Measurement Of Growth Hormone Levels Research Articles

Growth Hormone Status In Obese Subjects and Correlation With Age

Obesity also increases the risk of decreased Growth Hormone (GH) secretion. Decreased GH secretions have an impact on increasing body fat and decreasing lean body mass. This study aims to analyze the status of Growth Hormone (GH) in obese subjects and the correlation of GH with age. This study used a cross sectional study method using 30 obese female adolescent Body Mass Index (BMI) subjects 25-35 kg/m2, Percentage of Body Fat (PBF) above 30% and fasting blood glucose (FBG) below 100 mg/dL. Measurement of GH levels used the Enzym Link Immunosorbent Assay (ELISA) method. Data analysis techniques used the Pearson Correlation test with the Statistical Package for Social Science. Results obtained mean GH levels (761.119±504.627) pg/mL and average age (20.863±1.082) years (r =-0.483) and (p=0.023). Based on the results of the study, it can be concluded that there is a negative correlation between GH status and age in obese subjects

Measuring Growth Hormone Activity through Receptor and Binding Protein Assays

In most of the children with short stature, no organic basis can be found, which has led to the suggestion that some cases of growth failure may be due to an immunoreactive but bioinactive growth hormone (GH). In order to compare GH immunoreactivity and bioactivity (measured as receptor binding ability), a radioreceptor assay allowing the measurement of GH levels in human serum was developed using cultured human lymphocytes and 125I-labeled human GH purified by high-performance liquid chromatography. Serum samples were obtained after pharmacological stimulation with either insulin, glucagon or GRF from 19 healthy control subjects and 114 patients with various growth disturbances, aged 2.3-24.8 years. In general, there was a good correlation between the GH levels measured by the two methods, the RRA-GH levels being lower than the RIA-GH levels at all times irrespective of the stimulation test. In all the groups studied, most of the individual RRA/RIA ratios were within normal limits. It is concluded that the presence of an abnormal (bioinactive) GH molecule is extremely rare in patients with short stature.