Mean Permeability Coefficient Research Articles

Next generation antibiotic combinations to combat pan-drug resistant Klebsiella pneumoniae

Antimicrobial resistance has emerged as one of the leading public health threats of the twenty-first century. Gram-negative pathogens have been a major contributor to the declining efficacy of antibiotics through both acquired resistance and tolerance. In this study, a pan-drug resistant (PDR), NDM-1 and CTX-M-15 co-producing isolate of K. pneumoniae, CDC Nevada, (Kp Nevada) was exposed to the clinical combination of aztreonam + ceftazidime/avibactam (ATM/CAZ/AVI) to overcome metallo-β-lactamases. Unexpectedly, the β-lactam combination resulted in long filamentous cell formation induced by PBP3 inhibition over 168 h in the hollow fiber infection model experiments with eventual reversion of the total population upon drug removal. However, the addition of imipenem to the two drug β-lactam combination was highly synergistic with suppression of all drug resistant subpopulations over 5 days. Scanning electron microscopy and fluorescence microscopy for all imipenem combinations in time kill studies suggested a role for imipenem in suppression of long filamentous persisters, via the formation of metabolically active spheroplasts. To complement the imaging studies, salient transcriptomic changes were quantified using RT-PCR and novel cassette assay evaluated β-lactam permeability. This showed significant upregulation of both spheroplast protein Y (SPY), a periplasmic chaperone protein that has been shown to be related to spheroplast formation, and penicillin binding proteins (PBP1, PBP2, PBP3) for all combinations involving imipenem. However, with aztreonam alone, pbp1, pbp3 and spy remained unchanged while pbp2 levels were downregulated by > 25%. Imipenem displayed 207-fold higher permeability as compared with aztreonam (mean permeability coefficient of 17,200 nm/s). Although the clinical combination of aztreonam/avibactam and ceftazidime has been proposed as an important treatment of MBL Gram-negatives, we report the first occurrence of long filamentous persister formation. To our knowledge, this is the first study that defines novel β-lactam combinations involving imipenem via maximal suppression of filamentous persisters to combat PDR CDC Nevada K. pneumoniae.

Effects of natural antioxidants on the oxidative stability of Eucommia ulmoides seed oil: Experimental and molecular simulation investigations

Eucommia ulmoides seed oil with high health potential is prone to oxidative rancidity due to its rich unsaturated fatty acids. In this work, three natural antioxidants were selected for exploring the oxidation resistance of the oil compared with the common synthetic antioxidant BHT. Antioxidant activity and its dispersion and migration as well as oxygen barrier performance were predicted via the bond dissociation enthalpy (BDE), mean square displacement (MSD), binding energy (Ebinding) and permeability coefficient (S). The predicted comprehensive performance is as follows: myricetin > epicatechin > caffeic acid > BHT. Free radical scavenging assay and Rancimat assay confirmed the antioxidant activity and protective effect on oil. That is the protective effect of three natural antioxidants on Eucommia ulmoides seed oil is better than BHT and myricetin shows the optimal comprehensive performance. The induction period of myricetin/lipid system increased 164.5% compared with the control. The experimental results are in good consistent with the simulation predictions.

Analysis of the Thermal Behavior of Polypropylene-Camphor Mixtures for Understanding the Pathways to Polymeric Membranes via Thermally Induced Phase Separation.

An experimental phase diagram of the isotactic polypropylene-camphor system is constructed using an original optical method. It considerably deviates from the dynamic diagram, which can be obtained using conventional differential scanning calorimetry (DSC), and contains an additional boundary line that describes camphor solubility in the polymer. An accurate phase diagram makes it possible to perform a detailed and consistent thermodynamic analysis of the DSC, optical, and scanning electron microscopy data on the cooling of prehomogenized mixtures of different compositions, which leads to the formation of capillary-porous bodies via thermally induced phase separation. The removal of camphor results in the formation of polypropylene membranes, the morphology and functional properties of which, such as the total pore volume, mean pore size, permeability coefficient, and breaking stress, appear to be highly dependent on the composition of the initial binary system. It is shown that thermally induced phase separation induces the formation of microscopic cracks in the studied membranes. The crack density decreases with the polymer content in the initial system, but at 53 wt % of polypropylene, the membrane becomes completely impermeable to isopropanol despite the presence of large ∼4 μm pores, thus questioning the perspectives of its practical use. In general, the study makes it possible to achieve a deeper understanding of the membrane formation process via thermally induced phase separation in the mixtures of semicrystalline polymers with low molar mass substances.

Validation of an immortalized human (hBMEC) in vitro blood-brain barrier model.

We recently established and optimized an immortalized human in vitro blood-brain barrier (BBB) model based on the hBMEC cell line. In the present work, we validated this mono-culture 24-well model with a representative series of drug substances which are known to cross or not to cross the BBB. For each individual compound, a quantitative UHPLC-MS/MS method in Ringer HEPES buffer was developed and validated according to current regulatory guidelines, with respect to selectivity, precision, and reliability. Various biological and analytical challenges were met during method validation, highlighting the importance of careful method development. The positive controls antipyrine, caffeine, diazepam, and propranolol showed mean endothelial permeability coefficients (P e) in the range of 17-70 × 10(-6) cm/s, indicating moderate to high BBB permeability when compared to the barrier integrity marker sodium fluorescein (mean P e 3-5 × 10(-6) cm/s). The negative controls atenolol, cimetidine, and vinblastine showed mean P e values < 10 × 10(-6) cm/s, suggesting low permeability. In silico calculations were in agreement with in vitro data. With the exception of quinidine (P-glycoprotein inhibitor and substrate), BBB permeability of all control compounds was correctly predicted by this new, easy, and fast to set up human in vitro BBB model. Addition of retinoic acid and puromycin did not increase transendothelial electrical resistance (TEER) values of the BBB model.

High gastrointestinal permeability and local metabolism of naringenin: influence of antibiotic treatment on absorption and metabolism.

The present study aims to determine the permeability of naringenin in the stomach, small intestine and colon, to evaluate intestinal and hepatic first-pass metabolism, and to study the influence of the microbiota on the absorption and disposition of naringenin (3.5 μg/ml). A single-pass intestinal perfusion model in mice (n 4-6) was used. Perfusate (every 10 min), blood (at 60 min) and bile samples were taken and analysed to evaluate the presence of naringenin and its metabolites by an HPLC-MS/MS method. To study the influence of the microbiota on the bioavailability of naringenin, a group of animals received the antibiotic rifaximin (50 mg/kg per d) for 5 d, and naringenin permeability was determined in the colon. Naringenin was absorbed well throughout the gastrointestinal tract but mainly in the small intestine and colon (mean permeability coefficient 7.80 (SD 1.54) × 10(-4) cm/s and 5.49 (SD 1.86) × 10(-4) cm/s, respectively), at a level similar to the highly permeable compound, naproxen (6.39 (SD 1.23) × 10(-4) cm/s). According to the high amounts of metabolites found in the perfusate compared to the bile and plasma, naringenin underwent extensive intestinal first-pass metabolism, and the main metabolites excreted were sulfates (84.00 (SD 12.14)%), followed by glucuronides (8.40 (SD 5.67)%). Phase II metabolites were found in all perfusates from 5 min of sampling. Mice treated with rifaximin showed a decrease in naringenin permeability and in the amounts of 4-hydroxyhippuric acid and hippuric acid in the lumen. Naringenin was well absorbed throughout the gastrointestinal tract and its poor bioavailability was due mainly to high intestinal metabolism.

Evaluation of ultrasound and glucose synergy effect on the optical clearing and light penetration for human colon tissue using SD‐OCT

Topical application optical clearing agents (OCAs) can effectively enhance the tissue optical clearing on the human colon tissue, which has been demonstrated in our previous studies. Nevertheless, the strong light scattering still limits the diffusion rate of OCAs and penetration depth of light into the tissue. In this study, in order to further increase the diffusion of the OCA of glucose into tissue, we employ a method to improve the glucose permeability and light penetration with ultrasound (sonophoretic delivery, SP) and glucose (G) synergy on human normal and cancerous colon tissues in vitro, which was measured and quantified with spectral-domain optical coherence tomography (SD-OCT) technology. To evaluate the effect of ultrasound mediation, the percentages of OCT signal enhancement (PSE) and 1/e light-penetration depth were calculated for G alone and ultrasound-G treatments. The PSE was calculated at approximately 313 μm from the sample tissue surface. For normal and cancerous colon tissues the PSE were about 91.1 ± 10.6% and 65.3% ± 12.3% with 30% G/SP, but for the 30% G alone treatment it was about 78.6 ± 11.2% and 54.5% ± 9.3%, respectively. The max value of 1/e light-penetration depth for normal colon tissue was 0.47 ± 0.02 mm with 30% G alone and 0.60 ± 0.05 mm (p < 0.05)with 30% G/SP synergy. However, for the cancerous colon tissue the max value was 0.45 ± 0.01 mm and 0.57 ± 0.03 mm (p < 0.05), respectively. The obtained permeability coefficients showed a significant enhancement with ultrasound mediation. The mean permeability coefficients of 30% G/SP in normal and cancerous colon tissues were (6.3 ± 0.16) × 10(-6) cm/s and (12.1 ± 0.34) × 10(-6) cm/s (p < 0.05), respectively. These preliminary experiments showed that ultrasound can effectively enhance the tissue optical clearing and glucose diffusion rate as well as increase the light-penetration depth into biotissues.

A Statistical Evaluation of Consolidation Properties of Marine Clay in South Korea

In this study consolidation properties of marine clay in south Korea were investigated through standard consolidation tests (SCT) and constant rates of strain (CRS) tests, and Rowe Cell tests. This examination was conducted based on the field data collected in Kwangyang Bay area, which is one of the representative harbor regions in the southern coast of Korea. Consolidation parameters, including preconsolidation pressure (P c ), compression index (C c ), swelling index (C s ), overconsolidation ratio (OCR), vertical and horizontal consolidation coefficient (C v , C h ), vertical and horizontal coefficients of permeability (K v , K h ), were obtained through the statistical analysis. A correlation between these parameters was evaluated by the regression analysis after removing outliers of the collected data. As a result, it was shown that the relationship between swelling index and compression index was C s = 0.0969C c and between modified compression index and compression index was C c ′ = 1.1411C c(lab). Furthermore, the mean consolidation coefficient ratio (C h /C v ) and permeability coefficient ratio (K h /K v ) were found to be 2.34 and 2.21, respectively.

Assessment of the effects of ultrasound-mediated glucose on permeability of normal, benign, and cancerous human lung tissues with the Fourier-domain optical coherence tomography

The objective of this study was to evaluate the effects of ultrasound-mediated analyte diffusion on permeability of normal, benign, and cancerous human lung tissue in vitro and to find more effective sonophoretic (SP) delivery in combination with the optical clearing agents (OCAs) method to distinguish normal and diseased lung tissues. The permeability coefficients of SP in combination with OCAs diffusion in lung tissue were measured with Fourier-domain optical coherence tomography (FD-OCT). 30% glucose and SP with a frequency of 1 MHz and an intensity of 0.80 W/cm2 over a 3 cm probe was simultaneously applied for 15 min. Experimental results show that the mean permeability coefficients of 30% glucose/SP were found to be (2.01±0.21)×10(-5) cm/s from normal lung (NL) tissue, (2.75±0.28)×10(-5) cm/s from lung benign granulomatosis (LBG) tissue, (4.53±0.49)×10(-5) cm/s from lung adenocarcinoma tumor (LAT) tissue, and (5.81±0.62)×10(-5) cm/s from lung squamous cell carcinoma (LSCC) tissue, respectively. The permeability coefficients of 30% glucose/SP increase approximately 36.8%, 125.4%, and 189.1% for the LBG, LAT, and LSCC tissue compared with that for the NL tissue, respectively. There were statistically significant differences in permeability coefficients of 30% glucose/SP between LBG and NL tissue (p<0.05), between LAT and NL tissue (p<0.05), and between LSCC and NL tissue (p<0.05). The results suggest that the OCT functional imaging technique to combine an ultrasound-OCAs combination method could become a powerful tool in early diagnosis and monitoring of changed microstructure of pathologic human lung tissue.

Cross-Linking with Ultraviolet-A and Riboflavin Reduces Corneal Permeability

To investigate the effect of cross-linking treatment on corneal permeability in a live animal model. Rabbit eyes were selected at random to be left unoperated or to undergo epithelial debridement with or without treatment consisting of cross-linking (CXL) with riboflavin and ultraviolet-A. Nine eyes received a total dose of 3.6 J/cm² and after epithelial healing the corneas were placed in a two-chamber system for quantification of the diffusion of fluorescein compared with controls. Thirty eyes received a total dose of 5.4 J/cm² and, after epithelial healing, in vivo corneal permeability was quantified as the pupillary response over a 30-minute period to a dose of topical pilocarpine compared with controls. In the ex vivo assay, the mean permeability coefficient in the CXL group (2.42 × 10⁻⁷) was reduced when compared with the unoperated controls (3.73 × 10⁻⁷; P = 0.007) and to the eyes that received epithelial debridement alone (3.74 × 10⁻⁷; P = 0.01). In the in vivo permeability assay, the change in pupillary diameter at 30 minutes after pilocarpine administration was smaller in the CXL group (-1.9 mm), compared with the epithelial debridement group (-2.6 mm; P < 0.001) and with the unoperated controls (-2.7 mm; P = 0.003). Corneal cross-linking with ultraviolet-A and riboflavin results in a statistically significant reduction in corneal permeability. These findings suggest that dosing of topical medications may need to be increased in eyes with a history of CXL to achieve expected therapeutic effects, and they may have implications for the long-term health of the cornea.

Penetration Enhancing Effect of Polysorbate 20 and 80 on the &lt;i&gt;In Vitro&lt;/i&gt; Percutaneous Absorption of LAscorbic Acid

Purpose: To investigate the penetration enhancing effect of two polysorbates - polyoxyethylene 20 (POE-20) and polyoxyethylene 80 (POE-80) - on the in vitro percutaneous absorption of ascorbic acid (AA). Methods: For the permeation experiments, Franz diffusion cell covered with aluminum foil providing an effective diffusion area of 1.76 cm 2 and hairless rabbit skin were used. A range of concentrations (1 – 5 %) of POE-20 and POE-80 was added to the ascorbic acid to determine their optimum enhancement concentration. Results: The cumulative amount of AA that diffused across the skin increased with increase in the concentration of the permeation enhancers. Without the enhancer, AA flux was 0.626 µg/cm 2 /h while mean permeability coefficient (K p) was 2.09 × 10 -6 cm/h. AA flux was 3.17 and 2.44 µg/cm 2 /h for POE-20 and POE-80, respectively, while mean permeability coefficient was 10.6 × 10 -6 and 8.14 × 10 -6 K p, cm/h. Maximum flux (3.16 µg/cm 2 /h) at POE-20 concentration of 5 % was obtained, with an enhancement ratio (ER) of 5.07 in relation to control (i.e., AA without enhancer). For POE-80 (5 %), maximum flux was 2.44 µg/cm 2 /h with an ER value of 3.89, compared to control. Conclusion: This study demonstrates that POE-20 and POE-80 exerted a penetration enhancing effect on the percutaneous absorption of L-ascorbic acid (AA).

Effects of freezing on human skin permeability.

The percutaneous absorption of water was measured in-vitro at 30 degrees C for pale caucasian abdominal skin which had been stored at -20 degrees C for up to 466 days and compared with fresh skin. Prolonged freezing of the skin did not affect the absorption of water which had a mean permeability coefficient of 1.71 +/- 0.62 X 10(-3) cm h-1 (180 diffusion experiments with 39 skin specimens). No significant difference was found between the absorption of water through human skin which was fresh or had been frozen. The mean permeability coefficient for skin which had not been frozen was 1.30 +/- 0.55 X 10(-3) cm h-1 for 6 skin specimens.

Effect of surfactants and pH on naltrexone (NTX) permeation across buccal mucosa

The objective of this pre-formulation study was to systematically investigate the effects of two surfactants (Brij 58 ® and Tween 80 ®) and change in solution pH on in vitro permeation of naltrexone HCl (NTX-HCl) across tissue engineered human buccal mucosa. For the study, 10 mg/ml solutions of Tween 80 ® (0.1 and 1%, w/v) and Brij 58 ® (1%, w/v) were prepared in standard artificial saliva buffer solution (pH 6.8). For studying pH effects, solution pH was adjusted to either 7.5 or 8.2. As controls, three concentrations of NTX-HCl (2.5, 10 and 25 mg/ml) were prepared. Using NTX standard solution (10 mg/ml; pH 6.8), the permeation was observed between in vitro human and ex vivo porcine mucosa. It was observed that Brij 58 ® increased the permeation rates of NTX significantly. The flux of 10 mg/ml solution (pH 6.8) increased from 1.9 ± 0.6 (×10 2) to 13.9 ± 2.2 (×10 2) μg/(cm 2 h) (approximately 6-fold) in presence of 1% Brij 58 ®. Increasing pH of NTX-HCl solution was found to increase the drug flux from 1.9 ± 0.6 (×10 2) (pH 6.8) to 3.0 ± 0.6 (×10 2) (pH 7.4) and 8.0 ± 3.5 (×10 2) (pH 8.2) μg/(cm 2 h), respectively. Histological analyses exhibited no tissue damage due to exposure of buccal tissue to Brij 58 ®. The mean permeability coefficients ( K p ) for 2.5, 10 and 25 mg/ml solutions of NTX-HCl (pH 6.8) were 5.0 (×10 −2), 1.8 (×10 −2) and 3.2 (×10 −2) cm/h, respectively, consistent with data from published literature sources. Increase of NTX flux observed with 1% Brij 58 ® solution may be due to the effects of ATP. Increase in flux and the shortening of lag time observed by increasing in solution pH confirmed earlier finding that distribution coefficient (log D) of NTX is significantly affected by small increments in pH value and therefore plays an important role in NTX permeation by allowing faster diffusion across tissue engineered human buccal tissue.

Ion mobility spectrometry as a high-throughput technique for in vitro transdermal Franz diffusion cell experiments of ibuprofen

Rapid, low-cost and sensitive analytical methods are needed to analyse the large number of samples that are generated when investigating the absorption profile of drugs through the skin using Franz diffusion cell experiments (FDC). The goal of this study was to evaluate the potential of ion mobility spectrometry (IMS) for the quantitative analysis of active pharmaceutical ingredients (API) in transdermal research. Ibuprofen was used as a model drug and the optimal IMS parameters were determined using a Doehlert experimental design. To assess the usefulness of the IMS method, FDC experiments using human skin were conducted, covering a concentration range of 0.32–69.57 μg/ml. The resulting analytical samples were analysed using IMS and subsequently compared to HPLC as a reference method. No significant differences were found between the results obtained using both analytical methods, with a mean skin permeability coefficient ( K p) value of 0.013 cm/h. The combination of fast detection times, sensitivity, low costs and easy maintenance of IMS instruments makes this technique an attractive alternative for HPLC in this type of experiments.

A simple and efficient approach for calculating permeability coefficients and HETP for rectangular columns

There had been little progress in development of the theoretical basis of rectangular chromatography columns until Spangler made great progress by using a more exact model than Golay's. Unfortunately, there was a deficiency in his calculations, which led to a conclusion inconsistent with the previous theories. In this paper, a simpler formula with defined variables was first established to calculate the mean permeability coefficient for a rectangular GC column. A formula was also established to calculate the height equivalent to a theoretical plate (HETP) for a rectangular column based on this work and the correction of Spangler's theory. By comparing both our predictions and Spangler's predictions with Golay's, respectively, we could demonstrate that our theory is more exact. Further, one parameter (A) was found to be not monotonous. This finding leads to the conclusion that the square column has the highest performance among all the rectangular-shaped columns used for chromatography, and that a width/depth ratio of around three is desirable if the column is used for mixing reactants in lab-on-a-chip systems, instead of for chromatography. The conclusions are applicable not only for gas but also for liquid chromatography columns.

Sorption and permeation behavior for CO2 in NH3-plasma-treated and untreated polystyrene membranes

In our preceding work, the simultaneous deviation from the conventional dual-mode sorption and mobility model was observed in a case of CO2 in polystyrene (PS) membrane (glass transition temperature of pure polymer = 95°C) at 60 and 70°C. The plasticization effect of sorbed CO2 on both the sorption and diffusion processes tends to be brought about in glassy polymer membranes near the glass transition temperature. The behavior was simulated based on the concept that only one population of sorbed gas molecules of plasticizing ability to the polymer should exist. In the present work, the sorption and permeation behavior for CO2 in NH3-plasma-treated and untreated PS membranes at 40°C was investigated, where NH3-plasma treatment was executed at plasma discharge powers of 40, 80, 120, and 160 W for an exposure time of 2 min. The sorption isotherm could be described by the sorption theory of Mi et al. (Macromolecules 1991, 24, 2361), where the glass transition temperature is depressed by a concentration of sorbed CO2 of plasticizing ability. NH3-plasma treatment on PS membrane had little influence on the sorption behavior of CO2 at plasma discharge powers up to 160 W. The mean permeability coefficients were somewhat increased only at a discharge power of 160 W. At CO2 pressures below 0.9 MPa, the pressure dependencies of mean permeability coefficients for CO2 in both NH3-plasma-treated and untreated PS membranes at 40°C could also be simulated on the basis of the concept that only one population of sorbed gas molecules of plasticizing ability should exist. At CO2 pressures above 0.9 MPa, however, a plasticization action of sorbed CO2 had much more influence on the diffusion process rather than on the sorption one, and such a combined concept underestimated the mean permeability coefficient. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 103: 1798–1805, 2007

Gas permeabilities of NH3‐plasma‐treated polyethersulfone membranes

AbstractThe effect of NH3‐plasma treatment on glassy polyethersulfone (PES) membranes upon the diffusion process for CO2, O2, and N2. was investigated from the permeability measurements. The permeation behavior for O2 and CO2 in untreated and NH3‐plasma‐treated PES membranes was simulated well in terms of the dual‐mode mobility model. For O2 transport, NH3‐plasma treatment on PES membrane had a little influence on the diffusion process of Langmuir species and very little influence on the diffusion process of Henry's law species. For CO2 transport, it promoted the transport of Henry's law mode but had very little influence on the transport process of Langmuir species. Both the mean permeability coefficient to CO2 and the ideal separation factor for CO2 relative to N2 took maximum values at a treatment power of 40 W. © 2004 Wiley Periodicals, Inc. J Appl Polym Sci 94: 758–762, 2004

Coarse-grained nonequilibrium approach to the molecular modeling of permeation through microporous membranes.

We present a modeling technique that combines a statistical-mechanical coarse-graining scheme with a nonequilibrium molecular simulation algorithm to provide an efficient simulation of steady-state permeation across a microporous material. The coarse-graining scheme is based on the mapping of an atomistic model to a lattice using multidimensional free-energy and transition-state calculations. The nonequilibrium simulation algorithm is a stochastic, lattice version of the recently developed atomistic dual-control-volume grand canonical molecular dynamics. We demonstrate the approach on a model of methane permeating through a bulk portion of siliceous zeolite ZK4 at 300 K under imposed fugacity differences. We predict the coarse-grained (cage-level) density profiles and observe the development of nonlinearities as the magnitude of the fugacity difference is increased. From the net flux of methane we also predict a mean permeability coefficient under the various conditions. The simulation results are obtained over time scales on the order of microseconds and length scales on the order of dozens of nanometers.

Gas permeability of NH3‐plasma‐treated poly(methyl methacrylate) membranes

AbstractThe effect of NH3 plasma treatment on glassy poly(methyl methacrylate) (PMMA) membranes on the diffusion process for penetrant gases (CO2, O2, and N2) was investigated from mean permeability data. The mean permeability coefficient for CO2 definitely depended on the upstream pressure, whereas those for O2 and N2 remained constant regardless of the upstream pressure. For O2 transport, the permeability increased a little with increasing treatment power, and for N2 transport, it was not affected by the treatment power. For CO2 transport, NH3 plasma treatment promoted the transport of Langmuir mode, presumably through an increased Langmuir capacity constant for CO2. NH3 plasma treatment for PMMA membranes resulted in an increase in the separation factor of CO2 relative to N2 and in the permeability to CO2. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 87: 1068–1072, 2003

Quantitative Outcrop Data for Flow Simulation

ABSTRACT An architectural analysis documents variations in bedding geometry and rock properties within a tide-influenced deltaic sandstone exposed in the Cretaceous Frontier Formation of central Wyoming, USA. Digital maps of bedding, lithofacies, and diagenetic cements, as well as vertical logs of grain size, lithofacies, and permeability, describe rock properties that potentially influence fluid flow behavior. These records are used to construct simulation models that assess the relative importance of different types of geologic variability on prediction of subsurface fluid flow. Two 25-meter-thick tide-influenced deltaic sandstone bodies coarsen upward and contain inclined beds that reflect episodic delta-front progradation. Decimeters- to meters-thick beds within bodies alternate between cross-stratified sandstones formed during rapid flows and shales deposited during more quiescent conditions. Down depositional dip, bed-draping shales are more continuous and lithofacies within sandstone beds become finer-grained and increasingly heterolithic. As sandstone beds fine down dip, mean permeability values decrease and coefficients of variation increase, permeability values change from nearly normal distributions to highly right-skewed, and permeability values become more strongly spatially correlated. Nodular cements also affect permeability. All of these variations were modeled using stratigraphic cornerpoint grids that preserve stratal geometry and gridblocks with properties assigned using a combination of rock property maps and statistical models based on rock property logs. Simulations predict effects on fluid flow of geologic heterogeneity at different scales, the influences of process variables, and the effects of different methods of grid construction and rock property assignment. Flow simulations of water flooding through a 22 m thick by 360 m long segment of a deltaic sandstone oil reservoir predict that: (1) rapid flow through coarser-grained deposits at the top of the sandstone body tends to draw water upward; (2) thin shales draping sandstone beds shunt downdip-directed flow downward and updip-directed flow upward; (3) cement nodules cause more tortuous flow patterns but have little effect on recovery efficiency; (4) Methods of predicting intrafacies correlation of permeability have little effect on flow behavior at this scale. A simulation model constructed using a high-resolution Cartesian grid did not resolve the effects of inclined shales, demonstrating the usefulness of stratigraphic cornerpoint grids for modeling flow through complex geologic deposits. Flow simulations of tracer flow through a meter-thick cross-stratified bed within the deltaic sandstone body showed that at this scale shale drapes and models of the intrafacies distribution of permeability have statistically significant effects.

Diffusion of water through various polymer films: a new high performance method of characterization

Diffusion of water in polymer films has been characterized by using a new high performance and reproducible method based on permeation measurements. With this method, which uses a fast response water-specific sensor based on dew point measurement, it is possible to determine, from the transient step, the time lag water diffusion coefficient DL and, when the steady state is reached, a mean water permeability coefficient P. In this work we have emphasized the water concentration dependence of diffusion coefficient D. After calibration, the technique yields accurate values of P, consistent with literature data. By testing various polymers, different behaviours with respect to water have been observed, particularly with low density polyethylene (LDPE) which shows significant hydrophobic properties.