Measurements Of Fidelity Research Articles

Precision 3D printing of chitosan-bioactive glass inks: Rheological optimization for enhanced shape fidelity in tissue engineering scaffolds

3D printing technology in tissue engineering applications provides several advantages for scaffold development, especially with natural materials, such as chitosan, which provides a biomimetic environment for cellular growth. However, chitosan hydrogel-based inks still show poor printing fidelity. In this article, we overcame this challenge by incorporating bioactive glasses (BG) nanoparticles (up to 5 wt%) into the chitosan hydrogel. The resulting inks were characterized by rheological tests, while their processability was evaluated through measurements of shape fidelity. An indirect cytotoxicity assay was also conducted to evaluate the cell viability of the printed scaffolds. The results indicated that adding BG nanoparticles to the chitosan-based ink modified its rheological properties and improved its shape-fidelity during 3D printing, which we suggest are consequences of hydrogen bonds established between the glass and the chitosan chains. Also, cytotoxicity assessment demonstrated that the resulting scaffold exhibits high cell viability. In conclusion, the proposed composite ink has optimized rheological properties for 3D printing and is promising for applications in tissue engineering.

Discrete measurements of RNA polymerase and reverse transcriptase fidelity reveal evolutionary tuning.

Direct methods for determining the fidelity of DNA polymerases are robust, with relatively little sample manipulation before sequencing. In contrast, methods for measuring RNA polymerase and reverse transcriptase fidelities are complicated by additional preparation steps that introduce ambiguity and error. Here, we describe a sequencing method, termed Roll-Seq, for simultaneously determining the individual fidelities of RNA polymerases and reverse transcriptases (RT) using Pacific Biosciences single molecule real-time sequencing. By using reverse transcriptases with high rolling-circle activity, Roll-Seq generates long concatemeric cDNA from a circular RNA template. To discern the origin of a mutation, errors are recorded and determined to occur within a single concatemer (reverse transcriptase error) or all concatemers (RNA polymerase error) over the cDNA strand. We used Roll-Seq to measure the fidelities of T7 RNA polymerases, a Group II intron-encoded RT (Induro), and two LINE RTs (Fasciolopsis buski R2-RT and human LINE-1). Substitution rates for Induro and R2-RT are the same for cDNA and second-strand synthesis while LINE-1 has 2.5-fold lower fidelity when performing second-strand synthesis. Deletion and insertion rates increase for all RTs during second-strand synthesis. In addition, we find that a structured RNA template impacts fidelity for both RNA polymerase and RT. The accuracy and precision of Roll-Seq enable this method to be applied as a complementary analysis to structural and mechanistic characterization of RNA polymerases and reverse transcriptases or as a screening method for RNAP and RT fidelity.

Assessing Residency and Site Fidelity in Bottlenose Dolphins: A Literature Review and Bibliometric Analysis

Residency and site fidelity are important parameters in population ecology for many species as they indicate the temporal and spatial use for individuals. Although both terms are clearly different, they are used interchangeably due to lack of clarity in their definitions and the ways to assess them, especially in bottlenose dolphins (Tursiops spp.). Individuals with well-defined patterns of residency and site fidelity may be more vulnerable to anthropogenic disturbances; thus, the study of these parameters may help to better assess such impacts. However, interspecific differences and logistical restrictions for the study of dolphins have prevented consensus. We set out to investigate the factors that have influenced measurements of residency and site fidelity in bottlenose and a set of other dolphin species through a retrospective (~30 y: 1990 to 2019) literature review. We found 117 scientific papers with a two-fold increasing trend approximately every 15 years, with 50% published in only four journals. Most of the studies were developed in the United States, followed by Australia and Europe, likely due to strict policies for marine mammal conservation. Author collaboration network analysis showed highly atomized national groups in which North American organizations formed the biggest cluster, followed by Australia, Africa, and Europe. However, large worldwide gaps still exist, possibly because of the heterogeneity in marine mammal sampling efforts and limited information availability in the North Pacific, Central and South America, Africa, Asia, and the Indian Ocean. We found 30 different classification methods in which 12 were based on defined categories. We provide conceptual aid to distinguish between the use of both terms, as well as guidance to tackle the challenge of defining patterns of temporal use due to the variability of thresholds for classifying individuals. A paradigm shift is needed to create comprehensive, standardized, and generalized assessments of residency and site fidelity, which can be compared based on their performance across different populations of bottlenose dolphins around the world.

High-Complexity One-Pot Golden Gate Assembly.

Golden Gate Assembly is a flexible method of DNA assembly and cloning that permits the joining of multiple fragments in a single reaction through predefined connections. The method depends on cutting DNA using a Type IIS restriction enzyme, which cuts outside its recognition site and therefore can generate overhangs of any sequence while separating the recognition site from the generated fragment. By choosing compatible fusion sites, Golden Gate permits the joining of multiple DNA fragments in a defined order in a single reaction. Conventionally, this method has been used to join five to eight fragments in a single assembly round, with yield and accuracy dropping off rapidly for more complex assemblies. Recently, we demonstrated the application of comprehensive measurements of ligation fidelity and bias data using data-optimized assembly design (DAD) to enable a high degree of assembly accuracy for very complex assemblies with the simultaneous joining of as many as 52 fragments in one reaction. Here, we describe methods for applying DAD principles and online tools to evaluate the fidelity of existing fusion site sets and assembly standards, selecting new optimal sets, and adding fusion sites to existing assemblies. We further describe the application of DAD to divide known sequences at optimal points, including designing one-pot assemblies of small genomes. Using the T7 bacteriophage genome as an example, we present a protocol that includes removal of native Type IIS sites (domestication) simultaneously with parts generation by PCR. Finally, we present recommended cycling protocols for assemblies of medium to high complexity (12-36 fragments), methods for producing high-quality parts, examples highlighting the importance of DNA purity and fragment stoichiometric balance for optimal assembly outcomes, and methods for assessing assembly success. © 2023 New England Biolabs, Inc. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Assessing the fidelity of an overhang set using the NEBridge Ligase Fidelity Viewer Basic Protocol 2: Generating a high-fidelity overhang set using the NEBridge GetSet Tool Alternate Protocol 1: Expanding an existing overhang set using the NEBridge GetSet Tool Basic Protocol 3: Dividing a genomic sequence with optimal fusion sites using the NEBridge SplitSet Tool Basic Protocol 4: One-pot Golden Gate Assembly of 12 fragments into a destination plasmid Alternate Protocol 2: One-pot Golden Gate Assembly of 24+ fragments into a destination plasmid Basic Protocol 5: One-pot Golden Gate Assembly of the T7 bacteriophage genome from 12+ parts Support Protocol 1: Generation of high-purity amplicons for assembly Support Protocol 2: Cloning assembly parts into a holding vector Support Protocol 3: Quantifying DNA concentration using a Qubit 4 fluorometer Support Protocol 4: Visualizing large assemblies via TapeStation Support Protocol 5: Validating phage genome assemblies via ONT long-read sequencing.

Tools measuring high haptic fidelity of procedural skills trainers in physician training and education: a scoping review protocol.

The objective of this scoping review is to identify instruments that measure the physical haptic fidelity of procedural skills trainers. Procedural skills trainers have demonstrated beneficial outcomes for clinicians when used to practice and rehearse procedures. Despite this, several design flaws currently limit the widespread implementation of such trainers. One notable deficit in current trainer designs is haptic fidelity. Identifying measurements of haptic fidelity may maximize the benefit of using certain training devices as well as guiding future design. This review will consider studies that assess the high fidelity haptics of procedural skills training devices in adult physicians above the level of an intern physician. Studies that do not include physicians will be excluded. The review will follow the JBI methodology for scoping reviews and will be reported in line with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR). Both published and unpublished studies will be searched for in MEDLINE (PubMed), Scopus, Web of Science Core Collection, Cochrane Central Register of Controlled Trials (CENTRAL), Embase (Ovid), CINAHL (EBSCOhost), ProQuest Dissertations and Theses (ProQuest), and Google Scholar. There will be no date, setting, or geographical limits, but only studies in English will be included. Open Science Framework osf.io/pvazu/.

Demonstration of Quantum Brachistochrones between Distant States of an Atom

Transforming an initial quantum state into a target state through the fastest possible route---a quantum brachistochrone---is a fundamental challenge for many technologies based on quantum mechanics. Here, we demonstrate fast coherent transport of an atomic wave packet over a distance of 15 times its size---a paradigmatic case of quantum processes where the target state cannot be reached through a local transformation. Our measurements of the transport fidelity reveal the existence of a minimum duration---a quantum speed limit---for the coherent splitting and recombination of matter waves. We obtain physical insight into this limit by relying on a geometric interpretation of quantum state dynamics. These results shed light upon a fundamental limit of quantum state dynamics and are expected to find relevant applications in quantum sensing and quantum computing.

Healthy Habits, Happy Homes Scotland (4HS) feasibility study: Translation of a home-based early childhood obesity prevention intervention evaluated using RE-AIM framework

ObjectiveHealthy Habits, Happy Homes (4H) is a home-based, pre-school childhood obesity prevention intervention which demonstrated efficacy in North America which we translated to Scotland (4HS) by considering contextual factors and adapting study design. RE-AIM Framework was used to assess 1) extent to which development of 4HS intervention (including recruitment) was participatory and inclusive; 2) feasibility of translating a complex public health intervention from one setting to another; 3) extent to which translation was pragmatic and 4) fidelity of intervention to the principles of Motivational Interviewing (MI). Study designFeasibility testing, process evaluation and measurements of intervention fidelity were undertaken to evaluate the translation of 4H to an economically deprived area of Scotland (4HS). Methods4HS study processes; participatory approach, recruitment methods, level of pragmatism were evaluated using the RE-AIM framework. Qualitative and quantitative measures identified key implementation features and functioning of 4HS intervention. Fidelity MI principles was determined through coding of audiotapes using Motivation Interviewing Treatment Integrity (MITI) code. ResultsKey facilitators for positive impact with families, included: inclusive recruitment methods, appropriate channels of communication and correspondence (Reach) with n ​= ​126 enquiries and n ​= ​26 (21%) families recruited. Positive links with local parents and community workers integral to the research process at n ​= ​9 meetings (Effectiveness). 61.5% of families lived in the most deprived data zone in Scotland, 23% were one parent families, thus awareness and consideration of local contextual factors (Adoption) and locally relevant materials were important. 4HS was feasible to deliver, pragmatic in nature and intervention demonstrated good fidelity to MI (Implementation). ConclusionTranslation of 4H from North America to Scotland was successful. Future studies should consider implementation of 4HS approach within routine practice within the UK (practice based evidence) or through thoughtful evaluation in a future trial (evidence based practice).

Reporter system architecture affects measurements of noncanonical amino acid incorporation efficiency and fidelity.

The ability to genetically encode noncanonical amino acids (ncAAs) within proteins supports a growing number of applications ranging from fundamental biological studies to enhancing the properties of biological therapeutics. Currently, our quantitative understanding of ncAA incorporation systems is confounded by the diverse set of characterization and analysis approaches used to quantify ncAA incorporation events. While several effective reporter systems support such measurements, it is not clear how quantitative results from different reporters relate to one another, or which details influence measurements most strongly. Here, we evaluate the quantitative performance of single-fluorescent protein reporters, dual-fluorescent protein reporters, and cell surface-displayed protein reporters of ncAA insertion in response to the TAG (amber) codon in yeast. While different reporters support varying levels of apparent readthrough efficiencies, flow cytometry-based evaluations with dual reporters yielded measurements exhibiting consistent quantitative trends and precision across all evaluated conditions. Further investigations of dual-fluorescent protein reporter architecture revealed that quantitative outputs are influenced by stop codon location and N- and C-terminal fluorescent protein identity. Both dual-fluorescent protein reporters and a "drop-in" version of yeast display support quantification of ncAA incorporation in several single-gene knockout strains, revealing strains that enhance ncAA incorporation efficiency without compromising fidelity. Our studies reveal critical details regarding reporter system performance in yeast and how to effectively deploy such reporters. These findings have substantial implications for how to engineer ncAA incorporation systems-and protein translation apparatuses-to better accommodate alternative genetic codes for expanding the chemical diversity of biosynthesized proteins.

Unbiased measurements of reconstruction fidelity of sparsely sampled magnetic resonance spectra.

The application of sparse-sampling techniques to NMR data acquisition would benefit from reliable quality measurements for reconstructed spectra. We introduce a pair of noise-normalized measurements, and , for differentiating inadequate modelling from overfitting. While and can be used jointly for methods that do not enforce exact agreement between the back-calculated time domain and the original sparse data, the cross-validation measure is applicable to all reconstruction algorithms. We show that the fidelity of reconstruction is sensitive to changes in and that model overfitting results in elevated and reduced spectral quality.

Relative Binding Free Energies of Adenine and Guanine to Damaged and Undamaged DNA in Human DNA Polymeraseη: Clues for Fidelity and Overall Efficiency.

Human DNA polymerase η (Pol η) plays an essential protective role against skin cancer caused by cyclobutane thymine–thymine dimers (TTDs), a frequent form of DNA damage arising from exposure to the sun. This enzyme rescues stalled replication forks at the TTDs by inserting bases opposite these DNA defects. Herein we calculate binding free energies for a free deoxyribose nucleotide triphosphate, dATP or dGTP, to Pol η complexed with undamaged or damaged DNA. The calculations indicate that the binding of dATP to the enzyme–DNA complex is thermodynamically favored for TTD-containing DNA over undamaged DNA, most likely because of more extensive hydrogen-bonding interactions between the TTD and the enzyme that hold the TTD more rigidly in place. The calculations also illustrate that dATP binding is thermodynamically favored over dGTP binding at both thymine positions of the TTD, most likely due to more persistent and stable hydrogen-bonding interactions between the TTD and dATP than between the TTD and dGTP. This free energy difference is slightly greater for binding at the 5′ thymine position than at the 3′ thymine position, presumably because of stabilization arising from the A:T base pair formed at the 3′ position of the TTD in the previous step of Pol η function. All of these trends in binding free energies are consistent with experimental measurements of binding strength, fidelity, processivity, and overall efficiency. The insights gained from this analysis have implications for drug design efforts aimed at modifying the binding properties of this enzyme for improving cancer chemotherapy treatments.

Parallel Processes: Using Motivational Interviewing as an Implementation Coaching Strategy

In addition to its clinical efficacy as a communication style for strengthening motivation and commitment to change, motivational interviewing (MI) has been hypothesized to be a potential tool for facilitating evidence-based practice adoption decisions. This paper reports on the rationale and content of MI-based implementation coaching Webinars that, as part of a larger active dissemination strategy, were found to be more effective than passive dissemination strategies at promoting adoption decisions among behavioral health and health providers and administrators. The Motivational Interviewing Treatment Integrity scale (MITI 3.1.1) was used to rate coaching Webinars from 17 community behavioral health organizations and 17 community health centers. The MITI coding system was found to be applicable to the coaching Webinars, and raters achieved high levels of agreement on global and behavior count measurements of fidelity to MI. Results revealed that implementation coaches maintained fidelity to the MI model, exceeding competency benchmarks for almost all measures. Findings suggest that it is feasible to implement MI as a coaching tool.

Field estimates of survival do not reflect ratings of mimetic similarity in wasp-mimicking hover flies

The evolution of mimicry, and particularly the persistence of undefended Batesian mimetic forms that are imperfect copies of their defended models, remains a central question in evolutionary biology. Previous work has demonstrated that variation in mimetic fidelity in artificial prey can alter survival. However, no studies have validated the assumption that detailed laboratory-based measurements of mimetic fidelity are actually reflected in survival in natural field experiments. Here, we demonstrate that, in line with previous studies, the mimetic similarity of 77 hover fly (Diptera: Syrphidae) species to the common wasp Vespula alascensis is strongly related to the number of abdominal stripes exhibited by the flies. We then produce three artificial pastry baits: (1) a “model” which is chemically defended and has two stripes, (2) a one-stripe mimic, and (3) an unstriped mimic. Based on the ratings study, we predicted that the one-stripe mimic would exhibit survival intermediate between the unstriped mimic and the model. Baits were deployed in experiments each involving 81 baits (27 of each kind), at 3 sites, with experiments replicated 10 times at each site for a total deployment of 2,430 baits. Proportional hazards models show that both one-striped and model baits survived equally well and significantly better than the unstriped baits, suggesting categorical prey identification rather than the use of stripe number as a continuous trait, as was suggested by the laboratory study. These findings suggest that, while humans and avian predators can distinguish mimics from models in the laboratory using a range of traits, behaviour in the field may not reflect this ability. This absence of a link between continuous measures of mimetic fidelity and prey selection may contribute to the maintenance of imperfect mimicry, but more studies using near-natural experimental paradigms are needed to investigate the phenomenon further.

A Comparison of Speech Sound Intervention Delivered by Telepractice and Side-by-Side Service Delivery Models

Telepractice has the potential to provide greater access to speech-language intervention services for children with communication impairments. Substantiation of this delivery model is necessary for telepractice to become an accepted alternative delivery model. This study investigated the progress made by school-age children with speech sound impairments in side-by-side intervention compared with telepractice intervention. Fourteen children aged 6 through 10 years with identified speech sound disorders were randomly assigned to intervention delivered in a side-by-side or telepractice service delivery model. Intervention was provided twice a week for 30-min individual sessions during a 5-week summer intervention program. Children in both service delivery models made improvement in their speech sound production during the program. There were no significant differences between the two groups on postintervention assessments including standardized assessment and listener judgments of word productions. Measurements of treatment fidelity were also reported. The results of this study support the use of telepractice in the intervention of children’s speech sound disorders.

Direct Measurements of Human Colon Crypt Stem Cell Niche Genetic Fidelity: The Role of Chance in Non-Darwinian Mutation Selection

Perfect human stem cell genetic fidelity would prevent aging and cancer. However, perfection would be difficult to achieve, and aging is universal and cancers common. A hypothesis is that because mutations are inevitable over a human lifetime, downstream mechanisms have evolved to manage the deleterious effects of beneficial and lethal mutations. In the colon, a crypt stem cell architecture reduces the number of mitotic cells at risk for mutation accumulation, and multiple niche stem cells ensure that a lethal mutation within any single stem cell does not lead to crypt death. In addition, the architecture of the colon crypt stem cell niche may harness probability or chance to randomly discard many beneficial mutations that might lead to cancer. An analysis of somatic chromosome copy number alterations (CNAs) reveals a lack of perfect fidelity in individual normal human crypts, with age-related increases and higher frequencies in ulcerative colitis, a proliferative, inflammatory disease. The age-related increase in somatic CNAs appears consistent with relatively normal replication error and cell division rates. Surprisingly, and similar to point mutations in cancer genomes, the types of crypt mutations were more consistent with random fixation rather than selection. In theory, a simple “non-Darwinian” way to nullify selection is to reduce the size of the reproducing population. Fates are more determined by chance rather than selection in very small populations, and therefore selection may be minimized within small crypt niches. The desired effect is that many beneficial mutations that might lead to cancer are randomly lost by drift rather than fixed by selection. The subdivision of the colon into multiple very small stem cell niches may trade Darwinian evolution for non-Darwinian somatic cell evolution, capitulating to aging but reducing cancer risks.

Experimental verification of clock noise transfer and components for space based gravitational wave detectors

The Laser Interferometer Space Antenna (LISA) and other space based gravitational wave detector designs require a laser communication subsystem to, among other things, transfer clock signals between spacecraft (SC) in order to cancel clock noise in post-processing. The original LISA baseline design requires frequency synthesizers to convert each SC clock into a 2 GHz signal, and electro-optic modulators (EOMs) to modulate this 2 GHz clock signal onto the laser light. Both the frequency synthesizers and the EOMs must operate with a phase fidelity of 2×10(-4)cycles/√Hz. In this paper we present measurements of the phase fidelity of frequency synthesizers and EOMs. We found that both the frequency synthesizers and the EOMs meet the requirement when tested independently and together. We also performed an electronic test of the clock noise transfer using frequency synthesizers and the University of Florida LISA Interferometry (UFLIS) phasemeter. We found that by applying a time varying fractional delay filter we could suppress the clock noise to a level below our measurement limit, which is currently determined by timing jitter and is less than an order of magnitude above the LISA requirement for phase measurements.

Rule extraction from support vector machines by genetic algorithms

Support vector machines (SVMs) are state-of-the-art tools used to address issues pertinent to classification. However, the explanation capabilities of SVMs are also their main weakness, which is why SVMs are typically regarded as incomprehensible black box models. In the present study, a rule extraction algorithm to extract the comprehensible rule from SVMs and enhance their explanation capability is proposed. The proposed algorithm seeks to use the support vectors from a training model of SVMs and combine genetic algorithms for constructing rule sets. The proposed method can not only generate rule sets from SVMs based on the mixed discrete and continuous variables but can also select important variables in the rule set simultaneously. Measurements of accuracy, sensitivity, specificity, and fidelity are utilized to compare the performance of the proposed method with direct learner algorithms and several rule-extraction techniques from SVMs. The results indicate that the proposed method performs at least as well as with the most successful direct rule learners. Finally, an actual case of pressure ulcer was studied, and the results indicated the practicality of our proposed method in real applications.

Rule extraction algorithm from support vector machines and its application to credit screening

Developing rule extraction algorithms from machine learning techniques such as artificial neural networks and support vector machines (SVMs), which are considered incomprehensible black-box models, is an important topic in current research. This study proposes a rule extraction algorithm from SVMs that uses a kernel-based clustering algorithm to integrate all support vectors and genetic algorithms into extracted rule sets. This study uses measurements of accuracy, sensitivity, specificity, coverage, fidelity and comprehensibility to evaluate the performance of the proposed method on the public credit screening data sets. Results indicate that the proposed method performs better than other rule extraction algorithms. Thus, the proposed algorithm is an essential analysis tool that can be effectively used in data mining fields.

Mechanistic insights into the roles of three linked single-stranded template binding residues of MMLV reverse transcriptase in misincorporation and mispair extension fidelity of DNA synthesis

To obtain some insights into the structure–function relationship of Moloney murine leukemia virus (MMLV) reverse transcriptase (RT), we modeled the catalytic state ternary complexes of this protein using the corresponding RT from human immunodeficiency virus type 1 (HIV-1) and available structures of MMLV RT. We observed that three MMLV RT single-stranded template binding residues, Y64, D114, and R116, act as a linked set through mutual interactions, including hydrogen bonding and ion-pairing. The analogous residues of HIV-1 RT have a somewhat different environment and they lack this linked phenomenon. To understand the functional implication of this linked set of MMLV RT, we performed site-directed mutagenesis at these three positions. Then the mutant enzymes were examined for their biochemical properties and nucleotide selectivity. Mutagenesis of these residues (Y64A, D114A, and R116A) resulted in enzymes with slight to modest changes in polymerase activities. The processivity of DNA synthesis correlated positively with the binding affinity of the MMLV RT variants. Lower fidelity in mutants was indicated by measurements of misincorporation and mispair extension fidelity of wild type (WT) and mutant RTs, in contrast to earlier works that indicate that mutations at the analogous positions in HIV-1 RT result in relatively higher fidelity. These data together with structural analysis suggest that this structural set may therefore be a key factor responsible for the different fidelity of these two RTs.

DNA Polymerase Fidelity: Comparing Direct Competition of Right and Wrong dNTP Substrates with Steady State and Pre-Steady State Kinetics

DNA polymerase fidelity is defined as the ratio of right (R) to wrong (W) nucleotide incorporations when dRTP and dWTP substrates compete at equal concentrations for primer extension at the same site in the polymerase-primer-template DNA complex. Typically, R incorporation is favored over W by 10(3)-10(5)-fold, even in the absence of 3'-exonuclease proofreading. Straightforward in principle, a direct competition fidelity measurement is difficult to perform in practice because detection of a small amount of W is masked by a large amount of R. As an alternative, enzyme kinetics measurements to evaluate k(cat)/K(m) for R and W in separate reactions are widely used to measure polymerase fidelity indirectly, based on a steady state derivation by Fersht. A systematic comparison between direct competition and kinetics has not been made until now. By separating R and W products using electrophoresis, we have successfully taken accurate fidelity measurements for directly competing R and W dNTP substrates for 9 of the 12 natural base mispairs. We compare our direct competition results with steady state and pre-steady state kinetic measurements of fidelity at the same template site, using the proofreading-deficient mutant of Klenow fragment (KF(-)) DNA polymerase. All the data are in quantitative agreement.

Nest site fidelity and clutch frequency of loggerhead turtles are better elucidated by satellite telemetry than by nocturnal tagging efforts: Implications for stock estimation

Satellite telemetry and ground-based tagging studies are complementary methods to define the spatial and temporal patterns of nesting behavior by migratory sea turtles. Estimates of site fidelity and clutch frequencies are compared for satellite telemetry versus ground truth patrols over a 6 km stretch at a southwest Florida loggerhead (Caretta caretta) rookery. Site fidelity ranged from 1.9 km to 109.1 km for all nests deposited by a female within a season. The mean site fidelity was 28.1 km for all nests, but declined to 16.9 km if omitting the first nest. Nest frequency ranged from 2 to 8 nests per season, with a modal value of 5 nests. Satellite telemetry documented a mean nest frequency of 5.4 nests per female in comparison to 2.2 nests detected by monitoring patrols. The remigrant females had higher clutch frequency, were larger in size, and had higher site fidelity compared to newly tagged females. Satellite telemetry provided improved measurements of site fidelity and reveals a need for revised fecundity estimates. If measures of clutch frequency are representative of loggerhead assemblages nesting elsewhere within the South Florida grouping, the confidence bounds on Western Atlantic loggerhead stocks are approximately 32% lower than currently accounted for annual nesting individuals.