Mean Corpuscular Hemoglobin Research Articles

The effects of dietary Agaricus bisporus powder on growth performance, haematological indices, and serum immune response in beluga (Huso huso) juvenile

IntroductionThe present study investigates the effects of mushroom powder (MP) on growth parameters, haematological indices, innate immune response, and serum biochemical indices in beluga (Huso huso) juveniles.MethodsA total of 120 fish (45 ± 0.5 g) were stocked with 10 fish in each 300-L tank. Experimental diets were prepared by inclusion of 0, 0.5, 1.0, and 2.0% MP. At the end of the feeding trial haemato-immunological parameters as well growth performance were measured.ResultsThe growth parameters results revealed that body weight, specific growth rate (SGR), and body length increased significantly in the fish fed diets containing 1% and 2% MP (P<0.05). Haematological indices results indicated that none of the experimental diets showed significant effects on red blood cell count (RBC), hemoglobin, mean corpuscular volume (MCV), mean cell hemoglobin (MCH), and mean corpuscular hemoglobin concentration (MCHC) (P >0.05). However, the haematocrit increased significantly in the fish fed diets containing 1% MP compared to the control (P<0.05). White blood cell count (WBC) in the fish fed 2% MP was significantly higher than that in the fish fed 0% and 0.5% MP (P<0.05). Furthermore, MP treatments caused no significant change in the activity of complement C3 (P >0.05), while C4 activity increased significantly in the fish fed 2% MP (P<0.05). Total immunoglobulin in 1% MP had no significant difference when compared with control (P >0.05). Albumin level was significantly higher in fish fed 2% MP compared to control (P<0.05).DiscussionIn conclusion, MP (2%) can be considered to improve growth parameters and immune indices in beluga juveniles.

HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS AS PROGNOSTIC INDICATORS FOR SUCCESSFUL REHABILITATION IN CONFISCATED TEMMINCK'S PANGOLINS (SMUTSIA TEMMINCKII).

The establishment of prognostic indicators in wildlife patients aids clinical decision-making and resource allocation. Hematological and plasma biochemical parameters were evaluated as potential prognostic indicators in wild Temminck's pangolins (Smutsia temminckii) rescued from the illegal wildlife trade. In this prospective cohort study, EDTA and lithium heparin blood samples were collected from the ventral coccygeal vein in 41 pangolins within 48 h of confiscation and before veterinary intervention. Receiving operator characteristic curve analysis was performed to assess each parameter as a prognostic indicator for release outcome and, where known, for 28 d survival postadmission. RBC counts, hemoglobin concentration, and Hct were significantly lower in female than in male pangolins. On intake, pangolins that later failed to be released had significantly lower mean corpuscular hemoglobin concentration (MCHC), higher blood urea nitrogen (BUN), and higher creatinine (Crea) values than individuals that were released. Individuals with lower MCHC or higher Crea values were also less likely to achieve 28-d survival. No pangolin with MCHC < 30.2 g/dl, BUN > 84 mg/dl, or Crea > 0.8 mg/dl was successfully rehabilitated. No other hematological or biochemical parameter, including plasma cortisol, total protein, lactate, lactate dehydrogenase, and glucose, showed significant prognostic value. This study suggests that MCHC, BUN, and Crea measured on intake are useful prognostic indicators for rehabilitation success and MCHC and Crea for short-term survival in Temminck's pangolins.

TOXICOLOGICAL IMPACT OF DICLOFENAC SODIUM: HEMATOLOGICAL, BIOCHEMICAL AND HISTOPATHOLOGICAL CHANGES IN MALE RATS

Background: Long-term administration of diclofenac sodium can lead to harmful effects on body tissues, causing cellular injuries that include changes in kidney function and contributing to the development of immune mechanisms against kidney tissues. This study aimed to evaluate the toxic effects of diclofenac sodium on hematological and biochemical parameters, as well as histopathological changes in the kidneys of experimental animals. Methods: Forty male Albino-Aster mice were divided into four groups: control and three groups administered diclofenac sodium orally at doses of 20.5, 45 and 56.25 mg/kg body weight for 20 days. Blood samples were collected for hematological and biochemical analyses and kidney tissues were examined histopathologically. Results: Diclofenac sodium administration caused a significant decrease in red blood cells, hemoglobin, packed cell volume, mean corpuscular volume and mean corpuscular hemoglobin concentration, as well as a significant increase in platelets and total white blood cells. Serum alkaline phosphatase, aspartate aminotransferase and alanine aminotransferase were significantly elevated. Histopathological examination revealed congestion, inflammation, glomerular abnormalities, tubular necrosis and vascular changes in the kidney tissues, with the severity increasing with higher doses. Conclusion: Diclofenac sodium administration at different doses can cause significant alterations in hematological and biochemical parameters, as well as histopathological changes in the kidneys of male rats, indicating potential adverse effects on animal tissues. Caution is recommended in the clinical use of diclofenac sodium and the lowest effective dose and duration should be used to achieve the desired therapeutic effect.

Mining Hypertension Predictors using Decision Tree: Baseline Data of Kharameh Cohort Study

Introduction: Hypertension is a serious chronic disease and an important risk factor for many health problems. This study aimed to investigate the factors associated with hypertension using a decision-tree algorithm. Methods: Methods: This cross-sectional study was conducted through the census in Kharameh City between 2014 and 2017. The study included 2510 hypertensive and 7840 non-hypertensive individuals. To create the decision tree, 70% of the cases were randomly allocated to the training dataset. In comparison, the remaining 30% were used as the testing dataset for the performance evaluation of the decision tree. Two models were assessed. In the first model (model I), 15 variables including age, gender, body mass index (BMI), years of education, occupation status, marital status, family history of hypertension, physical activity, total energy, number of meals, salt, oil type, drug use, alcohol use, and smoke entered into the model. in the second model (model II) 16 variables including age, gender, BMI and Blood factors as Hematocrit (HCT), Mean corpuscular hemoglobin concentration (MCHC), Platelet Count (PLT), Fasting blood sugar (FBS), Blood Urea Nitrogen (BUN), creatinine (Cr), triglycerides (TG), cholesterol (CHOL), Alkaline phosphatase (ALP), High-density lipoprotein (HDL), Gamma-glutamyl transpeptidase (GGT), low-density lipoproteins (LDL) and Urinary specific gravity (SG) were considered. A confusion matrix was used to measure the performance of the decision tree. Additionally, accuracy, sensitivity, specificity, and the receiver operating characteristics (ROC) curve were determined to compare the models. Results: For the model I, the accuracy, sensitivity, specificity and AUC value were 79.2%(77.8-80.6), 82.4%(80.1-84.5), 78.24%(76.4-80), and 0.80%(0.79-0.82), respectively. For model II, the corresponding values were 79.5%(78.2-80.8), 81.0%(78.3-83.6)79.0%(77.5-80.5)and 0.80%(0.79-0.81), respectively. Confusion matrix of model I showed that of the 1188 cases with hypertension in the training data set, 979 cases were classified correctly and, for model II of the 2812 non-hypertension cases, 2222 cases were classified correctly. Conclusion: We have suggested a decision tree model to identify the risk factors associated with hypertension. This model can be useful for early screening and improving preventive and curative health services in health promotion

Influence of the prebiotic Immunobeta and the combination Immunobeta + Zoovit probiotic on haematological parameters in breed Ile-de-France lambs

Abstract. The aim of the study was to evaluate the effect from supplementation of the prebiotic Immunobeta and the probiotic Zoovit on haematological parameters in Ile-de-France lambs. The experiment was conducted in the experimental farm of the Agricultural Institute, Stara Zagora. A total of 45 lambs divided into 3 groups of 15 animals each with similar initial live body weight, sex and birth type were included – one control and two experimental. Each animal from experimental group I was supplemented once daily with 8 g prebiotic Immunobeta and those from experimental group II received the same amount of the prebiotic plus 4 g probiotic Zoovit. Blood samples for analysis were collected from 8 animals per group in the beginning and the end of the trial. The group supplemented with the combination Immunobeta + Zoovit had statistically significantly higher haemoglobin content (P&lt;0.05) and haematocrit (P=0.013) that the group fed a diet with Immunobeta prebiotic. The mean corpuscular volume was lower (P&lt;0.05) in experimental group I vs. controls. The addition of the Immunobeta prebiotic resulted in reduced haemoglobin (Р=0.012), haematocrit (Р=0.012), mean corpuscular volume (Р&lt;0.001), mean corpuscular haemoglobin concentration (Р&lt;0.001), mean platelet volume (Р=0.002), platelet distribution width (Р=0.001) and volume of platelet participation (Р=0.002) at the end of the trial period. After the supplementation with synbiotic, there was a trend towards lower haemoglobin content (Р=0.088). The Immunobeta + Zoovit addition to the diet resulted in statistically significantly reduced haematocrit (Р&lt;0.05), reduced mean corpuscular volume, mean corpuscular haemoglobin concentration, mean platelet volume (Р&lt;0.001), platelet distribution width (Р&lt;0.01) and volume of platelet participation levels (Р&lt;0.05).

Enhancing diagnostic accuracy for iron deficiency in pregnant women through mean reticulocyte volume.

Women are more prone to iron deficiency (ID) anemia when pregnant. The diagnostic use of mean reticulocyte volume (MRV) in identifying ID anemia during pregnancy has not been thoroughly investigated. The objective of this study is to evaluate the effectiveness of MRV in diagnosing ID in pregnant women. Firstly, MRV of 20 healthy female volunteers (healthy group) was measured on specific days for one month. Subsequently, clinical data from 724 pregnant women were thoroughly examined. These women were divided into two groups: 282 with ID (research group) and 442 without ID (control group). Parameters such as MRV, reticulocyte hemoglobin equivalent (RHE), red blood cell volume distribution width-standard deviation (RDW-SD), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), hematocrit (HCT), reticulocyte count (RET), MRV/MCV ratio, and serum ferritin (SF) were analyzed and compared. MRV remained consistent over a period of one month for 20 healthy individuals. In addition, there were significant differences in MRV, RHE, RDW-SD, MCV, MCH, MCHC, HCT, RET, and MRV/MCV between the research group and control group. The receiver operating characteristic (ROC) analysis showed that the areas under the curve (AUCs) for these measures were as follow: 0.840, 0.837, 0.676, 0.654, 0.639, 0.602, 0.571, 0.550, and 0.816, respectively. Ultimately, there was a substantial disparity in MRV prior to and following therapy with oral iron treatments. In healthy women, MRV remains stable and is a reliable ID marker, which can be used to assess oral iron treatment effectiveness during pregnancy.

Hepatotoxic Effects of Gaseous Sulfur Dioxide (SO₂), Nitrogen Dioxide (NO₂), and Their Mixture on Sea Bass (Centropristis striata): Hematological, Biochemical and Genotoxic Studies

This study meticulously explores the intricate hepatotoxic effects stemming from acute exposure to gaseous sulfur dioxide (SO2), nitrogen dioxide (NO2), and their amalgamation on sea bass (Centropristis striata). Employing a comprehensive approach involving hematological, cytotoxic, and histochemical analyses, the research provides crucial insights into the potential adverse impacts of these pollutants on fish health. The examination specifically focuses on the effects of SO2+NO2 on hematological, histochemical, and serum biochemical parameters in Centropristis striata. Treatment groups, subjected to LC30, LC50, and LC90 acute exposure of gaseous SO2, NO2, and SO2+NO2, alongside a control group, underwent evaluation of parameters such as red and white blood cells, hemoglobin, hematocrit, mean corpuscular hemoglobin, mean corpuscular volume, mean corpuscular hemoglobin concentration, alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, total protein, albumin, serum creatinine, and blood urea. At the 96th hour, RBC values decreased, and WBC values increased in all experimental conditions compared to the control group (p&gt;0.05). MCV and MCH increased with the concentration of gaseous pollutants and exposure time (p&gt;0.05). Hematological parameter variations underscore disruptions in blood composition and immune responses. Simultaneously, alterations in serum biochemical parameters suggest potential impairments in liver and kidney functions, along with disturbances in lipid metabolism. Significant declines in albumin levels, indicating potential liver dysfunction or inflammation due to SO2 and NO2 exposures, were observed at all experimental conditions, while decreased globulin levels suggest immunosuppressive effects from combined pollutants. A substantial increase in the albumin/globulin ratio further signals an imbalance indicative of potential liver dysfunction or inflammation. Varied responses in liver enzyme levels (SGPT/ALT, SGOT/AST, ALP) underscore potential liver damage or injury (p&lt; 0.05). These findings deepen our understanding of environmental impacts on aquatic ecosystems, emphasizing the need for ongoing efforts to ensure the health and sustainability of fish populations in polluted environments.

The effect of an antioxidant supplement based on selenium, electrolytes, and vitamins on relieving transportation stress in goats

This study aimed to evaluate the efficacy of an antioxidant supplement (Transcare) based on selenium (Se), electolytes, and vitamins to relieve transportation stress in Nandidurga goats. Thirty 8-10-month-old Nandidurga female goats were randomly divided into two groups: non-supplemented (NS) (n=15) and supplemented (S) (n=15). S goats received orally antioxidant powder at a dose of 0.7g/kg BW, dissolved in 10mL drinking water, 45-60min before the seven-hour trip. The antioxidant supplementation influenced (P < 0.05) respiration rate and pulse rate after transport, head and shoulder temperature, mean corpuscular hemoglobin before transport, lymphocytes after transport, monocytes after transport, cortisol, aldosterone, tri-iodothyronine, thyroxine, glucose, HSP70, HSP90, and HSP27 after transportation. Antioxidant supplementation decreased transportation stress in Nandidurga goats.Thus, oral antioxidant supplementation proved beneficial and promising in alleviating transportation stress. Therefore, promoting such supplements among resource-poor farmers may help reverse the financial loss experienced due to transportation stress in goats.

Co-Administration of Hibiscus sabdariffa linn and Daflon-500® mitigates weight loss, hyperglycemia, hematological and oxidative changes in male rats with type-2 diabetes

BackgroundDiabetes mellitus is a complex metabolic disorder associated with persistent hyperglycemia and metabolic imbalance. Flavonoid compounds possess notable qualities in mitigating diabetes and its complications. AimHibiscus sabdariffa linn and daflon-500® were assessed for their effects on body weight, blood glucose/indices, and oxidative changes in type-2 diabetes rats over 28 days. Methods35 male rats were randomly divided into seven groups (n=5). Diabetes mellitus was induced in groups 2-7 via a single intraperitoneal injection of streptozotocin (STZ; 55mg/kg). Group 1(DW) received distilled water (2mL/kg) only. Group 2 (STZ; untreated), group 3 {MET; metformin (200mg/kg)}, group 4 {HS; Hibiscus sabdariffa (400mg/kg)}, group 5 {DAF; daflon-500® (1000mg/kg)}, group 6 {DAF+HS; daflon-500® (1000mg/kg) and Hibiscus sabdariffa (400mg/kg)}, group 7 {DAF+MET+HS; daflon-500® (1000mg/kg), metformin (200mg/kg), and Hibiscus sabdariffa (400mg/kg)}. Body weight and blood glucose were assessed fortnightly. Blood and hepatic/pancreatic tissues were collected for hematological and antioxidant testing. ResultsGroups 2-7 revealed a significant (P<0.05) decrease in body weight on day 14 compared to the increased growth rate observed on day 28 (Group 7).Blood glucose levels significantly increased on days 1 and 14 in groups 2-7 but declined on day 28 in groups 4-7. Significantly higher packed cell volume, hemoglobin levels, red blood cell counts, lymphocytes, and mean corpuscular hemoglobin were observed in groups 5-7. Platelet counts were higher in Groups 2 and 5. Untreated group 2 exhibited considerably reduced catalase activity, glutathione, and malondialdehyde concentrations compared to the treated groups. ConclusionHibiscus sabdariffa linn and Daflon-500® expressed substantial hypoglycemic, antioxidant, and hemato-protective effects.

The effect of different dietary manipulations on haematological properties in Japanese quail

Quantitative and qualitative dietary conditions are crucial for physiological functions. Blood haematology, the indicator of immune regulation, is critically affected by dietary conditions. Despite their importance, the effect of macro- and micronutrient manipulation remains unknown. We used feed restriction, energy or protein restriction, and supplementation of leucine, methionine, or both on top of restricted-feeding to study the effect on haematological properties in Japanese quail. Fifty-six birds of six weeks of age were distributed into seven treatments; control, 20% feed restriction, 20% energy restriction, 20% protein restriction, supplementing 20% leucine, 20% methionine, or both on top of restricted feeding. Haematological properties, including white blood cell count, number and percent of lymphocytes, mid-range (eosinophil + basophil), granulocytes, red blood cell count, haemoglobin levels, packed cell volume, mean corpuscular volume, and mean corpuscular haemoglobin were analysed. We found that quantitative feed restriction, energy restriction, and amino acid supplementations showed no significant effect compared to the control group. However, protein restriction reduced all indices, suggesting the importance of proteins in maintaining leukocyte and erythrocyte indices. The present study revealed that neither moderate calorie restriction nor moderate specific amino acid supplementation have an impact on blood haematology, while 20% protein restriction has a notable effect.

Hemoglobin levels in red blood cells and risk of colorectal cancer: A causal investigation based on Mendelian randomization.

Mean corpuscular hemoglobin (MCH) is a critical parameter in red blood cells, associated with various diseases. While studies suggest a potential link between MCH levels and colorectal cancer (CRC), observational studies are insufficient to establish causality directly. This study utilized a 2-sample Mendelian randomization (MR) approach to investigate the genetic causal relationship between MCH and colorectal cancer (CRC). Genome-wide association study (GWAS) summary data for both MCH and CRC were sourced from relevant databases. MR analyses were performed using methods including inverse variance weighted (IVW), MR Egger, weighted median, simple mode, and weighted mode. Cochrane's Q test was applied to assess heterogeneity in the MR findings. Horizontal pleiotropy was evaluated using the MR-Egger intercept test and the MR Pleiotropy RESidual Sum and Outlier (MR-PRESSO) test. Additionally, a leave-one-out analysis was conducted to assess the robustness of this association. The IVW method demonstrated that MCH is an independent risk factor for colorectal cancer (P = .013). Horizontal pleiotropy is unlikely to influence the causal relationship (P > .05), and there was no evidence of heterogeneity among the genetic variants (P > .05). Lastly, the leave-one-out test confirmed the stability and robustness of the association. All participants in the GWAS were derived from a specific population. Due to limitations inherent to the database, the Mendelian Randomization (MR) analysis was unable to incorporate stratified analyses by country, ethnicity, or age group.

Investigations into disease associations with feline immunodeficiency virus (FIV) infection in lions (Panthera leo) in Australia: a case-control study.

Feline immunodeficiency virus (FIV) is a highly mutable retrovirus with numerous species-specific strains, such as those infecting domestic cats (Felis catus, FIVFca) and wild populations of nondomestic cats including lions (Panthera leo, FIVPle). When FIVFca infection manifests as disease in domestic cats, common presentations include lymphadenopathy, lymphoid malignancies, oral cavity disease, immunological dysfunctions and neurological abnormalities. In contrast to domestic cats, there has been little published evidence of direct FIVPle disease associations in lions. The objective of the study, therefore, was to conduct a longitudinal case-control comparison of clinical findings and survival analysis of FIVPle-infected and FIVPle-uninfected captive lions to investigate possible FIVPle disease associations in nondomestic felids in Australia. Between 2005 and 2023, 38 lions were recruited from zoos across Australia. Lions had been FIV tested as part of a routine diagnostic service. The 38 lions included in the study comprised 15 FIV-infected (9 males, 6 females) and 23 FIV-uninfected (9 males, 14 females) animals. Lifetime longitudinal clinical histories and clinicopathological data collected by zoos for these lions were interrogated, including survival data. FIV-infected lions were more likely to be reported with lower mean corpuscular volume (MCV) (fL) (P = 0.015), monocytes (%) (P = 0.023), chloride (mmol/L) (P = 0.016) and phosphate (mmol/L) (P = 0.029), but with a higher mean corpuscular hemoglobin concentration (MCHC) (g/L) (P = 0.001) and bicarbonate (mmol/L) (P = 0.035), than FIV-uninfected lions. The disease associations usually seen in domestic cats with FIV infection were not observed in lions, and there was no difference in survival between FIV-infected and FIV-uninfected lions. The main limitation of the study was a small sample size dictated by the number of lions able to be recruited from zoos in Australia over an 18-year period. Few differences were found between case and control groups, suggesting that FIV-infected lions did not experience clinicopathological changes that predisposed them to future disease compared with FIV-uninfected lions. Further research will help to determine whether these results are indicative of a general absence of FIV-associated disease in captive lions, or the result of nonpathogenic FIVPle subtype(s) present in Australia.

Exploring hematological diversity, a comprehensive study of CBC variations among the population of Tehsil Kot-Momin Sargodha

Objectives: To ascertain the diversity and patterns observed in the complete blood count within the population of Tehsil Kot-Momin , Sargodha. Study Design: Observational Cross Sectional Study Place of Study: Rai Medical College,Sargodha Materials &amp; Methods: A comprehensive collection of 401 CBC samples was meticoulously acquired from individuals at Medina Medical Complex in Kot-Momin ,Sargodha, subsequent to securing verbal informed consent. Subsequent research activities were conducted at Rai Medical College,Sargodha, post-recipt of sanction from the institutional ethical review committee. The time frame for data accumulation extended from june 2023 through August 2023 and the results were analyzed on SPSS Ver 27. Results: The study aimed to explore gender differences in hematological parameters among a sample of 401 individuals. Key findings indicated significant gender-based variations in hemoglobin (p &lt; 0.001), total RBC count ( p&lt; 0.001), hematocrit (p &lt;0.001) , platlelt count (p=0.008), and mean platelet volume (MPV) (p=0.033). In this investigation, male subjects were observed to possess higher mean concentration of hemoplobin , total erythrocyte count and hematocrit levels relative to their female counterparts, who demonstrated an augmented mean thrombocyte count. Significantly, the mean thrombocyte volume (MTV)-a proxy for platelet volume - was markedly distinct between genders, alluding to potential differences in thrombocyte function or production. Conversly, the study revealed no statistically significant disparities in parameters such as leukocyte count (WBC) , mean corpuscular volume ( MCV), mean corpuscular hemoglobin (MCH) , mean corpuscular hemoglobin concentration (MCHC), and proportions of hematocrit (PCT), as well as neutrophil,lymphocyte ,monocyte, eosinophil and basophil counts, thereby indicating a homogeneity in these hematological indices across genders. Conclusion: This study offers a comprehensive analysis of variations in hematological parameters across different groups, highlighting significant differences in hemoglobin , total RBC count, hematocrit, platelet count and mean platelet volume. These findings underscore the potential influence of biological and physiological factors on blood profiles, suggesting a need for personalized approaches in the interpretation of hematological data for clinical or diagnostic purposes.

Molecular and hematological spectrum of α-thalassemia in Saudi patients

α-thalassemia (α-thal) is a genetic disorder characterized by a decreased synthesis of α-globin chains. A deletion mutation most often causes it in one or more α-globin chains. No comprehensive characterization studies have been conducted on α-thal patients in the Saudi population. Therefore, this research aims to identify the spectrum of genetic mutations responsible for α-thal in our region. Individuals with microcytic, hypochromic red blood cells and normal hemoglobin (Hb) A2 were enrolled. Sixty samples of individuals suspected of α-thal were selected for further genetic analysis. Multiplex ligation-dependent probe amplification assay was used to detect deletion mutations in α-globin genes. Among all samples tested, the -α3.7 deletion mutation was detected in 57 (95%) cases, whereas no mutation was detected in the remaining 3 (5%). In addition, 9 (15%) individuals were heterozygous for -α3.7, while -α3.7 homozygosity was found in 85% of the analyzed cases. The hematological characteristics of α3.7 subjects were significantly lower than the control group in the mean of Hb, hematocrit, mean corpuscular volume, mean corpuscular Hb, and mean corpuscular Hb concentration (p&lt;0.001). These results highlight the importance of α-thal diagnosis and investigation in Saudi Arabia’s pre-marital screening program for microcytic hypochromic individuals. Thus, it contributes to reducing the spread of genetic diseases.

Association Between Anthropometric and Hematological Parameters and the Diagnosis of Intestinal Parasitosis in Low-Income Children

Objective: This study aims to determine the association between anthropometric values and laboratory tests with parasitosis diagnosis and identify diagnostic models for parasitosis without relying on copro-parasitological examinations. Methods: Data were collected from 1894 children aged 0–14 who attended a medical center for low-income children in Lima, Peru, between 2021 and 2022. Anthropometric data (BMI, weight, height), laboratory data (red blood cells, hemoglobin, platelets, hematocrit, mean corpuscular volume, mean corpuscular hemoglobin, eosinophils), and parasitological examination results were analyzed. Prevalence ratios for the association between parasitosis and each anthropometric and laboratory variable were estimated using multivariable Poisson regression. Regression models were developed for each type of parasite found, and the diagnostic value was assessed using ROC curves. Results: A high prevalence of parasitosis was identified (41.9%), including Blastocystis hominis (29.1%), Endolimax nana (7.76%), Entamoeba coli (5.97%), Giardia duodenalis (6.44%), and Enterobius vermicularis (1.85%). It was found that the male sex (PRa 1.18), the age group of 2–5 years (PRa 4.83) and &gt;5 years (PRa 4.59), the percentage of eosinophils (PRa 1.02 for every 1% increase), and height/age with −5 SD (PRa 1.34) were associated with a greater risk of parasitosis. Satisfactory values were only shown for diagnostic models associating Enterobius vermicularis and BMI, with a diagnostic value of 70.9% and 70.2% for a BMI &lt; 12 and hematocrit &gt; 29.8%, and BMI &lt; 12 and hemoglobin &lt; 10.6 g/L, respectively. Conclusions: Satisfactory diagnostic value models were only found for parasitosis by Enterobius vermicularis, suggesting the potential for reducing reliance on copro-parasitological exams in resource-limited settings.

Haemoglobin types and variant interference with HbA1c and its association with uncontrolled HbA1c in type 2 diabetes mellitus

Diabetes mellitus is among the leading global health concerns, causing over 1.5 million deaths alongside other significant comorbidities and complications. Conventional diagnosis involves estimating fasting, random blood glucose levels and glucose tolerance test. For monitoring purposes, long-term glycaemic control has been achieved through the measurement of glycated haemoglobin (HbA1c) which is considered reliable and preferred tool. However, its estimation could be affected by haemoglobin types like HbA0, HbA2, and HbF concentrations whose magnitude remains unclear as well as other haematological parameters. As such, the current study determined the association between HbA1c and haemoglobin types and determined correlation between haemoglobin types and haematological parameters among patients with type 2 diabetes mellitus (T2DM) compared to healthy non-diabetic participants. In this cross-sectional study, participants [n = 144 (72 per group), ages 23–80 years] were recruited and the desired parameter measured. HbA1c and other Haemoglobin variants were measured using ion-exchange high-performance liquid chromatography (HPLC) by the Bio-Rad D-10 machine (Bio-Rad Laboratories, Inc). Haematological parameters were measured using the Celtac G MEK-i machine (Nihon Kohden Europe). SPSS version 27 (IBM Corporation, Chicago, Illinois, United States) was used for the analysis. Chi-square (χ2) analysis, Mann-Whitney U test, Binary logistic regression and Pearson correlation were used to determine the differences between proportions, compare laboratory characteristics, associations and correlations respectively. With non-diabetics as the reference group, HbA1c was associated with increased HbA0 [OR = 1.509, 95% CI = 1.020–1.099, p = 0.003] and increased HbA2 [OR = 3.893, 95% CI = 2.161–7.014, p = 0.001]. However, there was no significant association between HbA1c and HbF [OR = 2.062, 95% CI = 0.873–4.875, p = 0.099]. Further, haematocrit (HCT) had a negative correlation with HbAO and a positive correlation with HbAS in participants with controlled diabetes. Mean cell volume (MCV) and mean cell haemoglobin (MCH) had a negative correlation with HbF. MCHC (mean cell haemoglobin concentration) had a negative correlation with HbA2 in participant with uncontrolled diabetes. The study concluded that levels of various haemoglobin types should be considered while monitoring glycaemic control through HbA1c. Additionally, MCHC should be considered in individuals with high concentration of HbA2 among T2DM patients while interpretating results for HbA1c.

Significant haematological alterations in clozapine-treated patients: prevalence and clinical correlation.

Clozapine is an atypical antipsychotic crucial for treatment-resistant schizophrenia, characterised by its multi-receptor targeting, including serotonin (5-HT2A, 5-HT2C) and dopamine (D1, D2, D3, D4) receptors, among others. This broad mechanism is effective against positive symptoms of schizophrenia with a lower incidence of extrapyramidal side effects. However, clozapine poses significant haematological risks, notably agranulocytosis, necessitating stringent blood monitoring protocols. This study examined haematological parameters in 157 patients on clozapine therapy, analysing the prevalence and clinical correlations of haematological abnormalities such as leucocytosis, thrombocytosis, and alterations in red blood cell distribution width (RDW) and mean platelet volume (MPV). The findings revealed leucocytosis in 36.9% of patients, thrombocytosis in 8.9%, and elevated RDW in 23.6%. Notably, higher clozapine doses were associated with leucocytosis, though no significant correlations were found between clozapine dose, duration of use, and changes in RDW, mean corpuscular haemoglobin concentration, or MPV. The study's results underscore the necessity of regular haematological monitoring to mitigate the risks of clozapine therapy while leveraging its therapeutic benefits. Additionally, the study suggests personalised dosing strategies to balance efficacy and safety, particularly in managing clozapine-induced haematological changes.

Hematological parameters of the European hake (Merluccius merluccius) in Toroneos Gulf, northern Greece: A case study

Background: The European hake (Merluccius merluccius) is a commercially valuable demersal species widely distributed in the Mediterranean Sea. Assessing the condition of fish populations in their natural habitats is challenging due to the lack of reliable reference points. Objective: This study aimed to utilize hematological analysis as an economical method to evaluate the physiological and health status of European hake, addressing the gap in hematological data for this species. Methods: Blood samples were collected from the caudal vein of 40 adult European hakes caught from the Toroneos Gulf (northern Greece) using a commercial bottom otter trawl. An automated hematological analyzer was used to assess hematological parameters alongside biometric and biological indices. Results: Female hakes showed significantly higher white blood cell (WBC) counts, thrombocyte (TC) counts, and red cell distribution width (RDW) than their male counterparts. Strong correlations were observed among various hematological parameters, notably between WBC and red blood cells (RBCs), hematocrit (Ht), and hemoglobin (Hb); between RBC and both Ht and Hb; between TC and both mean platelet volume and platelet distribution width (PDW); and between mean corpuscular Hb concentration and RDW. Significant differences were noted in RBCs, Hb, and Ht compared to data from wild-caught European hake populations in Argentina and Denmark. Both trawling depth and duration were found to significantly affect RBC, WBC, Hb, and Ht values, while having no notable impact on TC. Fish captured at an average depth of 80 m and with a trawling duration of 30 min exhibited significantly elevated hematological indices. Conclusion: This study demonstrates that hematological analysis is a valuable, cost-effective tool for assessing the physiological and health status of European hake populations in the Mediterranean. Notable differences in hematological parameters based on sex, as well as significant correlations among key blood metrics, underscore the importance of understanding species-specific hematological profiles. The influence of trawling depth and duration on certain blood parameters highlights the need for standardized sampling protocols in population health assessments. These findings contribute essential baseline hematological data for European hake, facilitating more informed fisheries management and conservation strategies.

Use of agrochemicals resulted in anemia and inflammation among farmers in Fako Division, Cameroon

The use of agrochemicals as plant growth regulators, pesticides, and soil fertilizers can result in insults among farmers and other non-targeted organisms. The adverse effects of agrochemicals are of global concern, though limited studies have delineated their toxicity on blood cells and inflammatory parameters in Fako division in Cameroon. This study examined the impact of occupational exposure on haematological and inflammatory parameters among farmers in Fako division. Briefly, 165 farmers who occupationally applied agrochemicals and a reference population of 75 non-farmers were interviewed on types of agrochemicals used, knowledge and safety during use of agrochemicals, as well as related symptoms of exposure. Serum cholinesterase (acetylcholinesterase and butyrylcholinesterase) activities were measured as biomarkers of exposure to agrochemicals. Complete blood count and serum levels of interleukin-6 (IL-6) and nitric oxide (NO) were determined as haematological and inflammatory biomarkers. Results disclosed frequent use of insecticides, fungicides, herbicides and fertilizers with neglect of personal protective equipment. Reported symptoms of exposure to agrochemicals were consistent with decrease in cholinesterase activities. Exposure to agrochemicals decreased erythrocyte count and red cell distribution width, as well as increased mean cell volume, mean corpuscular hemoglobin, serum levels of NO and IL-6 in farmers; suggesting anemia and increased cellular inflammation. In conclusion, the use of agrochemicals resulted in inhibited cholinesterase activities, induced anemia, and promoted cell inflammation in farmers. These findings call for more sensitization and training of the farmers to minimize agrochemical exposure and related health hazards.

Effect of Iron Deficiency Anemia on Insulin Like- Growth Factor-1 Level Among Children: A Cross-Sectional Study

Objective: To assess the effect of iron deficiency anemia on the serum level of IGF-1 by comparison between low ferritin and low hemoglobin group and healthy control. Study Design: Comparative case control study. Place and Duration: Study The study was conducted at department of Chemical Pathology and Endocrinology, Armed Forces Institute of Pathology, Rawalpindi, from Jul to Dec 2022. Methodology: The case control study was done in 110 children, aged between 2-14 years. Children with short stature and iron deficiency anemia (cases) were compared to a group of age and gender matched healthy children (controls). The sample size of 110 children was calculated using WHO sample size calculator. After brief history and examination, 3ml venous blood was drawn by standard technique. Complete blood picture estimation and the estimation of IGF-1 and ferritin levels was done. Parameters of complete blood picture included were Hemoglobin in (Hb), Red blood cells (RBC), Packed cell volume (PCV), Mean corpuscular hemoglobin (MCH), Mean corpuscular volume (MCV). Data was analyzed using SPSS. Results: Comparison of the serum levels of IGF-1, analyzed by chemiluminescent immunoassay between two groups showed lower IGF-1 levels among iron deficiency anemia group (8.60 µmol/L) as compared to control group (38.80 µmol/L) which is statistically significant (p-value&lt;0.001). Results showed a positive correlation of Hemoglobin and serum ferritin with Serum IGF-1 levels with a r value of 0.78 and 0.81 (p-value 0.001) respectively. Conclusion: There is a strong correlation between Iron Deficiency anemia and IGF-1 levels. Children with Iron deficiency anemia have