This study investigates the fracture behavior of additively manufactured round-tip V-notched diagonally loaded square plate (RV-DLSP) specimens with different notch opening angles and tip radii produced from the Polylactic acid (PLA) with [0/90/45/-45]s raster orientation using the fused deposition modeling (FDM) technique. PLA is known for its ductile behavior, making it a suitable material for various engineering applications. Also, the layer-wise manufacturing process in FDM technique makes the PLA specimens be actually anisotropic. To take the nonlinearity and anisotropy of PLA simultaneously into consideration in prediction of the fracture loads of RV-DLSP specimens, the present study employs the Virtual Isotropic Material Concept (VIMC) and modified Equivalent Material Concept (MEMC) in a two-level strategy of simplifying PLA to an isotropic linear elastic virtual material. Then, the mean stress (MS) and maximum tangential stress (MTS) criteria are utilized to estimate the fracture loads. The theoretical estimations reveal that the VIMC-MEMC-RV-MS criterion is the most accurate model, demonstrating its effectiveness in predicting the fracture behavior of RV-DLSP specimens. Additionally, the VIMC-MEMC-RV-MTS criterion shows commendable accuracy, particularly for the specimens with 30 (deg.) notch opening angle. The scanning electron microscopy (SEM) analysis of the fracture surfaces provides further insights into the fracture mechanisms of RV-DLSP specimens. Notably, distinct fracture patterns are observed based on variations in the notch geometry. Specimens with smaller notch tip radii exhibit fiber cleavage, while those with larger radii display greater fiber interpenetration. These SEM observations are consistent with the fracture load data, which indicates higher fracture loads with increasing the notch opening angle and tip radius. By integrating VIMC and MEMC with the two fracture criteria, accurate predictions of the notch fracture toughness can be achieved, facilitating the design and optimization of 3D-printed PLA components against fracture.