechanism of AIT. We detected the methylation and the expression of mRNA of intrinsic apoptosis-associated genes (YWHAG, ING4, BRSK2 and GJA1) to identify the potential interactions between the levels of methylation in these genes and different levels of iodine. 176 adult patients with AIT in Shandong Province, China, were included. The MethylTargetTM assay was used to verify the levels of methylation. We used PCR to detect the mRNA levels of the candidate genes. Interactions between methylation levels of the candidate genes and iodine levels were evaluated with multiplicative and addictive interaction models and GMDR. In the AIT group, YWHAG_1 and six CpG sites and BRSK2_1 and eight CpG sites were hypermethylated, whereas ING4_1 and one CpG site were hypomethylated. A negative correlation was found between methylation levels of YWHAG and mRNA expression. The combination of iodine fortification, YWHAG_1 hypermethylation and BRSK2_1 hypermethylation was significantly associated with elevated AIT risk. A four-locus model (YWHAG_1 × ING4_1 × BRSK2_1 × iodine level) was found to be the best model of the gene-environment interactions. We identified abnormal changes in the methylation status of YWHAG, ING4 and BRSK2 in patients with AIT in different iodine levels. Iodine fortification not only affected the methylation levels of YWHAG and BRSK2 but also interacted with the methylation levels of these genes and may ultimately increase the risk of AIT.

Recent advances of osteoimmunology research in rheumatoid arthritis: From single-cell omics approach.

Cellular immune responses as well as generalized and periarticular bone loss are the key pathogenic features of rheumatoid arthritis (RA). Under the pathological conditions of RA, dysregulated inflammation and immune processes tightly interact with skeletal system, resulting in pathological bone damage via inhibition of bone formation or induction of bone resorption. Single-cell omics technologies are revolutionary tools in the field of modern biological research.They enable the display of the state and function of cells in various environments from a single-cell resolution, thus making it conducive to identify the dysregulated molecular mechanisms of bone destruction in RA as well as the discovery of potential therapeutic targets and biomarkers. Here, we summarize the latest findings of single-cell omics technologies in osteoimmunology research in RA. These results suggest that single-cell omics have made significant contributions to transcriptomics and dynamics of specific cells involved in bone remodeling, providing a new direction for our understanding of cellular heterogeneity in the study of osteoimmunology in RA.

The effectiveness of a polyvalent antigenic complex including bacterial lysates in the treatment and prevention of rhinosinusitis against the background of acute respiratory viral infections and COVID-19 (clinical description)

One of the urgent tasks of the Ministry of Health of the Russian Federation, noted in the State report on the state of sanitary and epidemiological welfare of the population, is the improvement and implementation of diagnostic and treatment and prevention technologies. First of all, this concerns the continued growth of respiratory viral infections, seasonal influenza epidemics, where SARS-CoV-2 coronavirus infection occupies a special place, which often cause various otorhinolaryngological complications – acute and exacerbations of chronic tonsillopharyngitis, rhinosinusitis, tubotitis, purulent otitis media. In the treatment of bacterial sinusitis, in accordance with clinical recommendations, intranasal corticosteroids, elimination therapy, herbal remedies, and antibacterial drugs are used. Due to the period of the SARS-CoV-2 pandemic, a significant proportion of patients took large doses of antibacterial drugs, which caused an increase in the resistance of microorganisms to them. In this regard, it is possible to use immunostimulating drugs that stimulate cellular and humoral immunity, promote the development of a specific immune response and activate nonspecific defense factors such as lysozyme, mucin, lactoferrin and normalize mucociliary clearance. As a rule, the identification of a viral agent is carried out in case of a severe clinical course of the disease or complications from the ORL and other organs, and the use of immunostimulating agents in complex therapy is necessary at the earliest possible time of the disease. In this context, the use of immunostimulants containing polyvalent antigenic complexes, including bacterial lysates, the most common pathogens of respiratory infections, is justified. It is preferable in these cases to use lysates of bacteria obtained by mechanical destruction of bacterial cells, since they have a more pronounced non-specific immunostimulatory and specific vaccinating effect.

Study on the liquid jet and shock wave produced by a near-wall cavitation bubble containing a small amount of non-condensable gas

The liquid jet and shock wave produced by a near-wall cavitation bubble are two major reasons resulting in material-wall damage. To better understand the destructive mechanism, the complete process of action of the multi-cycle cavitation bubble collapsing on the nearby wall with the effect of non-condensable gas under different stand-off distances was numerically investigated. For this purpose, a fully compressible solver is further developed with the implementation of a modified phase-change model. Validations were conducted by comparing the numerical results with the experimental data. Furthermore, the high-pressure zones on the wall induced by the shock wave and liquid jet are carefully analyzed. Numerical results reveal that the bubble behavior, shock wave propagation, and liquid jet types are highly associated with the stand-off distance. Finally, the potential material-wall damage is estimated by using the pressure impulse, and the relationship between the pressure impulse at the central point and the stand-off distance is quantitatively analyzed. It is found two pressure impulse peaks are at the central point.

RANKL and periodontitis

Periodontal disease is characterized by inflammation of the periodontal tissue and subsequent destruction of the alveolar bone. It is one of the most common infectious diseases in humans, being the leading cause of tooth loss in adults. Recently, it has been shown that the receptor activator of NF-κB ligand (RANKL) produced by osteoblasts and periodontal ligament fibroblasts critically contributes to the bone destruction caused by periodontal disease. Activation of the immune system plays an important role in the induction of RANKL during periodontal inflammation. Here we discuss the molecular mechanisms of periodontal bone destruction by focusing on the osteoimmune molecule RANKL.

Possible Seismogenic-Trigger Mechanism of Methane Emission, Glacier Destruction and Climate Warming in the Arctic and Antarctic

Abstract-A seismogenic-trigger mechanism is proposed for the activation of methane emission on the Arctic shelf in the late 1970s, which caused the onset of a rapid climate warming in the Arctic, as well as the intensive destruction of the ice shelves of West Antarctica in the late 20th and early 21st centuries. This process is accompanied by the release of methane from the underlying hydrate-bearing sedimentary rocks and the rapid climate warming in Antarctica. The proposed mechanism is associated with the action of deformation tectonic waves in the lithosphere-asthenosphere system, caused by strong earthquakes occurring in the subduction zones closest to the polar regions: the Aleutian, located in the northern part of the Pacific Ocean, and the Chilean and Kermadec-Macquarie, located in the southeastern and southwestern parts of the Pacific lithosphere. Disturbances of the lithosphere are transmitted at an average speed of about 100 km/year over long distances of the order of 2000–4000 km, and the associated additional stresses that come to the Arctic and Antarctica several decades after earthquakes lead to the destruction of metastable gas hydrates located in the frozen rocks of the Arctic shelf or in the subglacial sedimentary rocks of Antarctica, causing the greenhouse effect of warming. Moreover, transmission of additional stresses causes a decrease in the adhesion of sheet glaciers to the underlying rocks, their accelerated sliding and the destruction of the ice sheet ice shelves in Antarctica. The considered hypothesis leads to the conclusion that in the coming decades, the processes of glacier destruction and climate warming in Antarctica will increase due to an unprecedented increase in the number of strongest earthquakes in the subduction zones of the South Pacific Ocean in the late 20th and early 21st centuries.

Focused ultrasound for treatment of peripheral brain tumors.

Malignant brain tumors are the leading cause of cancer-related death in children and remain a significant cause of morbidity and mortality throughout all demographics. Central nervous system (CNS) tumors are classically treated with surgical resection and radiotherapy in addition to adjuvant chemotherapy. However, the therapeutic efficacy of chemotherapeutic agents is limited due to the blood-brain barrier (BBB). Magnetic resonance guided focused ultrasound (MRgFUS) is a new and promising intervention for CNS tumors, which has shown success in preclinical trials. High-intensity focused ultrasound (HIFU) has the capacity to serve as a direct therapeutic agent in the form of thermoablation and mechanical destruction of the tumor. Low-intensity focused ultrasound (LIFU) has been shown to disrupt the BBB and enhance the uptake of therapeutic agents in the brain and CNS. The authors present a review of MRgFUS in the treatment of CNS tumors. This treatment method has shown promising results in preclinical trials including minimal adverse effects, increased infiltration of the therapeutic agents into the CNS, decreased tumor progression, and improved survival rates.

Trifluoromethane Destruction Mechanism in Methane-Oxygen Flame

Based on the known data on the concentrations of intermediate substances and taking into account only those elementary reactions whose kinetic parameters are known, the mechanism of CF3H destruction in a flame of methane-oxygen mixtures of various compositions is developed. It is shown that CF3H destructs in the flame in reactions with O and OH without regeneration. In rich mixture further conversion of CF3H mainly occurs due to the reactions of CF3, CF2, COF2 with atomic hydrogen, which provide inhibition of methane combustion in oxygen by trifluoromethane. In stoichiometric and especially in lean mixture a role of oxidative processes involving O and OH increases and the inhibition effect weakens. The obtained scheme qualitatively describes the entire known experimental pattern observed during the combustion of CH4/O2/CF3H mixtures.

Analysis of the Physical, Chemical and Mineralogical Properties of Gallstones in the Antalya Province of Turkey

The aim of the study is to determine chemical and physical properties of gallstones of Antalya province. In line with this, the chemical and mineralogical characteristics of 1243 gallstone samples from 69 patients were analyzed, using scanning electron microscopy (SEM) and Fourier-transform infrared spectroscopy (FTIR) analysis. &#x0D; Cholesterol, carbonate apatite, calcium carbonate, calcium phosphate carbonate, calcium bilirubinate, and protein contents were observed. Cholesterol was detected in 95% of 69 samples patients, 44 of which were obtained from female patients. X-Ray Diffractometer (XRD) analysis showed newberyite, struvite, and aragonite minerals were found in the gallstones. Newberyite was present in 59% of the samples.&#x0D; Newberyite precipitates in cases where the pH value of the patient’s bile decreases and where the amount of magnesium in the bile is high. Failure in the degradation magnesium during metabolism may lead to the formation of newberyite in gallstones. Struvite is associated with the presence of ammonium phosphate in bile. Ammonium phosphate is present in plant fertilizers and can easily be dissolved in water. These minerals (newberyite and struvite) are present in the human body and, more importantly, are intricately involved in the body’s production and destruction mechanisms. Therefore, determining the source of these minerals is crucial for understanding the mechanisms of gallstone formation.

Analysis of Soil–Water Characteristics and Stability Evolution of Rainfall-Induced Landslide: A Case of the Siwan Village Landslide

This paper aimed to study the soil–water characteristics and stability evolution law of rainfall-induced landslide. Taking the two landslide events in Siwan village as an example, the formation conditions of the disaster and landslide characteristics were analyzed. Additionally, the deformation characteristics and destruction mechanisms of landslides were discussed in-depth. The soil–water characteristics and hydraulic conductivity of the landslides were analyzed based on TRIM experiment results. Geo-Studio numerical software was further used for typical sections to analyze the stability of the evolution of the landslide events under rainfall conditions. The results showed that (1) The soil–water characteristic curve (SWCC) inversely varies with water content volume, and the sliding body has lower saturated water content and matrix suction than the sliding zone. The hydraulic conductivity function (HCF) increases with water content volume, and the sliding body has higher hydraulic conductivity (0.43 m/d) than the sliding zone (0.03 m/d). (2) Rainfall is the primary cause of landslides, and there is a hysteretic effect. Heavy rainfall will inevitably accelerate the formation of landslides in the analysis of the deformation characteristics and destruction mechanisms of rainfall-induced landslides. (3) Compared with the engineering analogy of the Fredlund and Xing (FX) model, the Van Genuchten–Mualem (VGM) model of the soil–water characteristics test based on the TRIM experimental system can better reflect the actual field situation. The numerical simulation method based on the TRIM experiments of the soil–water characteristics test is scientifically sound and reliable for the stability evolution of overburden rainfall-induced landslides.

Enhanced degradation of diazinon in aqueous solution using C-TiO2/g-C3N4 nanocomposite under visible light: Synthesis, characterization, kinetics, and mechanism studies

Sol-gel synthesis of C-TiO2/g-C3N4 nanocomposite using carboxymethyl cellulose as a carbon source and with different contents of C-TiO2 nanoparticles for the degradation of diazinon with the use of visible radiation was investigated in this study. Characteristics of the synthesized catalyst were analyzed by FE-SEM, XRD, EDX, FT-IR, BET, PL, TGA, and DRS techniques. We obtained the degradation efficiency of diazinon with C-TiO2 (30 wt%)/g-C3N4 under optimal conditions (dose of catalyst 0.1 g L−1, diazinon initial concentration 15 mg L−1, time of reaction 30 min, pH = 5.5) 98.34%, which showed premiere photocatalytic degradation efficiency compared to carbon nitride and C-TiO2. According to the data, the degradation process of diazinon by C-TiO2/g-C3N4 nanocomposite corresponds to first-order kinetics. The catalyst showed good reusability after five consecutive cycles. In addition, we investigated the effects of several scavengers on photocatalytic degradation yield and proposed the possible mechanism of diazinon destruction by C-TiO2 (30 wt%)/g-C3N4.

Water treatment by cavitation: Understanding it at a single bubble - bacterial cell level

Cavitation is a potentially useful phenomenon accompanied by extreme conditions, which is one of the reasons for its increased use in a variety of applications, such as surface cleaning, enhanced chemistry, and water treatment. Yet, we are still not able to answer many fundamental questions related to efficacy and effectiveness of cavitation treatment, such as: “Can single bubbles destroy contaminants?” and “What precisely is the mechanism behind bubble's cleaning power?”. For these reasons, the present paper addresses cavitation as a tool for eradication and removal of wall-bound bacteria at a fundamental level of a single microbubble and a bacterial cell. We present a method to study bubble-bacteria interaction on a nano- to microscale resolution in both space and time. The method allows for accurate and fast positioning of a single microbubble above the individual wall-bound bacterial cell with optical tweezers and triggering of a violent microscale cavitation event, which either results in mechanical removal or destruction of the bacterial cell. Results on E. coli bacteria show that only cells in the immediate vicinity of the microbubble are affected, and that a very high likelihood of cell detachment and cell death exists for cells located directly under the center of a bubble. Further details behind near-wall microbubble dynamics are revealed by numerical simulations, which demonstrate that a water jet resulting from a near-wall bubble implosion is the primary mechanism of wall-bound cell damage. The results suggest that peak hydrodynamic forces as high as 0.8 μN and 1.2 μN are required to achieve consistent E. coli bacterial cell detachment or death with high frequency mechanical perturbations on a nano- to microsecond time scale. Understanding of the cavitation phenomenon at a fundamental level of a single bubble will enable further optimization of novel water treatment and surface cleaning technologies to provide more efficient and chemical-free processes.

Plasma microbubbles vs gas-liquid DBD energized by low-frequency high voltage nanopulses for pollutants degradation in water: Destruction mechanisms, composition of plasma-activated water and energy assessment

The main scope of this study was the direct comparison of gas-liquid DBD and plasma microbubbles (PMB) driven by high voltage nanopulses in terms of pollutant destruction mechanisms, plasma-activated water composition and cost-effectiveness. The comparison between gas-liquid DBD and PMB was evaluated in terms of water physicochemical properties and plasma species concentrations, performance towards the degradation of dyes with different structure, energy yield and electrical energy per order (EEO) whereas the role of various critical parameters including feeding gas, gas flow rate, applied voltage, water matrix and pollutant concentration was investigated. Different plasma-liquid interactions between the two systems led to high concentrations of long-lived species (NO3–, NO2–, H2O2, O3) and a significant decrease in solution pH in gas-liquid DBD compared to low concentrations of long-lived species and near-neutral pH in PMB. PMB bears important advantages, including higher .OH concentrations, faster degradation kinetics and lower energy requirements compared to gas-liquid DBD whereas the decisive species were found to depend on the target pollutant. The energy yield of the current nanopulsed-driven PMB system was extremely high (82.1 g/kWh) whereas its EEO (0.25 kWh/m3) was one of the lower ever reported. However, the low concentrations of long-lived species and almost neutral pH may act negatively for the removal of contaminants whose degradation is favored at lower pH and/or high long-lived species concentrations. This study provides insights into both gas-liquid DBD and PMB systems contributing on the further exploitation of the plasma-based wastewater treatment.