Target Strength Measurements Research Articles

Acoustic Target Strengths and Swimbladder Morphology of Chub Mackerel Scomber japonicus in the Northwest Pacific Ocean

The Northwest Pacific chub mackerel (Scomber japonicus) is one of the most productive, economically important fishery resources worldwide. Accurately assessing this species and ensuring adherence to total allowable catch limits are crucial owing to fluctuations in their abundance and distribution. Acoustic target strength measurements of S. japonicus were conducted at 38, 70, and 120 kHz using a split-beam echosounder of individuals from nine size groups (mean fork length, 10.8–28.3 cm) swimming freely in a net cage within a seawater tank. An underwater camera was utilized to simultaneously measure swimming angle. Least-squares regression analysis revealed that when the slope was constrained to 20, as per the generally applicable morphometric equation, the resulting values for the constant term (b20) were −67.7, −66.6, and −67.3 dB at 38, 70, and 120 kHz, respectively. S. japonicus mean swimming angle across the groups was −10.5–9.6° (standard deviation [SD], 16.3–33.3°). Furthermore, the ratio of swimbladder height to swimbladder length, the ratio of swimbladder length to fork length, and the tilt angle of the swimbladder (mean ± SD) were 0.191 ± 0.060, 0.245 ± 0.055, and 9.6 ± 3.0°, respectively. These results can be used for the acoustic stock assessment of S. japonicus in the Northwest Pacific Ocean.

Investigations on Target Strength Estimation Methods: A Case Study of Chub Mackerel (Scomber japonicus) in the Northwest Pacific Ocean

Target strength (TS) is an acoustic property of individual marine organisms and a critical factor in acoustic resource assessments. However, previous studies have primarily focused on measuring TS at narrowband, typical frequencies, which cannot meet the requirements of broadband acoustic technology research. Additionally, for marine fish, conducting in situ TS measurements is challenging due to environmental constraints. Rapidly freezing and preserving fish samples for transfer to the laboratory is a common method currently used. However, the impact of freezing preservation during transportation on the swimbladder morphology and TS of swimbladder-bearing fish remains unclear. This study investigated the differences in swimbladder morphology and TS of Chub mackerel (Scomber japonicus) before and after freezing. Then, we compared different TS measurement methods through ex situ TS measurements (45–90 kHz, 160–260 kHz) and the Kirchhoff-ray mode model (KRM) simulations (1–300 kHz) and studied the broadband scattering characteristics of Chub mackerel based on the KRM model. The results showed that the morphology of the swimbladder was reduced after freezing, with significant changes in swimbladder height and volume. However, the trends of TS were not consistent and the changes were small. The difference between the KRM model and ex situ measurements was −0.38 ± 1.84 dB, indicating good applicability of the KRM. Based on the KRM results, the TS exhibited significant directivity, with fluctuations gradually decreasing and stabilizing as frequency increased. In the broadband mode, the relationship between TS and body length (L) of Chub mackerel was TS = 20log(L) − 66.76 (30 > L/λ >10). This study could provide a reference for acoustic resource estimation and species identification of Chub mackerel in the Northwest Pacific Ocean.

Target strength measurements of yellowfin tuna (Thunnus albacares) and acoustic discrimination of three tropical tuna species

Abstract Tropical tuna fisheries support some of the largest artisanal and industrial fisheries worldwide. Approximately 37% of the tropical tuna catch by industrial purse seiners is obtained from tuna associated with drifting fish aggregating devices (DFADs), where three tuna species coexist: skipjack (Katsuwonus pelamis), bigeye (Thunnus obesus), and yellowfin tuna (Thunnus albacares), with stocks subject to different health status. Purse seine fishers heavily rely on acoustic technology to detect and assess the quantity of tuna at DFADs. Yet, accurately distinguishing between species using solely acoustic methods is limited by insufficient knowledge about each species' acoustic response across frequencies. This study was carried out on six swimbladdered individuals belonging to two sets with mean lengths of 51.9 ± 9.5 and 52.9 ± 2 cm. It focuses on the frequency response obtained from ex situ measurements of yellowfin tuna recorded at 38, 70, 120, and 200 kHz, which revealed a flat response across frequencies, with b20 values of −72.4 ± 9, −73.2 ± 8, -72.3 ± 8, and −72.3 ± 9 dB, respectively. These results, contrast with previous findings on bigeye and skipjack, demonstrating the discrimination potential of acoustics in these three species. To harness this potential, a discrimination algorithm was developed.

Implementation of the split-beam function to Mills cross multibeam echo sounder for target strength measurements

Abstract Modern challenges in the increasing exploitation of aquatic ecosystems require efficient, reliable, and noninvasive technologies to acquire biomass information on a large scale. For the past 40 years, hydroacoustics has been an essential tool to analyse fish populations and their relationship with the environment. Currently, split-beam echo sounders are standard tools used to reliably and accurately record data in oceans, estuaries, and lakes. To maximize the coverage volume and to increase target detection, and therefore data quality, the use of multi-beam echo sounders is a real asset. We propose here an innovative method for target strength (TS) calculation based on the signal from a reversible Mills cross multi-beam sonar, SeapiX (Exail), which also includes the analytical capability of a split-beam echo sounder. This innovative approach provides new original information when using a multi-beam sonar. The case study in Lake Bourget was based on a comparison of the simultaneous recordings of SeapiX and EK80 (SIMRAD) to prove the validity of this multi split-beam processing, as well as to estimate the in situ TS of fish.

Experimental target strength measurements of pteropods and shrimp emphasize the importance of scattering model inputs

Abstract Shrimp and shelled pteropods are ecologically important organisms that can produce significant amounts of water column backscatter. Few studies have experimentally measured both target strength (TS) and scattering model inputs for individual shrimp and shelled pteropods, especially from the meso- and bathypelagic. We captured animals from net trawls throughout the northeast Pacific and northwest Atlantic continental shelves between 2016 and 2020. We measured morphology, density and sound speed contrasts, and broadband TS (35–75, 110–230 kHz) from tethered individuals at sea and on land in scientific aquaria. Experimentally measured TS was used to test scattering model performance. Differences in measured mean length-to-radius ratios and density contrasts of epipelagic shrimp (10–13 and 1.02–1.03) were statistically greater than for mesopelagic species (8–9 and 1.04–1.05). Differences between predicted and measured shrimp TS averaged over measurement bandwidths were <2 dB, which is similar to previous studies. Conversely, pteropod TS model predictions differed from measurements by >4 dB, with species with elongated shapes having larger differences, likely due to shell shape and uncertainty surrounding material properties. Widely used physics-based acoustic scattering models may significantly underestimate uncertainty in TS predictions for these animals.

Broadband target strength of arabesque greenling, Pacific sand lance, and pointhead flounder

Abstract Measurement of target strength (TS) is important for estimating the abundance of species using fisheries acoustics. However, most researchers have only used a limited number of representative frequencies for acoustic measurements of fish without a swim bladder (bladderless fish). Here, we measured the broadband TS of three bladderless fish species, arabesque greenling (Pleurogrammus azonus), Pacific sand lance (Ammodytes personatus), and pointhead flounder (Cleisthenes pinetorum), using two broadband echosounders. TS measurements were conducted in a seawater tank over frequency ranges of 45–90 and 80–120 kHz using a tether method. Higher TS and directivity were observed at higher frequencies than at lower frequencies for pointhead flounder and arabesque greenling. However, the TS for Pacific sand lance was relatively flat over the measured frequency spectra. Additionally, the TS of pointhead flounder and Pacific sand lance could be expressed as a function of body length and the TS of arabesque greenling could be expressed as a function of body length and frequency, which could be used in fish species discriminations and size estimations.

Target strength measurements of individual sub-Arctic krill have frequency-dependent differences from scattering model predictions.

Target strength (TS) is commonly used to convert acoustic backscatter from marine organisms to numerical abundance estimates. Shipboard, tank-based TS measurements were made on four sub-Arctic krill species (Euphausia pacifica, Thysanoessa spinifera, Thysanoessa inermis, and Thysanoessa raschii) from the eastern Bering Sea and Gulf of Alaska at discrete frequencies between 42 and 455 kHz. These measurements were compared to scattering model predictions parameterized with data from the same (when possible) individual krill. Statistically significant differences between modeled and experimental estimates at 42, 45, 120, and 131 kHz exceeded 2 dB on average. Variability in the signal-to-noise ratio, animal length, and measurements from two separate narrowband and broadband transducer pairs (at those frequencies) did not account for these differences. Scattering predictions at 120 and 131 kHz were consistent with the expected transition from Rayleigh-to-geometric scattering where models become increasingly sensitive to orientation and body shape variability. Disagreement between modeled and measured TS may be due to using scattering models developed for, and validated on, larger krill (i.e., Euphausia superba) rather than smaller species of krill. Acoustic surveys of smaller (15-30 mm) krill may require further validation of both the generalizability and parameterization of applied scattering models.

Target strength of Nile tilapia (Oreochromis niloticus) from 200 kHz calibrated fish finder and scientific echosounder: laboratory measurement and modeling

Target strength (TS) values from laboratory measurement and TS values from the model were measured and calculated from Nile tilapia (Oreochromis niloticus). Nile tilapia is one of the fastest growing aquaculture species, the fourth most produced fish globally, easily available in various sizes and lengths, and more importantly, it has a one-chambered swimbladder, which is suitable for TS measurement and modeling. The most common technique of measuring fish TS is utilizing a scientific echosounder. However, this instrument has a low implementation for small-scale fisheries due to its relatively high cost compared to the conventional fish finder. Thus, the objective of this study is to offer a solution that considers the instrument’s cost and usefulness using calibrated fish finder and scientific echosounder. TS values were produced by the two instruments that later being examined with TS from the Kirchhoff-ray mode (KRM) model to validate the result. Two single-beam echosounder instruments (Furuno FCV-628 fish finder and Simrad EK15 scientific echosounder) operated at a frequency of 200 kHz were used to assess the TS values for 30 samples of living Nile tilapia whose total length (TL) was in a range of 6 to 26 cm. In conclusion, statistically, the results showed no significant differences in TS measurement between the two instruments compared to TS values from the model. The TS values from the KRM model agreed well based on the regression analysis with the TS values from both instruments in the laboratory measurement.

Direct measurements of gas flux at seep sites using a broadband split-beam echosounder

Measurement of methane gas flux from oceanic seep sites is challenging, given the ephemeral nature and the heterogeneous spatial distribution of seeps. Acoustic systems offer a solution, as they make synoptic measurements of the water column, and the gas bubbles are strong acoustic scatterers. Here, we present a method to directly estimate gas flux using a calibrated broadband split-beam echosounder. The vertical range resolution of the broadband system facilitates discrimination of individual bubbles in the acoustic record. By comparing measurements of bubble target strength to an analytical scattering model, bubble radii can be directly measured from the acoustic data. Concurrently, split-aperture processing allows for the precise tracking of bubbles as they rise from the seafloor for measurement of bubble rise velocity. Together, the observations of bubble radius and rise velocity offer a measure of gas flux, requiring nothing more than a vessel transiting over a seep site. Application of this method to seep data collected on the East Siberian Arctic Shelf shows good agreement between resulting measurements of bubble radii (0.68–8.40 mm) with those made using optical sensors, and bubble rise velocities (4–36 cm/s) are consistent with published measurements and models. Extrapolating from single bubbles measurements, our estimates of regional methane flux (2.9 × 104 kg/year) suggest that carbon emissions in this region may be lower than previously believed.

Preliminary target strength measurement of Sprattus sprattus and its influence on biomass estimates in the Adriatic Sea (Mediterranean Sea)

Post-processing data analysis has the ability to affect target strength (TS) measurement, which in turn influence the biomass estimates of acoustic surveys. In situ experiments are considered as the best method to measure species-specific acoustic reflectivity since fish are measured in their natural environment without constrains. Nevertheless, this approach poses some problems concerning the methods to be applied to analyze acoustic data, especially when only a small number of monospecific hauls are available. In this study we tested several alternative ways to compute the b20 of sprat (Sprattus sprattus) in a poor data state in the Mediterranean Sea where the TS of the species is seldom studied. The method proposed by MacLennan and Menz (1996) and a pilot approach were used in parallel with the implementation of three single target detection algorithms to analyze two monospecific sprat hauls performed in the Adriatic Sea in 2014 and 2020. Next, we applied a Monte Carlo simulation to investigate the influence of TS variability on biomass estimates. We obtained six b20 values (range, −68.8 dB to −65.5 dB), all higher than the current known value of − 71.7 dB. The resulting coefficient of variation in the overall uncertainty on absolute biomass estimates overcame the 17%. The different models provided different total biomass estimates, however, the method proposed herein applied on high-density filter single target detection data suggest a value of b20 between − 67.5 dB and − 68.8 dB for sprat in the Mediterranean Sea, which significantly reduces absolute sprat biomass values between 2014 and 2021.

A new in-situ method to estimate fish target strength reveals high variability in broadband measurements

Fish Target Strength (TS) is a requirement for estimating abundance from surveys of pelagic fish using echosounders. This paper describes a novel in-situ method to estimate TS as a function of fish body length (L), according to the standard equation TS = 20 log (L) +b20, where b20 is the species-specific factor to be estimated. We made measurements of TS with a broadband scientific echosounder, which is becoming the new tool of choice in fisheries acoustics due to its enhanced signal-to-noise ratio, and higher range resolution and the ability to measure the frequency response. A split-beam 38 kHz broadband transducer was pole-mounted on a small boat in the fjords of Nuuk, Greenland. With the boat stationary, individual Atlantic cod Gadus morhua were detected as echotraces, caught with a fishing line, measured in length and then released. Video footage, from a camera attached to the line, was inspected to verify a single individual was attracted to the lure and caught. Fish echotrace tracking techniques were applied to estimate the tilt of the fish from each acoustic sample and the measured TS was adjusted accordingly with the use of a Kirchhoff-Approximation scattering model. The b20 parameter was estimated by linear regression. This method combines the benefits of associating TS from single fish of known length, which is typical of ex-situ methods, with the in-situ advantages of measuring TS of an undisturbed fish in its natural environment. Results yielded a b20 of − 65.6 dB ( ± 0.83 dB C.I.), which is within the range of previously published values for Atlantic cod measured with narrowband systems. Large variability of TS was observed within fish tracks (average s.d. of 5.55 dB). This is the first description of TS for Atlantic cod with broadband equipment. The high variability could not be attributed to variation in fish length nor tilt angle. Other physiological and behavioural aspects were also discarded. It is possible that this may be an intrinsic property of broadband acoustics, which indicates a need for re-evaluating TS to length relationships as fisheries science moves towards the wider application of broadband acoustics.

Estimation of Bluefin Tuna (Thunnus thynnus) mean length in sea cages by acoustical means

This paper proposes an indirect method to estimate Bluefin Tuna (Thunnus thynnus) biomass in cages using acoustic techniques. Two Simrad EK60 echosounders working at 120 and 200 kHz and a stereo camera were used to obtain target strength (TS) to fork length (FL) relationships for both operating frequencies. The equipment was placed at the bottom of a floating cage, facing towards the surface to record the ventral aspect of fish. The acoustic and optical recordings were automatically analysed and the combination of acoustic and optical results provided unequivocal TS-FL assignments. Good relationships between TS and FL were obtained for both frequencies even without discriminating data from different fish tilts and without using beam directivity compensation. Stronger correlations were obtained for compensated TS at 200 kHz when reduced tuna swimming tilt was considered. TS measurements were compared to MFS numerical predictions for a Bluefin swimbladder model, with the simulation results showing good agreement with experimental measurements. The results allow the mean tuna length to be predicted in growing or fattening cages from acoustic data raising the possibility of improving production management and of providing a useful tool for catch control estimations made by international organisations (like ICCAT) and government bodies.

Target Strength Measurements of Free-Swimming Sandeel Species, Ammodytes spp., in a Large Indoor Experimental Aquarium

The sandeel species (Ammodytes spp.) occupy a critical ecological position in connecting lower trophic levels to higher ones. However, they are strongly affected by the marine environment and their catch rates are trending downward. In this study, the target strength (TS) of sandeel species was measured in free-swimming specimens using a split-beam quantitative echo sounder with 38 and 120 kHz frequencies in a physically controlled environment. Parameters a and b used in the estimated TSmean–fork length (FL: 13.5–21.5 cm) equation were 53.7 and −124.3 dB at 38 kHz, and 71.3 and −153.2 dB at 120 kHz, respectively. The TS of the sandeel species were not proportional to the square of the FL but increased relatively rapidly with increasing body size. In addition, the mean and standard deviation of the swimming angle for the sandeel species from the acoustic data at 120 kHz were −2.2° and 7.7°, respectively, and most were in the −30° to 30°, range. Furthermore, TS was stronger at 38 kHz than at 120 kHz for all FL classes. The use of such frequency characteristics could facilitate the discrimination of fish species in the field and the sustainable assessment of sandeel species stocks.

Target strength measurement of Caroun croaker (Johnius sp.) and acoustic fish density estimation in Musi Estuary, South Sumatra

Caroun Croaker ( Johnius sp.) is one of the dominant estuarine fish that has a reasonably high economic value. The target strength (TS) value measurement is essential in calculating the density of fish stocks using the hydroacoustic method. Target strength measurement of Caroun Croaker (Johnius sp.) and an acoustic survey in Musi Estuary Waters of South Sumatra Province was conducted in December 2019. The result of TS measurement will be used to estimate the acoustic density along the survey area. The TS measurement used the standard tethered method, tying placing the fish in a fixed cage, transmitting the acoustic pulse, and analyzing its return using the scientific acoustic instrument Biosonic DT-X 200 KHz. The fisheries acoustic survey was carried out in the area around the Musi estuary with a predetermined survey design. The results of the measurements have obtained the TS-length relationship, as TS= 20 LOG L-78.79 with determinant value of R2= 0.67 for fish length ranges from 18.1-23.3 cm, and an acoustic survey showed that the estimated stock density value was 286 kg/ha for the total surveyed area of 1.612 ha.

Predation on migrating eels (Anguilla anguilla L.) from the Western Mediterranean

Nineteen female silver European eels (Anguilla anguilla L.) were tagged with satellite tags and released in the Gulf of Lion in the Mediterranean during the migration seasons 2013 and 2015. Sixteen tags transmitted data: five in the Atlantic Ocean, and eleven in the Mediterranean. Of those, 50% of migrating eels were consumed by marine mammals in each year, all in the Mediterranean. The diving behaviour recorded by the tags after the eels were consumed indicated that the most likely predators were deep diving toothed whales. Measurements of the acoustic target strength of the tag showed a negligible effect on the detectability by whale biosonar. Overall, the observed predation rate was similar to that reported for eels escaping into the Atlantic. However, unlike eels in the Atlantic, which are most vulnerable to predators in the first week of escapement as they traverse the continental shelf and before they reach the refuge of the deep ocean, eels escaping from the Mediterranean were predated in deep water, months after release, likely as a consequence of their migration within a relatively narrow and deep corridor in the Alboran Sea. This emphasises the challenge of accounting for natural mortality in management plans for the long-term recovery of the European eel.

A broadband target strength measurement method for weakly scattering animals using a 50-ms-long linear frequency modulated signal in a small tank

A broadband target strength measurement method for weakly scattering animals using a 50-ms-long linear frequency modulated signal in a small tank

In situ broadband acoustic measurements of age-0 walleye pollock and pointhead flounder in Funka Bay, Hokkaido, Japan

Measurements of the broadband acoustic backscattering from fish should improve acoustic discrimination between species. The pulse compression processing of broadband systems can be used to measure acoustic backscattering with high range resolution and improve signal-to-noise ratio. This may increase opportunities for in situ target strength (TS) measurements, the preferred method of collecting TS data. To evaluate the availability of TS spectra for acoustic discrimination, three Simrad EK80 wideband transceivers and split-beam transducers of 70, 120, and 200 kHz were used to collect in situ frequency responses of TS from age-0 juvenile walleye pollock and pointhead flounder, a swimbladderless flat-fish, distributed in and around Funka Bay, Hokkaido, Japan. The single echoes were extracted from backscattering data, and the TS spectra of the two species were obtained. However, processing of the broadband acoustic data is under discussion. To ensure the reliability of our data, the TS spectra of standard targets were also calculated. The measured TS spectra of the standard targets were close to the theoretical TS spectra. The individual TS spectra of two species included complex frequency response; however, the mean TS spectrum was relatively stable. The different characteristics of the frequency responses observed for the two species were in good agreement with reported observations. The results available for the acoustic discrimination were similar to the multi-frequency method, which uses two or more single frequencies. The raw TS spectra should aid in not only discriminating between species but also Q3 estimating the size of the fish.

First target strength measurement of Trachurus mediterraneus and Scomber colias in the Mediterranean Sea

Knowing the species-specific target strength (TS) allows converting volume backscattering strength to numerical abundance. Since the acoustic surveys conducted for biomass assessment in the Mediterranean Sea currently focus on the echoes of two or three target pelagic species, the TS of non-target species has seldom been investigated in this basin. This is the first study of the TS of two pelagic species – Mediterranean horse mackerel (Trachurus mediterraneus) and Atlantic chub mackerel (Scomber colias) – in the Mediterranean Sea. A pilot approach using tethered live fish but not involving hooks and anesthetic was tested in experiments using a split-beam scientific echosounder operating at 38, 120, and 200 kHz. The mean TS was estimated for 29 live fish. The relationship between TS and fish length was determined with a standard linear regression model; the b20 conversion parameter was obtained with the slope forced to 20. b20 was computed at all frequencies for both species. The key values at 38 kHz were −71.4 dB for T. mediterraneus and −71.6 dB for S. colias. Although these results differ from those obtained with in situ and ex situ experiments using Pacific chub mackerel and other species of the genus Trachurus, they have the potential to provide new reference values for T. mediterraneus and S. colias biomass assessment in the Mediterranean Sea. The proposed method removes some potential biases due to the unnatural behavior of anesthetized fish. Moreover, it provides an alternative to hooks, although the use of a piece of rope instead of the hook seems to increase the acoustic reflectivity of the tethering apparatus.

Comparison of mesopelagic organism abundance estimates using in situ target strength measurements and echo-counting techniques.

Recent studies using acoustic techniques suggest that the biomass of mesopelagic fishes may be an order of magnitude higher than previously estimated from trawls. However, there is uncertainty surrounding these estimates, which are derived from shipboard echosounder measurements using necessary, but poorly constrained, assumptions. Here, an echosounder is used to measure individual target strengths at depth. These measurements are used to infer mesopelagic organism density through echo-counting. Measured target strengths are used to estimate organism density by inverting shipboard echosounder measurements. The two sampling methods agree well, but highlight the importance of accurate target strength measurements.

Corrigendum to: In situ target strength measurement of the black triggerfish Melichthys niger and the ocean triggerfish Canthidermis sufflamen

Triggerfish are widely distributed in tropical waters where they play an important ecological role. The black triggerfish Melichthys niger may be the dominant species around oceanic tropical islands, whereas pelagic triggerfish, such as the ocean triggerfish Canthidermis sufflamen, can assemble around fish aggregating devices (FADs) where they are a common bycatch of tuna fisheries. In this study we combined acoustic and optical recordings to provide the first in situ target strength (TS) measurement of black and ocean triggerfish. Data were collected in the Archipelago of Fernando de Noronha off north-east Brazil. The mean TS of a 27.8-cm-long black triggerfish at 70 and 200kHz was –39.3dB re 1m2 (CV=14.0%) and –38.9dB re 1m2 (CV=14.4%) respectively. The mean TS values of ocean triggerfish (with a size range of 39–44cm) at 70 and 200kHz were –36.0dB re 1m2 (CV=15.7%) and –33.3dB re 1m2 (CV=14.0%) respectively. This work opens up the field for acoustic biomass estimates. In addition, we have shown that TS values for ocean triggerfish are within the same range as those of small tunas. Therefore, acoustic data transmitted from FADs equipped with echosounders can introduce a bias in tuna acoustic biomass estimation and lead to increased rates of bycatch.