In tropical Africa, allergies are not well documented. The objective of this work was to evaluate, by two methods, the sensitization to mites in children followed for respiratory allergy. Skin prick-test and IgE assay by REAST test with 3 mites: Dermatophagoides pteronyssinus (D.pteronyssinus), Dermatophagoides farinae (D.farinae) and Blomia tropicalis (B.tropicalis) were carried out in children from 3 to 15 years followed up for asthma and/or allergic rhinitis. The positive results of the two tests were compared. Of the 130 (100%) children included, all eligible for the assay, 119 (91.5%) had the prick-test. The mean age and sex ratio (M/F) were 7±1 year, and 1.6. The association of rhinitis and asthma was the most frequent and found in 66 (55.6%). The sensitivity frequencies for the prick-test and assay were respectively 79% versus 36.1% for B.tropicalis, 71.4% versus 33.4% for D.pteronyssinus and 38.7% versus 37.8% for D.farinae. A moderate correlation between mean papule diameter and mean IgE concentration was observed. In African tropical environments, dust mite sensitization in children followed for respiratory allergy is frequent, with the order of frequency being: B.tropicalis, D.pteronyssinus, and D.farinae. The prick-test had better sensitivity than the assay for its evaluation.